##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937201_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1479979 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.60275652559935 33.0 31.0 34.0 30.0 34.0 2 31.692715910158185 33.0 31.0 34.0 30.0 34.0 3 31.744638944201235 33.0 31.0 34.0 30.0 34.0 4 35.3722721741322 37.0 35.0 37.0 33.0 37.0 5 35.35388339969689 37.0 35.0 37.0 33.0 37.0 6 35.41603428156751 37.0 35.0 37.0 33.0 37.0 7 35.34985428847301 37.0 35.0 37.0 33.0 37.0 8 35.335633816425776 37.0 35.0 37.0 33.0 37.0 9 36.94180930945642 39.0 37.0 39.0 33.0 39.0 10-11 36.834055077808536 39.0 37.0 39.0 33.0 39.0 12-13 36.71647705812042 39.0 37.0 39.0 32.0 39.0 14-15 37.86313319310612 40.0 38.0 41.0 33.0 41.0 16-17 37.80469249901519 40.0 38.0 41.0 33.0 41.0 18-19 37.74764506793677 40.0 38.0 41.0 32.0 41.0 20-21 37.668842598442275 40.0 37.5 41.0 32.0 41.0 22-23 37.53394440056244 40.0 37.0 41.0 32.0 41.0 24-25 37.378037796482246 40.0 37.0 41.0 32.0 41.0 26-27 37.21305369873491 39.5 37.0 41.0 31.5 41.0 28-29 37.021979703766064 39.0 36.0 41.0 31.0 41.0 30-31 37.0270149103467 39.0 36.5 41.0 31.0 41.0 32-33 36.896939416032254 39.0 36.0 41.0 31.0 41.0 34-35 36.69611325566106 39.0 36.0 41.0 30.0 41.0 36-37 36.7271900479669 39.0 36.0 41.0 30.0 41.0 38-39 36.71554697735576 39.0 36.0 41.0 30.0 41.0 40-41 36.56478402734093 39.0 35.5 41.0 30.0 41.0 42-43 36.336204770473095 39.0 35.0 41.0 30.0 41.0 44-45 36.138169190238514 39.0 35.0 41.0 29.5 41.0 46-47 35.8358054404826 39.0 35.0 41.0 28.5 41.0 48-49 35.550157130607936 39.0 35.0 40.0 27.0 41.0 50-51 34.62373756654655 38.0 34.0 39.5 25.0 40.5 52-53 34.757312096995975 38.0 34.5 39.5 26.0 40.5 54-55 35.10799038364733 39.0 35.0 40.0 26.0 41.0 56-57 34.886458524073646 38.0 34.0 40.0 25.5 41.0 58-59 34.64202093408082 38.0 34.0 40.0 25.0 41.0 60-61 34.35611789086197 38.0 34.0 40.0 24.5 41.0 62-63 34.03343729877248 37.5 34.0 40.0 23.0 41.0 64-65 33.66955240581116 37.0 33.0 40.0 22.5 41.0 66-67 33.273430231104626 36.5 33.0 39.0 22.0 41.0 68-69 32.781443520482384 36.0 32.0 39.0 20.5 40.5 70-71 32.34672113590801 35.0 32.0 38.5 19.5 40.0 72-73 31.93834169268618 35.0 31.0 38.0 18.0 40.0 74-75 31.478982134205957 35.0 31.0 37.0 16.0 39.0 76-77 31.043045205371158 35.0 31.0 36.5 12.0 39.0 78-79 30.564911394013023 34.5 30.0 36.0 7.5 38.5 80-81 30.109350538081962 34.0 30.0 36.0 7.0 37.5 82-83 29.661582360290247 34.0 29.5 35.0 2.0 37.0 84-85 29.20568737799658 34.0 29.0 35.0 2.0 36.5 86-87 28.78067188791192 34.0 29.0 35.0 2.0 36.0 88-89 28.391544069206386 34.0 28.5 35.0 2.0 36.0 90-91 27.95370170792964 34.0 27.0 35.0 2.0 35.0 92-93 27.495771899466142 33.0 26.5 35.0 2.0 35.0 94-95 26.955875049578406 33.0 25.5 35.0 2.0 35.0 96-97 26.31539062378588 33.0 25.0 35.0 2.0 35.0 98-99 25.589251942088367 32.0 23.0 34.5 2.0 35.0 100-101 23.762431764234492 30.5 10.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.023028059017963898 1101 2 -0.007157948145522397 1101 3 0.1135551842892788 1101 4 -0.06472859017340937 1101 5 0.06857314256428992 1101 6 -0.09351690160507076 1101 7 -0.11635937719506728 1101 8 -0.03184567971479879 1101 9 -0.0854124259265987 1101 10-11 -0.06988748528354449 1101 12-13 0.16565785787488352 1101 14-15 0.14057617090143282 1101 16-17 0.0660406177237931 1101 18-19 0.2018513382987308 1101 20-21 0.21832644263215428 1101 22-23 0.02948069869687231 1101 24-25 0.1874707189151934 1101 26-27 0.1623911808170888 1101 28-29 0.15200566211080258 1101 30-31 -0.06305351731403874 1101 32-33 -0.05299866511299456 1101 34-35 -0.20301843997331304 1101 36-37 -0.18873923956654437 1101 38-39 -0.0748712227063848 1101 40-41 -0.024694853439662268 1101 42-43 0.052091030532814386 1101 44-45 -0.2570199156338546 1101 46-47 -0.29307494941656387 1101 48-49 -0.10887510039734849 1101 50-51 -0.031057864010151093 1101 52-53 -0.24558533576105646 1101 54-55 -0.17775890928790972 1101 56-57 -0.031617136168335946 1101 58-59 -0.1320094357238304 1101 60-61 -0.13297024859812723 1101 62-63 -0.15482383755063012 1101 64-65 -0.35981868544701 1101 66-67 -0.17382961233472827 1101 68-69 -0.18974947423159705 1101 70-71 -0.14794631072951603 1101 72-73 -0.16700051586048303 1101 74-75 -0.15135648185562545 1101 76-77 -0.11137067890206609 1101 78-79 -0.17617732546755605 1101 80-81 -0.02693096775347925 1101 82-83 -0.05633584243230416 1101 84-85 -0.14229331402395218 1101 86-87 -0.1257618095592754 1101 88-89 -0.016570440524194652 1101 90-91 0.008944830251127911 1101 92-93 -0.133931015906672 1101 94-95 -0.16370272542030406 1101 96-97 -0.33158654660178755 1101 98-99 -0.13282293446542326 1101 100-101 0.2603077643722713 1102 1 -0.05855632625382512 1102 2 -0.18520749899724365 1102 3 -0.12580230347825605 1102 4 -0.27606597065928185 1102 5 -0.1196178444912448 1102 6 -0.11041644014402863 1102 7 -0.10090792696540518 1102 8 0.018874349407568047 1102 9 0.0919037123618196 1102 10-11 -0.10953967642049633 1102 12-13 -0.028482871683245037 1102 14-15 0.03328032190946573 1102 16-17 0.025925359530447167 1102 18-19 0.03183372569046128 1102 20-21 0.11294442569406726 1102 22-23 0.061441693996599156 1102 24-25 0.007799630359642151 1102 26-27 -0.022596613484772377 1102 28-29 -0.020078353778018254 1102 30-31 0.01957102898730767 1102 32-33 -0.01585158645048068 1102 34-35 -0.039181032720556175 1102 36-37 -9.508264143747169E-4 1102 38-39 0.12427437842425348 1102 40-41 -0.008464486947517003 1102 42-43 0.035110923679418704 1102 44-45 0.010638196337367845 1102 46-47 0.0912396578062129 1102 48-49 0.32766142568965506 1102 50-51 0.4567672197701924 1102 52-53 0.2513321901225183 1102 54-55 0.23054551581300586 1102 56-57 0.23526553923504423 1102 58-59 0.30749205786818123 1102 60-61 0.3284319077418729 1102 62-63 0.4214732855449057 1102 64-65 0.40508431137308776 1102 66-67 0.3668903097201124 1102 68-69 0.15527295641541627 1102 70-71 0.12720090712078047 1102 72-73 -0.010258741323287524 1102 74-75 -0.19257667693266 1102 76-77 -0.21908114689423286 1102 78-79 -0.12958822922455582 1102 80-81 -0.062503083086618 1102 82-83 -0.09034370118882151 1102 84-85 -0.044794679134927406 1102 86-87 -0.10956383099419398 1102 88-89 0.19194062490477037 1102 90-91 -0.06996447284633334 1102 92-93 -0.28518852401703754 1102 94-95 -0.24677936435648462 1102 96-97 -0.08431730389041192 1102 98-99 -0.012011683809632245 1102 100-101 0.09751854821364248 1103 1 -0.08004198989511124 1103 2 -0.17056641401855188 1103 3 -0.12654360346129678 1103 4 -0.10438472418113776 1103 5 -0.22038102786861913 1103 6 -0.14971245134832856 1103 7 -0.06693505143267942 1103 8 -0.1119560706988949 1103 9 -0.06596418852828378 1103 10-11 -0.06612685165724486 1103 12-13 0.05081789915931978 1103 14-15 0.09204051820351822 1103 16-17 0.18200484001866357 1103 18-19 0.02737252221319153 1103 20-21 0.05358216251343606 1103 22-23 0.05731804187692546 1103 24-25 0.015383867750699665 1103 26-27 0.005722687121945569 1103 28-29 -0.1070645727104349 1103 30-31 -0.14037688853417762 1103 32-33 -0.08376806847601159 1103 34-35 0.015338708836559078 1103 36-37 0.04797634017844388 1103 38-39 -0.0317966222486703 1103 40-41 0.0839780828194776 1103 42-43 -0.05442553132011341 1103 44-45 -0.14687574064939923 1103 46-47 -0.15984931460429408 1103 48-49 -0.2474880652478788 1103 50-51 -0.28181511923641267 1103 52-53 -0.36301839279639836 1103 54-55 -0.3716939760545799 1103 56-57 -0.36335662754924414 1103 58-59 -0.27041966451907484 1103 60-61 -0.24997219101788204 1103 62-63 -0.10497881444032231 1103 64-65 -0.09938619254678116 1103 66-67 -0.014721074021622371 1103 68-69 -0.047233239320604525 1103 70-71 -0.017155912565520737 1103 72-73 0.03385161663434033 1103 74-75 0.07099048046912415 1103 76-77 0.07576832270699896 1103 78-79 0.26372064941561035 1103 80-81 0.02050446121357652 1103 82-83 0.06706711030214763 1103 84-85 0.09910122155823231 1103 86-87 0.010299726042138246 1103 88-89 0.10923890104038136 1103 90-91 0.06716453523410948 1103 92-93 0.16559107190608913 1103 94-95 0.0627021633232765 1103 96-97 0.17029817751690857 1103 98-99 0.11282104499493073 1103 100-101 0.42928065383384606 1104 1 0.05540340679787903 1104 2 0.08825552596736586 1104 3 0.06025818682577011 1104 4 -0.02519468108329903 1104 5 0.034297621881805185 1104 6 -0.03158355247353484 1104 7 0.02957653475627353 1104 8 0.10696897224197954 1104 9 0.049967213698806745 1104 10-11 0.05888323888858338 1104 12-13 0.0746612983726962 1104 14-15 0.12106429152949971 1104 16-17 0.03506406928035233 1104 18-19 -0.04947230985769835 1104 20-21 0.013359004486517279 1104 22-23 0.11187930494418197 1104 24-25 0.00783361683247108 1104 26-27 0.09498057232292467 1104 28-29 0.06651159521418748 1104 30-31 -0.01765342779088286 1104 32-33 0.06497442527906827 1104 34-35 0.11852603197171163 1104 36-37 -0.10378659029545645 1104 38-39 -0.1477058813335148 1104 40-41 -0.25618626974867453 1104 42-43 0.05726055404141306 1104 44-45 0.021657116410175092 1104 46-47 0.08491957826101526 1104 48-49 0.20768580606851117 1104 50-51 0.12076178193454012 1104 52-53 0.1780868471119348 1104 54-55 0.3207512322815589 1104 56-57 0.25780980591369485 1104 58-59 0.06696894706666257 1104 60-61 0.27746764690552794 1104 62-63 0.30947606063872257 1104 64-65 0.23349110417357366 1104 66-67 0.2873024167690801 1104 68-69 0.225210657244574 1104 70-71 0.22896483314823968 1104 72-73 0.23871492337347533 1104 74-75 0.15419011407019312 1104 76-77 0.2521196710281579 1104 78-79 0.2631844490218569 1104 80-81 0.45328068429984825 1104 82-83 0.4359334599014417 1104 84-85 0.40086375962200904 1104 86-87 0.3682705284871908 1104 88-89 0.18810352403516717 1104 90-91 0.3981972241179186 1104 92-93 0.34551979901736374 1104 94-95 0.31411231710319143 1104 96-97 0.35299956238826624 1104 98-99 0.3910499075437599 1104 100-101 0.6648920960643494 1105 1 0.1820167410999467 1105 2 0.2087016028469293 1105 3 0.14554369574938164 1105 4 0.2340513397479782 1105 5 0.2044975793266275 1105 6 0.12695005833340645 1105 7 0.23794251967513702 1105 8 0.14898507574448416 1105 9 0.14219777973458747 1105 10-11 0.20219104776393237 1105 12-13 0.24235919292927122 1105 14-15 0.317204744378877 1105 16-17 0.21297974775001904 1105 18-19 0.2240825655646077 1105 20-21 0.31949810802534273 1105 22-23 0.3193805634357574 1105 24-25 0.34241949525958404 1105 26-27 0.4102927171558477 1105 28-29 0.3795370478819322 1105 30-31 0.2432591213162354 1105 32-33 0.2556099505500953 1105 34-35 0.3151416626965471 1105 36-37 0.39754844870480355 1105 38-39 0.4172449337019515 1105 40-41 0.5209520631262379 1105 42-43 0.29137307097266785 1105 44-45 0.38417339895580227 1105 46-47 0.29704060363324203 1105 48-49 0.35245709792957314 1105 50-51 0.2883163122447243 1105 52-53 0.2613013589469446 1105 54-55 0.3934407974402845 1105 56-57 0.29350069183986705 1105 58-59 0.574647043085676 1105 60-61 0.6852568664331642 1105 62-63 0.790443321693985 1105 64-65 0.5916736996243159 1105 66-67 0.5690475423846948 1105 68-69 0.49033882877307633 1105 70-71 0.470691224105785 1105 72-73 0.3395946998030972 1105 74-75 0.24791757150203964 1105 76-77 0.27373588558929285 1105 78-79 0.36329907554826235 1105 80-81 0.588589935899563 1105 82-83 0.6601431401667384 1105 84-85 0.51949053772789 1105 86-87 0.4767080657878431 1105 88-89 0.6608828053716032 1105 90-91 0.6732308271403724 1105 92-93 0.6240029014975015 1105 94-95 0.7908571443135948 1105 96-97 0.6929632218222821 1105 98-99 0.6717791580017476 1105 100-101 0.988975083853088 1106 1 -0.02342073758420682 1106 2 0.060295253050011155 1106 3 -0.134360499209226 1106 4 0.05994893066975493 1106 5 0.09276301199955839 1106 6 -0.03727575011851769 1106 7 0.1807540410840005 1106 8 0.07677473872156781 1106 9 0.19121570054815606 1106 10-11 -0.08906712976994413 1106 12-13 -0.17280761325930172 1106 14-15 -0.16567087959408155 1106 16-17 -0.22049194480570833 1106 18-19 -0.156377779209528 1106 20-21 0.027011750167162063 1106 22-23 0.03218441113985193 1106 24-25 0.024036709162167824 1106 26-27 0.04453488067552058 1106 28-29 0.19259281013660967 1106 30-31 0.12936428982768433 1106 32-33 0.33418868741178187 1106 34-35 0.13377171261438292 1106 36-37 -0.1027631502162265 1106 38-39 -0.08935328971912782 1106 40-41 0.24197181322700345 1106 42-43 0.12877618076090158 1106 44-45 0.0245142199647006 1106 46-47 0.2361920004025322 1106 48-49 0.33529667874375946 1106 50-51 0.11755390254639764 1106 52-53 0.4259244172671046 1106 54-55 0.36279481102041444 1106 56-57 0.237827963721557 1106 58-59 0.24436110069272132 1106 60-61 0.5402757794025064 1106 62-63 0.677284468253994 1106 64-65 0.558576390846838 1106 66-67 0.4501380610961405 1106 68-69 0.38586598903724223 1106 70-71 0.44188576414857295 1106 72-73 0.5295603619120506 1106 74-75 0.6249491654622936 1106 76-77 0.5167105701147641 1106 78-79 0.6475563539409706 1106 80-81 0.568786351736847 1106 82-83 0.530088571599471 1106 84-85 0.5943296999763632 1106 86-87 0.3451482108906241 1106 88-89 0.36916575579482114 1106 90-91 0.33504794492786516 1106 92-93 0.44650197135146996 1106 94-95 0.5042341945104774 1106 96-97 0.5713059341281159 1106 98-99 0.5704490491746625 1106 100-101 0.7389553182498894 1107 1 0.057507026408075035 1107 2 -0.026521216024523397 1107 3 -0.042342302352320615 1107 4 -0.01159550458435632 1107 5 0.10366963822876585 1107 6 0.18500850684315395 1107 7 -0.18180454728103967 1107 8 0.12291020421187682 1107 9 0.002531296541171457 1107 10-11 0.08165706837361597 1107 12-13 0.18022749380806857 1107 14-15 0.2638335317651652 1107 16-17 0.1657512773246168 1107 18-19 0.11623388332149176 1107 20-21 0.19480997643312037 1107 22-23 0.2204426135109543 1107 24-25 0.21541158279510597 1107 26-27 0.24055431822721118 1107 28-29 0.16929187272275925 1107 30-31 0.15832762040391657 1107 32-33 0.11887759468132941 1107 34-35 0.08256548156944632 1107 36-37 0.10140926040779874 1107 38-39 0.16010103433196576 1107 40-41 0.08910442971446031 1107 42-43 0.16384074284160022 1107 44-45 0.3006136134028097 1107 46-47 0.09749610848083279 1107 48-49 0.14595976118103238 1107 50-51 0.097238674166654 1107 52-53 0.1134313100159332 1107 54-55 0.26817804785526533 1107 56-57 0.19763519651265682 1107 58-59 0.3381128238799249 1107 60-61 0.3008699343514962 1107 62-63 0.3821243267228809 1107 64-65 0.3718322956350022 1107 66-67 0.3372290913323255 1107 68-69 0.20796988573790998 1107 70-71 0.444487776831231 1107 72-73 0.3298211375760509 1107 74-75 0.25540132978542474 1107 76-77 0.08756687927752438 1107 78-79 0.27256324668979914 1107 80-81 0.2611468884522239 1107 82-83 0.42502469879366345 1107 84-85 0.6194296448343728 1107 86-87 0.6131290950005415 1107 88-89 0.6809937127840691 1107 90-91 0.8846768852826123 1107 92-93 0.9873702279455294 1107 94-95 0.8404303161904707 1107 96-97 0.8402897131925364 1107 98-99 0.7771993478604458 1107 100-101 0.8961639847327518 1108 1 -7.233272002267199E-4 1108 2 -0.1122841575891016 1108 3 -0.07877305183073346 1108 4 -0.06149832025510449 1108 5 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-1.1911454027595028 2316 84-85 -1.2994929162095339 2316 86-87 -1.5395197578593773 2316 88-89 -1.3905998692051789 2316 90-91 -1.5377184813541867 2316 92-93 -1.5002378123023767 2316 94-95 -1.5509816508420862 2316 96-97 -1.3753211692372709 2316 98-99 -1.6247657945753389 2316 100-101 -1.839860787519342 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6237.0 3 1277.0 4 1228.0 5 1329.0 6 1486.0 7 1847.0 8 2236.0 9 2689.0 10 3220.0 11 4143.0 12 5226.0 13 6551.0 14 7335.0 15 8933.0 16 10526.0 17 13716.0 18 12236.0 19 9128.0 20 9042.0 21 9555.0 22 10520.0 23 11461.0 24 13182.0 25 15173.0 26 17431.0 27 20655.0 28 24510.0 29 29604.0 30 36377.0 31 45179.0 32 57155.0 33 74716.0 34 100323.0 35 142175.0 36 209669.0 37 291420.0 38 232725.0 39 29753.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.71128193763635 18.854396027029534 14.634605857664592 22.799716177669527 2 15.015490888051659 19.765126565941454 40.93544232532017 24.28394022068672 3 19.502526460421496 24.295484079030988 29.1942698332902 27.007719627257316 4 12.437524771593207 16.194880029268592 37.232830956954174 34.13476424218403 5 13.714951529972835 37.42732883069023 33.950358021095134 14.907361618241803 6 32.254150611631665 35.92725825027371 17.475528978280256 14.343062159814373 7 29.102083958254475 32.37681746253689 20.18481189393934 18.336286685269286 8 26.55411261273461 35.134875280990165 19.243967174931615 19.067044931343606 9 26.269548838072176 17.112791159029943 20.024212189781316 36.59344781311656 10-11 25.047323589069748 26.84347005570488 27.005746544641607 21.103459810583757 12-13 26.07045494250414 23.886445170036335 27.76171077774813 22.281389109711387 14-15 22.684480240703966 25.34920423208921 26.11267697178269 25.853638555424137 16-17 22.180669917496896 29.008903049625435 26.478895841733795 22.331531191143874 18-19 22.131974402929096 27.405141620696472 28.87144865014177 21.591435326232663 20-21 22.79242150647712 26.34320693257611 29.029248413473812 21.835123147472967 22-23 22.35225695896103 26.348841925025653 29.05277154986955 22.24612956614377 24-25 22.449890782503672 26.454938516287168 29.03107003358273 22.064100667626434 26-27 22.135006892666688 26.735015559280995 29.02577776092603 22.104199787126287 28-29 21.880510683935015 27.102620550786565 29.03584760729891 21.981021157979512 30-31 22.104203674419125 26.63288614257087 29.037336315282182 22.225573867727817 32-33 21.86386056238322 26.8067958335012 28.98392586240343 22.345417741712147 34-35 22.525691548405597 26.759190206321154 28.726192769297903 21.98892547597535 36-37 22.281625325785193 26.627368765988823 28.807755676422985 22.283250231803 38-39 22.05404318539784 26.941232343316766 28.894794619038095 22.109929852247298 40-41 22.214906950876806 26.78270529942327 28.917801699655342 22.08458605004458 42-43 22.091273312193156 26.829433412496172 28.621530758419738 22.45776251689093 44-45 22.224561940428366 26.92701185108649 28.409260950727568 22.439165257757576 46-47 22.320705614094948 27.20673658897221 28.096979963007797 22.375577833925046 48-49 22.35074749393775 27.29018933037502 27.963358731076525 22.3957044446107 50-51 22.345346061082903 27.311599197767183 27.978309650492456 22.364745090657454 52-53 22.56229931569275 27.49921791522607 27.59442234744607 22.344060421635106 54-55 22.221040988327115 27.238516290475644 27.88142729018211 22.65901543101513 56-57 22.38673457920896 27.565032081094294 27.691183419374845 22.35704992032191 58-59 22.628411644239662 27.268853625261748 27.721725781019167 22.381008949479423 60-61 22.46376541795386 27.23879978161601 28.20040691450023 22.097027885929904 62-63 22.306336848276185 27.383485297014587 28.090388886670002 22.219788968039225 64-65 22.07786976937191 27.510334246404717 27.93233501337114 22.47946097085223 66-67 22.168377959643205 27.595839007460228 27.612324017440937 22.623459015455627 68-69 22.060128138605503 27.438409156064886 27.932679338369116 22.568783366960492 70-71 22.205580005510548 27.39876398538554 27.831924217099353 22.56373179200456 72-73 22.273275218000204 27.283683100706124 27.88726920786686 22.555772473426806 74-75 22.079824636820717 27.73725716119345 27.628467542173745 22.554450659812087 76-77 22.167559057037266 27.427251594505307 27.63720057642878 22.767988772028648 78-79 22.223685346323165 27.530910377254703 27.632802490094292 22.612601786327836 80-81 22.218013822730708 27.72378502773313 27.616047744235818 22.44215340530035 82-83 22.24764485228978 27.52512558136071 27.730673130988155 22.496556435361356 84-85 22.259964718513032 27.498676605774737 27.58566185280607 22.65569682290616 86-87 22.25738232385218 27.50510929041281 27.688224634124715 22.549283751610297 88-89 22.147666454880596 27.472083335166857 27.447542328159635 22.932707881792908 90-91 22.132579180925497 27.386902525462798 27.748746746279163 22.731771547332542 92-93 22.359015118229006 27.216264942376604 27.86307681788055 22.561643121513843 94-95 22.1395115508155 27.29321269633441 27.697178972225032 22.870096780625055 96-97 22.01647518280467 27.28062001439894 27.714856198400817 22.988048604395573 98-99 21.986770838163977 27.302130402124952 27.539044847880888 23.172053911830183 100-101 21.92881185555234 26.975240009950703 28.110330962425937 22.985617172071017 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1926.0 1 1102.5 2 284.5 3 288.0 4 266.0 5 264.5 6 391.5 7 727.5 8 1209.0 9 1546.0 10 1527.5 11 1413.0 12 1486.0 13 1644.5 14 1762.0 15 1826.5 16 1954.5 17 2063.0 18 2180.5 19 2362.5 20 2563.5 21 2952.5 22 3469.5 23 4043.5 24 4884.0 25 6011.5 26 7430.5 27 9294.5 28 11154.0 29 13456.5 30 16433.0 31 19107.0 32 22036.0 33 24970.5 34 28108.5 35 31042.0 36 34322.5 37 38180.0 38 40770.0 39 42493.5 40 44288.5 41 46740.5 42 49216.0 43 52171.0 44 54523.0 45 55938.0 46 56399.5 47 57986.5 48 62566.0 49 62528.0 50 58426.0 51 55210.0 52 52167.5 53 50639.0 54 49057.5 55 45032.0 56 40038.0 57 35531.0 58 31974.5 59 28206.5 60 23614.0 61 19112.0 62 15017.5 63 11409.5 64 8701.5 65 6799.0 66 5009.5 67 3950.0 68 2902.0 69 1815.0 70 1287.5 71 935.5 72 735.0 73 585.0 74 461.5 75 370.0 76 241.0 77 127.5 78 85.0 79 56.0 80 35.5 81 21.0 82 15.5 83 10.5 84 10.5 85 8.5 86 5.5 87 5.0 88 4.5 89 5.0 90 4.5 91 4.0 92 3.5 93 1.5 94 0.5 95 1.0 96 1.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2978420639752321 2 0.26689567892517396 3 0.27034167376699264 4 0.26986869408282144 5 0.25736851671543987 6 0.20459749766719662 7 0.21121921324559334 8 0.2066921219828119 9 0.20520561440398816 10-11 0.21476656087687732 12-13 0.21466520808741205 14-15 0.2012190713516881 16-17 0.21118542898243825 18-19 0.19831362472035075 20-21 0.20338126419361358 22-23 0.20138799266746352 24-25 0.19463114003644646 26-27 0.20588129966708987 28-29 0.20395559666725 30-31 0.20895566761420262 32-33 0.2096989214036145 34-35 0.20172583529901436 36-37 0.2007123074043618 38-39 0.1947662770890668 40-41 0.19297571114184728 42-43 0.19290814261553713 44-45 0.20730023871960343 46-47 0.19669198008890665 48-49 0.20341504845676867 50-51 0.20953000008783906 52-53 0.21331383756120864 54-55 0.21490169792949765 56-57 0.18817834577382517 58-59 0.1777390084589038 60-61 0.18679319098446667 62-63 0.18662426966869125 64-65 0.18449586108992086 66-67 0.19456357151013629 68-69 0.1858472316161243 70-71 0.19000269598419978 72-73 0.20091501298329234 74-75 0.19027297008944044 76-77 0.1995636424570889 78-79 0.19773929224671433 80-81 0.18746887624756836 82-83 0.19922579982553806 84-85 0.18358368598473357 86-87 0.18702968082655227 88-89 0.19321220098393288 90-91 0.19081351829992182 92-93 0.19513790398377273 94-95 0.19429329740489562 96-97 0.1883472670896006 98-99 0.19348247508917357 100-101 0.18219853119537507 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1479979.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.091474976655434 #Duplication Level Percentage of deduplicated Percentage of total 1 81.63425185085872 50.687911060355816 2 10.809637488793472 13.42372671284273 3 3.189440149398351 5.941111296777237 4 1.380676141861035 3.4291287245291846 5 0.7583900827594433 2.354477942310081 6 0.4743197603705428 1.7670728119188457 7 0.31855817986479296 1.3845823077578585 8 0.21880280249062145 1.0868630988534802 9 0.1564864143907347 0.8744825054995954 >10 0.9565582349726102 10.89219021720181 >50 0.06950583944507513 2.9482556445090635 >100 0.031198983363849433 3.41906558896203 >500 0.0014131464300032176 0.5446224072790337 >1k 6.522214292322543E-4 0.8874328183052094 >5k 1.0870357153870904E-4 0.35907686289806157 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5320 0.3594645599701077 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3968 0.26811191239875704 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2690 0.18175933577435896 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2099 0.14182633672504813 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2075 0.14020469209360403 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 6.756852631017062E-5 0.0 0.0 0.0 0.0 7 6.756852631017062E-5 0.0 0.0 0.0 0.0 8 6.756852631017062E-5 0.0 0.0 0.0 0.0 9 6.756852631017062E-5 0.0 0.0 0.0 0.0 10-11 6.756852631017062E-5 0.0 0.0 0.0 0.0 12-13 6.756852631017062E-5 0.0 0.0 6.756852631017062E-5 0.0 14-15 6.756852631017062E-5 0.0 0.0 6.756852631017062E-5 0.0 16-17 6.756852631017062E-5 0.0 0.0 6.756852631017062E-5 0.0 18-19 6.756852631017062E-5 0.0 0.0 1.0135278946525592E-4 0.0 20-21 6.756852631017062E-5 0.0 0.0 2.0270557893051187E-4 0.0 22-23 6.756852631017062E-5 0.0 0.0 2.7027410524068247E-4 0.0 24-25 6.756852631017062E-5 0.0 0.0 4.0541115786102373E-4 0.0 26-27 6.756852631017062E-5 0.0 0.0 6.081167367915356E-4 0.0 28-29 6.756852631017062E-5 0.0 0.0 0.0013851547893584976 0.0 30-31 6.756852631017062E-5 0.0 0.0 0.0027027410524068244 0.0 32-33 6.756852631017062E-5 0.0 0.0 0.004628444052246688 0.0 34-35 6.756852631017062E-5 0.0 0.0 0.006486578525776379 6.756852631017062E-5 36-37 6.756852631017062E-5 0.0 0.0 0.00834471299930607 6.756852631017062E-5 38-39 6.756852631017062E-5 0.0 0.0 0.013142078367328185 6.756852631017062E-5 40-41 6.756852631017062E-5 0.0 0.0 0.02202733957711562 6.756852631017062E-5 42-43 6.756852631017062E-5 0.0 0.0 0.030574758155352204 6.756852631017062E-5 44-45 6.756852631017062E-5 0.0 0.0 0.041520859417599844 6.756852631017062E-5 46-47 6.756852631017062E-5 0.0 0.0 0.0602373412055171 1.0135278946525592E-4 48-49 6.756852631017062E-5 0.0 0.0 0.07354834088862072 1.3513705262034123E-4 50-51 6.756852631017062E-5 0.0 0.0 0.09324456630803546 1.3513705262034123E-4 52-53 6.756852631017062E-5 0.0 0.0 0.11419080946418834 1.3513705262034123E-4 54-55 6.756852631017062E-5 0.0 0.0 0.14331284430387187 1.3513705262034123E-4 56-57 6.756852631017062E-5 0.0 0.0 0.2161517156662358 1.3513705262034123E-4 58-59 6.756852631017062E-5 0.0 0.0 0.2710511432932494 1.3513705262034123E-4 60-61 6.756852631017062E-5 0.0 0.0 0.31747072086833666 1.3513705262034123E-4 62-63 6.756852631017062E-5 0.0 0.0 0.3751066738109122 1.3513705262034123E-4 64-65 6.756852631017062E-5 0.0 0.0 0.4311885506483538 1.3513705262034123E-4 66-67 6.756852631017062E-5 0.0 0.0 0.5268993681667105 1.3513705262034123E-4 68-69 6.756852631017062E-5 0.0 0.0 0.6516646519984406 1.3513705262034123E-4 70-71 6.756852631017062E-5 0.0 0.0 0.7157533992036373 1.3513705262034123E-4 72-73 6.756852631017062E-5 0.0 0.0 0.7980180799862701 1.3513705262034123E-4 74-75 6.756852631017062E-5 0.0 0.0 0.9493377946578971 1.3513705262034123E-4 76-77 6.756852631017062E-5 0.0 0.0 1.1225159275908645 1.3513705262034123E-4 78-79 6.756852631017062E-5 0.0 0.0 1.2927548296293394 1.3513705262034123E-4 80-81 6.756852631017062E-5 0.0 0.0 1.4563382318262623 1.3513705262034123E-4 82-83 6.756852631017062E-5 0.0 0.0 1.6915104876488112 1.3513705262034123E-4 84-85 6.756852631017062E-5 0.0 0.0 2.0259071243578455 1.3513705262034123E-4 86-87 6.756852631017062E-5 0.0 0.0 2.2886811231780992 1.3513705262034123E-4 88-89 6.756852631017062E-5 0.0 0.0 2.6717270988304564 1.3513705262034123E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7625 0.0 30.863045 1 GGTATCA 5170 0.0 27.954803 1 GTACATG 6500 0.0 25.891863 1 TACATGG 6465 0.0 25.654787 2 TCAACGC 9145 0.0 25.517534 4 ATCAACG 9170 0.0 25.497204 3 CAACGCA 9260 0.0 25.148455 5 ACATGGG 6450 0.0 25.124153 3 TATCAAC 9445 0.0 24.70535 2 AACGCAG 9490 0.0 24.628267 6 CATGGGG 3230 0.0 22.65996 4 GTTCGCG 105 0.0021953005 22.6212 7 GAGTACT 5155 0.0 22.438332 12-13 ACGCAGA 10555 0.0 21.963245 7 GTACCTG 1270 0.0 21.711893 1 CGCAGAG 10690 0.0 21.686615 8 GTACTTT 5405 0.0 21.091803 14-15 AGAGTAC 8490 0.0 20.757694 10-11 GCTTACG 115 0.003704953 20.670242 1 TACCTGG 1225 0.0 20.561333 2 >>END_MODULE