FastQCFastQC Report
Thu 26 May 2016
SRR937209_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937209_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509421
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46510.9129973047832736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40620.7973758443409282No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28910.5675070324937528No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10420.20454594529868225No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8290.16273377030000727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16800.056.0145571
GTATCAA30500.042.6968351
TCCGTGC459.6205075E-442.2217868
ATCAACG40550.032.4504433
TATCAAC40650.032.2537542
TCAACGC40750.032.0580334
GTAAGGT1351.19343895E-831.6694494
CAACGCA41550.031.5551195
AACGCAG42400.030.8074656
TGGTATC7250.030.1405772
GTGGTAT7700.029.6275331
AGGACCG1205.3702934E-627.7107665
ACGCAGA47750.027.2562647
CGCAGAG47650.027.213788
ATGGGCG700.00838366827.145245
ACGCTCT700.00838768427.1425789
ATGCGCC458.947384E-426.38861782-83
CTATGCA1309.999971E-625.5791684
AGGGACG950.001224989725.0021955
GTACATG23200.024.992911
Produced by FastQC (version 0.11.5)