##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937196_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 721145 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.87265945128927 33.0 31.0 34.0 30.0 34.0 2 31.95786422980122 33.0 31.0 34.0 30.0 34.0 3 31.96410707971351 33.0 31.0 34.0 30.0 34.0 4 35.621653065610936 37.0 35.0 37.0 33.0 37.0 5 35.66126091146718 37.0 35.0 37.0 35.0 37.0 6 35.72718662682262 37.0 35.0 37.0 35.0 37.0 7 35.62923822532223 37.0 35.0 37.0 33.0 37.0 8 35.65447448155364 37.0 35.0 37.0 33.0 37.0 9 37.27363983664866 39.0 37.0 39.0 34.0 39.0 10-11 37.18090883248168 39.0 37.0 39.0 34.0 39.0 12-13 37.07039222347794 39.0 37.0 39.0 33.5 39.0 14-15 38.19015177252841 40.0 38.0 41.0 33.0 41.0 16-17 38.15411533048139 40.0 38.0 41.0 33.0 41.0 18-19 38.253963488618794 40.0 38.0 41.0 33.5 41.0 20-21 38.237344778095945 40.0 38.0 41.0 34.0 41.0 22-23 38.101051106226905 40.0 38.0 41.0 33.5 41.0 24-25 38.02843394878977 40.0 38.0 41.0 33.5 41.0 26-27 38.023195751201214 40.0 38.0 41.0 33.5 41.0 28-29 37.933303288520335 40.0 37.5 41.0 33.0 41.0 30-31 37.637428672458384 39.5 37.0 41.0 33.0 41.0 32-33 37.31283445076926 39.0 35.5 41.0 33.0 41.0 34-35 37.22179034729493 39.0 35.0 41.0 33.0 41.0 36-37 37.029573109430146 39.0 35.0 41.0 32.5 41.0 38-39 36.85648031949192 39.0 35.0 41.0 32.0 41.0 40-41 36.61151848796012 39.0 35.0 41.0 31.0 41.0 42-43 36.23806654694964 39.0 35.0 41.0 30.0 41.0 44-45 35.813669927684444 39.0 35.0 41.0 29.5 41.0 46-47 35.23623057776175 39.0 35.0 41.0 25.5 41.0 48-49 34.74913921610772 39.0 35.0 41.0 23.0 41.0 50-51 33.74243876058213 38.0 34.0 39.5 20.0 40.5 52-53 33.83861151363457 38.0 34.5 39.5 19.5 40.5 54-55 34.21421212100202 39.0 35.0 40.0 17.5 41.0 56-57 34.04160952374349 38.5 34.5 40.0 13.5 41.0 58-59 33.83653079477775 38.0 34.0 40.0 8.5 41.0 60-61 33.57120828682165 38.0 34.0 40.0 7.5 41.0 62-63 33.29923871066151 38.0 34.0 40.0 2.0 41.0 64-65 32.931415318694576 37.5 33.0 40.0 2.0 41.0 66-67 32.581462119268664 37.0 33.0 39.5 2.0 41.0 68-69 32.11459207232942 36.5 32.0 39.0 2.0 41.0 70-71 31.691082930617284 36.0 32.0 39.0 2.0 40.0 72-73 31.238282176261364 35.5 31.0 38.5 2.0 40.0 74-75 30.740328228026264 35.0 31.0 37.5 2.0 39.5 76-77 30.256860964161163 35.0 30.5 37.0 2.0 39.0 78-79 29.76477892795485 35.0 30.0 36.5 2.0 39.0 80-81 29.253182785708837 34.5 29.0 36.0 2.0 38.5 82-83 28.75849447753226 34.0 29.0 35.5 2.0 37.0 84-85 28.22707985217952 34.0 28.5 35.0 2.0 37.0 86-87 27.791182078500164 34.0 27.0 35.0 2.0 36.0 88-89 27.331375104867952 34.0 26.0 35.0 2.0 36.0 90-91 26.83474127949303 33.5 25.5 35.0 2.0 35.5 92-93 26.372827240014146 33.0 25.0 35.0 2.0 35.0 94-95 25.8915391495469 33.0 23.5 35.0 2.0 35.0 96-97 25.23246087818677 32.5 19.0 35.0 2.0 35.0 98-99 24.441875767009407 32.0 2.0 34.5 2.0 35.0 100-101 21.97845925576687 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.05676722913792176 1101 2 0.001488605479629257 1101 3 -0.027389700584119936 1101 4 0.05269040712975226 1101 5 -0.002621274856402067 1101 6 -0.013783756456589913 1101 7 0.08056364187515896 1101 8 -0.15109185263478508 1101 9 -0.1174057397095396 1101 10-11 -0.21746941226595595 1101 12-13 -0.04186533625355082 1101 14-15 -0.053141988064759005 1101 16-17 -0.1537243065863123 1101 18-19 -0.10760443146871523 1101 20-21 -0.041328695355907996 1101 22-23 -0.09839021586295615 1101 24-25 -0.10690493850175642 1101 26-27 -0.13956877707142468 1101 28-29 -0.16103529036955422 1101 30-31 -0.07408112758503904 1101 32-33 -0.41543137862235113 1101 34-35 -0.10970241036970663 1101 36-37 0.26242182570150874 1101 38-39 0.12504549075818971 1101 40-41 0.049175803149601904 1101 42-43 0.017435710657544234 1101 44-45 0.02871041673218855 1101 46-47 0.2774020531662771 1101 48-49 0.3884550407639935 1101 50-51 0.6002665184134415 1101 52-53 0.44026680815750296 1101 54-55 0.23687367819601235 1101 56-57 0.19498210870084876 1101 58-59 0.17057766761034543 1101 60-61 0.2897511094965921 1101 62-63 0.126631373383141 1101 64-65 0.26045799107463097 1101 66-67 0.18239731789496005 1101 68-69 0.4390468080463066 1101 70-71 0.33197806980080813 1101 72-73 0.4271733551686694 1101 74-75 0.3801799475153338 1101 76-77 0.5070213281457576 1101 78-79 0.2405763027451009 1101 80-81 0.49332796727067674 1101 82-83 0.3983036968578233 1101 84-85 0.3242363752248387 1101 86-87 0.4294525348754341 1101 88-89 0.41159784636191077 1101 90-91 0.1864147901771993 1101 92-93 0.19781520214572978 1101 94-95 0.19362234778249032 1101 96-97 0.20047670997250577 1101 98-99 0.39520888705476764 1101 100-101 1.10927801203011 1102 1 -0.23889742233134115 1102 2 -0.4342993259713879 1102 3 -0.12504005088844394 1102 4 -0.28082580662213275 1102 5 -0.3397145672494588 1102 6 -0.17984748924335747 1102 7 -0.1848166273019558 1102 8 -0.07037768381849219 1102 9 -0.4408531395084765 1102 10-11 -0.09923254575568308 1102 12-13 -0.12863715916088836 1102 14-15 -0.058333817674245836 1102 16-17 -0.04761911397739027 1102 18-19 -0.24274085142865687 1102 20-21 -0.29201410726548005 1102 22-23 -0.24529202934051142 1102 24-25 -0.08705176238323986 1102 26-27 -0.2344852729371354 1102 28-29 -0.24349981182812996 1102 30-31 -0.16625831377236722 1102 32-33 -0.2810992168666928 1102 34-35 -0.06387527912442437 1102 36-37 -0.4047641278354277 1102 38-39 0.009951621668363941 1102 40-41 -0.010377490646227727 1102 42-43 -0.14017162118468462 1102 44-45 -0.09209742634394757 1102 46-47 -0.27623854005207704 1102 48-49 -0.47624566140329705 1102 50-51 -0.5944481335812313 1102 52-53 -0.5099307494537229 1102 54-55 -0.21629932304310984 1102 56-57 -0.414574692034833 1102 58-59 -0.6223180515850828 1102 60-61 -0.326411986585768 1102 62-63 -0.14986979170021186 1102 64-65 -0.36469923672838434 1102 66-67 -0.4644889091832951 1102 68-69 -0.49693673271601924 1102 70-71 -0.6172648208528067 1102 72-73 -0.2631802222996278 1102 74-75 -0.15254447715170016 1102 76-77 -0.2321246930361589 1102 78-79 -0.664161962644517 1102 80-81 -0.4995685312447229 1102 82-83 -0.48117291185341315 1102 84-85 -0.1636903998712107 1102 86-87 -0.2546236202715839 1102 88-89 -0.21807554064059076 1102 90-91 -0.378287470630017 1102 92-93 -0.3064352089455511 1102 94-95 -0.2516218521286646 1102 96-97 -0.06689238375630424 1102 98-99 -0.040287578729191864 1102 100-101 0.14336858459648738 1103 1 0.04346541410757965 1103 2 0.018817586554490617 1103 3 -0.03574786662343499 1103 4 0.06553806501347736 1103 5 0.13595992809215574 1103 6 -0.10024895145868129 1103 7 -0.03608636073880689 1103 8 0.041083884504487855 1103 9 -0.027581594477418037 1103 10-11 0.022563196997950286 1103 12-13 -0.004399807970408176 1103 14-15 -0.12309588418563067 1103 16-17 -0.05826950864612712 1103 18-19 -0.18226918993797625 1103 20-21 -0.024360768756160667 1103 22-23 -0.19874457204237927 1103 24-25 -0.15735617490911125 1103 26-27 -0.19965575689158754 1103 28-29 -0.0781683871386889 1103 30-31 -0.40865527935207524 1103 32-33 -0.13653885195986248 1103 34-35 0.07196517118612888 1103 36-37 0.04285087604655757 1103 38-39 -0.24533758640617265 1103 40-41 0.06118290661050452 1103 42-43 0.08853648569937178 1103 44-45 0.015650373863103084 1103 46-47 0.14348923556904225 1103 48-49 -0.035464573152772516 1103 50-51 -0.013728671883512789 1103 52-53 0.030413615194312626 1103 54-55 0.13634042850969053 1103 56-57 0.34270403467324684 1103 58-59 0.28127925690352384 1103 60-61 0.3371676912092596 1103 62-63 0.18521205353788872 1103 64-65 0.10596506839583242 1103 66-67 0.17605925981256831 1103 68-69 0.41455718550344045 1103 70-71 0.3489174061140723 1103 72-73 0.5124279557542017 1103 74-75 0.4023888820615511 1103 76-77 0.01196336338205839 1103 78-79 0.37967376458115254 1103 80-81 0.2725709291590057 1103 82-83 0.2974543202894431 1103 84-85 0.32621400618468854 1103 86-87 0.5259300482066784 1103 88-89 0.7447236959018504 1103 90-91 0.2480154214507273 1103 92-93 0.3436852944188402 1103 94-95 0.27373723172226505 1103 96-97 0.5692481118441073 1103 98-99 0.6801478508774359 1103 100-101 1.1229752098552908 1104 1 0.13532147471363842 1104 2 0.1692673971605494 1104 3 0.17810945660888677 1104 4 0.15423755996297217 1104 5 0.17206314778912457 1104 6 0.15231575813727716 1104 7 0.1658154700692691 1104 8 0.07683199056509693 1104 9 0.07686127168420143 1104 10-11 0.11256477528077369 1104 12-13 0.1269725089487821 1104 14-15 0.18200591505679142 1104 16-17 0.0938177703942813 1104 18-19 0.14909128986000297 1104 20-21 0.266911327203438 1104 22-23 0.20168551002832658 1104 24-25 0.1603916154949232 1104 26-27 0.19961034159326374 1104 28-29 0.18364900553807217 1104 30-31 0.14048850221357867 1104 32-33 0.18823229703003364 1104 34-35 0.17842034795380357 1104 36-37 0.273931210642516 1104 38-39 0.31771481258372347 1104 40-41 0.3874336957519162 1104 42-43 0.4965817824165413 1104 44-45 0.23416363143885377 1104 46-47 0.33246887572055783 1104 48-49 0.21759334982701972 1104 50-51 0.11190264124780214 1104 52-53 0.08484464423471394 1104 54-55 0.16790608507534444 1104 56-57 0.13484418618840266 1104 58-59 0.4214307720550394 1104 60-61 0.37609209146178557 1104 62-63 0.6755450712146498 1104 64-65 0.7782543676382545 1104 66-67 0.5629555666307411 1104 68-69 0.5593646349983956 1104 70-71 0.5189576523261898 1104 72-73 0.6459506830269284 1104 74-75 0.6668301699403365 1104 76-77 0.5818418356042834 1104 78-79 0.5737630953892356 1104 80-81 0.5913248748660784 1104 82-83 0.5707931776126749 1104 84-85 0.7569076614877197 1104 86-87 0.6321682110854674 1104 88-89 0.42255671357861857 1104 90-91 0.8133760590769903 1104 92-93 0.804839808307726 1104 94-95 0.8277934185909501 1104 96-97 0.7352242797734014 1104 98-99 1.0670638862309687 1104 100-101 1.543429755309834 1105 1 -0.0010372740644619682 1105 2 -0.0404784483917453 1105 3 -0.0739400709245075 1105 4 -0.05431408552415462 1105 5 -0.16295640255300725 1105 6 0.006375712519812282 1105 7 -0.05171304084633732 1105 8 -0.10971248646325193 1105 9 -0.21833260254193476 1105 10-11 -0.13682020353968483 1105 12-13 -0.24513485501341847 1105 14-15 -0.17927951993831925 1105 16-17 -0.07213123579666103 1105 18-19 -0.06562604477668543 1105 20-21 -0.16203213972389818 1105 22-23 -0.25619164865528177 1105 24-25 -0.13571134964030307 1105 26-27 -0.0823153671066379 1105 28-29 -0.3216084072999763 1105 30-31 -0.06255564897573862 1105 32-33 -0.2518866342179322 1105 34-35 -0.3315731352654794 1105 36-37 -0.3251808779857015 1105 38-39 -0.17442024769649578 1105 40-41 -0.3992614818303579 1105 42-43 -0.27672141080601165 1105 44-45 -0.23185298635196006 1105 46-47 -0.2645374781406318 1105 48-49 -0.3160853728839612 1105 50-51 -0.49902437080824313 1105 52-53 -0.45699145873042113 1105 54-55 -0.3857563456838591 1105 56-57 -0.4701387072622367 1105 58-59 -0.2006831086878691 1105 60-61 -0.10020882088751648 1105 62-63 -0.041298363128781546 1105 64-65 0.24215710468615725 1105 66-67 0.15487394395234588 1105 68-69 -0.04768407793741858 1105 70-71 0.06473477842589759 1105 72-73 0.02252204177570505 1105 74-75 0.006397618620692214 1105 76-77 0.14319488219926768 1105 78-79 0.09411092855964753 1105 80-81 0.17513393319126536 1105 82-83 0.25274413883783 1105 84-85 0.4200723731201741 1105 86-87 0.38057974578732257 1105 88-89 0.19055198891260616 1105 90-91 0.38736269967653314 1105 92-93 0.513504682859697 1105 94-95 0.6215698930125875 1105 96-97 0.6880745567365807 1105 98-99 0.8114415336731327 1105 100-101 1.2409321991026019 1106 1 -0.1899839669711092 1106 2 -0.2465552859035114 1106 3 -0.25697078881618296 1106 4 -0.009036426586874313 1106 5 -0.13815106328715387 1106 6 -0.19742166918635462 1106 7 -0.5503816469899121 1106 8 -0.4101967852567867 1106 9 -0.5996451720636244 1106 10-11 -0.7791022263000187 1106 12-13 -0.2601831993276136 1106 14-15 -0.2522939804499984 1106 16-17 -0.3792434117186261 1106 18-19 -0.6090554963040304 1106 20-21 -0.48803367989674484 1106 22-23 -0.38635336680365384 1106 24-25 -0.3769178183662376 1106 26-27 -0.5622255236908771 1106 28-29 -0.4218924836241058 1106 30-31 -0.6188070394095533 1106 32-33 -0.39278677967869413 1106 34-35 -0.37168950388018374 1106 36-37 -0.6687549346298312 1106 38-39 -0.5429793304380013 1106 40-41 -0.6341900349235416 1106 42-43 -0.4928299074261844 1106 44-45 -0.503407705270412 1106 46-47 -0.4447847614468756 1106 48-49 -0.3226499317955742 1106 50-51 -0.34236483457139144 1106 52-53 -0.319748368116926 1106 54-55 -0.4303041338245279 1106 56-57 -0.5088791881384225 1106 58-59 -0.3406813973847136 1106 60-61 -0.4805596380347765 1106 62-63 -0.382969450661939 1106 64-65 -0.22241378604714157 1106 66-67 -0.20413007761007407 1106 68-69 -0.15643225967781405 1106 70-71 -0.29765676773650185 1106 72-73 -0.26959569588152377 1106 74-75 -0.1257457885838953 1106 76-77 -0.031019201825721154 1106 78-79 -0.049872129539092924 1106 80-81 -0.351398110960357 1106 82-83 -0.20944119529411154 1106 84-85 -0.4124868807516542 1106 86-87 -0.4279254309047289 1106 88-89 -0.2997509117462478 1106 90-91 -0.12730060142725463 1106 92-93 -0.06841026537338024 1106 94-95 0.07129608450740932 1106 96-97 0.08028128215799057 1106 98-99 0.11913298571386122 1106 100-101 0.5515782160445006 1107 1 0.06493089343915415 1107 2 -0.026123184113934883 1107 3 -0.04441926064278334 1107 4 -0.20889575204896715 1107 5 -0.08028553694742158 1107 6 -0.03133473092218253 1107 7 0.07060545434474363 1107 8 0.06740726840950373 1107 9 -0.0703029829998485 1107 10-11 0.004983354209905144 1107 12-13 0.1358558676012649 1107 14-15 0.19322962977654612 1107 16-17 -8.009098114740709E-4 1107 18-19 0.12163499871638095 1107 20-21 0.023029567655449057 1107 22-23 0.15061744148004408 1107 24-25 0.21519425934769743 1107 26-27 0.22931370770823634 1107 28-29 0.19334690804600996 1107 30-31 0.43373595030931256 1107 32-33 0.4214983347156007 1107 34-35 0.2556104548272842 1107 36-37 0.11083920883019971 1107 38-39 0.4795758370766734 1107 40-41 0.44168498169845094 1107 42-43 0.25433910498257006 1107 44-45 0.31091065310672406 1107 46-47 0.6024426903843434 1107 48-49 0.5966144313547019 1107 50-51 0.5414054530065542 1107 52-53 0.45510063421365743 1107 54-55 0.1993351514648367 1107 56-57 0.5257103011640112 1107 58-59 0.4639419657865531 1107 60-61 0.3651865319084635 1107 62-63 0.4742161487445671 1107 64-65 0.6490317323140431 1107 66-67 0.4178018527105465 1107 68-69 0.3022069766204183 1107 70-71 0.6250982136118743 1107 72-73 0.6593881580409189 1107 74-75 0.6283467206719315 1107 76-77 0.49555848761249166 1107 78-79 0.6252659204738542 1107 80-81 0.6934410231563675 1107 82-83 0.9791361722023701 1107 84-85 0.7917431536121278 1107 86-87 0.8221917978768651 1107 88-89 0.6605417752774017 1107 90-91 0.7733751262660284 1107 92-93 0.8529012272464556 1107 94-95 0.8625606156272809 1107 96-97 1.0022664028511414 1107 98-99 1.4506857246681122 1107 100-101 1.5376629847013774 1108 1 0.03627872160112133 1108 2 0.13385513500410084 1108 3 0.048675582988970234 1108 4 -0.2228178781389758 1108 5 -0.0408508761714117 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-1.4530963573954274 2316 92-93 -1.454882413914497 2316 94-95 -1.4936906528931218 2316 96-97 -1.1977691157199928 2316 98-99 -1.0371999055328232 2316 100-101 -1.3776658157857362 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2481.0 3 415.0 4 259.0 5 271.0 6 339.0 7 406.0 8 533.0 9 701.0 10 1052.0 11 1665.0 12 2835.0 13 4335.0 14 4976.0 15 7471.0 16 10438.0 17 14377.0 18 10966.0 19 5665.0 20 4970.0 21 4764.0 22 5234.0 23 5535.0 24 6407.0 25 7097.0 26 7896.0 27 9433.0 28 11019.0 29 13396.0 30 16241.0 31 19488.0 32 24845.0 33 31724.0 34 43401.0 35 63562.0 36 88363.0 37 140270.0 38 131908.0 39 16406.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.2115036263365 23.38719431241407 15.727748807556013 19.673553253693413 2 14.176950242733627 23.421953460187655 41.28653686590791 21.114559431170807 3 16.99810171790361 29.822067320920848 32.85153655235284 20.328294408822703 4 10.527961532047362 19.969008838734783 41.69478570237367 27.808243926844185 5 9.853517525085472 41.43980876671207 35.79064402256997 12.916029685632488 6 24.399700284562847 42.41168056117406 19.35472211680283 13.833897037460261 7 23.57097776010721 37.85647359099617 22.791094033680967 15.781454615215656 8 24.399034786065556 35.5737768721175 21.421105158834962 18.60608318298199 9 26.36782245028722 17.146617779711264 23.386793016652234 33.09876675334928 10-11 21.95325536769576 29.735085023411234 29.227870526167276 19.083789082725726 12-13 23.769996740332374 29.251103526070736 29.179585445340795 17.79931428825609 14-15 21.286037821862127 29.681676765051506 29.36368014376561 19.668605269320764 16-17 17.27298380034418 32.95936324538016 30.310203809296105 19.457449144979552 18-19 16.998559385226507 30.660354740095165 35.23898013091317 17.102105743765154 20-21 18.08450167885971 29.555783171377108 35.36313679927064 16.996578350492538 22-23 18.21974568986783 29.579552348015337 34.751641467046625 17.449060495070214 24-25 17.697546386981696 30.466326139931887 34.97109043368171 16.8650370394047 26-27 17.280730038191916 30.326438034033607 35.54627781700124 16.846554110773234 28-29 16.719974534546555 30.330022268371405 35.93395106518433 17.016052131897705 30-31 17.544754651307453 29.999047810346063 35.49728278434156 16.95891475400493 32-33 16.9453912105509 30.432939305651313 35.356779463114115 17.264890020683673 34-35 17.489288881845454 30.588548487098006 34.86840824860359 17.05375438245295 36-37 17.963403185491913 30.03063748056928 34.35004277017303 17.65591656376578 38-39 16.785400096450644 31.49669441128278 34.3319125162083 17.385992976058283 40-41 16.964994225694063 30.973071305382266 34.958801612636485 17.103132856287186 42-43 16.92850571070399 30.78478053607514 34.24949202669535 18.03722172652552 44-45 17.561144113769164 30.989739173587495 33.42375760457001 18.02535910807334 46-47 18.12190909122503 31.38038291646249 32.61817132887791 17.87953666343457 48-49 17.63893841479563 31.51571517842186 32.50157614026015 18.343770266522363 50-51 17.628998265008786 31.53826156104722 32.13132826923505 18.701411904708944 52-53 17.87042212785365 32.3814668657862 31.655348000322597 18.09276300603755 54-55 18.240876155946417 31.760861586711968 31.39843797787923 18.599824279462386 56-57 18.303420356481986 32.51016767897582 30.78161013786907 18.404801826673125 58-59 18.311252595809606 31.581649632227016 31.434151370872325 18.672946401091057 60-61 18.50282976526774 31.926046763034776 31.59137995000493 17.97974352169255 62-63 18.253496779232 31.489599118431578 31.928715799098562 18.328188303237862 64-65 18.365289621373915 31.59531792558092 31.593233668806043 18.446158784239127 66-67 18.21476831797355 31.85321304880972 30.8845914691548 19.04742716406193 68-69 18.109541986323926 31.382989810338625 31.58968722339023 18.917780979947224 70-71 18.193882336593077 31.86048531378646 31.75821159959007 18.187420750030398 72-73 18.30274387956167 31.136140670726114 31.881862825476144 18.679252624236074 74-75 17.57099659260824 32.20151970784256 31.528379182114808 18.699104517434396 76-77 17.504709714137203 31.89694290735466 31.991936330085117 18.60641104842302 78-79 17.436989971760834 31.63856647321726 31.645654059102128 19.278789495919774 80-81 17.26336926727417 31.6783107457885 31.596460570519348 19.46185941641798 82-83 17.40387401226861 32.0763880348757 31.36304673924447 19.15669121361122 84-85 17.423993452149762 31.380697321037122 31.252575124144432 19.94273410266868 86-87 17.042638037022957 31.650593644491476 31.56519666666898 19.741571651816578 88-89 17.740766603784728 31.04318008900747 31.527961617698658 19.68809168950914 90-91 17.36566600490174 30.69938384288306 32.18588807718259 19.749062075032608 92-93 17.653733139433488 30.56694859963411 32.2567398840075 19.522578376924898 94-95 17.422963348966476 30.08956053500087 32.369636963696365 20.117839152336288 96-97 17.806759955028134 29.88648634375682 31.855464651363818 20.45128904985123 98-99 17.60333174427348 30.025656599069077 31.71487818674835 20.65613346990909 100-101 17.647986527289326 29.625859934387233 32.43114619475126 20.295007343572184 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2205.0 1 1383.0 2 643.0 3 768.5 4 698.0 5 739.5 6 1318.5 7 2554.5 8 3886.0 9 4176.5 10 3354.0 11 2629.5 12 2514.0 13 2512.0 14 2464.0 15 2326.0 16 2267.5 17 2364.5 18 2478.5 19 2721.5 20 2990.0 21 3364.0 22 3796.5 23 4179.0 24 4647.5 25 5832.5 26 8656.5 27 12133.0 28 13067.5 29 13819.0 30 16279.0 31 18321.0 32 21553.0 33 26233.5 34 31723.5 35 35624.0 36 40398.0 37 44873.5 38 41781.5 39 36613.5 40 32748.0 41 28939.5 42 25992.5 43 24359.0 44 23360.5 45 22135.5 46 19958.5 47 17430.5 48 18049.5 49 16486.0 50 11892.5 51 8667.0 52 6511.0 53 5366.0 54 4451.5 55 3877.5 56 3455.0 57 3079.0 58 2874.5 59 2594.5 60 2693.5 61 2823.5 62 2164.0 63 1079.0 64 363.5 65 217.0 66 160.5 67 123.5 68 95.5 69 71.0 70 46.5 71 35.0 72 31.0 73 32.0 74 38.0 75 33.0 76 18.5 77 14.5 78 17.5 79 20.0 80 17.0 81 12.5 82 8.5 83 5.0 84 4.0 85 2.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2529311026215255 2 0.22630677602978594 3 0.2145199647782346 4 0.2197893627495164 5 0.22256273010282257 6 0.24904838832689682 7 0.2505737403712152 8 0.23837092401666793 9 0.23046682705974525 10-11 0.23927226840649243 12-13 0.24218430412746395 14-15 0.2274854571549411 16-17 0.24225363831129662 18-19 0.2302588245082473 20-21 0.22311740357348386 22-23 0.22055203877167562 24-25 0.21819467652136534 26-27 0.22478142398546755 28-29 0.24114429136997415 30-31 0.2427389775981252 32-33 0.2408669546346435 34-35 0.2494643934298927 36-37 0.222077390815994 38-39 0.22270139847048792 40-41 0.2188186841758592 42-43 0.22450408725013696 44-45 0.2438483245394477 46-47 0.24925639087839477 48-49 0.2530004368053581 50-51 0.2541791179305133 52-53 0.27442469960964855 54-55 0.2525150975185296 56-57 0.21985869693334903 58-59 0.20460517649016496 60-61 0.204397173938667 62-63 0.21056791629977326 64-65 0.20301049026201387 66-67 0.2028024877105159 68-69 0.20717054129197318 70-71 0.20904256425545487 72-73 0.20897323007162222 74-75 0.21257860763092026 76-77 0.22485075816930022 78-79 0.2186800158081939 80-81 0.21320261528541418 82-83 0.20848789078479363 84-85 0.2107065846674386 86-87 0.21694666121237755 88-89 0.21389595712374074 90-91 0.22249339591898995 92-93 0.2120932683440917 94-95 0.21077591885127123 96-97 0.21965069438185109 98-99 0.22769345970643906 100-101 0.2035651637326751 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 721145.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.6162523414914 #Duplication Level Percentage of deduplicated Percentage of total 1 76.61931520956661 25.75654234317061 2 11.772360420456558 7.914852770981071 3 3.9716009244576624 4.005310166288079 4 1.806312846201029 2.4288587378228534 5 1.011323628867597 1.699845515346297 6 0.6762074914580034 1.363893700083548 7 0.47511957829201484 1.1180217745373147 8 0.3499364638991931 0.9410841979139578 9 0.27106699419983954 0.8201030830624253 >10 2.3147102534651323 16.537873998474847 >50 0.4054536704948477 9.647340074830606 >100 0.3067740092928621 19.66748800016047 >500 0.014863882011498028 3.426195774763311 >1k 0.0041288561143050075 2.4317176412336727 >5k 8.257712228610015E-4 2.2408722213309162 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9236 1.2807410437568034 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6909 0.958059752199627 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4927 0.6832190474869825 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2875 0.39867155703776636 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1527 0.21174659742492838 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1440 0.19968244943804644 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1236 0.17139410243432318 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1158 0.16057796975642902 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1131 0.15683392382946562 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1116 0.154753898314486 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 1056 0.14643379625456737 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1054 0.14615645951923675 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 997 0.1382523625623141 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 935 0.12965492376706486 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 932 0.12923891866406895 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 913 0.12660421967842805 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 892 0.12369218395745654 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 865 0.11994813803049317 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 862 0.11953213292749723 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 797 0.11051868902925209 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 788 0.1092706737202643 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 744 0.10316926554299066 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 742 0.10289192880766004 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 732 0.10150524513100694 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 725 0.10053456655734977 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.933418383265501E-5 0.0 12-13 0.0 0.0 0.0 2.7733673533062006E-4 0.0 14-15 0.0 0.0 0.0 2.7733673533062006E-4 0.0 16-17 0.0 0.0 0.0 2.7733673533062006E-4 0.0 18-19 0.0 0.0 0.0 4.160051029959301E-4 0.0 20-21 0.0 0.0 0.0 5.546734706612401E-4 0.0 22-23 0.0 0.0 0.0 0.0012480153089877903 0.0 24-25 0.0 0.0 0.0 0.0015253520443184103 0.0 26-27 0.0 0.0 0.0 0.0027040331694735457 0.0 28-29 0.0 0.0 0.0 0.005546734706612402 0.0 30-31 0.0 0.0 0.0 0.013797502582698348 0.0 32-33 0.0 0.0 0.0 0.019552239840808713 0.0 34-35 0.0 0.0 0.0 0.02502964036358846 0.0 36-37 0.0 0.0 0.0 0.03293373732051113 0.0 38-39 0.0 0.0 0.0 0.045413890410389034 0.0 40-41 0.0 0.0 0.0 0.06177675779489562 0.0 42-43 0.0 0.0 0.0 0.08021965069438186 0.0 44-45 0.0 0.0 0.0 0.08805441346747186 0.0 46-47 0.0 0.0 0.0 0.09907854869686403 0.0 48-49 0.0 0.0 0.0 0.12126548752331362 0.0 50-51 0.0 0.0 0.0 0.1504551789168614 0.0 52-53 0.0 0.0 0.0 0.20876522752012425 0.0 54-55 0.0 0.0 0.0 0.2535551102760194 0.0 56-57 0.0 0.0 0.0 0.35672437581901006 0.0 58-59 0.0 0.0 0.0 0.511894279236492 0.0 60-61 0.0 0.0 0.0 0.650562646901802 0.0 62-63 0.0 0.0 0.0 0.7227395322715959 0.0 64-65 0.0 0.0 0.0 0.8463623820452197 0.0 66-67 0.0 0.0 0.0 1.0809199259510918 0.0 68-69 0.0 0.0 0.0 1.4246094752095626 0.0 70-71 0.0 0.0 0.0 1.7550561953559964 0.0 72-73 0.0 0.0 0.0 2.10664984157139 0.0 74-75 0.0 0.0 0.0 2.5401271588931493 0.0 76-77 0.0 0.0 0.0 2.860659090751513 0.0 78-79 0.0 0.0 0.0 3.2597466528922756 0.0 80-81 0.0 0.0 0.0 3.7828037357258246 0.0 82-83 0.0 0.0 0.0 4.221273114283536 0.0 84-85 0.0 0.0 0.0 4.7291460108577335 0.0 86-87 0.0 0.0 0.0 5.410978374668062 0.0 88-89 0.0 0.0 0.0 6.302407976204508 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATCGC 25 0.0050206594 56.999584 3 CATTCCG 235 0.0 46.49872 9 CGTCACT 75 6.9157177E-6 38.002365 1 GGGCGTC 25 0.0016165614 37.997078 30-31 GGCATTC 315 0.0 34.68952 7 TTACTCG 30 0.003953439 31.663134 56-57 ATGGGTA 380 0.0 31.254118 5 TAGGCAT 320 0.0 31.175982 5 GGTATCA 5395 0.0 30.905537 1 GTATCAA 8170 0.0 30.583296 1 CATGGGT 645 0.0 30.199877 4 TTAGGCA 340 0.0 29.344141 4 GTACATG 5485 0.0 28.406507 1 GGGTATG 170 3.6288839E-9 27.9468 7 TACATGG 5750 0.0 27.012846 2 ACATGGG 5455 0.0 26.993385 3 CCTTTAG 375 0.0 26.601656 1 CATGGGG 1980 0.0 26.3942 4 TGGGTAT 255 0.0 26.083681 6 TAGGACA 330 0.0 25.914307 4 >>END_MODULE