FastQCFastQC Report
Thu 26 May 2016
SRR937201_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937201_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479979
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53200.3594645599701077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39680.26811191239875704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26900.18175933577435896No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20990.14182633672504813No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20750.14020469209360403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76250.030.8630451
GGTATCA51700.027.9548031
GTACATG65000.025.8918631
TACATGG64650.025.6547872
TCAACGC91450.025.5175344
ATCAACG91700.025.4972043
CAACGCA92600.025.1484555
ACATGGG64500.025.1241533
TATCAAC94450.024.705352
AACGCAG94900.024.6282676
CATGGGG32300.022.659964
GTTCGCG1050.002195300522.62127
GAGTACT51550.022.43833212-13
ACGCAGA105550.021.9632457
GTACCTG12700.021.7118931
CGCAGAG106900.021.6866158
GTACTTT54050.021.09180314-15
AGAGTAC84900.020.75769410-11
GCTTACG1150.00370495320.6702421
TACCTGG12250.020.5613332
Produced by FastQC (version 0.11.5)