##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937202_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1341489 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27082741640073 33.0 31.0 34.0 31.0 34.0 2 32.49047960885255 34.0 31.0 34.0 31.0 34.0 3 32.573873509212525 34.0 31.0 34.0 31.0 34.0 4 36.16383436614091 37.0 35.0 37.0 35.0 37.0 5 36.02781610583464 37.0 35.0 37.0 35.0 37.0 6 36.12045048449894 37.0 35.0 37.0 35.0 37.0 7 36.08760340189148 37.0 35.0 37.0 35.0 37.0 8 36.064584950007045 37.0 35.0 37.0 35.0 37.0 9 37.78794086272791 39.0 38.0 39.0 35.0 39.0 10-11 37.70605536087139 39.0 37.0 39.0 35.0 39.0 12-13 37.62317693249814 39.0 37.0 39.0 35.0 39.0 14-15 38.87715031580579 40.0 38.0 41.0 35.0 41.0 16-17 38.85935031893665 40.0 38.0 41.0 35.0 41.0 18-19 38.857570952874006 40.0 38.0 41.0 35.0 41.0 20-21 38.770701809705486 40.0 38.0 41.0 35.0 41.0 22-23 38.64840859671604 40.0 38.0 41.0 34.0 41.0 24-25 38.50412713037528 40.0 38.0 41.0 34.0 41.0 26-27 38.2960944890342 40.0 38.0 41.0 34.0 41.0 28-29 38.28967922957251 40.0 38.0 41.0 34.0 41.0 30-31 38.42707282728371 40.0 38.0 41.0 34.0 41.0 32-33 38.398797530207105 40.0 38.0 41.0 34.0 41.0 34-35 38.28664342383725 40.0 38.0 41.0 34.0 41.0 36-37 38.14394527275289 40.0 38.0 41.0 34.0 41.0 38-39 38.01017600591581 40.0 38.0 41.0 33.5 41.0 40-41 37.8546234818176 40.0 37.0 41.0 33.0 41.0 42-43 37.65655178685774 40.0 37.0 41.0 33.0 41.0 44-45 37.38486301415814 40.0 37.0 41.0 32.5 41.0 46-47 37.046367133834124 40.0 37.0 41.0 31.5 41.0 48-49 36.7350943615639 40.0 36.0 41.0 31.0 41.0 50-51 36.52200651663935 39.5 36.0 41.0 31.0 41.0 52-53 36.29755890655831 39.0 36.0 41.0 30.0 41.0 54-55 36.092176305582825 39.0 35.0 40.5 30.0 41.0 56-57 35.85175092751413 39.0 35.0 40.0 29.0 41.0 58-59 35.600687743246496 38.5 35.0 40.0 28.5 41.0 60-61 35.26464436160118 38.0 35.0 40.0 28.0 41.0 62-63 34.91087366351867 38.0 34.0 40.0 27.5 41.0 64-65 34.56520180187836 37.0 34.0 40.0 27.0 41.0 66-67 34.18562656868599 36.5 34.0 39.0 26.0 41.0 68-69 33.749857807257456 36.0 33.0 39.0 26.0 40.0 70-71 33.35611808967498 35.5 33.0 39.0 26.0 40.0 72-73 32.926070955483056 35.0 33.0 38.0 25.0 40.0 74-75 32.488703597271396 35.0 32.0 37.0 24.0 39.0 76-77 31.179976876441028 34.0 30.5 36.0 22.5 38.5 78-79 31.799177257510124 35.0 32.0 36.0 24.0 39.0 80-81 31.62253212661453 35.0 32.0 36.0 23.0 37.5 82-83 31.293977065782876 35.0 31.5 35.5 21.5 37.0 84-85 30.970240158510432 34.0 31.0 35.0 20.5 37.0 86-87 30.65190098465213 34.0 31.0 35.0 19.5 36.0 88-89 30.304580581726725 34.0 31.0 35.0 18.0 36.0 90-91 29.889379264384576 34.0 30.5 35.0 13.5 35.5 92-93 29.501301911532636 34.0 30.0 35.0 7.0 35.0 94-95 29.117696827927773 34.0 29.5 35.0 2.0 35.0 96-97 28.71654929708704 34.0 29.0 35.0 2.0 35.0 98-99 28.09860610113091 34.0 29.0 35.0 2.0 35.0 100-101 26.502553878563297 32.0 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.031902775502615555 1101 2 0.02039446816878865 1101 3 0.06401958426336307 1101 4 0.05416383181312767 1101 5 -0.10597317786489668 1101 6 0.028652128484260686 1101 7 -0.004386143938262421 1101 8 -0.01021768527322564 1101 9 0.0036463005121305514 1101 10-11 0.03173374423796815 1101 12-13 0.04063755189930163 1101 14-15 0.07223383478436318 1101 16-17 0.04448145510715307 1101 18-19 0.07761604401365929 1101 20-21 -0.04717373949287662 1101 22-23 -0.02929978867393146 1101 24-25 0.07352630238291624 1101 26-27 0.07387792658849435 1101 28-29 0.054220966398446535 1101 30-31 0.12381272399522913 1101 32-33 0.11832467979244399 1101 34-35 0.10492026093456275 1101 36-37 0.06297487334881424 1101 38-39 0.1730997541780681 1101 40-41 0.12054188650736819 1101 42-43 0.12413780094710347 1101 44-45 0.22168708025969863 1101 46-47 0.3319053209197804 1101 48-49 0.2192449162679324 1101 50-51 0.1700133728251032 1101 52-53 0.07175754930013767 1101 54-55 0.12790398388708724 1101 56-57 0.11131629639985618 1101 58-59 0.09731130399784149 1101 60-61 0.23290181892450335 1101 62-63 0.19091884091970002 1101 64-65 0.16909611173034733 1101 66-67 0.11256864662966137 1101 68-69 0.11413145101848698 1101 70-71 -0.02735465469321241 1101 72-73 0.0021955175024928053 1101 74-75 -0.08369918285885092 1101 76-77 -0.021082865734438627 1101 78-79 0.1449149361619888 1101 80-81 0.051047048452911525 1101 82-83 -0.018582209122669724 1101 84-85 0.0576717994426339 1101 86-87 -0.02147202906060741 1101 88-89 0.0440162864820941 1101 90-91 0.1307351534316581 1101 92-93 0.18650711026858247 1101 94-95 -0.16967673072489475 1101 96-97 -0.20750552536025424 1101 98-99 -0.22659333425974637 1101 100-101 0.23261816739805496 1102 1 0.10627947741054555 1102 2 0.03754730884180901 1102 3 0.0023957959620233282 1102 4 0.033504500350957755 1102 5 -0.020161448113675817 1102 6 0.04015022593427631 1102 7 0.10953430466299352 1102 8 0.11662216874006504 1102 9 0.08851168725436054 1102 10-11 0.0921511394931045 1102 12-13 0.05498797978622605 1102 14-15 0.13505415216497596 1102 16-17 -0.06640642147966958 1102 18-19 -0.002946112428851677 1102 20-21 0.04549613744666203 1102 22-23 0.09713205579820539 1102 24-25 0.0516294914333173 1102 26-27 0.07115711571062633 1102 28-29 -0.0255006604984942 1102 30-31 -0.16290873252410876 1102 32-33 -0.018547127495530447 1102 34-35 0.004327825102656391 1102 36-37 0.14855838677427613 1102 38-39 0.002152125864299137 1102 40-41 -0.10224223310368785 1102 42-43 0.012944888655709974 1102 44-45 -0.027591803976186213 1102 46-47 -0.04213182139933025 1102 48-49 0.007161108418010542 1102 50-51 -0.021482841503960515 1102 52-53 -0.00327510640855877 1102 54-55 -0.004684769397165667 1102 56-57 -0.0480842135373436 1102 58-59 0.01107512476059469 1102 60-61 -0.0010240996947814551 1102 62-63 0.11898601154540955 1102 64-65 0.07570422230242713 1102 66-67 0.012827949396651661 1102 68-69 0.04850558231684943 1102 70-71 -0.10062121195951335 1102 72-73 -0.07560936127533324 1102 74-75 -0.17380696911565252 1102 76-77 -0.16058491474938563 1102 78-79 -0.014753096514642294 1102 80-81 0.028938610576474844 1102 82-83 -0.1070198464335732 1102 84-85 -0.13083279328015252 1102 86-87 -0.0804684152618158 1102 88-89 0.14326946462234957 1102 90-91 -0.03876351792188615 1102 92-93 -0.2096075329397742 1102 94-95 -0.12858912375315867 1102 96-97 0.034102181685906174 1102 98-99 0.024529364521605146 1102 100-101 -0.01171333960685672 1103 1 -0.004029182592404368 1103 2 0.008001804337048668 1103 3 -0.03858102548998943 1103 4 -0.06373925908956579 1103 5 -0.04100800995716014 1103 6 -0.03419541423141226 1103 7 0.015142564905865186 1103 8 0.002973486566602901 1103 9 0.013421482161419362 1103 10-11 0.05764961487786735 1103 12-13 -0.005278914238097343 1103 14-15 0.061573978443625776 1103 16-17 -0.04394496080092836 1103 18-19 -0.03340183264075591 1103 20-21 -0.1184723841947033 1103 22-23 0.027496236290041054 1103 24-25 -0.06444821212872398 1103 26-27 -0.0019812364487989953 1103 28-29 -0.10343519154191938 1103 30-31 -0.15884377229599522 1103 32-33 -0.07403589935594113 1103 34-35 -0.05903147850460044 1103 36-37 0.037057198171048356 1103 38-39 -0.10713279661741382 1103 40-41 -0.15771514645341966 1103 42-43 -0.03709043970966519 1103 44-45 -0.0728058310644002 1103 46-47 -0.17153412826308312 1103 48-49 -0.31386429346176925 1103 50-51 -0.30211959996156423 1103 52-53 -0.28996793176710156 1103 54-55 -0.39357407699261415 1103 56-57 -0.2751290978330232 1103 58-59 -0.16099160031563287 1103 60-61 -0.3472133466824445 1103 62-63 -0.2772924199379787 1103 64-65 -0.17710132095362496 1103 66-67 -0.19014889717774963 1103 68-69 -0.14310542254747105 1103 70-71 -0.281769682162782 1103 72-73 -0.2465352156722247 1103 74-75 -0.28827197205183097 1103 76-77 -0.6175940624407978 1103 78-79 -0.2583803304449468 1103 80-81 -0.2568571883313311 1103 82-83 -0.15757583639717865 1103 84-85 -0.3564321978794318 1103 86-87 -0.21949479691493323 1103 88-89 -0.09067261570022467 1103 90-91 -0.21264674903848046 1103 92-93 -0.12656216503802753 1103 94-95 -0.25113898385283306 1103 96-97 0.005005425928953144 1103 98-99 -0.021550914085736395 1103 100-101 0.22047362752363142 1104 1 -0.04936215586663195 1104 2 0.059885746562883924 1104 3 -3.9369325023841384E-4 1104 4 -0.029367978782595117 1104 5 -0.09354408050575813 1104 6 -0.09523596588792316 1104 7 -0.09689614527815849 1104 8 0.024512873297851456 1104 9 -0.025730157304501233 1104 10-11 0.05537099616417862 1104 12-13 0.06362308744153466 1104 14-15 0.03279223267407616 1104 16-17 0.030584400774060327 1104 18-19 -0.015967694249745534 1104 20-21 7.488347777240278E-4 1104 22-23 -0.10422985449273625 1104 24-25 -0.03223632883766925 1104 26-27 -0.10701028536607993 1104 28-29 -0.17000638508996957 1104 30-31 -0.24740538405843182 1104 32-33 -0.1522893828798857 1104 34-35 -0.0641905700448433 1104 36-37 -0.03971056274971119 1104 38-39 -0.09444167082943267 1104 40-41 -0.06754619638001458 1104 42-43 0.05435356008731418 1104 44-45 0.0748480870202286 1104 46-47 -0.19395642588066409 1104 48-49 -0.2793249536531235 1104 50-51 -0.15350137465075164 1104 52-53 0.049831622563999645 1104 54-55 0.05518884228232679 1104 56-57 -0.13137741604099773 1104 58-59 -0.09310609528007063 1104 60-61 0.028569135484566743 1104 62-63 -0.05066968814275441 1104 64-65 -0.03673141768120303 1104 66-67 -0.21079388177469127 1104 68-69 -0.07618613994608125 1104 70-71 -0.18671024847063933 1104 72-73 -0.25219992212350206 1104 74-75 -0.14566510583964032 1104 76-77 -0.10400853451849557 1104 78-79 -0.08186231264692267 1104 80-81 0.0913003336959548 1104 82-83 0.029615635564653076 1104 84-85 0.003935732728752583 1104 86-87 0.01462166496812145 1104 88-89 0.1417824032459727 1104 90-91 0.21838325939800995 1104 92-93 -0.04991554102304363 1104 94-95 -0.14810127244977878 1104 96-97 0.06414034562907034 1104 98-99 0.11351146902672582 1104 100-101 0.2539548149142874 1105 1 -0.008347236657506585 1105 2 -0.03281546606802266 1105 3 -0.023584107735153736 1105 4 0.004431077486430013 1105 5 -0.03190293482864348 1105 6 -0.052357697512057655 1105 7 -0.0775663890001752 1105 8 -0.037233479889643206 1105 9 -0.10970176042518176 1105 10-11 8.14857501985955E-4 1105 12-13 0.013813702539529515 1105 14-15 -0.04535195258524283 1105 16-17 -0.03304180151580738 1105 18-19 0.014058921289993975 1105 20-21 -0.007093045522267971 1105 22-23 0.07203160642790607 1105 24-25 -0.14753444836777874 1105 26-27 -0.03305790058627878 1105 28-29 -0.06865724579849086 1105 30-31 -0.02112918755171478 1105 32-33 -0.01774449684706525 1105 34-35 0.008159687321473541 1105 36-37 0.05619768192506314 1105 38-39 0.03323646476279407 1105 40-41 0.04012682913312915 1105 42-43 -0.13404580892900952 1105 44-45 -0.021061791311524303 1105 46-47 0.16825898290279184 1105 48-49 0.14580438297105758 1105 50-51 0.24056626136262338 1105 52-53 0.2275607609275596 1105 54-55 0.21498402090311686 1105 56-57 0.30849240763513563 1105 58-59 0.24085603263004174 1105 60-61 0.1667353489747896 1105 62-63 0.1582461255915959 1105 64-65 0.2515314429567468 1105 66-67 0.20163001431426864 1105 68-69 0.20178778558138077 1105 70-71 0.11547447369984809 1105 72-73 0.14658914596666506 1105 74-75 0.20798356903581805 1105 76-77 0.27499923450070085 1105 78-79 0.2163919941630077 1105 80-81 0.16619680885228405 1105 82-83 0.06437185146614866 1105 84-85 0.04922005112457484 1105 86-87 0.15561524861691467 1105 88-89 0.2289471523260609 1105 90-91 0.16834026154593928 1105 92-93 0.39191254729114533 1105 94-95 0.5354268581434525 1105 96-97 0.28903561187921767 1105 98-99 0.26206496377843536 1105 100-101 0.3777704952768772 1106 1 -0.18051160479710404 1106 2 0.021152476871975523 1106 3 -0.048947234634511005 1106 4 0.0030843817007379926 1106 5 -0.061875822778567624 1106 6 -0.10618562684258848 1106 7 -0.08796124162962116 1106 8 0.011320959753085447 1106 9 -0.20202915713397118 1106 10-11 -0.17090470913273492 1106 12-13 -0.33919106789871734 1106 14-15 -0.14452451846823777 1106 16-17 -0.020230556249721587 1106 18-19 -0.11640668630850826 1106 20-21 -0.3198289399527141 1106 22-23 -0.27893726637346816 1106 24-25 -0.16111416527664346 1106 26-27 -0.13823199129942054 1106 28-29 -0.03701025110333234 1106 30-31 -0.049622847749731136 1106 32-33 -0.15308368248800974 1106 34-35 -0.2775373702551107 1106 36-37 -0.12884704761354016 1106 38-39 -0.12332653958011974 1106 40-41 -0.24849074132792026 1106 42-43 -0.44825683402100935 1106 44-45 -0.18195470762044152 1106 46-47 -0.31348972651374396 1106 48-49 -0.4893338671082219 1106 50-51 -0.24561674675673828 1106 52-53 -0.14206733014898276 1106 54-55 -0.21295742276901564 1106 56-57 -0.11547788667846248 1106 58-59 -0.283192997072085 1106 60-61 -0.1989338840142736 1106 62-63 -0.23341380165832248 1106 64-65 -0.37595272980508554 1106 66-67 -0.17210122368392433 1106 68-69 -0.0618685291036698 1106 70-71 -0.18570691339680678 1106 72-73 -0.24560607504464116 1106 74-75 -0.3180128255246686 1106 76-77 -0.3910871891669423 1106 78-79 -0.31062974179198477 1106 80-81 -0.3227572574208253 1106 82-83 -0.5495384828207932 1106 84-85 -0.4198412164082974 1106 86-87 -0.14688486717042437 1106 88-89 -0.34485427061123275 1106 90-91 -0.2998778960459667 1106 92-93 -0.1733508956185723 1106 94-95 -0.22531500022547846 1106 96-97 -0.359033055355102 1106 98-99 -0.45579389680292337 1106 100-101 -0.31232648107043914 1107 1 -0.005136692554771116 1107 2 -0.007051972585365718 1107 3 0.016420426810810795 1107 4 -0.04948581483806436 1107 5 0.0341281824235935 1107 6 0.022074450422515213 1107 7 -0.0495527340277917 1107 8 -0.08715483831413451 1107 9 -0.03191433366593799 1107 10-11 0.018389431532732203 1107 12-13 -0.07043759116072579 1107 14-15 0.03279909342614218 1107 16-17 -0.020964651492207054 1107 18-19 0.0033168243048962154 1107 20-21 0.01780097016042248 1107 22-23 -0.054735333594877034 1107 24-25 0.051917128242521926 1107 26-27 0.06012238469084963 1107 28-29 -0.19484916831764565 1107 30-31 -0.17672222203280086 1107 32-33 -0.08170227138316477 1107 34-35 -0.09385012897237033 1107 36-37 -0.10842416118250497 1107 38-39 -0.15936338437086306 1107 40-41 -0.0455390147558532 1107 42-43 -0.005769849009830352 1107 44-45 -0.1536587600103303 1107 46-47 -0.26444115384656186 1107 48-49 -0.1785062020566741 1107 50-51 -0.24115117653334295 1107 52-53 -0.2203907080229257 1107 54-55 -0.15886398339757335 1107 56-57 -0.10019424240486074 1107 58-59 -0.1848401556420285 1107 60-61 -0.2897048470658987 1107 62-63 -0.19679420762623323 1107 64-65 -0.22749093459982106 1107 66-67 -0.10119495078541263 1107 68-69 -0.3235611106559517 1107 70-71 -0.19399795041423573 1107 72-73 -0.12501055058950072 1107 74-75 -0.10418605435445016 1107 76-77 -0.16821092459342069 1107 78-79 -0.11426089489889435 1107 80-81 -0.15810700287565282 1107 82-83 -0.08627988256989738 1107 84-85 -0.08588945530863157 1107 86-87 -0.039419423548249455 1107 88-89 -0.005074633457994082 1107 90-91 -0.1412494476014352 1107 92-93 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80-81 -0.09207761714366569 2316 82-83 -0.30142234027740855 2316 84-85 -0.38501659830194157 2316 86-87 -0.2896395668910685 2316 88-89 -0.39204577879345237 2316 90-91 -0.41349527282124043 2316 92-93 -0.22527898129278867 2316 94-95 -0.31670448449421684 2316 96-97 -0.2925708969424825 2316 98-99 -0.2186705454380835 2316 100-101 -0.4204871542465938 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 8.0 5 24.0 6 59.0 7 111.0 8 244.0 9 507.0 10 907.0 11 1536.0 12 2206.0 13 3403.0 14 4548.0 15 6241.0 16 8562.0 17 12396.0 18 11367.0 19 6604.0 20 5663.0 21 5615.0 22 5709.0 23 6108.0 24 6929.0 25 8471.0 26 10225.0 27 12785.0 28 16283.0 29 20545.0 30 26041.0 31 33431.0 32 43194.0 33 57864.0 34 81639.0 35 120398.0 36 193690.0 37 317111.0 38 282003.0 39 29052.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.368991454878206 19.226374878624668 15.113410426868285 22.29122323962884 2 15.770610120545156 20.243624807955936 40.051167024105304 23.934598047393603 3 19.845112408674243 24.226959743985972 29.7478399002899 26.180087947049884 4 13.034267197520363 16.803219114311208 36.90859471616199 33.25391897200644 5 13.72560567847097 37.52071611596403 33.98307820422307 14.770600001341935 6 32.45028472093323 36.21140389522389 17.003866598980686 14.334444784862194 7 29.130615308809837 32.57760592893419 20.10303476211881 18.18874400013716 8 27.00081774803968 34.88153835029583 19.016406396176187 19.101237505488307 9 26.368087997739824 16.8784835358322 20.029087081593662 36.72434138483431 10-11 25.21314002574751 26.866452128940306 26.80264243687425 21.11776540843794 12-13 26.261643575206595 23.850067652945977 27.799024157506498 22.089264614340934 14-15 22.77476744125371 25.357047281043677 26.26808717775547 25.60009809994715 16-17 22.174352529167216 28.872879315447236 26.82347749403834 22.129290661347202 18-19 21.959143906509855 27.040102453318664 29.532892181747293 21.467861458424185 20-21 22.572343120219397 25.986497093900883 29.945344315160245 21.495815470719474 22-23 22.333981750637268 25.87867129491756 29.859411847363138 21.927935107082035 24-25 22.36889234940273 26.077286824623634 29.978266598135573 21.57555422783806 26-27 22.134225296150987 26.39409569119043 29.89934109154124 21.57233792111735 28-29 21.760400402400236 26.65622026306599 29.909638857341363 21.673740477192414 30-31 22.07117770123674 26.215836540938003 29.857354617888465 21.855631139936794 32-33 21.863839360591104 26.353440095297092 29.858649605028443 21.92407093908336 34-35 22.42202507810351 26.28437504891952 29.621375948665996 21.67222392431097 36-37 22.2687627028117 26.129224750640056 29.709474839254575 21.892537707293673 38-39 22.05285340712226 26.450855917774646 29.82379961296594 21.672491062137155 40-41 22.136827859500848 26.37055777966043 29.737787539736644 21.754826821102082 42-43 22.018507991239925 26.37931130888588 29.447649224174082 22.154531475700107 44-45 22.204961130863214 26.422887710181637 29.234838763060583 22.13731239589456 46-47 22.312668982004325 26.8201602845793 28.896360685775285 21.97081004764109 48-49 22.47047869941535 26.955345888039332 28.645035479232405 21.929139933312907 50-51 22.381622212332715 26.9151666543669 28.644811847134044 22.058399286166342 52-53 22.663610357904936 27.254558838602673 28.021766881912804 22.06006392157959 54-55 22.298207439643562 27.013937497810268 28.30984823580365 22.37800682674252 56-57 22.425752279742884 27.327506971730664 28.161356522491054 22.085384226035398 58-59 22.709727772646662 26.946847868301564 28.2638172955574 22.07960706349437 60-61 22.40528994274273 26.915763006629202 28.876121980873492 21.80282506975458 62-63 22.27267610841386 27.102868528925693 28.689575538822904 21.93487982383754 64-65 22.062536479986044 27.175847137024604 28.524050514018377 22.23756586897097 66-67 22.202641989610054 27.226201631172525 28.253306586934368 22.317849792283052 68-69 22.151206606986715 27.04468691133509 28.498966446985403 22.305140034692794 70-71 22.320794281578156 27.073759084122194 28.306195578197062 22.299251056102584 72-73 22.32672053218476 27.009278495761052 28.346262995820315 22.31773797623387 74-75 22.179123347265612 27.356690960566954 28.037464339998312 22.426721352169118 76-77 22.221799806036426 27.151023974106387 28.131538909376076 22.495637310481115 78-79 22.32616145193885 27.12176544123731 28.123339065769454 22.42873404105438 80-81 22.28419316147952 27.45993444597757 28.013684793539117 22.242187599003792 82-83 22.26794286906998 27.207002713420998 28.137989384858514 22.387065032650504 84-85 22.226148212338913 27.197010780942797 28.127768615063502 22.449072391654788 86-87 22.19261581720014 27.257472852926863 28.14033510524499 22.409576224628005 88-89 22.013896498592235 27.2572492208285 27.817447627226166 22.911406653353104 90-91 22.12324329804833 27.027732239520546 28.154645685023365 22.694378777407756 92-93 22.097121134355884 26.823243680245646 28.37555821188812 22.70407697351035 94-95 21.908155788083242 26.814308578005488 28.329266956344778 22.948268677566492 96-97 21.850011442509032 26.80998502410381 28.305226505770825 23.034777027616325 98-99 21.852017922176962 26.862989124215925 28.050830937634295 23.234162015972817 100-101 21.43383546625821 26.939081637635997 28.364179387114774 23.262903508991016 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 192.0 1 143.0 2 130.5 3 207.0 4 190.5 5 159.5 6 340.5 7 841.0 8 1453.5 9 1789.5 10 1694.5 11 1527.5 12 1590.0 13 1754.5 14 1927.0 15 2083.5 16 2192.5 17 2259.0 18 2377.0 19 2488.5 20 2661.0 21 3039.5 22 3443.0 23 3947.5 24 4757.0 25 5749.0 26 6926.0 27 8619.5 28 10311.0 29 12271.0 30 14987.0 31 17429.0 32 19824.0 33 22323.5 34 25359.5 35 28052.5 36 30932.5 37 34231.0 38 36887.0 39 38547.5 40 39913.0 41 42363.5 42 44841.0 43 47432.0 44 49604.5 45 51020.5 46 51170.5 47 52727.0 48 57189.0 49 56903.0 50 53017.0 51 50225.5 52 47553.0 53 45830.5 54 43972.0 55 40325.0 56 35515.0 57 31506.5 58 28651.0 59 25462.5 60 21515.5 61 17261.5 62 13409.5 63 10185.0 64 7439.5 65 5607.0 66 4219.5 67 3408.5 68 2485.0 69 1412.0 70 965.0 71 721.0 72 579.0 73 443.5 74 341.0 75 276.5 76 181.5 77 105.5 78 62.0 79 42.5 80 28.0 81 15.5 82 8.5 83 3.5 84 1.0 85 1.5 86 2.0 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04524822790198056 2 0.0 3 0.0 4 1.4908806557489476E-4 5 0.010585252655817529 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00626169875414558 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0013045205737803292 24-25 4.099921803309606E-4 26-27 0.002087232918048527 28-29 0.0028699452623167244 30-31 0.0011554325082054344 32-33 0.0 34-35 0.0 36-37 3.3544814754351323E-4 38-39 3.727201639372369E-4 40-41 3.727201639372369E-5 42-43 0.013157021786984463 44-45 3.727201639372369E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.1181604918117107E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.708962950870265E-4 84-85 1.1181604918117107E-4 86-87 0.0 88-89 0.0 90-91 0.00939254813121837 92-93 0.003019033327891619 94-95 0.0 96-97 0.0 98-99 0.0016772407377175663 100-101 0.01323156581977191 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1341489.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.81414367564027 #Duplication Level Percentage of deduplicated Percentage of total 1 80.79668643873464 49.13581297600864 2 11.302551960677153 13.747100376760201 3 3.378207427400004 6.163283755680568 4 1.4105681615208958 3.43129979356062 5 0.7956022865996744 2.419193588297026 6 0.4980543405053012 1.817324893305939 7 0.31940895632600563 1.3597207512907208 8 0.23356548550528214 1.1363267994551123 9 0.17474872853100787 0.9564474855618152 >10 0.9815441605556601 10.919217008692828 >50 0.07179169497630895 3.0145317045267337 >100 0.03506358572061347 3.7882056647750497 >500 0.0014711819649964467 0.601080357303967 >1k 4.90393988332149E-4 0.6186758626777868 >5k 2.451969941660745E-4 0.8917789821027968 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6647 0.49549418593816275 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5314 0.3961269902324954 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3734 0.2783474184283285 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2182 0.1626550795422102 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1378 0.10272167718110249 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 7.454403278744738E-5 9 0.0 0.0 0.0 0.0 7.454403278744738E-5 10-11 0.0 0.0 0.0 0.0 7.454403278744738E-5 12-13 0.0 0.0 0.0 7.454403278744738E-5 7.454403278744738E-5 14-15 0.0 0.0 0.0 7.454403278744738E-5 7.454403278744738E-5 16-17 0.0 0.0 0.0 1.4908806557489476E-4 1.4908806557489476E-4 18-19 0.0 0.0 0.0 1.4908806557489476E-4 1.4908806557489476E-4 20-21 0.0 0.0 0.0 2.9817613114978953E-4 1.4908806557489476E-4 22-23 0.0 0.0 0.0 2.9817613114978953E-4 1.4908806557489476E-4 24-25 0.0 0.0 0.0 5.218082295121316E-4 1.4908806557489476E-4 26-27 0.0 0.0 0.0 6.336242786933028E-4 1.4908806557489476E-4 28-29 0.0 0.0 0.0 0.0015281526721426715 1.4908806557489476E-4 30-31 0.0 0.0 0.0 0.003578113573797474 1.4908806557489476E-4 32-33 0.0 0.0 0.0 0.005553530442664829 1.4908806557489476E-4 34-35 0.0 0.0 0.0 0.007007139082020054 1.4908806557489476E-4 36-37 0.0 0.0 0.0 0.009877084344336779 1.4908806557489476E-4 38-39 0.0 0.0 0.0 0.014908806557489475 1.4908806557489476E-4 40-41 0.0 0.0 0.0 0.025531331229700728 1.4908806557489476E-4 42-43 0.0 0.0 0.0 0.03313482257402036 1.4908806557489476E-4 44-45 0.0 0.0 0.0 0.042564642721632455 1.4908806557489476E-4 46-47 0.0 0.0 0.0 0.05974704227913908 1.4908806557489476E-4 48-49 0.0 0.0 0.0 0.07357496036121056 1.4908806557489476E-4 50-51 0.0 0.0 0.0 0.0932918570334904 1.4908806557489476E-4 52-53 0.0 0.0 0.0 0.11293420967298279 1.4908806557489476E-4 54-55 0.0 0.0 0.0 0.14155911826336257 1.4908806557489476E-4 56-57 0.0 0.0 0.0 0.21938308849345764 1.4908806557489476E-4 58-59 0.0 0.0 0.0 0.2745829447725624 1.4908806557489476E-4 60-61 0.0 0.0 0.0 0.3194584525106058 1.4908806557489476E-4 62-63 0.0 0.0 0.0 0.3777146141339959 1.4908806557489476E-4 64-65 0.0 0.0 0.0 0.4325044782327697 1.4908806557489476E-4 66-67 0.0 0.0 0.0 0.5310889615941689 1.4908806557489476E-4 68-69 0.0 0.0 0.0 0.6519248387426211 1.4908806557489476E-4 70-71 0.0 0.0 0.0 0.7200580847103479 2.2363209836234215E-4 72-73 0.0 0.0 0.0 0.7998947438257041 2.9817613114978953E-4 74-75 0.0 0.0 0.0 0.9605744064990469 2.9817613114978953E-4 76-77 0.0 0.0 0.0 1.1454063357955229 2.9817613114978953E-4 78-79 0.0 0.0 0.0 1.3172676033869828 2.9817613114978953E-4 80-81 0.0 0.0 0.0 1.4828299002079033 2.9817613114978953E-4 82-83 0.0 0.0 0.0 1.7230853178818462 2.9817613114978953E-4 84-85 0.0 0.0 0.0 2.08216392381898 2.9817613114978953E-4 86-87 0.0 0.0 0.0 2.348025216755411 2.9817613114978953E-4 88-89 0.0 0.0 0.0 2.7463512559551364 2.9817613114978953E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8185 0.0 27.701494 1 GGTATCA 5780 0.0 27.220974 1 GTACATG 5425 0.0 25.49745 1 TACATGG 5465 0.0 24.860168 2 ACATGGG 5175 0.0 24.509197 3 GGACCGA 205 2.6720954E-8 23.169123 6 ATCAACG 9685 0.0 23.101995 3 TCAACGC 9720 0.0 22.969938 4 CAACGCA 9830 0.0 22.712898 5 TATCAAC 9915 0.0 22.709827 2 GAGTACT 5590 0.0 22.604662 12-13 AACGCAG 10045 0.0 22.223446 6 GTACTTT 5775 0.0 21.548286 14-15 TACCTGG 965 0.0 21.16749 2 AGTACTT 5710 0.0 20.840101 12-13 CATGGGG 2700 0.0 20.760912 4 AGAGTAC 8405 0.0 20.516209 10-11 ACGCAGA 11075 0.0 20.073833 7 CGCAGAG 11190 0.0 19.825083 8 CATGGGA 2675 0.0 18.82393 4 >>END_MODULE