##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937203_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1359889 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.65941190788366 33.0 31.0 34.0 30.0 34.0 2 31.76883701537405 33.0 31.0 34.0 30.0 34.0 3 31.810176418810652 33.0 31.0 34.0 30.0 34.0 4 35.430797660691425 37.0 35.0 37.0 33.0 37.0 5 35.42677380286185 37.0 35.0 37.0 33.0 37.0 6 35.464456290182504 37.0 35.0 37.0 33.0 37.0 7 35.39967894438443 37.0 35.0 37.0 33.0 37.0 8 35.378641197921304 37.0 35.0 37.0 33.0 37.0 9 36.97280072123534 39.0 37.0 39.0 33.0 39.0 10-11 36.844840277405 39.0 37.0 39.0 33.0 39.0 12-13 36.69004749652362 39.0 37.0 39.0 32.5 39.0 14-15 37.78609982138248 40.0 38.0 41.0 32.5 41.0 16-17 37.70156424531709 40.0 37.0 41.0 32.5 41.0 18-19 37.60303083560497 40.0 37.0 41.0 32.0 41.0 20-21 37.45958236297227 40.0 37.0 41.0 32.0 41.0 22-23 37.53893442773638 40.0 37.0 41.0 32.0 41.0 24-25 37.498414944160885 40.0 37.0 41.0 32.0 41.0 26-27 37.40805278960268 40.0 37.0 41.0 32.0 41.0 28-29 37.25959765833829 40.0 37.0 41.0 31.5 41.0 30-31 37.006052332212406 39.0 36.5 41.0 31.0 41.0 32-33 36.7708801968396 39.0 36.0 41.0 30.5 41.0 34-35 36.783591160749154 39.0 36.0 41.0 30.0 41.0 36-37 36.64272231042386 39.0 36.0 41.0 30.0 41.0 38-39 36.4925699818147 39.0 35.5 41.0 30.0 41.0 40-41 36.26165076708467 39.0 35.0 40.5 30.0 41.0 42-43 35.989989991830214 39.0 35.0 40.0 29.0 41.0 44-45 35.73567879437219 39.0 35.0 40.0 28.5 41.0 46-47 35.339357844647616 38.0 35.0 40.0 26.5 41.0 48-49 34.98911602344015 38.0 34.0 40.0 25.5 41.0 50-51 34.02043181465546 37.5 33.5 39.5 23.5 40.5 52-53 34.13354472313549 38.0 33.5 39.5 24.0 40.0 54-55 34.4068276896129 38.0 34.0 40.0 24.0 41.0 56-57 34.120958401751906 38.0 33.5 40.0 23.0 41.0 58-59 33.82215607303243 38.0 33.0 40.0 22.0 41.0 60-61 33.45061876373734 37.0 33.0 40.0 20.5 41.0 62-63 33.0315588257571 37.0 32.0 39.5 18.5 41.0 64-65 32.57095358518232 36.0 31.5 39.0 17.5 41.0 66-67 32.09757708165887 36.0 31.0 39.0 13.5 40.0 68-69 31.572994928262528 35.0 31.0 38.5 8.0 40.0 70-71 31.06949537793158 35.0 30.0 38.0 7.5 40.0 72-73 30.54096400515042 35.0 29.5 37.0 4.0 39.0 74-75 29.960913721634633 34.0 29.0 36.5 2.0 39.0 76-77 29.393068478383164 34.0 29.0 36.0 2.0 39.0 78-79 28.803100105964532 34.0 27.5 35.5 2.0 37.5 80-81 28.17249128421511 33.5 26.5 35.0 2.0 37.0 82-83 27.564227668581776 33.0 26.0 35.0 2.0 36.5 84-85 26.930939584039578 32.5 25.0 35.0 2.0 36.0 86-87 26.273133321910834 32.0 24.0 35.0 2.0 36.0 88-89 25.643890052791072 32.0 21.5 34.5 2.0 35.0 90-91 25.0088613850101 31.0 19.0 34.0 2.0 35.0 92-93 24.265388572155523 31.0 13.5 34.0 2.0 35.0 94-95 23.398801666900752 31.0 4.5 34.0 2.0 35.0 96-97 22.45252737539608 30.0 2.0 34.0 2.0 35.0 98-99 21.182814920923693 29.5 2.0 34.0 2.0 35.0 100-101 18.158126876531835 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15979314618113705 1101 2 0.13658159978022155 1101 3 0.11347181466554446 1101 4 -0.007225357470851179 1101 5 0.07202604722695582 1101 6 0.03548444099212844 1101 7 0.1266321599656095 1101 8 0.030022052462932436 1101 9 0.0663537073174254 1101 10-11 0.1888233690812413 1101 12-13 0.24099298691795212 1101 14-15 0.22568858443106166 1101 16-17 0.2430345841760584 1101 18-19 0.24178371360908102 1101 20-21 0.31577765215796205 1101 22-23 0.17918074210847124 1101 24-25 0.19622457936029036 1101 26-27 0.35643577329638276 1101 28-29 0.26572437531900306 1101 30-31 0.4141380211089327 1101 32-33 0.4015438942461884 1101 34-35 0.4306185664826998 1101 36-37 0.3107683829452341 1101 38-39 0.33869473599904865 1101 40-41 0.4292670736086279 1101 42-43 0.5819601222631618 1101 44-45 0.566308130001353 1101 46-47 0.6505724377191129 1101 48-49 0.6729858217010474 1101 50-51 0.7659016750493493 1101 52-53 0.6443441208080642 1101 54-55 0.5113701802589858 1101 56-57 0.60166968338374 1101 58-59 0.4547878620790371 1101 60-61 0.5544164915865366 1101 62-63 0.48375847991263043 1101 64-65 0.3923378957044008 1101 66-67 0.5722043126940335 1101 68-69 0.6576520604467397 1101 70-71 0.5418616577980977 1101 72-73 0.5648559023429094 1101 74-75 0.7569960671160416 1101 76-77 0.8648654857839091 1101 78-79 0.8933179493028014 1101 80-81 0.7300444324256397 1101 82-83 0.8265288836791207 1101 84-85 0.9724749237959891 1101 86-87 1.1226416740452585 1101 88-89 1.1149262378940001 1101 90-91 1.077116089906717 1101 92-93 1.1838795566734213 1101 94-95 1.3563106721157112 1101 96-97 1.4391066656274027 1101 98-99 1.5728311798303523 1101 100-101 1.305144918114724 1102 1 0.15056466160976356 1102 2 0.1552509934248434 1102 3 0.10089861564321367 1102 4 0.19701108654298594 1102 5 0.2391484335312768 1102 6 0.06822954842840545 1102 7 0.07092512962128694 1102 8 -0.02405585033731228 1102 9 0.181827796061647 1102 10-11 0.10921340119102041 1102 12-13 0.2570410751621992 1102 14-15 0.4162270481615238 1102 16-17 0.3445987538617814 1102 18-19 0.4227243785236823 1102 20-21 0.23603475704515375 1102 22-23 0.27215094827681696 1102 24-25 0.33805565913735336 1102 26-27 0.3238632607678724 1102 28-29 0.376859305031104 1102 30-31 0.38957158081252174 1102 32-33 0.3301197174882802 1102 34-35 0.3448269160608959 1102 36-37 0.46213411705969776 1102 38-39 0.34220864527046757 1102 40-41 0.5362972884034747 1102 42-43 0.7528347477678281 1102 44-45 0.6699437623981552 1102 46-47 0.7352354210658021 1102 48-49 0.6241004998455395 1102 50-51 0.4511178687908597 1102 52-53 0.3750322725724189 1102 54-55 0.5120890558598248 1102 56-57 0.560126931009826 1102 58-59 0.6207781812209419 1102 60-61 0.5815746079511257 1102 62-63 0.7279488640771632 1102 64-65 0.6986930474519113 1102 66-67 0.8406416908575736 1102 68-69 0.7879110240483236 1102 70-71 0.951348095659398 1102 72-73 0.909661022291516 1102 74-75 0.7669892475123703 1102 76-77 0.8980210323301314 1102 78-79 0.9205235049806895 1102 80-81 0.9391928692274085 1102 82-83 0.8201287860156619 1102 84-85 1.038642079859347 1102 86-87 1.1204809995197458 1102 88-89 1.1507351478065821 1102 90-91 1.1417954649123843 1102 92-93 1.3807886506440603 1102 94-95 1.2055718493360317 1102 96-97 1.300784152127438 1102 98-99 1.7892797659769073 1102 100-101 1.771964275686038 1103 1 0.3770077952062749 1103 2 0.33684151208830926 1103 3 0.256395265206919 1103 4 0.25179697716745153 1103 5 0.23597006972736523 1103 6 0.27573617535249184 1103 7 0.26435736434523704 1103 8 0.26241356342166 1103 9 0.3336696827725305 1103 10-11 0.3683048015482555 1103 12-13 0.4031796831099612 1103 14-15 0.3943233575620795 1103 16-17 0.3096523949726091 1103 18-19 0.42715133446174036 1103 20-21 0.2659703934005151 1103 22-23 0.25746576983337377 1103 24-25 0.5560487534842764 1103 26-27 0.3894506110221343 1103 28-29 0.32327081141911407 1103 30-31 0.3557636023372268 1103 32-33 0.4038094067907707 1103 34-35 0.5273901420361611 1103 36-37 0.5480644177457137 1103 38-39 0.4761933891242123 1103 40-41 0.6510305333778206 1103 42-43 0.21319408063850886 1103 44-45 0.37767934528306313 1103 46-47 0.3757688742336427 1103 48-49 0.3119681092608957 1103 50-51 0.35260138965002596 1103 52-53 0.48985084274955426 1103 54-55 0.8195413590714722 1103 56-57 0.5417429050254654 1103 58-59 0.46586245407956284 1103 60-61 0.5252222888121096 1103 62-63 0.6098523840261976 1103 64-65 0.6907179851645182 1103 66-67 0.9298534566342056 1103 68-69 0.8699184985360908 1103 70-71 0.9054054512607124 1103 72-73 0.8467934128761634 1103 74-75 0.775028303701859 1103 76-77 0.8251142017632311 1103 78-79 0.876053431373478 1103 80-81 0.94615896099182 1103 82-83 0.9711245311322152 1103 84-85 1.151023904453485 1103 86-87 1.3392875274665954 1103 88-89 1.4340589057560464 1103 90-91 1.4805429193771715 1103 92-93 1.6364826858435535 1103 94-95 1.504647350763804 1103 96-97 1.5003406032816038 1103 98-99 1.4309018265236695 1103 100-101 1.5008725965998124 1104 1 0.05577588918126963 1104 2 0.19956792360782316 1104 3 0.17882648915538368 1104 4 0.18061812154031998 1104 5 0.24172793255419123 1104 6 0.17250881650443972 1104 7 0.30149112713417026 1104 8 0.07617900487675655 1104 9 0.18457002381080656 1104 10-11 0.06110396789913608 1104 12-13 0.22743830379960883 1104 14-15 0.4440836834430968 1104 16-17 0.31797448667401085 1104 18-19 0.2748455368111138 1104 20-21 0.24615929071010356 1104 22-23 0.40592408737789754 1104 24-25 0.4505042290432044 1104 26-27 0.3609828201922838 1104 28-29 0.32969710925920737 1104 30-31 0.4087895591390449 1104 32-33 0.35234142649520805 1104 34-35 0.3887736206265373 1104 36-37 0.4775212195645864 1104 38-39 0.5553354884042392 1104 40-41 0.6145335648291237 1104 42-43 0.5839072295585552 1104 44-45 0.5933671057984071 1104 46-47 0.6790856608952609 1104 48-49 0.6487835696625694 1104 50-51 0.6093770622532872 1104 52-53 0.4578487586267812 1104 54-55 0.870558410985069 1104 56-57 0.8094172134756334 1104 58-59 0.8999069785206402 1104 60-61 0.9332940963149525 1104 62-63 0.941829837607095 1104 64-65 0.8832268900527325 1104 66-67 0.8695798681537141 1104 68-69 0.7564652965656471 1104 70-71 0.5316223894948919 1104 72-73 0.8361317986283403 1104 74-75 0.7635444082869327 1104 76-77 0.9431727466666047 1104 78-79 1.1529253525557444 1104 80-81 1.051694201613266 1104 82-83 1.155181370844229 1104 84-85 1.1024361818312798 1104 86-87 1.3626865422449193 1104 88-89 1.356251402914058 1104 90-91 1.4437212066071794 1104 92-93 1.46247207576398 1104 94-95 1.4451060472397472 1104 96-97 1.194644695740866 1104 98-99 1.2561850777551982 1104 100-101 0.9814938546521041 1105 1 0.020026030238256 1105 2 0.08633113594560982 1105 3 0.02498259222102206 1105 4 0.07943286669736693 1105 5 -0.015693545723031832 1105 6 -0.10808625590480858 1105 7 0.07065832668278915 1105 8 0.03895763944512254 1105 9 0.25271409001354783 1105 10-11 0.15625330022940176 1105 12-13 0.05357277812723993 1105 14-15 0.16927834208518533 1105 16-17 0.034457277721593016 1105 18-19 0.17564580011749342 1105 20-21 0.22849086866759905 1105 22-23 0.13670610731394817 1105 24-25 0.15744348050078827 1105 26-27 0.17207779856354222 1105 28-29 0.171567148755166 1105 30-31 0.2854739676400797 1105 32-33 0.02048079079838061 1105 34-35 0.47694871936643324 1105 36-37 0.43003730006110885 1105 38-39 0.37642012259645696 1105 40-41 0.3235911160798324 1105 42-43 0.349139315321203 1105 44-45 0.43661047612001624 1105 46-47 0.44311443652060944 1105 48-49 0.4931426067135334 1105 50-51 0.41930408877199454 1105 52-53 0.4858528962984039 1105 54-55 0.7341700340807975 1105 56-57 0.7325430363080514 1105 58-59 0.6140092918552256 1105 60-61 0.43225177921884494 1105 62-63 0.5719919591041887 1105 64-65 0.8703779150668325 1105 66-67 0.935149926457278 1105 68-69 0.8480129738272559 1105 70-71 0.9993726245898706 1105 72-73 0.9788829741032323 1105 74-75 0.9987838290129041 1105 76-77 1.0015455132643112 1105 78-79 0.9295390444872851 1105 80-81 0.9057782715775318 1105 82-83 1.0249805056947068 1105 84-85 1.0556934697756084 1105 86-87 1.0613417989686376 1105 88-89 1.0063411151578059 1105 90-91 1.1721545336713142 1105 92-93 1.4740701573889545 1105 94-95 1.6681220336982747 1105 96-97 1.6497475733034044 1105 98-99 1.933055493403117 1105 100-101 1.735661430352689 1106 1 0.005950695301578435 1106 2 -0.6509958994900593 1106 3 -0.06398298474945818 1106 4 -0.03577802182584833 1106 5 0.04821923416810847 1106 6 -0.06204620340752598 1106 7 0.06312516611970409 1106 8 0.1326183257738478 1106 9 0.16593367922895652 1106 10-11 -0.3964013768923067 1106 12-13 -0.28282872617314325 1106 14-15 -0.10141411576042714 1106 16-17 -0.1543464104125576 1106 18-19 0.03445358543824284 1106 20-21 -0.0364039035887842 1106 22-23 0.035951125734634104 1106 24-25 0.08332187733495999 1106 26-27 0.012464899420955078 1106 28-29 0.05994213126717085 1106 30-31 0.014087819461835238 1106 32-33 0.19919973712190853 1106 34-35 0.21457554894345776 1106 36-37 0.11393685569957057 1106 38-39 0.17304477366938897 1106 40-41 0.2501323910710056 1106 42-43 0.13875044900940736 1106 44-45 0.28888691296671 1106 46-47 0.6044436571224665 1106 48-49 0.4994890821337421 1106 50-51 0.39099852525894363 1106 52-53 0.36384833942745587 1106 54-55 0.48042510559836415 1106 56-57 0.438584054808679 1106 58-59 0.27493129208173883 1106 60-61 0.4198386362436892 1106 62-63 0.8157212649808159 1106 64-65 0.6512034148902899 1106 66-67 0.8490323938296811 1106 68-69 0.8105770131868972 1106 70-71 0.8522761308489066 1106 72-73 0.7723514590768836 1106 74-75 0.6879489356396888 1106 76-77 0.8231788250568783 1106 78-79 0.8838654070516583 1106 80-81 0.8312519463208758 1106 82-83 0.7569495536151365 1106 84-85 0.6397757332879941 1106 86-87 0.8906156975582711 1106 88-89 1.0254771417528765 1106 90-91 1.0025484964835165 1106 92-93 1.2071159659337525 1106 94-95 1.1614732658521945 1106 96-97 1.016629814164677 1106 98-99 1.3257899823873487 1106 100-101 0.7914114819839035 1107 1 0.1803635502511618 1107 2 0.21967139881282094 1107 3 0.15066210024480142 1107 4 0.27016673536304836 1107 5 0.18386772169905896 1107 6 0.20196332646148818 1107 7 0.1822295105781393 1107 8 0.18645897363896324 1107 9 0.14126701717277257 1107 10-11 0.19359986793818962 1107 12-13 0.2533240906016445 1107 14-15 0.218050883755474 1107 16-17 0.3206783133042279 1107 18-19 0.3483773603175635 1107 20-21 0.34291583112400303 1107 22-23 0.33757441927949117 1107 24-25 0.3748767397811932 1107 26-27 0.3951534566624062 1107 28-29 0.37133475080548095 1107 30-31 0.16539264387157004 1107 32-33 0.2857906650738826 1107 34-35 0.3450225468272379 1107 36-37 0.44074808407063415 1107 38-39 0.6515844146049403 1107 40-41 0.6559566417522049 1107 42-43 0.5770103142910799 1107 44-45 0.4975109949042249 1107 46-47 0.44924009275781174 1107 48-49 0.5539694345590931 1107 50-51 0.3894018572552369 1107 52-53 0.515334701617796 1107 54-55 0.6115273684235731 1107 56-57 0.7812124106642315 1107 58-59 0.6828847215897511 1107 60-61 0.8576455215394745 1107 62-63 0.9012601382709065 1107 64-65 0.6353914741995439 1107 66-67 0.9887620235617689 1107 68-69 1.0135414386976258 1107 70-71 1.101204193477713 1107 72-73 1.1533326967150295 1107 74-75 1.087365767131132 1107 76-77 1.1442344413171313 1107 78-79 1.205679159662342 1107 80-81 1.372714310945561 1107 82-83 1.274466220512327 1107 84-85 1.2131066230651726 1107 86-87 1.4876865422449193 1107 88-89 1.4216764321994972 1107 90-91 1.4514144904806656 1107 92-93 1.3955737938428534 1107 94-95 1.1659555201019103 1107 96-97 0.9897319663382902 1107 98-99 1.4657409153186478 1107 100-101 1.3206961193924407 1108 1 0.04229931476418258 1108 2 0.028591676059338766 1108 3 0.039287155967326015 1108 4 0.009669330954139355 1108 5 0.07195891969735868 1108 6 0.1644225231943537 1108 7 0.08547543744142416 1108 8 0.168791338718556 1108 9 0.19544908242924208 1108 10-11 0.1889047959662591 1108 12-13 0.1509878788704313 1108 14-15 0.11391807001969312 1108 16-17 0.09154999332033498 1108 18-19 0.027255647946439865 1108 20-21 -0.027638050758625354 1108 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1.5001312172894679 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6050.0 3 1245.0 4 1235.0 5 1307.0 6 1626.0 7 1840.0 8 2349.0 9 2876.0 10 3689.0 11 4428.0 12 5603.0 13 7052.0 14 7969.0 15 9596.0 16 11794.0 17 14941.0 18 13452.0 19 10914.0 20 11341.0 21 12192.0 22 13414.0 23 14962.0 24 16970.0 25 19373.0 26 22529.0 27 26365.0 28 30621.0 29 36310.0 30 43274.0 31 52591.0 32 65657.0 33 82930.0 34 107558.0 35 142133.0 36 188618.0 37 220587.0 38 133549.0 39 10947.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.75491794634673 18.83056305922627 14.642961704362012 22.77155729006499 2 15.039989620170486 19.86027039161003 40.87986748024478 24.219872507974703 3 19.427024165921488 24.374841964265308 29.287937975535517 26.910195894277688 4 12.449603499612603 16.213500548841754 37.22746818450224 34.1094277670434 5 13.637690604183192 37.56527944467871 33.953420480716204 14.843609470421887 6 32.13965386489042 36.06184532184021 17.490929485455116 14.307571327814255 7 29.038929108649363 32.430384765902815 20.259483170959818 18.271202954488004 8 26.57645241457382 35.15356223707368 19.206924303751943 19.063061044600556 9 26.237361043341355 17.121686304744244 20.034966735902376 36.605985916012024 10-11 25.001501927073793 26.850807225909367 27.09026784427578 21.057423002741064 12-13 26.03002452290168 23.914067388963296 27.787056890625795 22.268851197509235 14-15 22.589118599883623 25.437877242912837 26.151356701772517 25.821647455431023 16-17 22.15524665233722 29.09370360448367 26.48558696627077 22.26546277690834 18-19 22.073993997180605 27.459103674276676 28.917694463419718 21.549207865122998 20-21 22.721993480934717 26.438509207829657 29.121230589641566 21.718266721594055 22-23 22.349322410045552 26.374109832985564 29.08052706804124 22.196040688927642 24-25 22.32632724694778 26.562736051019815 29.13019735983958 21.980739342192823 26-27 22.069639144357364 26.829784121821156 29.09025794036057 22.010318793460907 28-29 21.78666144878126 27.069217681998627 29.184672240517983 21.959448628702127 30-31 22.060933947070684 26.590256612479408 29.142045307968147 22.206764132481766 32-33 21.735892387363336 26.865460988281875 29.11467505681443 22.28397156754036 34-35 22.42756119940892 26.826032642141456 28.8413108447232 21.90509531372643 36-37 22.228666389084385 26.67144452100475 28.892704621745008 22.207184468165856 38-39 21.912267954938013 27.00834301108864 28.98606845366173 22.093320580311616 40-41 22.196170263875555 26.809737111833392 29.003394696256844 21.99069792803421 42-43 22.01205117160484 26.876072937849838 28.680321909911356 22.431553980633968 44-45 22.14017191263398 26.994019645157998 28.52527198252945 22.34053645967857 46-47 22.268496033971577 27.194875963502074 28.168198222564833 22.368429779961517 48-49 22.296617817380636 27.32064202620682 28.01621832163292 22.366521834779626 50-51 22.250808859326842 27.36142882572311 28.04537056795026 22.342391746999784 52-53 22.53172749497546 27.500983162580628 27.695293682733528 22.271995659710385 54-55 22.18481474084059 27.311658285605628 27.893566543757544 22.60996042979624 56-57 22.28622735606029 27.534861203628925 27.81037597929697 22.36853546101381 58-59 22.57680550121682 27.246215997766033 27.79343470178533 22.383543799231816 60-61 22.364277398723825 27.253754107782314 28.31451981314196 22.067448680351905 62-63 22.250902469882668 27.40093094954053 28.155586618318797 22.192579962258005 64-65 22.088006171425036 27.50439684561785 27.945481324734402 22.462115658222707 66-67 22.064192112870952 27.576878342686804 27.7430340773991 22.61589546704314 68-69 22.008156859277598 27.454168324564893 27.95547990627349 22.58219490988402 70-71 22.142646614546873 27.478207688283362 27.84394273208904 22.53520296508072 72-73 22.266786284699517 27.31844985345563 27.88738465732934 22.52737920451552 74-75 22.000086952933408 27.77287746415771 27.68854048764384 22.53849509526504 76-77 22.129113084490953 27.50554552489038 27.60610191974649 22.759239470872178 78-79 22.21732560371806 27.521997014618492 27.639245643984967 22.621431737678485 80-81 22.172644927002246 27.70875029844631 27.6353914583929 22.483213316158547 82-83 22.218144948552666 27.517839300398787 27.66915282112951 22.594862929919035 84-85 22.220384363745673 27.456684663762772 27.578437671301508 22.744493301190047 86-87 22.34114781824498 27.452901816879717 27.643029682566407 22.5629206823089 88-89 22.124076390411222 27.45880484781051 27.41083517702172 23.006283584756552 90-91 22.189100806998308 27.310872666395507 27.738945773722158 22.76108075288403 92-93 22.43021300489736 27.20115868297449 27.835840472551652 22.532787839576496 94-95 22.142355026917414 27.25235315529867 27.646793121285317 22.9584986964986 96-97 22.096212068456676 27.173831744021058 27.69242616339243 23.03753002412984 98-99 22.126709420211093 27.201202650695187 27.435063678954165 23.237024250139555 100-101 22.515991722581973 26.309574992088415 28.121190418560747 23.053242866768862 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1973.0 1 1102.0 2 248.0 3 267.0 4 230.0 5 222.5 6 351.5 7 666.5 8 1091.0 9 1336.5 10 1294.0 11 1265.0 12 1367.0 13 1451.0 14 1571.0 15 1744.0 16 1813.0 17 1854.0 18 1983.5 19 2143.0 20 2388.5 21 2777.0 22 3287.5 23 3843.0 24 4580.0 25 5700.0 26 7092.5 27 8784.0 28 10493.0 29 12599.0 30 15407.5 31 17842.0 32 20357.0 33 23255.0 34 26282.5 35 28873.0 36 32003.5 37 35414.5 38 37692.0 39 39316.0 40 40690.5 41 42968.5 42 45306.5 43 47766.0 44 50081.0 45 51763.5 46 52254.0 47 52973.0 48 56540.0 49 56965.5 50 53517.5 51 50369.5 52 47603.0 53 46160.5 54 44445.0 55 40641.0 56 36376.0 57 32490.5 58 28959.5 59 25560.5 60 21550.0 61 17584.0 62 13953.0 63 10627.5 64 8093.5 65 6279.0 66 4655.0 67 3552.5 68 2641.0 69 1728.0 70 1228.5 71 924.5 72 720.5 73 554.0 74 425.0 75 316.0 76 211.0 77 141.0 78 87.5 79 64.5 80 46.0 81 26.5 82 15.5 83 13.5 84 9.5 85 6.5 86 5.5 87 5.5 88 5.0 89 3.5 90 1.0 91 0.5 92 0.5 93 1.5 94 1.5 95 0.0 96 1.0 97 2.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28575861706359856 2 0.2509028310398863 3 0.24943212276884366 4 0.24972626442305218 5 0.2509763664534385 6 0.2220769489274492 7 0.22340058637138768 8 0.22391533426625262 9 0.2331807963738217 10-11 0.24255656160171896 12-13 0.23516625253972934 14-15 0.22704058934221835 16-17 0.22130482708515178 18-19 0.21031128275910754 20-21 0.2166353283245912 22-23 0.2140248211434904 24-25 0.2151646200535485 26-27 0.20961269633036225 28-29 0.2218931103935689 30-31 0.2334014026144781 32-33 0.22498159776275858 34-35 0.22457715298822184 36-37 0.21575290336196556 38-39 0.2077375432847828 40-41 0.2038401663665196 42-43 0.21512785234677245 44-45 0.2104951212929879 46-47 0.21987088652088518 48-49 0.2227019999426424 50-51 0.23490887859229687 52-53 0.24248302618816686 54-55 0.22501836546953463 56-57 0.20538441005111446 58-59 0.19575127087578473 60-61 0.19920743531273508 62-63 0.20442844967493667 64-65 0.19604541252999327 66-67 0.2006413758770017 68-69 0.20214885185482048 70-71 0.20288420599034185 72-73 0.20527410693078627 74-75 0.20832582659319987 76-77 0.21612058042972623 78-79 0.21645148979071085 80-81 0.21038481817265967 82-83 0.20391370178007173 84-85 0.19361874388277278 86-87 0.19446440113862234 88-89 0.2045387527952649 90-91 0.20303127681744615 92-93 0.20817875576609562 94-95 0.20406077260717603 96-97 0.20998037339812295 98-99 0.21225997121823914 100-101 0.19872945512464624 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1359889.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.909642367932996 #Duplication Level Percentage of deduplicated Percentage of total 1 82.01899592916145 51.59785701280498 2 10.620870026070065 13.36310269952734 3 3.15844324135783 5.960896042597085 4 1.340810059339204 3.3739952526542534 5 0.717011701375978 2.2553474853592976 6 0.437057374841132 1.6497073887313953 7 0.3009920152817883 1.3254710025886507 8 0.21808324474970298 1.0975631146913774 9 0.16312599839816744 0.9235978398126652 >10 0.9274297735660775 10.613745224617643 >50 0.06540675781012048 2.825460594744358 >100 0.0301384843179437 3.413881624967987 >500 9.345107031820506E-4 0.4009459867406048 >1k 7.008830273865379E-4 1.1984287301623502 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4954 0.3642944387372793 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3644 0.26796304698398177 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2572 0.18913308365609252 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2147 0.15788053289643492 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1873 0.1377318295831498 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 7.353541355213551E-5 3 0.0 0.0 0.0 0.0 7.353541355213551E-5 4 0.0 0.0 0.0 0.0 7.353541355213551E-5 5 7.353541355213551E-5 0.0 0.0 0.0 7.353541355213551E-5 6 7.353541355213551E-5 0.0 0.0 0.0 7.353541355213551E-5 7 7.353541355213551E-5 0.0 0.0 0.0 7.353541355213551E-5 8 7.353541355213551E-5 0.0 0.0 0.0 1.4707082710427102E-4 9 7.353541355213551E-5 0.0 0.0 0.0 1.4707082710427102E-4 10-11 7.353541355213551E-5 0.0 0.0 0.0 1.4707082710427102E-4 12-13 7.353541355213551E-5 0.0 0.0 0.0 1.4707082710427102E-4 14-15 7.353541355213551E-5 0.0 0.0 0.0 1.4707082710427102E-4 16-17 7.353541355213551E-5 0.0 0.0 3.6767706776067755E-5 1.4707082710427102E-4 18-19 7.353541355213551E-5 0.0 0.0 7.353541355213551E-5 1.4707082710427102E-4 20-21 7.353541355213551E-5 0.0 0.0 1.4707082710427102E-4 1.4707082710427102E-4 22-23 7.353541355213551E-5 0.0 0.0 3.3090936098460977E-4 1.4707082710427102E-4 24-25 7.353541355213551E-5 0.0 0.0 5.882833084170841E-4 1.4707082710427102E-4 26-27 7.353541355213551E-5 0.0 0.0 7.721218422974227E-4 1.4707082710427102E-4 28-29 7.353541355213551E-5 0.0 0.0 0.0014707082710427102 1.4707082710427102E-4 30-31 7.353541355213551E-5 0.0 0.0 0.003309093609846098 1.4707082710427102E-4 32-33 7.353541355213551E-5 0.0 0.0 0.005478388309634095 1.4707082710427102E-4 34-35 7.353541355213551E-5 0.0 0.0 0.007610915302646026 1.4707082710427102E-4 36-37 7.353541355213551E-5 0.0 0.0 0.010552331844731446 1.4707082710427102E-4 38-39 7.353541355213551E-5 0.0 0.0 0.015258598312068116 1.4707082710427102E-4 40-41 7.353541355213551E-5 0.0 0.0 0.023751938577339766 1.4707082710427102E-4 42-43 1.1030312032820326E-4 0.0 0.0 0.03228204654938749 1.4707082710427102E-4 44-45 1.4707082710427102E-4 0.0 0.0 0.04283437839411893 1.4707082710427102E-4 46-47 1.4707082710427102E-4 0.0 0.0 0.06364490042937328 1.4707082710427102E-4 48-49 1.4707082710427102E-4 0.0 0.0 0.0790138018617696 1.4707082710427102E-4 50-51 1.4707082710427102E-4 0.0 0.0 0.09879482810729405 1.4707082710427102E-4 52-53 1.4707082710427102E-4 0.0 0.0 0.1226938375117381 1.4707082710427102E-4 54-55 1.4707082710427102E-4 0.0 0.0 0.15534356112888625 1.4707082710427102E-4 56-57 2.2060624065640651E-4 0.0 0.0 0.23751938577339768 1.4707082710427102E-4 58-59 2.2060624065640651E-4 0.0 0.0 0.29903175920975905 2.2060624065640651E-4 60-61 2.2060624065640651E-4 0.0 0.0 0.3489990727184351 2.2060624065640651E-4 62-63 2.2060624065640651E-4 0.0 0.0 0.4113938711174221 2.2060624065640651E-4 64-65 2.2060624065640651E-4 0.0 0.0 0.47231796124536635 2.2060624065640651E-4 66-67 2.2060624065640651E-4 0.0 0.0 0.572509962210151 2.2060624065640651E-4 68-69 2.2060624065640651E-4 0.0 0.0 0.7113080552898068 3.3090936098460977E-4 70-71 2.2060624065640651E-4 0.0 0.0 0.78682892500785 4.4121248131281303E-4 72-73 2.2060624065640651E-4 0.0 0.0 0.8743360671348912 4.4121248131281303E-4 74-75 2.2060624065640651E-4 0.0 0.0 1.0347903395056508 4.4121248131281303E-4 76-77 2.2060624065640651E-4 0.0 0.0 1.214988870415159 4.4121248131281303E-4 78-79 2.2060624065640651E-4 0.0 0.0 1.3929445712113266 4.4121248131281303E-4 80-81 2.2060624065640651E-4 0.0 0.0 1.5675544106908725 4.7798018808888076E-4 82-83 2.2060624065640651E-4 0.0 0.0 1.8056253120659114 5.882833084170841E-4 84-85 2.2060624065640651E-4 0.0 0.0 2.156756911777358 5.882833084170841E-4 86-87 2.2060624065640651E-4 0.0 0.0 2.4265215763933674 5.882833084170841E-4 88-89 2.2060624065640651E-4 0.0 0.0 2.8018095594566907 5.882833084170841E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7550 0.0 30.91193 1 TATCACG 80 4.483781E-4 29.699114 2 GGTATCA 5100 0.0 27.867155 1 ACCGTAT 70 0.00838514 27.14747 8 GTACATG 6055 0.0 26.925982 1 TACATGG 6085 0.0 26.23869 2 TCAACGC 8770 0.0 26.226557 4 ATCAACG 8785 0.0 26.125753 3 CAACGCA 8840 0.0 26.0208 5 AACGCAG 8990 0.0 25.630049 6 TATCAAC 8985 0.0 25.491327 2 ACATGGG 6160 0.0 24.684977 3 ACGCAGA 10030 0.0 22.688301 7 CGCAGAG 10060 0.0 22.667868 8 GAGTACT 4695 0.0 22.66208 12-13 GTACCTG 1105 0.0 22.368284 1 AGTACTT 4940 0.0 22.06699 12-13 TACCTGG 1120 0.0 21.637926 2 AGAGTAC 7850 0.0 21.483355 10-11 GCAGAGT 10550 0.0 21.209764 9 >>END_MODULE