##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937209_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 509421 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16494608585041 33.0 31.0 34.0 31.0 34.0 2 32.380392641842406 34.0 31.0 34.0 31.0 34.0 3 32.446811183677156 34.0 31.0 34.0 31.0 34.0 4 36.076602652815644 37.0 35.0 37.0 35.0 37.0 5 35.966848637963494 37.0 35.0 37.0 35.0 37.0 6 36.058491895701195 37.0 35.0 37.0 35.0 37.0 7 36.01035293009122 37.0 35.0 37.0 35.0 37.0 8 35.98874800999566 37.0 35.0 37.0 35.0 37.0 9 37.68865830030564 39.0 37.0 39.0 35.0 39.0 10-11 37.623850410564145 39.0 37.0 39.0 35.0 39.0 12-13 37.538342549678944 39.0 37.0 39.0 35.0 39.0 14-15 38.729049253956944 40.0 38.0 41.0 34.5 41.0 16-17 38.706488346573856 40.0 38.0 41.0 34.5 41.0 18-19 38.738056538697855 40.0 38.0 41.0 35.0 41.0 20-21 38.65008509660968 40.0 38.0 41.0 34.0 41.0 22-23 38.531283555251946 40.0 38.0 41.0 34.0 41.0 24-25 38.39725394123917 40.0 38.0 41.0 34.0 41.0 26-27 38.31849393723462 40.0 38.0 41.0 34.0 41.0 28-29 38.216443766550654 40.0 38.0 41.0 34.0 41.0 30-31 38.21691881567505 40.0 37.5 41.0 34.0 41.0 32-33 38.0885603459614 40.0 37.0 41.0 34.0 41.0 34-35 37.94784863600048 40.0 37.0 41.0 33.0 41.0 36-37 37.76970815887056 40.0 37.0 41.0 33.0 41.0 38-39 37.624283254910964 40.0 37.0 41.0 33.0 41.0 40-41 37.423169637686705 40.0 36.0 41.0 33.0 41.0 42-43 37.19275707126326 40.0 36.0 41.0 32.5 41.0 44-45 36.832951527322194 40.0 36.0 41.0 31.0 41.0 46-47 36.37596702923515 39.0 35.5 41.0 30.5 41.0 48-49 35.97775317468263 39.0 35.0 41.0 29.5 41.0 50-51 35.71538079505949 39.0 35.0 41.0 28.0 41.0 52-53 35.47228912824559 39.0 35.0 40.5 27.5 41.0 54-55 35.23429246144152 39.0 35.0 40.0 27.0 41.0 56-57 34.980965645311045 38.0 35.0 40.0 26.5 41.0 58-59 34.720505240262966 38.0 34.0 40.0 26.0 41.0 60-61 34.385603459613954 38.0 34.0 40.0 26.0 41.0 62-63 34.01154546043449 37.0 34.0 40.0 25.0 41.0 64-65 33.63904020446742 36.5 33.5 39.5 23.5 41.0 66-67 33.215707636709126 36.0 33.0 39.0 22.5 40.5 68-69 32.79949786129743 35.5 32.5 39.0 21.5 40.0 70-71 32.3788094719299 35.0 32.0 38.0 20.0 40.0 72-73 31.94859458090656 35.0 32.0 37.5 18.5 40.0 74-75 31.51966840785912 35.0 31.0 37.0 18.0 39.0 76-77 30.19493012655544 33.5 29.5 35.5 14.5 38.0 78-79 30.843533148417517 34.0 31.0 36.0 15.0 38.5 80-81 30.70662477597115 34.5 31.0 36.0 12.0 37.5 82-83 30.391070450570354 34.0 31.0 35.0 7.5 37.0 84-85 30.036681840756465 34.0 31.0 35.0 4.5 36.5 86-87 29.65985403035996 34.0 30.0 35.0 2.0 36.0 88-89 29.254254339730792 34.0 30.0 35.0 2.0 36.0 90-91 28.834651496502893 34.0 29.0 35.0 2.0 35.0 92-93 28.485060490242844 34.0 29.0 35.0 2.0 35.0 94-95 28.076955995139578 34.0 29.0 35.0 2.0 35.0 96-97 27.60664067637573 33.0 27.5 35.0 2.0 35.0 98-99 27.13959671862762 33.0 27.0 35.0 2.0 35.0 100-101 25.255701080246006 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08916970869554319 1101 2 0.15552802632963392 1101 3 0.062395795877677074 1101 4 0.18333897592859927 1101 5 0.11558058730648924 1101 6 0.02104855821709606 1101 7 0.011636878177505139 1101 8 0.10766925752815126 1101 9 0.1735311427347881 1101 10-11 0.19853179402203125 1101 12-13 0.2174785313612091 1101 14-15 0.2470413609346167 1101 16-17 0.29092746387626534 1101 18-19 0.12120192831318377 1101 20-21 0.12272270244133665 1101 22-23 0.10051797308727828 1101 24-25 0.001144124003630509 1101 26-27 0.012117243461887028 1101 28-29 0.20551229011832817 1101 30-31 0.22145769748950528 1101 32-33 0.3227265720062249 1101 34-35 0.11379923000779257 1101 36-37 0.18901532379911856 1101 38-39 0.19157953277684925 1101 40-41 -0.13122819736028646 1101 42-43 -0.0029833813504467344 1101 44-45 0.006094363656764301 1101 46-47 0.31188060853562405 1101 48-49 0.45019793754422466 1101 50-51 0.30238450791451754 1101 52-53 0.2353540881740841 1101 54-55 0.44585386351143796 1101 56-57 0.4102043056539344 1101 58-59 0.2337761248240966 1101 60-61 0.4424670310394845 1101 62-63 0.41283479874645934 1101 64-65 0.33653081795981166 1101 66-67 0.35913419218113773 1101 68-69 0.31421815607418324 1101 70-71 0.2048414038084374 1101 72-73 0.004345695513762848 1101 74-75 -0.15062148717126078 1101 76-77 0.0973782572464934 1101 78-79 0.0911192468805524 1101 80-81 -0.1222179434856443 1101 82-83 0.08205672042839396 1101 84-85 0.1429164464816175 1101 86-87 0.10445387744852397 1101 88-89 -0.060012611951282935 1101 90-91 0.021901600489311335 1101 92-93 -0.21797489466291609 1101 94-95 -0.22153024323185022 1101 96-97 0.025020458250299527 1101 98-99 0.13465852140724976 1101 100-101 0.14740844333518055 1102 1 0.049875760211520515 1102 2 0.10430127834804637 1102 3 0.12862118034685466 1102 4 0.17526532973114684 1102 5 -0.05410646593660573 1102 6 0.12517132391607788 1102 7 0.05055069592960848 1102 8 0.032973568706474055 1102 9 0.007785879907437732 1102 10-11 -0.11500581826114598 1102 12-13 0.06977295087678925 1102 14-15 0.014149309798767717 1102 16-17 0.03590932502938671 1102 18-19 -0.01864073061864957 1102 20-21 0.07730534368882758 1102 22-23 0.23171265786800177 1102 24-25 0.05770096594362428 1102 26-27 0.04178809983032039 1102 28-29 0.012951283267717884 1102 30-31 0.05174172125594367 1102 32-33 0.029749074092812577 1102 34-35 0.10639907630275758 1102 36-37 -0.0821996897120485 1102 38-39 -0.1101285764033122 1102 40-41 -0.16867933816565994 1102 42-43 0.03692208178927103 1102 44-45 0.04533459801562145 1102 46-47 0.1537710151928664 1102 48-49 -0.3715496721772027 1102 50-51 -0.13067116471028584 1102 52-53 0.06773709467762501 1102 54-55 -0.14176627019541854 1102 56-57 -0.149670416478763 1102 58-59 -0.49557492590682983 1102 60-61 -0.46334731641653804 1102 62-63 -0.6260046456015687 1102 64-65 -0.5889305037382115 1102 66-67 -0.31500286973885494 1102 68-69 -0.28082692247239294 1102 70-71 -0.27653632218341784 1102 72-73 -0.5744901128011826 1102 74-75 -0.5066469724577196 1102 76-77 -0.35127930281003117 1102 78-79 -0.3890767683577323 1102 80-81 -0.5602938208852173 1102 82-83 -0.3976930543854671 1102 84-85 -0.3401759996596425 1102 86-87 -0.29450158940986526 1102 88-89 -0.5146076487661198 1102 90-91 -0.4820484536379972 1102 92-93 -0.3977932582827499 1102 94-95 -0.5580203143824072 1102 96-97 -0.5615460522322167 1102 98-99 -0.5244964314482807 1102 100-101 -0.23447006357741174 1103 1 0.173336476122806 1103 2 0.27021661850255185 1103 3 0.17796244469532496 1103 4 0.18648846435264232 1103 5 0.05006604611267562 1103 6 0.007546638007973172 1103 7 0.16385447500844208 1103 8 0.08006085351845371 1103 9 0.15079667282189746 1103 10-11 -0.06631456045951012 1103 12-13 0.21112053759109273 1103 14-15 0.23887543413863455 1103 16-17 0.17003201883109398 1103 18-19 0.24694539758695555 1103 20-21 0.1594384251251384 1103 22-23 0.15576788816049003 1103 24-25 0.16188750691118514 1103 26-27 0.07512860349966388 1103 28-29 0.12740699241394537 1103 30-31 0.23289402542783222 1103 32-33 0.2942300352919389 1103 34-35 0.14157892703506292 1103 36-37 0.01560057590213404 1103 38-39 0.024224815165624136 1103 40-41 0.07503882398368944 1103 42-43 0.016634707192210385 1103 44-45 -0.1993032376652124 1103 46-47 -0.015052987351047875 1103 48-49 -0.011004913692822527 1103 50-51 -0.27039919074795904 1103 52-53 0.03660380748384284 1103 54-55 -0.12531190782534196 1103 56-57 -0.1121040908706874 1103 58-59 -0.1303928305475921 1103 60-61 -0.21113386486617713 1103 62-63 -0.1743202401471322 1103 64-65 0.056240742590887294 1103 66-67 0.012723572589820265 1103 68-69 0.08197649185455447 1103 70-71 0.04876467605205903 1103 72-73 0.1695023926389183 1103 74-75 0.18011222271882588 1103 76-77 0.2055721091226559 1103 78-79 0.32564673725071813 1103 80-81 0.301362339680427 1103 82-83 0.09805462460794345 1103 84-85 0.14008219478740003 1103 86-87 0.16369535166244376 1103 88-89 0.30448165621009693 1103 90-91 0.006522654272441031 1103 92-93 0.016066065959844167 1103 94-95 0.1217524794922582 1103 96-97 0.17892363595169414 1103 98-99 0.2670478718756435 1103 100-101 0.6050889919334566 1104 1 -0.1088181324268902 1104 2 3.110674614816844E-4 1104 3 -0.0831576213789802 1104 4 -0.07343933348128928 1104 5 -0.08987560601142519 1104 6 -0.19188187252262168 1104 7 -0.22321307797756162 1104 8 -0.2490784487048927 1104 9 -0.11491262687037818 1104 10-11 -0.1653694047985752 1104 12-13 -0.10429768879333778 1104 14-15 -0.24209156867219406 1104 16-17 -0.21809458806674087 1104 18-19 -0.23757176707928096 1104 20-21 -0.32051281843062185 1104 22-23 -0.3956095479965427 1104 24-25 -0.2604085752554184 1104 26-27 -0.20083819948966664 1104 28-29 -0.2513946114865675 1104 30-31 -0.3862095753554726 1104 32-33 -0.42140732866292296 1104 34-35 -0.4550314343754067 1104 36-37 -0.5689738709253618 1104 38-39 -0.5268462280357795 1104 40-41 -0.6038280549378072 1104 42-43 -0.5308528705716569 1104 44-45 -0.30153524373978513 1104 46-47 -0.7946831843750388 1104 48-49 -0.7579742877186959 1104 50-51 -0.9695379601139891 1104 52-53 -1.1954488921045225 1104 54-55 -1.2476126524934372 1104 56-57 -1.1389667602194038 1104 58-59 -1.4746153251191103 1104 60-61 -1.4400726795250947 1104 62-63 -1.28533107047204 1104 64-65 -1.4835636987748302 1104 66-67 -1.2414060715947102 1104 68-69 -1.084388222334102 1104 70-71 -1.1911843028601155 1104 72-73 -0.9853492732062605 1104 74-75 -1.1968543538061454 1104 76-77 -1.0343169233416774 1104 78-79 -0.9925157043014963 1104 80-81 -1.1079153722302664 1104 82-83 -1.1565612518757327 1104 84-85 -1.1857234188644021 1104 86-87 -1.1048247703618799 1104 88-89 -1.2595011857843907 1104 90-91 -1.3445037621289053 1104 92-93 -1.5230856251960567 1104 94-95 -1.4978739875305358 1104 96-97 -1.5965536043802793 1104 98-99 -1.638070222626606 1104 100-101 -1.1502795455064287 1105 1 -0.06075054648786704 1105 2 -0.020708018327461275 1105 3 -0.16248458689744183 1105 4 -0.07104698898368156 1105 5 0.006330188582403196 1105 6 0.039389866032088605 1105 7 -0.1422706178352584 1105 8 -0.0039734856935425 1105 9 0.06507819217657129 1105 10-11 -0.15433901937510086 1105 12-13 -0.22311158028153244 1105 14-15 -0.2027194046156211 1105 16-17 -0.1707950241620111 1105 18-19 -0.05144559959750694 1105 20-21 -0.08979723376281612 1105 22-23 -0.12037516885849442 1105 24-25 -0.03872828428983155 1105 26-27 -0.1820613849272661 1105 28-29 -0.35908101131708037 1105 30-31 -0.12383735833186194 1105 32-33 0.02129116530766595 1105 34-35 -0.27256440635584767 1105 36-37 -0.3839511833779099 1105 38-39 -0.5029978196952385 1105 40-41 -0.24526328507957373 1105 42-43 -0.5484379268049864 1105 44-45 -0.4943043604261632 1105 46-47 -0.5334144472857574 1105 48-49 -0.26112582641111715 1105 50-51 -0.018987102930779542 1105 52-53 -0.08282773000773602 1105 54-55 -0.17495953361775207 1105 56-57 -0.01324067042261845 1105 58-59 -0.05131159447415001 1105 60-61 -0.1731629530115555 1105 62-63 -0.3224124101849597 1105 64-65 -0.240422930046563 1105 66-67 -0.21024379824948625 1105 68-69 0.027535540444198148 1105 70-71 0.12110934639216708 1105 72-73 -0.14677073191845125 1105 74-75 -0.23834078541687376 1105 76-77 -0.1613936725780647 1105 78-79 -0.03687118374145726 1105 80-81 -0.2844986452400313 1105 82-83 -0.05350946776937704 1105 84-85 0.16883351187236784 1105 86-87 -0.041877860988481785 1105 88-89 -0.1405548767040763 1105 90-91 0.17939761324848646 1105 92-93 -0.32283932847471775 1105 94-95 0.05199447766129239 1105 96-97 0.27581790641616877 1105 98-99 0.32445118488412206 1105 100-101 0.5971692088854219 1106 1 -0.1401739111009519 1106 2 0.12632535086012808 1106 3 -0.02100862779602153 1106 4 0.13327901277194343 1106 5 0.1614616748032418 1106 6 -0.19488062344036194 1106 7 0.04692241407503417 1106 8 0.03985525458562478 1106 9 -0.23688884663257426 1106 10-11 -0.06940460794130132 1106 12-13 -0.26729577226254264 1106 14-15 -0.2897092541513686 1106 16-17 0.07057213580980459 1106 18-19 -0.08899057196500593 1106 20-21 0.018114812031733152 1106 22-23 0.1710828631136181 1106 24-25 0.2368858744332698 1106 26-27 0.24699354626964976 1106 28-29 0.212064964969656 1106 30-31 -0.0545830032758019 1106 32-33 -0.07822912943907312 1106 34-35 0.05327353239518828 1106 36-37 -0.2106422062133717 1106 38-39 -0.20233513408763315 1106 40-41 -0.1891637338759864 1106 42-43 -0.28973708732079473 1106 44-45 -0.7352480692401357 1106 46-47 -0.5827635070387274 1106 48-49 -0.49155743750367975 1106 50-51 -0.4784841542270968 1106 52-53 -0.1787316900487781 1106 54-55 -0.36463783437316266 1106 56-57 -0.160434765597131 1106 58-59 -0.4806199781888907 1106 60-61 -0.4821648446327842 1106 62-63 -0.2782251273195868 1106 64-65 -0.4237031001688436 1106 66-67 -0.2665164389774546 1106 68-69 0.020534687360111548 1106 70-71 -0.20494804514870424 1106 72-73 -0.4213123537672985 1106 74-75 -0.2893651044950474 1106 76-77 -0.24981460157427193 1106 78-79 0.3025728279791764 1106 80-81 0.07615501498523258 1106 82-83 0.053954399792413454 1106 84-85 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2316 56-57 0.07227459244053591 2316 58-59 0.20472084709271599 2316 60-61 0.2837011598560153 2316 62-63 0.3024512703903781 2316 64-65 -0.034015671467038544 2316 66-67 -0.0767637475533931 2316 68-69 0.1178477733318104 2316 70-71 0.04037877252422817 2316 72-73 -0.02707245224502941 2316 74-75 0.1940300427174897 2316 76-77 -0.07759529760385675 2316 78-79 -0.06599441650134708 2316 80-81 0.18175063017924487 2316 82-83 0.15510100745733268 2316 84-85 0.19196360235899235 2316 86-87 0.1173162087481181 2316 88-89 -0.14443568194660017 2316 90-91 0.08452174989212935 2316 92-93 0.3463613238935572 2316 94-95 0.4331632386528135 2316 96-97 0.37174011346450087 2316 98-99 0.31693519498404044 2316 100-101 -0.2369632100843475 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 4.0 5 12.0 6 16.0 7 66.0 8 143.0 9 258.0 10 485.0 11 746.0 12 1178.0 13 1694.0 14 2300.0 15 3309.0 16 4501.0 17 6697.0 18 5977.0 19 3534.0 20 2780.0 21 2771.0 22 2830.0 23 2963.0 24 3325.0 25 3821.0 26 4568.0 27 5645.0 28 6889.0 29 8693.0 30 10950.0 31 13590.0 32 17402.0 33 23066.0 34 32619.0 35 47669.0 36 73115.0 37 112117.0 38 93871.0 39 9814.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.951258890008425 19.14840544527133 15.122196492509904 21.778139172210338 2 16.05273437883401 20.54626723279959 40.07216035459865 23.32883803376775 3 19.663696628132723 24.56475096236708 30.612204836471207 25.159347573028988 4 12.867549629873915 17.048963431032487 36.599786816797895 33.48370012229571 5 13.109334108181653 37.727129049547756 34.02160020575364 15.141936636516954 6 31.727588772351357 36.650825152477026 16.709362197475173 14.912223877696443 7 28.102492830095343 33.83664984364602 19.97777869385047 18.083078632408164 8 27.34143272460303 34.507411355244486 19.05791084387962 19.093245076272865 9 26.735058036476705 16.209971712983958 19.92202912718557 37.13294112335377 10-11 25.23089939362531 27.019793059178948 26.444630276333324 21.30467727086241 12-13 26.404664219391062 24.30193753558038 27.730904379576373 21.56249386545219 14-15 22.99041460795688 25.3138366891039 26.77991288148702 24.915835821452198 16-17 21.575671203189504 28.58745516969265 27.69870107435697 22.138172552760878 18-19 21.452393992395287 25.96742183773343 31.516564884447245 21.06361928542404 20-21 22.079969219957558 24.913087603377168 32.17162229275982 20.835320883905453 22-23 21.64400018845109 25.104531463479752 32.21718595411216 21.034282393957003 24-25 21.519746457003595 25.246039372188594 32.25625980893977 20.977954361868044 26-27 21.1735803091975 25.653322287994772 32.11298046486611 21.060116937941622 28-29 21.146316637727704 25.78014960576594 32.1368732767903 20.93666047971606 30-31 21.276900638175498 25.52996441057593 31.96570223842362 21.227432712824953 32-33 21.047326278264933 25.50189332595241 32.06866226559172 21.382118130190943 34-35 21.445916049789858 25.531731122195588 32.01448310925541 21.00786971875914 36-37 21.50343232807442 25.28733601480897 31.889537337487067 21.31969431962954 38-39 21.22723641153388 25.673853256932873 31.93449033314292 21.16441999839033 40-41 21.357187866224596 25.75198117078016 31.79963134617536 21.091199616819882 42-43 21.374167943607166 25.527357611726977 31.550557518275546 21.54791692639031 44-45 21.522964306536245 25.93915445181883 31.246748759866595 21.291132481778334 46-47 21.74449031351279 26.371213593471804 30.56803704597965 21.31625904703575 48-49 21.83557411257094 26.72661708096054 30.027914043590663 21.409894762877855 50-51 21.889164365034027 26.778244320512893 30.007596859964547 21.32499445448853 52-53 22.20334458139731 26.66811929622061 29.679773703871646 21.448762418510427 54-55 21.708076424018643 26.982299512583896 29.815221594712426 21.49440246868504 56-57 21.785124680765026 27.157007661639394 29.603118049707412 21.45474960788817 58-59 21.771874343617558 26.695208874388765 29.84859281419494 21.684323967798736 60-61 21.65213055606267 26.73044495613648 30.18181425579236 21.435610232008496 62-63 21.552900253424966 26.92026830460464 30.263377442233434 21.263453999736956 64-65 21.491065346736786 26.80346903641585 30.061285263073174 21.644180353774185 66-67 21.432567561996855 27.181937925605737 29.76909079131013 21.616403721087273 68-69 21.655958431238602 26.79708924445596 29.97756276243029 21.56938956187515 70-71 21.622881663692702 27.040502845387216 29.681736716782385 21.6548787741377 72-73 21.5616568237831 26.937667408359108 29.529337747499362 21.971338020358427 74-75 21.480759528955424 27.11539178793179 29.41123353768298 21.992615145429813 76-77 21.495285824494868 26.969049175436428 29.65739535668926 21.878269643379443 78-79 21.60354598652195 27.069555436466107 29.38149389208533 21.94540468492661 80-81 21.45141248593992 27.38412825541154 29.314162549247087 21.850296709401455 82-83 21.526953718889835 27.278598483175458 29.28922037731182 21.905227420622886 84-85 21.36219263703 27.247876972364327 29.212879897293753 22.177050493311924 86-87 21.53228861786224 27.163583754890357 29.120118723020845 22.184008904226562 88-89 21.199165326910354 27.132470000255193 29.157710420261434 22.51065425257302 90-91 21.21902809925143 26.852031811658645 29.459354932436543 22.469585156653377 92-93 21.179897804745423 26.52041451306101 29.724076738703264 22.575610943490304 94-95 20.9538868637139 26.55141817867736 29.728358273412365 22.76633668419637 96-97 20.989908150625908 26.3162492319712 29.750932921885827 22.942909695517066 98-99 20.79824035349971 26.523667579141396 29.71219257960761 22.96589948775128 100-101 20.4247687536995 26.783342609689225 29.72625424675499 23.065634389856278 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 255.0 1 200.5 2 236.0 3 357.0 4 285.0 5 210.5 6 460.0 7 1122.0 8 1794.0 9 2024.5 10 1656.5 11 1168.5 12 1032.0 13 1020.0 14 1097.5 15 1187.5 16 1239.0 17 1317.0 18 1388.5 19 1447.5 20 1581.5 21 1698.5 22 1876.0 23 2157.5 24 2478.0 25 2881.0 26 3530.5 27 4372.5 28 5002.0 29 5590.0 30 6374.5 31 7428.5 32 8658.5 33 9396.5 34 9831.5 35 10568.5 36 11614.5 37 12623.5 38 13449.5 39 13920.5 40 14335.0 41 15269.0 42 15839.0 43 16316.0 44 17016.5 45 17751.0 46 18233.0 47 18615.0 48 19190.0 49 18861.5 50 18300.0 51 17876.0 52 17234.5 53 17010.5 54 16711.5 55 15227.0 56 13635.5 57 12303.5 58 11182.5 59 10127.0 60 8319.5 61 6563.5 62 5308.5 63 3975.0 64 2698.5 65 1958.5 66 1424.5 67 1052.0 68 760.5 69 533.5 70 392.0 71 269.0 72 190.5 73 148.5 74 120.5 75 100.5 76 64.0 77 32.5 78 23.5 79 17.5 80 9.5 81 4.5 82 4.0 83 3.5 84 2.5 85 2.0 86 1.5 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05535696408275277 2 0.0 3 0.0 4 0.0 5 0.014526295539445762 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.002159314201809505 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.81506455367957E-4 24-25 9.815064553679569E-5 26-27 0.0010796571009047526 28-29 0.0030426700116406667 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.012857734565320234 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 9.815064553679569E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.944519366103871E-4 84-85 0.0013741090375151397 86-87 0.0 88-89 0.0 90-91 0.009422461971532388 92-93 7.852051642943655E-4 94-95 0.0 96-97 0.0 98-99 9.815064553679569E-5 100-101 0.01207252940102587 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 509421.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.85903183182648 #Duplication Level Percentage of deduplicated Percentage of total 1 85.07769473076742 58.58367689644329 2 9.16565872998717 12.622767724956896 3 2.521256816100652 5.208339100682529 4 1.063977204811434 2.930577608577932 5 0.5862437004201716 2.0184086814220175 6 0.3516299564468057 1.4527739018396613 7 0.22813632042532414 1.0996472305114213 8 0.17311316365180013 0.9536323877125996 9 0.13173194864122265 0.8163841000279034 >10 0.6508005510599587 7.739603615288015 >50 0.029453784699192975 1.439205303224437 >100 0.018873297708032054 2.481639558696827 >500 2.8595905618229473E-4 0.1632372605061997 >1k 0.001143836224729179 2.490106630110255 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4651 0.9129973047832736 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4062 0.7973758443409282 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2891 0.5675070324937528 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1042 0.20454594529868225 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 829 0.16273377030000727 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.9630129107359138E-4 2 0.0 0.0 0.0 0.0 1.9630129107359138E-4 3 0.0 0.0 0.0 0.0 1.9630129107359138E-4 4 0.0 0.0 0.0 0.0 1.9630129107359138E-4 5 0.0 0.0 0.0 0.0 1.9630129107359138E-4 6 0.0 0.0 0.0 0.0 1.9630129107359138E-4 7 0.0 0.0 0.0 0.0 1.9630129107359138E-4 8 0.0 0.0 0.0 0.0 1.9630129107359138E-4 9 0.0 0.0 0.0 0.0 1.9630129107359138E-4 10-11 0.0 0.0 0.0 0.0 1.9630129107359138E-4 12-13 1.9630129107359138E-4 0.0 0.0 0.0 1.9630129107359138E-4 14-15 1.9630129107359138E-4 0.0 0.0 0.0 1.9630129107359138E-4 16-17 1.9630129107359138E-4 0.0 0.0 9.815064553679569E-5 1.9630129107359138E-4 18-19 1.9630129107359138E-4 0.0 0.0 1.9630129107359138E-4 1.9630129107359138E-4 20-21 1.9630129107359138E-4 0.0 0.0 2.9445193661038706E-4 1.9630129107359138E-4 22-23 1.9630129107359138E-4 0.0 0.0 6.870545187575699E-4 1.9630129107359138E-4 24-25 1.9630129107359138E-4 0.0 0.0 0.0010796571009047528 1.9630129107359138E-4 26-27 1.9630129107359138E-4 0.0 0.0 0.001275958391978344 1.9630129107359138E-4 28-29 1.9630129107359138E-4 0.0 0.0 0.0027482180750302795 1.9630129107359138E-4 30-31 1.9630129107359138E-4 0.0 0.0 0.005594586795597354 2.9445193661038706E-4 32-33 1.9630129107359138E-4 0.0 0.0 0.011091022945657914 3.9260258214718276E-4 34-35 1.9630129107359138E-4 0.0 0.0 0.01619485651357129 3.9260258214718276E-4 36-37 1.9630129107359138E-4 0.0 0.0 0.021004238144874277 3.9260258214718276E-4 38-39 1.9630129107359138E-4 0.0 0.0 0.027678482041376386 3.9260258214718276E-4 40-41 1.9630129107359138E-4 0.0 0.0 0.03337121948251054 3.9260258214718276E-4 42-43 1.9630129107359138E-4 0.0 0.0 0.047799364376419504 3.9260258214718276E-4 44-45 1.9630129107359138E-4 0.0 0.0 0.06752764412931545 3.9260258214718276E-4 46-47 1.9630129107359138E-4 0.0 0.0 0.08480215774379149 3.9260258214718276E-4 48-49 1.9630129107359138E-4 0.0 0.0 0.1092416684824536 3.9260258214718276E-4 50-51 1.9630129107359138E-4 0.0 0.0 0.14222028538281697 3.9260258214718276E-4 52-53 1.9630129107359138E-4 0.0 0.0 0.19963841302184243 3.9260258214718276E-4 54-55 1.9630129107359138E-4 0.0 0.0 0.23909497252763431 3.9260258214718276E-4 56-57 1.9630129107359138E-4 0.0 0.0 0.3172228863749237 3.9260258214718276E-4 58-59 1.9630129107359138E-4 0.0 0.0 0.40281024928300957 3.9260258214718276E-4 60-61 1.9630129107359138E-4 0.0 0.0 0.4950718560875975 3.9260258214718276E-4 62-63 1.9630129107359138E-4 0.0 0.0 0.6373902921159512 3.9260258214718276E-4 64-65 1.9630129107359138E-4 0.0 0.0 0.7115921801417688 3.9260258214718276E-4 66-67 1.9630129107359138E-4 0.0 0.0 0.8722647868855034 3.9260258214718276E-4 68-69 1.9630129107359138E-4 0.0 0.0 1.0800497034868999 3.9260258214718276E-4 70-71 2.9445193661038706E-4 0.0 0.0 1.241801967331539 4.907532276839785E-4 72-73 3.9260258214718276E-4 0.0 0.0 1.5189793903274502 5.889038732207742E-4 74-75 3.9260258214718276E-4 0.0 0.0 1.7905622265277639 5.889038732207742E-4 76-77 3.9260258214718276E-4 0.0 0.0 1.9688037988225848 5.889038732207742E-4 78-79 3.9260258214718276E-4 0.0 0.0 2.1732515934757304 5.889038732207742E-4 80-81 3.9260258214718276E-4 0.0 0.0 2.52796802644571 5.889038732207742E-4 82-83 3.9260258214718276E-4 0.0 0.0 2.8264441395231055 5.889038732207742E-4 84-85 3.9260258214718276E-4 0.0 0.0 3.2770537531825346 5.889038732207742E-4 86-87 4.907532276839785E-4 0.0 0.0 3.833666064021703 5.889038732207742E-4 88-89 5.889038732207742E-4 0.0 0.0 4.586383364643389 5.889038732207742E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1680 0.0 56.014557 1 GTATCAA 3050 0.0 42.696835 1 TCCGTGC 45 9.6205075E-4 42.221786 8 ATCAACG 4055 0.0 32.450443 3 TATCAAC 4065 0.0 32.253754 2 TCAACGC 4075 0.0 32.058033 4 GTAAGGT 135 1.19343895E-8 31.669449 4 CAACGCA 4155 0.0 31.555119 5 AACGCAG 4240 0.0 30.807465 6 TGGTATC 725 0.0 30.140577 2 GTGGTAT 770 0.0 29.627533 1 AGGACCG 120 5.3702934E-6 27.710766 5 ACGCAGA 4775 0.0 27.256264 7 CGCAGAG 4765 0.0 27.21378 8 ATGGGCG 70 0.008383668 27.14524 5 ACGCTCT 70 0.008387684 27.142578 9 ATGCGCC 45 8.947384E-4 26.388617 82-83 CTATGCA 130 9.999971E-6 25.579168 4 AGGGACG 95 0.0012249897 25.002195 5 GTACATG 2320 0.0 24.99291 1 >>END_MODULE