##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937206_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1375981 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.263116278495126 33.0 31.0 34.0 31.0 34.0 2 32.48388749553955 34.0 31.0 34.0 31.0 34.0 3 32.56779708440742 34.0 31.0 34.0 31.0 34.0 4 36.15952981908907 37.0 35.0 37.0 35.0 37.0 5 36.03137834025325 37.0 35.0 37.0 35.0 37.0 6 36.12084614540463 37.0 35.0 37.0 35.0 37.0 7 36.08793144672782 37.0 35.0 37.0 35.0 37.0 8 36.06612445956739 37.0 35.0 37.0 35.0 37.0 9 37.783684513085575 39.0 37.0 39.0 35.0 39.0 10-11 37.71038953299501 39.0 37.0 39.0 35.0 39.0 12-13 37.63337066427516 39.0 37.0 39.0 35.0 39.0 14-15 38.872375781351636 40.0 38.0 41.0 35.0 41.0 16-17 38.86388402165437 40.0 38.0 41.0 35.0 41.0 18-19 38.87992603095537 40.0 38.0 41.0 35.0 41.0 20-21 38.79988822520078 40.0 38.0 41.0 35.0 41.0 22-23 38.67409869758376 40.0 38.0 41.0 34.0 41.0 24-25 38.53292341972745 40.0 38.0 41.0 34.0 41.0 26-27 38.326988526731114 40.0 38.0 41.0 34.0 41.0 28-29 38.334329107742036 40.0 38.0 41.0 34.0 41.0 30-31 38.46117315573398 40.0 38.0 41.0 34.0 41.0 32-33 38.42423877945989 40.0 38.0 41.0 34.0 41.0 34-35 38.31682704921071 40.0 38.0 41.0 34.0 41.0 36-37 38.17091478734082 40.0 38.0 41.0 34.0 41.0 38-39 38.041168446366626 40.0 38.0 41.0 33.5 41.0 40-41 37.90627486862101 40.0 37.5 41.0 33.0 41.0 42-43 37.7029740962993 40.0 37.0 41.0 33.0 41.0 44-45 37.42557528047263 40.0 37.0 41.0 33.0 41.0 46-47 37.10738593047433 40.0 37.0 41.0 32.0 41.0 48-49 36.799148752780745 40.0 37.0 41.0 31.0 41.0 50-51 36.601829167699265 40.0 36.0 41.0 31.0 41.0 52-53 36.40284422531997 39.0 36.0 41.0 30.5 41.0 54-55 36.22021852045922 39.0 36.0 41.0 30.0 41.0 56-57 35.98571128525757 39.0 35.0 40.0 29.5 41.0 58-59 35.75703734281215 39.0 35.0 40.0 29.0 41.0 60-61 35.432454009176 38.0 35.0 40.0 28.0 41.0 62-63 35.10008532094557 38.0 34.5 40.0 28.0 41.0 64-65 34.77817607946621 37.5 34.0 40.0 27.5 41.0 66-67 34.40445761969097 37.0 34.0 39.5 26.5 41.0 68-69 33.97342005449204 36.5 34.0 39.0 26.0 40.5 70-71 33.58650046766634 36.0 33.0 39.0 26.0 40.0 72-73 33.15574597323655 35.5 33.0 38.5 25.5 40.0 74-75 32.722300671302875 35.0 32.5 37.5 25.0 39.5 76-77 31.38492137609458 34.0 30.5 36.0 23.0 39.0 78-79 32.007208675119784 35.0 32.0 36.0 24.0 39.0 80-81 31.82697035787558 35.0 32.0 36.0 24.0 38.5 82-83 31.481126556253322 35.0 32.0 36.0 22.5 37.0 84-85 31.14217165789353 34.0 31.0 35.0 21.5 37.0 86-87 30.83419284132557 34.0 31.0 35.0 20.0 36.5 88-89 30.480607653739405 34.0 31.0 35.0 19.0 36.0 90-91 30.051007245012833 34.0 31.0 35.0 17.0 36.0 92-93 29.664158153346595 34.0 30.0 35.0 9.0 35.0 94-95 29.28973728561659 34.0 30.0 35.0 2.0 35.0 96-97 28.902957598978475 34.0 29.5 35.0 2.0 35.0 98-99 28.30833056561101 34.0 29.0 35.0 2.0 35.0 100-101 26.708432020500283 32.5 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04949858537887053 1101 2 0.04989504064196382 1101 3 0.12774552784882331 1101 4 0.03298934687736477 1101 5 -0.17017166321090116 1101 6 -0.17792423077428055 1101 7 -0.029407022490083534 1101 8 0.02380238404196433 1101 9 0.031215690905327165 1101 10-11 0.07124164943126488 1101 12-13 0.08375388014583507 1101 14-15 0.04378323944553841 1101 16-17 0.13602829584611698 1101 18-19 0.07302449784817355 1101 20-21 1.6930874974008248E-4 1101 22-23 -0.048283449932789324 1101 24-25 0.008944685484166826 1101 26-27 0.01744720915315412 1101 28-29 0.09783639688436097 1101 30-31 0.13520024758744142 1101 32-33 0.10825515753396076 1101 34-35 0.09491028339970597 1101 36-37 0.22448660898559325 1101 38-39 0.14132476853000497 1101 40-41 0.17182511939908096 1101 42-43 0.10125309475788669 1101 44-45 -0.0010048899851327064 1101 46-47 -0.09890066820407384 1101 48-49 -0.06028154561192167 1101 50-51 0.005926566561477387 1101 52-53 -0.06328293027185339 1101 54-55 0.06026172510369321 1101 56-57 -0.08939030295599792 1101 58-59 -0.10374239616981384 1101 60-61 -0.17602288302885682 1101 62-63 -0.15568414952752363 1101 64-65 -0.07546662830924333 1101 66-67 -0.0833988066609237 1101 68-69 -0.02463465173340751 1101 70-71 -0.04331591350619135 1101 72-73 -0.19091871636456403 1101 74-75 -0.20229493849578972 1101 76-77 -0.16483661646207892 1101 78-79 -0.04229592532918858 1101 80-81 -0.16610677696719023 1101 82-83 -0.1220333075754958 1101 84-85 -0.1252792532482907 1101 86-87 -0.2312468685884994 1101 88-89 -0.32777466563201685 1101 90-91 -0.17877442602037874 1101 92-93 -0.05443400967102008 1101 94-95 -0.323013514487446 1101 96-97 -0.2381075996709967 1101 98-99 -0.23215447241284792 1101 100-101 0.10042237370933549 1102 1 0.2267562779824175 1102 2 0.1823843834277028 1102 3 0.16507860358436233 1102 4 0.08660546534373026 1102 5 0.09126362160460388 1102 6 0.1489165648451234 1102 7 0.16180388391425993 1102 8 0.23037398996743264 1102 9 0.2638640645509227 1102 10-11 0.215151518825607 1102 12-13 0.2003435358055654 1102 14-15 0.3043468061732071 1102 16-17 0.2674326868151624 1102 18-19 0.18006103201297918 1102 20-21 0.14571993297716546 1102 22-23 0.17241131278208144 1102 24-25 0.2068225447749441 1102 26-27 0.03798115213459852 1102 28-29 0.0600575338566216 1102 30-31 0.12297347566344996 1102 32-33 0.1803625042587953 1102 34-35 0.329116408145012 1102 36-37 0.364793865908986 1102 38-39 0.31548466878241754 1102 40-41 0.29266442309793206 1102 42-43 0.30852772799408257 1102 44-45 0.5109959472076895 1102 46-47 0.6146228581666051 1102 48-49 0.8099312051210745 1102 50-51 0.78118537184438 1102 52-53 0.8721745115316182 1102 54-55 0.9728553949956975 1102 56-57 0.9507056879994593 1102 58-59 0.9362766732223733 1102 60-61 0.8537545274335514 1102 62-63 0.8163076359650745 1102 64-65 0.7759570467168331 1102 66-67 0.8597232428157398 1102 68-69 0.6795792188344336 1102 70-71 0.7224110578772027 1102 72-73 0.7796816832697999 1102 74-75 0.6993188043464826 1102 76-77 0.7911984168378048 1102 78-79 0.8247052606939782 1102 80-81 0.7288936791955649 1102 82-83 0.6834111291171645 1102 84-85 0.7160594681722188 1102 86-87 0.7642979417998461 1102 88-89 0.6393470904693821 1102 90-91 0.5300080487430883 1102 92-93 0.7170273748097884 1102 94-95 0.6763181623489665 1102 96-97 1.0182322576842253 1102 98-99 0.9730214543578235 1102 100-101 0.9435281444365664 1103 1 0.08788004154176576 1103 2 0.039256289110021214 1103 3 0.04877513947320722 1103 4 0.034887932023536905 1103 5 -0.01026258764016319 1103 6 0.018887851940249334 1103 7 0.0643801171912699 1103 8 0.04216934124407601 1103 9 0.009111265014112746 1103 10-11 0.07616017157555888 1103 12-13 0.09541812722889631 1103 14-15 0.049803645527681795 1103 16-17 0.0443622317577379 1103 18-19 0.06224835846532528 1103 20-21 0.09597109942702531 1103 22-23 0.10107939305014924 1103 24-25 0.04842301954986539 1103 26-27 0.13449813536234245 1103 28-29 -0.09289576355349993 1103 30-31 0.04723475585574022 1103 32-33 0.08109315296919561 1103 34-35 0.05870682330243682 1103 36-37 0.061779716046146405 1103 38-39 0.08672042143749081 1103 40-41 0.06716654026015334 1103 42-43 0.08235290211823809 1103 44-45 0.04030213014468842 1103 46-47 -0.059174071881471946 1103 48-49 -0.08688491374646645 1103 50-51 -0.23044672791018428 1103 52-53 -0.07762707478681108 1103 54-55 -0.15505043826362197 1103 56-57 0.016399083878873455 1103 58-59 -0.05839068512558043 1103 60-61 -0.1848537142717106 1103 62-63 -0.10310984235349707 1103 64-65 -0.05437305068139153 1103 66-67 -0.10518358794354299 1103 68-69 -0.028640036002222757 1103 70-71 0.08554907002024947 1103 72-73 0.015078142811965733 1103 74-75 -0.13696618104931702 1103 76-77 -0.33904774877158417 1103 78-79 -0.06571248863050272 1103 80-81 -0.1218906942452449 1103 82-83 -0.13119490794927557 1103 84-85 -0.1107925294386618 1103 86-87 0.1035159658656255 1103 88-89 0.21397529589567554 1103 90-91 0.22845317614445548 1103 92-93 0.16497327577866372 1103 94-95 0.16565078265596256 1103 96-97 0.2558428850800638 1103 98-99 0.18581960374192263 1103 100-101 0.5202740838265321 1104 1 0.09979597976504806 1104 2 0.01930437416802988 1104 3 0.04596555151188397 1104 4 0.037031340784935196 1104 5 -9.604141544770073E-4 1104 6 0.008539640224547895 1104 7 0.051116991236632714 1104 8 0.15164288856846042 1104 9 0.08654014791296305 1104 10-11 0.04295197498576897 1104 12-13 0.09640237657764317 1104 14-15 0.1509153510795187 1104 16-17 0.08052716890609446 1104 18-19 0.09402894671828932 1104 20-21 0.05817379509529985 1104 22-23 0.0597004249206563 1104 24-25 0.11979221933251694 1104 26-27 0.03228373592935441 1104 28-29 -0.05976228398014172 1104 30-31 -0.11219975990764652 1104 32-33 0.08158442210520889 1104 34-35 0.025769166952805733 1104 36-37 0.041727988295939156 1104 38-39 -0.013177370246900466 1104 40-41 0.10171660123152293 1104 42-43 0.19664522170325682 1104 44-45 0.1540117330494084 1104 46-47 0.17470216708066033 1104 48-49 0.021535370801792908 1104 50-51 0.012697843242655438 1104 52-53 -0.0379415022434344 1104 54-55 0.09321120285666495 1104 56-57 -0.06473199866612589 1104 58-59 -0.0782730162812797 1104 60-61 -0.05390862576531674 1104 62-63 0.021756170833960198 1104 64-65 -0.004700683847843834 1104 66-67 -0.05482431886988337 1104 68-69 -0.09952557143424912 1104 70-71 -0.35045552022820914 1104 72-73 -0.17007594725075137 1104 74-75 -0.28520131949253624 1104 76-77 -0.2571578987645715 1104 78-79 -0.2340320554010873 1104 80-81 -0.14615699405706195 1104 82-83 -0.1692272001494679 1104 84-85 -0.1775499972428527 1104 86-87 -0.2705371908237524 1104 88-89 -0.07459969280170853 1104 90-91 0.010076630519353813 1104 92-93 0.10469527524404754 1104 94-95 0.017483516922325748 1104 96-97 0.16532996892244256 1104 98-99 0.3588273305841163 1104 100-101 0.5792332244553648 1105 1 -0.022829218995937595 1105 2 -0.048771883935302185 1105 3 -0.05262210222140595 1105 4 0.02192605669032588 1105 5 -0.09803621715988697 1105 6 -0.027451663327973108 1105 7 -0.055267933451823126 1105 8 0.035213853541350204 1105 9 0.041070025146716205 1105 10-11 0.02409075560984064 1105 12-13 -0.018443733106465743 1105 14-15 0.03556180170139811 1105 16-17 0.03472316073857229 1105 18-19 -0.06304429980484372 1105 20-21 0.016306990025974244 1105 22-23 -0.08887857864629467 1105 24-25 -0.11015950502579841 1105 26-27 -0.06723505035864008 1105 28-29 -0.1547080523959039 1105 30-31 -0.08763934813769225 1105 32-33 0.07389018179367213 1105 34-35 0.06755993871292532 1105 36-37 0.005360925925494087 1105 38-39 -0.0478188070781016 1105 40-41 -0.07538756550402326 1105 42-43 -0.25129565303241463 1105 44-45 -0.09993456098792564 1105 46-47 0.13152902160469893 1105 48-49 0.13240185300504237 1105 50-51 0.2627609288506463 1105 52-53 0.11410583234830796 1105 54-55 0.04709700082624835 1105 56-57 0.13483395031221335 1105 58-59 0.12011111060839852 1105 60-61 0.018559382393391388 1105 62-63 0.15704642523700585 1105 64-65 0.23070119626856922 1105 66-67 0.08945825176120081 1105 68-69 0.04338013514109207 1105 70-71 -0.045971318076581724 1105 72-73 0.06609618334583445 1105 74-75 0.03945595210175412 1105 76-77 -0.02287778544188157 1105 78-79 0.006882790598400135 1105 80-81 0.12080587193588599 1105 82-83 0.013833460106159379 1105 84-85 0.018468832571461036 1105 86-87 0.08187003476487575 1105 88-89 0.19950314169801686 1105 90-91 0.03788498691061548 1105 92-93 0.17245693175640753 1105 94-95 0.2551140545439381 1105 96-97 0.19350471770949085 1105 98-99 0.19213587044705704 1105 100-101 0.22044966924801912 1106 1 -0.18809299975897176 1106 2 -0.07009250734236616 1106 3 -0.12418800389163209 1106 4 -0.011948552445083749 1106 5 -0.11949270955527425 1106 6 -0.017759804157726933 1106 7 -0.0439537522419613 1106 8 -0.02473872354982376 1106 9 -0.06530805887667412 1106 10-11 -0.14608746821965468 1106 12-13 -0.269789974765672 1106 14-15 -0.0902357048710698 1106 16-17 -0.028726498836270764 1106 18-19 -0.10156026305999433 1106 20-21 -0.29670756069349835 1106 22-23 -0.1633096948061734 1106 24-25 -0.13115493064119477 1106 26-27 0.011877370035442425 1106 28-29 -0.04949045164924115 1106 30-31 -0.14524915015881845 1106 32-33 -0.18116119173757994 1106 34-35 -0.07220158033324253 1106 36-37 -0.11929385297668205 1106 38-39 -0.0870654521369616 1106 40-41 -0.16245430458268117 1106 42-43 -0.12129180469771228 1106 44-45 -0.00979031212533954 1106 46-47 0.003745010065095755 1106 48-49 -0.18997472719568265 1106 50-51 -0.0542324216747474 1106 52-53 0.024122155926477262 1106 54-55 -0.001089206078290772 1106 56-57 -0.04112430239715792 1106 58-59 -0.10729478371255396 1106 60-61 -0.005128719536145354 1106 62-63 0.09347093245137472 1106 64-65 0.12186762767344561 1106 66-67 0.4145199814429006 1106 68-69 0.20681643941196626 1106 70-71 0.027891425681843884 1106 72-73 0.08453731057980463 1106 74-75 0.12609790759616857 1106 76-77 -0.015078249326396787 1106 78-79 -0.02064573320433638 1106 80-81 -0.04973537684679741 1106 82-83 -0.02618072893932677 1106 84-85 -0.129717721725374 1106 86-87 -0.10183873314657532 1106 88-89 -0.07207908741029101 1106 90-91 0.08371818997183667 1106 92-93 0.05715086563067118 1106 94-95 -0.09290007737481432 1106 96-97 0.07233409057761264 1106 98-99 0.27467106997182356 1106 100-101 0.2661031932920075 1107 1 -0.08559575984101286 1107 2 -0.06193792836326395 1107 3 0.010434473225565455 1107 4 -0.024194406128572155 1107 5 -0.0548886090103764 1107 6 0.008202748231887824 1107 7 -0.03241435169621809 1107 8 0.04028373470730173 1107 9 -0.037400362892867633 1107 10-11 -0.08003090322016959 1107 12-13 -0.0054618224882574395 1107 14-15 -0.0029933375018558195 1107 16-17 0.0336771280493835 1107 18-19 -0.04743108842342281 1107 20-21 0.01080453751627175 1107 22-23 -0.07333921160101653 1107 24-25 -0.1423375084199634 1107 26-27 -0.03614925621527476 1107 28-29 -0.14035019472886034 1107 30-31 -0.11524167406255259 1107 32-33 -0.14926511227278638 1107 34-35 -0.07359990202754574 1107 36-37 -0.013016009912369952 1107 38-39 0.035180509432464646 1107 40-41 0.013426753962228588 1107 42-43 -0.002499392036376946 1107 44-45 -0.06120635508472816 1107 46-47 -0.03466118690347031 1107 48-49 -0.060800689987821954 1107 50-51 -0.028372560719745366 1107 52-53 -0.09428326586161262 1107 54-55 -0.3024420158877632 1107 56-57 -0.08259525930151312 1107 58-59 -0.012821860486987191 1107 60-61 -0.12671417938798868 1107 62-63 -0.07294013776519392 1107 64-65 -0.05154464087624433 1107 66-67 -0.15452362565567768 1107 68-69 -0.17320320382120258 1107 70-71 -0.14323785644109677 1107 72-73 -0.07385963217231506 1107 74-75 0.003825773696128465 1107 76-77 -0.012209282398234933 1107 78-79 0.021025412060883042 1107 80-81 0.10343928689869131 1107 82-83 0.11079126426945507 1107 84-85 1.439884746012865E-4 1107 86-87 -0.005849213266991171 1107 88-89 0.024157571194233896 1107 90-91 0.05309176822490613 1107 92-93 0.042094583132527674 1107 94-95 0.22316178202742876 1107 96-97 0.2335298743949572 1107 98-99 0.17607730330194826 1107 100-101 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90-91 -0.2830783037716209 2316 92-93 -0.28637962403643513 2316 94-95 -0.44670152381598527 2316 96-97 -0.43901931220407064 2316 98-99 -0.5245311021815873 2316 100-101 -0.7474841559361884 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 5.0 5 20.0 6 36.0 7 97.0 8 219.0 9 466.0 10 858.0 11 1563.0 12 2384.0 13 3475.0 14 4529.0 15 6467.0 16 8998.0 17 12915.0 18 11495.0 19 6550.0 20 5270.0 21 5277.0 22 5429.0 23 5704.0 24 6599.0 25 8020.0 26 10027.0 27 12503.0 28 15948.0 29 20362.0 30 25593.0 31 33227.0 32 43503.0 33 58454.0 34 81758.0 35 119981.0 36 192733.0 37 321095.0 38 308551.0 39 35861.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.819926302288586 20.07092023255205 15.28645840907174 21.822695056087625 2 15.402828963481326 21.198621201891594 40.033910351959804 23.364639482667275 3 19.697146980953953 25.122439917411647 30.21422534177434 24.966187759860055 4 12.6384012569941 17.19100772467062 38.24166176713196 31.928929251203325 5 13.037865842534439 38.35056776595381 34.529458923370676 14.082107468141073 6 30.9625641633133 37.431839538481995 17.453947401889998 14.151648896314702 7 28.050605349928524 33.453296230107824 20.730300781769515 17.76579763819413 8 26.195056472436757 35.12504896506565 19.66982102223795 19.01007354025964 9 26.022306994064596 17.272331522019563 20.882047063149855 35.82331442076598 10-11 24.592854116444922 27.458046295697397 27.323742115625144 20.62535747223254 12-13 25.832030243553934 24.624381267050246 28.16839784097132 21.3751906484245 14-15 22.48693840976002 26.02325177455212 27.086929252656834 24.40288056303103 16-17 21.242335468294986 29.452586917987965 27.591987098659065 21.713090515057985 18-19 21.116170935499838 27.96677425051654 30.286246685092312 20.630808128891314 20-21 22.057717366736895 26.679692524824105 30.377018287316464 20.885571821122532 22-23 21.5657196803835 26.717208293982065 30.388386593665594 21.328685431968843 24-25 21.638172994044965 26.816053745045345 30.511279976860095 21.0344932840496 26-27 21.478746950755312 26.875651138427077 30.720324894228046 20.925277016589565 28-29 20.966769589233937 27.519134912626043 30.623055212258265 20.891040285881754 30-31 21.360224888460586 26.964630795271123 30.43011080902141 21.245033507246884 32-33 21.28052640261748 26.939143781781873 30.4781461371923 21.30218367840835 34-35 21.80513393716919 26.951316914986474 30.336029349242466 20.90751979860187 36-37 21.666895594911832 26.64593957602489 30.605017380377724 21.08214744868555 38-39 21.332899217865403 27.235609871669457 30.53206519290279 20.899425717562345 40-41 21.46047074777922 27.311241943021013 30.23101336428337 20.99727394491639 42-43 21.25040566554407 27.242190234153966 29.92385298862392 21.58355111167804 44-45 21.464722259973065 27.1247931475798 29.897542189899422 21.51294240254771 46-47 21.824574612585494 27.590824291905193 29.4565840662044 21.12801702930491 48-49 21.749101186716967 27.58439251704784 29.289394257624195 21.377112038611 50-51 21.540958777773824 27.81408318864868 29.379402767916122 21.26555526566137 52-53 21.822203423458387 28.15384995534082 28.63656151229272 21.38738510890807 54-55 21.511089179283726 27.788646790907723 28.895348118905712 21.80491591090284 56-57 21.820541126658 27.90343762014156 28.72681381501634 21.549207438184105 58-59 22.149070372337988 27.64064329376641 28.81856653543908 21.391719798456517 60-61 21.81069360696114 27.860086730848753 29.337287360799312 20.99193230139079 62-63 21.60269654886223 27.861285875313683 29.259306632867748 21.276710942956335 64-65 21.43968557705375 28.040503466254258 29.00243535339514 21.51737560329685 66-67 21.625698319962268 27.838683819035293 28.61427592386813 21.921341937134304 68-69 21.62511691658533 27.78930086970678 28.831575435998026 21.75400677770987 70-71 21.874030237336125 27.826946738363397 28.7424390307715 21.55658399352898 72-73 21.845178094755667 27.503613785364767 29.013045965024226 21.638162154855337 74-75 21.51926516427189 28.22171963130305 28.55893358992602 21.700081614499037 76-77 21.553131910978422 27.92218787904775 28.62197951861254 21.90270069136129 78-79 21.677370545087467 27.793189004790037 28.594508209052304 21.93493224107019 80-81 21.785547910908655 28.016011849000822 28.475247841358275 21.723192398732248 82-83 21.857890535035352 27.89771903476409 28.654709807485528 21.58968062271503 84-85 21.547951278361605 28.007856218840388 28.59162197124958 21.852570531548423 86-87 21.44677143071016 28.05333067825791 28.452464096524587 22.047433794507338 88-89 21.517702642696374 27.806197905348984 28.411656847005883 22.264442604948762 90-91 21.54637144690776 27.491298290038586 28.600262087000385 22.36206817605327 92-93 21.70736892214274 27.55381291800741 28.706945577502047 22.031872582347802 94-95 21.454983753409383 27.433045950489145 28.83419175119424 22.277778544907235 96-97 21.084993179411633 27.499980014258917 29.11170285054808 22.303323955781366 98-99 21.15911799761621 27.65331114276578 28.50649728189773 22.68107357772028 100-101 20.864860307339843 27.689768761002643 28.678934384644343 22.766436547013168 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 175.0 1 133.5 2 135.5 3 207.5 4 198.5 5 192.5 6 357.0 7 764.5 8 1301.0 9 1622.5 10 1579.5 11 1512.0 12 1601.5 13 1717.5 14 1915.0 15 2117.5 16 2186.5 17 2198.0 18 2376.0 19 2603.5 20 2813.0 21 3175.0 22 3713.5 23 4463.5 24 5371.5 25 6629.5 26 8454.0 27 10496.5 28 12324.0 29 14675.0 30 17580.0 31 20352.0 32 23830.0 33 27619.5 34 31888.0 35 35423.0 36 38976.5 37 43063.5 38 45289.0 39 46298.5 40 46891.0 41 48030.0 42 49156.0 43 50642.5 44 52267.5 45 53319.5 46 53032.5 47 55256.0 48 62061.0 49 59936.5 50 51142.5 51 46153.0 52 42729.0 53 40713.0 54 38492.5 55 34165.5 56 29423.0 57 25981.5 58 23030.0 59 19597.5 60 15974.0 61 12707.0 62 9994.5 63 7534.5 64 5486.0 65 4019.0 66 2992.5 67 2361.5 68 1725.5 69 1151.0 70 745.5 71 499.5 72 392.5 73 338.5 74 266.0 75 205.5 76 145.0 77 72.0 78 42.0 79 32.0 80 18.0 81 7.5 82 4.5 83 0.5 84 0.0 85 1.0 86 1.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04600354220007398 2 0.0 3 0.0 4 0.0 5 0.010901313317553077 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006141073168888233 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0014171707312819 24-25 2.1802626635106156E-4 26-27 0.0018532232639840232 28-29 0.001998574108218064 30-31 7.267542211702051E-4 32-33 0.0 34-35 0.0 36-37 2.9070168846808206E-4 38-39 2.1802626635106156E-4 40-41 0.0 42-43 0.01130102813919669 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.9070168846808206E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.723902437606333E-4 84-85 7.267542211702051E-5 86-87 0.0 88-89 0.0 90-91 0.007412893055936093 92-93 0.002434626640920187 94-95 0.0 96-97 0.0 98-99 0.0015261838644574305 100-101 0.01271819887047859 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1375981.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.11215794533534 #Duplication Level Percentage of deduplicated Percentage of total 1 79.52219506020468 48.59772944678994 2 12.340511834095679 15.083106166630703 3 3.609816929087855 6.61811106972487 4 1.5292606357381322 3.7382567004325065 5 0.8018365286337608 2.4500980292102894 6 0.4733580980224887 1.7356760910632312 7 0.3264402959022211 1.396462964602898 8 0.2293073117301371 1.1210771721977908 9 0.17109456361729605 0.9410362195831581 >10 0.910077833637472 10.227553179013455 >50 0.05670898929452746 2.4092086314302374 >100 0.027369029149053932 2.965391145616963 >500 4.7597432630178414E-4 0.2475373329405421 >1k 0.0013089293973299063 1.5843407381831935 >5k 2.3798716315089207E-4 0.8844151125801624 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6833 0.4965911593256012 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5329 0.3872873244616023 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4769 0.3465890880760708 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3710 0.2696258160541461 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2136 0.15523470164195582 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2104 0.15290908813421117 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1593 0.11577194743241367 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1553 0.11286493054773286 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 7.267542211702051E-5 0.0 0.0 0.0 0.0 8 7.267542211702051E-5 0.0 0.0 0.0 0.0 9 7.267542211702051E-5 0.0 0.0 0.0 0.0 10-11 7.267542211702051E-5 0.0 0.0 7.267542211702051E-5 0.0 12-13 7.267542211702051E-5 0.0 0.0 2.1802626635106153E-4 0.0 14-15 7.267542211702051E-5 0.0 0.0 2.1802626635106153E-4 0.0 16-17 7.267542211702051E-5 0.0 0.0 2.1802626635106153E-4 0.0 18-19 7.267542211702051E-5 0.0 0.0 2.9070168846808206E-4 0.0 20-21 7.267542211702051E-5 0.0 0.0 3.6337711058510256E-4 0.0 22-23 7.267542211702051E-5 0.0 0.0 5.814033769361641E-4 0.0 24-25 7.267542211702051E-5 0.0 0.0 6.540787990531846E-4 0.0 26-27 7.267542211702051E-5 0.0 0.0 7.630919322287154E-4 0.0 28-29 1.0901313317553078E-4 0.0 0.0 0.0014535084423404102 0.0 30-31 1.4535084423404103E-4 0.0 0.0 0.0027616660404467795 0.0 32-33 1.4535084423404103E-4 0.0 0.0 0.004469538460196761 0.0 34-35 1.4535084423404103E-4 0.0 0.0 0.006649801123707376 0.0 36-37 2.1802626635106153E-4 0.0 0.0 0.009738506563680749 0.0 38-39 2.9070168846808206E-4 0.0 0.0 0.012899887425771141 0.0 40-41 3.6337711058510256E-4 0.0 0.0 0.016533658531622166 0.0 42-43 3.6337711058510256E-4 0.0 0.0 0.022238679167808275 0.0 44-45 3.6337711058510256E-4 0.0 0.0 0.030378326444914575 0.0 46-47 3.6337711058510256E-4 0.0 0.0 0.049564637883807994 0.0 48-49 3.6337711058510256E-4 0.0 0.0 0.09080793993521713 0.0 50-51 3.6337711058510256E-4 0.0 0.0 0.12300315193305722 0.0 52-53 3.6337711058510256E-4 0.0 0.0 0.15548906561936537 0.0 54-55 3.6337711058510256E-4 0.0 0.0 0.20141993239732234 0.0 56-57 4.3605253270212306E-4 0.0 0.0 0.23550470537020496 0.0 58-59 4.3605253270212306E-4 0.0 0.0 0.2715880524513057 0.0 60-61 4.3605253270212306E-4 0.0 0.0 0.34033900177400706 0.0 62-63 4.3605253270212306E-4 0.0 0.0 0.38968561339146396 0.0 64-65 5.087279548191436E-4 0.0 0.0 0.4425933206926549 0.0 66-67 5.087279548191436E-4 0.0 0.0 0.557965553303425 0.0 68-69 5.087279548191436E-4 0.0 0.0 0.680096600171078 0.0 70-71 5.450656658776539E-4 0.0 0.0 0.7923437896308161 0.0 72-73 5.814033769361641E-4 0.0 0.0 0.924794746439086 0.0 74-75 5.814033769361641E-4 0.0 0.0 1.0490333805481327 0.0 76-77 5.814033769361641E-4 0.0 0.0 1.1511423486225465 0.0 78-79 5.814033769361641E-4 0.0 0.0 1.2932954742834384 0.0 80-81 5.814033769361641E-4 0.0 0.0 1.5166270464490426 0.0 82-83 5.814033769361641E-4 0.0 0.0 1.6999144610281682 0.0 84-85 5.814033769361641E-4 0.0 0.0 1.9026788887346555 0.0 86-87 5.814033769361641E-4 0.0 0.0 2.2753221156396783 0.0 88-89 5.814033769361641E-4 0.0 0.0 2.78335965394871 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6050 0.0 24.035872 1 TACATGG 6015 0.0 23.453596 2 TATAGGG 340 0.0 22.352684 2 AACGCGA 75 3.036318E-5 22.165607 76-77 ACATGGG 6030 0.0 21.97736 3 GTATCAA 11660 0.0 21.967672 1 GAGTACT 6190 0.0 20.567434 12-13 AGAGTAC 9455 0.0 20.549334 10-11 AGTACTT 6385 0.0 20.4601 12-13 ACGCGAA 70 4.8556674E-4 20.35617 78-79 TAACGCG 70 4.8556674E-4 20.35617 76-77 GTACTTT 6375 0.0 19.781406 14-15 CATGGGA 3945 0.0 19.385075 4 TCAACGC 13490 0.0 18.837812 4 AGTACAT 4565 0.0 18.8333 2 GGTATCA 9125 0.0 18.800447 1 CAACGCA 13520 0.0 18.796013 5 ATCAACG 13590 0.0 18.699196 3 AACGCAG 13815 0.0 18.462744 6 TATCAAC 13775 0.0 18.413582 2 >>END_MODULE