FastQCFastQC Report
Thu 26 May 2016
SRR937208_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937208_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574659
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50870.8852206264932768No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44080.7670635977162108No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31140.5418865796933485No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11280.1962903217386311No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8810.15330830979763652No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCG200.002074052371.245635
GGTATCA18900.058.343911
GTATCAA32350.039.0819931
GTGGTAT8000.029.7063581
TATCAAC42450.029.5388242
ATCAACG42800.029.297273
TGGTATC7950.029.2749332
CGTACAC650.00584342529.2289753
TCAACGC43050.029.1271324
GTATAGG1652.6229827E-928.8061661
CAACGCA44200.028.261845
AACGCAG45050.027.8340326
CGCAGAG52350.024.1362448
ACGCAGA52500.024.0672847
GAGTACT39100.022.47500212-13
GTACATG25700.022.3780171
TACATGG26050.022.2443162
GCAGAGT56150.022.1644159
GTAAGGT2154.4146873E-822.0916674
GTATTAG1100.002865056521.6046241
Produced by FastQC (version 0.11.5)