##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937201_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1479979 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20777929957114 33.0 31.0 34.0 31.0 34.0 2 32.4250384633836 34.0 31.0 34.0 31.0 34.0 3 32.507636932686204 34.0 31.0 34.0 31.0 34.0 4 36.10324403251668 37.0 35.0 37.0 35.0 37.0 5 36.001664888488285 37.0 35.0 37.0 35.0 37.0 6 36.07896666101343 37.0 35.0 37.0 35.0 37.0 7 36.036272136293825 37.0 35.0 37.0 35.0 37.0 8 36.018274583625846 37.0 35.0 37.0 35.0 37.0 9 37.728854260769914 39.0 37.0 39.0 35.0 39.0 10-11 37.650260915864344 39.0 37.0 39.0 35.0 39.0 12-13 37.571777707656665 39.0 37.0 39.0 35.0 39.0 14-15 38.80588812408825 40.0 38.0 41.0 35.0 41.0 16-17 38.78301719146015 40.0 38.0 41.0 35.0 41.0 18-19 38.776011348809675 40.0 38.0 41.0 35.0 41.0 20-21 38.66902537130594 40.0 38.0 41.0 34.0 41.0 22-23 38.55260446263089 40.0 38.0 41.0 34.0 41.0 24-25 38.410400755686396 40.0 38.0 41.0 34.0 41.0 26-27 38.37350394836683 40.0 38.0 41.0 34.0 41.0 28-29 38.28009519053987 40.0 38.0 41.0 34.0 41.0 30-31 38.37315225418739 40.0 38.0 41.0 34.0 41.0 32-33 38.313305796906576 40.0 38.0 41.0 34.0 41.0 34-35 38.20254848210684 40.0 38.0 41.0 34.0 41.0 36-37 38.0534108929924 40.0 37.5 41.0 33.5 41.0 38-39 37.931709503986205 40.0 37.0 41.0 33.0 41.0 40-41 37.78212630044075 40.0 37.0 41.0 33.0 41.0 42-43 37.58601845026179 40.0 37.0 41.0 33.0 41.0 44-45 37.29878092864831 40.0 37.0 41.0 32.5 41.0 46-47 36.949309753719476 40.0 36.5 41.0 31.0 41.0 48-49 36.65238087837733 40.0 36.0 41.0 31.0 41.0 50-51 36.43431629773126 39.0 36.0 41.0 30.0 41.0 52-53 36.207962748120075 39.0 35.5 41.0 30.0 41.0 54-55 35.98178791726099 39.0 35.0 40.0 29.5 41.0 56-57 35.73354757060742 39.0 35.0 40.0 28.5 41.0 58-59 35.48527816948754 38.0 35.0 40.0 28.0 41.0 60-61 35.158230623542636 38.0 34.5 40.0 28.0 41.0 62-63 34.787789218630806 38.0 34.0 40.0 27.0 41.0 64-65 34.43872446838773 37.0 34.0 40.0 26.5 41.0 66-67 34.01635766453443 36.5 33.5 39.0 26.0 41.0 68-69 33.61127320049812 36.0 33.0 39.0 26.0 40.0 70-71 33.20104643376696 35.5 33.0 38.5 25.5 40.0 72-73 32.75277081634266 35.0 32.0 38.0 24.5 40.0 74-75 32.30384958164947 35.0 32.0 37.0 23.5 39.0 76-77 30.97657095134458 34.0 30.5 36.0 21.5 38.5 78-79 31.622491940764025 34.5 31.5 36.0 23.0 38.5 80-81 31.478165230722865 35.0 32.0 36.0 22.0 37.5 82-83 31.153300486020413 34.0 31.0 35.5 21.0 37.0 84-85 30.794653167376026 34.0 31.0 35.0 20.0 36.5 86-87 30.427533431217604 34.0 31.0 35.0 18.5 36.0 88-89 30.042618172284875 34.0 30.5 35.0 16.5 36.0 90-91 29.612985386954815 34.0 30.0 35.0 8.5 35.0 92-93 29.25237351340796 34.0 29.5 35.0 4.5 35.0 94-95 28.834122984177476 34.0 29.0 35.0 2.0 35.0 96-97 28.35036848495823 34.0 29.0 35.0 2.0 35.0 98-99 27.833088172197037 33.5 29.0 35.0 2.0 35.0 100-101 25.903167207102264 31.5 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19601528815743308 1101 2 0.14242535340719087 1101 3 0.1068479600004153 1101 4 0.051559244893404355 1101 5 -0.11214008185756086 1101 6 -0.039752916779377756 1101 7 0.0033586788783424026 1101 8 0.024061235401006797 1101 9 -0.017475437978880848 1101 10-11 0.060230552358881084 1101 12-13 0.021135312135221795 1101 14-15 0.08431135617642127 1101 16-17 0.02697073965814667 1101 18-19 0.014297778154521268 1101 20-21 -0.021914050057866064 1101 22-23 0.10838411584160923 1101 24-25 0.1943610612163056 1101 26-27 0.061147098193465865 1101 28-29 0.10629878621897149 1101 30-31 0.20621105457703237 1101 32-33 0.12751450490792848 1101 34-35 0.12000552942294718 1101 36-37 0.0463162515859139 1101 38-39 0.11034653511108417 1101 40-41 0.04841679787664788 1101 42-43 0.22472815786871791 1101 44-45 0.13357490175311426 1101 46-47 0.18826454447552265 1101 48-49 0.21617767330140936 1101 50-51 0.13144241712395655 1101 52-53 0.10815612520477202 1101 54-55 0.15882488768549052 1101 56-57 0.17849428750358953 1101 58-59 0.05285897942669493 1101 60-61 0.07158813599956204 1101 62-63 0.06626602074895516 1101 64-65 0.16062570458259984 1101 66-67 0.1364306499438399 1101 68-69 0.09819629432818289 1101 70-71 -0.11282421849459467 1101 72-73 -0.043843569891627965 1101 74-75 -0.025475747131928017 1101 76-77 0.011562276054963405 1101 78-79 0.11531247637108066 1101 80-81 0.08177880070962829 1101 82-83 0.08716373449584935 1101 84-85 -0.06410975493980331 1101 86-87 -0.02833703215371841 1101 88-89 0.06736509872200003 1101 90-91 0.1804358368221557 1101 92-93 0.06069833443557471 1101 94-95 0.026383367322612372 1101 96-97 0.1457674805607958 1101 98-99 0.12375835398830404 1101 100-101 0.48320825403578027 1102 1 0.06740577413693671 1102 2 0.1257324551962924 1102 3 0.056283178792909894 1102 4 0.06136341838562487 1102 5 -0.08414352943194814 1102 6 0.010175883314843759 1102 7 -0.0128288606028093 1102 8 0.049019211396469586 1102 9 -0.016911192109013484 1102 10-11 0.10059153052781511 1102 12-13 -0.0037993766730082257 1102 14-15 -0.09586883847234162 1102 16-17 -0.17344254099796075 1102 18-19 -0.03194816666286471 1102 20-21 -0.0699905819265112 1102 22-23 -0.05927078410379494 1102 24-25 -0.06678868342763877 1102 26-27 -0.1687009194512612 1102 28-29 -0.10273262739359268 1102 30-31 -0.007414626640112942 1102 32-33 -0.12313111228283447 1102 34-35 -0.11418648548085031 1102 36-37 -0.06992107429552163 1102 38-39 -0.17025416725189046 1102 40-41 0.05869661885974864 1102 42-43 0.06168197964411348 1102 44-45 -0.06042396333108968 1102 46-47 -0.14409527736410865 1102 48-49 -0.025024052627131255 1102 50-51 -0.04067639571130144 1102 52-53 -0.09906584719321643 1102 54-55 -0.16602361176311575 1102 56-57 -0.17307130741713195 1102 58-59 -0.1368017771124599 1102 60-61 -0.29379746770069204 1102 62-63 -0.3769127070746805 1102 64-65 -0.34358767029293347 1102 66-67 -0.23611429165082853 1102 68-69 -0.0031174242341123204 1102 70-71 -0.03398557996527529 1102 72-73 -0.01112293048436186 1102 74-75 -0.1642652416661292 1102 76-77 -0.2944241370757972 1102 78-79 -0.32853060546062807 1102 80-81 -0.27900691434085445 1102 82-83 -0.26475143590212014 1102 84-85 -0.3575676613413705 1102 86-87 -0.3553975467202548 1102 88-89 -0.28108376047349637 1102 90-91 -0.3003003059630984 1102 92-93 -0.27130066983641754 1102 94-95 -0.3778337549163844 1102 96-97 -0.19794310520564906 1102 98-99 -0.14879702669028916 1102 100-101 0.1341161866688907 1103 1 0.10136880214221122 1103 2 0.09223335515088138 1103 3 0.12724336742776643 1103 4 0.13836232968675688 1103 5 0.059727205357724245 1103 6 0.012099433676738158 1103 7 0.03887995895219376 1103 8 0.0891210785970884 1103 9 0.015590572239652545 1103 10-11 0.03974940280538419 1103 12-13 0.08491863937845778 1103 14-15 0.046874901509156075 1103 16-17 0.13330219467798798 1103 18-19 0.045704237501546174 1103 20-21 0.029665848782450155 1103 22-23 0.03588313822110223 1103 24-25 0.11357835580381703 1103 26-27 0.10233777586076798 1103 28-29 0.02649209865366231 1103 30-31 -0.015724025010889875 1103 32-33 -0.0356856156118468 1103 34-35 -0.0348816115018451 1103 36-37 -0.05544602957133549 1103 38-39 -0.13756119674523148 1103 40-41 0.12704393021120808 1103 42-43 0.11944484024141389 1103 44-45 0.0021907193323329466 1103 46-47 0.040123786354868685 1103 48-49 0.13498869445006534 1103 50-51 0.07150226032003815 1103 52-53 0.14432728251512827 1103 54-55 0.03956239818288765 1103 56-57 -0.10355323446883347 1103 58-59 -0.045939188416326715 1103 60-61 -0.10662086323757336 1103 62-63 -0.18091490635581664 1103 64-65 -0.18871095586575848 1103 66-67 -0.019736212246932894 1103 68-69 -0.007380309450233824 1103 70-71 -0.11570419233924412 1103 72-73 -0.04599193101502408 1103 74-75 0.06227931566016309 1103 76-77 0.0879873582712456 1103 78-79 -0.03285312292631204 1103 80-81 0.006166265371049917 1103 82-83 -0.17616636147122477 1103 84-85 -0.025597116390141395 1103 86-87 0.08256439805699856 1103 88-89 0.05358145094745126 1103 90-91 0.028538451716606517 1103 92-93 0.026636273162811364 1103 94-95 0.2377970065710322 1103 96-97 0.24957395016481243 1103 98-99 0.3500839112319909 1103 100-101 0.6608675420716281 1104 1 0.03598935277208426 1104 2 0.03632724655867037 1104 3 -0.03808630191625895 1104 4 0.010492690569520846 1104 5 -0.0735419969509934 1104 6 -0.014147757788009585 1104 7 -0.07434325289096932 1104 8 -0.013846775166236114 1104 9 -0.010417809640415499 1104 10-11 -0.032484610406505965 1104 12-13 -0.03667075613057591 1104 14-15 0.007171686885499184 1104 16-17 -0.11818952434739316 1104 18-19 -0.19286934051631732 1104 20-21 -0.2357833374710836 1104 22-23 -0.18262538941129947 1104 24-25 -0.2219973210475743 1104 26-27 -0.18135259461922715 1104 28-29 -0.24751022665656564 1104 30-31 -0.15245316336797288 1104 32-33 -0.14553606251090656 1104 34-35 -0.09062402901724198 1104 36-37 -0.1318095986646668 1104 38-39 -0.16588451801475657 1104 40-41 -0.21458271062366663 1104 42-43 -0.14294158505939691 1104 44-45 -0.09929830888083302 1104 46-47 -0.22193637796406307 1104 48-49 -0.3151477521530097 1104 50-51 -0.3902817593946466 1104 52-53 -0.22397259505290634 1104 54-55 -0.2088903847896475 1104 56-57 -0.37782576217298924 1104 58-59 -0.393763386442167 1104 60-61 -0.27244244669086726 1104 62-63 -0.31084918326730104 1104 64-65 -0.40675363663228836 1104 66-67 -0.3791925672726961 1104 68-69 -0.19660971688853834 1104 70-71 -0.24873430477034475 1104 72-73 -0.18977015651406504 1104 74-75 -0.30212589150995584 1104 76-77 -0.2539249010692366 1104 78-79 -0.25699469776901296 1104 80-81 -0.13265329773074086 1104 82-83 -0.18044138938840248 1104 84-85 -0.20896447728636147 1104 86-87 -0.29302113389415396 1104 88-89 -0.1982328852515991 1104 90-91 -0.18378737425062752 1104 92-93 -0.22161722152956287 1104 94-95 -0.10672253769507378 1104 96-97 -0.02991623061292259 1104 98-99 0.012083700451700707 1104 100-101 0.5797577726293888 1105 1 0.0043253155311120395 1105 2 0.06255576786999484 1105 3 0.05305508507120038 1105 4 0.05044525711388559 1105 5 -0.04882885518515678 1105 6 0.06300101926956359 1105 7 0.04513754797674352 1105 8 -0.012237333106149606 1105 9 0.0010234356694311941 1105 10-11 -0.12618157860948287 1105 12-13 -0.021092057592497326 1105 14-15 -0.018805460464406565 1105 16-17 0.10769219820321752 1105 18-19 0.1614563674825007 1105 20-21 0.12528503105676947 1105 22-23 0.05712893227471483 1105 24-25 0.14920839172572897 1105 26-27 0.10901611746385242 1105 28-29 0.17416456614641618 1105 30-31 0.13307392404140472 1105 32-33 0.06990180173807659 1105 34-35 0.042385556277345415 1105 36-37 -0.021635148378969404 1105 38-39 0.030315291524864563 1105 40-41 0.1147593447914872 1105 42-43 -0.08053045791800884 1105 44-45 -0.05259335120247499 1105 46-47 0.036014499347281514 1105 48-49 0.0533184709710639 1105 50-51 -0.054543777340477106 1105 52-53 0.12331334461214993 1105 54-55 -0.24006464824772422 1105 56-57 -0.09428404252858513 1105 58-59 -0.007299760740913541 1105 60-61 -0.10569912448070085 1105 62-63 -0.11992757821050049 1105 64-65 -0.12398550008205689 1105 66-67 -0.21794573944990958 1105 68-69 -0.0305138042615809 1105 70-71 -0.11064631674807401 1105 72-73 -0.10555274366136302 1105 74-75 0.0027226094579120286 1105 76-77 0.050268405831449314 1105 78-79 0.004414436544472267 1105 80-81 -0.016052755930655138 1105 82-83 0.10234815449273071 1105 84-85 0.11428005670848052 1105 86-87 0.04237368215849102 1105 88-89 0.06858387059065763 1105 90-91 0.161046452964964 1105 92-93 -0.022862803834915013 1105 94-95 -0.07753493941596545 1105 96-97 -0.0389643958100514 1105 98-99 0.19254555388543793 1105 100-101 0.4500569173740807 1106 1 -0.15051609457027126 1106 2 -0.022176660121203895 1106 3 0.036374466214041945 1106 4 0.0816245272593008 1106 5 -0.10674052231689757 1106 6 -0.14825142236377076 1106 7 0.003559245183204496 1106 8 0.06532676945795401 1106 9 -0.021868233322599906 1106 10-11 -0.28287940516825216 1106 12-13 -0.35319023450627185 1106 14-15 -0.1955533598846131 1106 16-17 -0.062432673901703595 1106 18-19 -0.15860610732604385 1106 20-21 -0.16002951332669824 1106 22-23 -0.11561895164306435 1106 24-25 -0.08872451374043067 1106 26-27 -0.14078777674754406 1106 28-29 -0.10235505993487948 1106 30-31 -0.07993610603332257 1106 32-33 0.035564685810875574 1106 34-35 0.03608032885664869 1106 36-37 0.1951698775100894 1106 38-39 0.004421963772408333 1106 40-41 -0.08326874561871023 1106 42-43 -0.12699435274949877 1106 44-45 -0.04993933452185928 1106 46-47 0.022354917292894072 1106 48-49 0.011908757145988602 1106 50-51 0.008605387078340243 1106 52-53 0.1142262054313008 1106 54-55 -0.1341434936264463 1106 56-57 0.05222403423947242 1106 58-59 0.002164862704965742 1106 60-61 0.229182126875763 1106 62-63 0.09617596254540217 1106 64-65 0.0017791574691798928 1106 66-67 0.011053113369975165 1106 68-69 0.2888227690804328 1106 70-71 -0.046277498343584966 1106 72-73 -0.07542686389824382 1106 74-75 -0.039297125140414835 1106 76-77 0.0910789505869758 1106 78-79 -0.0010218794571414946 1106 80-81 0.02892368036984294 1106 82-83 -0.07903691046246308 1106 84-85 -0.03979010730727239 1106 86-87 0.20362379842674372 1106 88-89 0.15589939162746091 1106 90-91 0.253852936082815 1106 92-93 0.2014624527303681 1106 94-95 -0.5052451524194019 1106 96-97 -0.14244236685150824 1106 98-99 0.08303946142892116 1106 100-101 0.3708576955962606 1107 1 0.02719735088511044 1107 2 0.09999731285922309 1107 3 -0.01196667890130243 1107 4 0.10128034933448049 1107 5 -0.03679979995681748 1107 6 0.06858756302359836 1107 7 0.05749959993000431 1107 8 -0.014029447801490846 1107 9 -0.005736903481590616 1107 10-11 -0.023567301501309146 1107 12-13 -0.08090253117018165 1107 14-15 -0.04269057153796041 1107 16-17 -0.02855366461947284 1107 18-19 -0.0071425469915169515 1107 20-21 0.03825189463212553 1107 22-23 0.06835655612277236 1107 24-25 0.09054209778424394 1107 26-27 0.007036412205678744 1107 28-29 -0.06888042525056903 1107 30-31 0.014298109571861062 1107 32-33 -0.024785925346186843 1107 34-35 0.0882733717754931 1107 36-37 -0.0914915047158118 1107 38-39 -0.11433205699774618 1107 40-41 -0.029826966370478658 1107 42-43 0.03962176709242016 1107 44-45 0.05019392528882349 1107 46-47 0.11568848038452728 1107 48-49 0.1266489005151641 1107 50-51 0.059450897601266206 1107 52-53 0.026733098180123704 1107 54-55 0.03678059423312874 1107 56-57 -0.019784512769078333 1107 58-59 0.04046901673492442 1107 60-61 -0.052223534368607716 1107 62-63 -0.040740451387144105 1107 64-65 0.1969691675785441 1107 66-67 0.17445261709206505 1107 68-69 0.02340024357726378 1107 70-71 0.13098217448060723 1107 72-73 0.1140961578828481 1107 74-75 0.00164235652486866 1107 76-77 0.09942262304355154 1107 78-79 0.06473379557272807 1107 80-81 0.1463416566587874 1107 82-83 0.3018207772500645 1107 84-85 0.3627992686692032 1107 86-87 0.156077617009597 1107 88-89 0.19418286741093382 1107 90-91 0.34817905500570845 1107 92-93 0.25090528886675045 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-0.4500355082188747 2316 84-85 -0.45839237092906515 2316 86-87 -0.7344546270540242 2316 88-89 -0.3405574393020281 2316 90-91 -0.4221461624748777 2316 92-93 -0.455055475955529 2316 94-95 -0.5533971956886354 2316 96-97 -0.5200164854412499 2316 98-99 -0.6774322933787715 2316 100-101 -0.8710243156216855 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 14.0 5 31.0 6 59.0 7 140.0 8 301.0 9 598.0 10 1069.0 11 1662.0 12 2545.0 13 3710.0 14 5021.0 15 7027.0 16 9766.0 17 13879.0 18 12417.0 19 7369.0 20 6170.0 21 6450.0 22 6580.0 23 7267.0 24 8363.0 25 9921.0 26 12188.0 27 15169.0 28 19046.0 29 24351.0 30 30773.0 31 39033.0 32 50744.0 33 66852.0 34 93590.0 35 137133.0 36 216501.0 37 343160.0 38 292530.0 39 28539.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.43653893348279 19.226907868094603 15.087100640840589 22.24945255758202 2 15.831035440367735 20.239070959790645 39.95637775941415 23.973515840427464 3 19.806091843195073 24.309128710610082 29.73312459163272 26.151654854562125 4 13.091064129964005 16.8180089041804 36.815049402728015 33.27587756312759 5 13.759610426734369 37.49568345955765 33.95466737127924 14.790038742428738 6 32.47471754666789 36.16132391067711 17.05618795942375 14.30777058323125 7 29.20595494936077 32.51269105845421 20.009337970336066 18.27201602184896 8 26.988491052913588 34.96786103046057 19.006215628735273 19.03743228789057 9 26.400915148120347 16.86415820765024 19.93987752528921 36.7950491189402 10-11 25.19674265648364 26.837340259557735 26.80771146077073 21.158205623187897 12-13 26.267162001728444 23.871323248697077 27.739180142992186 22.12233460658229 14-15 22.83072935494355 25.31083211315836 26.26902138476289 25.589417147135197 16-17 22.214673316310567 28.855274297810986 26.791190956087892 22.13886142979056 18-19 22.06693473353338 27.016464422805996 29.537919119122634 21.378681724537984 20-21 22.693903089165453 25.928374659370167 29.8469437742022 21.530778477262178 22-23 22.30638328637844 25.951837423347087 29.82293855975794 21.918840730516536 24-25 22.389901197822265 26.0836735693617 29.89025170990775 21.636173522908287 26-27 22.19716905180432 26.35848789514066 29.80583642772271 21.63850662533231 28-29 21.803716180490543 26.64744733462686 29.848672254530356 21.700164230352243 30-31 22.095786494267823 26.21314221350438 29.842450467202575 21.84862082502522 32-33 21.910648732177957 26.286217574708832 29.870727895463382 21.93240579764983 34-35 22.41825728608311 26.24739945634364 29.654035631586666 21.680307625986586 36-37 22.367580891350485 26.072903737147623 29.69751597826726 21.861999393234633 38-39 22.046697959903486 26.414800480277083 29.811740571994605 21.72676098782483 40-41 22.227916747467365 26.313211200969743 29.724779878633413 21.734092172929483 42-43 22.069159859953007 26.32138771135615 29.41510580224613 22.194346626444716 44-45 22.219639602994366 26.448719880484788 29.17129229536365 22.160348221157193 46-47 22.389236604032895 26.79916404219249 28.821152192024346 21.990447161750268 48-49 22.50393417744441 26.915246770393363 28.513884318628847 22.06693473353338 50-51 22.47869733286756 26.914740006446035 28.541452277363394 22.065110383323006 52-53 22.720795362636903 27.217345651526138 28.01742457156487 22.044434414272093 54-55 22.305451631408282 27.013356270595736 28.263103733228647 22.41808836476734 56-57 22.46447415807927 27.29268455836198 28.13536543423927 22.10747584931948 58-59 22.742552428108777 26.88565175586951 28.276414732911753 22.095381083109963 60-61 22.450250983290978 26.87588810381769 28.773989360659847 21.899871552231485 62-63 22.34747925477321 27.096566910746706 28.61496683398886 21.940987000491223 64-65 22.188389159575912 27.123324047165536 28.41452480068974 22.273761992568815 66-67 22.202071786153724 27.20048730421175 28.214116551653774 22.383324357980754 68-69 22.193118956417628 27.008254846859316 28.442802228950548 22.355823967772515 70-71 22.347377901983744 27.10356025321981 28.244083193072335 22.30497865172411 72-73 22.384420579224827 27.0231043974978 28.24829152865786 22.344183494619514 74-75 22.1862945352603 27.38559128203846 27.986241696672725 22.44187248602852 76-77 22.272714680411006 27.09146548701029 28.11935169350376 22.516468139074945 78-79 22.37278366787637 27.15379745253142 28.06090491824546 22.41251396134675 80-81 22.35008064303615 27.46069369903222 27.893368757259395 22.29585690067224 82-83 22.295020047649487 27.218957900611766 28.02701263670848 22.459009415030266 84-85 22.315542810500798 27.127044982175242 28.07318286120473 22.484229346119232 86-87 22.213896278258 27.178696454476718 28.07424970219172 22.53315756507356 88-89 22.045583079219366 27.157006957531156 27.894753912048753 22.902656051200726 90-91 22.143050455980042 26.97555030647008 28.14831658540195 22.733082652147925 92-93 22.12352636122077 26.789304494862222 28.32150030321612 22.765668840700894 94-95 21.97956862901433 26.7945693824034 28.252326553282174 22.973535435300096 96-97 21.938824807649297 26.776562370141736 28.240941256598912 23.04367156561005 98-99 21.952664820917885 26.78861671883525 28.03478431259935 23.223934147647515 100-101 21.52942543913296 26.766955603865682 28.35312554272867 23.35049341427269 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 212.0 1 168.5 2 170.5 3 236.0 4 198.0 5 182.0 6 381.0 7 920.5 8 1598.0 9 1918.0 10 1809.0 11 1643.5 12 1686.0 13 1851.0 14 2094.0 15 2238.5 16 2236.5 17 2354.0 18 2518.5 19 2709.0 20 2964.5 21 3271.5 22 3745.0 23 4361.5 24 5156.0 25 6202.0 26 7573.5 27 9349.5 28 11141.0 29 13374.0 30 16297.5 31 18966.0 32 21789.5 33 24577.5 34 27707.5 35 30590.5 36 33814.5 37 37636.0 38 40578.5 39 42613.5 40 44016.5 41 46596.0 42 49603.5 43 52315.0 44 54428.5 45 56059.5 46 56736.0 47 58579.5 48 63087.5 49 62845.5 50 58839.0 51 55590.5 52 52340.5 53 50355.0 54 48901.0 55 45113.0 56 39705.5 57 35242.0 58 31767.5 59 28204.0 60 23813.5 61 19210.5 62 15009.5 63 11346.0 64 8348.5 65 6293.0 66 4739.5 67 3728.0 68 2676.0 69 1578.0 70 1093.0 71 779.0 72 613.5 73 500.0 74 396.5 75 316.5 76 201.0 77 110.0 78 68.0 79 37.5 80 19.5 81 16.0 82 12.0 83 6.0 84 5.0 85 3.5 86 2.5 87 1.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05594673978482127 2 0.0 3 0.0 4 0.0 5 0.013919116419895147 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0025676039997864835 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.446065788771327E-4 24-25 1.0135278946525593E-4 26-27 0.0010473121578076447 28-29 0.003074367947112763 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.012838019998932417 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 6.756852631017062E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.378426315508531E-4 84-85 0.0010135278946525592 86-87 0.0 88-89 0.0 90-91 0.009763652051819654 92-93 7.77038052566962E-4 94-95 0.0 96-97 0.0 98-99 1.0135278946525593E-4 100-101 0.012702882946312076 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1479979.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.73876923004018 #Duplication Level Percentage of deduplicated Percentage of total 1 80.18467269830275 47.90133658110211 2 11.600188769124617 13.859619998072784 3 3.412378449647597 6.115538661871802 4 1.524293292880994 3.6423762104926296 5 0.8364705657523274 2.4984861047599716 6 0.527476650837338 1.8906483551163724 7 0.34616937046275537 1.447581249560798 8 0.25438905423441155 1.2157511204446148 9 0.18421859994818135 0.990449318716695 >10 1.0121215648683208 11.117182660770263 >50 0.07819551611551367 3.214589159532376 >100 0.03704641206837242 3.9641903669595218 >500 0.0016993255406074716 0.6791394731611515 >1k 4.5315347749532575E-4 0.6017168964500048 >5k 2.2657673874766288E-4 0.8613938429890521 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7140 0.48243927785461815 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5588 0.3775729250212334 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3938 0.2660848566094519 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2333 0.15763737188162805 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1547 0.10452851020183394 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.756852631017062E-5 0.0 14-15 0.0 0.0 0.0 6.756852631017062E-5 0.0 16-17 0.0 0.0 0.0 1.0135278946525592E-4 0.0 18-19 0.0 0.0 0.0 1.6892131577542655E-4 0.0 20-21 0.0 0.0 0.0 2.7027410524068247E-4 6.756852631017062E-5 22-23 0.0 0.0 0.0 3.378426315508531E-4 6.756852631017062E-5 24-25 0.0 0.0 0.0 4.729796841711943E-4 6.756852631017062E-5 26-27 0.0 0.0 0.0 6.756852631017062E-4 6.756852631017062E-5 28-29 0.0 0.0 0.0 0.0014527233156686682 6.756852631017062E-5 30-31 0.0 0.0 0.0 0.002871662368182251 6.756852631017062E-5 32-33 0.0 0.0 0.0 0.00489871815748737 6.756852631017062E-5 34-35 0.0 0.0 0.0 0.006891989683637403 6.756852631017062E-5 36-37 0.0 0.0 0.0 0.00871633989401201 6.756852631017062E-5 38-39 0.0 0.0 0.0 0.01361505805149938 6.756852631017062E-5 40-41 0.0 0.0 0.0 0.022669240577062242 6.756852631017062E-5 42-43 0.0 0.0 0.0 0.03155450178684968 6.756852631017062E-5 44-45 0.0 0.0 0.0 0.04280466141749309 6.756852631017062E-5 46-47 0.0 0.0 0.0 0.06135222188963492 1.3513705262034123E-4 48-49 0.0 0.0 0.0 0.07489971141482413 1.3513705262034123E-4 50-51 0.0 0.0 0.0 0.09449458404477362 1.3513705262034123E-4 52-53 0.0 0.0 0.0 0.11571110130616719 1.3513705262034123E-4 54-55 0.0 0.0 0.0 0.14550882140895244 1.3513705262034123E-4 56-57 0.0 0.0 0.0 0.21855039835024687 1.3513705262034123E-4 58-59 0.0 0.0 0.0 0.2734498259772605 1.3513705262034123E-4 60-61 0.0 0.0 0.0 0.31997075634181293 1.3513705262034123E-4 62-63 0.0 0.0 0.0 0.3781810417580249 1.3513705262034123E-4 64-65 0.0 0.0 0.0 0.43480346680594795 1.3513705262034123E-4 66-67 0.0 0.0 0.0 0.5321697132189038 1.3513705262034123E-4 68-69 0.0 0.0 0.0 0.6582525833136821 1.3513705262034123E-4 70-71 0.0 0.0 0.0 0.7236251325187723 1.3513705262034123E-4 72-73 0.0 0.0 0.0 0.8070046939855228 1.3513705262034123E-4 74-75 0.0 0.0 0.0 0.9596082106570432 1.3513705262034123E-4 76-77 0.0 0.0 0.0 1.1339687928004385 1.3513705262034123E-4 78-79 0.0 0.0 0.0 1.306065829312443 1.3513705262034123E-4 80-81 0.0 0.0 0.0 1.4723181882986176 1.3513705262034123E-4 82-83 0.0 0.0 0.0 1.7095174999104716 1.3513705262034123E-4 84-85 0.0 0.0 0.0 2.048576364934908 1.3513705262034123E-4 86-87 0.0 0.0 0.0 2.3152017697548413 1.3513705262034123E-4 88-89 0.0 0.0 0.0 2.702639699617359 1.3513705262034123E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8870 0.0 29.848442 1 GGTATCA 6140 0.0 28.64333 1 TCAACGC 10685 0.0 24.586185 4 ATCAACG 10695 0.0 24.563198 3 TATCAAC 10720 0.0 24.505915 2 GTACATG 5650 0.0 24.313053 1 CAACGCA 10795 0.0 24.29165 5 AACGCAG 11060 0.0 23.791502 6 GGACCGA 240 2.2919266E-10 23.748558 6 TACATGG 5810 0.0 22.975807 2 GAGTACT 6570 0.0 22.267282 12-13 ACATGGG 5690 0.0 21.95756 3 GTACTTT 6640 0.0 21.602602 14-15 GTATACG 110 0.0028759474 21.593245 4 ACGCAGA 12375 0.0 21.148922 7 AGTACTT 6740 0.0 20.965681 12-13 CGCAGAG 12540 0.0 20.757013 8 CGCGGAA 175 3.6379788E-12 20.35728 82-83 AGAGTAC 9695 0.0 20.3076 10-11 GCAGAGT 13190 0.0 19.590069 9 >>END_MODULE