##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937205_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1501309 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.67870771440123 33.0 31.0 34.0 30.0 34.0 2 31.772034271425802 33.0 31.0 34.0 30.0 34.0 3 31.823483373509383 33.0 31.0 34.0 30.0 34.0 4 35.448566550923225 37.0 35.0 37.0 33.0 37.0 5 35.44192634560906 37.0 35.0 37.0 33.0 37.0 6 35.51043056426092 37.0 35.0 37.0 33.0 37.0 7 35.45246448266146 37.0 35.0 37.0 33.0 37.0 8 35.440678767662085 37.0 35.0 37.0 33.0 37.0 9 37.04886868725892 39.0 37.0 39.0 33.0 39.0 10-11 36.95436149386968 39.0 37.0 39.0 33.0 39.0 12-13 36.852685556404445 39.0 37.0 39.0 33.0 39.0 14-15 38.00280788298745 40.0 38.0 41.0 33.0 41.0 16-17 37.95407507714934 40.0 38.0 41.0 33.0 41.0 18-19 37.91736944226672 40.0 38.0 41.0 33.0 41.0 20-21 37.85529394681575 40.0 38.0 41.0 33.0 41.0 22-23 37.72186838285789 40.0 37.5 41.0 32.0 41.0 24-25 37.5823174975971 40.0 37.0 41.0 32.0 41.0 26-27 37.41864466275763 40.0 37.0 41.0 32.0 41.0 28-29 37.24724290602401 39.0 37.0 41.0 31.5 41.0 30-31 37.26209361297374 39.0 37.0 41.0 32.0 41.0 32-33 37.12310989942777 39.0 36.0 41.0 31.0 41.0 34-35 36.93173423991996 39.0 36.0 41.0 31.0 41.0 36-37 36.95451935610857 39.0 36.0 41.0 31.0 41.0 38-39 36.94276394799472 39.5 36.0 41.0 31.0 41.0 40-41 36.81315538639947 39.5 36.0 41.0 30.5 41.0 42-43 36.59761115133527 39.0 36.0 41.0 30.0 41.0 44-45 36.38804803008574 39.0 36.0 41.0 30.0 41.0 46-47 36.095874000622125 39.0 35.5 41.0 29.5 41.0 48-49 35.81161772826247 39.0 35.0 41.0 28.0 41.0 50-51 34.90178570833852 38.0 34.0 39.5 26.5 40.5 52-53 35.051444106443114 38.5 34.5 40.0 27.0 40.5 54-55 35.43003172564742 39.0 35.0 40.0 27.0 41.0 56-57 35.22880066661826 39.0 35.0 40.0 26.5 41.0 58-59 34.99790449534373 38.0 34.5 40.0 26.0 41.0 60-61 34.72231799050029 38.0 34.0 40.0 26.0 41.0 62-63 34.43341510641713 38.0 34.0 40.0 25.5 41.0 64-65 34.0948765377414 37.5 34.0 40.0 24.5 41.0 66-67 33.701453531551465 37.0 33.0 39.5 23.0 41.0 68-69 33.21442787594026 36.0 33.0 39.0 22.0 41.0 70-71 32.78988302874358 36.0 32.0 39.0 21.5 40.0 72-73 32.38113273150297 35.0 32.0 38.5 20.5 40.0 74-75 31.92212995459296 35.0 31.0 37.5 18.5 39.5 76-77 31.48569947958748 35.0 31.0 37.0 18.0 39.0 78-79 31.010756280019635 35.0 31.0 36.5 14.5 39.0 80-81 30.55150671847035 34.5 30.5 36.0 9.5 38.0 82-83 30.09002543780128 34.0 30.0 35.5 7.0 37.0 84-85 29.614220656773522 34.0 29.5 35.0 3.5 37.0 86-87 29.203681920244264 34.0 29.0 35.0 2.0 36.0 88-89 28.812104636687053 34.0 29.0 35.0 2.0 36.0 90-91 28.381105422001735 34.0 29.0 35.0 2.0 35.5 92-93 27.935315448052332 34.0 27.5 35.0 2.0 35.0 94-95 27.401445671743794 33.0 26.5 35.0 2.0 35.0 96-97 26.782204063254134 33.0 25.5 35.0 2.0 35.0 98-99 26.091884815184613 33.0 24.5 35.0 2.0 35.0 100-101 24.239152632802444 30.5 12.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09816327210988263 1101 2 0.03216593351243091 1101 3 0.11784498556370693 1101 4 0.11221300377365395 1101 5 0.17237593856561517 1101 6 0.05925890052532168 1101 7 0.14733928020331177 1101 8 0.062037539304952816 1101 9 0.12946453870571162 1101 10-11 0.2872724878958337 1101 12-13 0.06751322095612267 1101 14-15 0.2654041837797152 1101 16-17 0.19629540167215254 1101 18-19 0.30104024864822776 1101 20-21 0.21996924086428749 1101 22-23 0.1582780577004428 1101 24-25 0.19369372337036594 1101 26-27 0.23531508969880832 1101 28-29 0.210151728372864 1101 30-31 0.20696273242553787 1101 32-33 0.13860795432941586 1101 34-35 -0.03815230986800344 1101 36-37 0.014073246098220693 1101 38-39 0.3695045833173012 1101 40-41 0.28090659506094084 1101 42-43 0.16444744589142601 1101 44-45 0.18079281698971528 1101 46-47 0.41810013746138 1101 48-49 0.5726455649546907 1101 50-51 0.48327148685302035 1101 52-53 0.332546161395193 1101 54-55 0.35922083645399994 1101 56-57 0.4538188368880398 1101 58-59 0.4799589715256758 1101 60-61 0.48787203003323754 1101 62-63 0.621820747053043 1101 64-65 0.4800686611849798 1101 66-67 0.5772820367778095 1101 68-69 0.5116650071489133 1101 70-71 0.4136851442542522 1101 72-73 0.4779498824310835 1101 74-75 0.47596496969818247 1101 76-77 0.5958669664862484 1101 78-79 0.6357980357250099 1101 80-81 0.8129027353303009 1101 82-83 0.8141148758114447 1101 84-85 0.7089982197986302 1101 86-87 0.7731395947786197 1101 88-89 0.7306905783285877 1101 90-91 0.7071016010926172 1101 92-93 0.8315357351980524 1101 94-95 0.9396853851746911 1101 96-97 0.8254663815949321 1101 98-99 0.7571186252052868 1101 100-101 1.0547138578221755 1102 1 0.01785588522924897 1102 2 -0.07788617997917413 1102 3 0.05558408722637509 1102 4 -0.13751352447516751 1102 5 -0.16368891377948813 1102 6 -0.1045567996980239 1102 7 -0.2475972736886547 1102 8 -0.11258831891326082 1102 9 -0.02642891815590076 1102 10-11 -0.04568606947037068 1102 12-13 0.06364044405150082 1102 14-15 0.08540699625385884 1102 16-17 0.11898412695960303 1102 18-19 0.17932497029600825 1102 20-21 0.19551163477611055 1102 22-23 0.24205802295811196 1102 24-25 0.18137012340346104 1102 26-27 0.26637919756546324 1102 28-29 0.06748053968893686 1102 30-31 0.19258288297562132 1102 32-33 0.13357594175599274 1102 34-35 0.04664841806647502 1102 36-37 0.07621056101260848 1102 38-39 0.2459709577072431 1102 40-41 0.17178375611644014 1102 42-43 0.16225007638195166 1102 44-45 0.1169345987748116 1102 46-47 0.08065849629999633 1102 48-49 0.04806602984012187 1102 50-51 -0.03851211807707955 1102 52-53 -0.017403710389068294 1102 54-55 0.10972029877512313 1102 56-57 0.009454373326121868 1102 58-59 -0.016999585840530074 1102 60-61 0.03336644644485176 1102 62-63 0.05422226909787753 1102 64-65 0.13891587766276814 1102 66-67 0.22310137667358987 1102 68-69 0.27586833248599163 1102 70-71 0.1548528173454926 1102 72-73 0.1934396665323348 1102 74-75 0.03784486050800595 1102 76-77 -0.02794807197847149 1102 78-79 0.1291726738257637 1102 80-81 -0.061149212721645085 1102 82-83 -0.0035499434457300083 1102 84-85 0.1008170633754375 1102 86-87 0.11311312443367783 1102 88-89 0.17694717523486503 1102 90-91 0.12504204281649578 1102 92-93 -0.1843482916031718 1102 94-95 -0.3329462634314808 1102 96-97 0.10062809885856083 1102 98-99 0.3156286756643816 1102 100-101 0.48337181133363316 1103 1 -0.05660088583258016 1103 2 -0.0871300849526584 1103 3 -0.07380351826944676 1103 4 -0.11952189029938154 1103 5 -0.16976831994563923 1103 6 -0.11807784005665667 1103 7 0.014798516869703349 1103 8 0.08191158101627138 1103 9 0.02605311343162242 1103 10-11 -0.008139596845744279 1103 12-13 0.06167512022246768 1103 14-15 0.08662304454963987 1103 16-17 -6.631624063402342E-5 1103 18-19 -0.011610316020806977 1103 20-21 0.11750678929392677 1103 22-23 0.1559999272907433 1103 24-25 0.17622442982490583 1103 26-27 0.1997274217406826 1103 28-29 -0.10636859146885769 1103 30-31 -0.17127500560100373 1103 32-33 -0.23189142741982494 1103 34-35 0.08258992078074101 1103 36-37 0.16655548169297418 1103 38-39 0.16468700850896312 1103 40-41 0.1809483063790509 1103 42-43 0.07037803693916089 1103 44-45 -0.03521394255195531 1103 46-47 -0.040025985694171595 1103 48-49 -0.06485488842920262 1103 50-51 -0.14168259772368685 1103 52-53 -0.08708750441966373 1103 54-55 -0.04021432964936622 1103 56-57 -0.011900229965483788 1103 58-59 0.09123355729766303 1103 60-61 -0.06852249638667018 1103 62-63 -0.021888922388882293 1103 64-65 0.010867111436397181 1103 66-67 0.18897851687014366 1103 68-69 -0.006555584723152208 1103 70-71 0.07595569078792153 1103 72-73 0.14012315751146076 1103 74-75 0.12076869568944204 1103 76-77 0.10092013605181904 1103 78-79 0.2706982088352241 1103 80-81 0.2625931153484373 1103 82-83 0.21917216704877163 1103 84-85 0.19641409647821106 1103 86-87 0.3370469395976805 1103 88-89 0.4325934717421589 1103 90-91 0.3674245752909542 1103 92-93 0.17119957165011712 1103 94-95 0.23992642053863378 1103 96-97 0.31841584989927796 1103 98-99 0.38131382757936905 1103 100-101 0.9297475730970923 1104 1 0.05251800266839268 1104 2 0.027781274096287945 1104 3 0.2064546838219492 1104 4 0.06221840078076468 1104 5 0.17682699752383257 1104 6 0.13522193920387338 1104 7 0.10659449322155012 1104 8 0.21228923609128003 1104 9 0.24937982204858145 1104 10-11 0.24732678505831274 1104 12-13 0.2891586539432822 1104 14-15 0.3805938149842305 1104 16-17 0.372247973427811 1104 18-19 0.39094326606584673 1104 20-21 0.26328038650170527 1104 22-23 0.4044532151131506 1104 24-25 0.4001881219311727 1104 26-27 0.5005758632698374 1104 28-29 0.36774662574795514 1104 30-31 0.2372008549212481 1104 32-33 0.21971417724216025 1104 34-35 0.368603434673652 1104 36-37 0.3885893962331508 1104 38-39 0.24346173435172602 1104 40-41 0.3001603361681404 1104 42-43 0.35655604021638965 1104 44-45 0.5565960715485474 1104 46-47 0.6206390204444574 1104 48-49 0.6793540447976838 1104 50-51 0.5284704401607314 1104 52-53 0.46158500349185516 1104 54-55 0.5771182933188328 1104 56-57 0.6160036434952829 1104 58-59 0.47034641121820897 1104 60-61 0.5831211918616859 1104 62-63 0.665840863170466 1104 64-65 0.5493174959220823 1104 66-67 0.6182837540073507 1104 68-69 0.6175194515024955 1104 70-71 0.4740119085031651 1104 72-73 0.5396569721358873 1104 74-75 0.5752556721268292 1104 76-77 0.609294066003784 1104 78-79 0.716873021414763 1104 80-81 0.6436694374318641 1104 82-83 0.6363611755906611 1104 84-85 0.5643157437171844 1104 86-87 0.4818925590438923 1104 88-89 0.49622790762779445 1104 90-91 0.5714242766997799 1104 92-93 0.5556144006653838 1104 94-95 0.6532936932947351 1104 96-97 0.7604757854709661 1104 98-99 0.8980220514870751 1104 100-101 0.9195847385156135 1105 1 0.3017376125316602 1105 2 0.29293183839198633 1105 3 0.22551426995442725 1105 4 0.2928564344627915 1105 5 0.2950146044087276 1105 6 0.26590356654490677 1105 7 0.0821283034098812 1105 8 0.08677556101147132 1105 9 0.2718264905278218 1105 10-11 0.27011092432042716 1105 12-13 0.44770857964314104 1105 14-15 0.5690799977742387 1105 16-17 0.49802027593315756 1105 18-19 0.4313541990585321 1105 20-21 0.3914864031337615 1105 22-23 0.6089488421352769 1105 24-25 0.6264070384678675 1105 26-27 0.4063640617274018 1105 28-29 0.4937003033309466 1105 30-31 0.4879398788121918 1105 32-33 0.403118322281415 1105 34-35 0.5542708790493123 1105 36-37 0.6597686489041834 1105 38-39 0.584186838677752 1105 40-41 0.4782204572333555 1105 42-43 0.5756882470914633 1105 44-45 0.5802586248075912 1105 46-47 0.6208881018132786 1105 48-49 0.6067165082177226 1105 50-51 0.5447491185480686 1105 52-53 0.5905124545214662 1105 54-55 0.5936985563869399 1105 56-57 0.6204089718920045 1105 58-59 0.7703848075796742 1105 60-61 0.724116096035182 1105 62-63 0.7751688455265295 1105 64-65 0.7342544901799499 1105 66-67 0.7845148965678064 1105 68-69 0.7937384210728524 1105 70-71 0.7466483490543894 1105 72-73 0.9233069811692829 1105 74-75 0.734431571657062 1105 76-77 0.7361563867633123 1105 78-79 0.8492174304130913 1105 80-81 0.9568753815846058 1105 82-83 0.933699275352609 1105 84-85 0.8808949814680886 1105 86-87 0.8951443596246449 1105 88-89 1.01287640732356 1105 90-91 1.100717942749725 1105 92-93 0.870957424071257 1105 94-95 1.052050337085042 1105 96-97 1.3207200655268139 1105 98-99 1.3134775769391602 1105 100-101 1.4375129401481281 1106 1 -0.004312554167089644 1106 2 -0.08244209708030326 1106 3 -0.08801716503266732 1106 4 -0.10122303373886865 1106 5 -0.11050997602966817 1106 6 -0.033784530231301346 1106 7 -0.03893368710980383 1106 8 -0.0342789498145919 1106 9 -0.2168171693886194 1106 10-11 -0.27637970185195826 1106 12-13 -0.42211128965117695 1106 14-15 -0.5453526221931568 1106 16-17 -0.4072875902244135 1106 18-19 -0.3115657788488164 1106 20-21 -0.16057305176978787 1106 22-23 -0.33730129220395355 1106 24-25 -0.296432835857523 1106 26-27 -0.18133810159682895 1106 28-29 0.07027701290350308 1106 30-31 -0.07410673857510375 1106 32-33 0.029803074059898904 1106 34-35 -0.028270492513918555 1106 36-37 -0.1632948682603299 1106 38-39 -0.20509306759774404 1106 40-41 0.04682282924103731 1106 42-43 -0.07007936827592687 1106 44-45 -0.30186711199143446 1106 46-47 -0.09683239463367954 1106 48-49 0.03280453791289517 1106 50-51 0.022834994091482486 1106 52-53 0.18510302199794637 1106 54-55 0.18473626939582033 1106 56-57 0.050264798829218194 1106 58-59 2.3539873135547396E-4 1106 60-61 0.09472389200173836 1106 62-63 0.22102806745729708 1106 64-65 0.12134044485064521 1106 66-67 0.20985692524180166 1106 68-69 0.023331946964994188 1106 70-71 0.1435014057492836 1106 72-73 0.3542434269999646 1106 74-75 0.4418487901479651 1106 76-77 0.32812591212951503 1106 78-79 0.3810663594981136 1106 80-81 0.42587205154627483 1106 82-83 0.3145473623815995 1106 84-85 0.4283286395382291 1106 86-87 0.449929178681014 1106 88-89 0.4716640828355523 1106 90-91 0.5801375657652912 1106 92-93 0.6579097677938819 1106 94-95 0.7265862319378691 1106 96-97 0.8386421987669266 1106 98-99 0.9060775617435723 1106 100-101 1.1177532823874152 1107 1 0.07828299344814837 1107 2 -0.1409707311474122 1107 3 -0.009728421162016332 1107 4 0.043717949159393754 1107 5 0.07826877420679779 1107 6 0.0903568621663311 1107 7 -0.039921750157056124 1107 8 0.11031860922840764 1107 9 0.03590403298626654 1107 10-11 0.003833251635406043 1107 12-13 0.10245138213269911 1107 14-15 0.05007796033428491 1107 16-17 0.07350465599399314 1107 18-19 0.06300911421973865 1107 20-21 0.022125945087218213 1107 22-23 -0.048715319783006805 1107 24-25 -0.0256784276574038 1107 26-27 0.05511056574594164 1107 28-29 -0.039331027282081266 1107 30-31 -0.005657375942917042 1107 32-33 -0.047095666553019555 1107 34-35 0.16847089474195087 1107 36-37 0.03542475551595459 1107 38-39 0.10630564980048973 1107 40-41 0.18761529033916702 1107 42-43 0.18173032195576155 1107 44-45 0.10212884519991405 1107 46-47 -0.008379263931928449 1107 48-49 0.025857399477779097 1107 50-51 0.0272548015515639 1107 52-53 -0.04727597673157646 1107 54-55 0.022847223312176368 1107 56-57 -0.06244038733145629 1107 58-59 -0.14177285955715035 1107 60-61 -0.05284130960725264 1107 62-63 0.013227472777650462 1107 64-65 -0.1145497270493081 1107 66-67 0.07468721438166881 1107 68-69 -0.09448258036291435 1107 70-71 -0.050134585684688204 1107 72-73 -0.026685191104647288 1107 74-75 0.07048961062017156 1107 76-77 0.17908705765335498 1107 78-79 0.315565645598582 1107 80-81 0.48972375536986235 1107 82-83 0.33043464875373374 1107 84-85 0.32726724878289204 1107 86-87 0.35793532884587975 1107 88-89 0.37147736769815864 1107 90-91 0.47661445909554345 1107 92-93 0.6333465204393036 1107 94-95 0.3617123912811664 1107 96-97 0.5424174437777829 1107 98-99 0.604818289779125 1107 100-101 0.8643103143457864 1108 1 0.03723944079245811 1108 2 0.0015938511325686022 1108 3 0.026235963007316343 1108 4 -0.04447676412435442 1108 5 0.03861392483563719 1108 6 -1.5855615986737348E-4 1108 7 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-0.8128246838246973 2316 82-83 -0.8637751006474801 2316 84-85 -1.228813451617551 2316 86-87 -1.3039685702566892 2316 88-89 -1.302831413989587 2316 90-91 -1.227962455405958 2316 92-93 -1.5066079892474917 2316 94-95 -1.6582598508555435 2316 96-97 -1.8646253046892944 2316 98-99 -2.073758341713315 2316 100-101 -2.4821844199624827 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5556.0 3 1045.0 4 866.0 5 1081.0 6 1201.0 7 1378.0 8 1665.0 9 2121.0 10 2672.0 11 3586.0 12 4621.0 13 5946.0 14 6868.0 15 8554.0 16 10898.0 17 13908.0 18 11888.0 19 8454.0 20 8402.0 21 8875.0 22 9900.0 23 10990.0 24 12295.0 25 14143.0 26 16407.0 27 19296.0 28 23581.0 29 28324.0 30 35080.0 31 44004.0 32 55874.0 33 73671.0 34 100497.0 35 140639.0 36 207911.0 37 300376.0 38 261474.0 39 37234.0 40 28.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.327485348861444 19.606470284845393 14.783553311972378 22.282491054320783 2 14.780757792009597 20.575767513028705 40.954843845903646 23.688630849058054 3 19.51119490957714 25.125113291563228 29.711609939068005 25.652081859791632 4 12.015019776573476 16.665687077301623 38.66704158223274 32.65225156389216 5 13.049871879376285 38.295211020043176 34.49573498898437 14.15918211159617 6 30.824673461078962 37.154689679568044 17.90074017713527 14.119896682217728 7 28.00485125509872 33.13331139544337 20.874147042156125 17.98769030730178 8 25.916994279840612 35.14620780798419 19.896075984007584 19.040721928167613 9 25.946967674416122 17.436272572911438 20.85691630802747 35.75984344464497 10-11 24.39919350666732 27.355223199132496 27.552507675604375 20.693075618595806 12-13 25.58027610278743 24.594127967964788 28.18290349485666 21.64269243439113 14-15 22.373235650693125 26.065300404485953 26.892425957980315 24.669037986840607 16-17 21.26462157369834 29.549887980114427 27.367994919139765 21.81749552704747 18-19 21.19359992738718 28.26116989397727 29.74626224173395 20.798967936901594 20-21 22.320596676233066 26.99512781151972 29.60889007541881 21.075385436828405 22-23 21.67528864321836 27.017632615031808 29.688354091261914 21.61872465048792 24-25 21.580884855587307 27.26389369026184 29.78562666926497 21.369594784885887 26-27 21.470954349922742 27.18987882489179 29.966894600015355 21.372272225170118 28-29 21.05954655793704 27.822599753182498 29.821467783241108 21.296385905639358 30-31 21.4355322413884 27.229984587997595 29.76135091641008 21.573132254203927 32-33 21.346630520140852 27.321854152573007 29.700235922495672 21.631279404790472 34-35 21.88269929183618 27.34237840395672 29.4892283747405 21.285693929466603 36-37 21.69627339639113 27.069601176794087 29.837256425247677 21.3968690015671 38-39 21.291765604082162 27.64711810157619 29.74822846968344 21.31288782465821 40-41 21.535630327531063 27.56936948059857 29.535223228661096 21.359776963209274 42-43 21.303233056915843 27.56894087121561 29.196480648773605 21.931345423094943 44-45 21.53626972573692 27.441492745120417 29.226161751877044 21.79607577726562 46-47 21.806734761093878 27.858749613327365 28.842591619306567 21.49192400627219 48-49 21.675223715232992 27.8302584134114 28.793764839957888 21.70075303139772 50-51 21.531000197572528 28.000899755971233 28.91043423237733 21.55766581407891 52-53 21.805685723211496 28.294576748184998 28.224653353519336 21.675084175084177 54-55 21.425791731971263 27.949386826583932 28.579081080936753 22.045740360508052 56-57 21.75917367979558 28.13088297022554 28.32774663179622 21.78219671818266 58-59 22.08406217669131 27.826641653285307 28.421237708639925 21.668058461383453 60-61 21.75001201165932 28.09130062247088 28.863750413734934 21.294936952134872 62-63 21.50826161566045 28.12459439322561 28.831522165273686 21.535621825840252 64-65 21.413864585220544 28.17856448148856 28.637801215622723 21.76976971766817 66-67 21.563781696304776 28.086323867669126 28.23898869479415 22.11090574123195 68-69 21.51546919407244 28.04687703320323 28.501680614510338 21.935973158213994 70-71 21.782937743729477 28.01480453759344 28.360893304089966 21.841364414587115 72-73 21.70939828673067 27.743917293207993 28.64163171049953 21.905052709561804 74-75 21.327404512550693 28.488304193085757 28.304146370599348 21.8801449237642 76-77 21.5380564850792 28.02804098832204 28.27901237204497 22.15489015455379 78-79 21.62623107052832 27.98822203283389 28.314438621160964 22.071108275476828 80-81 21.703554003387943 28.22529385916181 28.13607425046687 21.93507788698338 82-83 21.79816908087299 28.112447386853994 28.360492111657166 21.72889142061584 84-85 21.59279533933833 28.21974455244629 28.222280276967847 21.965179831247532 86-87 21.522524530477583 28.279650113710375 28.20848103225531 21.989344323556733 88-89 21.623889756832636 27.953635564619837 28.095116698577783 22.32735797996974 90-91 21.633825957868375 27.723602166342282 28.331355205190896 22.311216670598455 92-93 21.893972712996597 27.788915814462257 28.424573653085456 21.892537819455686 94-95 21.534100483681787 27.723959350041127 28.519273025772446 22.222667140504637 96-97 21.17836424533584 27.815897353421853 28.77825907356752 22.22747932767479 98-99 21.36989738800056 27.926907928829962 28.204201582809528 22.49899310035995 100-101 21.300029727815325 27.596854790465613 28.608134344368214 22.494981137350848 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1964.0 1 1099.0 2 251.5 3 267.0 4 236.5 5 236.5 6 367.0 7 730.0 8 1226.0 9 1484.5 10 1515.0 11 1510.0 12 1535.5 13 1606.5 14 1772.0 15 1876.0 16 1900.0 17 2026.5 18 2185.5 19 2405.0 20 2692.0 21 3114.5 22 3657.5 23 4531.0 24 5594.5 25 6979.0 26 8883.0 27 11199.0 28 13364.5 29 15922.5 30 19177.5 31 22487.0 32 26352.5 33 30419.5 34 35131.5 35 38892.5 36 42810.0 37 47082.5 38 48939.5 39 49941.5 40 51160.5 41 52694.5 42 53583.0 43 54779.0 44 56795.0 45 57761.5 46 57688.5 47 60363.0 48 66924.0 49 65070.5 50 56044.0 51 50537.5 52 46826.0 53 44302.5 54 41693.0 55 37676.5 56 33186.0 57 28861.0 58 25190.0 59 21645.0 60 17784.5 61 14260.5 62 11270.5 63 8498.5 64 6248.5 65 4738.5 66 3571.5 67 2738.5 68 2027.0 69 1405.5 70 990.5 71 703.5 72 506.5 73 384.0 74 303.0 75 233.0 76 163.5 77 105.5 78 63.0 79 35.0 80 27.0 81 24.5 82 15.5 83 9.5 84 5.5 85 4.0 86 4.0 87 3.5 88 3.0 89 3.5 90 3.0 91 2.5 92 1.5 93 2.0 94 2.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29953860264609083 2 0.26896528296306754 3 0.2708303220722716 4 0.27196266724571694 5 0.2592404361793608 6 0.2010911810959636 7 0.2091508143893096 8 0.206419864265118 9 0.2033558714428542 10-11 0.21437958474904234 12-13 0.2136468908132836 14-15 0.19899301209810905 16-17 0.2089842930402735 18-19 0.19656180040218238 20-21 0.20042509569981928 22-23 0.200225270080976 24-25 0.19283172218377428 26-27 0.20408856537861295 28-29 0.2022235262694089 30-31 0.20821829483470758 32-33 0.20965037843641782 34-35 0.19965909749425334 36-37 0.19989222738290385 38-39 0.19229885386685885 40-41 0.19326467769126807 42-43 0.18990094644073938 44-45 0.2048878678539861 46-47 0.1963952790531463 48-49 0.20192378784114395 50-51 0.20815168629509315 52-53 0.215878276890367 54-55 0.2132139353057898 56-57 0.18716999631654777 58-59 0.17614628301035962 60-61 0.18417261203389843 62-63 0.18533826147715093 64-65 0.18220766011527273 66-67 0.19506310826085768 68-69 0.18317348393968197 70-71 0.18980103363131773 72-73 0.20009205300174715 74-75 0.19090007453495583 76-77 0.19522962960989376 78-79 0.19436371859490617 80-81 0.18380626506601905 82-83 0.19919283771695234 84-85 0.18140835763989957 86-87 0.18417261203389843 88-89 0.19156615993110013 90-91 0.189068339695559 92-93 0.19582910646642362 94-95 0.19359772038934023 96-97 0.1870367792373189 98-99 0.19299824353281037 100-101 0.18067566370414087 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1501309.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.8327475101445 #Duplication Level Percentage of deduplicated Percentage of total 1 79.93188104186049 49.42407818472267 2 11.965890473529706 14.797677687676114 3 3.6311572179584672 6.73573282282994 4 1.513854636674915 3.744231660663263 5 0.8146891380989498 2.51872338876648 6 0.4790719017063895 1.7773399162449566 7 0.31590220114492423 1.3673170728905086 8 0.21834891996348246 1.080089090977181 9 0.16038620153224192 0.892540755310884 >10 0.8830454520360002 9.972145556008151 >50 0.05780056324610972 2.4530439997227886 >100 0.025605477927121246 2.6582297019323122 >500 9.682258587099831E-4 0.4133557291645406 >1k 0.001290967811613311 1.7806092948626364 >5k 1.0758065096777591E-4 0.3848851382275558 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5786 0.38539701020909084 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4663 0.3105956202220862 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4136 0.2754929198452817 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2881 0.19189920262917226 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2759 0.18377296079621183 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2175 0.14487357366138484 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2043 0.1360812464322801 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1799 0.11982876276635923 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.660853961442981E-5 0.0 3 0.0 0.0 0.0 6.660853961442981E-5 0.0 4 0.0 0.0 0.0 6.660853961442981E-5 0.0 5 0.0 0.0 0.0 6.660853961442981E-5 0.0 6 0.0 0.0 0.0 6.660853961442981E-5 0.0 7 1.3321707922885962E-4 0.0 0.0 1.3321707922885962E-4 0.0 8 1.3321707922885962E-4 0.0 0.0 1.9982561884328943E-4 0.0 9 1.3321707922885962E-4 0.0 0.0 1.9982561884328943E-4 0.0 10-11 1.3321707922885962E-4 0.0 0.0 2.6643415845771924E-4 0.0 12-13 1.9982561884328943E-4 0.0 0.0 2.9973842826493417E-4 0.0 14-15 1.9982561884328943E-4 0.0 0.0 4.329555074937938E-4 0.0 16-17 1.9982561884328943E-4 0.0 0.0 4.995640471082235E-4 0.0 18-19 2.3312988865050434E-4 0.0 0.0 5.994768565298682E-4 0.0 20-21 2.6643415845771924E-4 0.0 0.0 5.994768565298682E-4 0.0 22-23 2.6643415845771924E-4 0.0 0.0 6.660853961442981E-4 0.0 24-25 2.6643415845771924E-4 0.0 0.0 6.99389665951513E-4 0.0 26-27 2.6643415845771924E-4 0.0 0.0 8.992152847948024E-4 0.0 28-29 4.329555074937938E-4 0.0 0.0 0.0015653006809391006 0.0 30-31 5.328683169154385E-4 0.0 0.0 0.002797558663806052 0.0 32-33 5.328683169154385E-4 0.0 0.0 0.0052953788993471695 0.0 34-35 5.994768565298682E-4 0.0 0.0 0.006960592389707915 0.0 36-37 5.994768565298682E-4 0.0 0.0 0.009391804085634604 0.0 38-39 5.994768565298682E-4 0.0 0.0 0.01188962432117572 0.0 40-41 5.994768565298682E-4 0.0 0.0 0.01482040006421063 0.0 42-43 5.994768565298682E-4 0.0 0.0 0.020015866154136155 0.0 44-45 5.994768565298682E-4 0.0 0.0 0.029707408668035694 0.0 46-47 6.660853961442981E-4 0.0 0.0 0.04978988336178628 0.0 48-49 7.326939357587279E-4 0.0 0.0 0.09305212984135844 0.0 50-51 7.326939357587279E-4 0.0 0.0 0.12712239785413929 0.0 52-53 7.326939357587279E-4 0.0 0.0 0.16235831531017264 0.0 54-55 7.326939357587279E-4 0.0 0.0 0.2100833339439116 0.0 56-57 7.326939357587279E-4 0.0 0.0 0.24528594713013777 0.0 58-59 7.326939357587279E-4 0.0 0.0 0.2838855958366998 0.0 60-61 7.326939357587279E-4 0.0 0.0 0.3564889040164283 0.0 62-63 7.326939357587279E-4 0.0 0.0 0.4059790489499497 0.0 64-65 7.326939357587279E-4 0.0 0.0 0.4585997952453492 0.0 66-67 7.326939357587279E-4 0.0 0.0 0.5713014442729645 0.0 68-69 7.326939357587279E-4 0.0 0.0 0.6955596749236832 0.0 70-71 7.326939357587279E-4 0.0 0.0 0.8127574003752726 0.0 72-73 7.326939357587279E-4 0.0 0.0 0.9468736948889269 0.0 74-75 7.326939357587279E-4 0.0 0.0 1.0689338437323697 0.0 76-77 7.326939357587279E-4 0.0 0.0 1.1682471762974842 0.0 78-79 7.326939357587279E-4 0.0 0.0 1.3050944209353306 0.0 80-81 8.659110149875875E-4 0.0 0.0 1.526168163915623 0.0 82-83 8.659110149875875E-4 0.0 0.0 1.710740427187208 0.0 84-85 8.659110149875875E-4 0.0 0.0 1.9139630815508333 0.0 86-87 9.325195546020173E-4 0.0 0.0 2.2657560835244444 0.0 88-89 9.325195546020173E-4 0.0 0.0 2.7513323373136376 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7340 0.0 26.493677 1 TACATGG 7285 0.0 25.707836 2 ACATGGG 7430 0.0 24.565565 3 GTATCAA 11380 0.0 22.686764 1 GAGTACT 5045 0.0 22.407982 12-13 AGTACTT 5200 0.0 21.237658 12-13 CATGGGA 4840 0.0 20.920021 4 GTACTTT 5330 0.0 20.762836 14-15 GGACCGT 185 6.5485237E-6 20.542627 6 AGAGTAC 8815 0.0 20.207062 10-11 CAACGCA 12875 0.0 19.971922 5 TCAACGC 12900 0.0 19.89903 4 ATCAACG 12980 0.0 19.774405 3 CATGGGG 2795 0.0 19.728952 4 AACGCAG 13115 0.0 19.668411 6 TATCAAC 13290 0.0 19.313795 2 TAGCCCT 895 0.0 19.120857 4 GTGTACG 155 9.5638505E-4 18.404945 1 GAGTACA 5700 0.0 18.267715 1 GGTATCA 8710 0.0 18.123201 1 >>END_MODULE