FastQCFastQC Report
Thu 26 May 2016
SRR937206_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937206_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375981
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54720.39767990982433626No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41660.30276580853950746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38800.2819806378140396No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26570.19309859656492348No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT25030.18190658155890232No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18310.13306869789626455No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18300.13299602247414752No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC16120.11715278045263705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG64650.026.1110081
TACATGG63900.025.6639652
ACATGGG64350.024.3764743
GTATCAA108200.022.36941
GAGTACT47050.021.90771912-13
GTGTAAG6200.021.474851
CCATGCG1353.7957955E-421.1116629
CATGGGA40100.021.1014754
AGTACTT50600.020.32377612-13
AGAGTAC83350.020.28847110-11
TCAACGC121250.019.603134
GTACTTT52150.019.53683314-15
ATCAACG121900.019.5361753
CAACGCA122100.019.4284385
AACGCAG124550.019.183566
TATCAAC124400.019.1435662
CATGGGG25400.018.9027444
GAGTACA51300.018.6312731
ATGGGAG17500.018.4724245
GTATAGG4650.018.4070151
Produced by FastQC (version 0.11.5)