FastQCFastQC Report
Thu 26 May 2016
SRR937197_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937197_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687219
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108161.5738796511737887No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86371.256804599407176No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60070.8741027241679873No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28590.41602458604898873No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21040.30616150019135097No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18040.2625072938903028No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15810.23005766720652368No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12360.17985532996031833No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT11610.1689417783850563No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC11340.16501289981796197No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA11040.16064747918785716No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10410.15148009586463704No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA9730.14158514243639947No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9650.1404210302683715No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA8940.13008953477712346No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8770.12761579642006404No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT8730.12703374033605008No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA8390.1220862636219313No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT8320.12106766547490683No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT8230.11975803928587539No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC7910.11510159061376359No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA7750.11277336627770768No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG7570.1101541138996448No Hit
GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG7200.10477009512251552No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACT459.6239644E-442.22087
CTTTGCG608.281813E-539.587762
TGCGTAC500.00161563137.998726
TATATCG550.002577493434.5493164
GTGCGTA550.002577493434.5493165
CATATAG2450.032.9628683
GTATCAA85450.032.4859731
GGTATCA55300.032.2330361
ATGGGCG600.003950508331.6655987
TCCCTCG600.003950508331.6655989
GACATAT2600.031.0792581
CGGATTA650.005843420529.2297849
GTCGGGA2002.3646862E-1128.5031872
GGACCAC2350.028.2969196
GGTATGG1354.19539E-728.1676921
GCAGATC856.40035E-427.9443023
CATTCCG2053.092282E-1127.8039429
GTATTGA700.00836090227.1617051
GACAGTA700.00839064227.1419457
ATCGGAT700.00839064227.1419457
Produced by FastQC (version 0.11.5)