##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937199_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 645319 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.829629996947247 33.0 31.0 34.0 30.0 34.0 2 31.931142582195783 33.0 31.0 34.0 30.0 34.0 3 31.927384750797668 33.0 31.0 34.0 30.0 34.0 4 35.603069179739016 37.0 35.0 37.0 33.0 37.0 5 35.638414489578025 37.0 35.0 37.0 33.0 37.0 6 35.71163564066764 37.0 35.0 37.0 35.0 37.0 7 35.669022607423614 37.0 35.0 37.0 33.0 37.0 8 35.66646108358812 37.0 35.0 37.0 33.0 37.0 9 37.25933685510577 39.0 37.0 39.0 34.0 39.0 10-11 37.16115130656311 39.0 37.0 39.0 34.0 39.0 12-13 37.053498347329 39.0 37.0 39.0 33.0 39.0 14-15 38.097905067106346 40.0 38.0 41.0 33.0 41.0 16-17 38.06158349591442 40.0 38.0 41.0 33.0 41.0 18-19 38.12899821638601 40.0 38.0 41.0 33.5 41.0 20-21 38.088723561525384 40.0 38.0 41.0 34.0 41.0 22-23 38.13720965909883 40.0 38.0 41.0 34.0 41.0 24-25 38.09316090181755 40.0 38.0 41.0 34.0 41.0 26-27 37.997230052113764 40.0 38.0 41.0 33.0 41.0 28-29 37.86769256755186 40.0 37.0 41.0 33.0 41.0 30-31 37.53179357805985 39.5 37.0 41.0 33.0 41.0 32-33 37.18742435911541 39.0 35.5 41.0 33.0 41.0 34-35 37.08069110006059 39.0 35.0 41.0 33.0 41.0 36-37 36.86245407310183 39.0 35.0 41.0 31.5 41.0 38-39 36.66268388192506 39.0 35.0 41.0 31.0 41.0 40-41 36.398761697702994 39.0 35.0 41.0 30.5 41.0 42-43 36.0207718973097 39.0 35.0 40.0 30.0 41.0 44-45 35.57773752206273 39.0 35.0 40.0 28.5 41.0 46-47 34.931446307950026 38.5 35.0 40.0 25.0 41.0 48-49 34.421520209384816 38.0 35.0 40.0 22.0 41.0 50-51 33.41408512689073 37.5 33.5 39.5 18.0 40.5 52-53 33.50052067272155 38.0 33.5 39.5 17.0 40.5 54-55 33.80345766977263 38.0 34.0 40.0 14.0 41.0 56-57 33.57965905234465 38.0 34.0 40.0 8.5 41.0 58-59 33.356426821463494 38.0 33.5 40.0 7.5 41.0 60-61 33.05619081415548 38.0 33.0 40.0 2.0 41.0 62-63 32.6989698118295 37.5 33.0 40.0 2.0 41.0 64-65 32.31050069810435 37.0 32.0 39.5 2.0 41.0 66-67 31.87806805626365 36.5 31.5 39.0 2.0 40.5 68-69 31.37506256595575 36.0 31.0 39.0 2.0 40.0 70-71 30.933873789552145 35.5 31.0 38.5 2.0 40.0 72-73 30.4462816064613 35.0 30.0 38.0 2.0 40.0 74-75 29.874733271451795 35.0 29.0 37.0 2.0 39.0 76-77 29.359692648132167 34.5 29.0 36.5 2.0 39.0 78-79 28.79270639792103 34.0 28.5 36.0 2.0 39.0 80-81 28.179820367911063 34.0 26.5 36.0 2.0 37.5 82-83 27.604921751877754 34.0 26.0 35.0 2.0 37.0 84-85 27.023514726825027 33.0 25.5 35.0 2.0 36.5 86-87 26.43201656854982 33.0 24.5 35.0 2.0 36.0 88-89 25.83221399029007 32.5 22.0 35.0 2.0 35.5 90-91 25.245226779313796 32.0 19.5 35.0 2.0 35.0 92-93 24.589547960001177 32.0 13.0 34.0 2.0 35.0 94-95 23.822294865020247 31.0 2.0 34.0 2.0 35.0 96-97 22.951987311701654 31.0 2.0 34.0 2.0 35.0 98-99 21.81027290378867 30.5 2.0 34.0 2.0 35.0 100-101 18.750061597442503 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.04441711599257303 1101 2 -0.012432306807969695 1101 3 0.03098190660685418 1101 4 -0.11881990138250842 1101 5 -0.1146088097968132 1101 6 -0.11523885875689643 1101 7 -0.16510419802497012 1101 8 -0.2402739856318732 1101 9 -0.2713450331225644 1101 10-11 -0.03504791164893106 1101 12-13 -0.061553450792779074 1101 14-15 0.18706384125307807 1101 16-17 0.12228504950385144 1101 18-19 0.07705864864396261 1101 20-21 0.035950494188348614 1101 22-23 -0.15938396554856382 1101 24-25 0.1435478591214192 1101 26-27 0.1131265365477887 1101 28-29 0.10260049008793004 1101 30-31 0.31165106772628093 1101 32-33 0.26502373922891564 1101 34-35 0.2773618147251753 1101 36-37 0.1929229913636874 1101 38-39 0.20634163174442222 1101 40-41 0.4141645729504688 1101 42-43 0.32625097678896964 1101 44-45 0.6563678903897454 1101 46-47 1.0440004048904754 1101 48-49 0.849312370660229 1101 50-51 0.6534759948589866 1101 52-53 0.619532861084501 1101 54-55 0.8068428445171705 1101 56-57 1.2131930546670944 1101 58-59 1.0971710766548455 1101 60-61 0.9286697260190238 1101 62-63 0.9402835296424641 1101 64-65 0.8981183004613555 1101 66-67 1.289369095035294 1101 68-69 1.4043214575404015 1101 70-71 1.3550293115468008 1101 72-73 1.1396900062105892 1101 74-75 1.1325609176331675 1101 76-77 1.1311413793545206 1101 78-79 1.0086776408603804 1101 80-81 1.2359754787908876 1101 82-83 1.0846677834754637 1101 84-85 1.0528155264967936 1101 86-87 1.3257370860903919 1101 88-89 1.4245929011668643 1101 90-91 1.0948945843709943 1101 92-93 1.5959503164529387 1101 94-95 1.613819357961063 1101 96-97 1.9019645781554715 1101 98-99 2.3519997839973854 1101 100-101 2.1102757746099527 1102 1 0.166119691071831 1102 2 -0.014153225605852526 1102 3 0.13615728750133727 1102 4 -0.043112763223959405 1102 5 0.09049946190902602 1102 6 -0.01971125428915599 1102 7 -0.16393477057930994 1102 8 -0.01810934885205029 1102 9 0.20166179075236101 1102 10-11 -0.08782496960867547 1102 12-13 0.1563919449188873 1102 14-15 0.19640264963309306 1102 16-17 0.15808547607908707 1102 18-19 0.07851046623985525 1102 20-21 -0.13239057995734527 1102 22-23 -0.03802929016435286 1102 24-25 0.09679733919131905 1102 26-27 0.1312759176150209 1102 28-29 -0.07699300098521888 1102 30-31 0.14008077915677575 1102 32-33 0.23413025679358412 1102 34-35 0.18112325152625885 1102 36-37 0.43202439268328163 1102 38-39 0.07902354083472574 1102 40-41 0.24031855199379493 1102 42-43 0.5573657202125943 1102 44-45 0.6629093751639346 1102 46-47 0.5964385946699409 1102 48-49 0.3919399943969495 1102 50-51 0.5280416123631895 1102 52-53 0.5466562689023888 1102 54-55 0.7373416451895949 1102 56-57 0.6262268551069567 1102 58-59 0.6753001256312672 1102 60-61 0.8311022679954903 1102 62-63 0.712627234999907 1102 64-65 0.575844694305907 1102 66-67 0.653901556613615 1102 68-69 0.7462427736778317 1102 70-71 0.4300819690042239 1102 72-73 0.5116709232809065 1102 74-75 0.40667205310735355 1102 76-77 0.4359931743592078 1102 78-79 0.6177632947762994 1102 80-81 0.6889060241028773 1102 82-83 0.3768186661938522 1102 84-85 0.3074251304196878 1102 86-87 0.9299556627111478 1102 88-89 0.9613169096452125 1102 90-91 0.9790315668495175 1102 92-93 1.2014479543423882 1102 94-95 1.3422530560760286 1102 96-97 1.2981646034044765 1102 98-99 1.2299806612008624 1102 100-101 0.6946041179432214 1103 1 0.15569770851362463 1103 2 0.27192395830414995 1103 3 0.11800784148811161 1103 4 0.1492754471209068 1103 5 0.183117885796797 1103 6 0.1587257885441744 1103 7 0.1714375969912041 1103 8 0.1679735053422604 1103 9 0.1446663610015122 1103 10-11 0.15786277917504776 1103 12-13 0.09942940281701595 1103 14-15 0.35122976266453776 1103 16-17 0.4507537984687744 1103 18-19 0.2080027489829135 1103 20-21 0.21892776114428614 1103 22-23 0.28575641845107924 1103 24-25 0.45867488373140475 1103 26-27 0.3640478928018567 1103 28-29 0.058312457230464076 1103 30-31 0.17271893563394514 1103 32-33 0.36688522112749666 1103 34-35 0.6572366118501449 1103 36-37 0.5524374161922765 1103 38-39 0.6740450312122448 1103 40-41 0.8146525771018176 1103 42-43 0.5266407176609746 1103 44-45 0.6648384058855328 1103 46-47 0.7613157196089873 1103 48-49 1.092102164528164 1103 50-51 1.2368838537131666 1103 52-53 1.1364021267596982 1103 54-55 1.3811729428908635 1103 56-57 1.226765532116218 1103 58-59 1.226059376700107 1103 60-61 1.2936659169368454 1103 62-63 1.3291742586676705 1103 64-65 1.3954718164098168 1103 66-67 1.5151803191307742 1103 68-69 1.668156154414742 1103 70-71 1.9151929364681308 1103 72-73 1.779286795540738 1103 74-75 1.6590246540667657 1103 76-77 1.5153408648524085 1103 78-79 1.4742733481903763 1103 80-81 1.4327747456805788 1103 82-83 1.748171255812693 1103 84-85 1.6980033860134256 1103 86-87 1.72945894580576 1103 88-89 1.6602293491149283 1103 90-91 1.6180295142065972 1103 92-93 1.6997196516852924 1103 94-95 1.6784997814868383 1103 96-97 1.761270331525683 1103 98-99 1.765836136994416 1103 100-101 1.645660491909812 1104 1 0.09103605437828577 1104 2 0.03733749213873594 1104 3 0.009737164796380426 1104 4 0.1131852215569964 1104 5 0.04326826173664955 1104 6 0.027898720875001004 1104 7 0.12482105563781687 1104 8 0.18752237752271128 1104 9 0.11308741363308883 1104 10-11 0.07590789195700154 1104 12-13 0.039279026877167666 1104 14-15 0.182056830333714 1104 16-17 0.028949287190577877 1104 18-19 -0.007786724701297487 1104 20-21 0.2730630994901446 1104 22-23 -0.024018017639143352 1104 24-25 -0.07140030423851584 1104 26-27 0.20013811836576423 1104 28-29 0.1575605775312141 1104 30-31 0.09301968751363887 1104 32-33 0.26763710082674663 1104 34-35 0.31513134869224757 1104 36-37 0.29228704025242536 1104 38-39 0.220661572565632 1104 40-41 0.3808179906356486 1104 42-43 0.3507008680369097 1104 44-45 0.20919930814118004 1104 46-47 0.22672925344357253 1104 48-49 0.14774126227252538 1104 50-51 0.33838761311166365 1104 52-53 0.1604622771356432 1104 54-55 0.2405714391314575 1104 56-57 0.08466026895832357 1104 58-59 0.22906689549710535 1104 60-61 0.37411704475638885 1104 62-63 0.338948694757903 1104 64-65 0.19246429761282258 1104 66-67 0.4505186649954318 1104 68-69 0.34108848524180857 1104 70-71 0.28812526729520016 1104 72-73 0.4567304045632987 1104 74-75 0.12970134579608938 1104 76-77 0.27097996259676904 1104 78-79 0.6441981602204514 1104 80-81 0.5553311366580225 1104 82-83 0.721855466339008 1104 84-85 0.9010109048104162 1104 86-87 0.8490078179862124 1104 88-89 0.7624849882126732 1104 90-91 0.7014881608231427 1104 92-93 0.7952083734898032 1104 94-95 1.1642140672011259 1104 96-97 1.3357064217512473 1104 98-99 1.6410241069192324 1104 100-101 1.7035552287519167 1105 1 0.23843711854376437 1105 2 0.06743661839141524 1105 3 0.33012061256195935 1105 4 0.25520017210268264 1105 5 0.0909010920033424 1105 6 0.18614817954338037 1105 7 0.356060514632631 1105 8 0.3182602206834346 1105 9 0.3411300237763939 1105 10-11 0.26652082080589423 1105 12-13 0.16316290444373038 1105 14-15 0.5110776457586681 1105 16-17 0.25467314821796805 1105 18-19 0.21320388496324227 1105 20-21 0.3195251301093833 1105 22-23 0.49445254371358516 1105 24-25 0.31471331555626847 1105 26-27 0.3398245268616833 1105 28-29 0.38566085717388177 1105 30-31 0.6384327861067263 1105 32-33 0.6016392552721896 1105 34-35 0.6487287285383943 1105 36-37 0.8018438555903344 1105 38-39 0.6298919567750687 1105 40-41 0.7136177904245571 1105 42-43 1.0269029506675125 1105 44-45 1.1708099193290593 1105 46-47 1.2693850971048235 1105 48-49 1.1017949928362682 1105 50-51 1.0379131998716176 1105 52-53 1.0901207213648831 1105 54-55 1.3047358298477576 1105 56-57 1.2564947030899631 1105 58-59 1.2021722348222639 1105 60-61 1.3744706784789074 1105 62-63 1.3820234583673638 1105 64-65 1.3634402178765797 1105 66-67 1.4346617288780088 1105 68-69 1.5277385401910486 1105 70-71 1.531938853358767 1105 72-73 1.8741944699365654 1105 74-75 1.813911872111877 1105 76-77 1.448622694607696 1105 78-79 1.5086585151598975 1105 80-81 1.5049954566475776 1105 82-83 1.6066198374694416 1105 84-85 1.709427387406194 1105 86-87 1.8595874508600012 1105 88-89 1.8200358842661188 1105 90-91 2.2171949386214713 1105 92-93 2.4487844265022183 1105 94-95 2.3544918600914535 1105 96-97 2.2381274526207484 1105 98-99 2.5466441628477625 1105 100-101 2.4251899958433043 1106 1 0.034143823801844775 1106 2 -0.5616713937122562 1106 3 -0.09550320886504338 1106 4 -0.1316085811315375 1106 5 -0.02448065601227256 1106 6 -0.2151520879652793 1106 7 -0.17989528065746185 1106 8 -0.07131790500177715 1106 9 -0.0023489044348039556 1106 10-11 -0.4708909232628713 1106 12-13 -0.35107754637414246 1106 14-15 -0.07888529771367558 1106 16-17 -0.14636399601744188 1106 18-19 -0.07288127926953081 1106 20-21 -0.1455573151828986 1106 22-23 -0.01034178159449084 1106 24-25 -0.17058576789766988 1106 26-27 -0.12507263579235683 1106 28-29 0.12798094663579462 1106 30-31 0.12484356539015096 1106 32-33 0.11909529175861877 1106 34-35 -0.19679050138749687 1106 36-37 -0.09655438148372752 1106 38-39 -0.10587636318282989 1106 40-41 -0.10422416019597591 1106 42-43 -0.3071824766885385 1106 44-45 -0.3396273038433293 1106 46-47 -0.359190090370511 1106 48-49 -0.06777480061196428 1106 50-51 -0.45994344179833035 1106 52-53 -0.5446641753592303 1106 54-55 -0.6569336873210361 1106 56-57 -0.9128448574941714 1106 58-59 -1.1124004091280923 1106 60-61 -0.9937571862757295 1106 62-63 -0.6066995166797824 1106 64-65 -0.810258418267793 1106 66-67 -0.6883341488573826 1106 68-69 -0.5683157070571205 1106 70-71 -0.506884985609787 1106 72-73 -0.6045363988087686 1106 74-75 -0.7898760066759998 1106 76-77 -0.713475494227108 1106 78-79 -0.4358257632245248 1106 80-81 -0.4701985967664477 1106 82-83 -0.40905134614674665 1106 84-85 -0.7033351876935683 1106 86-87 -0.5806572537841213 1106 88-89 -0.11317575455333184 1106 90-91 0.034485426715139766 1106 92-93 -0.08230358823268702 1106 94-95 0.007925618807416157 1106 96-97 0.2452131431000737 1106 98-99 0.2345021201340849 1106 100-101 0.19736361334977204 1107 1 0.22104170434134573 1107 2 0.22789770770655338 1107 3 0.14048382914511492 1107 4 0.15429087586618095 1107 5 0.079767023090902 1107 6 0.08806213519113726 1107 7 0.15084565470774436 1107 8 0.08131828347255521 1107 9 0.42842706333538416 1107 10-11 0.24207982467898148 1107 12-13 0.2662021873795837 1107 14-15 0.26312293490148875 1107 16-17 0.26221018510009486 1107 18-19 0.3232782933351288 1107 20-21 0.42445494615884627 1107 22-23 0.5108922348707452 1107 24-25 0.39579188142796085 1107 26-27 0.382726453365116 1107 28-29 0.357881104281617 1107 30-31 0.32608283671612526 1107 32-33 0.3072368226747173 1107 34-35 0.298786657484456 1107 36-37 0.012707310581433262 1107 38-39 0.13754192373257013 1107 40-41 0.20565935705748473 1107 42-43 0.1505509267096059 1107 44-45 0.3209305872058863 1107 46-47 0.46360286811609086 1107 48-49 0.3815552046429005 1107 50-51 0.3156302138331242 1107 52-53 0.49336787929131987 1107 54-55 0.5605255875081809 1107 56-57 0.8284583479708871 1107 58-59 0.8121441696072367 1107 60-61 0.7231109167195413 1107 62-63 0.5519403501970785 1107 64-65 0.48842348749504083 1107 66-67 0.8390611918327586 1107 68-69 0.9544071866195303 1107 70-71 0.8674844311006709 1107 72-73 0.7482837097916857 1107 74-75 0.8045188085280621 1107 76-77 0.9433777199960467 1107 78-79 0.8567780202752111 1107 80-81 0.7990998793239363 1107 82-83 1.0191847722627791 1107 84-85 0.9947818205390462 1107 86-87 0.7900863090114605 1107 88-89 1.098257946620251 1107 90-91 1.3750398145238627 1107 92-93 1.5371919451356781 1107 94-95 1.5091736482346327 1107 96-97 1.7815417470587356 1107 98-99 1.9178223163155508 1107 100-101 1.687466958944448 1108 1 0.335674192046703 1108 2 0.22415748329309082 1108 3 0.163159457975528 1108 4 0.2667132309635889 1108 5 0.3258671549524692 1108 6 0.27617220228297157 1108 7 0.22686094733262507 1108 8 0.22674957032538146 1108 9 0.49530119773466197 1108 10-11 0.27515924846879614 1108 12-13 0.34798227711179663 1108 14-15 0.5533904729049866 1108 16-17 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-0.25235666609807694 2316 70-71 -0.1056861844109882 2316 72-73 -0.1882859825583516 2316 74-75 -0.2144764062764004 2316 76-77 -0.3274198831714976 2316 78-79 -0.5587611611599215 2316 80-81 -0.26137408794193817 2316 82-83 -0.3315954686908711 2316 84-85 0.14905408969570644 2316 86-87 0.0624163392894701 2316 88-89 -0.15304421942180113 2316 90-91 0.27918239641211073 2316 92-93 0.5745895186604209 2316 94-95 0.7322013430831369 2316 96-97 0.7762404491273784 2316 98-99 1.1180435786755432 2316 100-101 1.5096763006624627 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2364.0 3 277.0 4 226.0 5 276.0 6 326.0 7 398.0 8 556.0 9 678.0 10 888.0 11 1543.0 12 2658.0 13 4047.0 14 4674.0 15 6785.0 16 9574.0 17 13409.0 18 10440.0 19 5480.0 20 5076.0 21 5100.0 22 5417.0 23 6141.0 24 6836.0 25 7648.0 26 8929.0 27 10534.0 28 12139.0 29 14795.0 30 17616.0 31 21667.0 32 26913.0 33 34530.0 34 45989.0 35 63663.0 36 83807.0 37 114065.0 38 82099.0 39 7756.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.06564945299895 23.50161504431657 15.798832940064603 19.633902562619884 2 14.038328868779407 23.372561328054594 41.4547816304841 21.1343281726819 3 16.89002627485895 29.89911137836574 32.907279736350205 20.303582610425106 4 10.385419175400298 20.107833464111188 41.73357054623709 27.77317681425142 5 9.761319864943186 41.39758631780615 35.942484318213246 12.898609499037416 6 24.266251205763435 42.5306353227718 19.371278831531743 13.831834639933021 7 23.47465314668804 38.028667822326604 22.860320478169832 15.636358552815524 8 24.315436616917896 35.58580109604702 21.466894906239514 18.631867380795565 9 26.36577173909666 17.135258240614444 23.593370845515473 32.90559917477342 10-11 21.869348513756222 29.764571900888082 29.209444468612695 19.156635116743004 12-13 23.695250789774374 29.265733152192063 29.16506459177578 17.87395146625778 14-15 21.25578050170949 29.741254102855265 29.37706501967351 19.625900375761738 16-17 17.14362268078067 32.96331963871048 30.347310132726523 19.545747547782323 18-19 17.000510978267116 30.63532148919879 35.26775105884326 17.09641647369083 20-21 17.96664952961112 29.60553190365765 35.49793501069383 16.929883556037396 22-23 18.2237467461062 29.434381620392518 34.89750622821678 17.444365405284508 24-25 17.612372181230647 30.47544272064974 34.981823660383185 16.930361437736426 26-27 17.26507248819043 30.317450826145603 35.68807674297425 16.729399942689717 28-29 16.555229792439274 30.41950954313341 36.05817136865814 16.967089295769178 30-31 17.46371177278874 30.01613327937449 35.61236941714353 16.907785530693236 32-33 16.970582387334492 30.484074909438863 35.371968609614704 17.173374093611944 34-35 17.426298861930313 30.691455196030233 34.88125722798131 17.000988714058145 36-37 17.8513931936632 30.056234094123184 34.50634604198491 17.5860266702287 38-39 16.693159554484623 31.507951360766352 34.4592616521467 17.339627432602324 40-41 16.87130379683928 31.030479698837095 35.04272376006361 17.055492744260015 42-43 16.884177892989914 30.75058652313126 34.414171920976834 17.951063662901994 44-45 17.501623008514194 30.99255124513486 33.55317721389233 17.952648532458618 46-47 17.9173099803977 31.437305258449026 32.77816782178372 17.867216939369555 48-49 17.589247158164202 31.497000500175528 32.67497600089472 18.238776340765547 50-51 17.577827041352922 31.60075376376994 32.18223311061157 18.639186084265567 52-53 17.749463573493927 32.48320802472301 31.74704907156652 18.020279330216542 54-55 18.19779656475358 31.701584811286683 31.5691612266851 18.531457397274636 56-57 18.19475073769219 32.540456592638606 30.865507066314645 18.39928560335456 58-59 18.205083410007646 31.683767950524683 31.511318681745305 18.599829957722367 60-61 18.394887742306185 31.91830015878747 31.711138805095175 17.97567329381117 62-63 18.143479901663124 31.505228223242725 32.099411562814 18.251880312280154 64-65 18.20808283622128 31.662136172598238 31.707014074424333 18.42276691675615 66-67 18.209121876336994 31.94706612696458 30.875219645445856 18.96859235125257 68-69 18.097605253983154 31.361226095052185 31.65830784533424 18.882860805630422 70-71 18.003054734411265 31.905668186268915 31.87942262879541 18.211854450524406 72-73 18.177293195599624 31.121704551719642 32.01842834168997 18.68257391099077 74-75 17.46157842381264 32.16105883659367 31.719126261014342 18.658236478579354 76-77 17.421416079299945 31.89277049674295 32.15665839331256 18.529155030644546 78-79 17.32526596756745 31.595836468873312 31.74525514170311 19.33364242185613 80-81 17.302695440814457 31.62254509873964 31.607557484876487 19.467201975569413 82-83 17.355071563462875 31.922538142331774 31.629093777954477 19.093296516250867 84-85 17.224764106812465 31.294192365236334 31.508714829535002 19.9723286984162 86-87 16.894292555818165 31.605084740498725 31.79818499734458 19.702437706338532 88-89 17.62080808692607 30.91169717201413 31.764464706279743 19.703030034780056 90-91 17.206821819074104 30.546765863571384 32.450619618281905 19.795792699072614 92-93 17.564237862385102 30.262169841576764 32.46585331193172 19.707738984106424 94-95 17.288433300279777 29.901483838053622 32.570124917592594 20.239957944074007 96-97 17.74210081632146 29.729570219797967 32.04279164544065 20.48553731843992 98-99 17.57863371517367 29.92674598570642 31.76551260337141 20.729107695748503 100-101 17.66103521584985 29.09535471307243 32.91789801271231 20.325712058365415 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1862.0 1 1167.0 2 558.5 3 674.5 4 612.5 5 630.0 6 1142.0 7 2286.0 8 3474.0 9 3761.5 10 3068.0 11 2390.0 12 2248.0 13 2259.5 14 2267.0 15 2199.5 16 2165.0 17 2167.0 18 2228.0 19 2468.0 20 2762.0 21 3084.0 22 3458.5 23 3892.0 24 4382.0 25 5471.0 26 7927.0 27 10961.0 28 11919.0 29 12738.0 30 14771.5 31 16602.5 32 19691.5 33 23895.0 34 28720.5 35 32098.5 36 36208.5 37 39953.5 38 37184.5 39 32572.5 40 29049.5 41 25796.0 42 23028.5 43 21464.0 44 20815.0 45 19627.0 46 17554.0 47 15551.0 48 15921.0 49 14330.0 50 10446.5 51 7691.0 52 5732.0 53 4675.0 54 3892.5 55 3368.5 56 3060.0 57 2722.0 58 2496.0 59 2295.0 60 2376.5 61 2423.5 62 1822.0 63 946.5 64 373.0 65 205.0 66 140.0 67 106.0 68 81.5 69 54.0 70 34.5 71 30.0 72 25.5 73 28.5 74 31.0 75 27.0 76 24.0 77 19.0 78 15.5 79 17.0 80 14.0 81 6.0 82 3.0 83 2.0 84 1.0 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 1.5 92 2.0 93 1.0 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30883950418320244 2 0.26823942887161234 3 0.2693241637081816 4 0.268704315230142 5 0.2693241637081816 6 0.2380218155671846 7 0.2372470049696352 8 0.24034624735983287 9 0.2505737472474854 10-11 0.26080124713513786 12-13 0.2514260389047897 14-15 0.24174090643542187 16-17 0.2346126489379671 18-19 0.2256248460063937 20-21 0.23089355806972986 22-23 0.2285691262770816 24-25 0.23050615277095515 26-27 0.2255473649466388 28-29 0.23732448602939013 30-31 0.2508061904267502 32-33 0.2409660958378724 34-35 0.24073365265860758 36-37 0.23128096336850457 38-39 0.22244812255644109 40-41 0.2192713991064884 42-43 0.2297313421734057 44-45 0.22515995964786406 46-47 0.23507753529649678 48-49 0.23941647464277357 50-51 0.2504187851279755 52-53 0.2646753001228849 54-55 0.2404237284195878 56-57 0.21989124758452797 58-59 0.21098092571270954 60-61 0.21330535750535784 62-63 0.218496588508939 64-65 0.20912138027859092 66-67 0.21439009234192702 68-69 0.21516490293947643 70-71 0.21702444837359505 72-73 0.2199687286442829 74-75 0.22353285739301026 76-77 0.23011874747218042 78-79 0.2309710391294848 80-81 0.22531492176737394 82-83 0.2192713991064884 84-85 0.20617710000790304 86-87 0.2096637476968755 88-89 0.21934888016624335 90-91 0.21919391804673347 92-93 0.22353285739301026 94-95 0.21919391804673347 96-97 0.22546988388688383 98-99 0.22678706190271788 100-101 0.2132278764456029 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 645319.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.27398535800676 #Duplication Level Percentage of deduplicated Percentage of total 1 77.19537030289405 26.457929914673013 2 11.380448484985797 7.801066494839069 3 3.768615757375938 3.874964438647694 4 1.8146713970201929 2.4878408356425505 5 1.0180139924796352 1.7445698336246513 6 0.6704073348641268 1.378651870742005 7 0.4466730778302596 1.0716486570559094 8 0.35031203325107113 0.9605271598704633 9 0.27895202355776577 0.86047178139047 >10 2.3589995435815534 17.113720839892924 >50 0.4073723900427593 9.82783020563008 >100 0.2911740501505257 18.58206836346749 >500 0.014016142165630897 3.185444303150958 >1k 0.004069202564215422 2.3385648948653004 >5k 9.04267236492316E-4 2.314700406507388 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8734 1.3534391517993425 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6203 0.9612300273198217 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4602 0.7131356739844945 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2417 0.374543442855394 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1420 0.22004620970403785 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1278 0.1980415887336341 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1179 0.18270033890215537 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1112 0.172317876894993 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1037 0.16069571793175158 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1034 0.16023083157322193 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1012 0.15682166494400443 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 957 0.14829874837096071 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 878 0.13605674092967973 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 870 0.13481704397360067 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 858 0.13295749853948202 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 824 0.1276887864761459 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 814 0.12613916528104704 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 783 0.12133533957624058 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 760 0.11777121082751321 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 745 0.11544677903486493 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 710 0.11002310485201892 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 683 0.10583912762525201 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 681 0.10552920338623224 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 677 0.1049093549081927 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 669 0.10366965795211361 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 668 0.10351469583260373 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 7.748105975494291E-5 0.0 20-21 0.0 0.0 0.0 1.5496211950988582E-4 0.0 22-23 0.0 0.0 0.0 4.648863585296574E-4 0.0 24-25 0.0 0.0 0.0 0.0012396969560790865 0.0 26-27 0.0 0.0 0.0 0.0019370264938735726 0.0 28-29 0.0 0.0 0.0 0.004261458286521859 0.0 30-31 0.0 0.0 0.0 0.00875535975230855 0.0 32-33 0.0 0.0 0.0 0.013714147576624893 0.0 34-35 0.0 0.0 0.0 0.018053086922901695 0.0 36-37 0.0 0.0 0.0 0.024174090643542187 0.0 38-39 0.0 0.0 0.0 0.03765579504090225 0.0 40-41 0.0 0.0 0.0 0.05214475321507657 0.0 42-43 0.0 0.0 0.0 0.06694363562827066 0.0 44-45 0.0 0.0 0.0 0.07329708252817599 0.0 46-47 0.0 0.0 0.0 0.08391198771460318 0.0 48-49 0.0 0.0 0.0 0.09925323754608187 0.0 50-51 0.0 0.0 0.0 0.12040556685918127 0.0 52-53 0.0 0.0 0.0 0.1715430662974436 0.0 54-55 0.0 0.0 0.0 0.2067969484859426 0.0 56-57 0.0 0.0 0.0 0.30209865198452235 0.0 58-59 0.0 0.0 0.0 0.4506298435347479 0.0 60-61 0.0 0.0 0.0 0.5789384784889333 0.0 62-63 0.0 0.0 0.0 0.643170277025781 0.0 64-65 0.0 0.0 0.0 0.7535807871765747 0.0 66-67 0.0 0.0 0.0 1.007021333634993 0.0 68-69 0.0 0.0 0.0 1.3765285076063156 0.0 70-71 0.0 0.0 0.0 1.7165153978110053 0.0 72-73 0.0 0.0 0.0 2.0861000528420828 0.0 74-75 0.0 0.0 0.0 2.5314611843134944 0.0 76-77 0.0 0.0 0.0 2.852930101236753 0.0 78-79 0.0 0.0 0.0 3.2619526156830965 0.0 80-81 0.0 0.0 0.0 3.8150124202138787 0.0 82-83 0.0 0.0 0.0 4.273312888664366 0.0 84-85 0.0 0.0 0.0 4.805297844941804 0.0 86-87 0.0 0.0 0.0 5.510840375070314 0.0 88-89 0.0 0.0 0.0 6.423489777923786 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCG 20 0.0020675114 71.30336 1 CATTCCG 175 0.0 40.719448 9 GTATCAA 7305 0.0 32.34111 1 TAGGCAT 225 0.0 31.673145 5 GGTATCA 4750 0.0 31.52359 1 GTGGTAC 110 2.715091E-6 30.24991 1 GTAAGCG 55 2.73357E-6 30.227581 94-95 GACGTGG 95 3.4854616E-5 30.003805 7 CTAAGTC 65 0.005840784 29.232206 3 ACGTGGA 100 4.9454895E-5 28.503614 8 TAGGACG 135 4.2155625E-7 28.151716 4 GTACATG 4545 0.0 28.029781 1 TGTAGGA 205 3.092282E-11 27.806242 2 TACTCCC 120 5.3683016E-6 27.713999 5 TTAACGT 70 0.00838051 27.148409 5 TAACGTA 70 0.008386866 27.14419 6 AGTATAC 70 0.008386866 27.14419 3 TATCAAC 8845 0.0 26.530415 2 TCAACGC 8860 0.0 26.487556 4 ATCAACG 8860 0.0 26.485498 3 >>END_MODULE