FastQCFastQC Report
Thu 26 May 2016
SRR937200_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937200_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688580
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56750.33608120432552796No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42900.254059624062822No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30340.17967759892927787No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22880.1354984661668384No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21950.12999087991093108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA83250.030.1997241
GGTATCA56150.027.1698271
GTACATG72450.026.0425621
TACATGG72400.025.7269782
ACATGGG72250.024.6608983
ATCAACG100450.024.5962493
TATCAAC100400.024.5626342
TCAACGC101400.024.3196774
CAACGCA102000.024.1744635
AACGCAG103750.023.9463216
ACGCAGA115200.021.2350947
GAGTACT52850.021.16694812-13
CGCAGAG114900.021.1251758
AGTACTT55500.020.92657912-13
AGAGTAC89500.020.8581310-11
CTTAACG1150.003717937520.657973
GTACTTT54100.020.32666414-15
GTACCTG14500.019.9937111
GCAGAGT122100.019.646639
GAGTACA56550.019.4138181
Produced by FastQC (version 0.11.5)