##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937205_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1501309 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1928796803323 33.0 31.0 34.0 31.0 34.0 2 32.41189921595088 34.0 31.0 34.0 31.0 34.0 3 32.49750517714874 34.0 31.0 34.0 31.0 34.0 4 36.096906766028845 37.0 35.0 37.0 35.0 37.0 5 35.99408116516986 37.0 35.0 37.0 35.0 37.0 6 36.078807893644814 37.0 35.0 37.0 35.0 37.0 7 36.03559560356995 37.0 35.0 37.0 35.0 37.0 8 36.02024499953041 37.0 35.0 37.0 35.0 37.0 9 37.71936356872569 39.0 37.0 39.0 35.0 39.0 10-11 37.64661305567341 39.0 37.0 39.0 35.0 39.0 12-13 37.57631540209244 39.0 37.0 39.0 35.0 39.0 14-15 38.79579253837818 40.0 38.0 41.0 35.0 41.0 16-17 38.78284017480745 40.0 38.0 41.0 35.0 41.0 18-19 38.79368104767239 40.0 38.0 41.0 35.0 41.0 20-21 38.69382285725324 40.0 38.0 41.0 34.0 41.0 22-23 38.573968450199125 40.0 38.0 41.0 34.0 41.0 24-25 38.437438595252544 40.0 38.0 41.0 34.0 41.0 26-27 38.39187935328437 40.0 38.0 41.0 34.0 41.0 28-29 38.31564055101248 40.0 38.0 41.0 34.0 41.0 30-31 38.39880564227617 40.0 38.0 41.0 34.0 41.0 32-33 38.33278026042606 40.0 38.0 41.0 34.0 41.0 34-35 38.22286284835434 40.0 38.0 41.0 34.0 41.0 36-37 38.073309358699646 40.0 38.0 41.0 33.5 41.0 38-39 37.95241852276913 40.0 37.0 41.0 33.0 41.0 40-41 37.81483658593933 40.0 37.0 41.0 33.0 41.0 42-43 37.61723535927648 40.0 37.0 41.0 33.0 41.0 44-45 37.329727591055544 40.0 37.0 41.0 32.5 41.0 46-47 36.997306017615294 40.0 37.0 41.0 31.5 41.0 48-49 36.70655940915561 40.0 36.0 41.0 31.0 41.0 50-51 36.50013121882304 39.5 36.0 41.0 30.5 41.0 52-53 36.294179612591414 39.0 36.0 41.0 30.0 41.0 54-55 36.091014907657254 39.0 35.5 40.5 30.0 41.0 56-57 35.855231667831205 39.0 35.0 40.0 29.0 41.0 58-59 35.63101067135413 39.0 35.0 40.0 28.0 41.0 60-61 35.31572148038811 38.0 35.0 40.0 28.0 41.0 62-63 34.97335758328232 38.0 34.0 40.0 27.5 41.0 64-65 34.64156912401111 37.5 34.0 40.0 27.0 41.0 66-67 34.218026069250236 37.0 34.0 39.5 26.0 41.0 68-69 33.8185576720049 36.0 33.0 39.0 26.0 40.5 70-71 33.41191187157341 36.0 33.0 39.0 25.5 40.0 72-73 32.966617465158734 35.0 32.5 38.0 25.0 40.0 74-75 32.522249250487405 35.0 32.0 37.5 24.0 39.5 76-77 31.17517513050278 34.0 30.5 36.0 22.5 38.5 78-79 31.819820902958682 35.0 31.5 36.0 24.0 39.0 80-81 31.663781406759036 35.0 32.0 36.0 23.0 38.0 82-83 31.324307987229812 34.5 31.5 36.0 21.5 37.0 84-85 30.949451778414705 34.0 31.0 35.0 20.0 37.0 86-87 30.598420112048885 34.0 31.0 35.0 19.5 36.0 88-89 30.2066643176055 34.0 31.0 35.0 17.5 36.0 90-91 29.75898332721645 34.0 30.0 35.0 11.5 35.5 92-93 29.406422328781083 34.0 30.0 35.0 6.0 35.0 94-95 29.001322179511348 34.0 29.0 35.0 2.0 35.0 96-97 28.54239533633649 34.0 29.0 35.0 2.0 35.0 98-99 28.05891225590468 34.0 29.0 35.0 2.0 35.0 100-101 26.11374607092877 31.5 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17844107800081588 1101 2 0.1435937076603082 1101 3 0.0665433233085082 1101 4 0.06218124543966752 1101 5 0.041395261306746534 1101 6 -0.004409056849674187 1101 7 0.035517838022691706 1101 8 0.1069138852693925 1101 9 0.044768081010246874 1101 10-11 0.12203225673081164 1101 12-13 0.07611975728490705 1101 14-15 0.06087025926644429 1101 16-17 0.06161076873235771 1101 18-19 0.025385295541397568 1101 20-21 0.05747469738707167 1101 22-23 0.022498233990205563 1101 24-25 0.13355009883809998 1101 26-27 -0.01365439586763273 1101 28-29 0.042443813477738956 1101 30-31 0.05428625310322133 1101 32-33 0.06872200277523177 1101 34-35 -0.03128646144806879 1101 36-37 -0.13968745610350197 1101 38-39 -0.08200645984094734 1101 40-41 -0.19430537632277378 1101 42-43 -0.059286340543920346 1101 44-45 -0.20843044012065803 1101 46-47 -0.15613847426010352 1101 48-49 -0.2029382431547191 1101 50-51 -0.30588554589091643 1101 52-53 -0.3026977632722847 1101 54-55 -0.5491752617581867 1101 56-57 -0.4865426360585303 1101 58-59 -0.5316350119125133 1101 60-61 -0.4506154618872742 1101 62-63 -0.5186997789502712 1101 64-65 -0.5106494464986113 1101 66-67 -0.3508183045882376 1101 68-69 -0.3868511897762872 1101 70-71 -0.7383460884525661 1101 72-73 -0.6080792373816308 1101 74-75 -0.4692025559366897 1101 76-77 -0.5519688901760453 1101 78-79 -0.6784760334150342 1101 80-81 -0.6182874445667288 1101 82-83 -0.5931541207597917 1101 84-85 -0.4047195465239284 1101 86-87 -0.4352545062519333 1101 88-89 -0.5477120520578822 1101 90-91 -0.6124135004874667 1101 92-93 -0.7025293806056148 1101 94-95 -0.5401167526067141 1101 96-97 -0.5313810777950394 1101 98-99 -0.6469773742297917 1101 100-101 -0.11575071686093352 1102 1 0.0042059882498080015 1102 2 0.045895635032302096 1102 3 0.10794393543523739 1102 4 0.025958563662840106 1102 5 -0.11467885565909341 1102 6 -0.048150309227871446 1102 7 0.015509566039902722 1102 8 -0.006838451565748471 1102 9 0.04968594022109585 1102 10-11 0.024921329441539797 1102 12-13 -0.015587579963828091 1102 14-15 0.04687290630094054 1102 16-17 -0.029048011977373278 1102 18-19 -0.05175706528248725 1102 20-21 0.024766781099536672 1102 22-23 0.012965601018080974 1102 24-25 0.028400293229694284 1102 26-27 0.028042187803464458 1102 28-29 -0.013735853988556812 1102 30-31 -0.03784841477666845 1102 32-33 0.050301289730320775 1102 34-35 2.0381897213894717E-4 1102 36-37 0.07017357458555296 1102 38-39 0.024546604928673332 1102 40-41 0.19810243242898196 1102 42-43 0.08636061123041827 1102 44-45 0.040760725401717934 1102 46-47 0.048612125458653566 1102 48-49 0.13698482740529272 1102 50-51 0.15857354915416977 1102 52-53 -0.04950080736195872 1102 54-55 -0.00858546938495408 1102 56-57 -0.10304855052622486 1102 58-59 0.08661000421786014 1102 60-61 -0.04269900891349465 1102 62-63 -0.19135475454792328 1102 64-65 -0.04390711876265385 1102 66-67 -0.07670547474292277 1102 68-69 -0.1161222626524605 1102 70-71 -0.04187888686434604 1102 72-73 -0.08813780301981211 1102 74-75 -0.038115782837174095 1102 76-77 -0.09059425207529159 1102 78-79 -0.09767894515298181 1102 80-81 0.0028800630848557773 1102 82-83 0.010868213593752785 1102 84-85 -0.013137116215382605 1102 86-87 8.967055935507062E-4 1102 88-89 0.1050722973506737 1102 90-91 0.09123494024377621 1102 92-93 0.1494668142822988 1102 94-95 -0.04683178279945466 1102 96-97 -0.07787921659891239 1102 98-99 0.034419763664160996 1102 100-101 0.50616110380248 1103 1 0.10861072601004906 1103 2 0.0628511472778186 1103 3 0.08918515003342975 1103 4 0.07634462850784018 1103 5 -0.07381535611968815 1103 6 -0.0020557471472599786 1103 7 0.07537275517709219 1103 8 0.052606078823096425 1103 9 0.06492371533552443 1103 10-11 0.014287965246190026 1103 12-13 0.07687402321516856 1103 14-15 -0.018307549652853083 1103 16-17 0.12277258556526505 1103 18-19 0.04875962576973336 1103 20-21 0.06675060209402517 1103 22-23 0.14829495313949792 1103 24-25 0.06865652040269055 1103 26-27 0.11288339986231222 1103 28-29 -0.05744275811570532 1103 30-31 -0.028342054538356365 1103 32-33 0.0450181036737618 1103 34-35 0.06933129471382671 1103 36-37 0.0942825381261656 1103 38-39 0.13635806080862523 1103 40-41 0.153713913101015 1103 42-43 0.2104155801551144 1103 44-45 -0.08790550400657793 1103 46-47 0.06162345797962132 1103 48-49 0.09469344425768611 1103 50-51 0.07938640201115277 1103 52-53 0.16186756522494505 1103 54-55 0.14128801414651804 1103 56-57 -0.012283300472020642 1103 58-59 0.016486000370065312 1103 60-61 -0.052448946348569336 1103 62-63 -0.09319932556638122 1103 64-65 -0.27109030050899463 1103 66-67 -0.2334124847877277 1103 68-69 -0.09418628168956644 1103 70-71 -0.21241516767109658 1103 72-73 -0.13058455433819205 1103 74-75 -0.04306851917137777 1103 76-77 -0.12592965186098581 1103 78-79 -0.07699025318257213 1103 80-81 -0.06970727805119026 1103 82-83 -0.14043348767101094 1103 84-85 -0.12416889117811891 1103 86-87 -0.003408162174775242 1103 88-89 -0.030047391271466495 1103 90-91 -0.0717347435836686 1103 92-93 -0.07759508005331028 1103 94-95 0.13923680439600616 1103 96-97 0.11765329694735627 1103 98-99 0.08988603356841196 1103 100-101 0.45871701044926283 1104 1 0.1063494924255437 1104 2 0.03346270757853631 1104 3 0.14532425388337344 1104 4 0.05442460139681771 1104 5 0.04658129450651671 1104 6 -0.03978954585122807 1104 7 -0.06855281370680189 1104 8 0.06760797309341626 1104 9 0.03462595982290395 1104 10-11 0.04633399849219444 1104 12-13 0.06776666218309657 1104 14-15 0.016234230155312446 1104 16-17 0.10566882744525685 1104 18-19 -0.022763694564090997 1104 20-21 -0.11898830040505715 1104 22-23 -0.09125922778099493 1104 24-25 -0.009733571126730567 1104 26-27 -0.08170820239310927 1104 28-29 -0.15014887603081206 1104 30-31 -0.059744002845754096 1104 32-33 -0.011339163305400746 1104 34-35 -0.08394440567899863 1104 36-37 -0.15987635135249434 1104 38-39 -0.3495818952812968 1104 40-41 -0.1846972317484017 1104 42-43 -0.1639976006633077 1104 44-45 -0.09753077538928068 1104 46-47 -0.04030480020662708 1104 48-49 -0.05189318632260154 1104 50-51 -0.07064096334123349 1104 52-53 0.062407969003452024 1104 54-55 0.09651040346383155 1104 56-57 0.23123150737102094 1104 58-59 0.11613700542332595 1104 60-61 0.0467941382435626 1104 62-63 0.1279609455570423 1104 64-65 -0.06233576590330614 1104 66-67 0.1754921050936673 1104 68-69 0.13242773736411806 1104 70-71 0.008895059638035718 1104 72-73 -0.12984782025839792 1104 74-75 0.15729903863358885 1104 76-77 0.18027724605749995 1104 78-79 0.13568015269993694 1104 80-81 0.07119934912040904 1104 82-83 0.29639416609931146 1104 84-85 0.2487059175369133 1104 86-87 0.2633268986053743 1104 88-89 0.41558094001015533 1104 90-91 0.22121688629804126 1104 92-93 0.20499388376596173 1104 94-95 0.13950071407085574 1104 96-97 0.2877148417404918 1104 98-99 0.4040496925786172 1104 100-101 0.7189628328978337 1105 1 0.07968382043441835 1105 2 0.1836904163870372 1105 3 0.11607336830983428 1105 4 0.08541322285076802 1105 5 0.0776027968224966 1105 6 0.08410801718191152 1105 7 0.06407514853182761 1105 8 0.03991097341904748 1105 9 0.15078096374208627 1105 10-11 0.042646568916460126 1105 12-13 0.1139693999214515 1105 14-15 0.22367287110797207 1105 16-17 0.2693511728618958 1105 18-19 0.31512517024359 1105 20-21 0.21183082550849264 1105 22-23 0.21465565038480605 1105 24-25 0.18760180624126122 1105 26-27 0.18799477028430545 1105 28-29 0.108187041219729 1105 30-31 0.2181482490442832 1105 32-33 0.22621463491147153 1105 34-35 0.1797205093451808 1105 36-37 0.21436442777543618 1105 38-39 0.18986683054993847 1105 40-41 0.25317066709025937 1105 42-43 0.12788581157819578 1105 44-45 0.359237785415381 1105 46-47 0.33713868095034627 1105 48-49 0.28290045798354413 1105 50-51 0.2127204719331388 1105 52-53 0.1216387760376918 1105 54-55 0.3270208028319459 1105 56-57 0.25838986195611824 1105 58-59 0.2684804032467554 1105 60-61 0.30976519636738686 1105 62-63 0.2639507107700112 1105 64-65 0.2462424620869328 1105 66-67 0.43213854708387345 1105 68-69 0.3939018323714194 1105 70-71 0.28900977495518276 1105 72-73 0.3711481516592201 1105 74-75 0.4248216212449947 1105 76-77 0.21685013391289942 1105 78-79 0.30296153827825023 1105 80-81 0.23993350310820105 1105 82-83 0.4516991396302181 1105 84-85 0.36519839223897677 1105 86-87 0.4646311374434724 1105 88-89 0.5438762299504134 1105 90-91 0.47182562419266816 1105 92-93 0.38261612367392317 1105 94-95 0.4394415285192004 1105 96-97 0.37274386705627904 1105 98-99 0.42094550585491675 1105 100-101 0.7566064701151518 1106 1 -0.11747224258554212 1106 2 0.013958453176108776 1106 3 -0.04858696896135939 1106 4 0.04184873155456614 1106 5 -0.09651423518783986 1106 6 -0.055728004313941426 1106 7 -0.07577780069168227 1106 8 -0.016690230181758636 1106 9 -0.044675240486611756 1106 10-11 -0.15135188239329267 1106 12-13 -0.26641415876716934 1106 14-15 -0.13951743080001933 1106 16-17 -0.022207677957986505 1106 18-19 -0.049938204413081166 1106 20-21 -0.08345055072364715 1106 22-23 -0.2442121365824974 1106 24-25 -0.007580011332166237 1106 26-27 -0.014948213585931569 1106 28-29 -0.025573303885458643 1106 30-31 -0.027435680794617667 1106 32-33 -0.06871184954198384 1106 34-35 -0.1386394615391211 1106 36-37 0.03157449418330316 1106 38-39 0.06623536843911637 1106 40-41 -0.06701356170017903 1106 42-43 -0.4616516323585387 1106 44-45 -0.3718571905531505 1106 46-47 -0.14500217270314408 1106 48-49 -0.10733503820161161 1106 50-51 -0.19467166990074247 1106 52-53 -0.15079882855499704 1106 54-55 -0.19877646361560153 1106 56-57 -0.05033941289545396 1106 58-59 -0.09599739013339814 1106 60-61 -0.04953251551560811 1106 62-63 0.016916901373868143 1106 64-65 0.03798535273657677 1106 66-67 0.0578359793050538 1106 68-69 0.09225051285505259 1106 70-71 0.011469444644042426 1106 72-73 0.08346988398775324 1106 74-75 -0.027904549927406208 1106 76-77 0.17309038324651738 1106 78-79 0.13539376536489556 1106 80-81 0.018115906066068277 1106 82-83 0.043322433915612635 1106 84-85 0.0022712574458445545 1106 86-87 0.07554436473049364 1106 88-89 0.09282058929694159 1106 90-91 0.2006869274466112 1106 92-93 0.25683090346977266 1106 94-95 -0.3113510237530299 1106 96-97 0.15512060662633687 1106 98-99 0.3680249020153141 1106 100-101 0.5662691319963891 1107 1 -0.012812383769201574 1107 2 -0.0030043829907597797 1107 3 0.030640338875720374 1107 4 -0.012879217276427823 1107 5 -0.11225916373840761 1107 6 -0.04375574989173714 1107 7 -0.03456386835040348 1107 8 0.008699728565169096 1107 9 -0.10352158958406221 1107 10-11 -0.03666577371556201 1107 12-13 -0.03671674396219515 1107 14-15 -0.17526133099759278 1107 16-17 -0.057739192564618236 1107 18-19 -0.06960756590179074 1107 20-21 -0.10999395316767391 1107 22-23 0.08172933401601057 1107 24-25 -0.014588543976032042 1107 26-27 -0.06818178900429928 1107 28-29 -0.05968124027645416 1107 30-31 -0.08855265633170717 1107 32-33 -0.11784237324609848 1107 34-35 -0.1179641943112344 1107 36-37 -0.2098319473738144 1107 38-39 -0.12323291550449511 1107 40-41 -0.11786484996171964 1107 42-43 -0.20645087137732787 1107 44-45 -0.2778639999383259 1107 46-47 -0.2593559852259517 1107 48-49 -0.25121466871591736 1107 50-51 -0.1833622810762563 1107 52-53 -0.17702355668416914 1107 54-55 -0.2650804823653985 1107 56-57 -0.1950160169822368 1107 58-59 -0.30816790947852013 1107 60-61 -0.20232389914537663 1107 62-63 -0.32418855507480515 1107 64-65 -0.24150314908741422 1107 66-67 -0.052740554523730054 1107 68-69 -0.1902737407352575 1107 70-71 -0.35829482845428373 1107 72-73 -0.20333201109797727 1107 74-75 -0.293736485583203 1107 76-77 -0.2735570900556361 1107 78-79 -0.344451091469562 1107 80-81 -0.1285272312700947 1107 82-83 -0.0961370054247972 1107 84-85 -0.018496101095184514 1107 86-87 -0.03398367112679068 1107 88-89 -0.06393033708410911 1107 90-91 -0.18047422122368317 1107 92-93 -0.15156816963115816 1107 94-95 -0.29820172723473704 1107 96-97 -0.2909654933794563 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 11.0 5 18.0 6 42.0 7 109.0 8 316.0 9 594.0 10 986.0 11 1739.0 12 2759.0 13 3903.0 14 5094.0 15 7244.0 16 10088.0 17 14053.0 18 12544.0 19 7311.0 20 6010.0 21 5954.0 22 6297.0 23 6862.0 24 7891.0 25 9489.0 26 11778.0 27 14553.0 28 18558.0 29 23885.0 30 30236.0 31 38751.0 32 49878.0 33 66408.0 34 93089.0 35 134326.0 36 211847.0 37 345241.0 38 318782.0 39 34651.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.74307596544614 20.143578998439185 15.248337551932758 21.865007484181916 2 15.410951376432166 21.17811856186834 39.99256648697903 23.41836357472046 3 19.697410726239568 25.149852561997566 30.164210032711452 24.988526679051414 4 12.654023921790916 17.3156891752464 38.14338021020323 31.886906692759453 5 13.02321150435757 38.28958886551291 34.598811777763274 14.088387852366251 6 31.08107658050408 37.426206064174664 17.403878881695906 14.088838473625351 7 28.053185586711333 33.404116008096935 20.73930150288848 17.803396902303255 8 26.209860861421596 35.108495319750965 19.63759625766581 19.044047561161626 9 25.97286767747346 17.198125102826932 20.86545807691821 35.963549142781396 10-11 24.541949725206468 27.39659190746209 27.436390509881708 20.625067857449732 12-13 25.84011454313469 24.598597785805804 28.147929567562365 21.413358103497142 14-15 22.474620481193412 26.042573514179956 27.06028539094883 24.422520613677797 16-17 21.220048637555628 29.496825770044676 27.587358764917813 21.695766827481886 18-19 21.086764949787153 28.002796226493015 30.262157890214475 20.64828093350536 20-21 22.091821204029284 26.676120638722605 30.290932779327907 20.9411253779202 22-23 21.602775736022657 26.677293044573837 30.351652955730568 21.36827826367294 24-25 21.66332402366738 26.79238823238685 30.46508808658056 21.079199657365216 26-27 21.554789029837995 26.884537003331467 30.628149581976306 20.932524384854233 28-29 21.006277410381706 27.493412182865544 30.526106140458392 20.974204266294354 30-31 21.43203031487855 26.886403798285365 30.409962239618892 21.271603647217194 32-33 21.309770340416264 26.930998215557224 30.420586301687397 21.338645142339118 34-35 21.820291492291062 26.95134712440943 30.283372710081668 20.94498867321784 36-37 21.679680865165 26.6613002386584 30.524429014946293 21.134589881230312 38-39 21.35549710286157 27.245323913997716 30.454057092843644 20.945121890297067 40-41 21.523983403816267 27.190971345672345 30.177365219285303 21.107680031226085 42-43 21.326902611536994 27.161183843477655 29.88087287722062 21.63104066776473 44-45 21.543399793113878 27.058553568918857 29.816413543114706 21.58163309485256 46-47 21.909447022564976 27.546927381371855 29.358446528995696 21.185179067067473 48-49 21.783190535725826 27.551057110827955 29.2476765276169 21.418075825829327 50-51 21.599350966389995 27.758442798917475 29.289673211843798 21.35253302284873 52-53 21.895659054864787 28.121825686784003 28.535997586106525 21.44651767224469 54-55 21.55112638370915 27.655865647911256 28.900712644765335 21.89229532361426 56-57 21.882503868290936 27.828448374052243 28.68843122901415 21.60061652864267 58-59 22.20582171957938 27.55465397196713 28.760102017639273 21.479422290814217 60-61 21.860156703250297 27.842935731418383 29.216004167030242 21.080903398301082 62-63 21.612905804201535 27.795876798180785 29.24358010243061 21.347637295187067 64-65 21.529411999794846 27.93225778304133 28.94730531822563 21.591024898938194 66-67 21.690604665661766 27.77925796754699 28.57582949279595 21.954307873995294 68-69 21.683777290351287 27.74358909458346 28.766496437442257 21.806137177622993 70-71 21.886633597747032 27.75887575442497 28.712110564847077 21.64238008298092 72-73 21.88757655001712 27.50015653038088 28.889646747565116 21.72262017203688 74-75 21.540036061863347 28.255708851409008 28.45749942217092 21.74675566455673 76-77 21.633328526415458 27.901294104442893 28.5237178101636 21.941659558978053 78-79 21.782191407631608 27.814260755114372 28.492202471310037 21.911345365943983 80-81 21.85129776748158 27.944946709837883 28.433120696672038 21.770634826008504 82-83 21.914120106094295 27.888851440012896 28.5546384281419 21.642390025750913 84-85 21.62558757762486 27.92350050723011 28.5218854972154 21.929026417929634 86-87 21.446051412467387 27.99004069115685 28.463660712085254 22.10024718429051 88-89 21.554390202150255 27.79168046018508 28.348694372710746 22.305234964953915 90-91 21.581998658386052 27.513610986579195 28.525850049661035 22.37854030537372 92-93 21.751233017595126 27.520679625910006 28.652834400133486 22.075252956361386 94-95 21.49743990077992 27.408281706164423 28.763665574508646 22.330612818547017 96-97 21.135389183705687 27.517153364164205 29.068233121895627 22.27922433023448 98-99 21.24867115874957 27.535622270713272 28.545574925331778 22.67013164520538 100-101 20.970045029216198 27.54453042744614 28.709647656958577 22.775776886379088 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 167.0 1 129.5 2 138.0 3 191.5 4 183.5 5 200.0 6 360.5 7 827.5 8 1383.5 9 1691.5 10 1653.5 11 1568.5 12 1680.0 13 1850.5 14 2050.0 15 2171.5 16 2216.0 17 2334.0 18 2528.0 19 2691.5 20 2994.5 21 3490.5 22 4081.0 23 4856.0 24 5777.5 25 7112.5 26 9125.5 27 11347.0 28 13308.0 29 15834.5 30 19100.5 31 22391.0 32 26069.5 33 29928.0 34 34499.0 35 38438.5 36 42699.0 37 47018.5 38 48887.0 39 49772.0 40 50585.5 41 52257.5 42 53405.5 43 54747.0 44 56959.5 45 57974.5 46 57783.5 47 60736.5 48 67815.0 49 65519.0 50 56235.0 51 50767.0 52 47075.0 53 44805.5 54 42065.5 55 37710.5 56 32881.0 57 28508.5 58 25081.5 59 21549.5 60 17539.0 61 14034.0 62 11133.5 63 8430.0 64 6112.0 65 4510.5 66 3409.0 67 2647.0 68 1929.0 69 1280.0 70 838.0 71 598.5 72 473.5 73 375.0 74 293.5 75 235.5 76 151.0 77 82.0 78 42.0 79 25.0 80 16.5 81 11.5 82 8.5 83 3.5 84 2.0 85 1.0 86 1.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05401952562730258 2 0.0 3 0.0 4 0.0 5 0.016185875126306443 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0026976458543844072 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.992152847948024E-4 24-25 1.332170792288596E-4 26-27 0.0012322579828669513 28-29 0.0035302525995647796 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.012089449940019011 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.9982561884328943E-4 74-75 0.0 76-77 3.33042698072149E-5 78-79 0.0 80-81 0.0 82-83 1.9982561884328943E-4 84-85 0.0011989537130597365 86-87 0.0 88-89 0.0 90-91 0.008792327229104734 92-93 8.326067451803727E-4 94-95 0.0 96-97 0.0 98-99 6.66085396144298E-5 100-101 0.011489973083489143 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1501309.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.31102824395648 #Duplication Level Percentage of deduplicated Percentage of total 1 79.10791440819156 47.71079660192933 2 12.371754070536344 14.92306418350802 3 3.781264868108805 6.841559167751715 4 1.6215154455366916 3.9118105533510037 5 0.8367502704801852 2.5232634598034336 6 0.4935894744924948 1.7861333242221893 7 0.33833350909106696 1.428366927586777 8 0.2329205327708968 1.1238141464434357 9 0.17153162583625003 0.9310723857487666 >10 0.9528608853770912 10.490181707075582 >50 0.06075028218670283 2.519630823608595 >100 0.028605782214009015 3.0243244587873557 >500 5.522112944821441E-4 0.21214922533077962 >1k 0.001325307106757146 1.355756776258301 >5k 3.313267766892865E-4 1.218076258594652 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7283 0.4851099940118923 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5724 0.3812672807529962 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5280 0.3516930891641894 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3953 0.263303557095841 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2353 0.15672989371275334 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2150 0.1432083601710241 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1701 0.1133011258841451 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1638 0.10910478788843601 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1535 0.10224410830814977 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.660853961442981E-5 0.0 12-13 6.660853961442981E-5 0.0 0.0 6.660853961442981E-5 0.0 14-15 1.3321707922885962E-4 0.0 0.0 6.660853961442981E-5 0.0 16-17 1.3321707922885962E-4 0.0 0.0 9.991280942164472E-5 0.0 18-19 1.9982561884328943E-4 0.0 0.0 1.9982561884328943E-4 0.0 20-21 2.6643415845771924E-4 0.0 0.0 1.9982561884328943E-4 0.0 22-23 2.6643415845771924E-4 0.0 0.0 2.9973842826493417E-4 0.0 24-25 2.6643415845771924E-4 0.0 0.0 3.6634696787936393E-4 0.0 26-27 2.6643415845771924E-4 0.0 0.0 6.327811263370832E-4 0.0 28-29 2.9973842826493417E-4 0.0 0.0 0.0013321707922885962 0.0 30-31 3.3304269807214905E-4 0.0 0.0 0.0027309501241916225 0.0 32-33 3.3304269807214905E-4 0.0 0.0 0.005128857550311095 0.0 34-35 3.6634696787936393E-4 0.0 0.0 0.006760766770864625 0.0 36-37 3.9965123768657886E-4 0.0 0.0 0.009258587006405743 0.0 38-39 4.6625977730100867E-4 0.0 0.0 0.011623190162718 0.0 40-41 4.6625977730100867E-4 0.0 0.0 0.014520661635945698 0.0 42-43 4.6625977730100867E-4 0.0 0.0 0.019816040535292866 0.0 44-45 4.6625977730100867E-4 0.0 0.0 0.029674104398228478 0.0 46-47 4.995640471082235E-4 0.0 0.0 0.05042266448812337 0.0 48-49 5.328683169154385E-4 0.0 0.0 0.0942843878242254 0.0 50-51 5.328683169154385E-4 0.0 0.0 0.12855448145584952 0.0 52-53 5.328683169154385E-4 0.0 0.0 0.16405683307034064 0.0 54-55 5.328683169154385E-4 0.0 0.0 0.21178185170407957 0.0 56-57 5.328683169154385E-4 0.0 0.0 0.2471176819695346 0.0 58-59 5.328683169154385E-4 0.0 0.0 0.28598376483455434 0.0 60-61 5.328683169154385E-4 0.0 0.0 0.3590866370613911 0.0 62-63 5.328683169154385E-4 0.0 0.0 0.40884321615337016 0.0 64-65 5.328683169154385E-4 0.0 0.0 0.461663788067613 0.0 66-67 5.994768565298682E-4 0.0 0.0 0.5752979566498302 0.0 68-69 6.327811263370832E-4 0.0 0.0 0.6998226214590068 0.0 70-71 7.659982055659428E-4 0.0 0.0 0.8175532152275116 0.0 72-73 8.659110149875875E-4 0.0 0.0 0.9522356823278886 0.0 74-75 8.659110149875875E-4 0.0 0.0 1.0756280019636197 0.0 76-77 8.659110149875875E-4 0.0 0.0 1.1756740284644933 0.0 78-79 8.659110149875875E-4 0.0 0.0 1.3131873584984837 0.0 80-81 9.325195546020173E-4 0.0 0.0 1.5370919644123893 0.0 82-83 9.99128094216447E-4 0.0 0.0 1.7232628326347208 0.0 84-85 9.99128094216447E-4 0.0 0.0 1.9283505261075502 0.0 86-87 9.99128094216447E-4 0.0 0.0 2.2845396916957137 0.0 88-89 9.99128094216447E-4 0.0 0.0 2.777409580572687 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6720 0.0 24.116926 1 TACATGG 6740 0.0 23.188372 2 GTATCAA 12940 0.0 22.845093 1 GAGTACT 6520 0.0 22.839197 12-13 ACATGGG 6745 0.0 22.396463 3 GTACTTT 6925 0.0 21.43417 14-15 AGTACTT 6740 0.0 20.89564 12-13 AGAGTAC 10545 0.0 20.24762 10-11 TCAACGC 14805 0.0 19.957964 4 CAACGCA 14825 0.0 19.93104 5 ATCAACG 14830 0.0 19.92432 3 TATCAAC 15015 0.0 19.742107 2 CATGGGA 4235 0.0 19.742107 4 AACGCAG 15100 0.0 19.691273 6 GGTATCA 9950 0.0 18.676285 1 AACGCGA 65 0.007567625 18.268507 76-77 TACTTTT 7825 0.0 17.936989 14-15 ACGCAGA 16510 0.0 17.923872 7 CGCAGAG 16565 0.0 17.778336 8 GTGTAGA 830 0.0 17.750902 1 >>END_MODULE