##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937206_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1375981 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.756012619360295 33.0 31.0 34.0 30.0 34.0 2 31.866928395086852 33.0 31.0 34.0 30.0 34.0 3 31.907386802579396 33.0 31.0 34.0 30.0 34.0 4 35.52110385245145 37.0 35.0 37.0 33.0 37.0 5 35.536638950683184 37.0 35.0 37.0 33.0 37.0 6 35.58017080177706 37.0 35.0 37.0 33.0 37.0 7 35.5246584073472 37.0 35.0 37.0 33.0 37.0 8 35.51417207068993 37.0 35.0 37.0 33.0 37.0 9 37.13638560416169 39.0 37.0 39.0 34.0 39.0 10-11 37.02140509207612 39.0 37.0 39.0 33.0 39.0 12-13 36.89791247117511 39.0 37.0 39.0 33.0 39.0 14-15 38.07610933581205 40.0 38.0 41.0 33.0 41.0 16-17 38.04616270137451 40.0 38.0 41.0 33.0 41.0 18-19 38.02469038453293 40.0 38.0 41.0 33.0 41.0 20-21 37.967631820497516 40.0 38.0 41.0 33.0 41.0 22-23 37.81902221033575 40.0 38.0 41.0 33.0 41.0 24-25 37.662661403028096 40.0 37.0 41.0 32.0 41.0 26-27 37.50770286799018 40.0 37.0 41.0 32.0 41.0 28-29 37.58230818594152 40.0 37.0 41.0 32.0 41.0 30-31 37.45152876384194 40.0 37.0 41.0 32.0 41.0 32-33 37.273834449748946 40.0 36.5 41.0 32.0 41.0 34-35 37.153798998678035 39.5 36.5 41.0 31.0 41.0 36-37 37.1654695813387 40.0 37.0 41.0 31.0 41.0 38-39 37.09351400927774 40.0 37.0 41.0 31.0 41.0 40-41 36.94036436549633 40.0 36.0 41.0 31.0 41.0 42-43 36.71476459340644 39.0 36.0 41.0 30.0 41.0 44-45 36.50491867256888 39.0 36.0 41.0 30.0 41.0 46-47 36.1864277195688 39.0 36.0 41.0 29.5 41.0 48-49 35.89074849143992 39.0 35.0 41.0 28.5 41.0 50-51 35.0251322510994 38.0 34.0 40.0 27.0 40.5 52-53 35.136449921910256 38.5 35.0 40.0 27.5 40.5 54-55 35.48162220263216 39.0 35.0 40.0 27.5 41.0 56-57 35.29832970077348 39.0 35.0 40.0 26.5 41.0 58-59 35.07898837265922 38.5 35.0 40.0 26.0 41.0 60-61 34.79072421784894 38.0 34.0 40.0 26.0 41.0 62-63 34.48805942814617 38.0 34.0 40.0 26.0 41.0 64-65 34.1450437905756 37.5 34.0 40.0 25.0 41.0 66-67 33.76342878281023 37.0 33.5 39.5 23.5 41.0 68-69 33.293498602088256 36.0 33.0 39.0 22.0 41.0 70-71 32.85472546495919 36.0 32.0 39.0 21.5 40.0 72-73 32.456391112958684 35.0 32.0 38.5 20.5 40.0 74-75 32.0072708126057 35.0 31.5 37.5 19.5 39.5 76-77 31.566113921631185 35.0 31.0 37.0 18.0 39.0 78-79 31.085000446953845 35.0 31.0 36.5 15.0 39.0 80-81 30.585536064814846 34.5 30.5 36.0 9.5 38.0 82-83 30.099967223384624 34.0 30.0 35.5 7.0 37.0 84-85 29.652422526183138 34.0 29.5 35.0 4.0 37.0 86-87 29.24089867519973 34.0 29.0 35.0 2.0 36.0 88-89 28.821701026394987 34.0 29.0 35.0 2.0 36.0 90-91 28.386885792754406 34.0 29.0 35.0 2.0 35.5 92-93 27.94676961382461 34.0 27.5 35.0 2.0 35.0 94-95 27.42536343161715 33.0 26.5 35.0 2.0 35.0 96-97 26.794109439011148 33.0 25.5 35.0 2.0 35.0 98-99 26.045332384676822 32.5 24.5 35.0 2.0 35.0 100-101 24.09808274968913 30.5 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.058634520231578335 1101 2 0.07863745314039505 1101 3 0.17038962260565071 1101 4 0.04796622782203741 1101 5 0.08057266532284046 1101 6 0.07101201158348402 1101 7 0.023528953940832764 1101 8 0.06098914013085732 1101 9 0.1489636175519209 1101 10-11 0.1244003937774707 1101 12-13 0.04466659702091391 1101 14-15 0.14155072550241243 1101 16-17 0.27542725811726854 1101 18-19 0.3028383014066165 1101 20-21 0.23484431668254757 1101 22-23 0.12872506554553098 1101 24-25 -0.034644120735706 1101 26-27 0.08926337392114903 1101 28-29 0.007739154662772307 1101 30-31 0.1649777081746251 1101 32-33 0.09569058061560298 1101 34-35 -0.08571052403548407 1101 36-37 0.21642640739076313 1101 38-39 0.18397447678474776 1101 40-41 0.2223027129427848 1101 42-43 0.35363119103625706 1101 44-45 0.4919836037193406 1101 46-47 0.4623475237974972 1101 48-49 0.39302816605262336 1101 50-51 0.34862070954208235 1101 52-53 0.36251175916761724 1101 54-55 0.5270986537334252 1101 56-57 0.2921788183902052 1101 58-59 0.40241204528236096 1101 60-61 0.22107158573411567 1101 62-63 0.41952739013756 1101 64-65 0.3715434685468111 1101 66-67 0.36757909991994353 1101 68-69 0.4427347741217318 1101 70-71 0.3491195683096606 1101 72-73 0.41115061252825313 1101 74-75 0.3849985050910938 1101 76-77 0.4340140272357189 1101 78-79 0.39572265399885254 1101 80-81 0.5059137528466024 1101 82-83 0.4737221682329036 1101 84-85 0.45123623742627217 1101 86-87 0.6003071633390249 1101 88-89 0.28968533725939594 1101 90-91 0.5651352351224155 1101 92-93 0.8410396533533664 1101 94-95 0.7547978643041162 1101 96-97 0.7575320511341168 1101 98-99 0.7515562327438694 1101 100-101 1.2000605580772614 1102 1 0.15775350000120625 1102 2 0.19956995715194736 1102 3 0.29779391339778627 1102 4 0.19075340016748044 1102 5 0.14280864994568532 1102 6 0.0983574484353511 1102 7 0.2503767261671399 1102 8 0.16883388737196725 1102 9 0.20168708810975033 1102 10-11 0.2824956727612502 1102 12-13 0.2982365625493131 1102 14-15 0.30259639365563373 1102 16-17 0.5100652169409798 1102 18-19 0.5051405816837473 1102 20-21 0.406202483158701 1102 22-23 0.4268238507214974 1102 24-25 0.4541292665500549 1102 26-27 0.43663775474728794 1102 28-29 0.42791782735436357 1102 30-31 0.37373764313117874 1102 32-33 0.24449230365861752 1102 34-35 0.2578759170184526 1102 36-37 0.2789836737057385 1102 38-39 0.3219878611367548 1102 40-41 0.4424745879148304 1102 42-43 0.4589040747541162 1102 44-45 0.4257108470115156 1102 46-47 0.4490971584200807 1102 48-49 0.3432507913676872 1102 50-51 0.3213981683040714 1102 52-53 0.5302145414026711 1102 54-55 0.4660558477779091 1102 56-57 0.5411435078149225 1102 58-59 0.6698944382943068 1102 60-61 0.47892894453384827 1102 62-63 0.3981228023924146 1102 64-65 0.4437034081544411 1102 66-67 0.4735305033806938 1102 68-69 0.5153685863365212 1102 70-71 0.4794026933286659 1102 72-73 0.5250777087750151 1102 74-75 0.6925749212975667 1102 76-77 0.6681346438246578 1102 78-79 0.478543922274433 1102 80-81 0.4828517737442226 1102 82-83 0.5938158694301947 1102 84-85 0.7213448757178256 1102 86-87 0.6080714112697443 1102 88-89 0.7230124095352544 1102 90-91 0.6315570049981396 1102 92-93 0.6827173663142929 1102 94-95 0.5962889977522963 1102 96-97 0.7009032906982746 1102 98-99 0.8445440272595413 1102 100-101 1.3712763302177002 1103 1 0.11545561380920333 1103 2 -0.005020067314820409 1103 3 0.08662122774444114 1103 4 -0.029624719783313935 1103 5 -0.0021040430793277665 1103 6 0.08784081816327216 1103 7 0.17904347236952844 1103 8 0.19889724786815322 1103 9 0.10370720658240629 1103 10-11 0.24626397376000853 1103 12-13 0.16316241327346148 1103 14-15 0.16323093627503482 1103 16-17 0.04896962122538895 1103 18-19 0.0625742052462428 1103 20-21 0.01610357552979025 1103 22-23 -0.050028394644435537 1103 24-25 0.04165026379082093 1103 26-27 0.12178758366290054 1103 28-29 -0.017170504945383414 1103 30-31 0.025422244087359047 1103 32-33 0.0012585894558867494 1103 34-35 -0.054561119846361805 1103 36-37 0.06857464535912783 1103 38-39 0.006997231995207187 1103 40-41 0.16169816931704162 1103 42-43 0.18249024148411763 1103 44-45 0.308813151933947 1103 46-47 0.31731453031832046 1103 48-49 0.12231217325389565 1103 50-51 0.016295443341533655 1103 52-53 -0.07412425943999779 1103 54-55 0.09937207965987227 1103 56-57 0.06196331785159259 1103 58-59 0.007147383625436987 1103 60-61 0.08047783289504196 1103 62-63 -0.12367487778173114 1103 64-65 0.10030499401548099 1103 66-67 0.06691014530984063 1103 68-69 -0.04567273196430932 1103 70-71 0.11299564854713395 1103 72-73 0.22461276783028694 1103 74-75 0.19523714463617026 1103 76-77 0.25966031563154246 1103 78-79 0.15827992211281838 1103 80-81 0.14029687027149507 1103 82-83 0.21833984621135727 1103 84-85 0.3120504782981399 1103 86-87 0.3281859579599491 1103 88-89 0.33321633481385504 1103 90-91 0.44822855688624585 1103 92-93 0.37281295783710533 1103 94-95 0.34745716282881745 1103 96-97 0.3132994670373641 1103 98-99 0.43466549965638634 1103 100-101 0.7699485155964219 1104 1 0.0811861694186291 1104 2 0.0518553621179052 1104 3 0.1146775624736307 1104 4 0.0892502434760658 1104 5 0.06558463069553255 1104 6 0.04170942952565326 1104 7 0.08945681907207614 1104 8 0.06860522030688543 1104 9 0.14406504215401128 1104 10-11 0.202563454771429 1104 12-13 0.14411356659621077 1104 14-15 0.35526887807028373 1104 16-17 0.35334298120588414 1104 18-19 0.3067186155578696 1104 20-21 0.3612537947567418 1104 22-23 0.2759767773701043 1104 24-25 0.2177358373988696 1104 26-27 0.24239016404262514 1104 28-29 0.29096247081093907 1104 30-31 0.29015585035644165 1104 32-33 0.25028051899526815 1104 34-35 0.325927206237715 1104 36-37 0.5439151781427896 1104 38-39 0.4575157452748684 1104 40-41 0.524226273752042 1104 42-43 0.6343854110385081 1104 44-45 0.6577439000965342 1104 46-47 0.7136075016334544 1104 48-49 0.6417264298690171 1104 50-51 0.5781588515097553 1104 52-53 0.5526503647600265 1104 54-55 0.581285998812632 1104 56-57 0.5665258088446805 1104 58-59 0.46316187530447195 1104 60-61 0.5135133248086845 1104 62-63 0.23425898568119408 1104 64-65 0.4672279039257674 1104 66-67 0.5269728543701504 1104 68-69 0.45249926774823734 1104 70-71 0.5546766407282675 1104 72-73 0.7479343631773787 1104 74-75 0.6693053968245444 1104 76-77 0.6224030651340939 1104 78-79 0.6250295189641335 1104 80-81 0.6511144873652306 1104 82-83 0.6556108233464286 1104 84-85 0.7542187471029678 1104 86-87 0.6770284906120416 1104 88-89 0.6263507725901682 1104 90-91 0.57665893298471 1104 92-93 0.5952403825183623 1104 94-95 0.7991966244024091 1104 96-97 0.9486733924336583 1104 98-99 0.9888737407341672 1104 100-101 1.196931358820347 1105 1 -0.10285190453643622 1105 2 -0.12521078796303087 1105 3 -0.044437013111743084 1105 4 -0.13687722581584438 1105 5 -0.05553643086169302 1105 6 -0.062347112810023475 1105 7 0.02967259939972422 1105 8 0.011784095829341368 1105 9 0.006559353429977932 1105 10-11 -0.09390749774962615 1105 12-13 -0.12381404087798131 1105 14-15 0.0729158785562376 1105 16-17 -0.05548977526603949 1105 18-19 -0.1509032829167154 1105 20-21 -0.158672992880021 1105 22-23 0.040014564822847376 1105 24-25 0.014316983121666738 1105 26-27 0.0649289713334511 1105 28-29 -0.10733195881251589 1105 30-31 -0.3894193989281334 1105 32-33 -0.24186711344869138 1105 34-35 -0.24652262004270398 1105 36-37 -0.27214922757976723 1105 38-39 -0.1605645348803293 1105 40-41 -0.28358355922812706 1105 42-43 -0.11230741549425005 1105 44-45 -0.3091254118681377 1105 46-47 -0.16952324149847442 1105 48-49 -0.05637890822026037 1105 50-51 -0.1983120348953733 1105 52-53 -0.11082565775024023 1105 54-55 -0.11210913086823382 1105 56-57 -0.13218889765021657 1105 58-59 -0.11828742976210549 1105 60-61 -0.13557256748627822 1105 62-63 -0.18525094107338447 1105 64-65 -0.2438758242408241 1105 66-67 -0.185649887082441 1105 68-69 -0.01987033788131498 1105 70-71 -0.013896588714906954 1105 72-73 0.01372918123187361 1105 74-75 -0.14851052983967605 1105 76-77 -0.12798123365697833 1105 78-79 -0.25677504898484926 1105 80-81 -0.07122784544688798 1105 82-83 -0.15774668637536848 1105 84-85 -0.0648847871235958 1105 86-87 -0.06891281828484708 1105 88-89 -0.021741177025088376 1105 90-91 0.07926390027385466 1105 92-93 0.22342758313720523 1105 94-95 0.09009400477942364 1105 96-97 0.06958453594156921 1105 98-99 0.09527960213095454 1105 100-101 0.4624093714198345 1106 1 0.021548891995507802 1106 2 -0.115577985483732 1106 3 -0.16111566481274764 1106 4 -0.05342355964980783 1106 5 -0.1029797972320381 1106 6 -0.10498372772681819 1106 7 -0.08782319618846657 1106 8 -0.15291961134828824 1106 9 -0.21424705217523865 1106 10-11 -0.47978584637015587 1106 12-13 -0.5119726339546062 1106 14-15 -0.40737481932015385 1106 16-17 -0.4040000991861987 1106 18-19 -0.17773374119278174 1106 20-21 -0.10415768426635452 1106 22-23 -0.0686281310031589 1106 24-25 -0.11395183367174155 1106 26-27 -0.12487904038216158 1106 28-29 0.09756619541499134 1106 30-31 -0.03754438758962664 1106 32-33 0.04023897938765231 1106 34-35 0.034235204679355036 1106 36-37 -0.004998373245605592 1106 38-39 0.0018834382979022735 1106 40-41 -0.08777029262499525 1106 42-43 -0.12650109287719857 1106 44-45 -0.06441127520168521 1106 46-47 0.08642942205478477 1106 48-49 0.001404396955003051 1106 50-51 -0.11826030432203538 1106 52-53 -0.24578158331719635 1106 54-55 -0.18081850285629741 1106 56-57 -0.08230336341296862 1106 58-59 -0.10451776398051038 1106 60-61 -0.17917126607751754 1106 62-63 -0.21251235879679342 1106 64-65 -0.13819985865427498 1106 66-67 0.09116310508077419 1106 68-69 -0.13591133171664893 1106 70-71 -0.10586677010387291 1106 72-73 -0.06536027267129185 1106 74-75 -7.970080575994132E-4 1106 76-77 -0.04083440135366345 1106 78-79 0.2664185979047744 1106 80-81 0.3797830854448172 1106 82-83 0.23444262235641844 1106 84-85 0.14539466682202828 1106 86-87 0.14574783154719384 1106 88-89 0.16125273473186397 1106 90-91 0.28551997240176163 1106 92-93 0.41373429579861565 1106 94-95 0.5936063339251341 1106 96-97 0.550098722864103 1106 98-99 0.7164681778649857 1106 100-101 0.7877750693637786 1107 1 4.336150662744842E-4 1107 2 -0.011933957949640472 1107 3 0.11301971925920995 1107 4 0.12122380315823023 1107 5 0.0362366231683211 1107 6 0.08092692752844499 1107 7 0.07533511284722039 1107 8 0.16809900776758724 1107 9 0.20992970815374434 1107 10-11 0.2582061484928815 1107 12-13 0.20150307952111035 1107 14-15 0.20251440579105662 1107 16-17 0.3101261265679298 1107 18-19 0.21499406696842982 1107 20-21 0.12012620280822972 1107 22-23 0.07065042370879837 1107 24-25 0.22361129458906248 1107 26-27 0.3804299469564185 1107 28-29 0.1949602304411613 1107 30-31 0.2859502139176513 1107 32-33 0.37052320290655416 1107 34-35 0.3379593075577887 1107 36-37 0.44632448822525106 1107 38-39 0.3530060314923702 1107 40-41 0.2767201680599669 1107 42-43 0.3675939498436378 1107 44-45 0.33961139203451296 1107 46-47 0.3864534366665353 1107 48-49 0.5918282009094611 1107 50-51 0.5492935577349982 1107 52-53 0.4353037732438523 1107 54-55 0.35770017519726593 1107 56-57 0.44002742847384724 1107 58-59 0.5219179681634643 1107 60-61 0.7646387379861821 1107 62-63 0.7179341731296489 1107 64-65 0.8812603679941091 1107 66-67 0.9540880208598139 1107 68-69 0.7377967840633488 1107 70-71 0.7949566793453684 1107 72-73 0.6070766270383317 1107 74-75 0.6490397844853284 1107 76-77 0.7342046273851537 1107 78-79 0.6761931842121278 1107 80-81 0.9841057954757737 1107 82-83 0.9644114992597537 1107 84-85 0.9908688315350958 1107 86-87 1.017375147149135 1107 88-89 0.9416387106780917 1107 90-91 1.0792782111917099 1107 92-93 1.1946231401124017 1107 94-95 1.288374824676712 1107 96-97 1.224675959809204 1107 98-99 1.3589269704294864 1107 100-101 1.5298479499144584 1108 1 0.15136867270649645 1108 2 0.0391195826909545 1108 3 0.02047510849730827 1108 4 0.06003179541028203 1108 5 0.054045386892660474 1108 6 0.1263325338179726 1108 7 0.1013314498025153 1108 8 0.11458000084596875 1108 9 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quality scores pass #Quality Count 2 4927.0 3 1033.0 4 900.0 5 971.0 6 1103.0 7 1268.0 8 1572.0 9 1856.0 10 2416.0 11 3158.0 12 4040.0 13 5335.0 14 6145.0 15 7791.0 16 9760.0 17 12882.0 18 11013.0 19 7650.0 20 7517.0 21 7881.0 22 8747.0 23 9513.0 24 10897.0 25 12522.0 26 14566.0 27 17187.0 28 20601.0 29 25301.0 30 31191.0 31 38853.0 32 50234.0 33 66248.0 34 90146.0 35 128621.0 36 192127.0 37 280859.0 38 245379.0 39 33758.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.31208380260899 19.60781312076513 14.829329455202856 22.250773621423026 2 14.795947737088202 20.609544517545935 40.93949950778544 23.655008237580418 3 19.403180830204406 25.18709801524181 29.743028499521646 25.666692655032136 4 12.04360733288738 16.736474541986013 38.68361875864401 32.5362993664826 5 13.001509108940759 38.342661730061145 34.497749452938905 14.158079708059196 6 30.75174226811631 37.173775314773415 17.91080752397667 14.163674893133607 7 27.940607886668012 33.212196761131835 21.001018653964195 17.84617669823596 8 25.902036144069463 35.08667556991208 19.964144290830287 19.047143995188172 9 25.962171387961718 17.37995794231923 20.973646960138407 35.68422370958065 10-11 24.40356175148215 27.36811938924549 27.628845482283186 20.599473376989174 12-13 25.563579616248106 24.66274792806163 28.15538682129955 21.61828563439072 14-15 22.360046049963188 26.08573128346432 26.966717177932427 24.587505488640062 16-17 21.29456149025085 29.53883313102245 27.381254919722508 21.785350459004192 18-19 21.15863304310469 28.310235793329046 29.776949304343237 20.754181859223028 20-21 22.219952986781035 27.00049182947824 29.673335670524487 21.106219513216235 22-23 21.618430695399642 27.091945599421003 29.694347149729012 21.595276555450344 24-25 21.57442117992584 27.249009302328968 29.81757912294407 21.358990394801122 26-27 21.388466623566483 27.267200759495147 30.03019925098868 21.31413336594969 28-29 20.991129242190073 27.872019339817562 29.901717173372074 21.235134244620294 30-31 21.356346799728517 27.307827870165656 29.791267961747632 21.544557368358195 32-33 21.240194304413645 27.411056069682786 29.767590542259608 21.581159083643957 34-35 21.847824305946222 27.391479282615382 29.554083306479654 21.206613104958734 36-37 21.6504351776751 27.176106801644313 29.851353732393033 21.322104288287555 38-39 21.305743845879416 27.684696425841114 29.8227118175133 21.18684791076616 40-41 21.471160514230903 27.657803106280817 29.567512372520717 21.303524006967564 42-43 21.262114856594902 27.62413018041862 29.29285133357167 21.820903629414808 44-45 21.485545856313763 27.515317354765916 29.239045392670022 21.760091396250292 46-47 21.755211725732103 27.835543243151733 28.921050169242964 21.488194861873204 48-49 21.647684304770767 27.88621287407195 28.83582250408429 21.630280317072984 50-51 21.440129273086484 28.02463121269253 28.97047589710876 21.564763617112227 52-53 21.68399256977489 28.34330087131443 28.34770715066307 21.624999408247607 54-55 21.404663156028253 28.013052205545293 28.577186530246212 22.00509810818024 56-57 21.75511233728012 28.117136108278864 28.373440053233757 21.754311501207262 58-59 22.001188913040075 27.873057869049926 28.492559464763435 21.63319375314656 60-61 21.701561351659578 28.112374511815975 28.94501288780349 21.241051248720957 62-63 21.531932894252094 28.09179496663206 28.897634756828307 21.478637382287545 64-65 21.324075169495384 28.188779178231787 28.725667743550076 21.761477908722757 66-67 21.475764711363844 28.14263036757616 28.29598578632236 22.08561913473764 68-69 21.487118074008286 28.0233032184795 28.586846530725158 21.902732176787055 70-71 21.694847135246594 28.07722635166866 28.408722730545794 21.819203782538956 72-73 21.67125342186499 27.816122080493916 28.703404391636088 21.809220106005007 74-75 21.352119874871264 28.532606024687183 28.322735445484433 21.792538654957113 76-77 21.470543158020426 28.046752049661492 28.3202950799023 22.162409712415773 78-79 21.561530864359323 28.04842133389832 28.336666095128056 22.053381706614303 80-81 21.671794841924545 28.27338003783152 28.154191828217535 21.900633292026402 82-83 21.731394159837297 28.106997937083033 28.44022436906666 21.72138353401301 84-85 21.53456057314062 28.193840456398384 28.3070082107592 21.964590759701796 86-87 21.52217118146808 28.243231437869003 28.296448097377024 21.93814928328589 88-89 21.609482670444518 27.975828858240785 28.139738639792693 22.274949831522004 90-91 21.57256956312982 27.75932807908241 28.39279402455454 22.27530833323323 92-93 21.77175324356229 27.825492836189134 28.474915104809934 21.927838815438637 94-95 21.488740732543988 27.749114750470287 28.550607735450168 22.21153678153556 96-97 21.177925704618055 27.78633161819733 28.832084108638867 22.203658568545745 98-99 21.2897744212553 28.001039670945605 28.214616331934607 22.49456957586449 100-101 21.163397374835885 27.6317068225803 28.727824402793544 22.47707139979027 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1711.0 1 950.0 2 204.5 3 233.5 4 207.0 5 194.0 6 344.0 7 695.5 8 1160.5 9 1473.5 10 1459.0 11 1382.5 12 1428.5 13 1522.5 14 1647.0 15 1752.0 16 1811.0 17 1922.5 18 2095.5 19 2285.0 20 2548.5 21 2930.5 22 3452.0 23 4212.5 24 5166.5 25 6442.0 26 8275.0 27 10494.0 28 12404.0 29 14684.5 30 17679.0 31 20815.5 32 24217.5 33 27910.5 34 32423.0 35 36092.5 36 39707.5 37 43524.5 38 45247.5 39 46135.5 40 46968.0 41 48164.5 42 49297.0 43 50547.0 44 52126.0 45 53100.0 46 52903.5 47 54953.0 48 60807.5 49 59390.5 50 51329.5 51 45956.0 52 42614.0 53 40377.5 54 38017.5 55 34294.5 56 29809.0 57 26096.5 58 23137.0 59 19725.5 60 16064.5 61 12794.0 62 9993.0 63 7593.5 64 5686.0 65 4256.0 66 3210.0 67 2445.5 68 1809.5 69 1275.0 70 884.5 71 614.5 72 442.5 73 336.0 74 255.0 75 208.0 76 155.0 77 94.5 78 54.5 79 36.0 80 25.5 81 17.0 82 11.0 83 12.5 84 11.5 85 8.0 86 10.5 87 8.5 88 6.0 89 7.5 90 5.5 91 5.0 92 4.0 93 3.0 94 4.5 95 4.5 96 5.5 97 8.5 98 7.5 99 4.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30421931698184784 2 0.2625036246866781 3 0.2595966078019973 4 0.26475656277230575 5 0.26511993988289084 6 0.20138359468626385 7 0.18895609750425332 8 0.19615096429383838 9 0.19106368474564692 10-11 0.21021365847348183 12-13 0.1998574108218064 14-15 0.19549688549478517 16-17 0.194915482117849 18-19 0.19175410125575862 20-21 0.18441388362193956 22-23 0.18724822508450334 24-25 0.17834548587516832 26-27 0.17711000369917898 28-29 0.19248085547692884 30-31 0.20240105059590213 32-33 0.1941887278966788 34-35 0.2033821687944819 36-37 0.18306938831277467 38-39 0.1824879849358385 40-41 0.17976265660645024 42-43 0.18633978230804057 44-45 0.19044594365765227 46-47 0.19328028512021606 48-49 0.19858559093475855 50-51 0.2008748667314447 52-53 0.2137020787350988 54-55 0.19814953840205643 56-57 0.1756201575457801 58-59 0.1785998498525779 60-61 0.17409397368132262 62-63 0.18219728324737042 64-65 0.17576550839001412 66-67 0.17256778981686519 68-69 0.17085991739711523 70-71 0.18256066035795554 72-73 0.17885421382998748 74-75 0.1828150243353651 76-77 0.18332375229018424 78-79 0.18136151589302468 80-81 0.18336009000124276 82-83 0.17736436767658856 84-85 0.17125963221875884 86-87 0.17085991739711523 88-89 0.17147765848510987 90-91 0.17634691176695028 92-93 0.17264046523898222 94-95 0.17129596992981735 96-97 0.16926105811054076 98-99 0.17947195491798215 100-101 0.16820726448984397 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1375981.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.67178937953035 #Duplication Level Percentage of deduplicated Percentage of total 1 80.60019468616413 50.5135842532042 2 11.58665160906146 14.523123785141925 3 3.492855996053154 6.5671060595301896 4 1.4720425812981974 3.6902217045128327 5 0.7633701255614211 2.3920885863905514 6 0.4606297316525849 1.7321093714448237 7 0.2944757805142286 1.2918726865632384 8 0.21751396104528414 1.0905591322989934 9 0.16037068075603114 0.9045645774294496 >10 0.8696404129336273 9.840575368940916 >50 0.056796377750311745 2.4589500615589266 >100 0.023143727508105373 2.464095815858122 >500 8.100153955238982E-4 0.35811162032568816 >1k 0.0013885978208981113 1.7761988264513473 >5k 1.1571648507484261E-4 0.3968381503487577 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5472 0.39767990982433626 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4166 0.30276580853950746 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3880 0.2819806378140396 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2657 0.19309859656492348 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2503 0.18190658155890232 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1831 0.13306869789626455 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1830 0.13299602247414752 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1612 0.11715278045263705 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 2.1802626635106153E-4 0.0 8 0.0 0.0 0.0 2.9070168846808206E-4 0.0 9 0.0 0.0 0.0 3.6337711058510256E-4 0.0 10-11 0.0 0.0 0.0 3.6337711058510256E-4 0.0 12-13 0.0 0.0 0.0 4.3605253270212306E-4 0.0 14-15 0.0 0.0 0.0 4.3605253270212306E-4 0.0 16-17 0.0 0.0 0.0 4.3605253270212306E-4 0.0 18-19 0.0 0.0 0.0 4.3605253270212306E-4 0.0 20-21 0.0 0.0 0.0 5.087279548191436E-4 0.0 22-23 0.0 0.0 0.0 7.994296432872256E-4 0.0 24-25 7.267542211702051E-5 0.0 0.0 8.721050654042461E-4 0.0 26-27 7.267542211702051E-5 0.0 0.0 0.0010537936206967975 0.0 28-29 2.9070168846808206E-4 0.0 0.0 0.0017442101308084925 0.0 30-31 3.6337711058510256E-4 0.0 0.0 0.0030887054399733717 0.0 32-33 3.6337711058510256E-4 0.0 0.0 0.004651227015489313 0.0 34-35 3.997148216436128E-4 0.0 0.0 0.006722476545824398 0.0 36-37 4.3605253270212306E-4 0.0 0.0 0.009556818008388198 0.0 38-39 4.3605253270212306E-4 0.0 0.0 0.012500172604127528 0.0 40-41 4.3605253270212306E-4 0.0 0.0 0.015952255154686002 0.0 42-43 4.3605253270212306E-4 0.0 0.0 0.021693613501930623 0.0 44-45 4.3605253270212306E-4 0.0 0.0 0.029978611623270958 0.0 46-47 4.3605253270212306E-4 0.0 0.0 0.0492375984842814 0.0 48-49 5.814033769361641E-4 0.0 0.0 0.09022653655828096 0.0 50-51 5.814033769361641E-4 0.0 0.0 0.12263977482247211 0.0 52-53 5.814033769361641E-4 0.0 0.0 0.15468963597607815 0.0 54-55 5.814033769361641E-4 0.0 0.0 0.2005841650429766 0.0 56-57 5.814033769361641E-4 0.0 0.0 0.23495963970432732 0.0 58-59 5.814033769361641E-4 0.0 0.0 0.27071594738590143 0.0 60-61 5.814033769361641E-4 0.0 0.0 0.3392488704422517 0.0 62-63 5.814033769361641E-4 0.0 0.0 0.3887044951928842 0.0 64-65 5.814033769361641E-4 0.0 0.0 0.44175755333830913 0.0 66-67 6.540787990531846E-4 0.0 0.0 0.5563666940168506 0.0 68-69 7.267542211702051E-4 0.0 0.0 0.6784977408845034 0.0 70-71 7.267542211702051E-4 0.0 0.0 0.7907449303442418 0.0 72-73 7.267542211702051E-4 0.0 0.0 0.9229415231751019 0.0 74-75 7.267542211702051E-4 0.0 0.0 1.0462353767966273 0.0 76-77 7.267542211702051E-4 0.0 0.0 1.1467091478734082 0.0 78-79 7.267542211702051E-4 0.0 0.0 1.2862459583380876 0.0 80-81 7.267542211702051E-4 0.0 0.0 1.5080876843502926 0.0 82-83 7.267542211702051E-4 0.0 0.0 1.6897399019317856 0.0 84-85 7.267542211702051E-4 0.0 0.0 1.890033365286294 0.0 86-87 7.267542211702051E-4 0.0 0.0 2.2587157816859387 0.0 88-89 7.267542211702051E-4 0.0 0.0 2.7594857777832686 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6465 0.0 26.111008 1 TACATGG 6390 0.0 25.663965 2 ACATGGG 6435 0.0 24.376474 3 GTATCAA 10820 0.0 22.3694 1 GAGTACT 4705 0.0 21.907719 12-13 GTGTAAG 620 0.0 21.47485 1 CCATGCG 135 3.7957955E-4 21.111662 9 CATGGGA 4010 0.0 21.101475 4 AGTACTT 5060 0.0 20.323776 12-13 AGAGTAC 8335 0.0 20.288471 10-11 TCAACGC 12125 0.0 19.60313 4 GTACTTT 5215 0.0 19.536833 14-15 ATCAACG 12190 0.0 19.536175 3 CAACGCA 12210 0.0 19.428438 5 AACGCAG 12455 0.0 19.18356 6 TATCAAC 12440 0.0 19.143566 2 CATGGGG 2540 0.0 18.902744 4 GAGTACA 5130 0.0 18.631273 1 ATGGGAG 1750 0.0 18.472424 5 GTATAGG 465 0.0 18.407015 1 >>END_MODULE