FastQCFastQC Report
Thu 26 May 2016
SRR937205_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937205_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501309
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57860.38539701020909084No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46630.3105956202220862No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41360.2754929198452817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28810.19189920262917226No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27590.18377296079621183No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21750.14487357366138484No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20430.1360812464322801No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC17990.11982876276635923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG73400.026.4936771
TACATGG72850.025.7078362
ACATGGG74300.024.5655653
GTATCAA113800.022.6867641
GAGTACT50450.022.40798212-13
AGTACTT52000.021.23765812-13
CATGGGA48400.020.9200214
GTACTTT53300.020.76283614-15
GGACCGT1856.5485237E-620.5426276
AGAGTAC88150.020.20706210-11
CAACGCA128750.019.9719225
TCAACGC129000.019.899034
ATCAACG129800.019.7744053
CATGGGG27950.019.7289524
AACGCAG131150.019.6684116
TATCAAC132900.019.3137952
TAGCCCT8950.019.1208574
GTGTACG1559.5638505E-418.4049451
GAGTACA57000.018.2677151
GGTATCA87100.018.1232011
Produced by FastQC (version 0.11.5)