FastQCFastQC Report
Thu 26 May 2016
SRR937209_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937209_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509421
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28930.5678996350758999No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22560.44285571266202217No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15530.30485590503728743No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7050.13839241020688192No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6000.11778077464415485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13950.050.7829171
GCCGTTC459.6216676E-442.2207378
GTATCAA24900.040.862041
TGCACCG756.88838E-638.0210955
ATCAACG31550.032.0922623
TCAACGC31800.031.8399684
CAACGCA32450.031.0495855
TATCAAC32650.030.8654612
AACGCAG33100.030.4218886
ACGCAGA37500.026.7257257
CTAATAC1806.6265784E-926.4087373
CGCAGAG37950.026.283668
ATAGCAC1109.41817E-525.9285763
GGGCGCG950.001225865124.999127
GTATAGA1151.2721577E-424.806131
GTACATG26850.024.7907331
GCAGAGT39950.024.1308259
GAGTACT22750.023.79901912-13
ACCCGGG1401.7774348E-523.7491637
TACATGG27100.023.6801592
Produced by FastQC (version 0.11.5)