FastQCFastQC Report
Thu 26 May 2016
SRR937203_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937203_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359889
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49540.3642944387372793No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36440.26796304698398177No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25720.18913308365609252No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21470.15788053289643492No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18730.1377318295831498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA75500.030.911931
TATCACG804.483781E-429.6991142
GGTATCA51000.027.8671551
ACCGTAT700.0083851427.147478
GTACATG60550.026.9259821
TACATGG60850.026.238692
TCAACGC87700.026.2265574
ATCAACG87850.026.1257533
CAACGCA88400.026.02085
AACGCAG89900.025.6300496
TATCAAC89850.025.4913272
ACATGGG61600.024.6849773
ACGCAGA100300.022.6883017
CGCAGAG100600.022.6678688
GAGTACT46950.022.6620812-13
GTACCTG11050.022.3682841
AGTACTT49400.022.0669912-13
TACCTGG11200.021.6379262
AGAGTAC78500.021.48335510-11
GCAGAGT105500.021.2097649
Produced by FastQC (version 0.11.5)