FastQCFastQC Report
Thu 26 May 2016
SRR937206_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937206_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375981
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68330.4965911593256012No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53290.3872873244616023No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT47690.3465890880760708No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37100.2696258160541461No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21360.15523470164195582No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT21040.15290908813421117No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC15930.11577194743241367No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15530.11286493054773286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG60500.024.0358721
TACATGG60150.023.4535962
TATAGGG3400.022.3526842
AACGCGA753.036318E-522.16560776-77
ACATGGG60300.021.977363
GTATCAA116600.021.9676721
GAGTACT61900.020.56743412-13
AGAGTAC94550.020.54933410-11
AGTACTT63850.020.460112-13
ACGCGAA704.8556674E-420.3561778-79
TAACGCG704.8556674E-420.3561776-77
GTACTTT63750.019.78140614-15
CATGGGA39450.019.3850754
TCAACGC134900.018.8378124
AGTACAT45650.018.83332
GGTATCA91250.018.8004471
CAACGCA135200.018.7960135
ATCAACG135900.018.6991963
AACGCAG138150.018.4627446
TATCAAC137750.018.4135822
Produced by FastQC (version 0.11.5)