##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937202_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1341489 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.672858294029993 33.0 31.0 34.0 30.0 34.0 2 31.783141717897053 33.0 31.0 34.0 30.0 34.0 3 31.821281426832424 33.0 31.0 34.0 30.0 34.0 4 35.43637107721346 37.0 35.0 37.0 33.0 37.0 5 35.440140768951515 37.0 35.0 37.0 33.0 37.0 6 35.47809560868557 37.0 35.0 37.0 33.0 37.0 7 35.42335867085008 37.0 35.0 37.0 33.0 37.0 8 35.410638477095226 37.0 35.0 37.0 33.0 37.0 9 37.02023870490179 39.0 37.0 39.0 33.0 39.0 10-11 36.894651391103466 39.0 37.0 39.0 33.0 39.0 12-13 36.75767151277424 39.0 37.0 39.0 33.0 39.0 14-15 37.925557719817306 40.0 38.0 41.0 33.0 41.0 16-17 37.89197563304656 40.0 38.0 41.0 33.0 41.0 18-19 37.84729431251393 40.0 38.0 41.0 33.0 41.0 20-21 37.78043875126818 40.0 38.0 41.0 32.5 41.0 22-23 37.62834656117195 40.0 37.0 41.0 32.0 41.0 24-25 37.465356778922526 40.0 37.0 41.0 32.0 41.0 26-27 37.30598200954313 40.0 37.0 41.0 32.0 41.0 28-29 37.35948747995697 40.0 37.0 41.0 32.0 41.0 30-31 37.21489889220113 39.5 37.0 41.0 31.5 41.0 32-33 37.037121437447496 39.0 36.0 41.0 31.0 41.0 34-35 36.921556196137274 39.0 36.0 41.0 31.0 41.0 36-37 36.94001478953611 39.5 36.0 41.0 31.0 41.0 38-39 36.858831865188606 40.0 36.0 41.0 30.5 41.0 40-41 36.69238696701949 39.5 36.0 41.0 30.0 41.0 42-43 36.46607836515991 39.0 36.0 41.0 30.0 41.0 44-45 36.25354512783929 39.0 35.5 41.0 30.0 41.0 46-47 35.921758955906455 39.0 35.0 41.0 28.5 41.0 48-49 35.62328912126749 39.0 35.0 40.5 27.0 41.0 50-51 34.74878176414417 38.0 34.0 39.5 26.0 40.5 52-53 34.84355704743013 38.0 34.5 39.5 26.0 40.5 54-55 35.163600297877956 39.0 35.0 40.0 26.0 41.0 56-57 34.96323786479054 38.5 34.5 40.0 26.0 41.0 58-59 34.72165444517249 38.0 34.0 40.0 26.0 41.0 60-61 34.43063565933079 38.0 34.0 40.0 25.0 41.0 62-63 34.09247634531479 37.5 34.0 40.0 23.5 41.0 64-65 33.72077668918642 37.0 33.0 40.0 23.0 41.0 66-67 33.34279110749324 36.5 33.0 39.0 22.0 41.0 68-69 32.86574619694981 36.0 32.0 39.0 20.5 40.5 70-71 32.42229641838286 35.0 32.0 39.0 19.5 40.0 72-73 32.02064049723852 35.0 31.5 38.0 18.5 40.0 74-75 31.56141421957243 35.0 31.0 37.0 17.0 39.0 76-77 31.12004906488238 35.0 31.0 37.0 12.5 39.0 78-79 30.654514871161822 34.5 30.5 36.0 7.5 39.0 80-81 30.162428465682538 34.0 30.0 36.0 7.0 37.5 82-83 29.678302990184786 34.0 29.5 35.0 2.0 37.0 84-85 29.24088196026952 34.0 29.0 35.0 2.0 36.5 86-87 28.80991644359365 34.0 29.0 35.0 2.0 36.0 88-89 28.404566865624687 34.0 29.0 35.0 2.0 36.0 90-91 27.979957718624604 34.0 27.5 35.0 2.0 35.0 92-93 27.520528308469174 33.0 26.5 35.0 2.0 35.0 94-95 26.978713205997217 33.0 25.5 35.0 2.0 35.0 96-97 26.32885137336199 33.0 25.0 35.0 2.0 35.0 98-99 25.553883781380243 32.0 22.0 34.5 2.0 35.0 100-101 23.642387302467633 30.5 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.026667058096169427 1101 2 0.0011250399874001005 1101 3 0.001782189725467731 1101 4 -0.042036005087403794 1101 5 0.045537593309546764 1101 6 0.030227906580236663 1101 7 0.1010001242290457 1101 8 0.14932543110028718 1101 9 0.08844291547876537 1101 10-11 0.08388602316552607 1101 12-13 -0.12805998122193074 1101 14-15 -0.027167926894421157 1101 16-17 0.21909648836896878 1101 18-19 0.1718393903291826 1101 20-21 0.16509661113160234 1101 22-23 0.20399953923960368 1101 24-25 0.13989099467688249 1101 26-27 0.10243553456483312 1101 28-29 0.23234556592582578 1101 30-31 0.2937729580122763 1101 32-33 0.18782842268188915 1101 34-35 0.0667426765051431 1101 36-37 0.1665650544779922 1101 38-39 0.15410540136639383 1101 40-41 0.22949340817876873 1101 42-43 0.32180140936347357 1101 44-45 0.35299217868486465 1101 46-47 0.40120098312824126 1101 48-49 0.5504265026329946 1101 50-51 0.3038386531962374 1101 52-53 0.39644433185933536 1101 54-55 0.3774032503439102 1101 56-57 0.29789174731541834 1101 58-59 0.40240950847948653 1101 60-61 0.3827633276940148 1101 62-63 0.36939955559940785 1101 64-65 0.41832801353645976 1101 66-67 0.3457413346773208 1101 68-69 0.5634837678898279 1101 70-71 0.45234524772551055 1101 72-73 0.4870976021961084 1101 74-75 0.36356951724722464 1101 76-77 0.5773605897936598 1101 78-79 0.6426911686938048 1101 80-81 0.5361576037750773 1101 82-83 0.6038682024773152 1101 84-85 0.5973577810327875 1101 86-87 0.5554590180815033 1101 88-89 0.48497618820294974 1101 90-91 0.7316032904369756 1101 92-93 0.8287282266588747 1101 94-95 0.7412264274468967 1101 96-97 0.868435859936671 1101 98-99 0.8155400622752573 1101 100-101 0.9318521288286448 1102 1 0.04052623053923554 1102 2 -0.06131476739622954 1102 3 0.04137316389682155 1102 4 -0.03171440987471641 1102 5 -0.15204722933333414 1102 6 -0.11691976298285311 1102 7 -0.045140225074945306 1102 8 -0.0363610561617449 1102 9 0.04051001436652513 1102 10-11 0.07652869946899443 1102 12-13 -0.0231362177299701 1102 14-15 -0.03313383343782306 1102 16-17 0.11233130159624949 1102 18-19 0.27424335863297244 1102 20-21 0.016995723673474572 1102 22-23 0.11101550989879172 1102 24-25 0.051832821184646605 1102 26-27 0.17504793147890751 1102 28-29 0.12136652237192891 1102 30-31 0.11285031841472204 1102 32-33 -0.009732636767942893 1102 34-35 0.02394217673082011 1102 36-37 0.286477564080414 1102 38-39 0.2942027258400941 1102 40-41 0.25109444957455196 1102 42-43 0.37792289756706055 1102 44-45 0.25110860936359103 1102 46-47 0.4207268551178629 1102 48-49 0.15723846852160506 1102 50-51 0.03901086909156959 1102 52-53 0.06630363582195997 1102 54-55 0.10472285183964658 1102 56-57 0.08042774332201219 1102 58-59 -0.03555017479749978 1102 60-61 0.03510123731019377 1102 62-63 -0.026918793471431002 1102 64-65 0.019419775330625555 1102 66-67 0.21662693956267276 1102 68-69 0.01274277473780927 1102 70-71 -0.01853572408447235 1102 72-73 0.06826848496136506 1102 74-75 0.29587206603649463 1102 76-77 0.27938942844606274 1102 78-79 0.1655021900328535 1102 80-81 0.25181859417713426 1102 82-83 0.4311323565225891 1102 84-85 0.45050573156948204 1102 86-87 0.32629141732705946 1102 88-89 0.31675370493433164 1102 90-91 0.3567565555594818 1102 92-93 0.5057997344409451 1102 94-95 0.393908081365133 1102 96-97 0.3996790784594033 1102 98-99 0.3768547796128239 1102 100-101 0.7299583966323588 1103 1 0.08585770481547428 1103 2 -0.03618829184816974 1103 3 0.048303176049778074 1103 4 -0.0163366789920687 1103 5 -0.0637822132079009 1103 6 0.005562580685207763 1103 7 -0.08447749634341051 1103 8 0.08451094210480647 1103 9 0.005442293815470123 1103 10-11 0.09827495701748745 1103 12-13 0.03284479602140067 1103 14-15 0.0805792248681172 1103 16-17 0.026043417812438463 1103 18-19 -0.024948171352171755 1103 20-21 -0.12769732778660625 1103 22-23 -0.27537820196620544 1103 24-25 -0.1482467853986691 1103 26-27 0.007398194164927929 1103 28-29 -0.1559057195818383 1103 30-31 -0.20067447895747392 1103 32-33 -0.16662747757047924 1103 34-35 -0.17001855696867096 1103 36-37 -0.0025535280214157297 1103 38-39 0.03611975285608793 1103 40-41 0.19207676655006622 1103 42-43 0.13579641259811126 1103 44-45 0.03723775102562854 1103 46-47 -0.011706154450628503 1103 48-49 0.07036664611180043 1103 50-51 0.17661061284316304 1103 52-53 0.0874799290756485 1103 54-55 -0.01710662363618809 1103 56-57 -0.13156976956107513 1103 58-59 -0.13599782444060793 1103 60-61 -0.15567287783201067 1103 62-63 -0.24187321202933987 1103 64-65 -0.11773702922657492 1103 66-67 -0.21956579315704516 1103 68-69 -0.009184521056390338 1103 70-71 0.1837551241417401 1103 72-73 0.12893969250060167 1103 74-75 0.1445653875623023 1103 76-77 0.14145822611973102 1103 78-79 0.20810521539705107 1103 80-81 0.05751070040717465 1103 82-83 0.15364908646628095 1103 84-85 0.3161810057961034 1103 86-87 0.23507279284088867 1103 88-89 0.39908920821437377 1103 90-91 0.33125528530847603 1103 92-93 0.11923220701828185 1103 94-95 0.11391492721726948 1103 96-97 0.16833967806812566 1103 98-99 0.269971871947039 1103 100-101 0.6230555480670041 1104 1 -0.05592227034427921 1104 2 -0.1048695658062222 1104 3 0.005934944753938964 1104 4 0.010583484604993032 1104 5 0.030786927237173245 1104 6 -0.10200124601528415 1104 7 0.006305277892828087 1104 8 -0.02407943608174179 1104 9 0.07777299661061932 1104 10-11 0.05192548709917588 1104 12-13 0.06421686060604515 1104 14-15 0.12264389569652678 1104 16-17 0.023452141393342174 1104 18-19 -0.0049931404359355724 1104 20-21 0.07415027336750768 1104 22-23 0.08822690895785712 1104 24-25 0.1859349850770542 1104 26-27 0.13464126183018976 1104 28-29 0.06588398483453517 1104 30-31 0.0784144182944928 1104 32-33 -0.004898695920473983 1104 34-35 0.0023827212411475784 1104 36-37 -0.05020629698125845 1104 38-39 -0.03565937003610031 1104 40-41 -0.04056737918576658 1104 42-43 0.1406069362830067 1104 44-45 0.22626159823799696 1104 46-47 0.2747534207753901 1104 48-49 0.14955666634597975 1104 50-51 0.15562376361887686 1104 52-53 0.12025057429965358 1104 54-55 0.09892529522028326 1104 56-57 0.21071716115532269 1104 58-59 0.14063098814001052 1104 60-61 0.12594696678716133 1104 62-63 -0.04306217315349414 1104 64-65 -0.0015423176904150182 1104 66-67 0.1228465836831063 1104 68-69 0.19560627598614388 1104 70-71 0.3434944834855074 1104 72-73 0.2951069686822123 1104 74-75 0.18973078013685196 1104 76-77 0.1203109782257421 1104 78-79 0.14629475012599258 1104 80-81 0.2855526013277192 1104 82-83 0.4304282428746191 1104 84-85 0.6464456971172403 1104 86-87 0.5641696860862382 1104 88-89 0.24741143812934752 1104 90-91 0.311011057984782 1104 92-93 0.5004873960349521 1104 94-95 0.4109114980373505 1104 96-97 0.33966508818597774 1104 98-99 0.4480335473901427 1104 100-101 0.8276960012818257 1105 1 -0.07792109541816927 1105 2 -0.01908231630301671 1105 3 -0.04890397993382223 1105 4 0.07794994458137694 1105 5 0.12487609484972495 1105 6 0.10023666802691622 1105 7 0.10273024827462507 1105 8 0.11787196066232042 1105 9 0.059442711681235494 1105 10-11 0.08171767286035703 1105 12-13 -0.11016458784651917 1105 14-15 0.05593759651119967 1105 16-17 0.10886234286245866 1105 18-19 0.1567828472894277 1105 20-21 0.041226040418465004 1105 22-23 0.1264454974912823 1105 24-25 0.1820261339888134 1105 26-27 0.22845613843353618 1105 28-29 0.23801572424140716 1105 30-31 0.13855635694871182 1105 32-33 0.1619104256360231 1105 34-35 0.14325802812361133 1105 36-37 0.02752326613312306 1105 38-39 0.21680238768733773 1105 40-41 0.05909696964835831 1105 42-43 0.2461027871209751 1105 44-45 0.29382932475596846 1105 46-47 0.40843410225867416 1105 48-49 0.4841116483790202 1105 50-51 0.2686937383756032 1105 52-53 0.2396207472516565 1105 54-55 0.3907255133461618 1105 56-57 0.3572890299739768 1105 58-59 0.366075979968457 1105 60-61 0.61977893144266 1105 62-63 0.6199844729813648 1105 64-65 0.5072269896847601 1105 66-67 0.46025507438661606 1105 68-69 0.5097900454078754 1105 70-71 0.42806024765470596 1105 72-73 0.44739140927557486 1105 74-75 0.3960353565328596 1105 76-77 0.42782338258329133 1105 78-79 0.4074794193659983 1105 80-81 0.5282578327548393 1105 82-83 0.6066516395873052 1105 84-85 0.7167672792290887 1105 86-87 0.574227785463556 1105 88-89 0.5863003488003287 1105 90-91 0.7186369034839579 1105 92-93 0.9023323828970824 1105 94-95 1.1258043726136648 1105 96-97 1.126285143351307 1105 98-99 1.212218100967231 1105 100-101 1.3127929653590122 1106 1 -0.10645822040079977 1106 2 -0.03252219773678533 1106 3 -0.09831239571367689 1106 4 -0.011704724142141743 1106 5 -0.12783635709853058 1106 6 -0.08535119318504059 1106 7 -0.06189654242106002 1106 8 -0.20469908822843763 1106 9 -0.25312680525399855 1106 10-11 -0.6541128073542595 1106 12-13 -0.664493431006342 1106 14-15 -0.5251846143500103 1106 16-17 -0.6196177791239563 1106 18-19 -0.35132956974450025 1106 20-21 -0.3166414418660679 1106 22-23 -0.18591791798574775 1106 24-25 -0.31663233612202646 1106 26-27 -0.2837505188038776 1106 28-29 -0.17631322579902076 1106 30-31 -0.33947771659227755 1106 32-33 -0.2053299524363439 1106 34-35 -0.2366446748872164 1106 36-37 -0.1442910679828131 1106 38-39 -0.24028540048952607 1106 40-41 -0.3215303839519237 1106 42-43 -0.366463701530094 1106 44-45 -0.27277796378962904 1106 46-47 -0.22017979863449 1106 48-49 -0.05603876827725429 1106 50-51 -0.11678759848716425 1106 52-53 -0.28918696382866216 1106 54-55 -0.24079144326386626 1106 56-57 -0.3956277420888554 1106 58-59 -0.16762811035278702 1106 60-61 -0.25199425363538097 1106 62-63 -0.25881771593368086 1106 64-65 -0.1454460945692304 1106 66-67 -0.07460314847372018 1106 68-69 -0.2160506597191869 1106 70-71 -0.07636300671759955 1106 72-73 0.0344358751753191 1106 74-75 -0.22619670266475111 1106 76-77 0.015025141125725838 1106 78-79 -0.0048672514808885126 1106 80-81 -0.11609049830431317 1106 82-83 -0.14029905638364681 1106 84-85 -0.11888043512889723 1106 86-87 -0.059474879782790424 1106 88-89 0.09165382081720352 1106 90-91 0.2022008020613768 1106 92-93 0.25985909414484 1106 94-95 0.0914105460381549 1106 96-97 0.24087025060600453 1106 98-99 0.4089785494383058 1106 100-101 0.5670521579828254 1107 1 0.08780942991896268 1107 2 0.04599626110439203 1107 3 0.04524916508751886 1107 4 0.07839199151535325 1107 5 0.13319156806097254 1107 6 0.1447515072572969 1107 7 0.2289018508943954 1107 8 0.027765684023641768 1107 9 0.20893157708935206 1107 10-11 0.2242892167306607 1107 12-13 0.16858842627188864 1107 14-15 0.2517041415436623 1107 16-17 0.22627286044237138 1107 18-19 0.19296224989863475 1107 20-21 0.04918716310360338 1107 22-23 0.1067957982970853 1107 24-25 0.08277091177467355 1107 26-27 0.2082064674733104 1107 28-29 0.16766324172009206 1107 30-31 0.20041547801681503 1107 32-33 0.27288523894249295 1107 34-35 0.24860244685328325 1107 36-37 0.17031832889665566 1107 38-39 0.2275523274406197 1107 40-41 0.2139944931873572 1107 42-43 0.09422508582206746 1107 44-45 0.11844435178500845 1107 46-47 0.17768275080711504 1107 48-49 0.036594383594326985 1107 50-51 -0.008610502565645106 1107 52-53 0.016899677441031713 1107 54-55 0.03362360156447863 1107 56-57 0.08674917659096337 1107 58-59 0.30172250212855545 1107 60-61 0.3385743392879945 1107 62-63 0.3631291806101018 1107 64-65 0.38928886769977566 1107 66-67 0.3948859580669577 1107 68-69 0.4541067477947891 1107 70-71 0.3736617380658558 1107 72-73 0.15745389474863458 1107 74-75 0.29507076734418547 1107 76-77 0.294523416663683 1107 78-79 0.18976025320911205 1107 80-81 0.2674444378771632 1107 82-83 0.24725144023840073 1107 84-85 0.43010982425014177 1107 86-87 0.4982573854966361 1107 88-89 0.3645218371295762 1107 90-91 0.46402535579208504 1107 92-93 0.5970276770935747 1107 94-95 0.6958131992927648 1107 96-97 0.8207790210012682 1107 98-99 0.9432642692045903 1107 100-101 1.2932281106569867 1108 1 0.06609703826878999 1108 2 0.004262037045474898 1108 3 0.007023721005165129 1108 4 -0.03977171638597099 1108 5 -0.12832644176513952 1108 6 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-3.219684103424825 2316 86-87 -3.520181321603779 2316 88-89 -3.1781303681620336 2316 90-91 -3.34172537839731 2316 92-93 -3.0211983760767147 2316 94-95 -3.1342520266832423 2316 96-97 -3.101848537314023 2316 98-99 -3.3231670483419684 2316 100-101 -4.039543516724304 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5515.0 3 1249.0 4 1101.0 5 1219.0 6 1303.0 7 1625.0 8 1986.0 9 2410.0 10 2896.0 11 3618.0 12 4372.0 13 5694.0 14 6444.0 15 7918.0 16 9854.0 17 12638.0 18 11010.0 19 8202.0 20 8249.0 21 8483.0 22 9281.0 23 10225.0 24 11532.0 25 13073.0 26 15320.0 27 18252.0 28 21590.0 29 26015.0 30 31810.0 31 39441.0 32 49988.0 33 66251.0 34 90520.0 35 128014.0 36 191154.0 37 270694.0 38 215758.0 39 26781.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.78473003763613 18.75861357278474 14.640923846108404 22.815732543470734 2 15.01183976846675 19.852391573433472 40.831429801534384 24.30433885656539 3 19.459278864084993 24.321762859728967 29.217582256597378 27.00137601958866 4 12.424000968721364 16.240210009223784 37.245990943651215 34.089798078403646 5 13.67964934364054 37.49373988103158 34.00967534088684 14.816935434441044 6 32.16738343302325 36.04158215995057 17.40446165580125 14.386572751224927 7 29.127531917150222 32.37582505077092 20.19229835598574 18.304344676093116 8 26.562539682539683 35.15040149393091 19.29919701213819 18.987861811391223 9 26.249247486268025 17.095468784311265 20.058318880588207 36.5969648488325 10-11 25.023252825638547 26.85314748006607 27.034239365520975 21.089360328774408 12-13 26.044024793126052 23.899744337228345 27.79407288163131 22.262157988014287 14-15 22.666743852087833 25.36986444621639 26.138145719850534 25.825245981845242 16-17 22.15984274994874 29.05510661175514 26.52739345640498 22.257657181891148 18-19 22.049854481857942 27.482930141208428 28.927057187341397 21.540158189592233 20-21 22.70697626826728 26.380952772175142 29.158673496633625 21.75339746292395 22-23 22.346704190511186 26.378650246997122 29.08669322613942 22.18795233635227 24-25 22.368922206937345 26.478438612292326 29.191143632553505 21.96149554821682 26-27 22.091506587209153 26.850966376685825 29.02012813326995 22.037398902835072 28-29 21.818646947883405 27.07146191623252 29.137900771198627 21.97199036468545 30-31 22.046421815153106 26.654279050050327 29.114497059652912 22.184802075143654 32-33 21.80856924214012 26.84932847267636 29.100077570826794 22.242024714356727 34-35 22.44275875515303 26.826003274183147 28.803361883144607 21.92787608751922 36-37 22.27563970905104 26.632778885213483 28.904295896197635 22.187285509537848 38-39 22.034851501712552 26.955579720644273 28.953991895069077 22.055576882574098 40-41 22.22585686359079 26.810247448127004 28.950515975929214 22.013379712352986 42-43 21.996205812277335 26.871326247862036 28.684357938292166 22.448110001568462 44-45 22.162581240763956 26.91272212717758 28.546593723169494 22.378102908888973 46-47 22.331900965709075 27.225653547294648 28.139562850264678 22.302882636731603 48-49 22.320391145226225 27.30558052729609 28.02682083204636 22.347207495431324 50-51 22.26719967311461 27.34240161410866 28.078976884586528 22.311421828190202 52-53 22.537753064605603 27.562903096264947 27.632721750808294 22.26662208832116 54-55 22.21426709362925 27.298714705185922 27.894527693184507 22.59249050800032 56-57 22.282506617695656 27.644996521719 27.728378180069278 22.34411868051606 58-59 22.588492906788428 27.29860495753707 27.797562568545057 22.315339567129442 60-61 22.40358703538232 27.270564943883493 28.27900790451699 22.046840116217197 62-63 22.282315229251733 27.384623542600394 28.162401237393976 22.1706599907539 64-65 22.074060023053907 27.456441595461804 28.069046713100587 22.4004516683837 66-67 22.14979711885247 27.549419024714272 27.713449001911382 22.587334854521878 68-69 22.02896894829246 27.40127803305083 28.04055593018836 22.529197088468354 70-71 22.119907046253935 27.453432570631144 27.86625743915641 22.56040294395851 72-73 22.25835674671279 27.339918953112 27.887463423596202 22.514260876579005 74-75 22.004387075380173 27.814610589178102 27.66269858163179 22.51830375380994 76-77 22.139116393636073 27.50481357390498 27.654224816680884 22.701845215778064 78-79 22.198986820299144 27.48180854742474 27.705378754892816 22.6138258773833 80-81 22.26997576432015 27.73558835940505 27.586141224181905 22.408294652092895 82-83 22.2290888953697 27.536500309373707 27.688928006106067 22.545482789150523 84-85 22.185832790665717 27.513733473530767 27.631464963586506 22.66896877221701 86-87 22.243094434251038 27.552290678597057 27.734614491363985 22.47000039578792 88-89 22.055150571717363 27.546124035204784 27.493363911552677 22.905361481525183 90-91 22.094059685073653 27.422766720076712 27.78579571572014 22.697377879129494 92-93 22.324519740595697 27.24730932260348 27.886074493603015 22.542096443197806 94-95 22.082671820369175 27.280595503553872 27.78757704790225 22.849155628174703 96-97 21.96351729017588 27.314800121721046 27.736268577861335 22.98541401024174 98-99 22.005323272288962 27.223481844265905 27.6389704158097 23.13222446763543 100-101 21.909504472279927 26.94643867921006 28.108380166943213 23.035676681566798 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1766.0 1 988.0 2 231.5 3 246.5 4 215.5 5 210.5 6 331.5 7 658.5 8 1091.0 9 1377.5 10 1353.5 11 1271.5 12 1376.5 13 1541.0 14 1685.5 15 1790.5 16 1839.5 17 1936.0 18 2041.5 19 2144.0 20 2378.0 21 2721.5 22 3176.5 23 3776.5 24 4532.5 25 5512.5 26 6898.0 27 8570.0 28 10310.5 29 12434.0 30 15027.0 31 17471.0 32 20131.5 33 22923.5 34 25920.0 35 28498.0 36 31345.0 37 34711.0 38 36975.5 39 38774.0 40 40314.5 41 42410.0 42 44931.0 43 47191.0 44 49255.5 45 50838.0 46 51273.5 47 52724.0 48 56548.5 49 56361.0 50 52736.5 51 49780.0 52 47161.0 53 45645.5 54 43860.5 55 40331.0 56 35869.5 57 32070.5 58 28870.0 59 25415.5 60 21424.0 61 17219.5 62 13446.5 63 10219.0 64 7834.0 65 6039.5 66 4379.0 67 3359.5 68 2498.5 69 1636.0 70 1162.0 71 900.5 72 664.0 73 489.5 74 383.0 75 303.5 76 209.5 77 123.0 78 76.0 79 47.5 80 27.5 81 18.0 82 16.5 83 9.5 84 10.0 85 11.5 86 9.0 87 8.5 88 8.5 89 8.5 90 9.5 91 7.5 92 4.0 93 4.5 94 4.0 95 4.5 96 6.0 97 6.5 98 7.0 99 5.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3138303780351535 2 0.27022211885449676 3 0.26627128511676207 4 0.2715639114446708 5 0.2724584398381202 6 0.21155596505077565 7 0.19619989429656154 8 0.20417610580481838 9 0.19701987865722342 10-11 0.21930854446067022 12-13 0.2095805481819083 14-15 0.20287158523103807 16-17 0.2034679374933376 18-19 0.1977280469687042 20-21 0.19273359677194518 22-23 0.1943362934768753 24-25 0.18449648114893227 26-27 0.1858010017227126 28-29 0.19858530334575983 30-31 0.2077169473622221 32-33 0.20220068893595103 34-35 0.2112950609360196 36-37 0.19083272393586528 38-39 0.19016182764077827 40-41 0.18796277867354858 42-43 0.19373994121457574 44-45 0.1971316947064046 46-47 0.199778007870359 48-49 0.20525699428023636 50-51 0.20823875559173427 52-53 0.22523479506727223 54-55 0.20350520950973136 56-57 0.18378831283745153 58-59 0.18617372188664982 60-61 0.18222288814891513 62-63 0.18978910747684102 64-65 0.18289378444400214 66-67 0.17924112683741722 68-69 0.17890567868987373 70-71 0.18960274739487243 72-73 0.1871800663292804 74-75 0.19053454780471551 76-77 0.19135453216537743 78-79 0.18904366714896656 80-81 0.19038545973914062 82-83 0.18587554575550005 84-85 0.1807692795095599 86-87 0.17801115029642434 88-89 0.17961384700135447 90-91 0.18468284123090087 92-93 0.1804711033784101 94-95 0.17793660626363692 96-97 0.176035733427557 98-99 0.18360195275548288 100-101 0.1752530210832888 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1341489.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.0242568988817 #Duplication Level Percentage of deduplicated Percentage of total 1 82.12279360347486 51.7572804131924 2 10.604579059424061 13.366914298912869 3 3.0636738417277742 5.792573017663053 4 1.3393465410860008 3.3764528192813095 5 0.7097356993895748 2.2365282524318024 6 0.4703525969006527 1.7786173740073734 7 0.29838981395254205 1.3164057403388167 8 0.21869733215411113 1.1026589475824613 9 0.15890210752127124 0.901321852157489 >10 0.9167265667987081 10.576646033110318 >50 0.06487771672296713 2.8144996289677793 >100 0.030033287363941997 3.30981875495567 >500 0.0011823959271996273 0.4825890893760064 >1k 7.094375563197765E-4 1.1876937780226668 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4902 0.36541484872406704 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3667 0.2733529682315695 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2585 0.19269632475555148 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1836 0.1368628441977534 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1814 0.13522287547642955 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 7.454403278744738E-5 5 0.0 0.0 0.0 0.0 7.454403278744738E-5 6 0.0 0.0 0.0 0.0 1.4908806557489476E-4 7 0.0 0.0 0.0 0.0 1.4908806557489476E-4 8 0.0 0.0 0.0 0.0 1.4908806557489476E-4 9 0.0 0.0 0.0 0.0 1.4908806557489476E-4 10-11 0.0 0.0 0.0 0.0 1.4908806557489476E-4 12-13 0.0 0.0 0.0 7.454403278744738E-5 1.4908806557489476E-4 14-15 7.454403278744738E-5 0.0 0.0 7.454403278744738E-5 1.4908806557489476E-4 16-17 7.454403278744738E-5 0.0 0.0 1.4908806557489476E-4 1.4908806557489476E-4 18-19 7.454403278744738E-5 0.0 0.0 1.4908806557489476E-4 1.4908806557489476E-4 20-21 7.454403278744738E-5 0.0 0.0 2.9817613114978953E-4 1.4908806557489476E-4 22-23 7.454403278744738E-5 0.0 0.0 2.9817613114978953E-4 1.8636008196861847E-4 24-25 7.454403278744738E-5 0.0 0.0 4.472641967246843E-4 2.2363209836234215E-4 26-27 1.1181604918117107E-4 0.0 0.0 5.590802459058554E-4 2.2363209836234215E-4 28-29 1.4908806557489476E-4 0.0 0.0 0.001341792590174053 2.2363209836234215E-4 30-31 1.4908806557489476E-4 0.0 0.0 0.003354481475435132 2.2363209836234215E-4 32-33 1.4908806557489476E-4 0.0 0.0 0.005292626327908764 2.2363209836234215E-4 34-35 1.4908806557489476E-4 0.0 0.0 0.0066716909344765405 2.2363209836234215E-4 36-37 1.4908806557489476E-4 0.0 0.0 0.00950436418039954 2.2363209836234215E-4 38-39 1.4908806557489476E-4 0.0 0.0 0.014386998327977345 2.2363209836234215E-4 40-41 1.4908806557489476E-4 0.0 0.0 0.02482316291821998 2.2363209836234215E-4 42-43 1.4908806557489476E-4 0.0 0.0 0.03253847031172078 2.2363209836234215E-4 44-45 1.4908806557489476E-4 0.0 0.0 0.04222919457408894 2.2363209836234215E-4 46-47 2.2363209836234215E-4 0.0 0.0 0.05944886614798929 2.2363209836234215E-4 48-49 2.2363209836234215E-4 0.0 0.0 0.07323951221366705 2.2363209836234215E-4 50-51 2.2363209836234215E-4 0.0 0.0 0.09262096073840337 2.2363209836234215E-4 52-53 2.2363209836234215E-4 0.0 0.0 0.11222604136150204 2.2363209836234215E-4 54-55 2.2363209836234215E-4 0.0 0.0 0.1402173256731885 2.2363209836234215E-4 56-57 2.2363209836234215E-4 0.0 0.0 0.21692313541147187 2.2363209836234215E-4 58-59 2.2363209836234215E-4 0.0 0.0 0.2717129995102457 2.2363209836234215E-4 60-61 2.2363209836234215E-4 0.0 0.0 0.3165512352318953 2.2363209836234215E-4 62-63 2.2363209836234215E-4 0.0 0.0 0.3746210367733168 2.2363209836234215E-4 64-65 2.2363209836234215E-4 0.0 0.0 0.42855364449503497 2.2363209836234215E-4 66-67 2.2363209836234215E-4 0.0 0.0 0.5262808714793785 2.9817613114978953E-4 68-69 2.2363209836234215E-4 0.0 0.0 0.6460731321688065 2.9817613114978953E-4 70-71 2.609041147560658E-4 0.0 0.0 0.7129391295791467 2.9817613114978953E-4 72-73 2.9817613114978953E-4 0.0 0.0 0.7925521565961405 2.9817613114978953E-4 74-75 3.3544814754351323E-4 0.0 0.0 0.9517036665973406 2.9817613114978953E-4 76-77 3.727201639372369E-4 0.0 0.0 1.1335165625659247 2.9817613114978953E-4 78-79 3.727201639372369E-4 0.0 0.0 1.3031787811901552 2.9817613114978953E-4 80-81 3.727201639372369E-4 0.0 0.0 1.4669892932405708 2.9817613114978953E-4 82-83 3.727201639372369E-4 0.0 0.0 1.702883884996448 2.9817613114978953E-4 84-85 3.727201639372369E-4 0.0 0.0 2.0557380641958303 2.9817613114978953E-4 86-87 3.727201639372369E-4 0.0 0.0 2.319027588001094 2.9817613114978953E-4 88-89 3.727201639372369E-4 0.0 0.0 2.7115764646597924 2.9817613114978953E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7070 0.0 29.665596 1 GGTATCA 4905 0.0 28.021585 1 GTACATG 5655 0.0 26.07132 1 ATAGCGT 95 0.0012268516 24.99933 6 TACATGG 5670 0.0 24.816908 2 ATCAACG 8395 0.0 24.689074 3 TATCAAC 8480 0.0 24.553719 2 TCAACGC 8415 0.0 24.463665 4 CAACGCA 8445 0.0 24.433058 5 AACGCAG 8525 0.0 24.18117 6 ACATGGG 5725 0.0 23.582067 3 TACCTGG 1175 0.0 23.060888 2 GAGTACT 4530 0.0 22.38794 12-13 GTACTTT 4600 0.0 21.942348 14-15 ACGCAGA 9565 0.0 21.454245 7 CCCTATA 245 7.4524E-9 21.3435 2 TCGCGGA 145 1.07320375E-10 21.286968 82-83 AGTACTT 4725 0.0 21.16239 12-13 CGCAGAG 9700 0.0 21.106684 8 GTATAGA 500 0.0 20.926018 1 >>END_MODULE