##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937200_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1688580 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2729085977567 33.0 31.0 34.0 31.0 34.0 2 32.474876523469426 34.0 31.0 34.0 31.0 34.0 3 32.53526631844508 34.0 31.0 34.0 31.0 34.0 4 36.150966492555874 37.0 35.0 37.0 35.0 37.0 5 36.07781745608737 37.0 35.0 37.0 35.0 37.0 6 36.118410143434126 37.0 35.0 37.0 35.0 37.0 7 36.06433512181833 37.0 35.0 37.0 35.0 37.0 8 36.040636511151384 37.0 35.0 37.0 35.0 37.0 9 37.77056994634545 39.0 37.0 39.0 35.0 39.0 10-11 37.683562816093996 39.0 37.0 39.0 35.0 39.0 12-13 37.58696123369932 39.0 37.0 39.0 35.0 39.0 14-15 38.816590567222164 40.0 38.0 41.0 35.0 41.0 16-17 38.785191995641306 40.0 38.0 41.0 35.0 41.0 18-19 38.765900046192655 40.0 38.0 41.0 35.0 41.0 20-21 38.66649196366178 40.0 38.0 41.0 34.0 41.0 22-23 38.51993420507172 40.0 38.0 41.0 34.0 41.0 24-25 38.46330348576911 40.0 38.0 41.0 34.0 41.0 26-27 38.432944545120755 40.0 38.0 41.0 34.0 41.0 28-29 38.30692534555662 40.0 38.0 41.0 34.0 41.0 30-31 38.39929615416504 40.0 38.0 41.0 34.0 41.0 32-33 38.35896433689846 40.0 38.0 41.0 34.0 41.0 34-35 38.24005791848772 40.0 38.0 41.0 34.0 41.0 36-37 38.07754829501712 40.0 38.0 41.0 33.5 41.0 38-39 37.96537949045944 40.0 37.0 41.0 33.0 41.0 40-41 37.80039204538725 40.0 37.0 41.0 33.0 41.0 42-43 37.60014775728719 40.0 37.0 41.0 33.0 41.0 44-45 37.33197449928342 40.0 37.0 41.0 32.5 41.0 46-47 36.99773448696538 40.0 36.5 41.0 31.5 41.0 48-49 36.69432659394284 40.0 36.0 41.0 31.0 41.0 50-51 36.45484223430338 39.0 36.0 41.0 30.0 41.0 52-53 36.215848227504765 39.0 35.5 41.0 30.0 41.0 54-55 35.99344478792832 39.0 35.0 40.0 29.5 41.0 56-57 35.72347771500314 39.0 35.0 40.0 28.5 41.0 58-59 35.455202300157524 38.0 35.0 40.0 28.0 41.0 60-61 35.12859503251252 38.0 34.0 40.0 28.0 41.0 62-63 34.76433749067263 37.5 34.0 40.0 27.0 41.0 64-65 34.36435436875955 37.0 34.0 40.0 26.0 41.0 66-67 33.94360971940921 36.5 33.5 39.0 26.0 41.0 68-69 33.51379798410498 36.0 33.0 39.0 26.0 40.0 70-71 33.10125371614019 35.5 32.5 38.5 25.0 40.0 72-73 32.6246582927667 35.0 32.0 37.5 24.0 40.0 74-75 32.15528728280567 35.0 31.5 37.0 22.5 39.0 76-77 30.89909746651032 34.0 30.5 35.5 21.5 38.5 78-79 31.502425706806903 34.5 31.0 36.0 22.0 38.5 80-81 31.309858283291284 34.5 31.0 36.0 21.5 37.5 82-83 30.966523350981298 34.0 31.0 35.0 20.0 37.0 84-85 30.612889232372765 34.0 31.0 35.0 19.0 36.5 86-87 30.208988617655073 34.0 30.5 35.0 17.5 36.0 88-89 29.76082211088607 34.0 30.0 35.0 12.0 36.0 90-91 29.269312973030594 34.0 29.0 35.0 7.0 35.0 92-93 28.911521219012425 34.0 29.0 35.0 2.0 35.0 94-95 28.47059067382061 34.0 29.0 35.0 2.0 35.0 96-97 28.002863648746285 33.5 29.0 35.0 2.0 35.0 98-99 27.24472841085409 33.0 27.0 35.0 2.0 35.0 100-101 24.724828554169775 31.0 21.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.11409804777105848 1101 2 -0.10811842189288967 1101 3 -0.065761871678788 1101 4 -0.076050880932101 1101 5 -0.10971897996568458 1101 6 -0.10368803714735009 1101 7 -0.10915451596139292 1101 8 -0.10678139725956015 1101 9 -0.16487905460312646 1101 10-11 -0.1344068234324851 1101 12-13 -0.10506865459806392 1101 14-15 -0.25598233287674077 1101 16-17 -0.21119683231445663 1101 18-19 -0.12121137876889065 1101 20-21 -0.08506785053398147 1101 22-23 -0.13969767281384549 1101 24-25 -0.12613332947019273 1101 26-27 -0.1637624314040167 1101 28-29 -0.2234639890328154 1101 30-31 -0.19268963812562134 1101 32-33 -0.09904916661434271 1101 34-35 -0.1883971257739887 1101 36-37 -0.08343573878376986 1101 38-39 -0.08795367199207504 1101 40-41 0.007774771333174613 1101 42-43 -0.0962866947751948 1101 44-45 -0.11306686255616683 1101 46-47 -0.023499687057118024 1101 48-49 -0.09520264364053332 1101 50-51 -0.15297439673368274 1101 52-53 -0.1569634134166904 1101 54-55 -0.1314726619470079 1101 56-57 -0.08476616919256941 1101 58-59 -0.0052666799476384085 1101 60-61 -0.001467929621604469 1101 62-63 0.10523793839737294 1101 64-65 -0.011620222041642592 1101 66-67 -0.03425144194656582 1101 68-69 -0.16561109569327215 1101 70-71 -0.16613480890752186 1101 72-73 -0.1291341176254477 1101 74-75 -0.08343833424559932 1101 76-77 -0.05487726061578613 1101 78-79 -0.21086964066372005 1101 80-81 -0.2958738539363388 1101 82-83 -0.1747756629095214 1101 84-85 -0.01784431849958068 1101 86-87 -0.18936423466728058 1101 88-89 -0.21737036474740634 1101 90-91 -0.48512559750793827 1101 92-93 -0.33296711818907454 1101 94-95 -0.20360358506988163 1101 96-97 -0.17558077213781687 1101 98-99 -0.18726895698638657 1101 100-101 0.4213310522798217 1102 1 -0.0543248149104798 1102 2 0.0051934862396052495 1102 3 -0.002316752140231415 1102 4 -0.07027794702781875 1102 5 -0.03268277987599788 1102 6 -0.1275363959652367 1102 7 -0.1011468837831444 1102 8 -0.1386510171454347 1102 9 -0.16238584706591297 1102 10-11 -0.19916954885871263 1102 12-13 -0.10225033209457024 1102 14-15 -0.06337512627156627 1102 16-17 -0.12849145373907334 1102 18-19 -0.1041761458907331 1102 20-21 -0.09076164894951688 1102 22-23 -0.04472519437826605 1102 24-25 -0.13276048701136745 1102 26-27 -0.18875144036390878 1102 28-29 -0.20494936215664694 1102 30-31 -0.15637502180084795 1102 32-33 -0.1260842676000209 1102 34-35 -0.029895995895060423 1102 36-37 -0.054987190040705514 1102 38-39 -0.07765396831051419 1102 40-41 0.06454217454607658 1102 42-43 0.04720287252953881 1102 44-45 0.012633266698472312 1102 46-47 0.1114067572095152 1102 48-49 0.15739168146563998 1102 50-51 0.17003886285709768 1102 52-53 0.28747577975303784 1102 54-55 0.2824160559701383 1102 56-57 0.23778744510105554 1102 58-59 0.35240264801620924 1102 60-61 0.24631429830618146 1102 62-63 0.1310213797359907 1102 64-65 0.11862426265442849 1102 66-67 0.29141549436694447 1102 68-69 0.2496532608033064 1102 70-71 0.2420911053271979 1102 72-73 0.27975323740272984 1102 74-75 0.32685103785825476 1102 76-77 0.3274999255020923 1102 78-79 0.18289404318120717 1102 80-81 0.13968633739569114 1102 82-83 0.2769411375547399 1102 84-85 0.14401589305595763 1102 86-87 0.19074330166909803 1102 88-89 0.17981065328669743 1102 90-91 0.1727897099334328 1102 92-93 0.3077423754317259 1102 94-95 0.34879290131442175 1102 96-97 0.43641221118218 1102 98-99 0.43080426971920005 1102 100-101 1.071781904744892 1103 1 -0.03148915147807685 1103 2 0.03955921093082537 1103 3 -0.02982044194048683 1103 4 0.0049257823350430385 1103 5 0.004532717104417827 1103 6 -0.040043870407615145 1103 7 -0.05002079468237497 1103 8 -0.021964810043314742 1103 9 -0.11724157000481483 1103 10-11 -0.050517567928253015 1103 12-13 -0.03038164924034703 1103 14-15 -0.1420331319187227 1103 16-17 -0.11932430985221743 1103 18-19 -0.13072373934609516 1103 20-21 -0.19123983261034283 1103 22-23 -0.1604270783893682 1103 24-25 -0.02873973380435757 1103 26-27 -0.1419061066740923 1103 28-29 -0.2984860244093994 1103 30-31 -0.21136456209780619 1103 32-33 -0.11283869761846432 1103 34-35 -0.08879076297247224 1103 36-37 -0.04336296435680964 1103 38-39 -0.12812497108712506 1103 40-41 -0.23832900490738496 1103 42-43 -0.1519824358329842 1103 44-45 -0.24471381659797942 1103 46-47 -0.0837857085525684 1103 48-49 -0.21225133374699823 1103 50-51 -0.054802525411048464 1103 52-53 -0.04143920219087249 1103 54-55 -0.21507695734565146 1103 56-57 -0.28042716126906697 1103 58-59 -0.24820266890918674 1103 60-61 -0.19992729915602325 1103 62-63 -0.21174683758015078 1103 64-65 -0.23623177642402737 1103 66-67 -0.15777207213527333 1103 68-69 -0.10162520756376381 1103 70-71 -0.08482542299078233 1103 72-73 -0.079595822217577 1103 74-75 0.03388631722626201 1103 76-77 0.11602265954923396 1103 78-79 0.02600165889280248 1103 80-81 -0.16560624524770873 1103 82-83 -0.041382491799627275 1103 84-85 -0.12229961783459586 1103 86-87 -0.09114298564214351 1103 88-89 -0.121799129335745 1103 90-91 -0.08346036910145571 1103 92-93 0.07467119706217318 1103 94-95 0.1741519906879745 1103 96-97 0.06282377430260411 1103 98-99 0.3020446929487761 1103 100-101 1.0060138120780664 1104 1 -0.03289588358131823 1104 2 -0.008493983281312012 1104 3 -0.0865501368793673 1104 4 -0.060673549762348955 1104 5 -0.07777787461083108 1104 6 -0.09569644016103496 1104 7 -0.12482834296412193 1104 8 -0.1108495958979816 1104 9 -0.03788138519828266 1104 10-11 -0.07584016243524871 1104 12-13 -0.08619359196619314 1104 14-15 -0.24439095469975314 1104 16-17 -0.2418025572122957 1104 18-19 -0.03192430185005435 1104 20-21 -0.14980829026718112 1104 22-23 -0.2371390746645332 1104 24-25 -0.17533807708098692 1104 26-27 -0.28719617492821925 1104 28-29 -0.38721427058893454 1104 30-31 -0.3177173340542083 1104 32-33 -0.25551599355731724 1104 34-35 -0.19473294293029397 1104 36-37 -0.19197412924809498 1104 38-39 -0.2229523618942082 1104 40-41 -0.21759469924938202 1104 42-43 -0.1524315134530525 1104 44-45 -0.11742581098427252 1104 46-47 -0.10853951862909383 1104 48-49 -0.053426840458605795 1104 50-51 -0.11602205211818273 1104 52-53 -0.09882149993831035 1104 54-55 -0.0823067575887535 1104 56-57 -0.0724567654479884 1104 58-59 -0.19304852337025125 1104 60-61 -0.11611482377029603 1104 62-63 0.015621728119761258 1104 64-65 -0.1709393848661236 1104 66-67 -0.252366188132342 1104 68-69 -0.05094696341475924 1104 70-71 -0.15071587638445294 1104 72-73 -0.10706747271713368 1104 74-75 -0.0761008668256693 1104 76-77 0.22004103064884717 1104 78-79 0.015727414604022272 1104 80-81 0.017928142009253634 1104 82-83 -0.09898673116791556 1104 84-85 -0.07152362492917419 1104 86-87 0.01244944643191559 1104 88-89 -0.12284910208886757 1104 90-91 -0.17827217677345786 1104 92-93 0.07559839359950615 1104 94-95 0.261822472546843 1104 96-97 0.052290755528233035 1104 98-99 0.36571714678255773 1104 100-101 0.9659915990273333 1105 1 -0.03253114121204703 1105 2 -0.035102214532287235 1105 3 0.029643897439463274 1105 4 -0.003672436136596957 1105 5 -0.11071365552592027 1105 6 -0.04313569965977848 1105 7 -0.1063577223273171 1105 8 -0.03133935173853075 1105 9 -0.08394405957650264 1105 10-11 -0.0925397588955903 1105 12-13 -0.06489136830703757 1105 14-15 -0.1760273858285899 1105 16-17 -0.08173454222025356 1105 18-19 -0.04374897165403979 1105 20-21 -0.0960560899582461 1105 22-23 -0.1297959701633289 1105 24-25 -0.08887629360336291 1105 26-27 -0.04616193991726192 1105 28-29 -0.13914826730792385 1105 30-31 -0.23036560793429572 1105 32-33 -0.18692407153505997 1105 34-35 -0.11310369648371932 1105 36-37 -0.14332281385252088 1105 38-39 -0.04318742074872972 1105 40-41 -0.14563579563188966 1105 42-43 -0.14149762450428938 1105 44-45 -0.2423723449738091 1105 46-47 -0.245098774296018 1105 48-49 -0.17940485533090111 1105 50-51 -0.22629602800159887 1105 52-53 -0.3000597799253768 1105 54-55 -0.2446690811149992 1105 56-57 -0.23658232974491256 1105 58-59 -0.22774634760897783 1105 60-61 -0.1790210277434241 1105 62-63 -0.1611718679334544 1105 64-65 -0.03329069187956435 1105 66-67 -0.11221411235789702 1105 68-69 -0.12202382754043839 1105 70-71 -0.07231818027256764 1105 72-73 0.04526527386714463 1105 74-75 0.039072343889088756 1105 76-77 0.03990595862240909 1105 78-79 -0.13178357197260127 1105 80-81 -0.12677842359288505 1105 82-83 -0.1718998803338927 1105 84-85 -0.2207751007826566 1105 86-87 -0.31729238398563453 1105 88-89 -0.06227528546591188 1105 90-91 0.12432089479795394 1105 92-93 0.2070820304856653 1105 94-95 0.290076714502991 1105 96-97 0.32599374076371035 1105 98-99 0.5242060883591151 1105 100-101 0.835226669856322 1106 1 -0.10876103516169877 1106 2 -0.015799116830585547 1106 3 0.004171183646469956 1106 4 -0.03789736602732319 1106 5 -0.009782088623943253 1106 6 -0.018044638189259388 1106 7 -0.0649561815350026 1106 8 -0.07372854685183228 1106 9 -0.1385899674513098 1106 10-11 -0.15724658931173252 1106 12-13 -0.08487202781771686 1106 14-15 -0.04065620960449223 1106 16-17 0.01249490045130841 1106 18-19 0.05552318126476763 1106 20-21 -0.03814099470633181 1106 22-23 -0.024001559564744923 1106 24-25 0.030935458967306317 1106 26-27 0.028051385679866314 1106 28-29 0.011149253886024724 1106 30-31 -0.04830197772444933 1106 32-33 -0.07564018538607797 1106 34-35 0.02700138684155462 1106 36-37 0.032657587784967745 1106 38-39 -0.08858599599934536 1106 40-41 0.04598397034818902 1106 42-43 -0.029938458724068084 1106 44-45 0.25029051806329505 1106 46-47 0.14234924813894878 1106 48-49 0.16115215076135314 1106 50-51 0.3278096355383582 1106 52-53 0.3246051460972552 1106 54-55 0.30304777412144546 1106 56-57 0.18233009007264656 1106 58-59 0.22975615053755405 1106 60-61 0.283841364675105 1106 62-63 0.28019679402702735 1106 64-65 0.4249970301331345 1106 66-67 0.43144962100122086 1106 68-69 0.46748073359162134 1106 70-71 0.5537444184445164 1106 72-73 0.5583852171350472 1106 74-75 0.4363214816256047 1106 76-77 0.4901390115186466 1106 78-79 0.6157721125195543 1106 80-81 0.28059094751887415 1106 82-83 0.1712423044967153 1106 84-85 0.2391681671153556 1106 86-87 0.4862424127780578 1106 88-89 0.4964411507265929 1106 90-91 0.510943723022173 1106 92-93 0.849458976622401 1106 94-95 0.7579353999944054 1106 96-97 0.7903337465658531 1106 98-99 0.907777632052504 1106 100-101 1.6427688160059546 1107 1 -0.011916620739810924 1107 2 9.983246673286317E-4 1107 3 0.02229381614178294 1107 4 0.022127398054699654 1107 5 -0.05478856507020424 1107 6 -0.0866020012902311 1107 7 -0.13059921177789846 1107 8 -0.12833538646837894 1107 9 0.02127917163666382 1107 10-11 -0.015013907519318082 1107 12-13 -0.059162099214937314 1107 14-15 0.06669431904477108 1107 16-17 0.0268345543793842 1107 18-19 0.09582815188630178 1107 20-21 0.013150149076210482 1107 22-23 0.11930731323459298 1107 24-25 0.01261302844469725 1107 26-27 -0.1476179716908419 1107 28-29 -0.049861180464013444 1107 30-31 -0.021924016941952118 1107 32-33 0.05170305811063969 1107 34-35 0.0062911660171636186 1107 36-37 -0.010696545383808598 1107 38-39 -0.042522900597830926 1107 40-41 -0.012457088554171492 1107 42-43 -0.006344894123380129 1107 44-45 0.0440208015997996 1107 46-47 -0.12730904286755873 1107 48-49 -0.12762626778968666 1107 50-51 -0.1111396986720905 1107 52-53 -0.03129698277111714 1107 54-55 -0.09558816261189662 1107 56-57 -0.13913025681478786 1107 58-59 -0.22505741887458441 1107 60-61 -0.09286952486105093 1107 62-63 -0.1528855458661127 1107 64-65 -0.18665975763106957 1107 66-67 -0.0987170268635964 1107 68-69 0.006241414970851622 1107 70-71 0.1520754932899422 1107 72-73 0.09622342966306263 1107 74-75 0.107711491452406 1107 76-77 0.06659383337899882 1107 78-79 -0.058123119918949584 1107 80-81 -0.11287144356061773 1107 82-83 -0.08441136804927041 1107 84-85 -0.020537056040012658 1107 86-87 -0.005953639376024 1107 88-89 0.16876898802847862 1107 90-91 0.3599453445122833 1107 92-93 0.22239504261283827 1107 94-95 0.13440843473615516 1107 96-97 0.22615916386510193 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0.15007915414435047 2315 46-47 -0.06132929052652969 2315 48-49 -0.07953355545959795 2315 50-51 0.2313406563017537 2315 52-53 0.3260920862264882 2315 54-55 0.1184111419652325 2315 56-57 0.08512318529393781 2315 58-59 0.094628408099112 2315 60-61 0.16198855326615558 2315 62-63 -0.013789247217495415 2315 64-65 0.022097076841532726 2315 66-67 0.13956814609102253 2315 68-69 0.13443739558744738 2315 70-71 0.28575513113407425 2315 72-73 0.2688011907363972 2315 74-75 0.4969483150983933 2315 76-77 0.3960306547471042 2315 78-79 0.16425341739772392 2315 80-81 0.03613496598717347 2315 82-83 -0.028675703776382733 2315 84-85 -0.17137045804468087 2315 86-87 -0.3449876983476159 2315 88-89 -0.049363325887281206 2315 90-91 -0.01581447867075525 2315 92-93 0.046844650248285546 2315 94-95 0.16592589701884464 2315 96-97 0.310695585465556 2315 98-99 0.6408236583100191 2315 100-101 0.4708546788176662 2316 1 -0.7243700869895555 2316 2 -0.4739026288852024 2316 3 -0.15826107309732862 2316 4 -0.05151389520226246 2316 5 0.11617852780825899 2316 6 0.05532745859246546 2316 7 0.20031437847591604 2316 8 0.14105591376998916 2316 9 0.20871951642499198 2316 10-11 0.22880790697293918 2316 12-13 0.10360524384844894 2316 14-15 0.20331576291391684 2316 16-17 0.19240949636211013 2316 18-19 0.11079413633244428 2316 20-21 0.17850705640682918 2316 22-23 0.07685643189484637 2316 24-25 0.26832614496542107 2316 26-27 0.29111250096102737 2316 28-29 0.5026303935170446 2316 30-31 0.3216176820957628 2316 32-33 0.4098874488344606 2316 34-35 0.2062285839856557 2316 36-37 0.4000460111852604 2316 38-39 0.31311023408171224 2316 40-41 0.382330988208345 2316 42-43 0.3091247539493551 2316 44-45 0.2328721062633008 2316 46-47 0.32899058328715824 2316 48-49 -0.07381109100261085 2316 50-51 -0.03547666209740896 2316 52-53 0.1736036772043832 2316 54-55 0.30561672050505706 2316 56-57 0.35016313190197224 2316 58-59 0.44362084718783024 2316 60-61 0.21781732367539064 2316 62-63 0.46428570334713726 2316 64-65 0.5041613773377449 2316 66-67 0.3827919161373501 2316 68-69 0.19246663724805302 2316 70-71 0.13213373610965817 2316 72-73 0.26874719687222637 2316 74-75 0.3887817873995374 2316 76-77 0.268401551877286 2316 78-79 0.06548713293680564 2316 80-81 0.1644535970568377 2316 82-83 0.22821559506814637 2316 84-85 0.3237063637171502 2316 86-87 0.2299920760819525 2316 88-89 0.3268791968476741 2316 90-91 0.35818066011118077 2316 92-93 0.39111483908518707 2316 94-95 0.17605018912388104 2316 96-97 0.37467477392892334 2316 98-99 0.4585474540255632 2316 100-101 0.110508480782336 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 13.0 5 37.0 6 62.0 7 142.0 8 337.0 9 707.0 10 1181.0 11 1905.0 12 2902.0 13 4192.0 14 5626.0 15 7726.0 16 10651.0 17 15306.0 18 13983.0 19 8556.0 20 7471.0 21 7688.0 22 7971.0 23 8683.0 24 10104.0 25 12164.0 26 14968.0 27 18215.0 28 23189.0 29 29150.0 30 36499.0 31 46421.0 32 60097.0 33 79408.0 34 110306.0 35 160303.0 36 250136.0 37 388506.0 38 315405.0 39 28557.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.299239117615855 19.248979040324574 15.06575567017091 22.38602617188866 2 15.783972331781735 20.28047234954814 39.853427139963756 24.08212817870637 3 19.886058107996067 24.186180103992704 29.555425268568857 26.372336519442374 4 13.050906678984708 16.70675952575537 36.76088784659299 33.481445948666924 5 13.807992770132499 37.5231781568931 33.853819043665254 14.815010029309153 6 32.50879437160217 36.1488351158962 17.024659773300645 14.317710739200987 7 29.365087825273307 32.419251678925484 19.913063047057292 18.302597448743914 8 27.023238460718474 34.979923959776855 18.964751447962193 19.03208613154248 9 26.329045707043786 16.88691089554537 19.907081689940657 36.87696170747018 10-11 25.300682172030058 26.818942813978868 26.746692629355962 21.13368238463511 12-13 26.29448577988959 23.75813523215135 27.744450807793243 22.202928180165816 14-15 22.827908656978053 25.264097644174395 26.15099669544825 25.756997003399306 16-17 22.28893508154781 28.865111513816345 26.64522853521894 22.200724869416906 18-19 22.15091970768338 27.04805220954885 29.274005377299268 21.5270227054685 20-21 22.776800625377536 25.992638785251515 29.595162799512014 21.635397789858935 22-23 22.479198374956326 25.94026306282831 29.545828718975226 22.034709843240137 24-25 22.46133701614269 26.10670829567811 29.627269529292903 21.804685158886297 26-27 22.28164119547026 26.40224828760836 29.51893135864937 21.79717915827201 28-29 21.90803157291287 26.667183880000188 29.61769840856221 21.807086138524735 30-31 22.194068963475118 26.220185659505795 29.571502598344757 22.014242778674326 32-33 22.0459202406756 26.33280626325078 29.570941264257545 22.050332231816082 34-35 22.581281313292827 26.30346208056473 29.320642196401707 21.794614409740728 36-37 22.467944940686817 26.112725552076625 29.39619644683488 22.023133060401676 38-39 22.163540106853695 26.459984939924038 29.50297307693105 21.87350187629122 40-41 22.339720859717723 26.329617681154698 29.432258809560118 21.898402649567462 42-43 22.202601190134217 26.349414024441163 29.19346995731414 22.25451482811048 44-45 22.364682698898925 26.417028534372868 28.939536384915705 22.278752381812502 46-47 22.450313280981653 26.816852029515925 28.559558919328666 22.173275770173756 48-49 22.62054507337526 26.878619905482715 28.367740942093356 22.133094079048668 50-51 22.542965094931837 26.889102085776212 28.37443295550107 22.193499863790876 52-53 22.84360231673951 27.122967226900705 27.84792547584361 22.185504980516175 54-55 22.400715393999693 26.984330028781578 28.128397825391747 22.486556751826978 56-57 22.57008847670824 27.215352544741734 27.97770908100297 22.23684989754705 58-59 22.874663918795672 26.879419393810185 28.010399270392877 22.23551741700127 60-61 22.61512631915574 26.903492875670683 28.519495670918758 21.961885134254818 62-63 22.47687405986095 27.02113610252401 28.39516043065771 22.106829406957328 64-65 22.317190277389958 27.076012707722676 28.2456052557786 22.361191759108763 66-67 22.35567755155219 27.19577988605811 27.963821672648027 22.484720889741677 68-69 22.3112023120018 26.952646602470715 28.26060950621232 22.475541579315163 70-71 22.433405583389593 27.059333878169824 28.08661715761172 22.42064338082886 72-73 22.478384204479504 26.9311492496654 28.14272939392863 22.447737151926468 74-75 22.29299174454275 27.37211147828353 27.79175401816911 22.54314275900461 76-77 22.431184782479953 27.036000663279204 27.90388965876654 22.628924895474302 78-79 22.43305025524405 27.068128249772 27.940695732508974 22.55812576247498 80-81 22.413188126469617 27.35923893426398 27.82400828625481 22.4035646530116 82-83 22.437285155446116 27.149156197043013 27.859962435792774 22.5535962117181 84-85 22.39067473225181 27.15123149806627 27.835418476009483 22.62267529367244 86-87 22.37652346942401 27.096998661597315 27.899211171516896 22.627266697461774 88-89 22.24140994208151 27.141621954541684 27.663983939167817 22.95298416420898 90-91 22.323594560659544 26.977624315936605 27.88032148018289 22.81845964322096 92-93 22.322164364618388 26.75444505806077 28.096734886654534 22.826655690666303 94-95 22.12717194328963 26.768083241540232 28.06263250778761 23.042112307382535 96-97 22.097857371282377 26.74477963732841 28.06899880372858 23.088364187660638 98-99 22.064216162948945 26.714874882889628 27.840111620546992 23.380797333614435 100-101 21.777082575094177 26.644896503872168 28.218282850029595 23.359738071004056 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 216.0 1 155.0 2 162.5 3 258.0 4 218.0 5 192.0 6 389.5 7 945.0 8 1674.5 9 2036.5 10 1851.5 11 1638.0 12 1757.0 13 1964.0 14 2196.5 15 2368.0 16 2420.5 17 2548.5 18 2709.5 19 2824.0 20 3086.5 21 3495.0 22 4063.5 23 4792.0 24 5672.5 25 6842.0 26 8401.0 27 10394.5 28 12212.5 29 14568.5 30 18041.5 31 21107.0 32 24150.5 33 27413.0 34 30875.0 35 34229.5 36 37886.0 37 42318.0 38 45683.0 39 47782.5 40 50023.5 41 53053.5 42 56119.5 43 59537.0 44 62128.0 45 64112.5 46 65139.0 47 67036.0 48 71833.5 49 71768.5 50 67613.0 51 64146.0 52 60678.5 53 58487.5 54 56698.5 55 52328.0 56 46300.5 57 41269.5 58 37229.0 59 33039.5 60 27896.5 61 22466.0 62 17710.5 63 13364.5 64 9785.0 65 7487.5 66 5602.0 67 4351.5 68 3080.0 69 1813.0 70 1321.5 71 992.5 72 745.0 73 592.0 74 441.5 75 344.5 76 239.5 77 141.0 78 89.0 79 57.0 80 35.5 81 16.5 82 13.0 83 10.5 84 7.5 85 3.5 86 0.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07088796503571047 2 0.0 3 0.0 4 0.0 5 0.0017174193701216408 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.776640727712042E-4 12-13 0.005389143540726528 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.92213575904014E-4 24-25 8.290990062656196E-4 26-27 3.553281455424084E-4 28-29 0.0026057397339776618 30-31 2.96106787952007E-4 32-33 0.0 34-35 0.0 36-37 5.92213575904014E-5 38-39 5.626028971088133E-4 40-41 1.480533939760035E-4 42-43 0.011785050160489878 44-45 1.480533939760035E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 2.96106787952007E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.96106787952007E-5 82-83 2.664961091568063E-4 84-85 3.8493882433760906E-4 86-87 0.0 88-89 0.0 90-91 0.008290990062656196 92-93 0.0028130144855440663 94-95 0.0 96-97 0.0 98-99 1.184427151808028E-4 100-101 0.010867119117838657 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1688580.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.29365314007201 #Duplication Level Percentage of deduplicated Percentage of total 1 79.21299459793879 46.17614831278642 2 12.049109930708878 14.047732698946808 3 3.670208678007749 6.418496148823978 4 1.6138522164744769 3.7630936530599826 5 0.8672278522158301 2.5276939805239618 6 0.555363695554812 1.9424507181156458 7 0.370381671342947 1.511363047509413 8 0.25602390871803454 1.193965514429965 9 0.18447515373018433 0.9678357562057944 >10 1.0946021309876552 11.719418279157152 >50 0.08300733674348851 3.335120656685162 >100 0.04062045806150567 4.3927886736962485 >500 0.0014215796771088354 0.5742234809812007 >1k 5.077070275388698E-4 0.6373815527477136 >5k 2.030828110155479E-4 0.7922875263306228 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7469 0.44232431984270804 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5916 0.3503535515048147 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4338 0.2569022492271613 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2623 0.1553376209596229 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.92213575904014E-5 7 0.0 0.0 0.0 0.0 5.92213575904014E-5 8 0.0 0.0 0.0 0.0 5.92213575904014E-5 9 0.0 0.0 0.0 0.0 5.92213575904014E-5 10-11 0.0 0.0 0.0 0.0 5.92213575904014E-5 12-13 0.0 0.0 0.0 2.96106787952007E-5 5.92213575904014E-5 14-15 0.0 0.0 0.0 5.92213575904014E-5 5.92213575904014E-5 16-17 0.0 0.0 0.0 1.480533939760035E-4 5.92213575904014E-5 18-19 0.0 0.0 0.0 1.776640727712042E-4 5.92213575904014E-5 20-21 0.0 0.0 0.0 2.368854303616056E-4 5.92213575904014E-5 22-23 0.0 0.0 0.0 3.849388243376091E-4 5.92213575904014E-5 24-25 0.0 0.0 0.0 4.1454950313280983E-4 5.92213575904014E-5 26-27 0.0 0.0 0.0 6.514349334944154E-4 5.92213575904014E-5 28-29 0.0 0.0 0.0 0.001184427151808028 5.92213575904014E-5 30-31 0.0 0.0 0.0 0.0024280756612064577 5.92213575904014E-5 32-33 0.0 0.0 0.0 0.003967830958556894 5.92213575904014E-5 34-35 0.0 0.0 0.0 0.005744471686268936 5.92213575904014E-5 36-37 0.0 0.0 0.0 0.008646318208198604 5.92213575904014E-5 38-39 0.0 0.0 0.0 0.013739354960973126 5.92213575904014E-5 40-41 0.0 0.0 0.0 0.024369588648450177 5.92213575904014E-5 42-43 0.0 0.0 0.0 0.03263096803231117 5.92213575904014E-5 44-45 0.0 0.0 0.0 0.042994705610631415 8.883203638560211E-5 46-47 0.0 0.0 0.0 0.06203437207594547 1.184427151808028E-4 48-49 0.0 0.0 0.0 0.0762178872188466 1.184427151808028E-4 50-51 0.0 0.0 0.0 0.09759679730898152 1.184427151808028E-4 52-53 0.0 0.0 0.0 0.11983441708417725 1.184427151808028E-4 54-55 0.0 0.0 0.0 0.14997808809769156 1.184427151808028E-4 56-57 0.0 0.0 0.0 0.218793305617738 1.184427151808028E-4 58-59 0.0 0.0 0.0 0.2748759312558481 1.184427151808028E-4 60-61 0.0 0.0 0.0 0.3221641852917836 1.184427151808028E-4 62-63 0.0 0.0 0.0 0.380349169124353 1.184427151808028E-4 64-65 0.0 0.0 0.0 0.43601724525933033 1.184427151808028E-4 66-67 0.0 0.0 0.0 0.5304456999372253 1.184427151808028E-4 68-69 0.0 0.0 0.0 0.6464307287780264 1.184427151808028E-4 70-71 0.0 0.0 0.0 0.7143280152554217 1.776640727712042E-4 72-73 0.0 0.0 0.0 0.7992514420400574 1.776640727712042E-4 74-75 0.0 0.0 0.0 0.9423302419784672 1.776640727712042E-4 76-77 0.0 0.0 0.0 1.104004548200263 1.776640727712042E-4 78-79 0.0 0.0 0.0 1.2668632815738667 1.776640727712042E-4 80-81 0.0 0.0 0.0 1.4249546956614434 1.776640727712042E-4 82-83 0.0 0.0 0.0 1.6407869333996614 1.776640727712042E-4 84-85 2.96106787952007E-5 0.0 0.0 1.939825178552393 1.776640727712042E-4 86-87 5.92213575904014E-5 0.0 0.0 2.188495659074489 1.776640727712042E-4 88-89 5.92213575904014E-5 0.0 0.0 2.531061602056165 1.776640727712042E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8870 0.0 30.117157 1 GGTATCA 6080 0.0 29.630358 1 GTACATG 6760 0.0 25.595049 1 TACATGG 6715 0.0 25.32309 2 ACATGGG 6565 0.0 24.816416 3 ATCAACG 10765 0.0 24.708914 3 TCAACGC 10905 0.0 24.435255 4 TATCAAC 11070 0.0 24.156858 2 CAACGCA 11025 0.0 24.083128 5 TATTCCG 200 2.0561856E-8 23.749239 5 AACGCAG 11310 0.0 23.476255 6 TAATCCG 125 2.2570023E-4 22.799269 4 TACCTGG 1450 0.0 20.964844 2 ACGCAGA 12720 0.0 20.762526 7 AGTACTT 6930 0.0 20.56272 12-13 GAGTACT 6775 0.0 20.472275 12-13 CGCAGAG 12835 0.0 20.465473 8 AGAGTAC 10320 0.0 20.435995 10-11 CATGGGG 3405 0.0 19.947966 4 GTACTTT 7015 0.0 19.703573 14-15 >>END_MODULE