FastQCFastQC Report
Thu 26 May 2016
SRR937207_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937207_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1407983
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53720.3815386975552972No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT43220.3069639335134018No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39190.27834142883827434No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26960.19147958462566664No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT25770.18302777803425185No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22150.15731724033599837No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT19040.135228905462637No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC16350.11612356115095139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG62400.027.2533192
GTACATG63250.027.126871
ACATGGG64300.024.969583
GTATCAA103750.022.584571
CATGGGA42550.022.5545754
GAGTACT48800.020.4407712-13
GTATTAG5950.019.9699081
AGAGTAC84200.019.68948210-11
AGTACTT51250.019.60262712-13
GTGTAGC8600.019.3429451
TCAACGC119150.019.2989714
ATCAACG119500.019.199273
CAACGCA119200.019.1733535
TATCAAC120650.019.0563182
AACGCAG121450.018.8134546
CATGGGG22800.018.7538534
GTACTTT52500.018.72741714-15
ATGGGAT19300.018.7105065
GTAGCCC7000.018.321933
GGTATCA79000.018.0487541
Produced by FastQC (version 0.11.5)