##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937198_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 634477 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.844388370264014 33.0 31.0 34.0 30.0 34.0 2 31.93547126215765 33.0 31.0 34.0 30.0 34.0 3 31.93466745051436 33.0 31.0 34.0 30.0 34.0 4 35.60121328275099 37.0 35.0 37.0 33.0 37.0 5 35.63959765287 37.0 35.0 37.0 33.0 37.0 6 35.72150448322004 37.0 35.0 37.0 35.0 37.0 7 35.68675460260971 37.0 35.0 37.0 33.0 37.0 8 35.68708715997585 37.0 35.0 37.0 33.0 37.0 9 37.297148675208085 39.0 37.0 39.0 34.0 39.0 10-11 37.19954545239622 39.0 37.0 39.0 34.0 39.0 12-13 37.10568625813071 39.0 37.0 39.0 33.5 39.0 14-15 38.21007065661955 40.0 38.0 41.0 33.5 41.0 16-17 38.21300850937071 40.0 38.0 41.0 33.5 41.0 18-19 38.31642833388759 40.0 38.0 41.0 34.0 41.0 20-21 38.34467758484547 40.0 38.0 41.0 34.0 41.0 22-23 38.20925187201428 40.0 38.0 41.0 34.0 41.0 24-25 38.05415956764391 40.0 38.0 41.0 33.0 41.0 26-27 37.88912285236502 40.0 37.0 41.0 33.0 41.0 28-29 37.91424511842037 40.0 37.0 41.0 33.0 41.0 30-31 37.66103184197378 39.5 37.0 41.0 33.0 41.0 32-33 37.34833492782244 39.0 36.0 41.0 33.0 41.0 34-35 37.16099165139162 39.0 35.0 41.0 33.0 41.0 36-37 37.0449630167839 39.0 35.0 41.0 32.5 41.0 38-39 36.88325660347026 39.0 35.0 41.0 32.0 41.0 40-41 36.66659547942636 39.0 35.0 41.0 31.0 41.0 42-43 36.304345153567425 39.0 35.0 41.0 30.5 41.0 44-45 35.86937824381341 39.0 35.0 41.0 29.5 41.0 46-47 35.25810391866057 39.0 35.0 41.0 25.5 41.0 48-49 34.75760035430756 39.0 35.0 41.0 23.0 41.0 50-51 33.831536840578934 38.0 34.0 40.0 19.5 40.5 52-53 33.902074464480194 38.0 34.5 40.0 19.0 40.5 54-55 34.22069751937423 39.0 35.0 40.0 16.0 41.0 56-57 34.06159009704055 39.0 35.0 40.0 11.0 41.0 58-59 33.870768365125926 38.5 34.0 40.0 8.0 41.0 60-61 33.62386185787665 38.0 34.0 40.0 4.5 41.0 62-63 33.32168620769547 38.0 34.0 40.0 2.0 41.0 64-65 32.98960403608011 38.0 33.5 40.0 2.0 41.0 66-67 32.62323772177715 37.0 33.0 40.0 2.0 41.0 68-69 32.1511678122296 36.5 32.5 39.0 2.0 41.0 70-71 31.747488876665347 36.0 32.0 39.0 2.0 40.5 72-73 31.350864570346914 35.5 31.5 38.5 2.0 40.0 74-75 30.87766696034687 35.0 31.0 37.5 2.0 40.0 76-77 30.433412085859693 35.0 31.0 37.0 2.0 39.0 78-79 29.942589723504554 35.0 30.5 36.5 2.0 39.0 80-81 29.43145772029561 35.0 29.5 36.0 2.0 38.5 82-83 28.928434758076335 34.0 29.0 36.0 2.0 37.0 84-85 28.46426820830385 34.0 29.0 35.0 2.0 37.0 86-87 28.042392395626635 34.0 28.5 35.0 2.0 36.5 88-89 27.619054749029516 34.0 27.0 35.0 2.0 36.0 90-91 27.201044324695772 34.0 26.0 35.0 2.0 36.0 92-93 26.78213552264306 34.0 25.5 35.0 2.0 35.0 94-95 26.27363403243932 33.5 25.0 35.0 2.0 35.0 96-97 25.70888621652164 33.0 22.0 35.0 2.0 35.0 98-99 25.057329895331115 33.0 10.5 35.0 2.0 35.0 100-101 23.24345326938565 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.048260710716864 1101 2 0.028229053475968158 1101 3 0.00706788426715832 1101 4 0.05124667529181437 1101 5 0.02040656098964888 1101 6 0.0438105586748776 1101 7 -0.165118111174678 1101 8 -0.0737496311392789 1101 9 -0.07428584279338679 1101 10-11 0.11045099073448483 1101 12-13 0.0454567030043691 1101 14-15 0.2004150189264351 1101 16-17 0.19455382014131573 1101 18-19 0.19756258590533804 1101 20-21 0.16160084515303907 1101 22-23 0.3046647067594819 1101 24-25 0.10973466482441552 1101 26-27 -0.1119887280991918 1101 28-29 -0.025782011805247862 1101 30-31 -0.007298828362500842 1101 32-33 -0.004556111336377455 1101 34-35 0.010004359928409201 1101 36-37 0.017389490172057265 1101 38-39 0.16418664130208782 1101 40-41 0.11200419890537461 1101 42-43 0.2959352820334331 1101 44-45 0.32011182443433484 1101 46-47 0.6173307250214748 1101 48-49 0.5808930895228528 1101 50-51 0.43837356184590703 1101 52-53 0.36854902225510244 1101 54-55 0.3619283405622298 1101 56-57 0.25709263340072397 1101 58-59 0.38454179427166224 1101 60-61 0.2204947026058406 1101 62-63 0.2688117087691353 1101 64-65 0.45103151375456463 1101 66-67 0.46927377158506545 1101 68-69 0.3586682471029832 1101 70-71 0.37306774233804774 1101 72-73 0.4253948286913882 1101 74-75 0.29791786812108256 1101 76-77 0.4680049990784596 1101 78-79 0.5301973984635744 1101 80-81 0.41940159370927077 1101 82-83 0.11814300562940616 1101 84-85 0.17344543836004434 1101 86-87 0.3616733519715112 1101 88-89 0.36800686079707035 1101 90-91 0.4383486915543564 1101 92-93 0.42035277372268354 1101 94-95 0.4135017370360252 1101 96-97 0.58283538493653 1101 98-99 0.7721329403048536 1101 100-101 1.2028718474252678 1102 1 0.1606891627679552 1102 2 0.15695776711034526 1102 3 0.16909453685984843 1102 4 0.004025552598989179 1102 5 0.006600660391299584 1102 6 -0.19866813663401217 1102 7 0.02500832423478272 1102 8 0.135738993443681 1102 9 0.042997293357672106 1102 10-11 0.16801711433834043 1102 12-13 0.21750268637111247 1102 14-15 0.39702344194995476 1102 16-17 0.47747739551773094 1102 18-19 0.33022552045506615 1102 20-21 0.4365030917534014 1102 22-23 0.35459904914665685 1102 24-25 0.3694675943686647 1102 26-27 0.19126776383654942 1102 28-29 0.3093032383743406 1102 30-31 0.20442131731990543 1102 32-33 0.39404633443587755 1102 34-35 0.5338295913008082 1102 36-37 0.5913679226410622 1102 38-39 0.5842644670402919 1102 40-41 0.418528911090462 1102 42-43 0.7864552442515063 1102 44-45 0.597656083110671 1102 46-47 0.6235582501169219 1102 48-49 1.0237720135338506 1102 50-51 0.975915684293696 1102 52-53 0.9511006425425776 1102 54-55 1.029697678142881 1102 56-57 1.0054490987383176 1102 58-59 0.8777456554000551 1102 60-61 1.0087389531142392 1102 62-63 0.9627519404452158 1102 64-65 1.2304407837298683 1102 66-67 1.3794987667157201 1102 68-69 1.4236588120935494 1102 70-71 1.2000813995403838 1102 72-73 1.0157740511460887 1102 74-75 1.3631754616805658 1102 76-77 1.4779405561838281 1102 78-79 1.370761446574793 1102 80-81 1.106833658499827 1102 82-83 0.9272123430006296 1102 84-85 1.4545479836135335 1102 86-87 1.3207520091633747 1102 88-89 1.449441149778533 1102 90-91 1.3522146997599869 1102 92-93 1.3977726285010306 1102 94-95 1.3952338414096381 1102 96-97 1.3640751095724823 1102 98-99 1.2219755505248209 1102 100-101 1.7230633959187038 1103 1 0.01603863725767596 1103 2 -0.12504150185644036 1103 3 0.095031467076101 1103 4 -0.00821390994197202 1103 5 -0.0772266191177593 1103 6 -0.025022097786646214 1103 7 0.026325039031362962 1103 8 0.06495702303129036 1103 9 0.01971751925121623 1103 10-11 0.060481487156735625 1103 12-13 0.1866774524725443 1103 14-15 0.021653917484925955 1103 16-17 -0.004238224011551495 1103 18-19 -0.09600638807600603 1103 20-21 -0.2888015588576138 1103 22-23 -0.30407363393761955 1103 24-25 -0.11913726507844302 1103 26-27 -0.019495310863504756 1103 28-29 -0.06067438174916617 1103 30-31 -0.06353869388071587 1103 32-33 -0.04361384627039655 1103 34-35 0.02761046516338439 1103 36-37 0.27865457102476654 1103 38-39 0.006259802962873096 1103 40-41 0.21426390941250872 1103 42-43 -0.13206340885185597 1103 44-45 0.1931574500572779 1103 46-47 0.6412890118663555 1103 48-49 0.6335477658888635 1103 50-51 0.5463011440002958 1103 52-53 0.5501738121273192 1103 54-55 0.38192209251474196 1103 56-57 0.44437041861664994 1103 58-59 0.6012002789713904 1103 60-61 0.39369206652485644 1103 62-63 -0.05786447873179412 1103 64-65 0.39955206536789234 1103 66-67 0.3834970053959239 1103 68-69 0.5736531476548947 1103 70-71 0.5849137428438418 1103 72-73 0.6823160289115613 1103 74-75 0.3190794099011818 1103 76-77 0.2580780417288224 1103 78-79 0.32919622323559494 1103 80-81 0.3085656942135806 1103 82-83 0.4451985388705033 1103 84-85 0.3065511642492744 1103 86-87 0.21854843565072102 1103 88-89 0.273572085949926 1103 90-91 0.16704954396654514 1103 92-93 0.028302670332376323 1103 94-95 -0.011097718491267727 1103 96-97 0.020183832594256046 1103 98-99 -0.07242456404646447 1103 100-101 0.14129882708936137 1104 1 0.17961489470262393 1104 2 0.19574653678292364 1104 3 0.15629845455035607 1104 4 0.11360568501924462 1104 5 0.12251276835901592 1104 6 0.10450693516072818 1104 7 0.10658489737043908 1104 8 0.06871956396149415 1104 9 0.3566953776615165 1104 10-11 0.3687615911503457 1104 12-13 0.29318187178217414 1104 14-15 0.35168385440579897 1104 16-17 0.2812026699480654 1104 18-19 0.3906466474104491 1104 20-21 0.31128915273608015 1104 22-23 0.4145339745234864 1104 24-25 0.2731201966309982 1104 26-27 0.329178686752428 1104 28-29 0.3078817955427624 1104 30-31 0.3889487520956081 1104 32-33 0.5298070282899161 1104 34-35 0.3435193513381378 1104 36-37 0.29558600521068 1104 38-39 0.5115066202010112 1104 40-41 0.49715524195115535 1104 42-43 0.8010268397565667 1104 44-45 0.6564276344343583 1104 46-47 0.8200662698622807 1104 48-49 0.6639529094247791 1104 50-51 0.6113233400588456 1104 52-53 0.7363368374113435 1104 54-55 0.8391933704588368 1104 56-57 0.900700013850205 1104 58-59 0.9616489766420031 1104 60-61 0.8911906890913031 1104 62-63 0.6791075138017817 1104 64-65 0.5384675485590193 1104 66-67 0.5622857856497134 1104 68-69 0.84914300073563 1104 70-71 0.887959599335165 1104 72-73 0.8781996499172635 1104 74-75 0.7852490133469665 1104 76-77 0.684059057237782 1104 78-79 0.7736233261000294 1104 80-81 0.7855442230097971 1104 82-83 0.8284958738770101 1104 84-85 0.9910853612631243 1104 86-87 1.0167046648975635 1104 88-89 0.6997699294022475 1104 90-91 0.8798363172525079 1104 92-93 0.9966604552935259 1104 94-95 0.8889163177137611 1104 96-97 1.0455872687410448 1104 98-99 1.3480875688910636 1104 100-101 1.4905248456045825 1105 1 0.2010844867498065 1105 2 0.3052811958576278 1105 3 0.32290534193842646 1105 4 0.2617326236001105 1105 5 0.37125230868353754 1105 6 0.17179734425478443 1105 7 0.28116212880183866 1105 8 0.29169151796948967 1105 9 0.2910291987394231 1105 10-11 0.24948562690379106 1105 12-13 0.31598019415823586 1105 14-15 0.4836112877960588 1105 16-17 0.33453529679901095 1105 18-19 0.37538357103291276 1105 20-21 0.36950262182272553 1105 22-23 0.41801577784694643 1105 24-25 0.3195197857843226 1105 26-27 0.37987423224514316 1105 28-29 0.36001000579192777 1105 30-31 0.25134160792836013 1105 32-33 0.31933879350408745 1105 34-35 0.4863374823933313 1105 36-37 0.38277068649881585 1105 38-39 0.6915261435699591 1105 40-41 0.7447127829033562 1105 42-43 0.43352795042839176 1105 44-45 0.6356061041119929 1105 46-47 0.7656280660122263 1105 48-49 1.1320328125823451 1105 50-51 1.0816999902726252 1105 52-53 0.9545481870508326 1105 54-55 0.978702922236522 1105 56-57 1.110571135160022 1105 58-59 1.1081853108951307 1105 60-61 1.2974999164305387 1105 62-63 1.231517977349327 1105 64-65 1.151223155923283 1105 66-67 1.1626028875502854 1105 68-69 1.3055190721773045 1105 70-71 1.3239767505536442 1105 72-73 1.1892356519878007 1105 74-75 1.0615953717699362 1105 76-77 0.9249433965612717 1105 78-79 0.7931833777819328 1105 80-81 1.0987699808140476 1105 82-83 1.1585284399976494 1105 84-85 1.3201509296214091 1105 86-87 1.0421798082659315 1105 88-89 1.103844566548819 1105 90-91 1.2283671735499162 1105 92-93 1.1038372294134469 1105 94-95 1.1529118016609878 1105 96-97 1.1647226158208959 1105 98-99 1.1445513528378797 1105 100-101 1.8824707426402085 1106 1 -0.08547573341489567 1106 2 -0.1895817675559357 1106 3 0.1140823924529677 1106 4 0.02659311183883517 1106 5 -0.15789602141249048 1106 6 -0.08015657554116018 1106 7 -0.14755912875478572 1106 8 -0.08129845127960778 1106 9 -0.2733777871269112 1106 10-11 -0.5075465965163914 1106 12-13 -0.4013233755472996 1106 14-15 -0.3703638849417814 1106 16-17 -0.2072238393230137 1106 18-19 -0.015816762905927817 1106 20-21 -0.06421303512643561 1106 22-23 -0.12613777528751058 1106 24-25 -0.008654835702714081 1106 26-27 0.03495022850302121 1106 28-29 -0.0793188442586299 1106 30-31 0.10022379380141189 1106 32-33 0.45495005680815126 1106 34-35 0.10815803641298061 1106 36-37 0.20981679606935444 1106 38-39 0.23641805394345283 1106 40-41 0.09990876221257139 1106 42-43 0.03227588953070182 1106 44-45 0.008354848694928307 1106 46-47 0.14804362689103812 1106 48-49 0.19013020022408966 1106 50-51 -0.1034766491111867 1106 52-53 -0.04295146684420814 1106 54-55 0.15912114287948498 1106 56-57 0.1305099270229917 1106 58-59 0.26418110729311906 1106 60-61 0.2841853636610736 1106 62-63 0.46802407928458223 1106 64-65 0.6355592846659377 1106 66-67 0.516290688294518 1106 68-69 0.2198633396304288 1106 70-71 0.298442595238523 1106 72-73 0.4973980654091221 1106 74-75 0.5906063086865352 1106 76-77 0.8393285673273603 1106 78-79 0.9319127320969187 1106 80-81 0.7614246268011193 1106 82-83 0.5897710197171584 1106 84-85 0.5492860448009402 1106 86-87 0.6955137429129259 1106 88-89 0.7241863084845725 1106 90-91 0.7211220431417615 1106 92-93 0.9047681468642672 1106 94-95 0.8011789695156004 1106 96-97 1.0845710008741989 1106 98-99 1.1427096549518474 1106 100-101 0.9412026560342639 1107 1 -0.06540340294725056 1107 2 0.14886767411458735 1107 3 0.18772056491983946 1107 4 0.15873178277691835 1107 5 0.05089459147767883 1107 6 0.09851511337942753 1107 7 0.20027575421919153 1107 8 -0.04420510159475555 1107 9 0.005745237237697154 1107 10-11 0.1601420404255407 1107 12-13 0.017373674921344673 1107 14-15 0.10099591950734066 1107 16-17 0.0012101267976234453 1107 18-19 0.011258149600905654 1107 20-21 -0.12870144514925386 1107 22-23 0.01650841860319474 1107 24-25 -0.017082961993210688 1107 26-27 0.18413181802135625 1107 28-29 0.053860567837325846 1107 30-31 0.11685479579112013 1107 32-33 0.12712702034675516 1107 34-35 0.08630768623173424 1107 36-37 0.10793783072039531 1107 38-39 0.3068495339649786 1107 40-41 0.04139887830005762 1107 42-43 -0.13986040376224906 1107 44-45 -0.14714314282063157 1107 46-47 -0.08534622765547795 1107 48-49 -0.04866254003277959 1107 50-51 -0.008670135197789364 1107 52-53 -0.01300460929852676 1107 54-55 0.007819486453371383 1107 56-57 0.09770572401381372 1107 58-59 0.004606914336783063 1107 60-61 0.023414005525147275 1107 62-63 0.1881053780628008 1107 64-65 0.31601837874142547 1107 66-67 0.21756251987380892 1107 68-69 0.20143658987132085 1107 70-71 0.20700232627263304 1107 72-73 0.31626321955977943 1107 74-75 0.30872187892509473 1107 76-77 0.2573175383909998 1107 78-79 0.4346355529017316 1107 80-81 0.5790290033366823 1107 82-83 0.4909923784787793 1107 84-85 0.44299070790531303 1107 86-87 0.6240498643480237 1107 88-89 0.38154887433908513 1107 90-91 0.33974359294925804 1107 92-93 0.6256659790358903 1107 94-95 0.5562293797636677 1107 96-97 0.6845207366218844 1107 98-99 0.7571849253568388 1107 100-101 0.8196069641603856 1108 1 0.09335093402490813 1108 2 0.09339271269903549 1108 3 0.07817499497823022 1108 4 0.27949807186003994 1108 5 0.18630886550581494 1108 6 0.14810670257497804 1108 7 0.04706024951853749 1108 8 -0.0966376342253028 1108 9 0.09568829747778551 1108 10-11 0.24047827917761566 1108 12-13 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-1.1832970046856488 2316 58-59 -1.3973184866289259 2316 60-61 -1.3240112286563246 2316 62-63 -1.259274195516383 2316 64-65 -1.279182609933649 2316 66-67 -1.311463405505279 2316 68-69 -1.4196897778961173 2316 70-71 -1.3775395092288996 2316 72-73 -1.2585490798206536 2316 74-75 -1.4067141388141842 2316 76-77 -1.583125998309736 2316 78-79 -1.3452516353347868 2316 80-81 -1.2522878812431593 2316 82-83 -1.3073900878499423 2316 84-85 -1.4921493635111496 2316 86-87 -2.00469252783007 2316 88-89 -2.107705846201622 2316 90-91 -2.461635521289743 2316 92-93 -2.5402651964922143 2316 94-95 -2.6809760820099306 2316 96-97 -2.586474937924656 2316 98-99 -2.721174136495506 2316 100-101 -3.4042010753866165 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2050.0 3 314.0 4 247.0 5 286.0 6 281.0 7 363.0 8 434.0 9 594.0 10 861.0 11 1437.0 12 2456.0 13 3752.0 14 4464.0 15 6577.0 16 9201.0 17 13207.0 18 9808.0 19 4925.0 20 4388.0 21 4113.0 22 4414.0 23 4847.0 24 5313.0 25 5991.0 26 6654.0 27 7709.0 28 9105.0 29 11043.0 30 13374.0 31 16283.0 32 20617.0 33 26585.0 34 36462.0 35 53839.0 36 76150.0 37 122567.0 38 125224.0 39 18539.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.234149743122664 23.34792845701238 15.824877491093496 19.593044308771464 2 14.094211145252455 23.47675859539977 41.31845655829951 21.110573701048267 3 16.93696626958098 29.94754169873857 32.754748353466844 20.360743678213606 4 10.37828747192441 19.947144390334884 41.75387684200377 27.920691295736937 5 9.780358040419431 41.633025956823985 35.605991154890575 12.980624847866014 6 24.32145136120395 42.31736364268159 19.45393893062251 13.907246065491949 7 23.482209131540298 38.012602852224454 22.776259890396247 15.728928125839007 8 24.448368281985637 35.425724966714945 21.501910238531067 18.623996512768354 9 26.44659685119703 17.037459352835548 23.553324183937598 32.962619612029826 10-11 21.993144517983794 29.681472822910575 29.16699575086483 19.158386908240796 12-13 23.752336592519587 29.233566064660927 29.19092103008291 17.82317631273657 14-15 21.271488056553398 29.704985142256085 29.38611418865189 19.637412612538625 16-17 17.177197097123184 33.09856040684498 30.24914516753137 19.47509732850047 18-19 16.894714316623 30.65148349418273 35.44237927609085 17.01142291310342 20-21 17.95427555319727 29.516619606208188 35.52219737598464 17.006907464609903 22-23 18.133414401111786 29.481218859313184 34.97721942784047 17.408147311734563 24-25 17.583792784634127 30.51438280616511 35.12573951065377 16.776084898546994 26-27 17.23229661048398 30.340467563557866 35.66236065195816 16.764875174 28-29 16.53642296248097 30.317828331006424 36.13323078089366 17.012517925618948 30-31 17.294181385107706 30.00903482197294 35.70263115497247 16.994152637946886 32-33 16.85417127333607 30.479909656632024 35.458508386770696 17.207410683261205 34-35 17.35319251102922 30.70372189779984 35.0314567731969 16.911628817974037 36-37 17.762309100183025 30.116652006866158 34.567493774190645 17.553545118760177 38-39 16.716424510074088 31.50472929811831 34.474407939567314 17.30443825224029 40-41 16.843340276056136 31.041392629133856 35.10383291987551 17.011434174934507 42-43 16.831447064313707 30.79862890496378 34.458119774200455 17.91180425652206 44-45 17.414385738550088 31.103507798045964 33.60917935545208 17.872927107951867 46-47 17.99832588404378 31.446451979721086 32.757474296002655 17.797747840232482 48-49 17.509455061508245 31.533508706395956 32.69946843166413 18.25756780043167 50-51 17.462499970383803 31.692095792209596 32.21223520157574 18.63316903583086 52-53 17.74674499377159 32.51025100851282 31.757628322247 17.985375675468593 54-55 18.188265192096136 31.84288937483169 31.471719629430446 18.49712580364173 56-57 18.146827887587488 32.65126304195928 30.89496559646609 18.30694347398714 58-59 18.164455722426887 31.79646259448688 31.490914890358113 18.54816679272812 60-61 18.3712702872766 31.959965861873226 31.686639786707765 17.98212406414241 62-63 18.121430663781062 31.659425021535437 32.11547890790254 18.10366540678096 64-65 18.234773038413014 31.69346013980714 31.701828988111497 18.369937833668352 66-67 18.186067001299477 31.982170460628012 30.855271572053162 18.976490966019348 68-69 18.072512680837626 31.52529259675923 31.628870862691695 18.77332385971145 70-71 18.079986861771467 31.99648173399351 31.872047550280964 18.05148385395406 72-73 18.16605622582548 31.196593005840178 32.08734449154449 18.55000627678985 74-75 17.381208053691275 32.32396367943151 31.779234109751282 18.515594157125935 76-77 17.409437540762777 32.03682033740922 32.202635032381245 18.351107089446764 78-79 17.26836378336851 31.685249360245276 31.818924738314458 19.22746211807176 80-81 17.132881213002683 31.790154894246008 31.75107009444309 19.32589379830822 82-83 17.260537215203463 32.127433471813646 31.64393663064915 18.968092682333733 84-85 17.288511062935495 31.386431423694717 31.520326653835042 19.804730859534743 86-87 16.89885429890546 31.75286267376572 31.771652424351515 19.576630602977307 88-89 17.491155616154135 31.09378244355266 31.79604349518365 19.619018445109553 90-91 17.170448318824146 30.621828994263172 32.478125685916275 19.729597000996403 92-93 17.483633814292347 30.506949115610166 32.49133926400323 19.518077806094254 94-95 17.222508186833487 30.114363037512593 32.52233418701665 20.140794588637277 96-97 17.59632358281728 29.85367910139042 32.11472935291647 20.435267962875827 98-99 17.427740370646422 30.101046627673416 31.860322262787555 20.61089073889261 100-101 17.250833659639504 29.78059594033018 32.69122067981154 20.277349720218773 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1851.0 1 1173.5 2 588.5 3 686.0 4 592.5 5 613.5 6 1135.0 7 2293.5 8 3557.5 9 3886.5 10 3121.0 11 2409.5 12 2303.0 13 2341.5 14 2308.0 15 2201.5 16 2189.5 17 2229.5 18 2336.5 19 2491.5 20 2812.0 21 3178.0 22 3520.5 23 3903.5 24 4307.5 25 5422.5 26 7976.5 27 10938.5 28 11799.5 29 12541.5 30 14372.0 31 16130.5 32 19193.0 33 23287.0 34 28007.0 35 31303.5 36 35242.5 37 39247.0 38 36799.0 39 32062.0 40 28496.5 41 25263.5 42 22672.5 43 21165.0 44 20304.5 45 19085.0 46 17099.5 47 15029.5 48 15673.0 49 14329.5 50 10264.0 51 7484.5 52 5577.0 53 4554.5 54 3806.0 55 3291.5 56 2987.0 57 2647.0 58 2439.0 59 2227.0 60 2352.5 61 2451.5 62 1795.5 63 881.5 64 299.0 65 176.0 66 137.5 67 97.0 68 65.0 69 52.5 70 37.5 71 30.0 72 21.5 73 26.5 74 41.0 75 41.0 76 29.5 77 20.0 78 21.0 79 22.5 80 15.5 81 9.0 82 6.5 83 3.0 84 0.5 85 0.5 86 1.5 87 1.0 88 0.5 89 1.0 90 2.0 91 2.0 92 1.0 93 0.5 94 0.5 95 1.0 96 1.0 97 2.5 98 3.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3268834016047863 2 0.2857471586834511 3 0.28117646502552496 4 0.28527432830504496 5 0.28543193843118037 6 0.21450738167025754 7 0.20284423233623913 8 0.20646926523735296 9 0.202371401957833 10-11 0.22175744747248521 12-13 0.21174920446288834 14-15 0.20576001966974375 16-17 0.20678448548962372 18-19 0.2011105209487499 20-21 0.19685504754309455 22-23 0.19890397918285457 24-25 0.18968378680393458 26-27 0.1915751083175592 28-29 0.20568121460667604 30-31 0.2160834829316114 32-33 0.21072473864300834 34-35 0.21844763482364213 36-37 0.19323001464198072 38-39 0.19598819184934993 40-41 0.18913215136246073 42-43 0.19866756399365146 44-45 0.20071649563341143 46-47 0.2059964348589468 48-49 0.212852475345836 50-51 0.21710794875149136 52-53 0.23491789300478977 54-55 0.21174920446288834 56-57 0.18668919440736229 58-59 0.18724082984883614 60-61 0.18400982226306076 62-63 0.19204793869596534 64-65 0.18534950833521152 66-67 0.18062120455115 68-69 0.17959673873127 70-71 0.19078705768688228 72-73 0.18779246529030996 74-75 0.1933876247681161 76-77 0.1954365564078761 78-79 0.1933088197050484 80-81 0.19520014121867302 82-83 0.18503428808294076 84-85 0.18062120455115 86-87 0.18172447543409767 88-89 0.18369460201079 90-91 0.18913215136246073 92-93 0.18267013619091 94-95 0.18014837417274385 96-97 0.18014837417274385 98-99 0.18992020199313767 100-101 0.17731139190230694 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 634477.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.434217650140155 #Duplication Level Percentage of deduplicated Percentage of total 1 77.22814435430033 26.592907314124204 2 11.361597894199479 7.824554694844781 3 3.799909111262001 3.925406921638392 4 1.8000326483857798 2.4793086396749664 5 1.0147195135702989 1.747053629206201 6 0.6466223889859976 1.3359561647878462 7 0.45710898872793226 1.1018133285385159 8 0.3448397435073219 0.9499429425879703 9 0.2863613955401255 0.8874567558563904 >10 2.3485883855307863 17.211077072030854 >50 0.40329538098980017 9.761299634022656 >100 0.29088654328395985 18.589837059703477 >500 0.014223159056471223 3.379367146899337 >1k 0.002752869494800882 1.8607847712099292 >5k 9.176231649336272E-4 2.353233924874503 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8644 1.3623819303142588 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6251 0.9852208984722851 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4571 0.72043588656484 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2402 0.3785795229771922 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1353 0.21324650066117445 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1281 0.20189857157942687 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1160 0.18282774631704538 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1011 0.15934383752287318 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 968 0.15256660209905165 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 956 0.15067528058542706 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 916 0.14437087554001168 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 901 0.14200672364798095 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 853 0.1344414375934825 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 844 0.13302294645826404 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 828 0.1305011844400979 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 781 0.12309350851173485 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 779 0.1227782882594641 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 746 0.11757715409699643 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 731 0.11521300220496566 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 720 0.11347929081747644 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 706 0.11127274905158106 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 699 0.11016947816863337 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 674 0.10622922501524877 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 674 0.10622922501524877 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 664 0.10465312375389493 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 658 0.10370746299708264 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 7.880506306769198E-4 0.0 24-25 0.0 0.0 0.0 0.0017337113874892234 0.0 26-27 0.0 0.0 0.0 0.003467422774978447 0.0 28-29 0.0 0.0 0.0 0.006856040486889202 0.0 30-31 0.0 0.0 0.0 0.012135979712424564 0.0 32-33 0.0 0.0 0.0 0.01757352906409531 0.0 34-35 0.0 0.0 0.0 0.021040951839073757 0.0 36-37 0.0 0.0 0.0 0.026399696127676814 0.0 38-39 0.0 0.0 0.0 0.03443781256058139 0.0 40-41 0.0 0.0 0.0 0.046652597336073645 0.0 42-43 0.0 0.0 0.0 0.05744889097634745 0.0 44-45 0.0 0.0 0.0 0.06469895677857511 0.0 46-47 0.0 0.0 0.0 0.07502242004044277 0.0 48-49 0.0 0.0 0.0 0.09204431366306423 0.0 50-51 0.0 0.0 0.0 0.11237601993452875 0.0 52-53 0.0 0.0 0.0 0.162968870423987 0.0 54-55 0.0 0.0 0.0 0.19378165008345455 0.0 56-57 0.0 0.0 0.0 0.2771574068090727 0.0 58-59 0.0 0.0 0.0 0.3969411026719645 0.0 60-61 0.0 0.0 0.0 0.5117600795615916 0.0 62-63 0.0 0.0 0.0 0.5739372743220006 0.0 64-65 0.0 0.0 0.0 0.6748865601117141 0.0 66-67 0.0 0.0 0.0 0.9089375974227593 0.0 68-69 0.0 0.0 0.0 1.2403916926854717 0.0 70-71 0.0 0.0 0.0 1.5411118133517843 0.0 72-73 0.0 0.0 0.0 1.8914003186876749 0.0 74-75 0.0 0.0 0.0 2.3222275984787473 0.0 76-77 0.0 0.0 0.0 2.605689410333235 0.0 78-79 0.0 0.0 0.0 2.990967363671181 0.0 80-81 0.0 0.0 0.0 3.5066676963861574 0.0 82-83 0.0 0.0 0.0 3.928353588861377 0.0 84-85 0.0 0.0 0.0 4.42915976465656 0.0 86-87 0.0 0.0 0.0 5.1158670842284275 0.0 88-89 0.0 0.0 0.0 6.0419053803368765 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGACG 15 6.5929093E-4 95.08554 3 CATTCCG 175 0.0 48.85479 9 GTCTTGC 45 9.5643575E-4 42.273613 1 GGTATCA 4580 0.0 34.785736 1 ATGGGCG 55 0.002566399 34.579296 5 TTAGGCA 260 0.0 32.91683 4 TAGGACA 335 0.0 32.64389 4 CATGGGT 540 0.0 32.57818 4 GTATCAA 7095 0.0 32.44254 1 TAGGACG 140 1.6872946E-8 30.565626 4 ATGGGTA 315 0.0 30.188276 5 TAGGCAT 290 0.0 29.511642 5 GACAATG 215 1.8189894E-12 28.719547 7 GTACATG 4855 0.0 28.309387 1 GGCATTC 305 0.0 28.031435 7 GACCATA 85 6.405781E-4 27.939829 7 CTTTAGG 330 0.0 27.373112 2 CCTTTAG 315 0.0 27.175894 1 GCCAGAC 70 0.008390748 27.141546 8 ACATGGG 4850 0.0 26.859217 3 >>END_MODULE