##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937204_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1638604 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27133157248487 33.0 31.0 34.0 31.0 34.0 2 32.472994085209116 34.0 31.0 34.0 31.0 34.0 3 32.53123573480841 34.0 31.0 34.0 31.0 34.0 4 36.147058105558145 37.0 35.0 37.0 35.0 37.0 5 36.080776075244536 37.0 35.0 37.0 35.0 37.0 6 36.12414836043364 37.0 35.0 37.0 35.0 37.0 7 36.072884601770774 37.0 35.0 37.0 35.0 37.0 8 36.05157744030895 37.0 35.0 37.0 35.0 37.0 9 37.77093001115584 39.0 37.0 39.0 35.0 39.0 10-11 37.69670860073575 39.0 37.0 39.0 35.0 39.0 12-13 37.60675916816998 39.0 37.0 39.0 35.0 39.0 14-15 38.829233908863884 40.0 38.0 41.0 35.0 41.0 16-17 38.80539959624168 40.0 38.0 41.0 35.0 41.0 18-19 38.808501321856895 40.0 38.0 41.0 35.0 41.0 20-21 38.71801179540633 40.0 38.0 41.0 34.5 41.0 22-23 38.57269389065326 40.0 38.0 41.0 34.0 41.0 24-25 38.5187226443973 40.0 38.0 41.0 34.0 41.0 26-27 38.48205393127321 40.0 38.0 41.0 34.0 41.0 28-29 38.379227073777436 40.0 38.0 41.0 34.0 41.0 30-31 38.45891960473672 40.0 38.0 41.0 34.0 41.0 32-33 38.40781817937708 40.0 38.0 41.0 34.0 41.0 34-35 38.29081492538771 40.0 38.0 41.0 34.0 41.0 36-37 38.13666785873829 40.0 38.0 41.0 34.0 41.0 38-39 38.022652819106995 40.0 38.0 41.0 33.0 41.0 40-41 37.87301874034239 40.0 37.0 41.0 33.0 41.0 42-43 37.66895265726191 40.0 37.0 41.0 33.0 41.0 44-45 37.39529379886781 40.0 37.0 41.0 32.5 41.0 46-47 37.07568942831825 40.0 37.0 41.0 31.5 41.0 48-49 36.77145393273787 40.0 36.5 41.0 31.0 41.0 50-51 36.553314894873935 39.5 36.0 41.0 30.5 41.0 52-53 36.337461644180046 39.0 36.0 41.0 30.0 41.0 54-55 36.13390849772124 39.0 35.5 40.5 30.0 41.0 56-57 35.88620160819819 39.0 35.0 40.0 29.0 41.0 58-59 35.63972289827194 38.5 35.0 40.0 28.5 41.0 60-61 35.32410789916295 38.0 35.0 40.0 28.0 41.0 62-63 34.9885844902124 38.0 34.0 40.0 27.5 41.0 64-65 34.61936654615758 37.5 34.0 40.0 26.5 41.0 66-67 34.20858608913441 37.0 34.0 39.5 26.0 41.0 68-69 33.77875679541854 36.0 33.0 39.0 26.0 40.0 70-71 33.37183724682718 36.0 33.0 39.0 25.5 40.0 72-73 32.90333051792868 35.0 32.0 38.0 24.5 40.0 74-75 32.43783397330898 35.0 32.0 37.0 23.5 39.0 76-77 31.151643411098718 34.0 30.5 36.0 22.5 38.5 78-79 31.7588175666604 35.0 31.5 36.0 23.0 39.0 80-81 31.55856082372556 35.0 31.5 36.0 22.5 38.0 82-83 31.21122858237866 34.0 31.0 35.5 21.5 37.0 84-85 30.839031578099405 34.0 31.0 35.0 20.0 37.0 86-87 30.44887629958184 34.0 31.0 35.0 18.5 36.0 88-89 29.993109988746518 34.0 30.0 35.0 16.0 36.0 90-91 29.50411203683135 34.0 29.5 35.0 8.5 35.5 92-93 29.169165033162372 34.0 29.0 35.0 4.5 35.0 94-95 28.744738814258966 34.0 29.0 35.0 2.0 35.0 96-97 28.29284226085131 34.0 29.0 35.0 2.0 35.0 98-99 27.574141769457416 33.0 28.0 35.0 2.0 35.0 100-101 25.057095246929705 31.0 22.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.05848121205182366 1101 2 -0.0021969343006631448 1101 3 0.0016948429746364013 1101 4 0.025690086200953033 1101 5 -0.01430809425068702 1101 6 -0.030736447530884448 1101 7 -0.038078265003235856 1101 8 -0.0013248861086623265 1101 9 -0.05753783115270039 1101 10-11 -0.011550988755679725 1101 12-13 -0.06271553120130591 1101 14-15 -0.14094175113182672 1101 16-17 0.007599123722776824 1101 18-19 -0.03802158782324483 1101 20-21 -0.030006914283625008 1101 22-23 -0.13370475546235383 1101 24-25 -0.1601376418725451 1101 26-27 -0.12046197376378842 1101 28-29 -0.1938796982539941 1101 30-31 -0.09860112137858579 1101 32-33 -0.004230277804687432 1101 34-35 -0.21838577637528545 1101 36-37 -0.1065922714628158 1101 38-39 -0.18575100879820639 1101 40-41 -0.11570088867291162 1101 42-43 -0.34670571540340944 1101 44-45 -0.18016562781615875 1101 46-47 -0.15274712132731594 1101 48-49 -0.32137424478191434 1101 50-51 -0.23310025955169777 1101 52-53 -0.20497139692460564 1101 54-55 -0.260878442761765 1101 56-57 -0.25762429612938575 1101 58-59 -0.20219988762832486 1101 60-61 -0.22730087668656296 1101 62-63 -0.20428717099751026 1101 64-65 -0.282249838777453 1101 66-67 -0.27378555349324785 1101 68-69 -0.22066910934821493 1101 70-71 -0.1961558364761231 1101 72-73 -0.11215440335739402 1101 74-75 -0.1854512115869582 1101 76-77 -0.10989969769768848 1101 78-79 -0.27252069177882987 1101 80-81 -0.31558437080391144 1101 82-83 -0.09417322945285278 1101 84-85 -0.1307863820853612 1101 86-87 -0.3336463968801695 1101 88-89 -0.1410017356946689 1101 90-91 -0.23270306449059674 1101 92-93 -0.27737421196410494 1101 94-95 -0.23774022314323418 1101 96-97 -0.3577981441523086 1101 98-99 -0.05465377509322167 1101 100-101 0.6607783801017497 1102 1 -0.07398080806583351 1102 2 0.006042135633805401 1102 3 0.017766752481776393 1102 4 0.038413400681612586 1102 5 -0.05759070489796159 1102 6 -0.05683843155099311 1102 7 -0.03211273847482232 1102 8 -0.10889962351776461 1102 9 -0.1812540534347704 1102 10-11 -0.09702095913003461 1102 12-13 -0.18160075429134537 1102 14-15 -0.1334579105716358 1102 16-17 -0.2436038567961205 1102 18-19 -0.18600165784747702 1102 20-21 -0.051635875590740454 1102 22-23 0.0431883677982583 1102 24-25 -0.14789237748615847 1102 26-27 -0.11139697690590111 1102 28-29 -0.13674373452295896 1102 30-31 0.0033671881644679047 1102 32-33 0.056036577388780984 1102 34-35 -0.062053295901272065 1102 36-37 0.05481696635027333 1102 38-39 0.06486282547775346 1102 40-41 0.20631522587955686 1102 42-43 0.051882577980194355 1102 44-45 0.08274396909558845 1102 46-47 0.24507135429886517 1102 48-49 0.3784574277201074 1102 50-51 0.31805558926776456 1102 52-53 0.19158574465004108 1102 54-55 0.24043114517251496 1102 56-57 0.1075666994716542 1102 58-59 0.19147436499758186 1102 60-61 0.2249188019201327 1102 62-63 0.20071664442578196 1102 64-65 0.19558816283848302 1102 66-67 0.16719658134829984 1102 68-69 0.1457540435380409 1102 70-71 0.048172649025275405 1102 72-73 -0.002353254241675984 1102 74-75 0.20535361829004728 1102 76-77 0.3236300177166207 1102 78-79 0.255288133070799 1102 80-81 0.15893645691850367 1102 82-83 0.12268869711146735 1102 84-85 0.17296058801967362 1102 86-87 0.15485010190787207 1102 88-89 0.23245189569055213 1102 90-91 0.23390637082675525 1102 92-93 0.28597303644239247 1102 94-95 0.28839318200983044 1102 96-97 0.38738432285550317 1102 98-99 0.4210487116650157 1102 100-101 0.8265506666624844 1103 1 -0.04567080489245967 1103 2 -0.005428428403668306 1103 3 -0.037690565666387954 1103 4 -0.034319989067221 1103 5 0.03118025992462492 1103 6 -0.11546500795286363 1103 7 -0.06333607333608171 1103 8 -0.13942569805674765 1103 9 -0.022192901383249364 1103 10-11 -0.08998621070790591 1103 12-13 -0.05223354815146308 1103 14-15 -0.06540539126695677 1103 16-17 -0.08122844102563676 1103 18-19 -0.11920306118305035 1103 20-21 -0.13168785424687712 1103 22-23 0.033858066798060804 1103 24-25 -0.10597492629144512 1103 26-27 -0.12136430292872546 1103 28-29 -0.23017955601491025 1103 30-31 -0.16037733189241976 1103 32-33 -0.17658774239308173 1103 34-35 -0.10233572599894813 1103 36-37 -0.01857739562567673 1103 38-39 -0.08472125712393819 1103 40-41 0.007710774931659614 1103 42-43 0.06306136810278673 1103 44-45 -0.19257895087667265 1103 46-47 -0.024732689987310152 1103 48-49 0.10275972665173327 1103 50-51 0.22211295090287564 1103 52-53 0.24840085460149908 1103 54-55 0.014311209345152065 1103 56-57 0.00791606420961699 1103 58-59 -0.09206905730769677 1103 60-61 -0.04208929605477607 1103 62-63 -0.24885245280868418 1103 64-65 -0.3258038896279274 1103 66-67 -0.0514206806194224 1103 68-69 -0.25685487951357544 1103 70-71 -0.22811888394841162 1103 72-73 -0.1689285395513096 1103 74-75 0.03568018176336096 1103 76-77 0.09472603572256943 1103 78-79 -0.060931910886282026 1103 80-81 -0.1616942801264969 1103 82-83 -0.19794997336324016 1103 84-85 -0.18866805695052946 1103 86-87 -0.11288023200136976 1103 88-89 0.03003631325928069 1103 90-91 0.16565081448765184 1103 92-93 0.10235968242337634 1103 94-95 0.3240404323418602 1103 96-97 0.47705191215066023 1103 98-99 0.34906444336229825 1103 100-101 0.721942666239368 1104 1 -0.035273062386771414 1104 2 -0.024541698453241167 1104 3 0.013577457253902026 1104 4 -0.02739785474752665 1104 5 -0.00684422049876332 1104 6 -3.849035604091E-4 1104 7 -0.04146788470867335 1104 8 -0.05436220981604123 1104 9 -0.004444973100795835 1104 10-11 -0.005668252995029377 1104 12-13 -0.08265973653954717 1104 14-15 -0.03736181059985455 1104 16-17 -0.16095325210589806 1104 18-19 -0.11539452408601392 1104 20-21 -0.1984629727335161 1104 22-23 -0.2233072510369638 1104 24-25 -0.1129278088226684 1104 26-27 -0.3750050880835474 1104 28-29 -0.12393751760539828 1104 30-31 -0.07928384672653976 1104 32-33 0.021585238259781647 1104 34-35 -0.04339806913383626 1104 36-37 -0.013498487838511153 1104 38-39 0.043816112555909115 1104 40-41 0.017056565077389507 1104 42-43 0.06140808372423834 1104 44-45 0.18281899611220354 1104 46-47 0.11574740622581459 1104 48-49 0.17576703806144423 1104 50-51 0.13086619423332024 1104 52-53 0.33333862355367927 1104 54-55 0.29698921195345207 1104 56-57 0.3434500909681404 1104 58-59 0.22176824375667792 1104 60-61 0.2512625770868837 1104 62-63 0.3586928250688075 1104 64-65 0.24909481546340118 1104 66-67 0.11918660413173399 1104 68-69 0.3075809513924739 1104 70-71 0.42241678624900203 1104 72-73 0.42229019534912027 1104 74-75 0.4196805089364517 1104 76-77 0.5082906925467761 1104 78-79 0.2785516706290423 1104 80-81 0.26122873877229225 1104 82-83 0.17536936423242722 1104 84-85 0.32365303920049016 1104 86-87 0.38300450613141024 1104 88-89 0.4096782707843367 1104 90-91 0.28432891403612004 1104 92-93 0.40515155944265047 1104 94-95 0.5105772597625986 1104 96-97 0.42608561552281543 1104 98-99 0.44357187367518947 1104 100-101 1.112247437059164 1105 1 0.04057580829515217 1105 2 0.006321547635728564 1105 3 0.07767297374842741 1105 4 0.07419340376954153 1105 5 -0.06932831340775181 1105 6 0.017085054496838836 1105 7 -0.0729711267111739 1105 8 -0.06580046624740987 1105 9 0.06011413208096883 1105 10-11 0.06264009970895046 1105 12-13 0.02208829068418794 1105 14-15 0.10572063542591792 1105 16-17 0.08142359471293048 1105 18-19 -0.05959653233676221 1105 20-21 -0.06757460197898268 1105 22-23 -0.06173515972929522 1105 24-25 0.07209085203265175 1105 26-27 0.059932125496004574 1105 28-29 0.016775013158209617 1105 30-31 -0.09458227340572734 1105 32-33 -0.0667143000225181 1105 34-35 -0.014208167348705558 1105 36-37 -0.01351007013114014 1105 38-39 -0.0594036460037799 1105 40-41 0.06923612981483274 1105 42-43 -0.01674417828266428 1105 44-45 0.06084498143120243 1105 46-47 -0.04617720255254909 1105 48-49 0.12226441912689268 1105 50-51 0.11061277367047495 1105 52-53 0.14730383925606105 1105 54-55 0.2254280169103069 1105 56-57 0.08750117303640081 1105 58-59 0.18186576072102412 1105 60-61 0.03232442979635408 1105 62-63 0.05301845201979205 1105 64-65 0.15161274648580303 1105 66-67 0.10858732146172656 1105 68-69 0.18694023678283855 1105 70-71 0.3679794169024575 1105 72-73 0.36968728296678677 1105 74-75 0.488417851940703 1105 76-77 0.428394107570913 1105 78-79 0.23829815503200535 1105 80-81 0.13343996771590483 1105 82-83 0.01347489911309907 1105 84-85 0.06037253003102805 1105 86-87 0.16038764451100107 1105 88-89 0.3410662541260301 1105 90-91 0.5522374400587111 1105 92-93 0.5798686619450493 1105 94-95 0.779944857675293 1105 96-97 0.7000277735534013 1105 98-99 0.7499726643596993 1105 100-101 1.2794837149379639 1106 1 -0.15206863215887267 1106 2 -0.10675158772533422 1106 3 -0.06223220524506701 1106 4 -0.08027545562126903 1106 5 -0.15745844402776754 1106 6 -0.09883575039313541 1106 7 -0.1385171476497078 1106 8 -0.12048862286364681 1106 9 -0.22674747992576272 1106 10-11 -0.2587444656244742 1106 12-13 -0.18665577912620535 1106 14-15 -0.17607924554385335 1106 16-17 -0.056695975773394025 1106 18-19 -0.13200741550819117 1106 20-21 -0.06329720510000669 1106 22-23 -0.16017529927813 1106 24-25 -0.1303188433175677 1106 26-27 -0.2452589943154564 1106 28-29 -0.2719157814755633 1106 30-31 -0.15217500952240215 1106 32-33 -0.19851438258073983 1106 34-35 -0.09480044043221625 1106 36-37 -0.23919795972021518 1106 38-39 -0.21488913147994992 1106 40-41 -0.18019066116678317 1106 42-43 -0.2988666007804355 1106 44-45 -0.27573330575543764 1106 46-47 -0.30629609011922554 1106 48-49 -0.46478783353105513 1106 50-51 -0.1946273476423599 1106 52-53 -0.1582226927309094 1106 54-55 -0.08214386255468042 1106 56-57 -0.13348862007492102 1106 58-59 -0.27275140574544565 1106 60-61 -0.12887004072536712 1106 62-63 -0.14562308990237938 1106 64-65 -0.1510467324669733 1106 66-67 -0.021200132975401687 1106 68-69 -0.14618577057793658 1106 70-71 -0.2251297934879659 1106 72-73 -0.020827546035029343 1106 74-75 -0.03184349353507798 1106 76-77 0.09760993778505167 1106 78-79 -0.0037401089378406027 1106 80-81 -0.10529147025176044 1106 82-83 -0.11597388671964026 1106 84-85 -0.011681696643094597 1106 86-87 0.10683365555619062 1106 88-89 0.3161922546638074 1106 90-91 0.37306825741305616 1106 92-93 0.4405538269886158 1106 94-95 0.5685373391702484 1106 96-97 0.6596795341494293 1106 98-99 0.6792723367629527 1106 100-101 1.2764546157995547 1107 1 -0.031052904928792202 1107 2 -0.11331140570653275 1107 3 -0.08089369090899368 1107 4 -0.04930339087392355 1107 5 -0.1114552040018637 1107 6 -0.06529312582021873 1107 7 -0.09844319301386406 1107 8 -0.0720221275043329 1107 9 -0.10984160970532031 1107 10-11 -0.06539743909842599 1107 12-13 -0.13803782300139744 1107 14-15 -0.09618440394617522 1107 16-17 -0.015040466874722824 1107 18-19 -0.14723848101331072 1107 20-21 -0.013189330849478154 1107 22-23 0.03642884590390594 1107 24-25 -0.11326834943202613 1107 26-27 -0.1788546064163654 1107 28-29 -0.1487069593370407 1107 30-31 -0.17888765568916654 1107 32-33 -0.22663385711159378 1107 34-35 -0.11811922593528124 1107 36-37 -0.15089775783582837 1107 38-39 -0.22912786783186334 1107 40-41 -0.16989750149580374 1107 42-43 -0.156704766614304 1107 44-45 -0.0026637895372658704 1107 46-47 0.017936777721523356 1107 48-49 -0.18800444236724445 1107 50-51 -0.15538524022012012 1107 52-53 -0.10160675355071902 1107 54-55 -0.2275219826939292 1107 56-57 -0.07240909889984692 1107 58-59 -0.20798127475800499 1107 60-61 -0.20887598520431538 1107 62-63 -0.15256274679389747 1107 64-65 -0.01153332510303784 1107 66-67 -0.1469315602770891 1107 68-69 -0.012980063118469332 1107 70-71 0.03477972888359204 1107 72-73 -0.07096628219702694 1107 74-75 0.03259950119085886 1107 76-77 0.007344544338412362 1107 78-79 0.04452934498674921 1107 80-81 -0.02881138045784226 1107 82-83 -0.03183482009779226 1107 84-85 -0.001284081271773374 1107 86-87 0.05928542076256349 1107 88-89 0.11060586885002976 1107 90-91 0.3644796248521267 1107 92-93 0.49411586132985263 1107 94-95 0.3396981648640711 1107 96-97 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2316 7 -0.06476992876326904 2316 8 -0.038480449415253304 2316 9 0.027240101645809034 2316 10-11 0.0212547896421853 2316 12-13 -0.10788905753545208 2316 14-15 0.05979362847519809 2316 16-17 0.09230003059407466 2316 18-19 3.838301514136333E-4 2316 20-21 0.0655677281998095 2316 22-23 0.16067816512827449 2316 24-25 0.1265136196086729 2316 26-27 0.24246966209251042 2316 28-29 0.3385830967698453 2316 30-31 0.11381022613914382 2316 32-33 0.06843714458806005 2316 34-35 0.08648720351077799 2316 36-37 -0.01071097858989134 2316 38-39 -0.017075854746352093 2316 40-41 -0.3477407677567399 2316 42-43 -0.07485360518366946 2316 44-45 -0.3522909620796213 2316 46-47 -0.46829327875087046 2316 48-49 -0.6597636762433652 2316 50-51 -0.5259135952103335 2316 52-53 -0.40407033890809174 2316 54-55 -0.30579473156539194 2316 56-57 -0.37234787478303133 2316 58-59 -0.24882963998270213 2316 60-61 -0.4775886339240927 2316 62-63 -0.3067926936111576 2316 64-65 -0.48184145603126893 2316 66-67 -0.40217033433268057 2316 68-69 -0.3622645633651942 2316 70-71 -0.17861281822242603 2316 72-73 -0.1815242440735929 2316 74-75 -0.21298870722700514 2316 76-77 -0.3570860713751678 2316 78-79 -0.5174413405406 2316 80-81 -0.2880425152057242 2316 82-83 -0.22602127037832886 2316 84-85 -0.14950949567680283 2316 86-87 -0.25019243803817304 2316 88-89 -0.2363365804801596 2316 90-91 -0.19334426900170243 2316 92-93 -0.2278974069414339 2316 94-95 -0.08560384365247842 2316 96-97 0.00660425091803063 2316 98-99 -0.11539816528040703 2316 100-101 -0.2162097608120952 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 8.0 4 17.0 5 35.0 6 52.0 7 140.0 8 294.0 9 598.0 10 1046.0 11 1739.0 12 2776.0 13 4152.0 14 5345.0 15 7602.0 16 10578.0 17 15056.0 18 13380.0 19 8031.0 20 6890.0 21 6761.0 22 7129.0 23 7855.0 24 9014.0 25 10676.0 26 13232.0 27 16754.0 28 21161.0 29 26758.0 30 33797.0 31 43102.0 32 55559.0 33 74211.0 34 103897.0 35 149562.0 36 235266.0 37 376280.0 38 335266.0 39 34582.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.722705374192905 20.13195636548396 15.25663033713972 21.888707923183418 2 15.36240604807507 21.09710460855704 39.9338705385804 23.60661880478749 3 19.64672367454248 25.085560635760686 30.10355155974232 25.164164129954525 4 12.64259088834154 17.188350571584106 38.10524080253679 32.06381773753756 5 13.07514151015364 38.412828219642066 34.40465038219184 14.10737988801245 6 31.116853126197665 37.33074006898555 17.392243641538773 14.16016316327801 7 28.085675367568978 33.35027865182802 20.710861196481883 17.85318478412112 8 26.303365547746743 35.15455839238767 19.570134089749565 18.971941970116028 9 26.063100053460143 17.202692047621024 20.793126344131956 35.94108155478688 10-11 24.589276532899223 27.410613617396535 27.33271186880284 20.66739798090141 12-13 25.78401880241414 24.56611574030844 28.15986202123511 21.490003436042315 14-15 22.4647321744607 25.991575755948354 26.970427266136298 24.57326480345465 16-17 21.31274548335046 29.436520355131563 27.487300165262624 21.763433996255348 18-19 21.203384100124254 28.028401004757708 30.05076272241493 20.717452172703105 20-21 22.183829095934435 26.684368732277207 30.1790823710556 20.95271980073276 22-23 21.737133755240787 26.710586540867453 30.14472808939393 21.40755161449783 24-25 21.739485990220235 26.860604026333657 30.267013510111607 21.132896473334505 26-27 21.562263803199873 26.970306600801358 30.412795340402592 21.054634255596177 28-29 21.179326061032157 27.483970544616998 30.331312029079438 21.005391365271407 30-31 21.50791851441157 26.92471880661285 30.187022857671188 21.38033982130439 32-33 21.399221532475206 27.015007896965955 30.176540518636596 21.409230051922247 34-35 21.947035403306717 26.952881843325173 30.024154707299626 21.075928046068483 36-37 21.780857363951267 26.765191589914345 30.25316061720831 21.200790428926087 38-39 21.400372148544513 27.30449232969993 30.26529467659144 21.029840845164113 40-41 21.557180585285327 27.244465939726076 30.010512006420104 21.187841468568493 42-43 21.475300822242886 27.205671196230192 29.66806631776607 21.650961663760857 44-45 21.681747126128137 27.057130189530753 29.57653776899114 21.68458491534997 46-47 21.932480330818187 27.565537494110842 29.199458807619166 21.302523367451805 48-49 21.79806713519557 27.612986420147884 29.083018227710905 21.505928216945644 50-51 21.707898918835788 27.72060851798238 29.161896345913963 21.40959621726787 52-53 22.000770167776963 28.04487844531077 28.4511083825012 21.503243004411072 54-55 21.64143990860513 27.749016846047002 28.70647819729477 21.9030650480531 56-57 21.94145138178596 27.848735875171794 28.56678611793942 21.64302662510283 58-59 22.254522752294026 27.590711361622454 28.59659807982893 21.55816780625459 60-61 21.90480433344481 27.84681350710727 29.105506882687948 21.142875276759973 62-63 21.678636204964715 27.772665024618515 29.097329189969024 21.451369580447746 64-65 21.56978278746676 27.897125751974627 28.831641597031616 21.701449863527 66-67 21.74677347302948 27.77113933567842 28.435515841533405 22.046571349758697 68-69 21.711774168743638 27.699950689733456 28.696500191626527 21.891774949896377 70-71 21.936874314965664 27.756736832083895 28.581554786879565 21.724834066070876 72-73 21.913165108836548 27.47784089383402 28.800186011995578 21.808807985333857 74-75 21.61629655487232 28.189147591486414 28.355813851302695 21.838742002338577 76-77 21.700819722153735 27.808518714710818 28.448453683745434 22.042207879390016 78-79 21.812805290356913 27.73668927941101 28.441008321717753 22.00949710851432 80-81 21.940322371970286 27.96038579180815 28.267506975449834 21.83178486077173 82-83 22.017338587006773 27.79571823377219 28.455000749115328 21.731942430105708 84-85 21.73844453517247 27.917572166920845 28.372504038515878 21.971479259390808 86-87 21.565216489157844 27.958097258398002 28.313552267661983 22.163133984782167 88-89 21.67701897468821 27.731776561023896 28.240532794988905 22.35067166929899 90-91 21.67787395651663 27.52528697223125 28.399623422854326 22.397215648397793 92-93 21.849232093106995 27.46018571354464 28.5598503550406 22.130731838307767 94-95 21.604396181139553 27.434663896829253 28.643497757847534 22.31744216418366 96-97 21.24921579588479 27.449371538211796 28.923156540567458 22.37825612533596 98-99 21.296409592930562 27.565593241802084 28.425136663711914 22.71286050155544 100-101 21.14837001087341 27.38350604751402 28.65605814939632 22.81206579221625 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 191.0 1 141.0 2 132.0 3 199.0 4 190.5 5 181.0 6 362.0 7 823.5 8 1390.0 9 1733.0 10 1706.0 11 1634.5 12 1726.0 13 1847.0 14 2032.0 15 2202.0 16 2336.0 17 2467.5 18 2620.0 19 2861.5 20 3113.0 21 3469.5 22 4143.0 23 5043.5 24 6011.0 25 7527.5 26 9757.0 27 12209.0 28 14350.5 29 17018.0 30 20614.0 31 24069.5 32 28097.5 33 32435.0 34 37487.0 35 41587.0 36 45879.0 37 50743.0 38 53037.0 39 54209.0 40 55084.0 41 56892.5 42 58378.0 43 59972.5 44 62167.0 45 63375.0 46 63406.5 47 66393.5 48 73591.5 49 71234.5 50 61729.0 51 55679.5 52 51743.0 53 49468.5 54 46547.0 55 41921.0 56 36589.5 57 31805.0 58 28085.0 59 24056.0 60 19559.5 61 15564.5 62 12446.0 63 9600.5 64 6855.0 65 4993.0 66 3726.5 67 2923.0 68 2186.5 69 1522.0 70 1043.0 71 685.0 72 493.5 73 398.0 74 309.0 75 245.0 76 177.0 77 100.0 78 68.0 79 46.0 80 22.5 81 17.0 82 11.5 83 6.0 84 3.0 85 2.5 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0667031204610754 2 0.0 3 0.0 4 0.0 5 0.001769799170513437 6 0.0 7 0.0 8 0.0 9 0.0 10-11 9.154133640586743E-5 12-13 0.005553507741955958 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.051377880195581E-5 22-23 8.543858064547627E-4 24-25 0.0010069547004645418 26-27 4.577066820293372E-4 28-29 0.003539598341026874 30-31 4.577066820293372E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 6.713031336430279E-4 40-41 9.154133640586743E-5 42-43 0.013395548894058602 44-45 2.7462400921760227E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 9.154133640586743E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.7462400921760227E-4 84-85 4.2719290322738136E-4 86-87 0.0 88-89 0.0 90-91 0.009855950553031727 92-93 0.0036006258986307858 94-95 0.0 96-97 0.0 98-99 9.154133640586743E-5 100-101 0.012419107972396017 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1638604.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.07716686541674 #Duplication Level Percentage of deduplicated Percentage of total 1 78.16157162874617 46.17564209574661 2 12.76206795473979 15.078936362199006 3 3.9790482759725236 7.052126968955329 4 1.7674136335971136 4.176551606089168 5 0.880974339949939 2.6022734042686464 6 0.54258646534168 1.923268269114425 7 0.3665297146726614 1.5157475980395283 8 0.24725672377926178 1.1685781383442964 9 0.18611108301126333 0.9895423955903848 >10 1.008503529856488 10.840867790521571 >50 0.06545026050124779 2.645035527656824 >100 0.03011457888346654 3.1115203790198107 >500 8.249777211703332E-4 0.3029635301422771 >1k 0.0012374665817554997 1.2892855798111824 >5k 3.093666454388749E-4 1.1276603545010153 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7382 0.45050543023207557 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5656 0.34517186580772413 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5472 0.33394279520860437 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4021 0.24539180912532865 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2524 0.15403355539227295 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2243 0.13688481170557376 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1716 0.10472328884831233 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1671 0.10197704875613632 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 6.102755760391162E-5 0.0 6 0.0 0.0 0.0 6.102755760391162E-5 0.0 7 0.0 0.0 0.0 6.102755760391162E-5 0.0 8 0.0 0.0 0.0 6.102755760391162E-5 0.0 9 0.0 0.0 0.0 1.8308267281173487E-4 0.0 10-11 0.0 0.0 0.0 2.1359645161369068E-4 0.0 12-13 6.102755760391162E-5 0.0 0.0 3.6616534562346973E-4 0.0 14-15 6.102755760391162E-5 0.0 0.0 4.577066820293372E-4 0.0 16-17 6.102755760391162E-5 0.0 0.0 4.88220460831293E-4 0.0 18-19 6.102755760391162E-5 0.0 0.0 4.88220460831293E-4 0.0 20-21 6.102755760391162E-5 0.0 0.0 6.40789354841072E-4 0.0 22-23 1.2205511520782324E-4 0.0 0.0 7.018169124449837E-4 0.0 24-25 1.2205511520782324E-4 0.0 0.0 7.323306912469395E-4 0.0 26-27 1.2205511520782324E-4 0.0 0.0 9.76440921662586E-4 0.0 28-29 1.2205511520782324E-4 0.0 0.0 0.0014951751612958348 0.0 30-31 1.2205511520782324E-4 0.0 0.0 0.002929322764987758 0.0 32-33 1.2205511520782324E-4 0.0 0.0 0.004699121935501194 0.0 34-35 1.2205511520782324E-4 0.0 0.0 0.007018169124449837 0.0 36-37 1.2205511520782324E-4 0.0 0.0 0.010008519447041507 0.0 38-39 1.8308267281173487E-4 0.0 0.0 0.012724245760415573 0.0 40-41 1.8308267281173487E-4 0.0 0.0 0.016630009447065917 0.0 42-43 1.8308267281173487E-4 0.0 0.0 0.02346509589870402 0.0 44-45 1.8308267281173487E-4 0.0 0.0 0.0339008082489729 0.0 46-47 2.441102304156465E-4 0.0 0.0 0.05602329788039087 0.0 48-49 2.441102304156465E-4 0.0 0.0 0.09813231262708988 0.0 50-51 2.441102304156465E-4 0.0 0.0 0.13334521336454688 0.0 52-53 2.441102304156465E-4 0.0 0.0 0.1670324251619061 0.0 54-55 2.441102304156465E-4 0.0 0.0 0.21643423304227255 0.0 56-57 3.051377880195581E-4 0.0 0.0 0.2553393010147662 0.0 58-59 3.051377880195581E-4 0.0 0.0 0.30062174875686865 0.0 60-61 3.051377880195581E-4 0.0 0.0 0.37217656004745503 0.0 62-63 3.051377880195581E-4 0.0 0.0 0.4220055608310489 0.0 64-65 3.051377880195581E-4 0.0 0.0 0.4782424551630534 0.0 66-67 3.6616534562346973E-4 0.0 0.0 0.5878479486196787 0.0 68-69 3.6616534562346973E-4 0.0 0.0 0.7113066976523919 0.0 70-71 3.6616534562346973E-4 0.0 0.0 0.82454333078645 0.0 72-73 3.6616534562346973E-4 0.0 0.0 0.959078581524273 0.0 74-75 3.6616534562346973E-4 0.0 0.0 1.0822321927689669 0.0 76-77 3.6616534562346973E-4 0.0 0.0 1.1895796665942473 0.0 78-79 3.966791244254255E-4 0.0 0.0 1.3259762578389898 0.0 80-81 4.88220460831293E-4 0.0 0.0 1.5452787860886463 0.0 82-83 4.88220460831293E-4 0.0 0.0 1.7364476102828994 0.0 84-85 5.797617972371604E-4 0.0 0.0 1.936251833878106 0.0 86-87 6.713031336430279E-4 0.0 0.0 2.2853294633724808 0.0 88-89 6.713031336430279E-4 0.0 0.0 2.7304949823142137 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7185 0.0 23.875034 1 TACATGG 7160 0.0 23.351828 2 GAGTACT 7065 0.0 22.354795 12-13 ACATGGG 7325 0.0 21.72343 3 AGTACTT 7330 0.0 21.319803 12-13 GTATCAA 14450 0.0 21.112066 1 GTACTTT 7445 0.0 20.989843 14-15 AGAGTAC 11210 0.0 20.190567 10-11 GGTATCA 11305 0.0 18.662764 1 CAACGCA 16380 0.0 18.559116 5 ATCAACG 16435 0.0 18.49701 3 TCAACGC 16420 0.0 18.484978 4 CATGGGG 2785 0.0 18.420006 4 AACGCAG 16685 0.0 18.219303 6 TATCAAC 16815 0.0 18.135492 2 TACTTTT 8485 0.0 18.081259 14-15 ACTTTTT 8720 0.0 17.839094 16-17 CATGGGA 4670 0.0 17.697977 4 TTAGACG 135 0.009402355 17.592497 3 GACGCGA 135 0.009402355 17.592497 8 >>END_MODULE