FastQCFastQC Report
Thu 26 May 2016
SRR937205_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937205_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501309
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72830.4851099940118923No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57240.3812672807529962No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT52800.3516930891641894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39530.263303557095841No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT23530.15672989371275334No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT21500.1432083601710241No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT17010.1133011258841451No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC16380.10910478788843601No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15350.10224410830814977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG67200.024.1169261
TACATGG67400.023.1883722
GTATCAA129400.022.8450931
GAGTACT65200.022.83919712-13
ACATGGG67450.022.3964633
GTACTTT69250.021.4341714-15
AGTACTT67400.020.8956412-13
AGAGTAC105450.020.2476210-11
TCAACGC148050.019.9579644
CAACGCA148250.019.931045
ATCAACG148300.019.924323
TATCAAC150150.019.7421072
CATGGGA42350.019.7421074
AACGCAG151000.019.6912736
GGTATCA99500.018.6762851
AACGCGA650.00756762518.26850776-77
TACTTTT78250.017.93698914-15
ACGCAGA165100.017.9238727
CGCAGAG165650.017.7783368
GTGTAGA8300.017.7509021
Produced by FastQC (version 0.11.5)