FastQCFastQC Report
Thu 26 May 2016
SRR937202_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937202_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1341489
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49020.36541484872406704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36670.2733529682315695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25850.19269632475555148No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18360.1368628441977534No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18140.13522287547642955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70700.029.6655961
GGTATCA49050.028.0215851
GTACATG56550.026.071321
ATAGCGT950.001226851624.999336
TACATGG56700.024.8169082
ATCAACG83950.024.6890743
TATCAAC84800.024.5537192
TCAACGC84150.024.4636654
CAACGCA84450.024.4330585
AACGCAG85250.024.181176
ACATGGG57250.023.5820673
TACCTGG11750.023.0608882
GAGTACT45300.022.3879412-13
GTACTTT46000.021.94234814-15
ACGCAGA95650.021.4542457
CCCTATA2457.4524E-921.34352
TCGCGGA1451.07320375E-1021.28696882-83
AGTACTT47250.021.1623912-13
CGCAGAG97000.021.1066848
GTATAGA5000.020.9260181
Produced by FastQC (version 0.11.5)