FastQCFastQC Report
Thu 26 May 2016
SRR937203_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937203_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359889
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65460.481362817112279No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51500.3787073797934979No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36860.2710515343531715No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20750.15258598312068117No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13860.1019200831832598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA79450.030.313641
GGTATCA54050.028.2998561
ATCAACG96450.024.9186463
TCAACGC96600.024.830784
CAACGCA98250.024.4137745
TATCAAC99400.024.1791082
AACGCAG100150.024.0454646
GTACATG54150.023.685871
TACATGG53850.023.1977672
CCTACAC5950.022.3520413
ACATGGG54300.022.2182583
GAGTACT60000.022.20699112-13
TACCTGG10550.022.0607222
AGTACTT60950.022.05569612-13
GTACTTT60250.021.7986814-15
AGAGTAC87750.021.68020810-11
ACGCAGA111100.021.5061177
CGCAGAG111900.021.2250148
GTACCTG12350.020.0014021
GCAGAGT118850.019.9838379
Produced by FastQC (version 0.11.5)