##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937209_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 509421 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.6545313208525 33.0 31.0 34.0 30.0 34.0 2 31.73023687676794 33.0 31.0 34.0 30.0 34.0 3 31.77363320318558 33.0 31.0 34.0 30.0 34.0 4 35.41395231056435 37.0 35.0 37.0 33.0 37.0 5 35.38324293658879 37.0 35.0 37.0 33.0 37.0 6 35.4727465102538 37.0 35.0 37.0 33.0 37.0 7 35.40683835177584 37.0 35.0 37.0 33.0 37.0 8 35.40072160354599 37.0 35.0 37.0 33.0 37.0 9 37.00088531882274 39.0 37.0 39.0 33.0 39.0 10-11 36.91932507690103 39.0 37.0 39.0 33.0 39.0 12-13 36.79537946021071 39.0 37.0 39.0 33.0 39.0 14-15 37.937913827659244 40.0 38.0 41.0 33.0 41.0 16-17 37.87313440160496 40.0 38.0 41.0 33.0 41.0 18-19 37.844266333739675 40.0 38.0 41.0 33.0 41.0 20-21 37.778666368288704 40.0 38.0 41.0 32.5 41.0 22-23 37.65081337439956 40.0 37.0 41.0 32.0 41.0 24-25 37.508721666362405 40.0 37.0 41.0 32.0 41.0 26-27 37.348731206605144 40.0 37.0 41.0 32.0 41.0 28-29 37.153610667797366 39.0 36.5 41.0 31.0 41.0 30-31 37.111034880776415 39.0 36.5 41.0 31.0 41.0 32-33 36.93409184152205 39.0 36.0 41.0 31.0 41.0 34-35 36.738687647348655 39.0 35.5 41.0 30.5 41.0 36-37 36.736583297508346 39.0 36.0 41.0 30.0 41.0 38-39 36.70724508805094 39.0 36.0 41.0 30.0 41.0 40-41 36.530164637892824 39.0 35.5 41.0 30.0 41.0 42-43 36.28085709069708 39.0 35.0 41.0 30.0 41.0 44-45 36.02889456853958 39.0 35.0 41.0 29.5 41.0 46-47 35.65634514478202 39.0 35.0 41.0 27.5 41.0 48-49 35.30829608516335 39.0 35.0 40.5 26.0 41.0 50-51 34.357951478246875 38.0 34.0 39.5 25.0 40.5 52-53 34.47400970906186 38.0 34.0 39.5 24.5 40.5 54-55 34.810144261818806 38.0 35.0 40.0 25.0 41.0 56-57 34.58490914194742 38.0 34.0 40.0 24.0 41.0 58-59 34.34062887081608 38.0 34.0 40.0 23.0 41.0 60-61 34.06448104809185 38.0 34.0 40.0 22.5 41.0 62-63 33.72530578833617 37.0 33.5 40.0 22.0 41.0 64-65 33.352589508481195 37.0 33.0 40.0 21.0 41.0 66-67 32.97185333937941 36.0 33.0 39.0 19.5 41.0 68-69 32.474646706751386 35.5 32.0 39.0 18.0 40.5 70-71 32.05142897524837 35.0 32.0 38.5 15.5 40.0 72-73 31.639406306375278 35.0 31.0 37.5 11.5 40.0 74-75 31.193854395480358 35.0 31.0 37.0 7.5 39.0 76-77 30.764381523337278 35.0 31.0 36.5 7.0 39.0 78-79 30.29706961432685 34.5 30.0 36.0 2.0 38.5 80-81 29.86394063063753 34.0 30.0 35.5 2.0 37.5 82-83 29.430535843634246 34.0 29.0 35.0 2.0 37.0 84-85 28.994400505672125 34.0 29.0 35.0 2.0 36.5 86-87 28.57963256324337 34.0 29.0 35.0 2.0 36.0 88-89 28.185815857610898 34.0 28.5 35.0 2.0 36.0 90-91 27.770586607148118 34.0 27.0 35.0 2.0 35.0 92-93 27.343107174615888 33.0 26.5 35.0 2.0 35.0 94-95 26.810359211732536 33.0 25.5 35.0 2.0 35.0 96-97 26.193946657087164 33.0 24.5 35.0 2.0 35.0 98-99 25.495165099200857 32.5 21.5 35.0 2.0 35.0 100-101 23.68771409109558 30.5 9.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.017387851414646605 1101 2 -0.18910710973327838 1101 3 0.07572535943680236 1101 4 -0.03118331158739096 1101 5 0.12570264294060962 1101 6 -0.23326576925235543 1101 7 -0.04486674989090034 1101 8 -0.004485032707364667 1101 9 -0.04523551643841017 1101 10-11 -0.05934292452327128 1101 12-13 -0.06939500768592666 1101 14-15 0.13981452818953954 1101 16-17 0.0808689017308808 1101 18-19 0.15087408770617827 1101 20-21 0.26671975305463036 1101 22-23 -0.041811907719583985 1101 24-25 -0.01352197662664878 1101 26-27 0.06426198571488584 1101 28-29 0.1110247010440304 1101 30-31 0.18407576977936912 1101 32-33 0.32120440485690693 1101 34-35 0.18986494587481673 1101 36-37 0.1827956382906848 1101 38-39 0.24522732556263094 1101 40-41 0.3370053076121735 1101 42-43 0.4254313431532779 1101 44-45 0.32687396014272707 1101 46-47 0.24937846633475402 1101 48-49 0.4858294086330659 1101 50-51 0.3519185287573592 1101 52-53 0.40038580121755984 1101 54-55 0.5502368872615904 1101 56-57 0.4448978356243245 1101 58-59 0.6214348559720619 1101 60-61 0.42698488504340304 1101 62-63 0.16174406127721142 1101 64-65 0.11903388612285681 1101 66-67 0.2918999423487989 1101 68-69 0.3211203869950907 1101 70-71 0.3018130917375217 1101 72-73 0.1645145756035724 1101 74-75 0.35944123697447594 1101 76-77 0.47400477156395127 1101 78-79 0.6116040976150714 1101 80-81 0.6197053503710315 1101 82-83 0.803001041198705 1101 84-85 0.7147065480216348 1101 86-87 0.7346618340640418 1101 88-89 0.8025275028394638 1101 90-91 0.9268627413085575 1101 92-93 0.532178723352164 1101 94-95 0.5248342156094168 1101 96-97 0.30236235909674747 1101 98-99 0.38735864697372335 1101 100-101 0.7909872838878478 1102 1 -0.1346995007688392 1102 2 -0.15611837317213784 1102 3 -0.12802471287067263 1102 4 -0.1618078002702319 1102 5 -0.06635427750464373 1102 6 -0.12257335286045645 1102 7 -0.014324071952536599 1102 8 0.015554467833908348 1102 9 0.08630624422797695 1102 10-11 -0.10546269875866443 1102 12-13 -0.08875688387900027 1102 14-15 -0.08309082752192154 1102 16-17 -0.07476570999862986 1102 18-19 0.11772007691376984 1102 20-21 -0.002020278843303913 1102 22-23 -0.06724761191176754 1102 24-25 -0.01911650935507936 1102 26-27 0.24655946280374508 1102 28-29 -0.1315606837557013 1102 30-31 -0.04351097853276542 1102 32-33 -0.2159822971784351 1102 34-35 -0.013252952498056914 1102 36-37 -0.11730517899039228 1102 38-39 0.022169091187244305 1102 40-41 -0.26564238557273967 1102 42-43 -0.03459708401450001 1102 44-45 -0.19771471361420367 1102 46-47 0.11178353694817389 1102 48-49 0.10747950655804317 1102 50-51 0.04951626780587759 1102 52-53 0.06730037923113485 1102 54-55 -0.10056345320332127 1102 56-57 -0.1822517623461053 1102 58-59 -0.1740258872661542 1102 60-61 0.032008395302803194 1102 62-63 -0.08546073828646428 1102 64-65 0.008833160568975984 1102 66-67 0.19207017480700017 1102 68-69 -0.028166041511411777 1102 70-71 -0.06507917425481935 1102 72-73 -6.811504048087613E-4 1102 74-75 -0.037634235503233526 1102 76-77 -0.4296568790297961 1102 78-79 -0.41780231998332695 1102 80-81 -0.44533005963301875 1102 82-83 -0.2261368057738835 1102 84-85 0.12412800557508064 1102 86-87 0.1427032517863971 1102 88-89 0.43861843672150513 1102 90-91 0.2146506283601468 1102 92-93 -0.25336143415417567 1102 94-95 -0.28768696015146134 1102 96-97 -0.1047328636515985 1102 98-99 -0.22794905232633766 1102 100-101 -0.08182593143968475 1103 1 0.05622784190463648 1103 2 -0.09666069997734894 1103 3 0.12250994479254373 1103 4 -0.005089195430571181 1103 5 -0.12116370244369534 1103 6 -0.05653455822511688 1103 7 -0.08476794692681722 1103 8 -0.24583545503821114 1103 9 -0.030423708447806064 1103 10-11 -0.03438596369323221 1103 12-13 -0.06861915107689498 1103 14-15 0.013478010821287967 1103 16-17 -0.004747378878747099 1103 18-19 0.039362333262637605 1103 20-21 0.08071136977282833 1103 22-23 0.21642023318403147 1103 24-25 0.17632970377225377 1103 26-27 0.07829505822221705 1103 28-29 -0.0065240526913115104 1103 30-31 -0.04437297762244441 1103 32-33 -0.03889526176944713 1103 34-35 0.1949159307376931 1103 36-37 0.2876068129289564 1103 38-39 0.45102221153042166 1103 40-41 0.4750502065349025 1103 42-43 0.5191617308224821 1103 44-45 0.553867436613686 1103 46-47 0.37057187486428944 1103 48-49 0.2555640915826487 1103 50-51 0.15623424913805906 1103 52-53 0.07744952222009971 1103 54-55 0.1605942131328817 1103 56-57 0.23431826066131833 1103 58-59 0.22950347681857153 1103 60-61 0.2694197793023534 1103 62-63 0.3629284301416149 1103 64-65 0.4160271898613601 1103 66-67 0.6156437455719868 1103 68-69 0.7439622389173124 1103 70-71 0.8915605568887557 1103 72-73 0.7143104634195971 1103 74-75 0.7836030527380373 1103 76-77 0.8268151286019325 1103 78-79 0.7274125718693014 1103 80-81 0.6522395082139489 1103 82-83 0.8561911217496032 1103 84-85 0.7139738745262783 1103 86-87 0.5596162039072574 1103 88-89 0.5832004684829748 1103 90-91 0.6407026413540429 1103 92-93 0.7761834677369208 1103 94-95 0.8159431003911131 1103 96-97 0.9350453547381292 1103 98-99 0.8956238949311626 1103 100-101 1.4083460319230134 1104 1 -0.37015125943150196 1104 2 -0.44004727463731896 1104 3 -0.25743730233335427 1104 4 -0.2594153307496825 1104 5 -0.30292133229436047 1104 6 -0.3362513445626547 1104 7 -0.2084907545520025 1104 8 -0.1595725165965547 1104 9 -0.16378457274237235 1104 10-11 -0.27631650574233646 1104 12-13 -0.03364372912050584 1104 14-15 -0.06268728151755454 1104 16-17 -0.2929032929288695 1104 18-19 -0.12970996215346275 1104 20-21 -0.023545494095451147 1104 22-23 0.13969877102356776 1104 24-25 -0.1258503487141951 1104 26-27 -0.2915278999351685 1104 28-29 -0.23281523257062275 1104 30-31 -0.5094275288899723 1104 32-33 -0.28558155534718566 1104 34-35 -0.24044514669979122 1104 36-37 -0.20465676494147544 1104 38-39 0.03276918423480879 1104 40-41 -0.234962182397652 1104 42-43 -0.38921345401239904 1104 44-45 -0.6591589265019309 1104 46-47 -0.59684057001828 1104 48-49 -0.4352188090261464 1104 50-51 -0.6022914678880653 1104 52-53 -0.4628444818029678 1104 54-55 -0.7091088521288142 1104 56-57 -0.30683830613900653 1104 58-59 -0.44941002711791356 1104 60-61 -0.5450426342748571 1104 62-63 -0.16427393574486615 1104 64-65 -0.0627784104690079 1104 66-67 -0.0779732898763541 1104 68-69 0.04265207599619458 1104 70-71 -0.27916026584293974 1104 72-73 -0.3893602574031334 1104 74-75 -0.186437044774852 1104 76-77 -0.16270756445893042 1104 78-79 -0.4695281610759352 1104 80-81 -0.5110879192841544 1104 82-83 -0.3589000634582753 1104 84-85 -0.5505464872838353 1104 86-87 -0.7215859221934231 1104 88-89 -0.7665370992898097 1104 90-91 -0.6796878098428856 1104 92-93 -0.543980519101499 1104 94-95 -0.8567859283432178 1104 96-97 -0.5973989949761247 1104 98-99 -0.347395674253697 1104 100-101 0.14740247483401347 1105 1 0.2210491501802494 1105 2 0.083620162993995 1105 3 0.042232536470294235 1105 4 0.14186294040623437 1105 5 0.32745702890552053 1105 6 -0.2213040467643168 1105 7 0.25896100130526634 1105 8 0.11991688116823696 1105 9 0.2179223782984323 1105 10-11 0.09775436415296923 1105 12-13 0.37039765579094563 1105 14-15 0.22593893010341048 1105 16-17 0.45247974702593297 1105 18-19 0.29919944655785713 1105 20-21 0.27828275145972725 1105 22-23 0.30786911301406406 1105 24-25 0.26080019243235597 1105 26-27 0.4215187640242988 1105 28-29 0.4052830742497733 1105 30-31 0.3463564715337597 1105 32-33 0.17048670150762746 1105 34-35 0.7875523461937988 1105 36-37 0.7633379030434781 1105 38-39 0.5494538008417393 1105 40-41 0.5431456584893652 1105 42-43 0.8528635600591841 1105 44-45 0.7303827320725489 1105 46-47 0.6273688969567672 1105 48-49 0.7406140976282742 1105 50-51 0.6892391029200411 1105 52-53 0.7400987198778495 1105 54-55 0.927828593800669 1105 56-57 1.0330158579528685 1105 58-59 1.1306055098795582 1105 60-61 1.140302269413418 1105 62-63 1.3383788300172483 1105 64-65 1.0057962465694246 1105 66-67 1.2763497031143487 1105 68-69 1.3251076278244298 1105 70-71 1.3807604601585766 1105 72-73 1.2749611465764552 1105 74-75 1.358245064725665 1105 76-77 1.1881196040998354 1105 78-79 1.5936615138830135 1105 80-81 1.5036766422370569 1105 82-83 1.1682323011668068 1105 84-85 1.1564928319131802 1105 86-87 1.191998357190041 1105 88-89 1.3288432923131488 1105 90-91 1.8913762979273763 1105 92-93 1.7674259322835866 1105 94-95 1.97419625707672 1105 96-97 1.6727770321429958 1105 98-99 2.1026696517584114 1105 100-101 2.575676279103156 1106 1 0.22649528842932298 1106 2 0.1636122503300257 1106 3 0.07092830690413265 1106 4 0.18287897591431346 1106 5 0.23680139051426607 1106 6 0.17617750065603133 1106 7 0.2404589675033577 1106 8 0.17384663154841462 1106 9 0.3315432875129787 1106 10-11 0.3811822785478327 1106 12-13 0.3477816549007713 1106 14-15 0.1273829912781963 1106 16-17 0.0639123101108865 1106 18-19 0.03852044108080577 1106 20-21 0.23958178834016053 1106 22-23 0.17747397435699241 1106 24-25 0.06349049818264518 1106 26-27 0.17989815153465116 1106 28-29 0.3260513444672455 1106 30-31 0.277451386410803 1106 32-33 0.36214625914026044 1106 34-35 0.5089183934472388 1106 36-37 0.09289417568420788 1106 38-39 0.11020513301290435 1106 40-41 -0.1685891930863619 1106 42-43 0.022893108913699223 1106 44-45 0.04991217583699381 1106 46-47 -0.020227013185042608 1106 48-49 -0.14032812756119029 1106 50-51 -0.30946272564715116 1106 52-53 -0.4104211668721476 1106 54-55 -0.14502664153926048 1106 56-57 -0.3731850742578544 1106 58-59 -0.5577869829804953 1106 60-61 -0.5935756777448233 1106 62-63 -0.3864675530298669 1106 64-65 -0.7557234794545806 1106 66-67 -0.4923563290555819 1106 68-69 -0.7504150407214922 1106 70-71 -0.4531045052853315 1106 72-73 -0.3270658553893462 1106 74-75 -0.2233326462890055 1106 76-77 -0.369924113368608 1106 78-79 -0.28925269553058186 1106 80-81 -0.200133181829802 1106 82-83 -0.23408957116730633 1106 84-85 -0.013264719117319856 1106 86-87 -0.0772763977028319 1106 88-89 0.29602428213795307 1106 90-91 0.08486256821903382 1106 92-93 -0.13097051690845873 1106 94-95 -0.13730739634859646 1106 96-97 -0.09438355784500985 1106 98-99 -0.0010542457231323965 1106 100-101 0.3664543165152203 1107 1 0.04876167103729401 1107 2 -0.3149013349562111 1107 3 -0.07008057888159414 1107 4 -0.0051308825934270885 1107 5 0.013164842424203016 1107 6 0.062136288274409424 1107 7 0.01627339840130304 1107 8 0.12296670602430737 1107 9 -0.07624206914181286 1107 10-11 -0.096412293305292 1107 12-13 0.1826814884041852 1107 14-15 0.1905233914667761 1107 16-17 -0.031083244012435784 1107 18-19 0.10410458153470614 1107 20-21 0.09074338551390326 1107 22-23 0.2905066590100702 1107 24-25 0.04563836386346054 1107 26-27 0.2284626406168897 1107 28-29 0.01356290673040661 1107 30-31 0.18201124462015628 1107 32-33 0.4470207302599647 1107 34-35 0.3353412231731454 1107 36-37 0.226380876843713 1107 38-39 0.15254837856254966 1107 40-41 0.37746889572375153 1107 42-43 0.31918841060670644 1107 44-45 0.5145689481902096 1107 46-47 0.5406367400896883 1107 48-49 0.5142391901727805 1107 50-51 0.49762501626523203 1107 52-53 0.5293237770620678 1107 54-55 0.7152773490080193 1107 56-57 0.42886599663150093 1107 58-59 0.6703222960713688 1107 60-61 0.5799328649027728 1107 62-63 0.4753253586314585 1107 64-65 0.4164065430433439 1107 66-67 0.42794312610074314 1107 68-69 0.4175913565668239 1107 70-71 0.5731977785227187 1107 72-73 0.5929799759299108 1107 74-75 0.6609760944835621 1107 76-77 0.5102490669549589 1107 78-79 0.6111178936525121 1107 80-81 0.5571010651461066 1107 82-83 0.5999151193817767 1107 84-85 0.7591481685541766 1107 86-87 0.9732424868296938 1107 88-89 0.9394381186609806 1107 90-91 0.46973524905282815 1107 92-93 0.7909073736573298 1107 94-95 0.8483882192412153 1107 96-97 0.5236201540144414 1107 98-99 0.5095173925675027 1107 100-101 0.712868672221159 1108 1 -0.02301965662758576 1108 2 -0.15854470182612346 1108 3 -0.09918068516510914 1108 4 -0.18036682400164494 1108 5 -0.07613728244694329 1108 6 0.08743256355670326 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2316 64-65 -0.14152698029557342 2316 66-67 -0.3151415085811209 2316 68-69 -0.252081097208233 2316 70-71 -0.12479056506174402 2316 72-73 -0.8266201587495807 2316 74-75 -0.9292710234226433 2316 76-77 -0.36646465849804244 2316 78-79 -0.4938752063944385 2316 80-81 -0.8679644962634754 2316 82-83 -0.7559446242529582 2316 84-85 -0.851784581800306 2316 86-87 -1.2998249335363106 2316 88-89 -0.8746828368046842 2316 90-91 -0.7645264559706213 2316 92-93 -0.6960846736132744 2316 94-95 -0.868687623335525 2316 96-97 -1.1235077108887452 2316 98-99 -1.293717760677847 2316 100-101 -1.468348754546863 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2190.0 3 437.0 4 432.0 5 451.0 6 509.0 7 605.0 8 724.0 9 896.0 10 1071.0 11 1413.0 12 1836.0 13 2364.0 14 2738.0 15 3407.0 16 4286.0 17 5742.0 18 4863.0 19 3423.0 20 3336.0 21 3478.0 22 3730.0 23 4100.0 24 4592.0 25 5122.0 26 5987.0 27 6925.0 28 8149.0 29 9851.0 30 12120.0 31 14991.0 32 19041.0 33 24940.0 34 34474.0 35 49528.0 36 71795.0 37 99709.0 38 79250.0 39 10916.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.09188261324892 19.10822132457736 14.560547720848197 22.23934834132552 2 15.341196475559379 20.271437821412334 40.63616271311864 23.751202989909643 3 19.416174470622735 24.763381795208396 29.624264658126304 26.196179076042565 4 12.466172131938034 16.271819589952113 36.918677313137316 34.34333096497254 5 13.356922586447462 37.90089758551268 33.85595730318947 14.886222524850387 6 31.804082852922487 36.319059673222284 17.279842020066127 14.5970154537891 7 28.633446175211112 33.30120519846336 19.859908218442467 18.205440407883064 8 26.986065870430693 35.02041731577208 19.197981085461613 18.795535728335615 9 26.689063597185697 16.626770495060022 19.78922232952992 36.89494357822436 10-11 25.055277311122737 27.252107816329822 26.574321426744774 21.118293445802664 12-13 26.10943741031111 24.411213041293035 27.538656587086376 21.94069296130948 14-15 22.854765761220712 25.750186606244597 26.023187362503897 25.371860270030794 16-17 21.800191792274216 29.370970518085027 26.622144631045757 22.206693058594997 18-19 21.8204025277468 27.22716875443762 29.789039743370367 21.163388974445212 20-21 22.4806384516662 26.290991163758132 30.065939901753975 21.162430482821698 22-23 22.071000912638468 26.385184573262837 30.0384921009567 21.505322413141993 24-25 21.818078117117658 26.662071040771597 30.07879761119235 21.4410532309184 26-27 21.386836978254394 27.137606793056275 29.7957421447419 21.67981408394743 28-29 21.46392650863913 27.246326071199917 29.916396791194572 21.373350628966385 30-31 21.52440655562287 26.9602154260132 29.832431135274636 21.682946883089294 32-33 21.335215118537715 27.031614974945047 29.805805667976376 21.827364238540863 34-35 21.89097438983854 27.112725227218636 29.609917569935746 21.386382813007074 36-37 21.794232563994388 26.824291166714364 29.637595429794256 21.743880839496992 38-39 21.473293965513 27.222798589838774 29.75243508488993 21.551472359758286 40-41 21.71204534458819 27.14086795080065 29.742537298755856 21.404549405855306 42-43 21.528115123420978 27.190572372031003 29.367131925036826 21.914180579511193 44-45 21.604857530342354 27.525906926839056 29.329385049168334 21.53985049365026 46-47 21.846644632227775 27.753657464452996 28.59091369495119 21.80878420836804 48-49 21.91304330722987 27.91933134746389 28.34613920820258 21.821486137103665 50-51 21.937433736764564 28.215401985487492 28.16848909004719 21.678675187700758 52-53 22.20551309426276 28.062155333255305 28.125101426810907 21.607230145671032 54-55 21.85139505240298 28.239385641897208 28.111230447235076 21.797988858464734 56-57 21.766419754543193 28.63295642605764 27.84069398924836 21.759929830150814 58-59 21.808553522935313 28.088701161562827 28.050159378140123 22.052585937361734 60-61 21.67201566943163 28.15689490389494 28.50418677459066 21.66690265208277 62-63 21.75172103203907 28.22374547310313 28.49690799300279 21.527625501855006 64-65 21.58086630246251 28.27144615796077 28.38963552052483 21.758052019051892 66-67 21.352737671676078 28.443896365964328 28.28793001378719 21.915435948572405 68-69 21.634826693464095 28.179285810505867 28.4041600254864 21.78172747054364 70-71 21.45717218837393 28.419420309236585 28.14054885363772 21.982858648751765 72-73 21.540957972532418 28.382874310974742 28.062665034150086 22.013502682342757 74-75 21.4260639380581 28.556776960193837 27.94937425820072 22.06778484354734 76-77 21.633964368212837 28.478780136906153 27.909304838917453 21.97795065596356 78-79 21.62330931912542 28.434513589366876 27.817735898782374 22.124441192725328 80-81 21.608416225166867 28.552155974308448 27.869060401892646 21.97036739863204 82-83 21.5688626994367 28.62536189696951 27.811683764987254 21.994091638606537 84-85 21.540789597885595 28.590475740985465 27.65903262853187 22.209702032597068 86-87 21.752272396527786 28.546059540525555 27.620778219605345 22.080889843341318 88-89 21.657631222091 28.41282656174865 27.48443586079941 22.445106355360938 90-91 21.429863808446825 28.44859629283642 27.86892177589852 22.252618122818234 92-93 21.591179519050925 28.143997576963848 27.914576175182365 22.35024672880286 94-95 21.22594520388987 28.135297871859194 28.043154813122666 22.595602111128265 96-97 21.4260991323364 27.832296279980806 28.12738450162441 22.614220086058385 98-99 21.257206803675015 28.362576837335446 27.68327136522896 22.696944993760575 100-101 21.250750955963255 28.17183642286367 27.96043801749592 22.616974603677154 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 875.0 1 517.0 2 196.0 3 238.0 4 216.0 5 221.5 6 374.5 7 709.0 8 1079.5 9 1222.5 10 1077.5 11 920.0 12 924.5 13 945.0 14 977.0 15 1037.5 16 1091.5 17 1131.5 18 1167.5 19 1236.5 20 1350.0 21 1525.0 22 1793.0 23 2069.0 24 2419.5 25 2891.0 26 3531.0 27 4335.0 28 4993.0 29 5728.0 30 6526.0 31 7472.5 32 8884.0 33 9638.0 34 10057.5 35 10863.5 36 11782.5 37 12867.0 38 13840.0 39 14281.0 40 14607.0 41 15537.0 42 15961.0 43 16335.0 44 17272.0 45 17996.0 46 18215.5 47 18307.0 48 18864.5 49 18822.0 50 18336.5 51 17881.0 52 17203.0 53 16982.5 54 16639.5 55 15353.0 56 14039.5 57 12655.0 58 11331.0 59 10007.0 60 8125.0 61 6618.0 62 5386.5 63 3956.0 64 2801.5 65 2052.0 66 1471.5 67 1126.5 68 839.0 69 599.0 70 444.5 71 299.0 72 212.5 73 165.0 74 127.0 75 90.0 76 56.5 77 40.5 78 28.5 79 17.5 80 11.5 81 8.0 82 6.5 83 5.0 84 4.5 85 4.5 86 4.5 87 2.5 88 1.0 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27972933977986775 2 0.2575472938885519 3 0.259902909381435 4 0.2618659222921709 5 0.251265652574197 6 0.19669389365573856 7 0.20415334271653504 8 0.20199402851472553 9 0.19983471431291602 10-11 0.21102388790411075 12-13 0.2093553269299852 14-15 0.19581053784590743 16-17 0.20778491660139647 18-19 0.19316047041641393 20-21 0.19698834559234896 22-23 0.19728279752895933 24-25 0.1897251978226261 26-27 0.2001291662495264 28-29 0.19777355075664332 30-31 0.2027792336790199 32-33 0.20385889077992464 34-35 0.19433827816285548 36-37 0.19581053784590743 38-39 0.19011780040477325 40-41 0.18825293813957414 42-43 0.18805663684850055 44-45 0.19914765979415847 46-47 0.19129560815121482 48-49 0.19541793526376022 50-51 0.2025829323879463 52-53 0.20601820498173415 54-55 0.20670525950049176 56-57 0.1846213642547127 58-59 0.1731377387269076 60-61 0.17961568133233613 62-63 0.18305095392612397 64-65 0.17961568133233613 66-67 0.19198266266997238 68-69 0.17951753068679932 70-71 0.18511211748239667 72-73 0.19698834559234896 74-75 0.18540656941900707 76-77 0.19051040298692046 78-79 0.19080485492353083 80-81 0.18197129682521923 82-83 0.1941419768717819 84-85 0.17843787358589458 86-87 0.18197129682521923 88-89 0.1885473900761845 90-91 0.18570102135561745 92-93 0.19001964975923646 94-95 0.1909030055690676 96-97 0.1823638994073664 98-99 0.1885473900761845 100-101 0.17716191519391622 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 509421.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.52479489673563 #Duplication Level Percentage of deduplicated Percentage of total 1 86.45018114604663 62.697816564026745 2 8.485862625118713 12.308708928172187 3 2.227885636082936 4.8473084643089495 4 0.9213089052468608 2.6727095755825845 5 0.5133068344510473 1.8613736443827418 6 0.3288726102953512 1.4310851165294647 7 0.20445084900651847 1.037942912346285 8 0.15876138152063157 0.9211309305844958 9 0.12047676264184873 0.7863797250380502 >10 0.5485503133925458 6.780083786016642 >50 0.024638976124073626 1.2471246682495183 >100 0.014350170411482751 1.8360280378250282 >500 5.415158645842038E-4 0.25625845875524345 >1k 8.122737968763058E-4 1.3160491881821008 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2893 0.5678996350758999 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2256 0.44285571266202217 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1553 0.30485590503728743 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 705 0.13839241020688192 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 600 0.11778077464415485 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 9.815064553679569E-5 0.0 18-19 0.0 0.0 0.0 1.9630129107359138E-4 1.9630129107359138E-4 20-21 0.0 0.0 0.0 2.9445193661038706E-4 1.9630129107359138E-4 22-23 0.0 0.0 0.0 8.833558098311613E-4 1.9630129107359138E-4 24-25 0.0 0.0 0.0 0.001275958391978344 1.9630129107359138E-4 26-27 0.0 0.0 0.0 0.0014722596830519354 1.9630129107359138E-4 28-29 0.0 0.0 0.0 0.002846368720567075 1.9630129107359138E-4 30-31 0.0 0.0 0.0 0.005594586795597354 1.9630129107359138E-4 32-33 0.0 0.0 0.0 0.010796571009047526 1.9630129107359138E-4 34-35 0.0 0.0 0.0 0.015802253931424107 1.9630129107359138E-4 36-37 0.0 0.0 0.0 0.0205134849171903 1.9630129107359138E-4 38-39 0.0 0.0 0.0 0.02689327687708202 1.9630129107359138E-4 40-41 0.0 0.0 0.0 0.03219341173606899 1.9630129107359138E-4 42-43 0.0 0.0 0.0 0.04622895404783077 1.9630129107359138E-4 44-45 0.0 0.0 0.0 0.06487757669982196 1.9630129107359138E-4 46-47 0.0 0.0 0.0 0.08185763837768761 2.9445193661038706E-4 48-49 0.0 0.0 0.0 0.10659160105296012 3.9260258214718276E-4 50-51 0.0 0.0 0.0 0.13986466988993387 3.9260258214718276E-4 52-53 0.0 0.0 0.0 0.19738094817449614 9.81506455367957E-4 54-55 0.0 0.0 0.0 0.23703380897136161 9.81506455367957E-4 56-57 0.0 0.0 0.0 0.3150635721731142 9.81506455367957E-4 58-59 0.0 0.0 0.0 0.40074908572673684 9.81506455367957E-4 60-61 9.815064553679569E-5 0.0 0.0 0.49310884317686154 9.81506455367957E-4 62-63 1.9630129107359138E-4 0.0 0.0 0.6350346766230681 9.81506455367957E-4 64-65 1.9630129107359138E-4 0.0 0.0 0.7095310165854961 9.81506455367957E-4 66-67 1.9630129107359138E-4 0.0 0.0 0.8703017739747674 9.81506455367957E-4 68-69 1.9630129107359138E-4 0.0 0.0 1.077105184120796 9.81506455367957E-4 70-71 1.9630129107359138E-4 0.0 0.0 1.2396426531297298 9.81506455367957E-4 72-73 1.9630129107359138E-4 0.0 0.0 1.515249665797052 9.81506455367957E-4 74-75 1.9630129107359138E-4 0.0 0.0 1.7854583929598504 9.81506455367957E-4 76-77 1.9630129107359138E-4 0.0 0.0 1.9623258562171562 9.81506455367957E-4 78-79 1.9630129107359138E-4 0.0 0.0 2.1636328302131242 9.81506455367957E-4 80-81 1.9630129107359138E-4 0.0 0.0 2.514423237361632 9.81506455367957E-4 82-83 1.9630129107359138E-4 0.0 0.0 2.812114145274734 9.81506455367957E-4 84-85 2.9445193661038706E-4 0.0 0.0 3.261349649896647 9.81506455367957E-4 86-87 3.9260258214718276E-4 0.0 0.0 3.8131525791045124 9.81506455367957E-4 88-89 3.9260258214718276E-4 0.0 0.0 4.55418995290732 9.81506455367957E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1395 0.0 50.782917 1 GCCGTTC 45 9.6216676E-4 42.220737 8 GTATCAA 2490 0.0 40.86204 1 TGCACCG 75 6.88838E-6 38.021095 5 ATCAACG 3155 0.0 32.092262 3 TCAACGC 3180 0.0 31.839968 4 CAACGCA 3245 0.0 31.049585 5 TATCAAC 3265 0.0 30.865461 2 AACGCAG 3310 0.0 30.421888 6 ACGCAGA 3750 0.0 26.725725 7 CTAATAC 180 6.6265784E-9 26.408737 3 CGCAGAG 3795 0.0 26.28366 8 ATAGCAC 110 9.41817E-5 25.928576 3 GGGCGCG 95 0.0012258651 24.99912 7 GTATAGA 115 1.2721577E-4 24.80613 1 GTACATG 2685 0.0 24.790733 1 GCAGAGT 3995 0.0 24.130825 9 GAGTACT 2275 0.0 23.799019 12-13 ACCCGGG 140 1.7774348E-5 23.749163 7 TACATGG 2710 0.0 23.680159 2 >>END_MODULE