FastQCFastQC Report
Thu 26 May 2016
SRR937210_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937210_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401829
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38870.9673268977599925No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33520.8341856859509891No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23530.5855724698814669No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8090.20132942122146483No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6870.17096824768744914No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12800.057.9032141
GTATCAA24400.045.3684731
TAGAGCG459.619078E-442.2208985
GTACGCT459.619078E-442.2208983
GTGGTAT5300.038.546121
GTGTACG756.9007474E-638.0082661
TGGTATC5400.036.9432832
TATCAAC31750.034.557972
TCAACGC32100.034.1811684
ATCAACG32400.033.8646773
CAACGCA32800.033.451695
AACGCAG33900.032.366246
CCGCGCC600.00394612331.6696119
GGACCGA902.4096045E-531.6656726
GTATAGA650.005833404629.2371271
ACTATAC1153.8572907E-628.9121343
ACGCAGA38500.028.3792637
CGCAGAG38350.027.9947818
TAGACTA908.939021E-426.388065
GCAGAGT41200.025.7123439
Produced by FastQC (version 0.11.5)