FastQCFastQC Report
Thu 26 May 2016
SRR937204_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937204_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1638604
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60620.36994905419491225No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT47970.29274919382596404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43770.26711761963232117No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29850.1821672594476762No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28430.17350134626792074No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22550.1376171423968207No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT21220.12950047723550046No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC18890.11528105631378904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG74950.026.1203331
TACATGG73850.026.1193452
ACATGGG74800.024.5808053
GAGTACT54550.022.94806312-13
GACCGTA1050.00219349122.6245887
GTATCAA126250.022.0932621
GTACTTT58550.021.17454114-15
CATGGGA47350.021.0723324
AGTACTT59550.020.46283312-13
AGAGTAC97900.020.16330710-11
GAGTACA61350.019.2858121
CAACGCA143200.019.2773085
TCAACGC143350.019.2571374
ATCAACG143800.019.2287393
CATGGGG30150.019.225864
AACGCAG146150.018.88826
AGTACAT56900.018.8704762
TATCAAC147600.018.733692
TACCTGG12950.018.7105752
GGACCGT2051.586294E-518.5416956
Produced by FastQC (version 0.11.5)