FastQCFastQC Report
Thu 26 May 2016
SRR937202_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937202_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1341489
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66470.49549418593816275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53140.3961269902324954No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37340.2783474184283285No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21820.1626550795422102No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13780.10272167718110249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA81850.027.7014941
GGTATCA57800.027.2209741
GTACATG54250.025.497451
TACATGG54650.024.8601682
ACATGGG51750.024.5091973
GGACCGA2052.6720954E-823.1691236
ATCAACG96850.023.1019953
TCAACGC97200.022.9699384
CAACGCA98300.022.7128985
TATCAAC99150.022.7098272
GAGTACT55900.022.60466212-13
AACGCAG100450.022.2234466
GTACTTT57750.021.54828614-15
TACCTGG9650.021.167492
AGTACTT57100.020.84010112-13
CATGGGG27000.020.7609124
AGAGTAC84050.020.51620910-11
ACGCAGA110750.020.0738337
CGCAGAG111900.019.8250838
CATGGGA26750.018.823934
Produced by FastQC (version 0.11.5)