FastQCFastQC Report
Thu 26 May 2016
SRR937201_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937201_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479979
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71400.48243927785461815No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55880.3775729250212334No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39380.2660848566094519No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23330.15763737188162805No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15470.10452851020183394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA88700.029.8484421
GGTATCA61400.028.643331
TCAACGC106850.024.5861854
ATCAACG106950.024.5631983
TATCAAC107200.024.5059152
GTACATG56500.024.3130531
CAACGCA107950.024.291655
AACGCAG110600.023.7915026
GGACCGA2402.2919266E-1023.7485586
TACATGG58100.022.9758072
GAGTACT65700.022.26728212-13
ACATGGG56900.021.957563
GTACTTT66400.021.60260214-15
GTATACG1100.002875947421.5932454
ACGCAGA123750.021.1489227
AGTACTT67400.020.96568112-13
CGCAGAG125400.020.7570138
CGCGGAA1753.6379788E-1220.3572882-83
AGAGTAC96950.020.307610-11
GCAGAGT131900.019.5900699
Produced by FastQC (version 0.11.5)