##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937203_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1359889 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29802726546064 33.0 31.0 34.0 31.0 34.0 2 32.49535292954057 34.0 31.0 34.0 31.0 34.0 3 32.56215102850306 34.0 31.0 34.0 31.0 34.0 4 36.16447077665898 37.0 35.0 37.0 35.0 37.0 5 36.09757119882579 37.0 35.0 37.0 35.0 37.0 6 36.139230481311344 37.0 35.0 37.0 35.0 37.0 7 36.086073201562776 37.0 35.0 37.0 35.0 37.0 8 36.06224625686361 37.0 35.0 37.0 35.0 37.0 9 37.777699503415356 39.0 37.0 39.0 35.0 39.0 10-11 37.69596194983561 39.0 37.0 39.0 35.0 39.0 12-13 37.58954922056138 39.0 37.0 39.0 35.0 39.0 14-15 38.843713347192306 40.0 38.0 41.0 35.0 41.0 16-17 38.797470602380045 40.0 38.0 41.0 35.0 41.0 18-19 38.771847187527804 40.0 38.0 41.0 35.0 41.0 20-21 38.66409170160212 40.0 38.0 41.0 34.0 41.0 22-23 38.616997416700926 40.0 38.0 41.0 34.0 41.0 24-25 38.64748814057618 40.0 38.0 41.0 34.0 41.0 26-27 38.50403930026642 40.0 38.0 41.0 34.0 41.0 28-29 38.357573301938615 40.0 38.0 41.0 34.0 41.0 30-31 38.415236096475525 40.0 38.0 41.0 34.0 41.0 32-33 38.324026813953196 40.0 38.0 41.0 34.0 41.0 34-35 38.1967660595828 40.0 38.0 41.0 34.0 41.0 36-37 38.03558636035735 40.0 37.5 41.0 33.5 41.0 38-39 37.92362097200581 40.0 37.0 41.0 33.0 41.0 40-41 37.74631275052596 40.0 37.0 41.0 33.0 41.0 42-43 37.54911724412801 40.0 37.0 41.0 33.0 41.0 44-45 37.25347987960782 40.0 37.0 41.0 32.5 41.0 46-47 36.8986608465838 40.0 36.0 41.0 31.0 41.0 48-49 36.569478464786464 39.0 36.0 41.0 30.5 41.0 50-51 36.320296730100765 39.0 36.0 41.0 30.0 41.0 52-53 36.07777877459116 39.0 35.0 40.0 30.0 41.0 54-55 35.862556796915044 39.0 35.0 40.0 29.0 41.0 56-57 35.55684434538407 38.5 35.0 40.0 28.0 41.0 58-59 35.282890368257995 38.0 35.0 40.0 28.0 41.0 60-61 34.92221497489869 38.0 34.0 40.0 27.5 41.0 62-63 34.549951871071826 37.5 34.0 40.0 26.5 41.0 64-65 34.12727766751551 37.0 33.5 39.5 26.0 41.0 66-67 33.682711971344716 36.0 33.0 39.0 25.5 40.0 68-69 33.220559545668806 36.0 32.5 39.0 25.0 40.0 70-71 32.76807592384378 35.0 32.0 38.0 23.5 40.0 72-73 32.284025755043245 35.0 31.5 37.5 22.0 39.5 74-75 31.79817764538135 35.0 31.0 37.0 21.5 39.0 76-77 30.491325394940326 33.5 29.5 35.5 20.0 38.0 78-79 31.139522784580212 34.0 31.0 36.0 20.0 38.0 80-81 30.9512908774172 34.0 31.0 36.0 20.0 37.0 82-83 30.546118469963357 34.0 30.5 35.0 18.0 37.0 84-85 30.107545542319997 34.0 30.0 35.0 15.5 36.5 86-87 29.711464318043603 34.0 30.0 35.0 9.0 36.0 88-89 29.25658969224694 34.0 29.0 35.0 4.5 36.0 90-91 28.69627925514509 34.0 29.0 35.0 2.0 35.0 92-93 28.17834323242559 33.0 28.5 35.0 2.0 35.0 94-95 27.670152122710014 33.0 27.0 35.0 2.0 35.0 96-97 26.96320618815212 33.0 25.5 34.5 2.0 35.0 98-99 25.950357345342155 32.0 24.5 34.0 2.0 35.0 100-101 22.92528324002915 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14112146090144506 1101 2 0.2527659998954803 1101 3 0.18318162481349276 1101 4 0.08878686015326309 1101 5 0.11020117331432999 1101 6 0.12462469511838492 1101 7 0.13701252252067064 1101 8 0.16570170195255685 1101 9 0.2550307931068687 1101 10-11 0.1974676208506736 1101 12-13 0.18600781586410164 1101 14-15 0.22191098824497857 1101 16-17 0.1860023677424465 1101 18-19 0.0769949137317738 1101 20-21 0.09110221104230476 1101 22-23 0.13863901368547005 1101 24-25 0.049016279647176475 1101 26-27 0.09373320194873003 1101 28-29 0.05090354502878114 1101 30-31 0.27975589519410704 1101 32-33 0.4156048736773812 1101 34-35 0.38208705070370996 1101 36-37 0.3477926882423148 1101 38-39 0.2459432456375268 1101 40-41 0.35403480261701503 1101 42-43 0.40366648239091063 1101 44-45 0.23459690566288316 1101 46-47 0.28559098252424775 1101 48-49 0.25983547465627055 1101 50-51 0.44940917851358364 1101 52-53 0.4053807356345942 1101 54-55 0.36489145389636946 1101 56-57 0.46066004098630486 1101 58-59 0.42487450437028684 1101 60-61 0.5997604724680059 1101 62-63 0.4909448248540613 1101 64-65 0.5570536490664679 1101 66-67 0.46524718440473833 1101 68-69 0.37275396855302034 1101 70-71 0.44194895967287806 1101 72-73 0.6010154192623034 1101 74-75 0.7498051050848069 1101 76-77 0.6233437423229979 1101 78-79 0.7494651865030342 1101 80-81 0.6119833732829356 1101 82-83 0.5618114133650352 1101 84-85 0.8313403528844816 1101 86-87 0.8632175477264781 1101 88-89 0.9677444836583682 1101 90-91 1.1121548078532193 1101 92-93 1.1381544522571794 1101 94-95 1.0824043936706822 1101 96-97 1.4418824863601856 1101 98-99 1.7938958440064532 1101 100-101 2.261188557620546 1102 1 0.15581631439977883 1102 2 0.1487145853944014 1102 3 0.2022085133515077 1102 4 0.07061549834381964 1102 5 0.052058061373095654 1102 6 0.04233319170987926 1102 7 0.1369694547481899 1102 8 0.1521321154420292 1102 9 0.15991383658005986 1102 10-11 0.0679842009716296 1102 12-13 0.17970615882346408 1102 14-15 0.18997510158640551 1102 16-17 0.27188015370921903 1102 18-19 0.3092637822733906 1102 20-21 0.18500614594714904 1102 22-23 0.13863820414086803 1102 24-25 0.11577375562961123 1102 26-27 0.05826867230709354 1102 28-29 0.05431399450089458 1102 30-31 0.18472604566579065 1102 32-33 0.19380228339373673 1102 34-35 0.18404216566551668 1102 36-37 0.09569969164498815 1102 38-39 0.012772382507556301 1102 40-41 0.12317907512137793 1102 42-43 0.20428892503952767 1102 44-45 0.0826628712423414 1102 46-47 0.10526443813983377 1102 48-49 0.4049996114421077 1102 50-51 0.39157693169703833 1102 52-53 0.23778185138134234 1102 54-55 0.33792228524038137 1102 56-57 0.29005175565356467 1102 58-59 0.26574572331130497 1102 60-61 0.27134231613133863 1102 62-63 0.19397699032757032 1102 64-65 0.2592824224833521 1102 66-67 0.2559023520840853 1102 68-69 0.11966037483560399 1102 70-71 0.1528646323267253 1102 72-73 0.45544570063410106 1102 74-75 0.3413822469131311 1102 76-77 0.3373497705158748 1102 78-79 0.40780530921704283 1102 80-81 0.38313029720776726 1102 82-83 0.2949940958461035 1102 84-85 0.5402014013548353 1102 86-87 0.6313296508715212 1102 88-89 0.666593246479934 1102 90-91 0.9271894110274133 1102 92-93 0.8379068287562923 1102 94-95 0.8565278511644117 1102 96-97 1.0569131065749673 1102 98-99 1.2748402975897584 1102 100-101 2.2581176311518085 1103 1 0.16560453724645186 1103 2 0.21862721238674254 1103 3 0.2571309243004407 1103 4 0.10414261469326647 1103 5 0.17176647671342948 1103 6 0.08144768675532532 1103 7 0.15360360303201048 1103 8 0.1612235157992572 1103 9 0.21981070685162507 1103 10-11 0.2063034651270783 1103 12-13 0.2720529147592572 1103 14-15 0.3424163978468826 1103 16-17 0.3377604538263981 1103 18-19 0.3222445897911115 1103 20-21 0.3495963289981532 1103 22-23 0.2650851773715104 1103 24-25 0.1893404344314149 1103 26-27 0.11383599958043789 1103 28-29 0.2195889123042818 1103 30-31 0.2530295815421866 1103 32-33 0.2869070115476404 1103 34-35 0.2749540361077294 1103 36-37 0.2541919668699677 1103 38-39 0.20160274462771355 1103 40-41 0.2903347026029195 1103 42-43 0.23293472874605214 1103 44-45 0.30130088191368287 1103 46-47 0.4954665451035041 1103 48-49 0.5423618112396014 1103 50-51 0.3820638826293603 1103 52-53 0.35865851095653056 1103 54-55 0.4407875055500554 1103 56-57 0.5294387225132553 1103 58-59 0.4959993382557286 1103 60-61 0.5301258347151716 1103 62-63 0.48071375638861724 1103 64-65 0.5423402502174568 1103 66-67 0.5778044783881384 1103 68-69 0.7541115631312607 1103 70-71 0.6119256000282931 1103 72-73 0.6470811287959677 1103 74-75 0.5426338254293874 1103 76-77 0.542439561070946 1103 78-79 0.4775871042317732 1103 80-81 0.8051231526441178 1103 82-83 0.8122546733270752 1103 84-85 0.7631213151194949 1103 86-87 0.9053835365812581 1103 88-89 1.1299083865839172 1103 90-91 1.2384557508037481 1103 92-93 1.0574158675394436 1103 94-95 0.9973905174063482 1103 96-97 1.1713576679465412 1103 98-99 1.4460175862961115 1103 100-101 2.2190486428811766 1104 1 0.20288115717824695 1104 2 0.1942164507346078 1104 3 0.26219003760093074 1104 4 0.15384022742536274 1104 5 0.06207795833525154 1104 6 0.08070839156775378 1104 7 0.08885824494182515 1104 8 0.12725345138091626 1104 9 0.1982908129524219 1104 10-11 0.07346261632071105 1104 12-13 0.09492502540722114 1104 14-15 0.10971253274646386 1104 16-17 0.18661740721783104 1104 18-19 0.12220480199270867 1104 20-21 0.107450629869696 1104 22-23 0.08979169499182404 1104 24-25 0.006380790626948851 1104 26-27 0.1503958707437576 1104 28-29 0.03556466069382225 1104 30-31 0.10218740647587055 1104 32-33 0.32411637115355063 1104 34-35 0.23409101981367542 1104 36-37 0.30851955307686296 1104 38-39 0.272958561224911 1104 40-41 0.2946736567522237 1104 42-43 0.34942980263767964 1104 44-45 0.29641096148927915 1104 46-47 0.3589974280137014 1104 48-49 0.4423879575071368 1104 50-51 0.47434864958653833 1104 52-53 0.47848552876631345 1104 54-55 0.56008572457241 1104 56-57 0.5550192454386078 1104 58-59 0.5217668638335979 1104 60-61 0.5926944213009975 1104 62-63 0.6063170151987762 1104 64-65 0.43708522937622973 1104 66-67 0.650960977061878 1104 68-69 0.7434256972729827 1104 70-71 0.7532954370877789 1104 72-73 0.8542639632029463 1104 74-75 0.7910978269451086 1104 76-77 0.8483215239356667 1104 78-79 0.8861918787675798 1104 80-81 0.8256422886804984 1104 82-83 0.7385515660894839 1104 84-85 0.8069612165973297 1104 86-87 1.0466019905410917 1104 88-89 1.033743361953377 1104 90-91 1.1868710596328498 1104 92-93 1.1923919948603974 1104 94-95 1.3117923741702704 1104 96-97 1.4073222378593861 1104 98-99 1.502738503310134 1104 100-101 2.026046748224111 1105 1 0.07324151076109331 1105 2 0.05602950320780309 1105 3 0.012419866451786277 1105 4 0.0406361649841358 1105 5 -0.04334728146162092 1105 6 -0.0355549148080172 1105 7 -0.006869797187192717 1105 8 0.07111577976158401 1105 9 0.11000757052247678 1105 10-11 0.03307818901208037 1105 12-13 0.04864592064890161 1105 14-15 0.12161849475511843 1105 16-17 0.14167890806417205 1105 18-19 0.2346676569861188 1105 20-21 0.19718732349391388 1105 22-23 0.15950882871384664 1105 24-25 0.09538379984266498 1105 26-27 0.14281979400875855 1105 28-29 0.1520626858960057 1105 30-31 0.18920170025055683 1105 32-33 0.16837553985770626 1105 34-35 0.22126893969331007 1105 36-37 0.2929376459863988 1105 38-39 0.1835333214280297 1105 40-41 0.2476144128176756 1105 42-43 0.20491146522936532 1105 44-45 0.3215616103514165 1105 46-47 0.2722710785685223 1105 48-49 0.46596196540632207 1105 50-51 0.349394540126319 1105 52-53 0.35476294084078575 1105 54-55 0.49354745110994 1105 56-57 0.4599522903887703 1105 58-59 0.5262357372584674 1105 60-61 0.5613528753164161 1105 62-63 0.6911437972187073 1105 64-65 0.7217577890903542 1105 66-67 0.7080316936313764 1105 68-69 0.7248484920820815 1105 70-71 0.7006858828278624 1105 72-73 0.8298838616419104 1105 74-75 0.7449859217354096 1105 76-77 0.7002624680770992 1105 78-79 0.7717760427698401 1105 80-81 0.8284258132244133 1105 82-83 0.7228838963509112 1105 84-85 0.6710541261327805 1105 86-87 0.8685619303567762 1105 88-89 1.1970123106086312 1105 90-91 1.2149829648425552 1105 92-93 1.282987067269648 1105 94-95 1.3447842869030175 1105 96-97 1.7167788863453772 1105 98-99 1.9384123795561372 1105 100-101 2.425933150330561 1106 1 -0.08550115261005686 1106 2 0.06476350585931812 1106 3 0.06333990136114664 1106 4 0.04191861769994176 1106 5 0.01784236831219488 1106 6 -0.01772715653536494 1106 7 -0.04193571858782974 1106 8 -0.09394587790015407 1106 9 -0.08584794202557333 1106 10-11 -0.19243279344662767 1106 12-13 -0.3083830860997949 1106 14-15 -0.1467322377418938 1106 16-17 -0.1249355166974837 1106 18-19 -0.06155227199597846 1106 20-21 0.03177257495458008 1106 22-23 -0.03709616918759906 1106 24-25 0.06486475625260368 1106 26-27 0.024307838884617183 1106 28-29 -0.0054466576880685125 1106 30-31 -0.03989182219735454 1106 32-33 -0.006739214677516259 1106 34-35 -0.3454310929509461 1106 36-37 0.06853590185070857 1106 38-39 0.08498182678768273 1106 40-41 -0.08291112631212627 1106 42-43 -0.13321052433779101 1106 44-45 -0.1189342491583858 1106 46-47 -0.15104491111855367 1106 48-49 -0.02959192092506413 1106 50-51 -0.03495820432249275 1106 52-53 -0.18810512308864702 1106 54-55 -0.05587361309265049 1106 56-57 -0.0943096164352113 1106 58-59 -0.025845167187149798 1106 60-61 -0.07895911045335424 1106 62-63 -0.06419503678194616 1106 64-65 -0.24434382113729214 1106 66-67 -0.1024900814164269 1106 68-69 -0.04264116502003645 1106 70-71 -0.05343795213879332 1106 72-73 0.06373744884536592 1106 74-75 0.10658179257076483 1106 76-77 -0.0696705112770779 1106 78-79 -0.10504937044641949 1106 80-81 -0.18098106072214293 1106 82-83 -0.15863246241606888 1106 84-85 -0.03143932836179886 1106 86-87 0.2133684965146827 1106 88-89 0.20677060990977836 1106 90-91 0.017215431881854926 1106 92-93 0.1078598213118731 1106 94-95 -0.12718079707265062 1106 96-97 0.050091678228280756 1106 98-99 0.15224887849353408 1106 100-101 0.777618326509586 1107 1 -0.0060809669920018905 1107 2 0.07842379161706958 1107 3 0.11905298176336743 1107 4 0.09943843125199692 1107 5 0.0408682746179565 1107 6 0.0050930069523715815 1107 7 0.06232056434831179 1107 8 0.044894216706964585 1107 9 0.0012521561777205648 1107 10-11 0.0029952207720995716 1107 12-13 0.024575357308819434 1107 14-15 0.04349601576091544 1107 16-17 0.11006859815886827 1107 18-19 0.1214853564636087 1107 20-21 0.11537331632030146 1107 22-23 0.05372199565562141 1107 24-25 -0.12959851433205927 1107 26-27 -0.021424121446791844 1107 28-29 0.08976126358331982 1107 30-31 0.03170322061097153 1107 32-33 0.05954022902156453 1107 34-35 0.047996720711765306 1107 36-37 0.1865830048535102 1107 38-39 0.21000776075634064 1107 40-41 0.2151834185640169 1107 42-43 0.18019630009960963 1107 44-45 0.1315126795681536 1107 46-47 0.11703452289851413 1107 48-49 0.19969721170470933 1107 50-51 0.2950005433780305 1107 52-53 0.29107768026963043 1107 54-55 0.32482235089025124 1107 56-57 0.30781385691850005 1107 58-59 0.3349162195540245 1107 60-61 0.42362218389374107 1107 62-63 0.3823291198453944 1107 64-65 0.45946691621730196 1107 66-67 0.46536355418019326 1107 68-69 0.5072825891121084 1107 70-71 0.4908296034290558 1107 72-73 0.49114135484682464 1107 74-75 0.6142124306155559 1107 76-77 0.46706322639564135 1107 78-79 0.5425157756828476 1107 80-81 0.5402166736863556 1107 82-83 0.5526368843245493 1107 84-85 0.5093063942623033 1107 86-87 0.6950158913610807 1107 88-89 0.9280504685523603 1107 90-91 0.8438529026629169 1107 92-93 0.9910212412485286 1107 94-95 1.2284534440648436 1107 96-97 1.5861822534782881 1107 98-99 1.8002488508306307 1107 100-101 2.7178733784466793 1108 1 -0.03184274781195029 1108 2 0.027768422841816687 1108 3 0.03768012258677089 1108 4 -0.0246304633569423 1108 5 -0.06853481133133954 1108 6 -0.05970869276433888 1108 7 -0.08380725952537915 1108 8 0.06539291531641567 1108 9 -0.016305831196632425 1108 10-11 -0.028701907540693128 1108 12-13 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-0.01536371151642868 2316 76-77 -0.09079935518521509 2316 78-79 -0.17451141793571523 2316 80-81 -0.28099507395686274 2316 82-83 -0.242723159185239 2316 84-85 -0.30903878340267 2316 86-87 -0.21291449297539344 2316 88-89 -0.22111378090376732 2316 90-91 0.3259040266658175 2316 92-93 0.3844799069483109 2316 94-95 0.49126490164280057 2316 96-97 0.586355204892353 2316 98-99 0.84379344836508 2316 100-101 1.640684110861475 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 8.0 5 21.0 6 29.0 7 103.0 8 249.0 9 514.0 10 960.0 11 1492.0 12 2390.0 13 3442.0 14 4796.0 15 6456.0 16 9090.0 17 12890.0 18 11903.0 19 7253.0 20 6519.0 21 6701.0 22 7022.0 23 7697.0 24 8661.0 25 10671.0 26 12971.0 27 16151.0 28 20173.0 29 25147.0 30 31529.0 31 40052.0 32 51789.0 33 68694.0 34 95918.0 35 139339.0 36 213182.0 37 306264.0 38 214454.0 39 15354.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.312221868280055 19.267001301411838 15.073129928573161 22.347646901734947 2 15.767904586330209 20.28577332414631 40.01701609469596 23.92930599482752 3 19.85698832772381 24.29499760642229 29.705806871001972 26.142207194851935 4 13.051285803473666 16.80343027997138 36.922057609113686 33.223226307441266 5 13.752410142674561 37.53432293191007 33.955398940199046 14.757867985216322 6 32.40242402137233 36.24619362315601 17.074114137256792 14.27726821821487 7 29.153776521466092 32.60119024420376 20.08811013251817 18.156923101811987 8 27.02080831597285 35.020064137587696 19.007874907437298 18.951252639002153 9 26.35634231911575 16.88880489510541 20.039429688746655 36.715423097032186 10-11 25.201689255520122 26.943890273397315 26.80443036159569 21.049990109486878 12-13 26.281574456647434 23.880046020746104 27.760224411806988 22.078155110799468 14-15 22.79634587822977 25.30662429065902 26.345569381030366 25.551460450080853 16-17 22.164051624801733 28.971408695856795 26.74637415259628 22.118165526745198 18-19 21.991427241488093 27.072246337752564 29.51020267095329 21.42612374980605 20-21 22.570356107186964 26.045060411336102 29.90108358387526 21.483499897601675 22-23 22.296342324322733 25.961261287760685 29.834838368091305 21.90755801982528 24-25 22.312491060848004 26.129201033761223 29.91815137785193 21.64015652753884 26-27 22.17928476408216 26.426636879264688 29.787573564612213 21.60650479204094 28-29 21.749384209963168 26.70361223042295 29.8822985229496 21.66470503666428 30-31 22.054033980814555 26.298400232372593 29.842854359008296 21.804711427804556 32-33 21.882635058146395 26.35267722710682 29.845689327749042 21.91899838699774 34-35 22.404034446929124 26.327148759935554 29.589326775935387 21.679490017199935 36-37 22.274179096300912 26.157034198024327 29.708943049375 21.859843656299763 38-39 22.028174379066513 26.460598225736238 29.853745308437162 21.657482086760087 40-41 22.194752659959747 26.322994009069856 29.742758416311922 21.739494914658476 42-43 22.026671294878238 26.395109784585923 29.41439273292339 22.163826187612447 44-45 22.185487197852176 26.496243443398686 29.193265038543586 22.125004320205548 46-47 22.300901029422253 26.8744728430041 28.827352820708164 21.997273306865488 48-49 22.453303174009054 26.94337552550245 28.639543374496007 21.96377792599249 50-51 22.38752574658667 27.00327011984066 28.599062129335557 22.01014200423711 52-53 22.740275125396263 27.204573314439635 28.05331905765838 22.00183250250572 54-55 22.281230306297058 27.052612382334146 28.328084130395936 22.33807318097286 56-57 22.38116493331441 27.299103088560905 28.233811730222097 22.085920247902585 58-59 22.706963583057146 26.93344824467291 28.28425702391886 22.07533114835108 60-61 22.47370925126977 26.92120459831648 28.845368997028437 21.759717153385314 62-63 22.300790726301926 27.10085161362435 28.688444424508177 21.90991323556555 64-65 22.090700049783475 27.21144887560676 28.48276587280285 22.21508520180691 66-67 22.14452797250364 27.29053621288208 28.230245262664823 22.33469055194946 68-69 22.203576909586005 27.00918972063161 28.483060014457063 22.304173355325325 70-71 22.299503856564765 27.138759119310475 28.346615054611075 22.21512196951369 72-73 22.309300759954805 27.02393256481162 28.32970618439006 22.337060490843516 74-75 22.13338735735049 27.40771489437741 28.031920252314713 22.426977495957388 76-77 22.24225653711443 27.082835437304077 28.131744576211737 22.543163449369764 78-79 22.279134547010823 27.137472249573314 28.141708624748052 22.441684578667818 80-81 22.307997196830033 27.41907611577121 28.006146089864686 22.266780597534066 82-83 22.260297393231625 27.239475749016645 28.081165806175974 22.41906105157576 84-85 22.198519289823867 27.244486310637345 28.043522701869566 22.51347169766922 86-87 22.13871867483302 27.218765649255193 28.1735862265229 22.468929449388884 88-89 21.993523000774328 27.232810913243654 27.907829241945482 22.865836844036536 90-91 22.103603043756763 27.010252491787195 28.20111827982294 22.685026184633102 92-93 22.092736805724076 26.761381602653195 28.40400185311831 22.741879738504416 94-95 21.889507158304834 26.86612657356593 28.291684100687633 22.95268216744161 96-97 21.844613788331255 26.802371370016232 28.35047566382256 23.002539177829956 98-99 21.816824082054058 26.826332245985686 28.096881792858802 23.259961879101454 100-101 21.600602771602073 26.59766466453731 28.596901271512714 23.204831292347905 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 166.0 1 130.0 2 145.0 3 214.0 4 193.5 5 173.0 6 331.0 7 819.5 8 1447.5 9 1804.0 10 1680.0 11 1514.5 12 1616.5 13 1760.5 14 1884.0 15 2028.0 16 2181.5 17 2277.5 18 2357.5 19 2468.5 20 2676.0 21 2997.5 22 3403.5 23 3976.5 24 4739.0 25 5681.5 26 6920.5 27 8668.0 28 10342.0 29 12429.5 30 15330.0 31 17789.0 32 20318.0 33 22805.5 34 25873.0 35 28835.0 36 31632.5 37 35098.0 38 37644.0 39 39067.0 40 40598.0 41 42850.5 42 45467.0 43 48253.5 44 50263.5 45 51929.0 46 52185.5 47 53693.5 48 58020.5 49 57636.0 50 53662.5 51 50703.0 52 48088.0 53 46355.0 54 44398.0 55 40640.0 56 36179.0 57 32192.0 58 28962.0 59 25486.5 60 21457.5 61 17246.0 62 13429.0 63 10277.0 64 7473.0 65 5672.0 66 4315.5 67 3411.5 68 2456.0 69 1436.5 70 979.5 71 700.0 72 561.5 73 463.5 74 357.0 75 292.5 76 197.5 77 110.5 78 58.5 79 37.5 80 24.5 81 12.5 82 7.0 83 4.0 84 3.0 85 2.0 86 2.0 87 3.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04382710647707276 2 0.0 3 0.0 4 0.0 5 8.088895490734906E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004963640414769146 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.5737394743247425E-4 22-23 6.618187219692195E-4 24-25 3.6767706776067755E-5 26-27 0.0022060624065640654 28-29 0.003713538384382843 30-31 2.9414165420854204E-4 32-33 1.8383853388033878E-4 34-35 0.0 36-37 3.6767706776067755E-5 38-39 7.353541355213551E-5 40-41 0.0 42-43 0.008272734024615244 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 4.044447745367453E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.4121248131281303E-4 84-85 7.353541355213551E-5 86-87 0.0 88-89 0.0 90-91 0.008971320453360531 92-93 0.0013971728574905746 94-95 0.0 96-97 0.0 98-99 3.6767706776067756E-4 100-101 0.012501020303863037 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1359889.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.620081920355304 #Duplication Level Percentage of deduplicated Percentage of total 1 80.57116966286144 48.84230905381507 2 11.515851500354907 13.96183722668322 3 3.327054881154424 6.050590183472975 4 1.4440378720326603 3.501507763948617 5 0.8172009474040213 2.4769394188511873 6 0.4859368434442935 1.767451875462717 7 0.3161541909507648 1.341570506842931 8 0.23624188841268848 1.145680210287729 9 0.18656560581450543 1.0178660077196433 >10 0.989076179845121 10.99094950781061 >50 0.07379605648094169 3.1055630926026883 >100 0.03484329088404381 3.785700669746752 >500 0.0013401108213469573 0.5433320250196361 >1k 4.8731302594434814E-4 0.6049249173488976 >5k 2.4365651297217407E-4 0.8637775403873406 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6546 0.481362817112279 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5150 0.3787073797934979 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3686 0.2710515343531715 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2075 0.15258598312068117 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1386 0.1019200831832598 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.6767706776067755E-5 0.0 18-19 0.0 0.0 0.0 7.353541355213551E-5 7.353541355213551E-5 20-21 0.0 0.0 0.0 1.4707082710427102E-4 7.353541355213551E-5 22-23 0.0 0.0 0.0 3.3090936098460977E-4 7.353541355213551E-5 24-25 7.353541355213551E-5 0.0 0.0 5.882833084170841E-4 7.353541355213551E-5 26-27 7.353541355213551E-5 0.0 0.0 8.456572558495583E-4 7.353541355213551E-5 28-29 7.353541355213551E-5 0.0 0.0 0.0015074759778187779 7.353541355213551E-5 30-31 7.353541355213551E-5 0.0 0.0 0.0033458613166221654 7.353541355213551E-5 32-33 7.353541355213551E-5 0.0 0.0 0.0056254591367383665 7.353541355213551E-5 34-35 7.353541355213551E-5 0.0 0.0 0.0077947538365263636 7.353541355213551E-5 36-37 7.353541355213551E-5 0.0 0.0 0.010699402671835716 7.353541355213551E-5 38-39 7.353541355213551E-5 0.0 0.0 0.01540566913917239 7.353541355213551E-5 40-41 7.353541355213551E-5 0.0 0.0 0.024119615645100446 7.353541355213551E-5 42-43 7.353541355213551E-5 0.0 0.0 0.032870329857804574 7.353541355213551E-5 44-45 7.353541355213551E-5 0.0 0.0 0.043643267943192426 7.353541355213551E-5 46-47 7.353541355213551E-5 0.0 0.0 0.06463762851232711 7.353541355213551E-5 48-49 7.353541355213551E-5 0.0 0.0 0.08033743930570804 7.353541355213551E-5 50-51 7.353541355213551E-5 0.0 0.0 0.10026553637833677 7.353541355213551E-5 52-53 7.353541355213551E-5 0.0 0.0 0.12460575826409362 7.353541355213551E-5 54-55 7.353541355213551E-5 0.0 0.0 0.15784376518965887 1.1030312032820326E-4 56-57 7.353541355213551E-5 0.0 0.0 0.24130645957133265 1.4707082710427102E-4 58-59 7.353541355213551E-5 0.0 0.0 0.3038850965042 1.4707082710427102E-4 60-61 7.353541355213551E-5 0.0 0.0 0.35462453185517345 1.4707082710427102E-4 62-63 7.353541355213551E-5 0.0 0.0 0.41738700732192113 1.4707082710427102E-4 64-65 7.353541355213551E-5 0.0 0.0 0.4788993807582825 1.4707082710427102E-4 66-67 7.353541355213551E-5 0.0 0.0 0.5804517868737815 1.4707082710427102E-4 68-69 7.353541355213551E-5 0.0 0.0 0.7206470528109279 1.4707082710427102E-4 70-71 7.353541355213551E-5 0.0 0.0 0.7971606506119249 1.4707082710427102E-4 72-73 7.353541355213551E-5 0.0 0.0 0.8857340562354721 2.2060624065640651E-4 74-75 7.353541355213551E-5 0.0 0.0 1.0500121701109428 2.2060624065640651E-4 76-77 7.353541355213551E-5 0.0 0.0 1.2339610071116098 2.2060624065640651E-4 78-79 7.353541355213551E-5 0.0 0.0 1.4152625692243999 2.2060624065640651E-4 80-81 7.353541355213551E-5 0.0 0.0 1.5924829158850464 2.2060624065640651E-4 82-83 7.353541355213551E-5 0.0 0.0 1.8364366503442562 2.2060624065640651E-4 84-85 7.353541355213551E-5 0.0 0.0 2.1944070435160516 2.2060624065640651E-4 86-87 7.353541355213551E-5 0.0 0.0 2.4701280766297837 2.2060624065640651E-4 88-89 7.353541355213551E-5 0.0 0.0 2.856336068605599 2.2060624065640651E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7945 0.0 30.31364 1 GGTATCA 5405 0.0 28.299856 1 ATCAACG 9645 0.0 24.918646 3 TCAACGC 9660 0.0 24.83078 4 CAACGCA 9825 0.0 24.413774 5 TATCAAC 9940 0.0 24.179108 2 AACGCAG 10015 0.0 24.045464 6 GTACATG 5415 0.0 23.68587 1 TACATGG 5385 0.0 23.197767 2 CCTACAC 595 0.0 22.352041 3 ACATGGG 5430 0.0 22.218258 3 GAGTACT 6000 0.0 22.206991 12-13 TACCTGG 1055 0.0 22.060722 2 AGTACTT 6095 0.0 22.055696 12-13 GTACTTT 6025 0.0 21.79868 14-15 AGAGTAC 8775 0.0 21.680208 10-11 ACGCAGA 11110 0.0 21.506117 7 CGCAGAG 11190 0.0 21.225014 8 GTACCTG 1235 0.0 20.001402 1 GCAGAGT 11885 0.0 19.983837 9 >>END_MODULE