##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937197_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 687219 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.101875821244754 33.0 31.0 34.0 31.0 34.0 2 32.31988929293282 33.0 31.0 34.0 31.0 34.0 3 32.36180169640246 34.0 31.0 34.0 30.0 34.0 4 36.02219234334324 37.0 35.0 37.0 35.0 37.0 5 35.94574946268948 37.0 35.0 37.0 35.0 37.0 6 36.04212921936093 37.0 35.0 37.0 35.0 37.0 7 35.99344895877442 37.0 35.0 37.0 35.0 37.0 8 35.99080351387258 37.0 35.0 37.0 35.0 37.0 9 37.64463875416716 39.0 37.0 39.0 35.0 39.0 10-11 37.591070677615136 39.0 37.0 39.0 35.0 39.0 12-13 37.53514672906307 39.0 37.0 39.0 35.0 39.0 14-15 38.6436296144315 40.0 38.0 41.0 34.0 41.0 16-17 38.639328219970636 40.0 38.0 41.0 34.5 41.0 18-19 38.78306042178694 40.0 38.0 41.0 35.0 41.0 20-21 38.7570970825894 40.0 38.0 41.0 35.0 41.0 22-23 38.64810344300725 40.0 38.0 41.0 34.0 41.0 24-25 38.50790504919101 40.0 38.0 41.0 34.0 41.0 26-27 38.37077190822722 40.0 38.0 41.0 34.0 41.0 28-29 38.268679271091166 40.0 38.0 41.0 34.0 41.0 30-31 38.118075169632974 40.0 37.0 41.0 34.0 41.0 32-33 37.841523590005515 40.0 36.0 41.0 33.5 41.0 34-35 37.65803695765105 40.0 36.0 41.0 33.0 41.0 36-37 37.408943146216856 40.0 35.0 41.0 33.0 41.0 38-39 37.20331364528629 40.0 35.0 41.0 33.0 41.0 40-41 36.97091029206119 40.0 35.0 41.0 33.0 41.0 42-43 36.612998913010266 40.0 35.0 41.0 31.5 41.0 44-45 36.05439605133152 39.0 35.0 41.0 30.5 41.0 46-47 35.3625038015538 39.0 35.0 41.0 26.5 41.0 48-49 34.79583655283105 39.0 35.0 41.0 22.5 41.0 50-51 34.50744231460422 39.0 35.0 41.0 19.0 41.0 52-53 34.283455492353966 39.0 35.0 41.0 14.0 41.0 54-55 34.06656029591731 39.0 35.0 40.0 8.5 41.0 56-57 33.850172943413966 38.5 34.0 40.0 4.5 41.0 58-59 33.646996081307414 38.0 34.0 40.0 2.0 41.0 60-61 33.37238856900056 38.0 34.0 40.0 2.0 41.0 62-63 33.041927682441845 38.0 33.5 40.0 2.0 41.0 64-65 32.723354563829 37.5 33.0 40.0 2.0 41.0 66-67 32.30879894182204 37.0 33.0 39.5 2.0 41.0 68-69 31.899763394201848 36.0 32.0 39.0 2.0 40.5 70-71 31.527750978945576 36.0 32.0 39.0 2.0 40.0 72-73 31.086871870539085 35.0 31.0 38.5 2.0 40.0 74-75 30.61162089523136 35.0 31.0 37.5 2.0 39.5 76-77 29.226394351727762 34.0 29.5 36.0 2.0 38.5 78-79 29.78880458776605 35.0 30.0 36.0 2.0 39.0 80-81 29.58613556959281 35.0 30.0 36.0 2.0 38.5 82-83 29.213156213666966 34.5 30.0 36.0 2.0 37.0 84-85 28.798208431373403 34.0 29.5 35.0 2.0 37.0 86-87 28.404188475580565 34.0 29.0 35.0 2.0 36.5 88-89 27.966166534976477 34.0 29.0 35.0 2.0 36.0 90-91 27.506441905709824 34.0 27.5 35.0 2.0 36.0 92-93 27.152348814570026 34.0 26.5 35.0 2.0 35.0 94-95 26.731552823772333 34.0 26.0 35.0 2.0 35.0 96-97 26.27329861368792 33.0 25.0 35.0 2.0 35.0 98-99 25.861210181907076 33.0 24.5 35.0 2.0 35.0 100-101 24.11326811394912 31.0 10.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37201878029718927 1101 2 0.36176434056215356 1101 3 0.211894822777559 1101 4 0.1855163116327958 1101 5 0.1626452024875391 1101 6 0.16579235708626072 1101 7 0.23840556784299594 1101 8 0.21562025084733705 1101 9 0.29511182748656495 1101 10-11 0.20594643270835888 1101 12-13 0.2565096826357802 1101 14-15 0.247393570311786 1101 16-17 0.3706651325283232 1101 18-19 0.3426003862147624 1101 20-21 0.4826935939565047 1101 22-23 0.25277781873970184 1101 24-25 0.5096037556175332 1101 26-27 0.36006249430438686 1101 28-29 0.351361290523613 1101 30-31 0.32506898616119884 1101 32-33 0.4792642848350468 1101 34-35 0.49647404348775837 1101 36-37 0.17072400652747177 1101 38-39 -0.009336640825324594 1101 40-41 0.06376354520447336 1101 42-43 0.09607921697870836 1101 44-45 0.1250043233805016 1101 46-47 0.6110077105416707 1101 48-49 0.5876773545156269 1101 50-51 0.6013303237435466 1101 52-53 0.4006837520636566 1101 54-55 0.29668996874475795 1101 56-57 0.4320464170893459 1101 58-59 0.20211698182344406 1101 60-61 0.10903905987868256 1101 62-63 0.46881049027832944 1101 64-65 0.36651466635643004 1101 66-67 0.3542963909992096 1101 68-69 0.24837384191453182 1101 70-71 0.20694600305309052 1101 72-73 0.24189948796463767 1101 74-75 0.5269451919750345 1101 76-77 0.21762150562514293 1101 78-79 0.14062588424967615 1101 80-81 0.28457231109999626 1101 82-83 0.31254091710865595 1101 84-85 0.342931234519245 1101 86-87 0.4227046243105903 1101 88-89 0.22450371802340996 1101 90-91 0.1392194470122341 1101 92-93 0.1833502434128782 1101 94-95 0.2437233461878101 1101 96-97 0.39799870678574933 1101 98-99 0.725831232810247 1101 100-101 0.95763577368227 1102 1 0.033116189112440964 1102 2 -0.03629347990230514 1102 3 0.0700808967312696 1102 4 0.05352548088677622 1102 5 -0.0851761324707283 1102 6 0.0855033514860537 1102 7 -0.0571287733345045 1102 8 0.07516043403897044 1102 9 0.14594702272010096 1102 10-11 0.14774294877873473 1102 12-13 0.1485862091521426 1102 14-15 0.09098041223887776 1102 16-17 0.01747185277903185 1102 18-19 -0.005244162044718337 1102 20-21 0.17334346966862313 1102 22-23 0.06757726548936205 1102 24-25 0.2683604898929417 1102 26-27 0.10709264257934592 1102 28-29 0.17681951141751995 1102 30-31 0.3162547022431852 1102 32-33 0.3097172343751353 1102 34-35 0.4069887017202589 1102 36-37 0.526471716632031 1102 38-39 0.01204667848330132 1102 40-41 0.15391143327767054 1102 42-43 0.4090941411969524 1102 44-45 0.4788601494354978 1102 46-47 0.3372590525606469 1102 48-49 0.2988593951582459 1102 50-51 0.3071259112818794 1102 52-53 0.34436247997475533 1102 54-55 0.2627453518125833 1102 56-57 0.3516336072159518 1102 58-59 0.5035813542421792 1102 60-61 0.5013989821141251 1102 62-63 0.5532662834478188 1102 64-65 0.4329791838582935 1102 66-67 0.45377989504703464 1102 68-69 0.42806800667093725 1102 70-71 0.2799228187684939 1102 72-73 0.3339634406095051 1102 74-75 0.36724638455838843 1102 76-77 0.5710943122099827 1102 78-79 0.5448690822688071 1102 80-81 0.5518096380883613 1102 82-83 0.46752548993555365 1102 84-85 0.5656183675798232 1102 86-87 0.576454056068318 1102 88-89 0.5941571627805935 1102 90-91 0.618990989439375 1102 92-93 0.6818394443917022 1102 94-95 0.402679327965366 1102 96-97 0.3247085115038537 1102 98-99 0.5049141139678177 1102 100-101 0.7490352455796696 1103 1 -0.004093467715748034 1103 2 1.711453906594329E-4 1103 3 0.11744089368254862 1103 4 0.05849108748726195 1103 5 -0.09227393929605654 1103 6 -0.030652971754740577 1103 7 -0.048144569527408976 1103 8 0.0628131083401513 1103 9 0.16826938700432237 1103 10-11 0.017555833441754487 1103 12-13 0.00826685548128836 1103 14-15 0.042555267900930005 1103 16-17 0.10846573627972589 1103 18-19 0.1531074318654575 1103 20-21 0.09509800940681146 1103 22-23 -0.11607396066867892 1103 24-25 -0.07147964272648011 1103 26-27 0.04434517206620825 1103 28-29 -0.2916443135858131 1103 30-31 -0.03923204534165592 1103 32-33 -0.032488516277489055 1103 34-35 0.0647305511514773 1103 36-37 0.011330999038996481 1103 38-39 0.2763956809794408 1103 40-41 0.23550858272223252 1103 42-43 0.3136605557088714 1103 44-45 0.40854041467434143 1103 46-47 0.505427532581983 1103 48-49 0.4930849243974009 1103 50-51 0.37967121339121235 1103 52-53 0.6663456738925433 1103 54-55 0.537744875449718 1103 56-57 0.4462573758179289 1103 58-59 0.5708980974444771 1103 60-61 0.3376816367891564 1103 62-63 0.4740480901969235 1103 64-65 0.32083288329236836 1103 66-67 0.4825426853902002 1103 68-69 0.5485620902440438 1103 70-71 0.4683624445378207 1103 72-73 0.531394891991912 1103 74-75 0.8689974944838816 1103 76-77 0.6110030410832898 1103 78-79 0.5936448296821553 1103 80-81 0.759568636583051 1103 82-83 0.64693232288462 1103 84-85 0.6797101341239049 1103 86-87 0.4681339021078408 1103 88-89 0.5227284552483411 1103 90-91 0.4379416573001258 1103 92-93 0.8541408487094593 1103 94-95 0.7541646274070679 1103 96-97 0.7027377030207838 1103 98-99 0.8081733887529765 1103 100-101 1.0239918965581154 1104 1 0.06631103165307195 1104 2 0.10340233829818146 1104 3 0.11314997718352515 1104 4 0.06363873212152527 1104 5 0.03898008514343587 1104 6 0.05740994956087775 1104 7 -0.010595866988509783 1104 8 0.08196671187328519 1104 9 0.056289017174250944 1104 10-11 -0.012677938744957373 1104 12-13 -0.0019520376477899504 1104 14-15 0.050751482290323224 1104 16-17 0.08375977046881644 1104 18-19 -0.02264825328058606 1104 20-21 -0.17805093032391284 1104 22-23 0.018445089254377933 1104 24-25 -0.017146526455192657 1104 26-27 -0.01682176077430597 1104 28-29 0.06526844566084833 1104 30-31 -0.017912065568438607 1104 32-33 -0.013245783914989318 1104 34-35 0.1636189873225078 1104 36-37 0.25260819697411563 1104 38-39 0.13059728675353455 1104 40-41 0.2582146067397204 1104 42-43 0.4782642234511414 1104 44-45 0.6540130339341701 1104 46-47 0.6702512939641068 1104 48-49 0.4406689874351244 1104 50-51 0.2555793270250106 1104 52-53 0.24731466797366863 1104 54-55 0.3724627372309044 1104 56-57 0.45288655987078386 1104 58-59 0.32741359676601434 1104 60-61 0.4029357630901558 1104 62-63 0.27120815466071946 1104 64-65 -0.058815003262992605 1104 66-67 -0.004915615050350652 1104 68-69 0.03400225933416223 1104 70-71 0.028267931599589957 1104 72-73 0.11581990190521552 1104 74-75 0.10101075103303359 1104 76-77 0.19374642645087903 1104 78-79 0.23176723084919715 1104 80-81 0.31161805397154296 1104 82-83 0.12192622938302122 1104 84-85 0.03489488545447017 1104 86-87 -0.024925872414410577 1104 88-89 0.1602751569359704 1104 90-91 0.35651701481586784 1104 92-93 0.313871897919487 1104 94-95 0.08985290205482954 1104 96-97 0.4875288687167334 1104 98-99 0.556079770740947 1104 100-101 0.8988713907429187 1105 1 -0.06710290509807493 1105 2 0.04011337504532264 1105 3 0.01419686659831143 1105 4 -0.07502779702272733 1105 5 -0.14290095414404647 1105 6 -0.03337776739506637 1105 7 -0.03615975665733373 1105 8 -0.0680511469408458 1105 9 -0.18265427583827432 1105 10-11 -0.1858224843575158 1105 12-13 -0.1719536229756784 1105 14-15 -0.25619187184327785 1105 16-17 -0.05437003923453432 1105 18-19 0.17861389692063767 1105 20-21 0.08347182486135551 1105 22-23 -0.12383904352351749 1105 24-25 -0.20207979943146626 1105 26-27 -0.31392939689762756 1105 28-29 -0.32301941932269074 1105 30-31 -0.39797341584758783 1105 32-33 -0.26777208603264313 1105 34-35 -0.39186080356702035 1105 36-37 -0.3863878129656939 1105 38-39 -0.30924047499269136 1105 40-41 -0.25259236335487856 1105 42-43 -0.20589930598533357 1105 44-45 -0.3678129199843525 1105 46-47 0.03562735092894087 1105 48-49 0.12914441771794571 1105 50-51 0.11768445145414574 1105 52-53 0.31869644357909976 1105 54-55 0.5430258724364592 1105 56-57 0.5050285670486829 1105 58-59 0.45839892579794395 1105 60-61 0.3760090644495335 1105 62-63 0.2980775522934209 1105 64-65 0.15201737027845752 1105 66-67 0.3798931237353713 1105 68-69 0.5823423446362561 1105 70-71 0.369371281607517 1105 72-73 0.24472806939988345 1105 74-75 0.35662481728445883 1105 76-77 0.5326417776201069 1105 78-79 0.6879816206603131 1105 80-81 0.654706883904062 1105 82-83 0.6322003001039249 1105 84-85 0.34245040535606464 1105 86-87 0.2965136817073457 1105 88-89 0.5906076364842825 1105 90-91 0.544956154460337 1105 92-93 0.5549658887627835 1105 94-95 0.6099044347612868 1105 96-97 0.7245974637533195 1105 98-99 0.7768080283510024 1105 100-101 1.3303852260712787 1106 1 -0.19819176219551338 1106 2 0.011145007536178753 1106 3 0.032070018720389726 1106 4 0.032567179928477685 1106 5 -5.452078398064941E-4 1106 6 -0.12226665628395494 1106 7 -0.11543491164064079 1106 8 -0.08893177005322883 1106 9 -0.1995800428069643 1106 10-11 -0.23359867256289135 1106 12-13 -0.33236691180098177 1106 14-15 -0.2533656121071459 1106 16-17 -0.010370675063498425 1106 18-19 -0.13333015013071048 1106 20-21 -0.07376867744353177 1106 22-23 -0.05464495673286507 1106 24-25 -0.006594185061729263 1106 26-27 0.04785410334081064 1106 28-29 -0.07646720186919254 1106 30-31 -0.010209944221458045 1106 32-33 0.09799488265277745 1106 34-35 -0.1507735360419744 1106 36-37 0.07095760111791094 1106 38-39 0.22656623300285617 1106 40-41 -0.07885209948586436 1106 42-43 -0.43675926465645176 1106 44-45 -0.16900827843293342 1106 46-47 -0.026594871293276867 1106 48-49 -0.1464397501408925 1106 50-51 0.17075709491306412 1106 52-53 0.3108789264911991 1106 54-55 0.25502714409438454 1106 56-57 0.3432005587829039 1106 58-59 0.39660906726988543 1106 60-61 0.4582598989750437 1106 62-63 0.5589073090888448 1106 64-65 0.2926373035164147 1106 66-67 0.13842117968831502 1106 68-69 0.32968934829227337 1106 70-71 0.33816161700729097 1106 72-73 0.2025726092186737 1106 74-75 0.18382240113440318 1106 76-77 0.25376719488286525 1106 78-79 0.3209270983268233 1106 80-81 0.3430139892927109 1106 82-83 0.3123687947788554 1106 84-85 0.10563908760054375 1106 86-87 0.42431212392638784 1106 88-89 0.05904190766533546 1106 90-91 0.0721378425862298 1106 92-93 0.19078531333757454 1106 94-95 -0.22380085851481724 1106 96-97 0.22182842862376972 1106 98-99 0.5780765071302092 1106 100-101 0.6844561210005438 1107 1 -0.05554975292351827 1107 2 0.025183420089035735 1107 3 -0.04161483532209331 1107 4 0.12937700213031889 1107 5 0.024538087284753374 1107 6 0.005136675521029588 1107 7 -0.08050292325610542 1107 8 0.01784086227648629 1107 9 0.052094520339402095 1107 10-11 0.00967097948255713 1107 12-13 0.030007126611565127 1107 14-15 -0.026447276990737123 1107 16-17 -0.0027645820378765507 1107 18-19 0.017434534774281474 1107 20-21 0.1513217499852786 1107 22-23 0.1337395763559357 1107 24-25 0.09143933676821803 1107 26-27 -0.05371092799038735 1107 28-29 -0.12436266266698937 1107 30-31 0.11516679427769816 1107 32-33 0.09668253867193499 1107 34-35 0.17252770192225597 1107 36-37 -0.21810653867525076 1107 38-39 -0.19316681503831035 1107 40-41 0.08924102802954792 1107 42-43 0.20797352865955787 1107 44-45 0.1614507362901847 1107 46-47 -0.06111726163410225 1107 48-49 0.15267380633166994 1107 50-51 -0.022565030513604256 1107 52-53 0.2894524656424551 1107 54-55 0.03312078106009153 1107 56-57 -0.014655674341547353 1107 58-59 0.2626073351388598 1107 60-61 0.15921970334612467 1107 62-63 -0.03764473125962553 1107 64-65 -0.022771530351846536 1107 66-67 0.24794790035672065 1107 68-69 0.11324225470625748 1107 70-71 0.09403099394956982 1107 72-73 -0.07297377623142509 1107 74-75 -0.01866371634483599 1107 76-77 -0.011906043692714974 1107 78-79 0.11137286734205176 1107 80-81 -0.17902788752220644 1107 82-83 -0.2255017029540909 1107 84-85 -0.0712512277491939 1107 86-87 -0.08618866572158268 1107 88-89 0.14210879849013835 1107 90-91 0.4139535234665601 1107 92-93 0.4633228852974298 1107 94-95 0.526974015396231 1107 96-97 0.15756025398420803 1107 98-99 0.12882692742671153 1107 100-101 0.6957027458922838 1108 1 -0.1782890506413075 1108 2 -0.08815085529674604 1108 3 -0.10860907389628238 1108 4 -0.041210212548527636 1108 5 -0.18669749797128787 1108 6 -0.1309070261727001 1108 7 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2316 96-97 -2.2844696685009076 2316 98-99 -2.405309050226176 2316 100-101 -2.830012934765108 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 10.0 5 17.0 6 51.0 7 81.0 8 195.0 9 445.0 10 816.0 11 1650.0 12 2726.0 13 3934.0 14 5045.0 15 7679.0 16 11154.0 17 16174.0 18 13215.0 19 5882.0 20 4210.0 21 3683.0 22 3936.0 23 4273.0 24 4797.0 25 5444.0 26 6267.0 27 7432.0 28 9138.0 29 11294.0 30 14041.0 31 17145.0 32 21557.0 33 27952.0 34 37639.0 35 55374.0 36 78557.0 37 136980.0 38 150378.0 39 18044.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.11342613825808 23.526062983298317 16.562344845861414 18.798166032582188 2 14.656317709492898 23.954518137595148 41.00745177301559 20.381712379896367 3 17.268730928568623 29.653283742154972 33.469534456992605 19.6084508722838 4 10.815184097063673 20.54061369083218 41.39393701280087 27.250265199303282 5 9.734667829038235 41.44659955377739 35.859647999347985 12.959084617836389 6 24.71599300950643 42.470886282247726 18.756029737245335 14.05709097100051 7 23.327643735112098 38.21707490625259 22.81092344652869 15.64435791210662 8 24.81799833822988 35.394102898784816 21.13780323303052 18.65009552995479 9 26.420835279583365 16.871477651229082 23.831558789847197 32.87612827934036 10-11 22.14279581909115 29.756889725109463 29.060750648628748 19.03956380717064 12-13 24.19433934807916 29.056752037252622 29.45365250291036 17.29525611175786 14-15 21.480634266514752 29.279312708175997 30.209074545377817 19.030978479931434 16-17 17.06086414956513 32.49619117050023 31.138472597527134 19.3044720824075 18-19 16.75593951855231 29.379208083594893 37.08490306583491 16.77994933201789 20-21 17.634043878297895 28.397497740894824 37.488267932056594 16.48019044875069 22-23 17.889704336260845 28.237826070823385 36.983541576781114 16.888928016134653 24-25 17.3678987338825 29.005018778584414 37.37251152834831 16.254570959184772 26-27 17.13421772355251 28.936920078199634 37.861995254763606 16.06686694348425 28-29 16.412387305943582 28.941936413304063 38.26450243632345 16.381173844428908 30-31 17.388052425791486 28.465089003650945 37.70406522520477 16.44279334535279 32-33 16.95667611052663 28.99992578784929 37.427952370350646 16.615445731273436 34-35 17.337268032461267 28.991413217620583 37.291751246691376 16.379567503226774 36-37 17.776574861870817 28.42281718054943 36.78739964989327 17.013208307686487 38-39 16.711266714104237 29.902622017144466 36.73559665841602 16.65051461033528 40-41 17.01459069088602 29.528796497186487 37.0512893269831 16.405323484944393 42-43 16.96786103848569 29.265614505647076 36.41227112077779 17.354253335089442 44-45 17.521707054083198 29.425990841347517 35.57097519131456 17.481326913254726 46-47 18.22031986892097 30.107214730675373 34.32697582575569 17.345489574647964 48-49 17.74005084259894 30.390530529569176 34.006699465527 17.86271916230488 50-51 17.721934346984003 30.517054970831715 33.41496669911629 18.34604398306799 52-53 18.0632374832477 31.29853802063098 32.78714645549672 17.851078040624603 54-55 18.431824498449547 30.660822823583167 32.530969021520065 18.376383656447217 56-57 18.556457257439043 31.413130312171226 31.91391681545475 18.116495614934976 58-59 18.58883412711232 30.768357685104746 32.389602150115174 18.25320603766776 60-61 18.71055660568174 30.993031333534145 32.51794551664025 17.77846654414386 62-63 18.511929967011973 30.67311875835796 32.92480271936603 17.890148555264044 64-65 18.660718053488043 30.727031703139758 32.43092813208017 18.181322111292033 66-67 18.539141088939626 31.203444607905194 31.521538257818833 18.735876045336347 68-69 18.58345010833519 30.599634177751195 32.2448884562272 18.572027257686415 70-71 18.408833283130996 31.033556988383616 32.69016136049789 17.8674483679875 72-73 18.649104068868976 30.174049573788814 32.93372699783766 18.243119359504554 74-75 18.02620416490231 31.357543956147893 32.25398308253992 18.36226879640988 76-77 17.74718102962811 31.060840867321772 33.03037314160406 18.16160496144606 78-79 17.560413783670125 30.83209282630428 32.52325677840688 19.084236611618714 80-81 17.529128269154373 30.891535303884204 32.476765048696265 19.102571378265154 82-83 17.45609293114199 31.359240183435112 32.30922916074 18.875437724682907 84-85 17.31838210042898 30.66077134857948 32.13069620437361 19.89015034661793 86-87 16.82971512720108 30.79498675094839 32.47654677766476 19.89875134418577 88-89 17.590680700038853 30.190958049762884 32.46294121670093 19.75542003349733 90-91 17.15591717461743 29.82624773250344 32.968763302100754 20.049071790778374 92-93 17.25519070856437 29.683206165187382 32.97839754312161 20.08320558312664 94-95 17.08254573869465 29.321802802309016 32.997705243888774 20.59794621510756 96-97 17.45731709978915 29.163847332509725 32.5615997229413 20.81723584475982 98-99 16.853373417624816 29.344669853911093 32.52902824938502 21.27292847907907 100-101 16.696415902318694 29.468988877690983 32.91926957319003 20.91532564680029 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2037.0 1 1349.5 2 827.5 3 1007.5 4 819.5 5 758.5 6 1419.5 7 2693.0 8 3901.5 9 4208.5 10 3407.0 11 2531.5 12 2341.5 13 2333.0 14 2205.0 15 2175.5 16 2253.5 17 2315.5 18 2528.5 19 2818.0 20 3106.5 21 3557.5 22 4083.5 23 4516.0 24 5007.0 25 6110.0 26 8701.5 27 11916.5 28 12771.0 29 13362.0 30 15371.0 31 17162.0 32 20372.0 33 24604.0 34 29435.0 35 33187.5 36 37829.5 37 41933.5 38 39333.5 39 34939.0 40 31439.0 41 27324.0 42 24145.5 43 23130.0 44 22407.5 45 21397.0 46 19237.5 47 16811.0 48 17705.5 49 15834.5 50 11148.0 51 8182.0 52 6084.0 53 4986.5 54 4118.0 55 3468.0 56 3124.0 57 2817.5 58 2575.0 59 2449.5 60 2553.5 61 2494.5 62 1700.0 63 780.0 64 281.5 65 173.0 66 126.0 67 97.0 68 76.0 69 46.5 70 27.0 71 20.5 72 24.5 73 31.0 74 35.5 75 36.5 76 28.0 77 19.5 78 24.0 79 24.5 80 12.0 81 5.0 82 4.0 83 2.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.058496636443404505 2 0.0 3 0.0 4 0.0 5 0.017170654478412265 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.002764766399066382 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.637850525087345E-4 24-25 0.0 26-27 5.092990735122283E-4 28-29 0.0022554673255541537 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01360556096382667 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.455140210034938E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.27570105017469E-5 84-85 0.0010185981470244565 86-87 0.0 88-89 0.0 90-91 0.009094626312718363 92-93 8.003271155192159E-4 94-95 0.0 96-97 0.0 98-99 7.27570105017469E-5 100-101 0.012586962816802213 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 687219.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.27555036183121 #Duplication Level Percentage of deduplicated Percentage of total 1 75.81386241854669 24.46934134614746 2 11.925376498204333 7.697961795031843 3 4.104807473388257 3.9745476099889134 4 1.9426433488752832 2.5079953296680255 5 1.0908677178839536 1.7604177983329712 6 0.7089915805787425 1.3729856079050105 7 0.5202161987085848 1.1753184884321521 8 0.35871989633032736 0.926230566384028 9 0.29749230211553207 0.8641555001268265 >10 2.4768052707328096 17.01269260853986 >50 0.42249404806402385 9.60168945957732 >100 0.3155105766627168 19.283118663857447 >500 0.01677283238005822 3.5765836013353716 >1k 0.004079878146500649 2.051871893030364 >5k 9.066395881112552E-4 2.1425849972573197 >10k+ 4.533197940556276E-4 1.5825047343850838 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10816 1.5738796511737887 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8637 1.256804599407176 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6007 0.8741027241679873 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2859 0.41602458604898873 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2104 0.30616150019135097 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1804 0.2625072938903028 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1581 0.23005766720652368 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1236 0.17985532996031833 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1161 0.1689417783850563 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1134 0.16501289981796197 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1104 0.16064747918785716 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1041 0.15148009586463704 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 973 0.14158514243639947 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 965 0.1404210302683715 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 894 0.13008953477712346 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 877 0.12761579642006404 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 873 0.12703374033605008 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 839 0.1220862636219313 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 832 0.12106766547490683 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 823 0.11975803928587539 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 791 0.11510159061376359 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 775 0.11277336627770768 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 757 0.1101541138996448 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 720 0.10477009512251552 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.455140210034938E-4 0.0 10-11 0.0 0.0 0.0 1.455140210034938E-4 0.0 12-13 0.0 0.0 0.0 1.455140210034938E-4 0.0 14-15 0.0 0.0 0.0 1.455140210034938E-4 0.0 16-17 0.0 0.0 0.0 2.910280420069876E-4 0.0 18-19 0.0 0.0 0.0 2.910280420069876E-4 0.0 20-21 0.0 0.0 0.0 3.637850525087345E-4 0.0 22-23 0.0 0.0 0.0 6.54813094515722E-4 0.0 24-25 0.0 0.0 0.0 0.00210995330455066 0.0 26-27 0.0 0.0 0.0 0.0029830374305716226 0.0 28-29 0.0 0.0 0.0 0.005311261766627523 0.0 30-31 0.0 0.0 0.0 0.010258738480746312 0.0 32-33 0.0 0.0 0.0 0.015570000247373834 0.0 34-35 0.0 0.0 0.0 0.020735747992997863 0.0 36-37 0.0 0.0 0.0 0.02837523409568129 0.0 38-39 0.0 0.0 0.0 0.04103495392298525 0.0 40-41 0.0 0.0 0.0 0.0539857017922962 0.0 42-43 0.0 0.0 0.0 0.06882813193465256 0.0 44-45 0.0 0.0 0.0 0.07574004793231852 0.0 46-47 0.0 0.0 0.0 0.08745392662309977 0.0 48-49 0.0 0.0 0.0 0.10244187078645964 0.0 50-51 0.0 0.0 0.0 0.12143145052741558 0.0 52-53 0.0 0.0 0.0 0.17461682520419253 0.0 54-55 0.0 0.0 0.0 0.20939467622402758 0.0 56-57 0.0 0.0 0.0 0.30426981791830554 0.0 58-59 0.0 0.0 0.0 0.44432706313416825 0.0 60-61 0.0 0.0 0.0 0.5758717381213267 0.0 62-63 0.0 0.0 0.0 0.6358962717852679 0.0 64-65 0.0 0.0 0.0 0.739065712676745 0.0 66-67 0.0 0.0 0.0 0.9779997351644818 0.0 68-69 0.0 0.0 0.0 1.311372357283486 0.0 70-71 0.0 0.0 0.0 1.6133867078762374 0.0 72-73 0.0 0.0 0.0 1.952434376814378 0.0 74-75 0.0 0.0 0.0 2.376753262060566 0.0 76-77 0.0 0.0 0.0 2.657813593628814 0.0 78-79 0.0 0.0 0.0 3.0280012630617024 0.0 80-81 0.0 0.0 0.0 3.5315525327442927 0.0 82-83 0.0 0.0 0.0 3.961764735841122 0.0 84-85 0.0 0.0 0.0 4.461605397988123 0.0 86-87 0.0 0.0 0.0 5.125876903869072 0.0 88-89 0.0 0.0 0.0 6.028063834090734 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTACT 45 9.6239644E-4 42.2208 7 CTTTGCG 60 8.281813E-5 39.58776 2 TGCGTAC 50 0.001615631 37.99872 6 TATATCG 55 0.0025774934 34.549316 4 GTGCGTA 55 0.0025774934 34.549316 5 CATATAG 245 0.0 32.962868 3 GTATCAA 8545 0.0 32.485973 1 GGTATCA 5530 0.0 32.233036 1 ATGGGCG 60 0.0039505083 31.665598 7 TCCCTCG 60 0.0039505083 31.665598 9 GACATAT 260 0.0 31.079258 1 CGGATTA 65 0.0058434205 29.229784 9 GTCGGGA 200 2.3646862E-11 28.503187 2 GGACCAC 235 0.0 28.296919 6 GGTATGG 135 4.19539E-7 28.167692 1 GCAGATC 85 6.40035E-4 27.944302 3 CATTCCG 205 3.092282E-11 27.803942 9 GTATTGA 70 0.008360902 27.161705 1 GACAGTA 70 0.008390642 27.141945 7 ATCGGAT 70 0.008390642 27.141945 7 >>END_MODULE