FastQCFastQC Report
Thu 26 May 2016
SRR937200_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937200_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688580
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74690.44232431984270804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59160.3503535515048147No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43380.2569022492271613No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26230.1553376209596229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA88700.030.1171571
GGTATCA60800.029.6303581
GTACATG67600.025.5950491
TACATGG67150.025.323092
ACATGGG65650.024.8164163
ATCAACG107650.024.7089143
TCAACGC109050.024.4352554
TATCAAC110700.024.1568582
CAACGCA110250.024.0831285
TATTCCG2002.0561856E-823.7492395
AACGCAG113100.023.4762556
TAATCCG1252.2570023E-422.7992694
TACCTGG14500.020.9648442
ACGCAGA127200.020.7625267
AGTACTT69300.020.5627212-13
GAGTACT67750.020.47227512-13
CGCAGAG128350.020.4654738
AGAGTAC103200.020.43599510-11
CATGGGG34050.019.9479664
GTACTTT70150.019.70357314-15
Produced by FastQC (version 0.11.5)