##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937197_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 687219 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.771541531884306 33.0 31.0 34.0 30.0 34.0 2 31.85218103690381 33.0 31.0 34.0 30.0 34.0 3 31.860193038900263 33.0 31.0 34.0 30.0 34.0 4 35.54194514412436 37.0 35.0 37.0 33.0 37.0 5 35.555418287329076 37.0 35.0 37.0 33.0 37.0 6 35.65787761979806 37.0 35.0 37.0 33.0 37.0 7 35.613303764884265 37.0 35.0 37.0 33.0 37.0 8 35.61009954614177 37.0 35.0 37.0 33.0 37.0 9 37.21457934079238 39.0 37.0 39.0 34.0 39.0 10-11 37.14487957987192 39.0 37.0 39.0 34.0 39.0 12-13 37.05955088552558 39.0 37.0 39.0 33.0 39.0 14-15 38.15373410804998 40.0 38.0 41.0 33.0 41.0 16-17 38.12465094824212 40.0 38.0 41.0 33.0 41.0 18-19 38.21052968558786 40.0 38.0 41.0 33.5 41.0 20-21 38.22907617513485 40.0 38.0 41.0 34.0 41.0 22-23 38.10861675826774 40.0 38.0 41.0 33.5 41.0 24-25 37.96550808403144 40.0 38.0 41.0 33.0 41.0 26-27 37.80992958576524 40.0 37.0 41.0 33.0 41.0 28-29 37.63220894649304 40.0 37.0 41.0 33.0 41.0 30-31 37.497306535471225 39.0 36.5 41.0 33.0 41.0 32-33 37.23160666396011 39.0 35.5 41.0 33.0 41.0 34-35 37.00744231460422 39.0 35.0 41.0 32.0 41.0 36-37 36.86698054040997 39.0 35.0 41.0 31.5 41.0 38-39 36.76396752709107 39.0 35.0 41.0 31.0 41.0 40-41 36.57069798710455 39.0 35.0 41.0 31.0 41.0 42-43 36.22195035352631 39.0 35.0 41.0 30.0 41.0 44-45 35.80874000864353 39.0 35.0 41.0 29.5 41.0 46-47 35.23886926874839 39.0 35.0 41.0 25.5 41.0 48-49 34.75856604663142 39.0 35.0 41.0 23.0 41.0 50-51 33.787234491479424 38.0 34.0 39.5 20.5 40.5 52-53 33.89269214035119 38.0 34.5 39.5 20.0 40.5 54-55 34.25198444746144 39.0 35.0 40.0 18.5 41.0 56-57 34.07558071007932 39.0 34.5 40.0 14.0 41.0 58-59 33.883160244405346 38.0 34.0 40.0 10.0 41.0 60-61 33.636695143760576 38.0 34.0 40.0 7.5 41.0 62-63 33.342342106373664 38.0 34.0 40.0 4.0 41.0 64-65 33.02974524860343 38.0 33.0 40.0 2.0 41.0 66-67 32.65590590481346 37.0 33.0 40.0 2.0 41.0 68-69 32.16488339233927 36.5 32.5 39.0 2.0 41.0 70-71 31.771115175802766 36.0 32.0 39.0 2.0 40.5 72-73 31.36690341797884 35.5 31.5 38.5 2.0 40.0 74-75 30.8955944175001 35.0 31.0 37.5 2.0 40.0 76-77 30.45378110907877 35.0 31.0 37.0 2.0 39.0 78-79 29.96916412380915 35.0 30.5 36.5 2.0 39.0 80-81 29.488904555898483 35.0 30.0 36.0 2.0 38.5 82-83 29.02461515179295 34.0 29.0 36.0 2.0 37.0 84-85 28.545198110063893 34.0 29.0 35.0 2.0 37.0 86-87 28.117401439715724 34.0 29.0 35.0 2.0 36.5 88-89 27.71192298233896 34.0 27.5 35.0 2.0 36.0 90-91 27.28660223305817 34.0 26.5 35.0 2.0 36.0 92-93 26.869117413808407 34.0 26.0 35.0 2.0 35.0 94-95 26.375566595219283 33.5 25.0 35.0 2.0 35.0 96-97 25.81123702924395 33.0 23.5 35.0 2.0 35.0 98-99 25.202317601812524 33.0 19.0 35.0 2.0 35.0 100-101 23.478403536572767 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11301733009712223 1101 2 0.11027240301093144 1101 3 0.2693727811754556 1101 4 0.16568236236724943 1101 5 0.10206788466144445 1101 6 -0.040640258620250336 1101 7 0.1545546769073809 1101 8 0.04610080155399743 1101 9 0.16736838080636574 1101 10-11 0.22632529161517567 1101 12-13 0.10981821131772307 1101 14-15 0.30841867342297036 1101 16-17 0.3463496306339664 1101 18-19 0.3614393497927608 1101 20-21 0.41647448498483897 1101 22-23 0.30939249324155327 1101 24-25 0.2603457610992663 1101 26-27 0.33997241363917396 1101 28-29 0.38542214559557664 1101 30-31 0.19026170098764084 1101 32-33 -0.07816801531802042 1101 34-35 0.09097583213515748 1101 36-37 0.03757773493641281 1101 38-39 0.47191090035134664 1101 40-41 0.49709518095826866 1101 42-43 0.6514136161158959 1101 44-45 0.7206373846949248 1101 46-47 0.856533514832698 1101 48-49 0.81024734427141 1101 50-51 0.6863281339502194 1101 52-53 0.7737388783459593 1101 54-55 0.7790061955485896 1101 56-57 1.0022248501702222 1101 58-59 0.8743033158549949 1101 60-61 0.700803011662579 1101 62-63 0.8571585864231039 1101 64-65 1.1563414146180264 1101 66-67 0.9787340407768639 1101 68-69 0.6946334077082668 1101 70-71 0.5779453508447752 1101 72-73 0.745387091165032 1101 74-75 0.9777275416621336 1101 76-77 0.9192666662745523 1101 78-79 1.0038409399564436 1101 80-81 1.0062819119253845 1101 82-83 1.0373202070665357 1101 84-85 0.7819297785084309 1101 86-87 0.9621726165797533 1101 88-89 0.9628917737963967 1101 90-91 0.8174474587721754 1101 92-93 0.6180739300995661 1101 94-95 0.7732064664700289 1101 96-97 0.8328891069647888 1101 98-99 1.1960553509614549 1101 100-101 1.430282310456164 1102 1 0.12009351808617197 1102 2 0.055154353702011605 1102 3 0.12237455118967233 1102 4 -0.07666643682802032 1102 5 -0.08795426469680478 1102 6 -0.06172567389653949 1102 7 0.02320608082849418 1102 8 0.05485028167277051 1102 9 0.2541880618169188 1102 10-11 0.08547761923222907 1102 12-13 0.20590290117834087 1102 14-15 0.32091613446815614 1102 16-17 0.3828399729334322 1102 18-19 0.31721027319853334 1102 20-21 0.38564528652079133 1102 22-23 0.42384857657730635 1102 24-25 0.40266651088889205 1102 26-27 0.4501514462248437 1102 28-29 0.25558434853785883 1102 30-31 0.38858357275349675 1102 32-33 0.33742745421935894 1102 34-35 0.22480244811945482 1102 36-37 0.3570633668701859 1102 38-39 0.40900382126169177 1102 40-41 0.5696570589143448 1102 42-43 0.38971072702712206 1102 44-45 0.645043269266786 1102 46-47 0.5615030957358869 1102 48-49 0.7950716485977907 1102 50-51 0.7521968433874022 1102 52-53 0.9207951415847901 1102 54-55 1.1721060417604647 1102 56-57 1.1685549239691753 1102 58-59 1.0976965201610582 1102 60-61 1.0929956046850506 1102 62-63 1.1114322519000837 1102 64-65 0.8477553948286811 1102 66-67 1.212000635869181 1102 68-69 1.1147670776344683 1102 70-71 1.0484905732572152 1102 72-73 1.0379162770552135 1102 74-75 1.1918538026763557 1102 76-77 1.3614036440961783 1102 78-79 1.278085782251079 1102 80-81 1.1691183838904848 1102 82-83 1.0290417635183857 1102 84-85 0.9456057982010506 1102 86-87 0.8763336975458138 1102 88-89 1.1499010301002563 1102 90-91 1.1091999662923193 1102 92-93 0.8117444366331448 1102 94-95 0.7057505475618271 1102 96-97 1.0034894609676321 1102 98-99 1.2967372416848413 1102 100-101 1.4632867886888583 1103 1 -0.06097010493930455 1103 2 -0.16007782556286543 1103 3 -0.13966313864093394 1103 4 -0.026232946612516628 1103 5 -0.28220932843415625 1103 6 -0.08256178601330788 1103 7 -0.12932531148491222 1103 8 -0.1367695426446005 1103 9 -0.008693743026739753 1103 10-11 0.0010642124754980387 1103 12-13 0.037405730773812707 1103 14-15 0.018603869076869728 1103 16-17 0.05556343475771541 1103 18-19 0.1052850050062446 1103 20-21 0.14015834839079844 1103 22-23 0.13897405055836742 1103 24-25 0.04383474320153624 1103 26-27 0.12708786130839655 1103 28-29 -0.15031111839302724 1103 30-31 0.04409318575629584 1103 32-33 0.18806863864801215 1103 34-35 0.19050813208126272 1103 36-37 0.04911477595156555 1103 38-39 -0.020040023177244848 1103 40-41 0.18166616697249083 1103 42-43 0.19437908319410724 1103 44-45 0.28680695517216037 1103 46-47 0.26489765757732187 1103 48-49 0.6096950361094642 1103 50-51 0.21773865852049568 1103 52-53 0.3751357600943521 1103 54-55 0.4369199744218193 1103 56-57 0.5770398994841202 1103 58-59 0.3785629289566259 1103 60-61 0.3638488658652861 1103 62-63 0.569971532782688 1103 64-65 0.561148813399214 1103 66-67 0.6986519954088308 1103 68-69 0.6424618624137182 1103 70-71 0.8553911658526268 1103 72-73 0.9778049986877981 1103 74-75 0.8011943259133432 1103 76-77 0.6241530200608096 1103 78-79 0.7627926285383069 1103 80-81 0.5588689602895656 1103 82-83 0.6186570528611952 1103 84-85 0.8126157637123796 1103 86-87 0.9248979398430279 1103 88-89 1.0396411265161056 1103 90-91 1.2454730937098617 1103 92-93 1.293445433184278 1103 94-95 1.0755805812883175 1103 96-97 1.4652508603312135 1103 98-99 1.2544914765507933 1103 100-101 1.4244784580549137 1104 1 -0.10586556955515647 1104 2 -0.02546782785475088 1104 3 -0.034916755634345265 1104 4 -0.01801854266611258 1104 5 0.003688671805946342 1104 6 0.048808651591059515 1104 7 0.10011048483570306 1104 8 0.15413604264077208 1104 9 0.3329261461624782 1104 10-11 0.032626731801904896 1104 12-13 0.03651808039165161 1104 14-15 0.12995942762359647 1104 16-17 0.12516213675092303 1104 18-19 0.14618877133592179 1104 20-21 0.2721344982436449 1104 22-23 0.36901555912746886 1104 24-25 0.39356626534004846 1104 26-27 0.23896636961921303 1104 28-29 0.26988220021888765 1104 30-31 0.30077511952448077 1104 32-33 0.22257968516598226 1104 34-35 0.052618557243967246 1104 36-37 0.1917436398682142 1104 38-39 0.30309290974330594 1104 40-41 0.24243474851445512 1104 42-43 0.2156754165432062 1104 44-45 0.33233541637201114 1104 46-47 0.19992728836568574 1104 48-49 0.15806379663666092 1104 50-51 0.15804053240865557 1104 52-53 0.1993436958342869 1104 54-55 0.15789035090926262 1104 56-57 0.26139105979596877 1104 58-59 0.08310176911771094 1104 60-61 0.18964474993232727 1104 62-63 0.3087694940971417 1104 64-65 0.2979659319551047 1104 66-67 0.34248137008822965 1104 68-69 0.26367797981639995 1104 70-71 0.3150221724990878 1104 72-73 0.2979375641793034 1104 74-75 0.5625728033019044 1104 76-77 0.47528695704490787 1104 78-79 0.5530379329041644 1104 80-81 0.6407728976010745 1104 82-83 0.6623028949103578 1104 84-85 0.7489295772257627 1104 86-87 0.7004479010992704 1104 88-89 0.5984888908376504 1104 90-91 0.573842800094031 1104 92-93 0.3766963071924927 1104 94-95 0.3713382309065203 1104 96-97 0.2204958190954862 1104 98-99 0.33729761589007623 1104 100-101 0.8167053327616074 1105 1 0.00883767807496838 1105 2 -0.008795144582251169 1105 3 0.09725374924483532 1105 4 0.11441172678232903 1105 5 0.04684970100844765 1105 6 -0.12966488775850138 1105 7 -0.016751100759584858 1105 8 -0.055805300227447674 1105 9 0.05324565658266778 1105 10-11 0.1653549664915488 1105 12-13 0.13428410509602884 1105 14-15 0.2516333428677626 1105 16-17 0.19723916458584512 1105 18-19 0.15132294164597226 1105 20-21 -0.08013299814161456 1105 22-23 0.1320080851659995 1105 24-25 -0.12480730285101771 1105 26-27 -0.21963556218877756 1105 28-29 -0.17323604423103234 1105 30-31 -0.15608963672658405 1105 32-33 -0.17909465028557747 1105 34-35 0.18190300135734816 1105 36-37 0.21182191304503561 1105 38-39 0.17944210972572705 1105 40-41 0.3342128178907018 1105 42-43 0.29969301941284243 1105 44-45 0.3684923523725203 1105 46-47 0.1829423145999982 1105 48-49 0.2808455313673832 1105 50-51 0.40133297192267037 1105 52-53 0.28079994364968286 1105 54-55 0.5067589425077941 1105 56-57 0.28445376046887105 1105 58-59 0.7422266681246867 1105 60-61 0.9372166197215179 1105 62-63 0.7468824955048277 1105 64-65 0.6636132136907946 1105 66-67 0.7403318182776175 1105 68-69 0.8665743547636353 1105 70-71 1.0054410619673568 1105 72-73 0.9341926759067185 1105 74-75 0.9645670693810402 1105 76-77 0.9133779930662342 1105 78-79 0.886895570460009 1105 80-81 0.9551863931344613 1105 82-83 1.220786213631932 1105 84-85 1.116815418337712 1105 86-87 0.7595624859248211 1105 88-89 0.907290707661609 1105 90-91 0.9244705326358478 1105 92-93 0.8783360710359354 1105 94-95 0.8544754992910697 1105 96-97 0.6804395495057065 1105 98-99 0.7196070757134727 1105 100-101 0.9078139353619292 1106 1 -0.0953428973502426 1106 2 0.16885546740312307 1106 3 -0.11229004347492477 1106 4 0.02690258674100221 1106 5 0.11659377985124308 1106 6 0.18386873169787066 1106 7 0.021983599606016924 1106 8 -5.136136911261246E-4 1106 9 0.028785316414172257 1106 10-11 -0.08809917434456338 1106 12-13 -0.08839909312365535 1106 14-15 -0.12548635193433455 1106 16-17 -0.12691915682569288 1106 18-19 -0.15373507774680917 1106 20-21 0.08720446807996751 1106 22-23 0.13386152659025896 1106 24-25 0.10116948939187154 1106 26-27 0.2750361911095851 1106 28-29 0.37282340577691997 1106 30-31 0.5062629304328468 1106 32-33 0.42020911700101493 1106 34-35 0.22596276927976788 1106 36-37 0.38882715863397266 1106 38-39 0.29061998287785684 1106 40-41 0.37752548678277265 1106 42-43 0.3081463654627612 1106 44-45 0.6113058955294051 1106 46-47 0.6940438882766813 1106 48-49 0.5834269969531363 1106 50-51 0.4871825983731597 1106 52-53 0.47651127730092924 1106 54-55 0.5899666996211224 1106 56-57 0.410455985870243 1106 58-59 0.6200171624817088 1106 60-61 0.5356270473164884 1106 62-63 0.766542327010157 1106 64-65 0.7291798962531857 1106 66-67 0.6234950673636135 1106 68-69 0.5648162876836764 1106 70-71 0.5411142458808875 1106 72-73 0.753461472600403 1106 74-75 0.7149151857377412 1106 76-77 0.6300335327260669 1106 78-79 0.633485937651237 1106 80-81 0.8360129707850703 1106 82-83 0.9180394325160535 1106 84-85 0.9955151481103997 1106 86-87 0.9484704496825671 1106 88-89 1.0240320842313118 1106 90-91 1.1087337658261198 1106 92-93 1.0437724086611162 1106 94-95 1.1622644040756853 1106 96-97 1.4090009864791604 1106 98-99 1.30631507126267 1106 100-101 1.4444885498906217 1107 1 -0.1518103689483894 1107 2 -0.011866933126704282 1107 3 -0.06136742897597003 1107 4 -0.021195635277443614 1107 5 -0.10228848618370279 1107 6 -0.028896055097177964 1107 7 -0.07108889517211736 1107 8 0.04523706133052485 1107 9 0.04420739599031265 1107 10-11 0.03939687741558373 1107 12-13 0.15988660660634224 1107 14-15 0.10041613927764814 1107 16-17 0.09422404965451392 1107 18-19 0.15760426710010478 1107 20-21 0.24869104406447917 1107 22-23 0.2947184220001091 1107 24-25 0.18417015167726447 1107 26-27 0.15614984947221444 1107 28-29 0.18929699077870055 1107 30-31 0.12678155084143583 1107 32-33 0.20646007222034513 1107 34-35 0.045799745182769414 1107 36-37 0.18824332858659432 1107 38-39 0.37320289591468736 1107 40-41 0.3049481776992735 1107 42-43 0.2904215705165001 1107 44-45 0.5102177096819389 1107 46-47 0.1780237140446772 1107 48-49 0.21867280672439193 1107 50-51 -0.009405103496526124 1107 52-53 0.11614243528146773 1107 54-55 0.1420967113384819 1107 56-57 0.3086718909087054 1107 58-59 0.32841188457656756 1107 60-61 0.3882736416412129 1107 62-63 0.46905289810394635 1107 64-65 0.6230807572242014 1107 66-67 0.44930789532224225 1107 68-69 0.5801935356331427 1107 70-71 0.7784681610064617 1107 72-73 0.8254019688050072 1107 74-75 0.7544811340794837 1107 76-77 0.45253493371439646 1107 78-79 0.5928470869711191 1107 80-81 0.6713932754363512 1107 82-83 0.7322439258677278 1107 84-85 0.7141661237077308 1107 86-87 0.7340947810290466 1107 88-89 0.7956210597267521 1107 90-91 0.8424797539049855 1107 92-93 0.9389305929802383 1107 94-95 0.9116046659331403 1107 96-97 0.9023669246318953 1107 98-99 1.10252883660986 1107 100-101 1.2720304521780506 1108 1 -0.19464453969479933 1108 2 -0.3522060362331523 1108 3 -0.20676964661512542 1108 4 -0.3452324611807853 1108 5 -0.2510159104632592 1108 6 -0.13987639183919498 1108 7 -0.2589603872937545 1108 8 -0.3130946472604563 1108 9 -0.13911720248964343 1108 10-11 -0.2405992947510569 1108 12-13 -0.1891456133760272 1108 14-15 -0.175658292405366 1108 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-1.3068606383539105 2316 64-65 -1.4108657247324672 2316 66-67 -1.7167126281763352 2316 68-69 -1.70286844729441 2316 70-71 -1.5536141291716383 2316 72-73 -2.012759789180013 2316 74-75 -2.0263994189804286 2316 76-77 -1.900311626046971 2316 78-79 -1.9929142064150334 2316 80-81 -1.9028801200367056 2316 82-83 -2.0555532201041515 2316 84-85 -2.2051031363004263 2316 86-87 -2.335585015831569 2316 88-89 -2.201526472704881 2316 90-91 -2.5796508911160743 2316 92-93 -2.4077997145155443 2316 94-95 -2.426648495858288 2316 96-97 -2.686600190062048 2316 98-99 -2.9974858771736095 2316 100-101 -2.8783867727578 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2320.0 3 312.0 4 217.0 5 264.0 6 336.0 7 393.0 8 553.0 9 677.0 10 1001.0 11 1595.0 12 2684.0 13 4042.0 14 4976.0 15 6891.0 16 9581.0 17 13675.0 18 10264.0 19 5287.0 20 4620.0 21 4540.0 22 4911.0 23 5403.0 24 5872.0 25 6464.0 26 7451.0 27 8651.0 28 10179.0 29 12248.0 30 14922.0 31 18278.0 32 22753.0 33 29759.0 34 40716.0 35 59362.0 36 82289.0 37 130736.0 38 133097.0 39 19898.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.196494859506814 23.441466401771546 15.688758939115482 19.67327979960616 2 14.130901130974097 23.419919737322147 41.39073330901131 21.05844582269245 3 17.013405483026432 29.844346960635804 32.715303498618006 20.426944057719755 4 10.424668729204365 19.995621971863873 41.820238164730604 27.759471134201156 5 9.871056137345569 41.48528388223604 35.737016971212704 12.90664300920569 6 24.425005358791115 42.34276140328876 19.36995198231526 13.862281255604858 7 23.517582516937956 37.83310827167227 23.009370925922145 15.639938285467627 8 24.38545298644256 35.557033258524626 21.44516433855536 18.612349416477457 9 26.339273996692825 17.202551248064253 23.469849019076033 32.988325736166885 10-11 21.91655662073054 29.77248747080532 29.24470448746789 19.066251420996256 12-13 23.79687411130054 29.18966651256466 29.16764499564674 17.84581438048806 14-15 21.29556521314375 29.697885658918967 29.38883797633915 19.617711151598133 16-17 17.311465655968345 33.00910488887398 30.25423253012663 19.425196925031045 18-19 17.026776985309073 30.626837641607302 35.24801645885323 17.09836891423039 20-21 17.994845304451484 29.6700484483054 35.35012923193578 16.984977015307333 22-23 18.27042113045114 29.55758045019153 34.75926549693277 17.412732922424563 24-25 17.683806107794034 30.48349116782947 34.928445931660946 16.904256792715554 26-27 17.355270669603502 30.381694194057353 35.51233902254614 16.750696113793005 28-29 16.64832989564445 30.312979152950543 36.05760152118644 16.98108943021856 30-31 17.51951911663055 29.971650980403595 35.51261560537698 16.996214297588875 32-33 16.94410660693505 30.54281981059711 35.30122467312398 17.211848909343857 34-35 17.45101541624593 30.687697988852474 34.927840012248424 16.93344658265317 36-37 17.83384089548691 30.106176121272483 34.41723431727726 17.642748665963346 38-39 16.78430417692137 31.4923949937597 34.34667805862387 17.376622770695064 40-41 16.882336731078507 31.07631877080457 35.01626456046319 17.025079937653732 42-43 16.883801392257507 30.84982529347998 34.29941336809239 17.966959946170125 44-45 17.417188171019887 31.09638771196482 33.5455806835225 17.94084343349279 46-47 18.024065818299793 31.428529768782965 32.68547849131159 17.86192592160565 48-49 17.58916925553578 31.53362880829877 32.62580226994183 18.251399666223627 50-51 17.616885190678058 31.590974382306918 32.18209021826152 18.6100502087535 52-53 17.763700957586753 32.501509446069754 31.696106941701842 18.038682654641654 54-55 18.26521542254348 31.72607098768358 31.43309020351883 18.57562338625411 56-57 18.262442884760617 32.59716515963108 30.82145346073883 18.318938494869467 58-59 18.316489972338744 31.69681221322857 31.400957028473965 18.585740785958723 60-61 18.445729331134835 31.967801050555238 31.649908225850425 17.936561392459506 62-63 18.22621137282116 31.547886330104564 32.08162515526682 18.144277141807454 64-65 18.272226425767098 31.684766207581248 31.67055190843367 18.372455458217978 66-67 18.205034180139577 31.940806428213687 30.869583284428188 18.984576107218544 68-69 18.11950697341479 31.460586520567436 31.600543206888787 18.819363299128987 70-71 18.120484913871657 32.00963704357081 31.78620634353655 18.083671699020986 72-73 18.241038783031556 31.15612731070534 32.067687125666644 18.535146780596452 74-75 17.483798068161107 32.26848915618735 31.728157463094586 18.519555312556953 76-77 17.46779204058228 31.99179372324813 32.12411774869808 18.416296487471502 78-79 17.35551469676932 31.713716644479472 31.690387281998046 19.240381376753167 80-81 17.257619969923233 31.71553473006357 31.663122720642534 19.363722579370663 82-83 17.308499873144928 32.14492973395429 31.56132349210145 18.985246900799332 84-85 17.308051164974096 31.42878802457365 31.41501126924008 19.84814954121218 86-87 16.99065255693353 31.792095021281963 31.682386670544727 19.534865751239778 88-89 17.625814315624947 31.13470544665613 31.69714457346483 19.542335664254097 90-91 17.29237090134506 30.726125031984434 32.45813228397658 19.523371782693925 92-93 17.59645450721281 30.538757138264156 32.4188189904619 19.445969364061135 94-95 17.237135918968118 30.16438641779914 32.570075096212236 20.02840256702051 96-97 17.697397100697053 29.906296791463344 32.02136205712586 20.374944050713744 98-99 17.54022733767635 30.07704286055884 31.82173413506121 20.560995666703604 100-101 17.36443929024654 29.78206343988285 32.61394855213122 20.23954871773939 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2104.0 1 1325.0 2 667.0 3 778.5 4 679.5 5 731.0 6 1292.5 7 2558.0 8 3978.0 9 4228.5 10 3286.0 11 2546.0 12 2465.5 13 2428.0 14 2334.5 15 2309.5 16 2319.5 17 2400.0 18 2484.0 19 2676.0 20 2988.0 21 3335.5 22 3699.0 23 4122.5 24 4645.0 25 5728.5 26 8318.0 27 11580.5 28 12560.5 29 13314.0 30 15507.5 31 17558.5 32 20744.0 33 25091.5 34 30238.5 35 33637.5 36 38106.0 37 42529.5 38 39804.0 39 34819.0 40 30767.5 41 27312.5 42 24685.0 43 22960.5 44 22171.0 45 20994.0 46 18561.0 47 16364.0 48 17240.0 49 15689.5 50 11182.0 51 8178.0 52 6211.0 53 5113.5 54 4273.5 55 3631.5 56 3277.5 57 2968.5 58 2694.5 59 2479.0 60 2584.0 61 2687.0 62 2025.0 63 993.5 64 340.0 65 203.5 66 156.0 67 111.5 68 81.0 69 60.0 70 45.0 71 36.0 72 33.0 73 32.5 74 41.0 75 46.0 76 31.5 77 19.0 78 22.0 79 22.0 80 12.0 81 7.0 82 4.5 83 0.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 1.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31547439753557455 2 0.28651710735587926 3 0.28768121952390724 4 0.2879722475659142 5 0.2738573875285753 6 0.2080850500349961 7 0.21550626510617432 8 0.21390561087513588 9 0.20910364818202057 10-11 0.2212540689358123 12-13 0.22205439605133154 14-15 0.20408341445740003 16-17 0.21659762026370052 18-19 0.20190070414234765 20-21 0.20903089117151885 22-23 0.2069209378669682 24-25 0.19993626485880048 26-27 0.21063154540255724 28-29 0.2093219192135258 30-31 0.21550626510617432 32-33 0.21608832119018828 34-35 0.20612061075144894 36-37 0.20728472291947692 38-39 0.1971714984597341 40-41 0.19964523681679347 42-43 0.19709874144923234 44-45 0.2128142557176097 46-47 0.2024827602263616 48-49 0.20815780704549788 50-51 0.21565177912717778 52-53 0.22365505028236998 54-55 0.2195079006837704 56-57 0.19280607782962927 58-59 0.18065565707583753 60-61 0.18858617122052795 62-63 0.1896047693675524 64-65 0.187713087094507 66-67 0.19899042372227776 68-69 0.1880768721470157 70-71 0.19266056380862578 72-73 0.20612061075144894 74-75 0.19557084422869567 76-77 0.20408341445740003 78-79 0.20197346115284937 80-81 0.19025958246206814 82-83 0.20328308734188083 84-85 0.18640346090547555 86-87 0.19055061050407512 88-89 0.198917666711776 90-91 0.19477051711317644 92-93 0.2023372462053581 94-95 0.19869939568027076 96-97 0.19353364793464675 98-99 0.198917666711776 100-101 0.1837114515169109 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 687219.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.910275560858736 #Duplication Level Percentage of deduplicated Percentage of total 1 76.9364495560737 26.08936205120566 2 11.537994046254228 7.825131150560566 3 3.8298489277872583 3.896136974931759 4 1.8424296717016442 2.49909191475621 5 1.0545950137600717 1.788080376085582 6 0.6815403249571914 1.3866732135081339 7 0.47921716731204134 1.1375270337931822 8 0.3332930535002878 0.9041647429371832 9 0.2611535787320903 0.7970210836658639 >10 2.317438678634766 16.748965764698916 >50 0.4023525296012569 9.55378073395648 >100 0.3034838524434941 19.23248464654676 >500 0.015475097291736241 3.5146123383752252 >1k 0.0038687743229340603 2.3163993475642117 >5k 8.597276273186802E-4 2.3105686274142796 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9154 1.3320353482659821 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6697 0.9745073986603979 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4790 0.6970121606067353 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2755 0.4008911278646254 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1545 0.2248191624503979 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1402 0.2040106574468983 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1256 0.1827656103803882 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1070 0.15570000247373836 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1041 0.15148009586463704 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1029 0.14973392761259513 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1003 0.14595056306650428 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 942 0.13707420778529114 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 934 0.1359100956172632 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 922 0.13416392736522129 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 921 0.13401841334421777 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 885 0.12877990858809202 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 864 0.12572411414701865 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 858 0.12485103002099766 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 796 0.11582916071878105 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 794 0.11553813267677407 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 730 0.10622523533255047 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 723 0.105206637185526 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 721 0.10491560914351902 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 717 0.10433355305950506 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 697 0.10142327263943517 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.455140210034938E-4 0.0 18-19 0.0 0.0 0.0 1.455140210034938E-4 0.0 20-21 0.0 0.0 0.0 2.1827103150524072E-4 0.0 22-23 0.0 0.0 0.0 5.092990735122283E-4 0.0 24-25 0.0 0.0 0.0 0.0018916822730454194 0.0 26-27 0.0 0.0 0.0 0.002692009388564635 0.0 28-29 0.0 0.0 0.0 0.005020233724620536 0.0 30-31 0.0 0.0 0.0 0.009967710438739324 0.0 32-33 0.0 0.0 0.0 0.015206215194865102 0.0 34-35 0.0 0.0 0.0 0.020226448919485638 0.0 36-37 0.0 0.0 0.0 0.02786593502216906 0.0 38-39 0.0 0.0 0.0 0.040234626807466034 0.0 40-41 0.0 0.0 0.0 0.05318537467677698 0.0 42-43 0.0 0.0 0.0 0.06824607585063858 0.0 44-45 0.0 0.0 0.0 0.07530350586930804 0.0 46-47 0.0 0.0 0.0 0.08723565559159452 0.0 48-49 0.0 0.0 0.0 0.10142327263943517 0.0 50-51 0.0 0.0 0.0 0.11983079629637713 0.0 52-53 0.0 0.0 0.0 0.1722158438576349 0.0 54-55 0.0 0.0 0.0 0.20684818085646642 0.0 56-57 0.0 0.0 0.0 0.30106850945622865 0.0 58-59 0.0 0.0 0.0 0.43988888549356175 0.0 60-61 0.0 0.0 0.0 0.5700511772811869 0.0 62-63 0.0 0.0 0.0 0.6302212249661316 0.0 64-65 0.0 0.0 0.0 0.7325175817315877 0.0 66-67 0.0 0.0 0.0 0.9707240341143071 0.0 68-69 0.0 0.0 0.0 1.3018411889077572 0.0 70-71 0.0 0.0 0.0 1.6031279693954912 0.0 72-73 0.0 0.0 0.0 1.9413025542076108 0.0 74-75 0.0 0.0 0.0 2.360237420676669 0.0 76-77 0.0 0.0 0.0 2.6388240138878585 0.0 78-79 0.0 0.0 0.0 3.0056648608376664 0.0 80-81 0.0 0.0 0.0 3.5047779528796497 0.0 82-83 0.0 0.0 0.0 3.9294606231783464 0.0 84-85 0.0 0.0 0.0 4.42399002355872 0.0 86-87 0.0 0.0 0.0 5.083314052725551 0.0 88-89 0.0 0.0 0.0 5.9772794407605145 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 190 0.0 42.49651 9 GGCATTC 240 0.0 33.64552 7 GGTATCA 4660 0.0 33.272976 1 GTATCAA 7410 0.0 33.184273 1 TAGGCAT 275 0.0 32.84662 5 TTAGGCA 270 0.0 31.694107 4 GGTGTAC 75 3.077329E-4 31.666374 8 CATATAG 210 1.8189894E-12 29.432388 3 GTGTACG 65 0.0058447956 29.228367 9 CTCCCCG 190 3.9108272E-10 27.523829 5 GTAGGAC 175 4.9039954E-9 27.16836 3 ACGTGGA 70 0.0083896285 27.142609 8 TCAACGC 9045 0.0 27.068764 4 ATCAACG 9050 0.0 27.055782 3 TATCAAC 9035 0.0 27.048079 2 GTACATG 4700 0.0 27.019255 1 CAACGCA 9075 0.0 26.874508 5 CCGGGTC 195 5.347829E-10 26.792671 9 AACGCAG 9185 0.0 26.581137 6 ACATGGG 4690 0.0 25.749117 3 >>END_MODULE