##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937207_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1407983 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.735680757509147 33.0 31.0 34.0 30.0 34.0 2 31.84720199036494 33.0 31.0 34.0 30.0 34.0 3 31.889902079783635 33.0 31.0 34.0 30.0 34.0 4 35.50648409817448 37.0 35.0 37.0 33.0 37.0 5 35.520398328673004 37.0 35.0 37.0 33.0 37.0 6 35.561805788848304 37.0 35.0 37.0 33.0 37.0 7 35.50357426190515 37.0 35.0 37.0 33.0 37.0 8 35.48786668589038 37.0 35.0 37.0 33.0 37.0 9 37.08478937600809 39.0 37.0 39.0 33.0 39.0 10-11 36.97389776723156 39.0 37.0 39.0 33.0 39.0 12-13 36.83172062446776 39.0 37.0 39.0 33.0 39.0 14-15 37.93055988602135 40.0 38.0 41.0 33.0 41.0 16-17 37.85266654497959 40.0 38.0 41.0 33.0 41.0 18-19 37.781507660248735 40.0 38.0 41.0 33.0 41.0 20-21 37.6545011551986 40.0 37.0 41.0 32.0 41.0 22-23 37.73408095126149 40.0 37.5 41.0 32.5 41.0 24-25 37.702993573075815 40.0 37.0 41.0 32.0 41.0 26-27 37.615157995515574 40.0 37.0 41.0 32.0 41.0 28-29 37.488121305441894 40.0 37.0 41.0 32.0 41.0 30-31 37.24796783767986 39.5 37.0 41.0 31.5 41.0 32-33 37.01523526917583 39.0 36.0 41.0 31.0 41.0 34-35 37.01355201021603 39.0 36.0 41.0 31.0 41.0 36-37 36.87703970857603 39.0 36.0 41.0 30.5 41.0 38-39 36.737158403190946 39.0 36.0 41.0 30.0 41.0 40-41 36.5310330451433 39.0 35.5 41.0 30.0 41.0 42-43 36.26556748199374 39.0 35.0 40.5 30.0 41.0 44-45 36.01814617079894 39.0 35.0 40.0 29.5 41.0 46-47 35.63630136159314 39.0 35.0 40.0 27.5 41.0 48-49 35.30069184073955 38.5 35.0 40.0 26.5 41.0 50-51 34.351417595240854 37.5 33.5 39.5 25.0 40.5 52-53 34.4712269253251 38.0 34.0 39.5 25.5 40.5 54-55 34.78302792008142 38.0 34.0 40.0 25.5 41.0 56-57 34.51189751580807 38.0 34.0 40.0 24.0 41.0 58-59 34.238865810169585 38.0 34.0 40.0 23.0 41.0 60-61 33.882045095714936 38.0 33.0 40.0 22.5 41.0 62-63 33.49867789596891 37.0 33.0 40.0 21.5 41.0 64-65 33.072451513974244 36.5 32.0 39.5 20.0 41.0 66-67 32.60936957335423 36.0 31.5 39.0 18.5 40.5 68-69 32.0853323513139 35.5 31.0 39.0 15.0 40.0 70-71 31.589350510624065 35.0 31.0 38.0 10.5 40.0 72-73 31.066694697308137 35.0 30.0 37.5 7.5 39.5 74-75 30.480776756537544 34.5 29.5 37.0 6.0 39.0 76-77 29.92875446649569 34.0 29.0 36.5 2.0 39.0 78-79 29.342237441787294 34.0 29.0 36.0 2.0 38.5 80-81 28.701209815743514 34.0 27.5 35.5 2.0 37.0 82-83 28.100402845772997 33.5 26.5 35.0 2.0 37.0 84-85 27.46465049649037 33.0 26.0 35.0 2.0 36.0 86-87 26.818579840807736 32.5 25.0 35.0 2.0 36.0 88-89 26.191740596299812 32.0 24.5 35.0 2.0 35.5 90-91 25.555477942560387 32.0 21.5 34.0 2.0 35.0 92-93 24.811530394898234 31.0 18.5 34.0 2.0 35.0 94-95 23.95696609973274 31.0 9.0 34.0 2.0 35.0 96-97 23.025853295103705 31.0 2.0 34.0 2.0 35.0 98-99 21.78408617149497 30.0 2.0 34.0 2.0 35.0 100-101 18.67593500773802 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12957512938156768 1101 2 0.2051321237143391 1101 3 0.11122595147519831 1101 4 0.0873957051528862 1101 5 -0.008687701765829559 1101 6 -0.028160884529107477 1101 7 0.04536204019791512 1101 8 -0.021793549638431386 1101 9 0.1599995248310364 1101 10-11 0.2725302709494457 1101 12-13 0.3646380283777191 1101 14-15 0.49136277936473505 1101 16-17 0.3666468978987396 1101 18-19 0.3856308522333691 1101 20-21 0.4266796233757688 1101 22-23 0.5106278337468453 1101 24-25 0.3472071872855551 1101 26-27 0.2762038055359497 1101 28-29 0.27813428758944525 1101 30-31 0.30318347660954714 1101 32-33 0.2061419795581827 1101 34-35 0.1670247514179266 1101 36-37 0.21253458385250923 1101 38-39 0.19301134110752827 1101 40-41 0.44075201607810044 1101 42-43 0.48891182644606346 1101 44-45 0.39744510474561423 1101 46-47 0.5806339463855821 1101 48-49 0.39316015863190046 1101 50-51 0.42879180187593846 1101 52-53 0.4704662146209415 1101 54-55 0.46462116349665195 1101 56-57 0.785052637745558 1101 58-59 0.8339373964381664 1101 60-61 0.8941880024462776 1101 62-63 0.8547643151266655 1101 64-65 0.8633063060628885 1101 66-67 0.9801342758076714 1101 68-69 1.159969977573411 1101 70-71 1.0089182256354547 1101 72-73 0.923369521846702 1101 74-75 1.080593202461845 1101 76-77 1.001346554173388 1101 78-79 1.2406963967373699 1101 80-81 1.353259673125418 1101 82-83 1.0643497395862092 1101 84-85 1.2815189085895717 1101 86-87 1.2614517017209579 1101 88-89 1.3153515126167434 1101 90-91 1.3770758345994096 1101 92-93 1.5755944778866855 1101 94-95 1.6857617678115666 1101 96-97 1.5744790648710207 1101 98-99 1.6526566528574378 1101 100-101 1.793366974265826 1102 1 0.1413371293181207 1102 2 0.02063924595030997 1102 3 0.003497770262672617 1102 4 0.06363852739837483 1102 5 0.035065669002861455 1102 6 0.007637472122013378 1102 7 -0.010529270356954612 1102 8 -0.0880287579122836 1102 9 0.10295501482183766 1102 10-11 0.09464727231361536 1102 12-13 0.058997344389268846 1102 14-15 0.27320187076411884 1102 16-17 0.45259832710466696 1102 18-19 0.3640143108799876 1102 20-21 0.21353600483575264 1102 22-23 0.2153080469380555 1102 24-25 0.39037919959481115 1102 26-27 0.36870087098439086 1102 28-29 0.39026429615516633 1102 30-31 0.2996279104790247 1102 32-33 0.27947007828601045 1102 34-35 0.31965236475505776 1102 36-37 0.46928008685051026 1102 38-39 0.4369614061435385 1102 40-41 0.52679782680746 1102 42-43 0.608584425665633 1102 44-45 0.6840207210014455 1102 46-47 0.7950529051749626 1102 48-49 0.6781162196705708 1102 50-51 0.6108164838340073 1102 52-53 0.6172390010173103 1102 54-55 0.9394871258709472 1102 56-57 0.9073028836133901 1102 58-59 0.8505937525182432 1102 60-61 0.8549697035344437 1102 62-63 0.7907284342853202 1102 64-65 0.7421648448148304 1102 66-67 0.9811042640694652 1102 68-69 1.186298456999829 1102 70-71 0.9590784362886708 1102 72-73 0.9676844230236945 1102 74-75 1.1177739708384884 1102 76-77 1.1960492920306862 1102 78-79 1.009295108707171 1102 80-81 0.8960256069473154 1102 82-83 1.2476561067699912 1102 84-85 1.365700089389673 1102 86-87 1.5181258237348487 1102 88-89 1.4009793480280557 1102 90-91 1.5920785312143657 1102 92-93 1.7159842180599014 1102 94-95 1.82890522772718 1102 96-97 1.6959940866025676 1102 98-99 1.985462560030431 1102 100-101 2.2438951935482088 1103 1 0.24361393927759778 1103 2 0.2549919217242156 1103 3 0.08147955578299815 1103 4 0.08799621633182397 1103 5 0.18877749588175163 1103 6 0.21100882191522174 1103 7 0.09552829742514035 1103 8 0.19418079215665074 1103 9 0.1933924129920328 1103 10-11 0.30617440603964496 1103 12-13 0.31488989566970815 1103 14-15 0.2876815465797975 1103 16-17 0.2869259370808521 1103 18-19 0.30766117556645867 1103 20-21 0.29119611261867817 1103 22-23 0.25202526296344985 1103 24-25 0.20668136778631663 1103 26-27 0.2548248631304304 1103 28-29 0.10129429155976766 1103 30-31 0.34474530196501263 1103 32-33 0.3777603819999342 1103 34-35 0.4550933139132667 1103 36-37 0.4672288690705031 1103 38-39 0.38681205576546773 1103 40-41 0.43890147388102463 1103 42-43 0.24772780202858513 1103 44-45 0.5837878179642999 1103 46-47 0.47738882864072707 1103 48-49 0.5184355997098393 1103 50-51 0.5484089139630939 1103 52-53 0.4476312807516081 1103 54-55 0.8171931876163612 1103 56-57 0.72501082529363 1103 58-59 0.8729712434318913 1103 60-61 0.9509059110792535 1103 62-63 0.9850938518397285 1103 64-65 0.8209926010081787 1103 66-67 1.1291292880908514 1103 68-69 0.9360227332249167 1103 70-71 0.9385182157096494 1103 72-73 0.8973142847440414 1103 74-75 0.8824040414404841 1103 76-77 0.8244718178275718 1103 78-79 1.0310950418650435 1103 80-81 1.1947482956718822 1103 82-83 1.234463141903884 1103 84-85 1.2625010669557817 1103 86-87 1.4249687865121423 1103 88-89 1.5793016106340652 1103 90-91 1.4502625141696228 1103 92-93 1.5368807380916536 1103 94-95 1.4850191935541446 1103 96-97 1.6061206688810472 1103 98-99 1.726254012593408 1103 100-101 1.3686393135298296 1104 1 0.1015495026549047 1104 2 0.2914508617358642 1104 3 0.1064810329128072 1104 4 0.14169705956192757 1104 5 0.20247639774125048 1104 6 0.10299180537980845 1104 7 0.1479090086420385 1104 8 0.27276958453972355 1104 9 0.21609885243393023 1104 10-11 0.25903265222232363 1104 12-13 0.21992922952637173 1104 14-15 0.4089532709389445 1104 16-17 0.22221372417158136 1104 18-19 0.4452163569157932 1104 20-21 0.2984610590621699 1104 22-23 0.31100473507946447 1104 24-25 0.23270818402292548 1104 26-27 0.31890887383329414 1104 28-29 0.32725885732580906 1104 30-31 0.12784102841745693 1104 32-33 0.1986079590850025 1104 34-35 0.4028324612295364 1104 36-37 0.569194295792208 1104 38-39 0.5091124955281074 1104 40-41 0.5976604316182303 1104 42-43 0.5011659271710229 1104 44-45 0.41220146495560783 1104 46-47 0.45919143345363267 1104 48-49 0.6073296937228179 1104 50-51 0.46792095468122596 1104 52-53 0.6154802843220466 1104 54-55 0.7913963815930529 1104 56-57 0.854305052670334 1104 58-59 0.8490487041191344 1104 60-61 0.979271234823905 1104 62-63 0.8817122796671697 1104 64-65 1.1581867296281985 1104 66-67 1.2183472955693517 1104 68-69 1.1348408183582421 1104 70-71 1.2090501090524413 1104 72-73 1.3297218797234898 1104 74-75 0.9802039620975087 1104 76-77 1.2432011907648644 1104 78-79 1.1606272981597243 1104 80-81 1.0822496672924196 1104 82-83 1.3643805070419326 1104 84-85 1.322767330154992 1104 86-87 1.3980183421070862 1104 88-89 1.254024121248296 1104 90-91 1.4895493136935656 1104 92-93 1.6424944644259227 1104 94-95 1.6840567379735454 1104 96-97 1.7423099271930695 1104 98-99 1.8273156468898293 1104 100-101 1.5377206653990285 1105 1 0.19381386110638488 1105 2 0.12567207396996594 1105 3 0.2390133585616283 1105 4 0.11086317687208691 1105 5 0.26847409125376487 1105 6 0.2385246703968349 1105 7 0.15507385932775009 1105 8 0.22760964485825497 1105 9 0.30509865644728507 1105 10-11 0.2967173615384908 1105 12-13 0.283118523472325 1105 14-15 0.43512457460648335 1105 16-17 0.2434738211282763 1105 18-19 0.29252919371722186 1105 20-21 0.3948937781420412 1105 22-23 0.45340057465548256 1105 24-25 0.31823712120839787 1105 26-27 0.43645044810818234 1105 28-29 0.23533869029983379 1105 30-31 0.2607118764565328 1105 32-33 0.34614723335131004 1105 34-35 0.44429983431198394 1105 36-37 0.418506884775816 1105 38-39 0.498168827921468 1105 40-41 0.45208001149412524 1105 42-43 0.4495042114618073 1105 44-45 0.4045073693367911 1105 46-47 0.615754799500003 1105 48-49 0.6875680037672183 1105 50-51 0.6329234895498459 1105 52-53 0.6236017847051087 1105 54-55 0.777156777388889 1105 56-57 0.8141312319511442 1105 58-59 0.7831888896423678 1105 60-61 1.0362318787615195 1105 62-63 0.9188276558724482 1105 64-65 0.9757247810026186 1105 66-67 0.9814624061398405 1105 68-69 0.8584018552409844 1105 70-71 0.9761959036811163 1105 72-73 1.0116888000929798 1105 74-75 1.0243936868901464 1105 76-77 1.173932666382342 1105 78-79 1.0930777062492751 1105 80-81 1.1085317196127598 1105 82-83 1.1807053848742228 1105 84-85 1.2854219530912623 1105 86-87 1.0452059337227482 1105 88-89 1.2255582755659127 1105 90-91 1.3517818364628411 1105 92-93 1.6817284400879444 1105 94-95 1.5437151138111496 1105 96-97 1.7647653807239863 1105 98-99 1.5697983036912966 1105 100-101 1.2868433821950838 1106 1 0.07551771554070896 1106 2 -0.3281935339718203 1106 3 -0.03273998867477701 1106 4 0.12973857964938418 1106 5 0.046415936157202964 1106 6 -0.041943677063038365 1106 7 0.07070419051377996 1106 8 0.01826748844278825 1106 9 0.2630183902368941 1106 10-11 -0.20871069765048134 1106 12-13 -0.05337771671069902 1106 14-15 -0.1304439011180918 1106 16-17 -0.042203540447459886 1106 18-19 0.26664413777745466 1106 20-21 0.17584364528139673 1106 22-23 0.26188054942419114 1106 24-25 0.10398595177333902 1106 26-27 0.11182564976036957 1106 28-29 0.2761181787420952 1106 30-31 0.32660754944142667 1106 32-33 0.26921160435252034 1106 34-35 0.40975332718490165 1106 36-37 0.1997533798438198 1106 38-39 0.34803229896154875 1106 40-41 0.37473686168078757 1106 42-43 0.40642907162292374 1106 44-45 0.3242181792427772 1106 46-47 0.4387191560809285 1106 48-49 0.6149342176142412 1106 50-51 0.5457319551886499 1106 52-53 0.5271279254893173 1106 54-55 0.5230887372245903 1106 56-57 0.5206804055672407 1106 58-59 0.30783894458283356 1106 60-61 0.32874601865858466 1106 62-63 0.4554796520708848 1106 64-65 0.7739284005801395 1106 66-67 0.897954792478842 1106 68-69 0.7459514091817283 1106 70-71 0.9925148670392332 1106 72-73 0.8326425379421991 1106 74-75 0.8215672254020241 1106 76-77 0.8971625755851065 1106 78-79 0.887678782795728 1106 80-81 0.9607245737257877 1106 82-83 0.9774931173381773 1106 84-85 0.6917880901987203 1106 86-87 0.674078224752904 1106 88-89 0.9610924461756731 1106 90-91 1.023222659718133 1106 92-93 1.0911439207348153 1106 94-95 1.1274506271102815 1106 96-97 1.1380780590275208 1106 98-99 1.3799092050101223 1106 100-101 0.8840981297177031 1107 1 0.07348086145368526 1107 2 0.18154573839620625 1107 3 0.020512878470103857 1107 4 0.1488202342174887 1107 5 -0.04134919420661021 1107 6 0.12255784778555068 1107 7 0.10212895749114637 1107 8 -0.07332479711195816 1107 9 -0.05733898272818294 1107 10-11 0.1111376765602472 1107 12-13 0.20713944320511501 1107 14-15 0.27112347141744664 1107 16-17 0.31180616703933595 1107 18-19 0.2699820528154717 1107 20-21 0.2850958251705791 1107 22-23 0.3575293617604345 1107 24-25 0.22594677819832043 1107 26-27 0.32598956024531134 1107 28-29 0.2377959417247837 1107 30-31 0.2574004707076938 1107 32-33 0.23366158502345513 1107 34-35 0.3406527730849973 1107 36-37 0.36661777570310505 1107 38-39 0.4752811284791889 1107 40-41 0.3739701420381678 1107 42-43 0.1826942664169664 1107 44-45 0.26979654786955365 1107 46-47 0.3719230562570388 1107 48-49 0.4327813891274914 1107 50-51 0.3132092972272247 1107 52-53 0.4716938805599824 1107 54-55 0.6594299596806721 1107 56-57 0.5285666647485456 1107 58-59 0.7180335238534852 1107 60-61 0.5916724393449826 1107 62-63 0.3727027094996913 1107 64-65 0.15263850753431285 1107 66-67 0.5733398704394119 1107 68-69 0.5905165051827623 1107 70-71 0.4954361333078623 1107 72-73 0.7389665790057265 1107 74-75 0.5849197446237042 1107 76-77 0.7327173197971248 1107 78-79 0.8572187510101514 1107 80-81 1.010164030793785 1107 82-83 1.0205879156715838 1107 84-85 0.9768127884505127 1107 86-87 1.0892773141390961 1107 88-89 1.0873043786527994 1107 90-91 1.3328176083887229 1107 92-93 1.3581157002975495 1107 94-95 1.547320907596518 1107 96-97 1.3516045398487897 1107 98-99 1.310613642104748 1107 100-101 1.30160854593694 1108 1 0.07849158610355644 1108 2 0.0505965017389336 1108 3 0.12699140814163457 1108 4 0.21364842953228447 1108 5 -0.012921722279962466 1108 6 0.12123640269155089 1108 7 0.12533625866454656 1108 8 -0.038433095046933374 1108 9 0.21540694821348438 1108 10-11 0.10518107984913883 1108 12-13 0.14329833625301092 1108 14-15 0.17613125204578495 1108 16-17 0.1953953498630696 1108 18-19 0.18686307228806953 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2.2147073342291783 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5596.0 3 985.0 4 958.0 5 1085.0 6 1253.0 7 1449.0 8 1789.0 9 2371.0 10 2950.0 11 3815.0 12 5221.0 13 6460.0 14 7372.0 15 9404.0 16 11633.0 17 15124.0 18 13219.0 19 10308.0 20 10543.0 21 11333.0 22 12833.0 23 14156.0 24 16363.0 25 18897.0 26 21945.0 27 25670.0 28 30412.0 29 36197.0 30 43750.0 31 53688.0 32 66344.0 33 84050.0 34 109661.0 35 146040.0 36 196144.0 37 237698.0 38 156692.0 39 14572.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.26608787566036 19.59619960953331 14.839585999896007 22.298126514910315 2 14.744916994426424 20.601587486934704 40.94101463598019 23.712480882658685 3 19.40827205843087 25.13964412020822 29.80847484056184 25.643608980799065 4 12.003226796365084 16.7883835330857 38.71237154580776 32.496018124741454 5 12.967546245532091 38.33921934408954 34.55854919328425 14.134685217094114 6 30.69615117417944 37.189158485752685 17.96474614491037 14.149944195157508 7 27.881611400383665 33.21927729710684 21.01034618984742 17.88876511266207 8 25.82381091694944 35.207564119264575 19.936676197980844 19.031948765805147 9 25.88550368795245 17.38708871374783 20.954304414651006 35.773103183648715 10-11 24.32255122625125 27.3694822075477 27.70469587466846 20.60327069153259 12-13 25.527128562880797 24.68545987622352 28.211954144255447 21.575457416640237 14-15 22.33942746830974 26.091968668496516 26.949463571859734 24.61914029133401 16-17 21.224065193886897 29.612781395626932 27.36926807775833 21.793885332727843 18-19 21.11438974674319 28.332429454769066 29.797815887765328 20.75536491072242 20-21 22.128045887920077 27.08669857440114 29.700883672713335 21.084371864965444 22-23 21.5747734474948 27.045057836946253 29.805003816651276 21.575164898907673 24-25 21.53421241150766 27.264555128151745 29.77758987668635 21.423642583654242 26-27 21.377002160073165 27.320334935891733 30.044696077349837 21.257966826685266 28-29 20.984376017672943 27.886062627615075 29.91292011763454 21.216641237077443 30-31 21.393447086876343 27.341179117158887 29.73485151848771 21.53052227747706 32-33 21.226210106671687 27.44264497095545 29.74353736000476 21.58760756236811 34-35 21.821981039034164 27.417021305371776 29.550975257922023 21.210022397672038 36-37 21.624193575939096 27.140546973311846 29.892175480153284 21.34308397059577 38-39 21.173283477141972 27.698155586616828 29.78432974547152 21.344231190769676 40-41 21.439932704645557 27.621981725781392 29.607892116779073 21.330193452793974 42-43 21.27396228241463 27.596584897663302 29.259972192061962 21.869480627860106 44-45 21.51331430907901 27.51695048249224 29.252477761443824 21.717257446984924 46-47 21.71141699159944 27.91948358110792 28.877163311993826 21.491936115298817 48-49 21.639299686231187 27.779537546908724 28.887409101003946 21.693753665856143 50-51 21.420425301078737 28.083062634770865 28.977524722378245 21.518987341772153 52-53 21.703291031094814 28.303508178630548 28.293291565064166 21.699909225210472 54-55 21.431261113381044 27.966356263142085 28.590876670477307 22.011505952999563 56-57 21.743108133738374 28.149658764949383 28.392094178667747 21.715138922644492 58-59 21.974731357446792 27.876239228412974 28.52056862095577 21.628460793184463 60-61 21.723740518776 28.123346556728297 28.893266244974512 21.259646679521197 62-63 21.47642409269168 28.127296270847246 28.900142086345653 21.496137550115417 64-65 21.35967652638755 28.165495578710065 28.71027563857147 21.764552256330923 66-67 21.520492140139957 28.096771335519723 28.28891350364483 22.093823020695492 68-69 21.47872263960677 28.023653925941105 28.515228308991237 21.982395125460886 70-71 21.704814269732328 28.004719760652907 28.448371568708385 21.842094400906376 72-73 21.692794475156994 27.729008557130086 28.67958728785093 21.898609679861995 74-75 21.36378986219194 28.495277760084374 28.293620447987394 21.84731192973629 76-77 21.48675507883606 28.055531336022593 28.294041342446203 22.163672242695142 78-79 21.601760611374008 27.986966237273275 28.332241373726703 22.079031777626014 80-81 21.691562684157933 28.199081309909158 28.14321117345791 21.966144832475 82-83 21.783952124104978 28.031367849164457 28.449157481889614 21.73552254484095 84-85 21.580609512505276 28.134288583043894 28.28977283643839 21.995329068012442 86-87 21.498542979438042 28.1678188811363 28.304519076473124 22.02911906295254 88-89 21.627693889787373 27.910343931864805 28.13565835615122 22.326303822196603 90-91 21.60600050243782 27.74759118072176 28.35941241818483 22.286995898655587 92-93 21.913928515382718 27.646262773561585 28.48342768608202 21.956381024973677 94-95 21.518246398615386 27.622239270571054 28.553026928009313 22.306487402804247 96-97 21.31889936803658 27.673158297602495 28.73054672540177 22.277395608959154 98-99 21.401502952418603 27.87212698335877 28.18308020194522 22.5432898622774 100-101 21.711896225596035 26.957298665956923 28.83361253399867 22.49719257444837 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2000.0 1 1094.5 2 203.5 3 237.5 4 236.0 5 203.5 6 297.5 7 650.0 8 1100.5 9 1361.5 10 1387.5 11 1359.0 12 1409.5 13 1521.0 14 1650.5 15 1768.5 16 1857.5 17 1916.5 18 2085.0 19 2279.5 20 2461.0 21 2914.0 22 3555.5 23 4328.0 24 5220.0 25 6567.5 26 8578.0 27 10671.0 28 12589.5 29 15207.5 30 18352.0 31 21459.0 32 25095.5 33 28897.5 34 33250.5 35 36956.5 36 40732.5 37 44438.0 38 46094.5 39 47345.5 40 48274.5 41 49487.0 42 50526.0 43 51671.0 44 53204.0 45 54260.5 46 54591.5 47 56321.5 48 61378.0 49 60257.5 50 52601.5 51 46980.5 52 43236.5 53 41131.0 54 38995.5 55 35031.0 56 30501.0 57 26553.0 58 23390.5 59 19896.0 60 16212.5 61 13175.5 62 10566.0 63 8119.5 64 5993.0 65 4492.5 66 3379.0 67 2598.5 68 1941.0 69 1365.0 70 987.5 71 689.0 72 493.0 73 370.5 74 264.0 75 220.5 76 173.5 77 106.5 78 66.0 79 43.0 80 22.0 81 16.0 82 12.5 83 9.5 84 10.0 85 7.0 86 5.0 87 5.0 88 5.5 89 3.5 90 1.5 91 1.0 92 1.5 93 2.5 94 2.0 95 1.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2856568580728603 2 0.24851152322151615 3 0.25000301850235407 4 0.24815640529750713 5 0.2502160892567595 6 0.21924980628317245 7 0.22145153741202842 8 0.22301405627766813 9 0.23253121664111 10-11 0.23839066238725892 12-13 0.2324957048487091 14-15 0.2226589383536591 16-17 0.21775831100233453 18-19 0.20788603271488362 20-21 0.21268012468900546 22-23 0.20991020488173506 24-25 0.21225398318019464 26-27 0.20866729214770346 28-29 0.22184216712843835 30-31 0.23292184635751995 32-33 0.22432799259650152 34-35 0.2236887803332853 36-37 0.2141361081774425 38-39 0.20419280630518977 40-41 0.20128083932831575 42-43 0.21225398318019464 44-45 0.20841870960089715 46-47 0.21853957043515437 48-49 0.22209074967524467 50-51 0.23324145248912806 52-53 0.2420483770045519 54-55 0.22336917420167718 56-57 0.2035891058343744 58-59 0.19343273320771628 60-61 0.19684186527820294 62-63 0.20252375206234735 64-65 0.19439155160254065 66-67 0.19698391244780655 68-69 0.1991501317842616 70-71 0.20078367423470314 72-73 0.20163595725232478 74-75 0.20429934168239247 76-77 0.21381650204583438 78-79 0.2142426435546452 80-81 0.20873831573250531 82-83 0.20241721668514462 84-85 0.19147958462566664 86-87 0.19304210349130635 88-89 0.20138737470551843 90-91 0.20128083932831575 92-93 0.2047965067760051 94-95 0.2009257214043067 96-97 0.20721130865926649 98-99 0.20941303978812245 100-101 0.19751658933382008 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1407983.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.649504409006084 #Duplication Level Percentage of deduplicated Percentage of total 1 80.42992991485337 50.388952488166574 2 11.822298212052225 14.81322247921101 3 3.4626539993832575 6.508006710036717 4 1.4728866531152935 3.691024754732512 5 0.7416852294337399 2.3233106025751886 6 0.44847720805890195 1.685812489417495 7 0.3060531031647087 1.3421852665275187 8 0.20298675631698365 1.0173615747880567 9 0.15576663285605993 0.8782832116702582 >10 0.8748857715173263 9.86646468427315 >50 0.05563837210363742 2.3988820019885337 >100 0.02458555646942411 2.6015440249311705 >500 6.797654763674128E-4 0.31159466536083635 >1k 0.0013595309527348256 1.792059706385056 >5k 1.1329424606123545E-4 0.3812953399358571 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5372 0.3815386975552972 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4322 0.3069639335134018 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3919 0.27834142883827434 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2696 0.19147958462566664 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2577 0.18302777803425185 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2215 0.15731724033599837 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1904 0.135228905462637 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1635 0.11612356115095139 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.102358480180514E-5 0.0 10-11 0.0 0.0 0.0 7.102358480180514E-5 0.0 12-13 0.0 0.0 0.0 1.4204716960361028E-4 0.0 14-15 0.0 0.0 0.0 1.4204716960361028E-4 0.0 16-17 0.0 0.0 0.0 1.4204716960361028E-4 0.0 18-19 0.0 0.0 0.0 1.4204716960361028E-4 0.0 20-21 0.0 0.0 0.0 1.4204716960361028E-4 0.0 22-23 0.0 0.0 0.0 2.130707544054154E-4 0.0 24-25 0.0 0.0 0.0 2.8409433920722056E-4 0.0 26-27 0.0 0.0 0.0 7.102358480180513E-4 0.0 28-29 0.0 0.0 0.0 0.0018111014124460312 0.0 30-31 0.0 0.0 0.0 0.0030540141464776207 0.0 32-33 0.0 0.0 0.0 0.004794091974121847 0.0 34-35 0.0 0.0 0.0 0.00678275234857239 0.0 36-37 0.0 0.0 0.0 0.010689049512671673 0.0 38-39 7.102358480180514E-5 0.0 0.0 0.014204716960361026 0.0 40-41 7.102358480180514E-5 0.0 0.0 0.018182037709262112 0.0 42-43 7.102358480180514E-5 0.0 0.0 0.024609672133825478 0.0 44-45 1.065353772027077E-4 0.0 0.0 0.03437541504407369 0.0 46-47 1.4204716960361028E-4 0.0 0.0 0.05607312020102516 0.0 48-49 1.4204716960361028E-4 0.0 0.0 0.10284215079301384 0.0 50-51 1.4204716960361028E-4 0.0 0.0 0.1393482733811417 0.0 52-53 1.4204716960361028E-4 0.0 0.0 0.17617400210087764 0.0 54-55 1.4204716960361028E-4 0.0 0.0 0.22986783221104232 0.0 56-57 1.4204716960361028E-4 0.0 0.0 0.27095497601888663 0.0 58-59 1.4204716960361028E-4 0.0 0.0 0.3124327495431408 0.0 60-61 1.4204716960361028E-4 0.0 0.0 0.38512538858778833 0.0 62-63 1.4204716960361028E-4 0.0 0.0 0.4404172493559937 0.0 64-65 1.4204716960361028E-4 0.0 0.0 0.4985500535162712 0.0 66-67 1.4204716960361028E-4 0.0 0.0 0.6155614094772451 0.0 68-69 1.4204716960361028E-4 0.0 0.0 0.7518556687119091 0.0 70-71 1.4204716960361028E-4 0.0 0.0 0.8767861543782844 0.0 72-73 1.4204716960361028E-4 0.0 0.0 1.021638755581566 0.0 74-75 1.4204716960361028E-4 0.0 0.0 1.15793301481623 0.0 76-77 1.4204716960361028E-4 0.0 0.0 1.2697951608790732 0.0 78-79 1.4204716960361028E-4 0.0 0.0 1.4157131158543819 0.0 80-81 2.8409433920722056E-4 0.0 0.0 1.6472500023082666 0.0 82-83 2.8409433920722056E-4 0.0 0.0 1.8469683227709426 0.0 84-85 3.196061316081231E-4 0.0 0.0 2.0517648295469475 0.0 86-87 3.5511792400902566E-4 0.0 0.0 2.420377234668316 0.0 88-89 3.5511792400902566E-4 0.0 0.0 2.8923644674687123 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 6240 0.0 27.253319 2 GTACATG 6325 0.0 27.12687 1 ACATGGG 6430 0.0 24.96958 3 GTATCAA 10375 0.0 22.58457 1 CATGGGA 4255 0.0 22.554575 4 GAGTACT 4880 0.0 20.44077 12-13 GTATTAG 595 0.0 19.969908 1 AGAGTAC 8420 0.0 19.689482 10-11 AGTACTT 5125 0.0 19.602627 12-13 GTGTAGC 860 0.0 19.342945 1 TCAACGC 11915 0.0 19.298971 4 ATCAACG 11950 0.0 19.19927 3 CAACGCA 11920 0.0 19.173353 5 TATCAAC 12065 0.0 19.056318 2 AACGCAG 12145 0.0 18.813454 6 CATGGGG 2280 0.0 18.753853 4 GTACTTT 5250 0.0 18.727417 14-15 ATGGGAT 1930 0.0 18.710506 5 GTAGCCC 700 0.0 18.32193 3 GGTATCA 7900 0.0 18.048754 1 >>END_MODULE