FastQCFastQC Report
Thu 26 May 2016
SRR937199_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937199_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645319
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99411.5404784300477747No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77701.2040556685918127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58330.9038940431011638No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26490.4104946545816875No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19140.29659749674192143No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16760.2597165122985686No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14100.218496588508939No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11320.17541711928519074No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT11170.17309268749254245No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA10470.16224533912685044No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC10400.16116060429028123No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA9250.14333996054664438No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8750.13559185457115008No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8480.13140787734438317No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA8380.1298582561492843No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8080.12520939256398775No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA7930.12288496077133945No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT7900.12242007441280979No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT7900.12242007441280979No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT7890.12226511229329991No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC7470.1157567032738847No Hit
GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG6960.10785363517888052No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA6840.10599408974476189No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG6800.10537424126672235No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGT250.00502110756.997636
TGGGCGA250.00502110756.997636
ATGGGCG902.2555469E-1047.4980245
TCCCGAT250.001616219937.9984276-77
GGTATCA51450.035.377291
CGTCACT550.002572463134.5627631
CATTCCG1800.034.3121039
ACATACG352.0539733E-433.9271616-17
GTATCAA78750.031.7428821
GGGTATG953.4839533E-530.0057287
GGCATTC2101.8189894E-1229.4103767
GTGTAGC2151.8189894E-1228.7353211
GTACATG42650.027.745431
CATGGGG16050.027.5222174
GACTGGT1056.8922556E-527.148047
ACGTGTC1405.789807E-727.1480398
GTAAGGT1955.347829E-1026.7937584
CTAGATA1257.386714E-626.5988944
TTAGGCA2355.456968E-1226.2755034
ATGGGGT5650.026.0608625
Produced by FastQC (version 0.11.5)