##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937208_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 574659 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77316112685958 33.0 31.0 34.0 30.0 34.0 2 31.855027068226548 33.0 31.0 34.0 30.0 34.0 3 31.891909810861748 33.0 31.0 34.0 30.0 34.0 4 35.507025905798045 37.0 35.0 37.0 33.0 37.0 5 35.52596235332606 37.0 35.0 37.0 33.0 37.0 6 35.56274764686536 37.0 35.0 37.0 33.0 37.0 7 35.450385358969406 37.0 35.0 37.0 33.0 37.0 8 35.45555886186417 37.0 35.0 37.0 33.0 37.0 9 37.07877367273461 39.0 37.0 39.0 33.0 39.0 10-11 36.976285936529315 39.0 37.0 39.0 33.0 39.0 12-13 36.81676002638086 39.0 37.0 39.0 33.0 39.0 14-15 38.001730591533416 40.0 38.0 41.0 33.0 41.0 16-17 37.93380944177329 40.0 38.0 41.0 33.0 41.0 18-19 37.91286571688602 40.0 38.0 41.0 33.0 41.0 20-21 37.81083042291168 40.0 38.0 41.0 33.0 41.0 22-23 37.66336818878674 40.0 37.5 41.0 32.5 41.0 24-25 37.60372064128466 40.0 37.0 41.0 32.0 41.0 26-27 37.60345787675821 40.0 37.0 41.0 32.0 41.0 28-29 37.523667949166374 40.0 37.0 41.0 32.0 41.0 30-31 37.28741740754082 39.5 37.0 41.0 32.0 41.0 32-33 37.03997240102392 39.0 36.0 41.0 31.0 41.0 34-35 37.06384829960028 39.5 36.0 41.0 31.0 41.0 36-37 36.94065263051653 39.5 36.0 41.0 31.0 41.0 38-39 36.817127200652905 40.0 36.0 41.0 31.0 41.0 40-41 36.59919969929993 39.0 35.5 41.0 30.0 41.0 42-43 36.322089273812814 39.0 35.0 41.0 30.0 41.0 44-45 36.06757920784326 39.0 35.0 41.0 29.5 41.0 46-47 35.697797302400204 39.0 35.0 41.0 27.5 41.0 48-49 35.339914627631345 39.0 35.0 40.0 26.5 41.0 50-51 34.33226487360331 38.0 34.0 39.5 25.0 40.5 52-53 34.432034476097996 38.0 34.0 39.5 24.5 40.5 54-55 34.785416220750044 38.0 34.5 40.0 25.0 41.0 56-57 34.56857458075137 38.0 34.0 40.0 24.0 41.0 58-59 34.295196803669654 38.0 34.0 40.0 23.0 41.0 60-61 33.98272105718348 38.0 34.0 40.0 22.5 41.0 62-63 33.65624222364916 37.0 33.5 40.0 22.0 41.0 64-65 33.21842518780703 36.5 33.0 39.5 20.0 41.0 66-67 32.86427690160599 36.0 32.5 39.0 19.5 41.0 68-69 32.38697384013824 35.5 32.0 39.0 17.5 40.0 70-71 31.917496289103624 35.0 31.5 38.5 13.5 40.0 72-73 31.46328953344505 35.0 31.0 37.5 9.5 40.0 74-75 30.9773317741478 35.0 31.0 37.0 7.0 39.0 76-77 30.499082934401095 35.0 30.0 36.5 4.0 39.0 78-79 30.005526755867393 34.0 30.0 36.0 2.0 38.5 80-81 29.53993063712567 34.0 29.0 35.5 2.0 37.0 82-83 29.075094099283227 34.0 29.0 35.0 2.0 37.0 84-85 28.570493979908086 34.0 28.5 35.0 2.0 36.5 86-87 28.117132073107705 34.0 27.5 35.0 2.0 36.0 88-89 27.65587504937711 33.0 26.5 35.0 2.0 35.5 90-91 27.161510565396174 33.0 26.0 35.0 2.0 35.0 92-93 26.666715391214616 33.0 25.0 35.0 2.0 35.0 94-95 26.116125389143825 32.5 24.5 35.0 2.0 35.0 96-97 25.396794446793663 32.0 21.5 34.5 2.0 35.0 98-99 24.503703065644146 32.0 10.0 34.0 2.0 35.0 100-101 21.95381521911255 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.023498993836977178 1101 2 0.1329041018349848 1101 3 0.044512807185864034 1101 4 0.2676107846024678 1101 5 0.08132949571565717 1101 6 0.13454027624645448 1101 7 0.06666986758555282 1101 8 -0.024541854359306114 1101 9 0.039270703520884354 1101 10-11 0.018790037739350396 1101 12-13 0.1918924398396129 1101 14-15 -0.07797224408766823 1101 16-17 -0.061075584007163286 1101 18-19 -0.0237158170895313 1101 20-21 -0.09549460192636872 1101 22-23 -0.10055262339162141 1101 24-25 -0.05695821835468706 1101 26-27 -0.05913248898937695 1101 28-29 0.01350405937306931 1101 30-31 0.19885747726119263 1101 32-33 -0.08028070550370359 1101 34-35 -2.2155556072078753E-4 1101 36-37 0.06806914969076416 1101 38-39 0.06000702636146826 1101 40-41 -0.0780876337562404 1101 42-43 0.1628342691626159 1101 44-45 0.25353786603627526 1101 46-47 0.5032871025660839 1101 48-49 0.3595524172961788 1101 50-51 0.5621930750245383 1101 52-53 0.7560921943793844 1101 54-55 0.614560707729396 1101 56-57 0.746245012315292 1101 58-59 0.6117746752306914 1101 60-61 0.8043486579665142 1101 62-63 0.5253010564015383 1101 64-65 0.43424779183518325 1101 66-67 0.4646590197184537 1101 68-69 0.3773449479449056 1101 70-71 0.4619192905572511 1101 72-73 0.3366943735365808 1101 74-75 0.37752249121799153 1101 76-77 0.4511717401123789 1101 78-79 0.0104542138074315 1101 80-81 0.2691688594181656 1101 82-83 0.42669777671964226 1101 84-85 0.3711095807712894 1101 86-87 0.08398704419032654 1101 88-89 -0.43324319662044886 1101 90-91 -0.22977238478872408 1101 92-93 0.01397700355472864 1101 94-95 -0.09591012748155947 1101 96-97 0.09264346044993843 1101 98-99 0.17270178044745066 1101 100-101 0.17148799055649633 1102 1 -0.18271204618926973 1102 2 -0.16904594737761158 1102 3 -0.1527066810031137 1102 4 -0.2563701045051445 1102 5 0.007572113825894178 1102 6 -0.13009021916036545 1102 7 -0.05633087060341779 1102 8 -0.19947213651153817 1102 9 -0.1317123181326636 1102 10-11 -0.21710747702442745 1102 12-13 -0.17463376586904644 1102 14-15 0.02139619423254402 1102 16-17 -0.01402116307540524 1102 18-19 -0.1739577790317881 1102 20-21 -0.04885541229381829 1102 22-23 -0.31499139471708304 1102 24-25 -0.22173327576937396 1102 26-27 -0.14983233314948308 1102 28-29 -0.06672785499700495 1102 30-31 0.01807807273363693 1102 32-33 0.05963686339393348 1102 34-35 0.13896295067287667 1102 36-37 6.581424729077412E-4 1102 38-39 -0.028160419504665413 1102 40-41 0.02521781243798671 1102 42-43 -0.06904934304210997 1102 44-45 0.08054532994703578 1102 46-47 0.09385366260544714 1102 48-49 0.15687853278175368 1102 50-51 0.3047599221374 1102 52-53 0.10321018158411022 1102 54-55 0.37323955451417135 1102 56-57 0.23001510745964993 1102 58-59 0.2596841650600865 1102 60-61 0.23730161892451207 1102 62-63 0.16813549701834063 1102 64-65 0.005313859748575567 1102 66-67 -0.017665455347163572 1102 68-69 -0.11619077219944529 1102 70-71 -0.19143434297293282 1102 72-73 -0.1114581034975366 1102 74-75 -0.13821016134106756 1102 76-77 -0.07767914571439505 1102 78-79 -0.2105616162450623 1102 80-81 -0.24629722489941486 1102 82-83 -0.49340864552445396 1102 84-85 -0.2036628109609957 1102 86-87 -0.26953883343434626 1102 88-89 -0.4215193203081107 1102 90-91 -0.28019991923754617 1102 92-93 -0.32127869690458866 1102 94-95 -0.1493416809592496 1102 96-97 -0.48117031087551965 1102 98-99 -0.1747853012454641 1102 100-101 0.3176699131286753 1103 1 0.14263489429658094 1103 2 -0.2606104270716436 1103 3 -0.0639097254701575 1103 4 -0.0629653069553413 1103 5 -0.03188939035110394 1103 6 -0.04209895028958499 1103 7 0.09874571724689929 1103 8 -0.0033645744850971937 1103 9 0.07898141046331375 1103 10-11 -0.13470744502552634 1103 12-13 -0.06031958997412801 1103 14-15 -0.19437736019796858 1103 16-17 -0.06250711974736589 1103 18-19 -0.19334949512532518 1103 20-21 -0.1684055991445561 1103 22-23 -0.19653355136694728 1103 24-25 -0.004981659634317737 1103 26-27 -0.24114729758879605 1103 28-29 -0.23862481852969353 1103 30-31 -0.27176627546014487 1103 32-33 -0.3646885883048654 1103 34-35 0.04310909423367093 1103 36-37 -0.02373244984963918 1103 38-39 -0.303564310418885 1103 40-41 -0.0673761557688124 1103 42-43 -0.015789264340050124 1103 44-45 0.13179023964536185 1103 46-47 -0.037611353832083694 1103 48-49 0.0569248169091523 1103 50-51 0.1837554994318964 1103 52-53 0.38558145266434707 1103 54-55 0.11720928507578776 1103 56-57 0.08731714281843495 1103 58-59 -0.035454287335831225 1103 60-61 0.07803088915905221 1103 62-63 -0.2569080252989906 1103 64-65 0.006946184735532768 1103 66-67 0.2069258919971162 1103 68-69 -0.04969019507637995 1103 70-71 0.12917085532503236 1103 72-73 0.23125586059512315 1103 74-75 -0.20086991776846475 1103 76-77 -0.18508022529642787 1103 78-79 -0.057569718824399985 1103 80-81 0.008035007519282544 1103 82-83 0.023077455904449096 1103 84-85 -0.10751848829015032 1103 86-87 0.010922026955203279 1103 88-89 -0.03719725896926107 1103 90-91 -0.019476477374599455 1103 92-93 0.10052207944974256 1103 94-95 0.28907471619284664 1103 96-97 0.3848685677914965 1103 98-99 0.40709284388479716 1103 100-101 0.681976470237192 1104 1 0.18583048893501442 1104 2 -0.04247886385129007 1104 3 -0.01449454575531206 1104 4 -0.04220845559360953 1104 5 0.03381111336271658 1104 6 0.032809271344497404 1104 7 -0.03478540202228686 1104 8 -0.017709746516167968 1104 9 -0.11157182098892093 1104 10-11 -0.06266676373123659 1104 12-13 -0.14923041555254457 1104 14-15 -0.007567832322962431 1104 16-17 -0.15806547371304447 1104 18-19 -0.12681017983462795 1104 20-21 -0.1027403127106794 1104 22-23 -0.36045611603867656 1104 24-25 -0.21802592668802134 1104 26-27 -0.1427032610481973 1104 28-29 -0.13821928621516832 1104 30-31 -0.1767169712682204 1104 32-33 -0.19540019079781956 1104 34-35 -0.2256734550705204 1104 36-37 -0.46141920815237825 1104 38-39 -0.2606195055012748 1104 40-41 -0.35278126120721254 1104 42-43 -0.09873129456288154 1104 44-45 -0.09479240357156726 1104 46-47 -0.07195033615941071 1104 48-49 -0.3947367983900918 1104 50-51 -0.41021807693624623 1104 52-53 -0.22519610218923702 1104 54-55 -0.061917539819631884 1104 56-57 -0.3810374631749056 1104 58-59 -0.24356386643597006 1104 60-61 -0.17773620722957162 1104 62-63 -0.010996431343549773 1104 64-65 -0.21565263708637872 1104 66-67 -0.15758362734037235 1104 68-69 -0.2661676133295998 1104 70-71 -0.3307967020898097 1104 72-73 -0.20799006273792742 1104 74-75 0.051059562296419614 1104 76-77 -0.12104026969154447 1104 78-79 -0.22886870285923422 1104 80-81 -0.19457960871908853 1104 82-83 -0.35753169631957604 1104 84-85 -0.43549274766008494 1104 86-87 -0.022148066103792274 1104 88-89 -0.18688137064005517 1104 90-91 0.1345735098191163 1104 92-93 0.1921862560057086 1104 94-95 -0.06549499267763892 1104 96-97 0.08635516388131492 1104 98-99 0.16949252799646786 1104 100-101 0.3068740199682587 1105 1 -0.13901087618246066 1105 2 -0.10058639257706403 1105 3 -0.1797492190532033 1105 4 -0.07991639591259059 1105 5 -0.14915064276830492 1105 6 -0.23066183138230656 1105 7 -0.22525873421230358 1105 8 -0.2680656031445352 1105 9 -0.026740745701665958 1105 10-11 -0.40852296335394556 1105 12-13 -0.18955154400392615 1105 14-15 -0.18415846857745777 1105 16-17 -0.3049656623599333 1105 18-19 -0.17330735013131715 1105 20-21 -0.13261726349099234 1105 22-23 -0.20089086136538015 1105 24-25 -0.024111932518913193 1105 26-27 -0.19720077433922967 1105 28-29 -0.5550603083382981 1105 30-31 -0.14682287077088318 1105 32-33 -0.42780029369612294 1105 34-35 -0.39725637395235225 1105 36-37 -0.1996930891333406 1105 38-39 0.08283144630802752 1105 40-41 -0.17577045688548765 1105 42-43 -0.21204804983641168 1105 44-45 0.019508316716546403 1105 46-47 -0.3927379369139956 1105 48-49 -0.4326912658913784 1105 50-51 -0.26080331102988197 1105 52-53 -0.2639951932542388 1105 54-55 0.010889440115207094 1105 56-57 0.0389784003133542 1105 58-59 0.17688073997802434 1105 60-61 0.12690279122695358 1105 62-63 0.23080814763088853 1105 64-65 0.06459088889543807 1105 66-67 -0.0026788082698914195 1105 68-69 0.09088092036959239 1105 70-71 -0.15206235120659883 1105 72-73 -0.08078846781424076 1105 74-75 0.06410192209748189 1105 76-77 0.21703210211432022 1105 78-79 0.20239951871511153 1105 80-81 0.0679096727077706 1105 82-83 -0.11653101033089541 1105 84-85 -0.04217470615776975 1105 86-87 0.04785707881131884 1105 88-89 0.24400526973037628 1105 90-91 0.42792585075549283 1105 92-93 0.4157949852116758 1105 94-95 0.24359006991882737 1105 96-97 0.14872225357433422 1105 98-99 0.21585464427154832 1105 100-101 0.11780267714541637 1106 1 -0.2805218893368675 1106 2 -0.23714299744731093 1106 3 -0.2626791784600684 1106 4 -0.06400928118700477 1106 5 -0.19901091497014534 1106 6 9.86398654752918E-4 1106 7 -0.1982012946951457 1106 8 -0.3857484931152868 1106 9 -0.19405141569762208 1106 10-11 -0.702448872747091 1106 12-13 -0.3848775212847926 1106 14-15 -0.39553105588990434 1106 16-17 -0.3683267552591545 1106 18-19 -0.5410727748247766 1106 20-21 -0.5776828495340212 1106 22-23 -0.4266372774984788 1106 24-25 -0.6289310303562772 1106 26-27 -0.41066695370228956 1106 28-29 6.626159354539141E-6 1106 30-31 -0.37485352253944626 1106 32-33 -0.23218835103799762 1106 34-35 -0.1459520928414193 1106 36-37 -0.5872401106760634 1106 38-39 -0.5557257537421947 1106 40-41 -0.37371662099706526 1106 42-43 -0.5103552742983126 1106 44-45 -0.24012615908334567 1106 46-47 -0.3997824498661373 1106 48-49 -0.2385835476160949 1106 50-51 -0.48741692298184347 1106 52-53 -0.34344241985505874 1106 54-55 -0.7240962151174983 1106 56-57 -0.6391280439048046 1106 58-59 -0.5933844405986477 1106 60-61 -0.5447124714194587 1106 62-63 -0.6824559960306757 1106 64-65 -0.5221635668171203 1106 66-67 -0.6585218016531584 1106 68-69 -0.840349056241692 1106 70-71 -0.775087911396497 1106 72-73 -0.9010510909769742 1106 74-75 -0.7966794376097646 1106 76-77 -0.299837530222554 1106 78-79 -0.29972197239671416 1106 80-81 -0.2702269489949103 1106 82-83 -0.5539173797795627 1106 84-85 -0.5064011349384394 1106 86-87 -0.4468548866310442 1106 88-89 0.056692135317351955 1106 90-91 0.15538573423041413 1106 92-93 0.3510203398784917 1106 94-95 0.4482703699267354 1106 96-97 0.3661152258008187 1106 98-99 0.8091432926085318 1106 100-101 1.159414126920133 1107 1 0.07453770601957999 1107 2 0.05970675918236523 1107 3 -0.22465757368933836 1107 4 0.051973544597068155 1107 5 0.04495135552117091 1107 6 0.08351000544688958 1107 7 -0.007266096082730655 1107 8 -0.11039494052894838 1107 9 -0.08894335307014956 1107 10-11 0.09608883737467977 1107 12-13 0.2205963076813191 1107 14-15 0.21930794229806594 1107 16-17 -0.17731659393422916 1107 18-19 -0.18285699077942041 1107 20-21 -0.05135672037584982 1107 22-23 -0.09319279255693402 1107 24-25 0.022634527120736436 1107 26-27 -0.009323053211417687 1107 28-29 0.06154713575070048 1107 30-31 -0.007474193119684003 1107 32-33 -0.10872605598992635 1107 34-35 0.19517219275100928 1107 36-37 0.09698721762701012 1107 38-39 0.2014919922582905 1107 40-41 0.22743134247282626 1107 42-43 0.10381422931928341 1107 44-45 0.0700243091751176 1107 46-47 -0.12805554161274557 1107 48-49 0.1630201374474538 1107 50-51 0.08697064458153392 1107 52-53 0.1949333191092606 1107 54-55 0.34986742538201554 1107 56-57 0.0940447658269079 1107 58-59 -0.2098726184619082 1107 60-61 0.1552221300761829 1107 62-63 0.2524259635463295 1107 64-65 0.27933258552778994 1107 66-67 0.14139625634730635 1107 68-69 0.18914747867154347 1107 70-71 0.49995932803699716 1107 72-73 0.390092452277802 1107 74-75 0.5414256601131804 1107 76-77 0.18506136058779532 1107 78-79 0.2181734365248822 1107 80-81 0.19567923387791808 1107 82-83 0.37394651895356645 1107 84-85 0.3994831795017042 1107 86-87 0.44625216270734214 1107 88-89 0.3059444468830641 1107 90-91 0.6118556625505498 1107 92-93 0.47516246918141647 1107 94-95 0.722852323558417 1107 96-97 0.37528523347803144 1107 98-99 0.6841662337834826 1107 100-101 0.8018187239438532 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1.4011388307150128 2315 68-69 1.4730480729449056 2315 70-71 1.5838788588562203 2315 72-73 1.7434678915778257 2315 74-75 1.779349434946507 2315 76-77 1.6831036559199362 2315 78-79 2.02018627995864 2315 80-81 1.8751141450710875 2315 82-83 1.7699041233691233 2315 84-85 1.8594713720084037 2315 86-87 1.9774779698776115 2315 88-89 1.783150972193983 2315 90-91 1.7209162427885936 2315 92-93 1.7675678003760318 2315 94-95 2.1795878858345894 2315 96-97 2.3046212847626535 2315 98-99 2.5681659664440097 2315 100-101 2.4886862293881116 2316 1 -0.262382467133488 2316 2 -0.09011475365024779 2316 3 -0.08266282572552797 2316 4 -0.1910357080135654 2316 5 0.18585876786755762 2316 6 0.0770013785671324 2316 7 0.3090716344631943 2316 8 -0.07949306743947915 2316 9 -0.3608644494327038 2316 10-11 -0.6092509037163438 2316 12-13 -0.2967266925443539 2316 14-15 -0.2399580035813429 2316 16-17 -0.23371700014640595 2316 18-19 0.06492474198220322 2316 20-21 -0.2570683097322757 2316 22-23 -0.21598478320174763 2316 24-25 0.09099306065375146 2316 26-27 -0.2976362469007654 2316 28-29 -0.023308638411741356 2316 30-31 0.11828489023587707 2316 32-33 0.22930196854692753 2316 34-35 0.11079341015658173 2316 36-37 0.01903499884687676 2316 38-39 -0.0032758718452754465 2316 40-41 0.4155173240496737 2316 42-43 0.40266483350860227 2316 44-45 0.3549190632936572 2316 46-47 0.3584014881146018 2316 48-49 0.5100007295324716 2316 50-51 0.6364777532945851 2316 52-53 0.7707365113625073 2316 54-55 0.651587672391841 2316 56-57 0.7383501019777441 2316 58-59 0.9702019563488378 2316 60-61 1.042423882122634 2316 62-63 0.9043144398403626 2316 64-65 1.0217263651474227 2316 66-67 0.8200783235159221 2316 68-69 1.139714739611577 2316 70-71 1.1614594408737027 2316 72-73 1.0141897585576807 2316 74-75 1.0881221088340247 2316 76-77 1.2898122991841063 2316 78-79 1.1068907908116508 2316 80-81 1.2966527069920062 2316 82-83 0.9405509544622532 2316 84-85 0.9742708413198109 2316 86-87 0.7386300426080474 2316 88-89 0.5825713471559233 2316 90-91 0.5310645745994584 2316 92-93 0.5946155188500875 2316 94-95 0.6698549700922776 2316 96-97 0.7212118280659752 2316 98-99 0.8752911132533576 2316 100-101 0.7603922627083968 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2464.0 3 603.0 4 462.0 5 550.0 6 511.0 7 632.0 8 773.0 9 943.0 10 1209.0 11 1544.0 12 2125.0 13 2712.0 14 3180.0 15 3952.0 16 4867.0 17 6602.0 18 5688.0 19 4001.0 20 3816.0 21 4057.0 22 4288.0 23 4844.0 24 5405.0 25 6105.0 26 6948.0 27 8155.0 28 9760.0 29 11673.0 30 14151.0 31 17426.0 32 22312.0 33 29190.0 34 39847.0 35 57521.0 36 82570.0 37 111619.0 38 82708.0 39 9446.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.04946281568299 18.95946700153481 14.612808706571787 22.378261476210408 2 15.399195453808343 20.14818018068287 40.491655492414004 23.96096887309478 3 19.27505605596181 24.724776560761875 29.677229248832678 26.32293813444364 4 12.397626557724061 16.347493430837662 36.88007072202374 34.374809289414536 5 13.321011656190334 37.90171137633715 33.93825793571223 14.839019031760287 6 31.846047323635133 36.248606922917276 17.228461153424224 14.67688460002337 7 28.53764247281353 33.32839165961751 20.056858316400834 18.077107551168126 8 26.871692025984217 35.07555478048568 19.204429524349305 18.8483236691808 9 26.57660092809448 16.52015143879058 19.833877715443652 37.06936991767129 10-11 25.089122207300697 27.133264733069396 26.694455587142684 21.083157472487223 12-13 26.153098882393156 24.340388720682004 27.638881137419595 21.867631259505238 14-15 22.673459494147682 25.67390591649112 26.062258478536847 25.590376110824348 16-17 21.790842514480254 29.28796041627788 26.72119253462442 22.20000453461744 18-19 21.845356482979135 27.1383441876363 29.73657570211456 21.27972362727001 20-21 22.484868142273466 26.17519299548878 30.106424347686584 21.23351451455117 22-23 22.0876327984341 26.28739826234039 30.0018745449869 21.623094394238606 24-25 21.87450958510464 26.525735230011126 30.11749033119788 21.48226485368635 26-27 21.42695216521668 26.97230636163731 29.89364429168563 21.70709718146038 28-29 21.47709060342407 27.032181105699056 29.966551042794787 21.52417724808209 30-31 21.539686332094544 26.846718485409195 29.857919914057856 21.75567526843841 32-33 21.349034722101116 26.93221255297257 29.786191381385045 21.93256134354127 34-35 21.83586691874621 27.110788260162828 29.632920797259683 21.420424023831284 36-37 21.83283897529256 26.775809064451384 29.58353604628656 21.807815913969495 38-39 21.513437070323704 27.174592545898257 29.694255753793357 21.61771462998468 40-41 21.78628380811386 27.046665463476643 29.626374622804285 21.540676105605208 42-43 21.576758420451476 27.11197914396073 29.35740380675031 21.953858628837487 44-45 21.61300280440333 27.396474265064946 29.31595579925495 21.67456713127677 46-47 21.940725160151597 27.70199681528107 28.58581736177605 21.771460662791288 48-49 21.93328909078066 27.868447879976525 28.32401245651687 21.87425057272595 50-51 21.982251926370612 28.07207713156527 28.156404898859 21.789266043205117 52-53 22.23152562418559 27.938988289924243 28.107147280412093 21.722338805478074 54-55 21.85331821749368 28.18941309845644 28.144937647161424 21.812331036888462 56-57 21.918145032264363 28.389589260382706 27.95547421771667 21.736791489636257 58-59 21.89060451362736 27.96468323709097 28.127963985828664 22.01674826345301 60-61 21.639914185164425 28.009163810966758 28.43754059074849 21.913381413120327 62-63 21.762692004906537 28.07292851781235 28.51040198214362 21.65397749513749 64-65 21.55935790837275 28.083488649023735 28.38214808137903 21.975005361224486 66-67 21.442002498415608 28.38473856861533 28.137948108622673 22.03531082434639 68-69 21.7051087356398 28.045478400200857 28.428358966222472 21.821053897936874 70-71 21.56470781054937 28.238616153937357 28.039673461986425 22.15700257352685 72-73 21.653916401853472 28.234447946226577 27.944308586921935 22.167327064998016 74-75 21.581706446317305 28.466984648138187 27.82785409584953 22.123454809694977 76-77 21.702146952554997 28.391145738878116 27.89259657101405 22.014110737552837 78-79 21.744437363064666 28.372347279776594 27.679486095260348 22.20372926189839 80-81 21.743853436243214 28.50866983486818 27.71077824943353 22.036698479455076 82-83 21.729298072019215 28.536382300761513 27.60756894830675 22.12675067891252 84-85 21.538625170766068 28.553307347539793 27.57356563040251 22.334501851291634 86-87 21.825156717263745 28.429775844180753 27.55041544242657 22.19465199612893 88-89 21.699966260131767 28.35360776836491 27.515516416145964 22.43090955535736 90-91 21.435832961330284 28.32492257686513 27.86439442553429 22.374850036270296 92-93 21.627884674065616 28.022423518539508 27.934106938910734 22.415584868484146 94-95 21.37963141362352 28.008135787088577 27.974919596767656 22.637313202520247 96-97 21.453152341750798 27.861938844282314 27.96569309144934 22.71921572251755 98-99 21.39532085526528 28.160227531325226 27.686174873726916 22.758276739682575 100-101 21.529432891821067 27.82285693363717 27.99581211348789 22.651898061053878 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 972.0 1 591.5 2 233.5 3 249.5 4 236.0 5 262.0 6 373.5 7 704.5 8 1124.0 9 1306.5 10 1172.0 11 991.0 12 974.5 13 1013.0 14 1091.0 15 1117.5 16 1122.5 17 1164.0 18 1242.0 19 1353.5 20 1514.5 21 1702.0 22 1929.0 23 2210.5 24 2570.5 25 3114.0 26 3893.0 27 4825.0 28 5600.5 29 6438.5 30 7342.0 31 8322.0 32 9860.5 33 10940.0 34 11530.5 35 12209.5 36 13134.5 37 14393.5 38 15451.0 39 16050.0 40 16570.0 41 17579.0 42 18159.0 43 18591.0 44 19402.5 45 20193.0 46 20552.5 47 20958.5 48 21732.5 49 21470.5 50 20749.0 51 20227.5 52 19637.0 53 19255.0 54 18820.0 55 17458.5 56 15735.0 57 14249.5 58 12915.5 59 11401.5 60 9338.5 61 7542.5 62 6047.5 63 4467.0 64 3144.0 65 2296.0 66 1656.0 67 1266.0 68 955.0 69 666.0 70 507.5 71 354.0 72 243.0 73 200.0 74 134.5 75 92.5 76 78.5 77 52.0 78 35.5 79 27.5 80 16.0 81 9.0 82 7.5 83 6.0 84 4.5 85 2.5 86 2.0 87 3.0 88 4.5 89 2.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22604709923624272 2 0.20325097144567472 3 0.19576827301060282 4 0.1994226141068007 5 0.19907458162144853 6 0.2241329205668057 7 0.22691718044962317 8 0.2147360434622968 9 0.21334391352088805 10-11 0.2243069368094818 12-13 0.22465496929483397 14-15 0.21073366988074668 16-17 0.22482898553751007 18-19 0.21412698661293045 20-21 0.20881949121130966 22-23 0.20647027193518244 24-25 0.20272892271764645 26-27 0.2098635886673662 28-29 0.21717227085976204 30-31 0.2175203033451142 32-33 0.2173462871024381 34-35 0.22535103426553835 36-37 0.20673129629919657 38-39 0.20699232066321072 40-41 0.20594822320715417 42-43 0.20951555618201403 44-45 0.2218707094120165 46-47 0.22552505050821445 48-49 0.2270041885709612 50-51 0.2263951317215949 52-53 0.2422306098051192 54-55 0.22778726166300364 56-57 0.20768838563391506 58-59 0.19263598064243317 60-61 0.1911568425796864 62-63 0.19811749228673003 64-65 0.19072180197299615 66-67 0.1906347938516581 68-69 0.19350606185581362 70-71 0.19611630549595502 72-73 0.1986395410147583 74-75 0.19933560598546268 76-77 0.2079494099979292 78-79 0.2031639633243367 80-81 0.19968363847081486 82-83 0.1968993785879974 84-85 0.19846552477208224 86-87 0.20429506890173127 88-89 0.20072773592687143 90-91 0.20655728005652046 92-93 0.20081474404820945 94-95 0.1989875735001105 96-97 0.20681830442053464 98-99 0.2087324830899716 100-101 0.19141786694370053 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 574659.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.32067967331021 #Duplication Level Percentage of deduplicated Percentage of total 1 85.69856713520299 61.120800551114826 2 8.936782788649108 12.747548451583897 3 2.3532059036060655 5.034967333692921 4 0.985200189347409 2.8106058847412454 5 0.5369941631761482 1.9149394349161668 6 0.34133735423931805 1.460664726134257 7 0.22370107236731554 1.116815876741971 8 0.16760912552400747 0.9563197401457104 9 0.12258199413411142 0.7868368023619097 >10 0.5895368253902175 7.1931295137736955 >50 0.028352101899419245 1.393673645438025 >100 0.014909274762077582 1.9337092275090162 >500 4.888286807220893E-4 0.24265061252023248 >1k 7.33243021083134E-4 1.2873381993260897 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3137 0.5458889532748987 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2522 0.43886896402910247 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1726 0.30035203485893375 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 783 0.13625471801537956 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 609 0.10597589178973967 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.740162426760914E-4 2 0.0 0.0 0.0 0.0 1.740162426760914E-4 3 0.0 0.0 0.0 0.0 1.740162426760914E-4 4 0.0 0.0 0.0 0.0 1.740162426760914E-4 5 0.0 0.0 0.0 0.0 1.740162426760914E-4 6 0.0 0.0 0.0 0.0 1.740162426760914E-4 7 0.0 0.0 0.0 0.0 1.740162426760914E-4 8 0.0 0.0 0.0 0.0 1.740162426760914E-4 9 0.0 0.0 0.0 1.740162426760914E-4 1.740162426760914E-4 10-11 0.0 0.0 0.0 1.740162426760914E-4 1.740162426760914E-4 12-13 0.0 0.0 0.0 1.740162426760914E-4 1.740162426760914E-4 14-15 0.0 0.0 0.0 1.740162426760914E-4 1.740162426760914E-4 16-17 0.0 0.0 0.0 3.480324853521828E-4 1.740162426760914E-4 18-19 0.0 0.0 0.0 5.220487280282742E-4 1.740162426760914E-4 20-21 0.0 0.0 0.0 6.090568493663199E-4 1.740162426760914E-4 22-23 0.0 0.0 0.0 7.830730920424113E-4 1.740162426760914E-4 24-25 0.0 0.0 0.0 0.0010440974560565483 1.740162426760914E-4 26-27 0.0 0.0 0.0 0.0013921299414087311 1.740162426760914E-4 28-29 1.740162426760914E-4 0.0 0.0 0.0024362273974652794 1.740162426760914E-4 30-31 1.740162426760914E-4 0.0 0.0 0.006090568493663198 1.740162426760914E-4 32-33 1.740162426760914E-4 0.0 0.0 0.01174609638063617 1.740162426760914E-4 34-35 1.740162426760914E-4 0.0 0.0 0.016270518690214543 1.740162426760914E-4 36-37 1.740162426760914E-4 0.0 0.0 0.021142973485145104 1.740162426760914E-4 38-39 1.740162426760914E-4 0.0 0.0 0.02845165567754094 1.740162426760914E-4 40-41 1.740162426760914E-4 0.0 0.0 0.03402017544317587 1.740162426760914E-4 42-43 1.740162426760914E-4 0.0 0.0 0.049507621041347996 1.740162426760914E-4 44-45 1.740162426760914E-4 0.0 0.0 0.06943248082776046 1.740162426760914E-4 46-47 4.350406066902285E-4 0.0 0.0 0.08787820255142614 1.740162426760914E-4 48-49 5.220487280282742E-4 0.0 0.0 0.11128338719136044 1.740162426760914E-4 50-51 5.220487280282742E-4 0.0 0.0 0.1416492215383384 1.740162426760914E-4 52-53 5.220487280282742E-4 0.0 0.0 0.19942261410680073 1.740162426760914E-4 54-55 5.220487280282742E-4 0.0 0.0 0.2465810158720215 1.740162426760914E-4 56-57 5.220487280282742E-4 0.0 0.0 0.32906471490048883 1.740162426760914E-4 58-59 5.220487280282742E-4 0.0 0.0 0.4228594697029021 2.610243640141371E-4 60-61 5.220487280282742E-4 0.0 0.0 0.518046354446724 3.480324853521828E-4 62-63 5.220487280282742E-4 0.0 0.0 0.6497766501525253 3.480324853521828E-4 64-65 5.220487280282742E-4 0.0 0.0 0.7252996994739489 3.480324853521828E-4 66-67 5.220487280282742E-4 0.0 0.0 0.8853946427359529 3.480324853521828E-4 68-69 5.220487280282742E-4 0.0 0.0 1.0844692243574015 3.480324853521828E-4 70-71 5.220487280282742E-4 0.0 0.0 1.247522443744899 3.480324853521828E-4 72-73 5.220487280282742E-4 0.0 0.0 1.5108090189138255 3.480324853521828E-4 74-75 5.220487280282742E-4 0.0 0.0 1.7700932205012014 3.480324853521828E-4 76-77 5.220487280282742E-4 0.0 0.0 1.9500260154282802 3.480324853521828E-4 78-79 5.220487280282742E-4 0.0 0.0 2.1579754254262093 3.480324853521828E-4 80-81 5.220487280282742E-4 0.0 0.0 2.502005537196842 3.480324853521828E-4 82-83 5.220487280282742E-4 0.0 0.0 2.808883181156129 5.220487280282742E-4 84-85 5.220487280282742E-4 0.0 0.0 3.2307855615243124 5.220487280282742E-4 86-87 5.220487280282742E-4 0.0 0.0 3.755270516950052 5.220487280282742E-4 88-89 6.960649707043656E-4 0.0 0.0 4.4145310523284245 5.220487280282742E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1405 0.0 51.414425 1 GTATCAA 2405 0.0 39.719036 1 TACACGA 55 0.0025753372 34.55424 5 ATCAACG 3290 0.0 29.169008 3 TCAACGC 3325 0.0 28.72159 4 TATCAAC 3355 0.0 28.459799 2 CAACGCA 3390 0.0 28.170881 5 AACGCAG 3445 0.0 27.996958 6 TAAGGTG 155 4.5676643E-8 27.58766 5 CGCCCGA 35 0.008384724 27.147392 30-31 TACCTGG 425 0.0 25.707935 2 GTACCTG 530 0.0 25.107292 1 GTACATG 2820 0.0 24.099312 1 TACATGG 2745 0.0 23.881691 2 GGGTAGG 160 1.8485298E-6 23.762259 1 CTACACG 120 1.7064142E-4 23.75604 4 ACGCAGA 4060 0.0 23.404964 7 CGCAGAG 4065 0.0 23.376179 8 ACTATAC 105 0.0021927587 22.622826 3 CTTACAC 275 5.0931703E-11 22.458298 3 >>END_MODULE