FastQCFastQC Report
Thu 26 May 2016
SRR937204_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937204_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1638604
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73820.45050543023207557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56560.34517186580772413No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT54720.33394279520860437No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40210.24539180912532865No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT25240.15403355539227295No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT22430.13688481170557376No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC17160.10472328884831233No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT16710.10197704875613632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG71850.023.8750341
TACATGG71600.023.3518282
GAGTACT70650.022.35479512-13
ACATGGG73250.021.723433
AGTACTT73300.021.31980312-13
GTATCAA144500.021.1120661
GTACTTT74450.020.98984314-15
AGAGTAC112100.020.19056710-11
GGTATCA113050.018.6627641
CAACGCA163800.018.5591165
ATCAACG164350.018.497013
TCAACGC164200.018.4849784
CATGGGG27850.018.4200064
AACGCAG166850.018.2193036
TATCAAC168150.018.1354922
TACTTTT84850.018.08125914-15
ACTTTTT87200.017.83909416-17
CATGGGA46700.017.6979774
TTAGACG1350.00940235517.5924973
GACGCGA1350.00940235517.5924978
Produced by FastQC (version 0.11.5)