FastQCFastQC Report
Thu 26 May 2016
SRR937207_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937207_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1407983
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66530.4725199096864096No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51740.36747602776453975No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT47670.33856942875020507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36140.25667923547372373No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22070.15674905165758393No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20520.14574039601330413No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC15720.11164907530843766No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15570.1105837215364106No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAG650.005845901629.2293473
GTACATG64850.025.0631391
TACATGG64600.024.6311552
ACATGGG64150.023.545233
GAGTACT60350.022.47310312-13
GGACCGA1703.15115E-622.3518546
GTATCAA126050.021.7169571
GTACTTT63250.021.40212214-15
GTATAGG4450.021.3594131
TACGCTG1150.003724736420.651175
AGAGTAC95450.020.45502910-11
AGTACTT64350.020.37486512-13
CCTATAC3052.0918378E-1020.2449193
TAGGACA6950.019.819184
CATGGGG24300.019.741844
CATGGGA42350.019.290684
TAGGGCG1250.00603349718.9990775
TCAACGC143100.018.9526084
CAACGCA142900.018.9458965
ATCAACG144050.018.8276183
Produced by FastQC (version 0.11.5)