##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937198_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 634477 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17433728882213 33.0 31.0 34.0 31.0 34.0 2 32.386077036677456 34.0 31.0 34.0 31.0 34.0 3 32.43208973690142 34.0 31.0 34.0 31.0 34.0 4 36.08742003256225 37.0 35.0 37.0 35.0 37.0 5 35.97824192208701 37.0 35.0 37.0 35.0 37.0 6 36.08996228389682 37.0 35.0 37.0 35.0 37.0 7 36.046784989842024 37.0 35.0 37.0 35.0 37.0 8 36.03982335057063 37.0 35.0 37.0 35.0 37.0 9 37.71653503594299 39.0 37.0 39.0 35.0 39.0 10-11 37.65598674183619 39.0 37.0 39.0 35.0 39.0 12-13 37.58783612329525 39.0 37.0 39.0 35.0 39.0 14-15 38.7112716457807 40.0 38.0 41.0 34.5 41.0 16-17 38.71304475969972 40.0 38.0 41.0 34.5 41.0 18-19 38.86786282245062 40.0 38.0 41.0 35.0 41.0 20-21 38.87070138082231 40.0 38.0 41.0 35.0 41.0 22-23 38.74914929934418 40.0 38.0 41.0 35.0 41.0 24-25 38.59832665329082 40.0 38.0 41.0 34.0 41.0 26-27 38.37107649292251 40.0 38.0 41.0 34.0 41.0 28-29 38.30763447689987 40.0 38.0 41.0 34.0 41.0 30-31 38.16945846736761 40.0 37.0 41.0 34.0 41.0 32-33 37.917037969855485 40.0 36.5 41.0 33.5 41.0 34-35 37.72670955763566 40.0 36.0 41.0 33.0 41.0 36-37 37.479933078740444 40.0 35.5 41.0 33.0 41.0 38-39 37.26663456673764 40.0 35.0 41.0 33.0 41.0 40-41 37.035894130126074 40.0 35.0 41.0 33.0 41.0 42-43 36.67319303930639 40.0 35.0 41.0 32.0 41.0 44-45 36.11575281688698 40.0 35.0 41.0 30.5 41.0 46-47 35.418464341497014 39.0 35.0 41.0 26.5 41.0 48-49 34.81523758938464 39.0 35.0 41.0 21.5 41.0 50-51 34.51472630213546 39.0 35.0 41.0 16.5 41.0 52-53 34.29909437221522 39.0 35.0 41.0 11.0 41.0 54-55 34.093604653911804 39.0 35.0 40.5 7.5 41.0 56-57 33.879016103026586 39.0 34.5 40.0 2.0 41.0 58-59 33.6681763089915 38.0 34.0 40.0 2.0 41.0 60-61 33.38332673997639 38.0 34.0 40.0 2.0 41.0 62-63 33.05454650050356 38.0 33.5 40.0 2.0 41.0 64-65 32.756084144894146 37.5 33.0 40.0 2.0 41.0 66-67 32.38188775952477 37.0 33.0 39.5 2.0 41.0 68-69 31.93224892312881 36.5 32.5 39.0 2.0 40.5 70-71 31.570067157674746 36.0 32.0 39.0 2.0 40.0 72-73 31.145006044348335 35.5 31.5 38.5 2.0 40.0 74-75 30.674292369936182 35.0 31.0 37.5 2.0 39.5 76-77 29.31388293035051 34.0 29.5 36.0 2.0 39.0 78-79 29.841236955791935 35.0 31.0 36.0 2.0 39.0 80-81 29.609414525664445 35.0 30.5 36.0 2.0 38.5 82-83 29.224911226096452 34.5 30.0 36.0 2.0 37.0 84-85 28.843112516292948 34.0 30.0 35.0 2.0 37.0 86-87 28.489188733397743 34.0 29.0 35.0 2.0 36.5 88-89 28.072581039186606 34.0 29.0 35.0 2.0 36.0 90-91 27.62267111337369 34.0 28.5 35.0 2.0 36.0 92-93 27.232911516099087 34.0 27.0 35.0 2.0 35.0 94-95 26.837414910233154 34.0 26.0 35.0 2.0 35.0 96-97 26.44747090911097 34.0 25.0 35.0 2.0 35.0 98-99 25.911334059390647 33.0 24.5 35.0 2.0 35.0 100-101 24.47500933839997 31.5 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.003539304660549192 1101 2 0.15374758657559795 1101 3 0.19704682942420249 1101 4 0.175835727925012 1101 5 0.04862037289677801 1101 6 0.1081696327202053 1101 7 0.0853631397002701 1101 8 0.12966057508720752 1101 9 0.16416110517429416 1101 10-11 0.1409781055015955 1101 12-13 0.10305040563602574 1101 14-15 0.15234496861233282 1101 16-17 0.15003810783530724 1101 18-19 0.30090159784355563 1101 20-21 0.13795880700495644 1101 22-23 0.2463487553155801 1101 24-25 0.13282092524340783 1101 26-27 0.081853798129643 1101 28-29 0.22677617077755485 1101 30-31 0.19385502052063686 1101 32-33 0.060154701418234424 1101 34-35 -0.023711017123751787 1101 36-37 0.19206455312686188 1101 38-39 0.07933013323536642 1101 40-41 0.08406218859316539 1101 42-43 0.12287544643871229 1101 44-45 0.19085341618364993 1101 46-47 0.21555574193545368 1101 48-49 0.36231494359962113 1101 50-51 0.46338282492123994 1101 52-53 0.6207431148133438 1101 54-55 0.56506335477129 1101 56-57 0.3605223508359856 1101 58-59 0.21381804280473204 1101 60-61 0.18707684179995 1101 62-63 0.4056891571942671 1101 64-65 0.5906319076566575 1101 66-67 0.5283123931789291 1101 68-69 0.4216564258187816 1101 70-71 0.509572318845354 1101 72-73 0.47242317973316617 1101 74-75 0.46140696568615525 1101 76-77 0.5057238644087079 1101 78-79 0.6345317056057453 1101 80-81 0.5809497118267473 1101 82-83 0.4310556691765264 1101 84-85 0.4262132761634412 1101 86-87 0.521110618707791 1101 88-89 0.7071391848693978 1101 90-91 0.7335960692521972 1101 92-93 0.7241089531523137 1101 94-95 0.545115128309746 1101 96-97 0.5630250946706141 1101 98-99 0.5511786977922775 1101 100-101 0.8124522592894863 1102 1 0.08138389193909745 1102 2 0.013102068571591019 1102 3 -0.0345831757907078 1102 4 0.017650098041265494 1102 5 -0.10518389411503648 1102 6 -0.0793451585681737 1102 7 0.09912924780599752 1102 8 -0.08568173459472206 1102 9 0.003721963603076972 1102 10-11 -0.014904795664328674 1102 12-13 0.07501554741248384 1102 14-15 0.06944568759984548 1102 16-17 -0.011197930759230701 1102 18-19 -0.11182404129717582 1102 20-21 -0.15528254284016896 1102 22-23 -0.07235455395376533 1102 24-25 -0.04150579625313355 1102 26-27 0.07202191093927013 1102 28-29 -0.0423540700508056 1102 30-31 -0.02393452745759106 1102 32-33 0.030821351330168056 1102 34-35 0.22680995037834606 1102 36-37 0.14202863516641173 1102 38-39 0.348948355683774 1102 40-41 0.31169723698292984 1102 42-43 0.47982457697275294 1102 44-45 0.6682627992534051 1102 46-47 0.6898372256508907 1102 48-49 1.121456221608824 1102 50-51 0.9658709028810151 1102 52-53 0.7069755406684166 1102 54-55 0.8116794221420776 1102 56-57 0.7686980325965749 1102 58-59 0.9196132957698069 1102 60-61 0.8546608301386556 1102 62-63 0.7933591392038721 1102 64-65 0.9108640863794051 1102 66-67 0.8003464387978809 1102 68-69 0.92246379455068 1102 70-71 0.8230785191440084 1102 72-73 0.7773467676756205 1102 74-75 0.5741150123564616 1102 76-77 0.5715375056940495 1102 78-79 0.9879979458644357 1102 80-81 1.1780988938815895 1102 82-83 0.98135861570589 1102 84-85 0.8647548184785698 1102 86-87 0.8791446182512246 1102 88-89 0.8503712148938831 1102 90-91 0.9063122419683722 1102 92-93 0.865384868013134 1102 94-95 1.1481818728859245 1102 96-97 1.143648873407603 1102 98-99 1.2201271497595947 1102 100-101 0.9259100802944751 1103 1 0.133468939744823 1103 2 -0.02249198141655029 1103 3 -0.0011501625872227805 1103 4 -0.01188834639700076 1103 5 -0.07494220427405196 1103 6 -0.008460587002794284 1103 7 -0.015230424810823706 1103 8 -0.15428343050628257 1103 9 -0.08164005815264375 1103 10-11 0.026601645764309012 1103 12-13 -0.03628105328306219 1103 14-15 0.13148839105472376 1103 16-17 -0.19616322805675424 1103 18-19 -0.17816744308115062 1103 20-21 -0.25033502974249444 1103 22-23 -0.2769252215666427 1103 24-25 -0.11222898475496379 1103 26-27 -0.2840660780995137 1103 28-29 -0.18965619410842294 1103 30-31 -0.23022376438289172 1103 32-33 -0.20308148965300887 1103 34-35 -0.3099682879171084 1103 36-37 -0.26399084622750735 1103 38-39 -0.33755595890846024 1103 40-41 -0.6623414650269766 1103 42-43 -0.5865754691977756 1103 44-45 -0.8742824788491532 1103 46-47 -0.9818230533814898 1103 48-49 -0.899217910716736 1103 50-51 -1.047662830454314 1103 52-53 -0.8218938587514586 1103 54-55 -0.8046499788595654 1103 56-57 -0.9295135010555313 1103 58-59 -0.9474113349194866 1103 60-61 -0.9979323071267245 1103 62-63 -0.9608555237834011 1103 64-65 -0.901282821371467 1103 66-67 -0.6528214786765929 1103 68-69 -0.5515439133609377 1103 70-71 -0.642091595705157 1103 72-73 -0.6309299390838703 1103 74-75 -0.9037690953661972 1103 76-77 -0.862524153323843 1103 78-79 -0.5180694160675401 1103 80-81 -0.5456699457207463 1103 82-83 -0.44220566284768736 1103 84-85 -0.574091688059049 1103 86-87 -0.38846377024804113 1103 88-89 -0.4049314478197914 1103 90-91 -0.5071917797830743 1103 92-93 -0.3080164243338892 1103 94-95 -0.16100349533465064 1103 96-97 -0.2309009105955937 1103 98-99 -0.24643663075088895 1103 100-101 -0.08761617170678093 1104 1 -0.05277264638824164 1104 2 0.1534089020263849 1104 3 0.047662311618630326 1104 4 0.07267036160175167 1104 5 -0.0012688875187976123 1104 6 0.0925141960121394 1104 7 -0.00882195448483003 1104 8 0.02642747185410599 1104 9 -0.05154811053492381 1104 10-11 0.03039246860016931 1104 12-13 0.02095942880820445 1104 14-15 -0.00406468779731739 1104 16-17 0.004915883765711726 1104 18-19 0.03620730683348228 1104 20-21 0.1658510191076914 1104 22-23 0.3113792677145142 1104 24-25 0.11045645551051564 1104 26-27 0.24285724860678215 1104 28-29 0.2827176214558449 1104 30-31 0.18161106090825996 1104 32-33 0.2842325044434091 1104 34-35 0.21594422253148338 1104 36-37 0.4362848499787333 1104 38-39 0.28037225533011423 1104 40-41 0.3083134391602087 1104 42-43 0.11313877880730416 1104 44-45 0.22949116534771719 1104 46-47 0.14770388460990347 1104 48-49 0.18966792884734218 1104 50-51 0.4579977405887803 1104 52-53 0.7757426119181119 1104 54-55 0.6702608283898215 1104 56-57 0.8287547664368233 1104 58-59 0.6331177252623164 1104 60-61 0.5186366996756036 1104 62-63 0.3189017546173929 1104 64-65 0.43295064471224265 1104 66-67 0.5495376512462897 1104 68-69 0.47906653586046843 1104 70-71 0.40571110972098623 1104 72-73 0.3810563515242329 1104 74-75 0.477922455885853 1104 76-77 0.4635986907045897 1104 78-79 0.36448106713405437 1104 80-81 0.4215052365927967 1104 82-83 0.2853987340459092 1104 84-85 0.2197583591822081 1104 86-87 0.4695977250896348 1104 88-89 0.5844974043328826 1104 90-91 0.7783989035287178 1104 92-93 0.7129457051469608 1104 94-95 0.37366069369741695 1104 96-97 0.13574990423753164 1104 98-99 0.2929799659093355 1104 100-101 0.3129058913220639 1105 1 0.0382188880507357 1105 2 0.09698677336778871 1105 3 0.043739296460508115 1105 4 0.1955167791246879 1105 5 -0.05458317300018223 1105 6 0.09813684475045648 1105 7 0.22203067464860737 1105 8 0.12710121149632414 1105 9 0.12645080528634622 1105 10-11 0.08528920668948814 1105 12-13 0.1240318120042545 1105 14-15 0.10688938948913318 1105 16-17 0.2614128044535562 1105 18-19 0.17226256734206657 1105 20-21 0.1494976502056815 1105 22-23 0.20495652767693429 1105 24-25 0.12549841648822735 1105 26-27 0.16400668185846 1105 28-29 0.1786578611979266 1105 30-31 0.02723073426884781 1105 32-33 0.08990161798463703 1105 34-35 0.442247173046475 1105 36-37 0.36590901493693906 1105 38-39 0.2446830070409547 1105 40-41 0.36568728110650994 1105 42-43 -0.045709709252463426 1105 44-45 -0.10953172875736783 1105 46-47 0.16491114616191282 1105 48-49 0.07551633946577141 1105 50-51 0.0999976427681446 1105 52-53 0.18053154351294864 1105 54-55 0.1380961799531164 1105 56-57 0.08413795840574778 1105 58-59 0.09760577687900707 1105 60-61 -0.1079900779088021 1105 62-63 0.08118232050977525 1105 64-65 0.2311405553286363 1105 66-67 0.2162933075208784 1105 68-69 0.31231495544579246 1105 70-71 0.3799058217003477 1105 72-73 0.619105930284114 1105 74-75 0.5248814568110731 1105 76-77 0.3552124051579959 1105 78-79 0.32876838265044483 1105 80-81 0.41899450689160034 1105 82-83 0.47446768536791595 1105 84-85 0.5511145608068482 1105 86-87 0.450040730302657 1105 88-89 0.5096340475361814 1105 90-91 0.564897210008926 1105 92-93 0.696639208633858 1105 94-95 0.473816799131761 1105 96-97 0.5623986914654182 1105 98-99 0.8946502532982166 1105 100-101 0.9903311298617687 1106 1 -0.12005176182949384 1106 2 3.626601306407906E-4 1106 3 -0.062078622966254215 1106 4 -0.09572319956363629 1106 5 -0.04937062260520264 1106 6 -0.10412059860369993 1106 7 -0.06366742953527904 1106 8 -0.014449422609907003 1106 9 -0.07354961423481399 1106 10-11 -0.12221120234364236 1106 12-13 -0.3095265245104528 1106 14-15 0.0805382167177342 1106 16-17 0.09003945998892249 1106 18-19 -0.05915684054577497 1106 20-21 -0.29484972170401846 1106 22-23 0.043982063929853155 1106 24-25 0.03944204123494188 1106 26-27 0.1518604955155496 1106 28-29 0.3024323350135063 1106 30-31 0.1420358457146378 1106 32-33 0.012531428319128679 1106 34-35 -0.05954942631501581 1106 36-37 0.0446460045370074 1106 38-39 0.0488285053691655 1106 40-41 0.14680211663324627 1106 42-43 0.14680645280017046 1106 44-45 0.09325116484787088 1106 46-47 0.07309579420464019 1106 48-49 -0.055150866984782 1106 50-51 0.3166561986924137 1106 52-53 0.3345576880715484 1106 54-55 0.3865072435651129 1106 56-57 0.39614842965387 1106 58-59 0.33787708121224114 1106 60-61 0.4600101066805067 1106 62-63 0.44523753788467246 1106 64-65 0.398611304215855 1106 66-67 0.5174465711857223 1106 68-69 0.588041041690957 1106 70-71 0.2174997419997169 1106 72-73 0.3570950395148351 1106 74-75 0.42098179788177603 1106 76-77 0.3126257635507237 1106 78-79 0.4534857302698754 1106 80-81 0.5488698671553962 1106 82-83 0.39722964486734114 1106 84-85 0.6101632126756051 1106 86-87 0.7527556614802116 1106 88-89 0.7081892166661383 1106 90-91 0.5110537669148734 1106 92-93 0.44936190095284445 1106 94-95 0.18718316276430969 1106 96-97 0.310279060811844 1106 98-99 0.3403642153818929 1106 100-101 0.35005269366883596 1107 1 0.010051311172553312 1107 2 0.05016598299399533 1107 3 0.008420640798014745 1107 4 0.09425064633992974 1107 5 -0.08252625824985671 1107 6 -0.13799161344103794 1107 7 -0.02160396726684155 1107 8 -0.0432960978694652 1107 9 -0.08338764237445417 1107 10-11 -0.08618362166013327 1107 12-13 -0.09928429669867711 1107 14-15 -0.019464703197336064 1107 16-17 0.026578234375428167 1107 18-19 0.02181031875228001 1107 20-21 -0.02928136023521688 1107 22-23 0.02511628408310429 1107 24-25 -0.00749246506998702 1107 26-27 -0.054019587743738384 1107 28-29 0.2002008942022755 1107 30-31 0.0083183349839544 1107 32-33 0.040886932150463906 1107 34-35 -0.014979008391740933 1107 36-37 -0.01830840724610283 1107 38-39 -0.11418931028408252 1107 40-41 -0.025291420760446215 1107 42-43 0.19892902249228683 1107 44-45 0.48913846446870224 1107 46-47 0.3848864112661161 1107 48-49 0.2572810885657688 1107 50-51 0.3047544162928233 1107 52-53 0.3740453681155955 1107 54-55 0.25318079288873463 1107 56-57 0.2086001989138353 1107 58-59 0.34357717256386877 1107 60-61 0.3289257336488376 1107 62-63 0.15553190703701603 1107 64-65 0.10478442180917114 1107 66-67 0.36204347691001004 1107 68-69 0.4431996973620542 1107 70-71 0.22038002965306802 1107 72-73 0.3795345868445743 1107 74-75 0.5150385193177058 1107 76-77 0.2964609051457465 1107 78-79 0.3204846415586786 1107 80-81 0.3929432738203076 1107 82-83 0.2888090269298864 1107 84-85 0.23821608816625428 1107 86-87 0.12254622014339489 1107 88-89 0.09453882226903687 1107 90-91 0.2432068288629594 1107 92-93 0.29958902863238634 1107 94-95 0.2747743579689761 1107 96-97 0.48398376562929 1107 98-99 0.42060556721914466 1107 100-101 0.21627991311714112 1108 1 0.010297123299558564 1108 2 -0.1027352045606591 1108 3 -0.08219538051107378 1108 4 0.05485220100089094 1108 5 -0.17641641649645123 1108 6 -0.17868762443408315 1108 7 -0.021704709941843703 1108 8 0.007734096725087625 1108 9 -0.03258714107889915 1108 10-11 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-1.0789963844190247 2316 78-79 -1.1252681021787083 2316 80-81 -1.2612022714000943 2316 82-83 -1.4051719760722285 2316 84-85 -1.6694291918398712 2316 86-87 -1.5470788160843298 2316 88-89 -1.496247852490768 2316 90-91 -1.5071677521258273 2316 92-93 -1.6404178614704712 2316 94-95 -1.710051010733551 2316 96-97 -1.9818104280919684 2316 98-99 -2.116890810008517 2316 100-101 -2.375810959683907 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 14.0 6 27.0 7 66.0 8 186.0 9 393.0 10 784.0 11 1500.0 12 2521.0 13 3658.0 14 4681.0 15 7015.0 16 10534.0 17 15318.0 18 12441.0 19 5743.0 20 3737.0 21 3369.0 22 3587.0 23 3816.0 24 4340.0 25 4913.0 26 5589.0 27 6681.0 28 8005.0 29 9771.0 30 12167.0 31 14873.0 32 18717.0 33 24344.0 34 33804.0 35 49447.0 36 71036.0 37 127784.0 38 145206.0 39 18408.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.175394610278 23.572228266868507 16.543040509642527 18.70933661321097 2 14.722834712684621 24.10899055442514 41.00889393941782 20.15928079347242 3 17.227417227102006 29.72054148534935 33.549521889682374 19.502519397866276 4 10.759255260631985 20.692633460314557 41.38463648012458 27.16347479892888 5 9.82148346061996 41.342381323938554 35.81727472631405 13.018860489127437 6 24.60483201124706 42.509342340226674 18.78807269609458 14.097752952431689 7 23.261836126447452 38.3451251976037 22.748184725372237 15.644853950576618 8 24.785926046176616 35.33477178841786 21.224252415769207 18.655049749636316 9 26.467626092041165 16.92543622542661 23.66106257594838 32.94587510658385 10-11 22.207345577538664 29.797928057281826 28.91436569016686 19.08036067501265 12-13 24.273494304434927 29.082530920920934 29.390518582500185 17.25345619214395 14-15 21.51133926052481 29.320999815596156 30.186279408079407 18.98138151579963 16-17 16.958770767104244 32.43710961941883 31.26984902526018 19.33427058821675 18-19 16.656159324924307 29.271746651178844 37.35005366624795 16.722040357648897 20-21 17.546026097084685 28.30937922099619 37.6899399032589 16.45465477866022 22-23 17.863728781502672 28.119532838432075 37.17220672372216 16.84453165634309 24-25 17.400137437113063 28.93224602504129 37.532783164584096 16.134833373261547 26-27 17.145378962507635 28.859073625312764 37.968201430344585 16.02734598183502 28-29 16.373700764193774 28.836592406607515 38.43618071760294 16.353526111595777 30-31 17.326930078538116 28.456945887033093 37.81429825570674 16.40182577872205 32-33 16.972010017699617 28.98371414566643 37.49607944811238 16.548196388521568 34-35 17.271863282672186 28.987733203882883 37.40584765089988 16.334555862545056 36-37 17.746942159311114 28.402042316842806 36.97400451238858 16.877011011457498 38-39 16.754731671643224 29.891035809949805 36.81471832201294 16.539514196394023 40-41 16.89030247771194 29.527492349992475 37.24109561189422 16.34110956040137 42-43 16.89526651329473 29.344086707949412 36.5797585298362 17.180888248919658 44-45 17.54799622366138 29.378763926824767 35.685690734258294 17.387549115255556 46-47 18.186947675014224 30.023625757907695 34.50881592240538 17.2806106446727 48-49 17.680940365056575 30.36540331643228 34.19067988280111 17.762976435710044 50-51 17.672916175776407 30.535567511168654 33.49832499706451 18.293191315990427 52-53 18.042383039219008 31.462286565832727 32.77573365717037 17.719596737777895 54-55 18.519583846222954 30.734289816652137 32.506930905296805 18.239195431828104 56-57 18.581682235920294 31.447002807036345 31.886656253890997 18.08465870315236 58-59 18.548662914494933 30.7744015937536 32.39510652080374 18.281828970947725 60-61 18.6719140331328 30.95620487425076 32.62931516824093 17.74256592437551 62-63 18.49160804883392 30.648786323223693 32.96218775463886 17.897417873303525 64-65 18.555046124603415 30.834057026495838 32.553425892506745 18.057470956394006 66-67 18.449526145155772 31.190413521687944 31.64582798115613 18.714232352000153 68-69 18.535266053773423 30.640432986538517 32.263817285732976 18.560483673955083 70-71 18.419107390811646 31.116651982656578 32.76139245394238 17.70284817258939 72-73 18.64811490408636 30.123235357625255 33.06881100496945 18.15983873331894 74-75 17.945173741522545 31.3844315869606 32.35483713357616 18.3155575379407 76-77 17.736182714267027 31.08355385616815 33.08512365302446 18.095139776540364 78-79 17.50221048201905 30.862111628947936 32.573048353210595 19.062629535822417 80-81 17.4574492061966 30.975118089387006 32.49755310279175 19.069879601624645 82-83 17.40779180250743 31.391411317554923 32.400592931630825 18.800203948306827 84-85 17.232724326846093 30.729373530283254 32.25338704694898 19.784515095921673 86-87 16.783981137220106 30.804899153160793 32.554844383641964 19.856275325977144 88-89 17.431364730321196 30.206926334603146 32.68526676301899 19.67644217205667 90-91 17.04291109471839 29.751445396483135 33.21527423127605 19.99036927752243 92-93 17.13790581626638 29.551651635558944 33.143040875576474 20.1674016725982 94-95 16.97461058478085 29.3633181344635 33.13815946046902 20.52391182028663 96-97 17.394247545616313 29.177022965371478 32.664935056747524 20.763794432264685 98-99 16.871090222186496 29.32784526224374 32.45779528328502 21.343269232284737 100-101 16.604495403950324 29.444273242502604 32.93038263502645 21.020848718520625 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1726.0 1 1169.0 2 739.5 3 883.0 4 716.0 5 667.0 6 1268.5 7 2494.5 8 3642.5 9 3891.5 10 3111.5 11 2384.0 12 2264.5 13 2210.5 14 2185.0 15 2133.0 16 2129.0 17 2266.5 18 2392.0 19 2635.5 20 3019.0 21 3378.5 22 3817.5 23 4300.5 24 4833.0 25 5893.0 26 8186.0 27 11180.5 28 12205.0 29 12605.0 30 14141.5 31 15723.5 32 18765.0 33 22668.5 34 27174.0 35 30900.0 36 35189.0 37 38591.5 38 36317.5 39 32148.0 40 28664.5 41 25354.5 42 22344.0 43 21158.0 44 20599.5 45 19546.0 46 17528.5 47 15280.5 48 16148.0 49 14559.0 50 10251.0 51 7545.0 52 5481.0 53 4404.0 54 3701.0 55 3191.5 56 2888.5 57 2585.0 58 2284.0 59 2142.0 60 2297.5 61 2234.0 62 1506.5 63 687.5 64 249.0 65 160.5 66 125.5 67 95.0 68 64.0 69 44.5 70 38.0 71 30.0 72 21.0 73 26.0 74 29.5 75 26.5 76 24.0 77 22.0 78 18.0 79 14.5 80 10.5 81 4.5 82 5.0 83 4.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04838630872356287 2 0.0 3 0.0 4 0.0 5 0.012924030343101483 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005673964540873822 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0011032708829476878 24-25 7.880506306769198E-4 26-27 0.002285346828963067 28-29 0.0027581772073692187 30-31 0.0012608810090830715 32-33 0.0 34-35 0.0 36-37 2.3641518920307594E-4 38-39 3.940253153384599E-4 40-41 7.880506306769197E-5 42-43 0.011269124018679952 44-45 0.0 46-47 0.0 48-49 0.0 50-51 7.880506306769197E-5 52-53 2.3641518920307594E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.940253153384599E-4 84-85 1.5761012613538393E-4 86-87 0.0 88-89 0.0 90-91 0.007722896180633813 92-93 0.0023641518920307593 94-95 0.0 96-97 0.0 98-99 0.0015761012613538395 100-101 0.01158434427095072 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 634477.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.753441834808946 #Duplication Level Percentage of deduplicated Percentage of total 1 76.21676992511789 24.96361540579365 2 11.826173366983472 7.746957630077196 3 3.995153166519705 3.9256505058226776 4 1.7746530162287375 2.3250397737606487 5 1.0773765660479055 1.764389534511814 6 0.6925049296205849 1.360915195958777 7 0.5063390245813995 1.1609042053224539 8 0.3796911462618002 0.9948953499422248 9 0.3051166687936872 0.8994258957748229 >10 2.4725750514188287 17.32582637962164 >50 0.4280986633550581 9.779703304468011 >100 0.30426447372898185 18.612268185019133 >500 0.01596300100644897 3.3686947422397875 >1k 0.0038698184258058113 1.9121802673357162 >5k 9.674546064514528E-4 2.219231834002102 >10k+ 4.837273032257264E-4 1.6403017903493804 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10353 1.63173763587963 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8077 1.273016988795496 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5930 0.9346280479828267 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2732 0.43059086460186896 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1977 0.31159521936965406 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1679 0.26462740178130967 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1325 0.20883341712938375 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1136 0.17904510328979617 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1129 0.17794183240684847 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1050 0.16549063244215315 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1041 0.16407214130693468 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 951 0.14988722995475012 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 840 0.1323925059537225 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 825 0.13002835406169175 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 816 0.1286098629264733 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 795 0.12530005027763025 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 782 0.12325111863787025 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 772 0.12167501737651641 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 765 0.12057174649356872 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 728 0.11474017182655952 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 725 0.11426734144815336 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 714 0.11253363006066414 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 699 0.11016947816863337 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 641 0.10102809085278111 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 635 0.10008243009596882 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 7.880506306769198E-4 0.0 24-25 0.0 0.0 0.0 0.002206541765895375 0.0 26-27 0.0 0.0 0.0 0.003940253153384599 0.0 28-29 0.0 0.0 0.0 0.007407675928363045 0.0 30-31 0.0 0.0 0.0 0.0127664202169661 0.0 32-33 0.0 0.0 0.0 0.018203969568636846 0.0 34-35 0.0 0.0 0.0 0.021671392343615294 0.0 36-37 0.0 0.0 0.0 0.027266551821421424 0.0 38-39 0.0 0.0 0.0 0.03514705812819062 0.0 40-41 0.0 0.0 0.0 0.04728303784061519 0.0 42-43 0.0 0.0 0.0 0.05831574667009206 0.0 44-45 0.0 0.0 0.0 0.06532939728311665 0.0 46-47 0.0 0.0 0.0 0.07510122510351044 0.0 48-49 0.0 0.0 0.0 0.09220192378919961 0.0 50-51 0.0 0.0 0.0 0.11206079968225799 0.0 52-53 0.0 0.0 0.0 0.16241723498251315 0.0 54-55 0.0 0.0 0.0 0.1945697007141315 0.0 56-57 0.0 0.0 0.0 0.2792063384488327 0.0 58-59 0.0 0.0 0.0 0.39930525456399524 0.0 60-61 0.0 0.0 0.0 0.5151486972735024 0.0 62-63 0.0 0.0 0.0 0.5778775274753852 0.0 64-65 0.0 0.0 0.0 0.6799300841480463 0.0 66-67 0.0 0.0 0.0 0.9157148328465807 0.0 68-69 0.0 0.0 0.0 1.2491390546859855 0.0 70-71 0.0 0.0 0.0 1.5525385474965996 0.0 72-73 0.0 0.0 0.0 1.904481959156912 0.0 74-75 0.0 0.0 0.0 2.338697856659895 0.0 76-77 0.0 0.0 0.0 2.6257058963524287 0.0 78-79 0.0 0.0 0.0 3.0158697636005716 0.0 80-81 0.0 0.0 0.0 3.53661362035188 0.0 82-83 0.0 0.0 0.0 3.959796809025386 0.0 84-85 0.0 0.0 0.0 4.466119339235307 0.0 86-87 0.0 0.0 0.0 5.161337605618486 0.0 88-89 0.0 0.0 0.0 6.099984711817765 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGGCCT 25 0.0016150037 38.004192 90-91 GGTATCA 5300 0.0 34.78565 1 GTATCAA 8080 0.0 31.814869 1 GACATAT 270 0.0 31.67765 1 TACAATC 135 1.1941665E-8 31.67016 7 GCGCCTT 30 0.003949734 31.668913 84-85 CATTCCG 245 0.0 31.023834 9 CGTCACT 65 0.0058322316 29.240908 1 CACCGCA 65 0.0058389762 29.233995 9 ATGACCG 65 0.005841226 29.23169 5 CATATAG 295 0.0 28.983967 3 CCTTTAG 310 0.0 27.590212 1 GACGTGG 70 0.0083842715 27.145853 7 GACCGCG 70 0.0083842715 27.145853 7 GGCATTC 285 0.0 26.66961 7 GTGGTAC 125 7.386785E-6 26.598743 6 CATGGGG 1685 0.0 25.935537 4 GTACATG 4200 0.0 25.907795 1 TATCAAC 9990 0.0 25.676485 2 ATCAACG 10030 0.0 25.526728 3 >>END_MODULE