FastQCFastQC Report
Thu 26 May 2016
SRR937196_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937196_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721145
Sequences flagged as poor quality0
Sequence length101
%GC36

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111591.5474003147771946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89491.2409432222368593No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62650.8687573234231672No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28220.39132213355150486No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21050.2918969139354776No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18880.2618058781521054No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15600.21632265355788363No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT12430.17236478100798036No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12330.17097809733132727No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC11610.16099397485942493No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA11120.15419922484382476No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10860.15059384728452668No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10210.14158040338628156No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA10120.14033238807729373No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT9710.13464698500301603No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT9610.13326030132636293No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT9240.12812957172274647No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA8810.12216683191313815No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8660.1200868063981585No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA8630.11967080129516255No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC8460.11731343904485228No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA8350.11578808700053386No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG7910.10968667882326023No Hit
GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG7900.10954801045559492No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG853.6389793E-739.116565
CATTCCG2450.036.8357169
GTACAGT550.00257934134.54456
GGTATCA60200.031.3412671
GGCATTC3200.031.1710117
GTATCAA89600.029.9153181
ATAAGGT804.4927277E-429.6866763
TTAGGCA3450.027.5354674
GTCGGGA1903.947207E-1027.4992372
TAGGCAT3350.026.939555
AGGGGTC1457.882627E-726.206175
GTACATG50950.025.4648441
GGGGTCC1501.0594504E-625.3326286
TAGGGCG751.0606818E-625.33262830-31
TAAGGCC950.001226309924.9993083
CATGGGT6050.024.3381674
ACATGGG50950.024.0523263
TACATGG53550.023.9489172
TATCAAC111750.023.9299832
CATGGGG18100.023.8805524
Produced by FastQC (version 0.11.5)