##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937208_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 574659 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26968515241212 33.0 31.0 34.0 31.0 34.0 2 32.463535766428436 34.0 31.0 34.0 31.0 34.0 3 32.50948301514463 34.0 31.0 34.0 31.0 34.0 4 36.14499381372257 37.0 35.0 37.0 35.0 37.0 5 36.08082532423576 37.0 35.0 37.0 35.0 37.0 6 36.127764465535215 37.0 35.0 37.0 35.0 37.0 7 36.075018402217665 37.0 35.0 37.0 35.0 37.0 8 36.0506230651569 37.0 35.0 37.0 35.0 37.0 9 37.77278351161297 39.0 37.0 39.0 35.0 39.0 10-11 37.69830368966639 39.0 37.0 39.0 35.0 39.0 12-13 37.594469937824 39.0 37.0 39.0 35.0 39.0 14-15 38.792921541296664 40.0 38.0 41.0 35.0 41.0 16-17 38.76667815173869 40.0 38.0 41.0 35.0 41.0 18-19 38.78742784851538 40.0 38.0 41.0 35.0 41.0 20-21 38.70754047182764 40.0 38.0 41.0 34.5 41.0 22-23 38.563294927948576 40.0 38.0 41.0 34.0 41.0 24-25 38.50043504060669 40.0 38.0 41.0 34.0 41.0 26-27 38.43331436556288 40.0 38.0 41.0 34.0 41.0 28-29 38.29888246768954 40.0 38.0 41.0 34.0 41.0 30-31 38.31895437120101 40.0 38.0 41.0 34.0 41.0 32-33 38.21389032452289 40.0 37.0 41.0 34.0 41.0 34-35 38.06553538707303 40.0 37.0 41.0 34.0 41.0 36-37 37.88488912555098 40.0 37.0 41.0 33.0 41.0 38-39 37.74359228690406 40.0 37.0 41.0 33.0 41.0 40-41 37.551165995834054 40.0 37.0 41.0 33.0 41.0 42-43 37.31805470722637 40.0 36.0 41.0 33.0 41.0 44-45 36.97284128500554 40.0 36.0 41.0 31.5 41.0 46-47 36.52971327343694 39.5 36.0 41.0 30.5 41.0 48-49 36.11390407180606 39.0 35.0 41.0 29.5 41.0 50-51 35.83330375057208 39.0 35.0 41.0 28.5 41.0 52-53 35.581975571599855 39.0 35.0 40.5 28.0 41.0 54-55 35.343611602707 39.0 35.0 40.0 27.5 41.0 56-57 35.08540195141815 38.0 35.0 40.0 27.0 41.0 58-59 34.797396368977076 38.0 34.0 40.0 26.0 41.0 60-61 34.46587454473 38.0 34.0 40.0 26.0 41.0 62-63 34.09385305024371 37.0 34.0 40.0 25.5 41.0 64-65 33.69999686770763 36.5 33.5 39.5 24.5 41.0 66-67 33.2935741022067 36.0 33.0 39.0 22.5 40.5 68-69 32.85787397395673 35.5 32.5 39.0 22.0 40.0 70-71 32.44714604661199 35.0 32.0 38.0 20.5 40.0 72-73 31.988648050409026 35.0 32.0 37.5 19.5 39.5 74-75 31.51361068042091 35.0 31.0 37.0 18.0 39.0 76-77 30.275476412968388 33.5 29.5 35.5 17.5 38.0 78-79 30.869514790510546 34.0 31.0 36.0 17.0 38.5 80-81 30.678713811147134 34.0 31.0 36.0 12.5 37.5 82-83 30.342972963096376 34.0 31.0 35.0 7.5 37.0 84-85 29.996697171714008 34.0 30.5 35.0 6.0 36.5 86-87 29.60054745509946 34.0 30.0 35.0 2.0 36.0 88-89 29.152723615222246 34.0 29.5 35.0 2.0 36.0 90-91 28.671999394423473 34.0 29.0 35.0 2.0 35.0 92-93 28.331694970408538 34.0 29.0 35.0 2.0 35.0 94-95 27.930175982626217 34.0 28.5 35.0 2.0 35.0 96-97 27.464191807663326 33.0 27.0 35.0 2.0 35.0 98-99 26.746411350035412 33.0 26.0 35.0 2.0 35.0 100-101 24.31026748036662 31.0 19.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.014727465772772064 1101 2 0.029150347093931828 1101 3 -0.03934609751938467 1101 4 0.013071804969214895 1101 5 0.011383568787948661 1101 6 -0.08839902298706903 1101 7 -0.060462138274864685 1101 8 0.02347779828564711 1101 9 0.0899442587649446 1101 10-11 -0.03088238542803623 1101 12-13 -0.13145395722754216 1101 14-15 -0.07327650523202323 1101 16-17 0.04654007383035008 1101 18-19 0.0513073572050331 1101 20-21 0.06476667209256703 1101 22-23 -0.05782720635659899 1101 24-25 -0.03252490469126457 1101 26-27 -0.15598169289257413 1101 28-29 -0.16677862880023753 1101 30-31 -0.04909325839604861 1101 32-33 -0.20467157547532366 1101 34-35 -0.21127881105949342 1101 36-37 -0.15696408469044343 1101 38-39 -0.2707361226889944 1101 40-41 -0.023515278428341446 1101 42-43 -0.20835592766370326 1101 44-45 -0.01984423890240805 1101 46-47 -0.43136139373375215 1101 48-49 -0.5413629107820697 1101 50-51 -0.3826466146006098 1101 52-53 -0.5095837324039607 1101 54-55 -0.4874868568637609 1101 56-57 -0.3106266935846307 1101 58-59 -0.3096092651116962 1101 60-61 -0.44612040222310867 1101 62-63 -0.49281386675407646 1101 64-65 -0.35084124911809056 1101 66-67 -0.1491723916560872 1101 68-69 -0.051556519051239036 1101 70-71 -0.0881509632848676 1101 72-73 -0.08698052340437101 1101 74-75 -0.07014775025002251 1101 76-77 0.01526582090379236 1101 78-79 -0.14190315665769404 1101 80-81 -0.2919322591638469 1101 82-83 -0.2769069497702894 1101 84-85 -0.43995278619584965 1101 86-87 -0.4649836381470571 1101 88-89 -0.4556134544633821 1101 90-91 -0.6377708751191449 1101 92-93 -0.5987484948938899 1101 94-95 -0.47926534181325664 1101 96-97 -0.734749710772455 1101 98-99 -0.28526261053415425 1101 100-101 0.21577299708539144 1102 1 -0.04703348611398184 1102 2 -0.003945125347009082 1102 3 0.016848538307385752 1102 4 0.015552940139819782 1102 5 -0.0273508472225501 1102 6 -0.02113155251462473 1102 7 -0.1770139755452078 1102 8 -0.07161529554637269 1102 9 -0.12115728322980601 1102 10-11 -0.05702657834142144 1102 12-13 -0.04901260224065851 1102 14-15 -0.03413198423465502 1102 16-17 -0.02298810038225696 1102 18-19 -0.061999524356643576 1102 20-21 -0.1066828439835561 1102 22-23 -0.2445275016321915 1102 24-25 -0.196561773129595 1102 26-27 -0.06619433232826566 1102 28-29 -0.3274218784721512 1102 30-31 -0.1628820133173079 1102 32-33 0.1479059343671949 1102 34-35 0.18394961741819316 1102 36-37 0.12293256885286752 1102 38-39 0.23831181858397343 1102 40-41 0.24506760378951498 1102 42-43 0.2115095579005981 1102 44-45 -0.05260137472918558 1102 46-47 0.1500905829722825 1102 48-49 0.39230773390296747 1102 50-51 0.3143729474072572 1102 52-53 0.46917282927582704 1102 54-55 0.4371717307346543 1102 56-57 0.4278698615334804 1102 58-59 0.3505469848882967 1102 60-61 0.1474040400341039 1102 62-63 0.2652857067367336 1102 64-65 0.499178230868786 1102 66-67 0.659821210706113 1102 68-69 0.6353488287177882 1102 70-71 0.6601778588936114 1102 72-73 0.3101959037609845 1102 74-75 0.11988464804394283 1102 76-77 0.07847837831848992 1102 78-79 0.1550026012163208 1102 80-81 0.13710932548182342 1102 82-83 0.1416923366470364 1102 84-85 0.10731181354168129 1102 86-87 0.2508699953831268 1102 88-89 0.2287184845129957 1102 90-91 0.1063851288178661 1102 92-93 0.14906256973865695 1102 94-95 0.44744889703189017 1102 96-97 0.5123898069440855 1102 98-99 0.5895579685340842 1102 100-101 1.2255898852087483 1103 1 0.01605197000371561 1103 2 0.04891782411377221 1103 3 -0.05221385574386517 1103 4 0.06637595954105535 1103 5 0.05257022215031526 1103 6 -0.008000574148169903 1103 7 -0.014789061351784483 1103 8 -0.08378249743278587 1103 9 0.024174876810420187 1103 10-11 0.06736915666194676 1103 12-13 0.007347370179331847 1103 14-15 0.02249559805776613 1103 16-17 -0.27480899033269424 1103 18-19 -0.0670641126183753 1103 20-21 -0.19730830825334778 1103 22-23 -0.18687519256850038 1103 24-25 -0.12969263721425506 1103 26-27 -0.1918985938964397 1103 28-29 -0.2228749867465396 1103 30-31 -0.057645621422175 1103 32-33 0.02778148149129578 1103 34-35 -0.12202846333332218 1103 36-37 -0.161507202735919 1103 38-39 0.16701616776818895 1103 40-41 -0.12059985904033255 1103 42-43 -0.017121650934150523 1103 44-45 -0.14766573442732778 1103 46-47 -0.07220655731624959 1103 48-49 0.003655344842023567 1103 50-51 0.010842401787868994 1103 52-53 -0.006514670962260993 1103 54-55 -0.21170126028659553 1103 56-57 -0.3232015303045088 1103 58-59 -0.1789643842457096 1103 60-61 -0.29010095979447215 1103 62-63 -0.03730391876182182 1103 64-65 -0.1445101760048857 1103 66-67 -0.005414076464994366 1103 68-69 -0.14545392471161733 1103 70-71 -0.020928312704320717 1103 72-73 -0.13941725235937596 1103 74-75 -0.06299479608939862 1103 76-77 0.11317814099329482 1103 78-79 -0.2277442760336008 1103 80-81 -0.11850572009756632 1103 82-83 -0.1720835719884768 1103 84-85 -0.07088302930180035 1103 86-87 -0.11968682362117278 1103 88-89 -0.1454020218315506 1103 90-91 0.17093815366653686 1103 92-93 -6.037274168662066E-4 1103 94-95 -0.25276709556264976 1103 96-97 -0.19633541771488439 1103 98-99 0.09946143851035316 1103 100-101 0.8506198841802153 1104 1 -0.00254915694924307 1104 2 0.17296162160373996 1104 3 -0.052045239676246524 1104 4 0.08816371673392354 1104 5 0.06006616682716981 1104 6 0.03627928583645712 1104 7 0.05387609701925555 1104 8 0.01598698946212096 1104 9 -0.05947363339191725 1104 10-11 0.0681065851602014 1104 12-13 -0.01768251114910413 1104 14-15 0.02375168104247649 1104 16-17 0.031275061575442464 1104 18-19 -0.024159860932222443 1104 20-21 0.02978658630824782 1104 22-23 0.025904411290461837 1104 24-25 0.011676994818536457 1104 26-27 -0.06681962181414036 1104 28-29 -0.009448665564939063 1104 30-31 0.05501979307454263 1104 32-33 0.10842585099526758 1104 34-35 0.05304410560717088 1104 36-37 0.2140530721722982 1104 38-39 0.2733125905463041 1104 40-41 0.14538024853244735 1104 42-43 0.16872740566962818 1104 44-45 0.1297727954113128 1104 46-47 -0.11399772951807563 1104 48-49 -0.01459360931148268 1104 50-51 -0.1318270680319884 1104 52-53 -0.1175494186784718 1104 54-55 -0.38138238382455114 1104 56-57 -0.16733625240815542 1104 58-59 -0.40361968177836616 1104 60-61 -0.11612652967409076 1104 62-63 -0.3047930334207507 1104 64-65 -0.18689363882397458 1104 66-67 -0.14111479361687174 1104 68-69 -0.04245725434535785 1104 70-71 -0.07909765446133576 1104 72-73 -0.10118089105142758 1104 74-75 -0.2214191963284513 1104 76-77 0.1358004410018303 1104 78-79 -0.05628734783416789 1104 80-81 -0.16232135230110245 1104 82-83 0.038434655621870206 1104 84-85 -0.2104491097252641 1104 86-87 -0.02765980236274146 1104 88-89 0.07074990337975251 1104 90-91 -0.3575931790407125 1104 92-93 -0.162910565972318 1104 94-95 -0.17957171436227526 1104 96-97 -0.1771057156744149 1104 98-99 -0.02571144631847133 1104 100-101 0.1332438916932297 1105 1 -0.019248693906881442 1105 2 -0.05392484726952773 1105 3 -0.0793690263337723 1105 4 -0.1164152577130011 1105 5 -0.18596367368046174 1105 6 -0.15760684004246173 1105 7 -0.10920484964512411 1105 8 -0.04304233655400225 1105 9 -0.12688488360629435 1105 10-11 -0.15080312157964215 1105 12-13 -0.15981507846174026 1105 14-15 -0.2246598264176427 1105 16-17 -0.11220590223864946 1105 18-19 -0.1716924453945765 1105 20-21 -0.19928861520092056 1105 22-23 -0.15665647020499307 1105 24-25 -0.2481905236174171 1105 26-27 -0.37433119787313274 1105 28-29 -0.13704899140956428 1105 30-31 -0.24588056706951278 1105 32-33 -0.3836188240175957 1105 34-35 -0.3117518127601784 1105 36-37 -0.268464935030579 1105 38-39 -0.43683146707675036 1105 40-41 -0.6413158584817893 1105 42-43 -0.4547419820854799 1105 44-45 -0.5924382318567396 1105 46-47 -0.6241589368581515 1105 48-49 -0.5293423659569925 1105 50-51 -0.9498064026229684 1105 52-53 -1.0288682319180538 1105 54-55 -0.7082431281222696 1105 56-57 -0.5814676192406054 1105 58-59 -0.6718048129737042 1105 60-61 -0.6886419644194177 1105 62-63 -0.4364256864819751 1105 64-65 -0.40948624729593064 1105 66-67 -0.7919915729000238 1105 68-69 -0.5497115846489109 1105 70-71 -0.41915724972801627 1105 72-73 -0.4737220503723023 1105 74-75 -0.37870353863680606 1105 76-77 0.07400572311467712 1105 78-79 -0.30301603931075505 1105 80-81 -0.3710784265593787 1105 82-83 -0.348394361699345 1105 84-85 -0.33565705004424373 1105 86-87 -0.266879061494965 1105 88-89 -0.22388437890458945 1105 90-91 0.0910541370285749 1105 92-93 0.26282258443070106 1105 94-95 0.15212739385244234 1105 96-97 4.946101702074657E-4 1105 98-99 -0.03907965074309416 1105 100-101 0.48020410434972405 1106 1 -0.17661567350801732 1106 2 -0.08510902292201195 1106 3 -0.08994044560551373 1106 4 -0.0013944031647596944 1106 5 -0.22405054205734132 1106 6 -0.06597702338579126 1106 7 -0.08692744927964924 1106 8 -0.19714745091690133 1106 9 -0.207017962128198 1106 10-11 -0.2488473973897598 1106 12-13 -0.2514262957442881 1106 14-15 -0.3560341806706262 1106 16-17 -0.1847853099415815 1106 18-19 -0.32945233677423147 1106 20-21 -0.13762006534762605 1106 22-23 -0.2133171385735082 1106 24-25 -0.142426345082022 1106 26-27 -0.3121662772227154 1106 28-29 -0.23996630324202073 1106 30-31 -0.17009044240721494 1106 32-33 -0.27986221542747813 1106 34-35 -0.2553689725427546 1106 36-37 -0.1958646030317439 1106 38-39 -0.031467407577352446 1106 40-41 -0.0805110170647012 1106 42-43 -0.3036011429747134 1106 44-45 -0.28831637805711097 1106 46-47 -0.2338964066922884 1106 48-49 -0.13986333442640841 1106 50-51 -0.20915491304558742 1106 52-53 -0.3910131582412859 1106 54-55 -0.29193449940762406 1106 56-57 -0.32616447269148807 1106 58-59 -0.5386077200558859 1106 60-61 -0.5749207411385839 1106 62-63 -0.4955426346966618 1106 64-65 -0.3411939953302152 1106 66-67 -0.4276437832031448 1106 68-69 -0.6812789074084975 1106 70-71 -0.4751936766022524 1106 72-73 -0.6910661992416927 1106 74-75 -0.4442942315099856 1106 76-77 -0.5522026549734029 1106 78-79 -0.5451066554615203 1106 80-81 -0.6418562523855371 1106 82-83 -0.7743432785182911 1106 84-85 -0.8687929975196127 1106 86-87 -0.846098445762685 1106 88-89 -0.7317984125973567 1106 90-91 -0.35092627783046737 1106 92-93 -0.20313009876151256 1106 94-95 -0.16639153798550055 1106 96-97 -0.14339828533385557 1106 98-99 -0.23516355251980414 1106 100-101 0.896061698241688 1107 1 -0.15443446536758643 1107 2 -0.12807041195522828 1107 3 -0.11555701391593232 1107 4 -0.17102209697568327 1107 5 -0.10662274536655758 1107 6 -0.02115707606648698 1107 7 -0.16788717879350656 1107 8 -0.06960744280025466 1107 9 -0.13797730390388807 1107 10-11 -0.09954881573597874 1107 12-13 -0.11285125936151985 1107 14-15 -0.04179436719852703 1107 16-17 0.04787697786870382 1107 18-19 -0.06074461639031625 1107 20-21 -0.049178281648657673 1107 22-23 -0.18603071121883374 1107 24-25 -0.038848555758256964 1107 26-27 -0.17337410746576865 1107 28-29 0.11673471702633975 1107 30-31 0.08400730380006394 1107 32-33 0.0038031510238667465 1107 34-35 0.09579126930246673 1107 36-37 0.11328679321339763 1107 38-39 0.1066475128458535 1107 40-41 0.19614533576669402 1107 42-43 0.15794944780900977 1107 44-45 0.33655728392309214 1107 46-47 0.5763445338054112 1107 48-49 0.5088833665680141 1107 50-51 0.2286350827926853 1107 52-53 0.452109271377779 1107 54-55 0.12206646735287308 1107 56-57 0.4367775811317429 1107 58-59 0.2697798319979725 1107 60-61 0.2948599800027125 1107 62-63 0.2047693652826581 1107 64-65 0.4283163430618089 1107 66-67 0.2705796005103025 1107 68-69 0.46281493556788433 1107 70-71 0.5893776530036234 1107 72-73 0.5931691423168601 1107 74-75 0.5815291304901251 1107 76-77 0.719442828264679 1107 78-79 0.5684075706518676 1107 80-81 0.5964747674433895 1107 82-83 0.5162781106700187 1107 84-85 0.651581350757148 1107 86-87 0.5936470429037257 1107 88-89 0.6007348876489864 1107 90-91 0.789721865291444 1107 92-93 0.9897541303762338 1107 94-95 1.0530398310662683 1107 96-97 1.2082181950892448 1107 98-99 1.2778398931800545 1107 100-101 1.6650033539870641 1108 1 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1.0960827754728903 2315 80-81 1.351229789805224 2315 82-83 1.6803994827898485 2315 84-85 1.3521165866563827 2315 86-87 1.1173093708735635 2315 88-89 1.6462577869455508 2315 90-91 1.4480592365647063 2315 92-93 1.473919836291678 2315 94-95 1.5359301049221408 2315 96-97 1.4412463158598499 2315 98-99 1.5455135385208258 2315 100-101 1.1502996830819505 2316 1 -0.3148164689373303 2316 2 -0.2793280284334898 2316 3 0.012753717881459181 2316 4 0.026026710032503786 2316 5 0.11103414895672614 2316 6 0.1344550118230572 2316 7 0.1677815326112082 2316 8 0.18046949132362045 2316 9 0.1445658304948978 2316 10-11 0.22139213988841533 2316 12-13 0.24817750374293013 2316 14-15 0.4442933787342227 2316 16-17 0.466280646087732 2316 18-19 0.4665977712345679 2316 20-21 0.4976104880208325 2316 22-23 0.35405966073201256 2316 24-25 0.37505190174332625 2316 26-27 0.3724581145968813 2316 28-29 0.5977832778453305 2316 30-31 0.33562292040141983 2316 32-33 0.26640239604366656 2316 34-35 0.1400694220628651 2316 36-37 0.3842410840859287 2316 38-39 0.4367154200325203 2316 40-41 0.049475557542130844 2316 42-43 0.2567020892139311 2316 44-45 0.1424217157389407 2316 46-47 0.3777185772578022 2316 48-49 -0.021015798440302547 2316 50-51 0.13173398930234015 2316 52-53 0.4826367317310627 2316 54-55 0.7480666109632423 2316 56-57 0.6329057431242333 2316 58-59 0.5921081663228946 2316 60-61 0.7485328150764659 2316 62-63 0.6958398779716575 2316 64-65 0.8797016701857103 2316 66-67 1.0210873657278725 2316 68-69 1.0316119936069867 2316 70-71 1.293919843677159 2316 72-73 1.3529423405196823 2316 74-75 1.6285435735896385 2316 76-77 1.03157482670548 2316 78-79 1.0149896439752162 2316 80-81 1.154385646954296 2316 82-83 1.4924182743858303 2316 84-85 1.3016413593737717 2316 86-87 1.3494530047854312 2316 88-89 1.5461501267602458 2316 90-91 1.2742478485095496 2316 92-93 1.3807230900217249 2316 94-95 1.4092499535453946 2316 96-97 1.6501560118013856 2316 98-99 2.033409923748547 2316 100-101 1.6140703995115508 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 4.0 5 12.0 6 26.0 7 69.0 8 158.0 9 295.0 10 463.0 11 775.0 12 1244.0 13 1839.0 14 2486.0 15 3500.0 16 4888.0 17 7150.0 18 6626.0 19 3995.0 20 3251.0 21 3111.0 22 3220.0 23 3276.0 24 3793.0 25 4248.0 26 5224.0 27 6476.0 28 7891.0 29 10061.0 30 12321.0 31 15450.0 32 20046.0 33 26159.0 34 36545.0 35 55176.0 36 83695.0 37 126998.0 38 103891.0 39 10295.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.01092089317305 19.017537505746006 15.137764838624301 21.833776762456644 2 16.000445481581252 20.478579470607787 40.08690371159244 23.434071336218523 3 19.723522993636227 24.432576536693933 30.56142860374587 25.282471865923966 4 12.776968602249333 16.941699338216228 36.66383020191105 33.61750185762339 5 13.145241704066308 37.83387772361911 33.93010341965269 15.090777152661886 6 31.827222752971764 36.59091739622976 16.799180035464513 14.782679815333962 7 28.39353425248713 33.650738959974525 19.824974463116387 18.130752324421962 8 27.3805857038696 34.67221430448318 18.90460255560254 19.04259743604468 9 26.76717844843638 16.2315390518551 19.898931366253723 37.102351133454796 10-11 25.358255939609403 26.941194691112468 26.466304364849414 21.234245004428715 12-13 26.39886882749619 24.2458994996737 27.750358929736784 21.604872743093324 14-15 22.9148938761944 25.295436076003337 26.724109428374042 25.06556061942822 16-17 21.624215404265833 28.60505099545992 27.557386206428507 22.21334739384574 18-19 21.569487295944203 26.021953889176014 31.31448389392666 21.09407492095312 20-21 22.079615911349165 25.052074360620818 31.93902818889115 20.929281539138863 22-23 21.751969874150795 25.197944509410846 31.88933901325315 21.16074660318521 24-25 21.640810573300502 25.263157894736842 31.953171903141886 21.14285962882077 26-27 21.27223607105102 25.720276860564773 31.74595302419267 21.261534044191542 28-29 21.267968749320232 25.834365132469088 31.85383633504947 21.04382978316121 30-31 21.332097814785328 25.647363864562806 31.685743247064558 21.334795073587312 32-33 21.055095282593676 25.593264875343465 31.759008385842733 21.592631456220126 34-35 21.53990453468927 25.590219591096634 31.745435118914 21.1244407553001 36-37 21.547039200638988 25.438912468089768 31.563849169681497 21.45019916158974 38-39 21.350947349370756 25.75236315291235 31.563404889185875 21.333284608531017 40-41 21.454897600141997 25.813134397964703 31.549579837782062 21.182388164111238 42-43 21.443184497988543 25.705402260942055 31.315813976558672 21.535599264510726 44-45 21.57702631567499 26.024458046749587 31.038575151285762 21.35994048628966 46-47 21.821114782853833 26.417666825021445 30.291007362627226 21.470211029497495 48-49 21.925611536580824 26.721847217219256 29.90991179116659 21.442629455033334 50-51 21.94501434763921 26.778924544817013 29.75277512403008 21.523285983513702 52-53 22.26555226664857 26.717844843637707 29.553004477437923 21.463598412275804 54-55 21.84304082943102 26.959031355986767 29.60338217969265 21.594545634889563 56-57 21.841387675125596 27.247289261979713 29.358889358732743 21.552433704161945 58-59 21.861138518669332 26.6272693893248 29.757386554460997 21.754205537544873 60-61 21.768735893808326 26.720107054792496 30.005794740881115 21.505362310518063 62-63 21.645184361508303 26.828519173979696 30.159625099406778 21.36667136510522 64-65 21.581407408567514 26.79728325841934 29.95124064880216 21.67006868421098 66-67 21.54381990014948 27.138442102185817 29.654194922554073 21.66354307511063 68-69 21.796752508879177 26.80163366448624 29.81864027188298 21.5829735547516 70-71 21.6604977908638 27.107032170382784 29.530643390254045 21.70182664849937 72-73 21.641094979805413 27.032466210396077 29.29398130021456 22.032457509583946 74-75 21.74402558734832 27.01584765922051 29.266747758235756 21.97337899519541 76-77 21.63691858998119 26.95311480373578 29.449116780560296 21.960849825722732 78-79 21.775261502908684 27.015760651099175 29.211062560579403 21.997915285412738 80-81 21.650423686415497 27.41628288801088 29.064914205567305 21.868379220006318 82-83 21.67701776886038 27.362148203189037 29.05437504459182 21.906458983358768 84-85 21.430703144237103 27.275432455501523 29.02996496155516 22.263899438706215 86-87 21.576099913165894 27.230409686440133 28.87695137464131 22.316539025752665 88-89 21.45829091687418 27.15810593760822 28.912624704389906 22.470978441127695 90-91 21.32837924853379 26.823758723307982 29.26941751796871 22.57844451018952 92-93 21.233723575962447 26.542241480572702 29.638514380680164 22.585520562784687 94-95 21.151065240429542 26.564797558204084 29.542563502877357 22.741573698489017 96-97 21.258868302767382 26.55922903843845 29.413356442690358 22.76854621610381 98-99 20.938591408122033 26.661289564767976 29.339660563917036 23.060458463192955 100-101 20.6966194950083 26.71946829422376 29.553509506990473 23.030402703777476 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 288.0 1 215.5 2 240.5 3 374.0 4 282.5 5 196.5 6 468.5 7 1161.5 8 1893.0 9 2151.0 10 1713.5 11 1186.0 12 1121.5 13 1150.0 14 1147.5 15 1226.5 16 1348.5 17 1414.0 18 1483.5 19 1569.0 20 1662.0 21 1802.5 22 2049.5 23 2316.0 24 2666.5 25 3223.0 26 3928.0 27 4746.0 28 5505.5 29 6293.5 30 7062.0 31 8093.5 32 9559.5 33 10513.5 34 11230.5 35 11992.5 36 12978.0 37 14170.5 38 15215.0 39 15734.0 40 16118.0 41 17323.0 42 18108.5 43 18654.5 44 19457.0 45 20184.5 46 20762.0 47 21217.0 48 21820.0 49 21500.5 50 20705.5 51 19975.5 52 19308.5 53 19320.0 54 18962.0 55 17232.0 56 15501.0 57 14152.5 58 12870.0 59 11528.0 60 9492.0 61 7556.5 62 6082.5 63 4478.5 64 3020.5 65 2172.5 66 1563.5 67 1182.5 68 904.0 69 634.0 70 464.5 71 312.5 72 209.0 73 182.5 74 153.5 75 114.0 76 79.5 77 47.5 78 25.5 79 18.5 80 13.0 81 6.5 82 6.0 83 4.5 84 3.0 85 2.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06038363620860371 2 0.0 3 0.0 4 0.0 5 0.001740162426760914 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005916552250987107 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.220487280282742E-4 24-25 6.960649707043656E-4 26-27 2.610243640141371E-4 28-29 0.0026972517614794163 30-31 2.610243640141371E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 5.220487280282742E-4 40-41 0.0 42-43 0.0131382263220449 44-45 2.610243640141371E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 8.70081213380457E-5 82-83 3.480324853521828E-4 84-85 7.830730920424111E-4 86-87 0.0 88-89 0.0 90-91 0.008526795891128477 92-93 0.002871268004155508 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.009657901468523071 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 574659.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.67050431959356 #Duplication Level Percentage of deduplicated Percentage of total 1 84.37537472971422 57.097241601144525 2 9.579761356820296 12.965345645547663 3 2.5857151221458787 5.249299390272332 4 1.1507743126325845 3.114939123755225 5 0.6037960976616071 2.0429593217481763 6 0.3727332905743037 1.5133829849918845 7 0.25366215369771444 1.2015812107263013 8 0.18164185740422367 0.9833436876873212 9 0.13046469023922125 0.7945750245948893 >10 0.7123242336378679 8.356519504915154 >50 0.03333668314206691 1.5462600257758408 >100 0.018606506427426832 2.3944106992373593 >500 7.752711011419984E-4 0.3378616324075357 >1k 7.752711011419984E-4 1.5126827744954365 >5k 2.5842370038066614E-4 0.8895973727003799 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5087 0.8852206264932768 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4408 0.7670635977162108 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3114 0.5418865796933485 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1128 0.1962903217386311 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 881 0.15330830979763652 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.740162426760914E-4 2 0.0 0.0 0.0 0.0 1.740162426760914E-4 3 0.0 0.0 0.0 0.0 1.740162426760914E-4 4 0.0 0.0 0.0 0.0 1.740162426760914E-4 5 0.0 0.0 0.0 0.0 1.740162426760914E-4 6 0.0 0.0 0.0 0.0 1.740162426760914E-4 7 0.0 0.0 0.0 0.0 1.740162426760914E-4 8 0.0 0.0 0.0 0.0 1.740162426760914E-4 9 0.0 0.0 0.0 0.0 1.740162426760914E-4 10-11 0.0 0.0 0.0 0.0 1.740162426760914E-4 12-13 0.0 0.0 0.0 0.0 1.740162426760914E-4 14-15 0.0 0.0 0.0 0.0 1.740162426760914E-4 16-17 8.70081213380457E-5 0.0 0.0 1.740162426760914E-4 1.740162426760914E-4 18-19 1.740162426760914E-4 0.0 0.0 1.740162426760914E-4 1.740162426760914E-4 20-21 1.740162426760914E-4 0.0 0.0 3.480324853521828E-4 1.740162426760914E-4 22-23 1.740162426760914E-4 0.0 0.0 6.090568493663199E-4 3.480324853521828E-4 24-25 1.740162426760914E-4 0.0 0.0 8.70081213380457E-4 5.220487280282742E-4 26-27 1.740162426760914E-4 0.0 0.0 0.0012181136987326397 5.220487280282742E-4 28-29 1.740162426760914E-4 0.0 0.0 0.0023492192761272335 5.220487280282742E-4 30-31 1.740162426760914E-4 0.0 0.0 0.005742536008311016 5.220487280282742E-4 32-33 1.740162426760914E-4 0.0 0.0 0.011398063895283986 8.70081213380457E-4 34-35 1.740162426760914E-4 0.0 0.0 0.016009494326200406 8.70081213380457E-4 36-37 1.740162426760914E-4 0.0 0.0 0.020707932878454877 8.70081213380457E-4 38-39 1.740162426760914E-4 0.0 0.0 0.028190631313526802 8.70081213380457E-4 40-41 1.740162426760914E-4 0.0 0.0 0.03384615920049977 8.70081213380457E-4 42-43 1.740162426760914E-4 0.0 0.0 0.04942061292000995 8.70081213380457E-4 44-45 1.740162426760914E-4 0.0 0.0 0.06934547270642241 8.70081213380457E-4 46-47 2.610243640141371E-4 0.0 0.0 0.08770418630875006 8.70081213380457E-4 48-49 3.480324853521828E-4 0.0 0.0 0.11093535470600827 8.70081213380457E-4 50-51 3.480324853521828E-4 0.0 0.0 0.14208426214502862 8.70081213380457E-4 52-53 3.480324853521828E-4 0.0 0.0 0.20151080901891383 8.70081213380457E-4 54-55 3.480324853521828E-4 0.0 0.0 0.24893023514814872 8.70081213380457E-4 56-57 3.480324853521828E-4 0.0 0.0 0.3305438529632356 8.70081213380457E-4 58-59 3.480324853521828E-4 0.0 0.0 0.4247736483723391 8.70081213380457E-4 60-61 3.480324853521828E-4 0.0 0.0 0.5205695899655274 8.70081213380457E-4 62-63 3.480324853521828E-4 0.0 0.0 0.6526479181566808 8.70081213380457E-4 64-65 3.480324853521828E-4 0.0 0.0 0.7286060080847947 8.70081213380457E-4 66-67 5.220487280282742E-4 0.0 0.0 0.8888749675894748 8.70081213380457E-4 68-69 5.220487280282742E-4 0.0 0.0 1.090907825336417 8.70081213380457E-4 70-71 5.220487280282742E-4 0.0 0.0 1.2553531746653233 8.70081213380457E-4 72-73 5.220487280282742E-4 0.0 0.0 1.519422822926292 8.70081213380457E-4 74-75 5.220487280282742E-4 0.0 0.0 1.780273170697753 9.570893347185026E-4 76-77 5.220487280282742E-4 0.0 0.0 1.961424079323564 0.0010440974560565483 78-79 5.220487280282742E-4 0.0 0.0 2.1705916030202257 0.0010440974560565483 80-81 5.220487280282742E-4 0.0 0.0 2.517144950309662 0.0010440974560565483 82-83 5.220487280282742E-4 0.0 0.0 2.8280249678504994 0.0010440974560565483 84-85 6.090568493663199E-4 0.0 0.0 3.2574970547750928 0.0010440974560565483 86-87 6.960649707043656E-4 0.0 0.0 3.787811554330481 0.0010440974560565483 88-89 6.960649707043656E-4 0.0 0.0 4.458122121118786 0.0010440974560565483 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACCCG 20 0.0020740523 71.24563 5 GGTATCA 1890 0.0 58.34391 1 GTATCAA 3235 0.0 39.081993 1 GTGGTAT 800 0.0 29.706358 1 TATCAAC 4245 0.0 29.538824 2 ATCAACG 4280 0.0 29.29727 3 TGGTATC 795 0.0 29.274933 2 CGTACAC 65 0.005843425 29.228975 3 TCAACGC 4305 0.0 29.127132 4 GTATAGG 165 2.6229827E-9 28.806166 1 CAACGCA 4420 0.0 28.26184 5 AACGCAG 4505 0.0 27.834032 6 CGCAGAG 5235 0.0 24.136244 8 ACGCAGA 5250 0.0 24.067284 7 GAGTACT 3910 0.0 22.475002 12-13 GTACATG 2570 0.0 22.378017 1 TACATGG 2605 0.0 22.244316 2 GCAGAGT 5615 0.0 22.164415 9 GTAAGGT 215 4.4146873E-8 22.091667 4 GTATTAG 110 0.0028650565 21.604624 1 >>END_MODULE