FastQCFastQC Report
Thu 26 May 2016
SRR937208_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937208_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574659
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31370.5458889532748987No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25220.43886896402910247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17260.30035203485893375No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7830.13625471801537956No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6090.10597589178973967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14050.051.4144251
GTATCAA24050.039.7190361
TACACGA550.002575337234.554245
ATCAACG32900.029.1690083
TCAACGC33250.028.721594
TATCAAC33550.028.4597992
CAACGCA33900.028.1708815
AACGCAG34450.027.9969586
TAAGGTG1554.5676643E-827.587665
CGCCCGA350.00838472427.14739230-31
TACCTGG4250.025.7079352
GTACCTG5300.025.1072921
GTACATG28200.024.0993121
TACATGG27450.023.8816912
GGGTAGG1601.8485298E-623.7622591
CTACACG1201.7064142E-423.756044
ACGCAGA40600.023.4049647
CGCAGAG40650.023.3761798
ACTATAC1050.002192758722.6228263
CTTACAC2755.0931703E-1122.4582983
Produced by FastQC (version 0.11.5)