##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937196_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 721145 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19695622932975 33.0 31.0 34.0 31.0 34.0 2 32.40117313439045 34.0 31.0 34.0 31.0 34.0 3 32.42056590560844 34.0 31.0 34.0 31.0 34.0 4 36.09735906093781 37.0 35.0 37.0 35.0 37.0 5 36.04418528867287 37.0 35.0 37.0 35.0 37.0 6 36.10688973784745 37.0 35.0 37.0 35.0 37.0 7 36.05051411297312 37.0 35.0 37.0 35.0 37.0 8 36.0418972606064 37.0 35.0 37.0 35.0 37.0 9 37.72222645931124 39.0 37.0 39.0 35.0 39.0 10-11 37.66498207711348 39.0 37.0 39.0 35.0 39.0 12-13 37.590645431917295 39.0 37.0 39.0 35.0 39.0 14-15 38.70774948172698 40.0 38.0 41.0 34.5 41.0 16-17 38.70634269113701 40.0 38.0 41.0 34.5 41.0 18-19 38.83014234307941 40.0 38.0 41.0 35.0 41.0 20-21 38.83371790693966 40.0 38.0 41.0 35.0 41.0 22-23 38.69909102884995 40.0 38.0 41.0 34.5 41.0 24-25 38.60941350213896 40.0 38.0 41.0 34.0 41.0 26-27 38.502848248271846 40.0 38.0 41.0 34.0 41.0 28-29 38.375706688668714 40.0 38.0 41.0 34.0 41.0 30-31 38.2196389075706 40.0 37.0 41.0 34.0 41.0 32-33 37.96576555339079 40.0 36.5 41.0 34.0 41.0 34-35 37.77220461904333 40.0 36.0 41.0 33.0 41.0 36-37 37.528682858509725 40.0 36.0 41.0 33.0 41.0 38-39 37.339168267130745 40.0 35.0 41.0 33.0 41.0 40-41 37.09696940282467 40.0 35.0 41.0 33.0 41.0 42-43 36.74788496072219 40.0 35.0 41.0 32.0 41.0 44-45 36.230877285428036 40.0 35.0 41.0 30.5 41.0 46-47 35.55656490719619 39.0 35.0 41.0 27.5 41.0 48-49 34.97873728584404 39.0 35.0 41.0 23.5 41.0 50-51 34.67140380922006 39.0 35.0 41.0 20.5 41.0 52-53 34.43981584840774 39.0 35.0 41.0 17.5 41.0 54-55 34.2254116717165 39.0 35.0 40.5 13.5 41.0 56-57 34.00177703513163 39.0 35.0 40.0 8.5 41.0 58-59 33.790859674545345 38.0 34.0 40.0 6.0 41.0 60-61 33.51580472720465 38.0 34.0 40.0 2.0 41.0 62-63 33.19070644599907 38.0 34.0 40.0 2.0 41.0 64-65 32.85391426134828 37.5 33.0 40.0 2.0 41.0 66-67 32.46767987020641 37.0 33.0 39.5 2.0 41.0 68-69 32.03318056701495 36.0 32.0 39.0 2.0 40.5 70-71 31.661499421059567 36.0 32.0 39.0 2.0 40.0 72-73 31.196616491828966 35.5 31.0 38.5 2.0 40.0 74-75 30.709297714051957 35.0 31.0 37.5 2.0 39.5 76-77 29.387574620915352 34.0 29.5 36.0 2.0 38.5 78-79 29.910135270992658 35.0 30.5 36.0 2.0 39.0 80-81 29.66627446630012 35.0 30.5 36.0 2.0 38.5 82-83 29.287957345610106 34.0 30.0 36.0 2.0 37.0 84-85 28.88634116578497 34.0 29.5 35.0 2.0 37.0 86-87 28.4720160300633 34.0 29.0 35.0 2.0 36.5 88-89 27.982588106414106 34.0 29.0 35.0 2.0 36.0 90-91 27.499355192090356 34.0 27.0 35.0 2.0 36.0 92-93 27.198211178057118 34.0 27.0 35.0 2.0 35.0 94-95 26.788322736758904 34.0 26.0 35.0 2.0 35.0 96-97 26.354012022547476 33.0 25.0 35.0 2.0 35.0 98-99 25.74183416649911 33.0 23.5 35.0 2.0 35.0 100-101 23.506563867183438 31.0 8.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.14084013283029861 1101 2 0.010837840549321243 1101 3 0.05073268144873566 1101 4 0.06437696638926838 1101 5 -0.09937953685618339 1101 6 -0.1518535566097654 1101 7 -0.059937371381799665 1101 8 -0.05670461781516423 1101 9 -0.08294268477825995 1101 10-11 0.07648098605494624 1101 12-13 -0.147736252467503 1101 14-15 -0.24600893511256317 1101 16-17 -0.13964552286831378 1101 18-19 -0.11155737819000677 1101 20-21 -0.03892075815959828 1101 22-23 -0.10764046229477486 1101 24-25 -0.010612812800808058 1101 26-27 -0.08010012806231259 1101 28-29 -0.14598897441818082 1101 30-31 -0.009698819308638917 1101 32-33 -0.094599235389488 1101 34-35 0.037333984345885085 1101 36-37 0.08206441560572841 1101 38-39 -0.01837573825723382 1101 40-41 -0.12593113881587215 1101 42-43 0.1094055955852582 1101 44-45 0.15870425819403522 1101 46-47 0.06562082692779825 1101 48-49 0.26070886501170065 1101 50-51 0.23344800294351842 1101 52-53 0.1997786118901601 1101 54-55 0.22347218804463154 1101 56-57 0.4073475009630769 1101 58-59 0.46793976590013386 1101 60-61 0.5007850029756682 1101 62-63 0.42448186352802963 1101 64-65 0.3503390983444845 1101 66-67 0.3061729243616682 1101 68-69 0.4788625244231888 1101 70-71 0.5191435554419748 1101 72-73 0.32616955497574196 1101 74-75 0.24604123607960915 1101 76-77 0.04105660021451385 1101 78-79 -0.06512308682882306 1101 80-81 0.14654458680159266 1101 82-83 0.45855359555893926 1101 84-85 0.4260923122087341 1101 86-87 0.234069821071067 1101 88-89 0.3087488510988301 1101 90-91 0.12109244169273481 1101 92-93 0.13321417425687798 1101 94-95 0.09378211779139534 1101 96-97 0.2475686300055706 1101 98-99 0.241737084328026 1101 100-101 1.2088870262687124 1102 1 -0.15632054462297162 1102 2 -0.1870533196243116 1102 3 -0.2036077410985513 1102 4 -0.16087144184970015 1102 5 -0.11750184333155289 1102 6 -0.17879620645351224 1102 7 -0.2510671515356364 1102 8 -0.1533745836030178 1102 9 -0.1849665787283996 1102 10-11 -0.12718649370221158 1102 12-13 -0.397026681638998 1102 14-15 -0.2339707796070627 1102 16-17 -0.2877170956139352 1102 18-19 -0.21628629174004033 1102 20-21 -0.13449733803600594 1102 22-23 -0.1483895446455108 1102 24-25 -0.14695795802708034 1102 26-27 -0.28068150075650067 1102 28-29 -0.1981909429804105 1102 30-31 -0.220030264089921 1102 32-33 -0.1612599272697608 1102 34-35 -0.0019328314306719108 1102 36-37 -0.04799083817981398 1102 38-39 0.029511135656591136 1102 40-41 0.1443300113045325 1102 42-43 0.17040452791690086 1102 44-45 0.2752796145380927 1102 46-47 0.380894718929639 1102 48-49 0.31032660856867267 1102 50-51 0.5071537473109871 1102 52-53 0.4474254552546384 1102 54-55 0.5166858697859453 1102 56-57 0.4936439386915126 1102 58-59 0.4996136672538114 1102 60-61 0.41921280287747464 1102 62-63 0.43516088501387884 1102 64-65 0.29374176517740835 1102 66-67 0.3738903741150068 1102 68-69 0.39036061119267274 1102 70-71 0.5804300568408571 1102 72-73 0.49048137648029666 1102 74-75 0.4172812120765528 1102 76-77 0.5404463926816092 1102 78-79 0.5878098838609525 1102 80-81 0.192875938064482 1102 82-83 0.527266236908094 1102 84-85 0.43021881142551877 1102 86-87 0.5044112411479063 1102 88-89 0.47885725450601413 1102 90-91 0.5705239277779732 1102 92-93 0.6655692012603183 1102 94-95 0.7518375586137367 1102 96-97 0.712056577822306 1102 98-99 0.7799494801913944 1102 100-101 1.6879778715970772 1103 1 -0.030587819467690736 1103 2 0.045956514744219135 1103 3 0.07231099596149448 1103 4 0.048495470676179764 1103 5 0.017167540726418906 1103 6 0.010740219529921546 1103 7 -0.07752774936172102 1103 8 0.010150908115413415 1103 9 -0.12513868017767038 1103 10-11 -0.10440812284300449 1103 12-13 0.027830978816488994 1103 14-15 0.1235335876289625 1103 16-17 -0.16239440539654737 1103 18-19 -0.17138867916239775 1103 20-21 -0.1719391082223396 1103 22-23 0.012035209495266486 1103 24-25 -0.021358676194786597 1103 26-27 0.0456440362517867 1103 28-29 -0.18136414432616021 1103 30-31 -0.4138837837586564 1103 32-33 -0.38501364750784717 1103 34-35 -0.34683206150313595 1103 36-37 -0.2658132048982367 1103 38-39 -0.2570507173454857 1103 40-41 -0.0715279079501272 1103 42-43 0.05276170312394157 1103 44-45 -0.09722863468550713 1103 46-47 0.08172287627954233 1103 48-49 -0.032964022901303736 1103 50-51 -0.051734386746993266 1103 52-53 0.006612017107642032 1103 54-55 -0.15210942006913086 1103 56-57 -0.023005744279508633 1103 58-59 0.0867667146140576 1103 60-61 0.2606813264281911 1103 62-63 0.31481360887516985 1103 64-65 -0.0023196349261098703 1103 66-67 0.15865276412535678 1103 68-69 0.11893042226927619 1103 70-71 0.10797275870421075 1103 72-73 0.3483278438716013 1103 74-75 0.1163867374388694 1103 76-77 0.3676753005491058 1103 78-79 0.46438208882989684 1103 80-81 0.22595451596303207 1103 82-83 0.2731537563180346 1103 84-85 0.26319629589756843 1103 86-87 0.03222729963652071 1103 88-89 0.0880163189470089 1103 90-91 0.1804221873535461 1103 92-93 0.30820959981104323 1103 94-95 0.42324105887253793 1103 96-97 0.5433090040645432 1103 98-99 0.6083437930795945 1103 100-101 0.8718304207689229 1104 1 0.04260504669392873 1104 2 0.20788833292603925 1104 3 0.08904079394128672 1104 4 0.09067507926203433 1104 5 -0.03278511078872981 1104 6 0.15850694932790077 1104 7 0.04727496528474262 1104 8 0.10224371114571085 1104 9 0.053680764266772485 1104 10-11 0.090016593066089 1104 12-13 0.11021734245284875 1104 14-15 0.26331026060875473 1104 16-17 0.0768140857650721 1104 18-19 -0.007227536485629571 1104 20-21 0.05328185137361885 1104 22-23 -0.10700677282795823 1104 24-25 0.054379171027434836 1104 26-27 -0.0344340887482133 1104 28-29 -0.055377717558492634 1104 30-31 0.022669246544374744 1104 32-33 0.09413329062346065 1104 34-35 0.10441872763828286 1104 36-37 0.07606258424317502 1104 38-39 0.07991266649290196 1104 40-41 -0.012342301889518126 1104 42-43 0.15272619176030844 1104 44-45 0.21927625799126105 1104 46-47 0.15748045203711314 1104 48-49 0.39617107810879304 1104 50-51 0.29655317638432876 1104 52-53 0.27896444766319917 1104 54-55 0.4143362870015821 1104 56-57 0.14711341607402773 1104 58-59 0.3558836653716355 1104 60-61 0.3241480246605235 1104 62-63 0.6543614308448653 1104 64-65 0.3111667918415719 1104 66-67 0.40559484114556454 1104 68-69 0.3385090207541275 1104 70-71 0.18988563749208964 1104 72-73 0.49577891710392663 1104 74-75 0.45686180057018433 1104 76-77 0.6381140631753652 1104 78-79 0.27009547266828093 1104 80-81 0.6034600399529317 1104 82-83 0.6505409090958132 1104 84-85 0.39750816458443694 1104 86-87 0.5381853173132889 1104 88-89 0.384871590411656 1104 90-91 0.5559943148787987 1104 92-93 0.8283327058716452 1104 94-95 0.9583381232664756 1104 96-97 0.9948754497716159 1104 98-99 0.7705715392917156 1104 100-101 1.2886154586477083 1105 1 0.027249882145213178 1105 2 -0.08325047450308887 1105 3 0.05111475940235266 1105 4 0.03423975831058357 1105 5 0.01657950309201084 1105 6 -0.12586092294319684 1105 7 -0.17914569291011162 1105 8 -0.13863605425017766 1105 9 0.050096534876090004 1105 10-11 -0.09659142257418551 1105 12-13 -0.05919859107599024 1105 14-15 0.0384756239192896 1105 16-17 -0.12684753039654595 1105 18-19 -0.1386946267430389 1105 20-21 -0.32639475338363155 1105 22-23 -0.14866203512838183 1105 24-25 -0.2305043415173671 1105 26-27 -0.11940720702779117 1105 28-29 -0.049134641637991194 1105 30-31 -0.18067141816726462 1105 32-33 -0.23047651713149975 1105 34-35 -0.014050643492382164 1105 36-37 -0.1575680772100725 1105 38-39 -0.0885493732594611 1105 40-41 -0.17066097246625134 1105 42-43 -0.2087058708007845 1105 44-45 0.1124865870886893 1105 46-47 0.1453787902580359 1105 48-49 0.09317172978686727 1105 50-51 0.27049175572613393 1105 52-53 0.31867770931194883 1105 54-55 0.4641473003609775 1105 56-57 0.5013297731936106 1105 58-59 0.577626089614057 1105 60-61 0.3091146261593778 1105 62-63 0.514784206993447 1105 64-65 0.6203937387298097 1105 66-67 0.502257434824287 1105 68-69 0.5076316991510019 1105 70-71 0.5858558232203386 1105 72-73 0.5019820777425679 1105 74-75 0.5991605949657526 1105 76-77 0.6716705962480276 1105 78-79 0.4949667662492523 1105 80-81 0.4272658398877631 1105 82-83 0.32969861518900245 1105 84-85 0.5305954894459539 1105 86-87 0.7021752163031891 1105 88-89 0.6792940407212029 1105 90-91 0.6274744386976288 1105 92-93 0.6167613471228677 1105 94-95 0.6635947215069429 1105 96-97 0.5754587016451858 1105 98-99 0.7485529664666899 1105 100-101 1.3437743051775222 1106 1 -0.16540007079395025 1106 2 -0.05448004582164856 1106 3 0.012435885439828098 1106 4 0.01811928256830697 1106 5 -0.04793662594024539 1106 6 -0.19518581384761546 1106 7 -0.12592587601954364 1106 8 -0.1176743018757449 1106 9 -0.17502539547387386 1106 10-11 -0.2816175199473321 1106 12-13 -0.26076871172286076 1106 14-15 -0.3116920837901418 1106 16-17 -0.03918174790317863 1106 18-19 -0.1033194235257966 1106 20-21 -0.08320402864674037 1106 22-23 -0.1901123645542384 1106 24-25 -0.19022368779955912 1106 26-27 -0.15816308963239578 1106 28-29 -0.16804283022359812 1106 30-31 -0.1134582753590152 1106 32-33 -0.09129024178282208 1106 34-35 -0.14565327889923907 1106 36-37 -0.1673781225596045 1106 38-39 -0.45887708781409486 1106 40-41 -0.1363074172339509 1106 42-43 -0.44621079079736603 1106 44-45 -0.23258485981813237 1106 46-47 -0.33958570020145373 1106 48-49 -0.1934552916899932 1106 50-51 0.048463861484663084 1106 52-53 -0.061958039811010224 1106 54-55 -0.13977243952093943 1106 56-57 0.12656978346142722 1106 58-59 -0.1146020270968151 1106 60-61 -0.017928241984712656 1106 62-63 0.15903850664263075 1106 64-65 0.02459584363268874 1106 66-67 0.05177313603075362 1106 68-69 0.09374875228695956 1106 70-71 -0.1642006497838544 1106 72-73 -0.032526350425481354 1106 74-75 -0.13931548957262052 1106 76-77 -0.03244765480014422 1106 78-79 0.1190157095195552 1106 80-81 -0.11361120298037974 1106 82-83 -0.28406710740875596 1106 84-85 -0.2705818871263048 1106 86-87 -0.14273194550052537 1106 88-89 0.09334726224939516 1106 90-91 -0.18818655214689883 1106 92-93 -0.08536494705014519 1106 94-95 -0.16966103274992506 1106 96-97 -0.24360350422458055 1106 98-99 -0.21171485247743504 1106 100-101 0.35457177244884974 1107 1 -0.02573335047033254 1107 2 0.043950138315018705 1107 3 -0.04260215267527201 1107 4 3.390007993075983E-4 1107 5 0.09982062999642949 1107 6 0.004456550884363253 1107 7 -0.07403376849100596 1107 8 -0.04731253735512553 1107 9 0.023924094668416274 1107 10-11 0.025712603300355852 1107 12-13 0.030936739590451623 1107 14-15 0.11424207212764514 1107 16-17 0.1821234458567531 1107 18-19 0.11246376971623562 1107 20-21 0.20104077304414858 1107 22-23 0.17918629238884876 1107 24-25 0.15059362426695344 1107 26-27 0.09787681714448127 1107 28-29 0.08073939019450194 1107 30-31 0.1372101036643798 1107 32-33 0.04183091328810207 1107 34-35 -0.05340122652072665 1107 36-37 -0.1068233329765178 1107 38-39 -0.1847524543727488 1107 40-41 -0.02869314541714374 1107 42-43 0.08977144641769996 1107 44-45 0.20605199691080145 1107 46-47 0.04632270037804176 1107 48-49 0.19742570884891109 1107 50-51 0.25975211831018186 1107 52-53 0.34236227821142506 1107 54-55 0.21935880772331018 1107 56-57 0.2817247004214636 1107 58-59 0.17936751441617105 1107 60-61 0.30791877970435877 1107 62-63 0.16907818946003772 1107 64-65 0.23043033758928289 1107 66-67 0.5762109627841099 1107 68-69 0.6375065821197659 1107 70-71 0.5853391835063242 1107 72-73 0.532210182262105 1107 74-75 0.6904543220914654 1107 76-77 0.8083154170838434 1107 78-79 0.553143632365515 1107 80-81 0.8638808709542367 1107 82-83 0.6336470363312294 1107 84-85 0.5397052009107632 1107 86-87 0.6320960606303672 1107 88-89 0.6491919043691716 1107 90-91 0.38751699551097474 1107 92-93 0.7387078957653053 1107 94-95 0.8219894785075397 1107 96-97 1.113482363233409 1107 98-99 1.364728811219436 1107 100-101 2.0656553163274083 1108 1 -0.14816897256846318 1108 2 -0.04278702530746159 1108 3 -0.06326716941318722 1108 4 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>>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 4.0 5 21.0 6 35.0 7 89.0 8 215.0 9 414.0 10 854.0 11 1556.0 12 2746.0 13 3898.0 14 5125.0 15 7580.0 16 11162.0 17 16424.0 18 13623.0 19 6372.0 20 4307.0 21 4006.0 22 4040.0 23 4495.0 24 4935.0 25 5755.0 26 6527.0 27 7864.0 28 9520.0 29 11795.0 30 14370.0 31 17758.0 32 22255.0 33 28765.0 34 39275.0 35 58027.0 36 83501.0 37 147114.0 38 158165.0 39 18545.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.02611427667423 23.63690358894369 16.439032919697695 18.897949214684388 2 14.83557398304086 23.842361799638077 40.93739816541749 20.38466605190357 3 17.145372983241927 29.65492376706488 33.445700933931455 19.75400231576174 4 10.746243820590866 20.581575133988313 41.42329212571674 27.248888919704083 5 9.911568974674363 41.249460913264926 35.92695877425197 12.912011337808735 6 24.659257153554417 42.48299579141504 18.902994543399732 13.954752511630808 7 23.419284609891214 38.022727745460344 22.837709475902905 15.720278168745535 8 24.78891207732148 35.42546921908909 21.182563839449763 18.60305486413967 9 26.386787677928847 16.868452253014304 23.758883442303556 32.98587662675329 10-11 22.135554252069284 29.79832016673531 29.079114683218172 18.98701089797724 12-13 24.24517044292348 29.031426009888968 29.333184488005585 17.390219059181966 14-15 21.502194426918305 29.269425704955314 30.0581713802356 19.170208487890786 16-17 17.125890077584952 32.486254498055175 31.057207635080324 19.33064778927955 18-19 16.815758273301487 29.50460725651568 36.8618655055502 16.81776896463263 20-21 17.757606138575554 28.492694598655333 37.21161292917023 16.538086333598883 22-23 17.936637691650077 28.358699473124787 36.71784268902575 16.986820146199378 24-25 17.581374516218048 29.103809102519477 37.04088382161048 16.273932559651996 26-27 17.26475696550959 28.964871713981633 37.57773255632556 16.192638764183222 28-29 16.50683483042063 28.9850892185447 38.00089443887828 16.507181512156397 30-31 17.385537532457178 28.602876685259847 37.50985415503871 16.50173162724427 32-33 17.095244368330917 29.064057852442993 37.199592314999066 16.641105464227028 34-35 17.49481726975851 29.022804013062558 36.986805704816646 16.495573012362286 36-37 17.903346694039822 28.54261524562692 36.47743274752841 17.076605312804855 38-39 16.79469285847507 30.00520702247756 36.45622946398176 16.743870655065614 40-41 17.051159649695727 29.667077818661863 36.811554411078504 16.47020812056391 42-43 17.009155138089827 29.388180489979828 36.196183313559494 17.406481058370854 44-45 17.61171863516478 29.506255356087983 35.34525793810373 17.536768070643507 46-47 18.22920494491399 30.10677464310229 34.26710300979692 17.3969174021868 48-49 17.815973209271366 30.330169383411103 33.94421371568825 17.909643691629284 50-51 17.88655540841301 30.420650493312717 33.294483078992435 18.398311019281838 52-53 18.09414195480798 31.297727918795804 32.77142599615889 17.83670413023733 54-55 18.53760339460164 30.603068730976435 32.43369918671002 18.425628687711903 56-57 18.5279659430489 31.38945704400641 31.829867779711428 18.25270923323326 58-59 18.613662994266065 30.60057270035846 32.33572998495448 18.450034320420997 60-61 18.817713497285567 30.969707895083516 32.461848865346084 17.750729742284836 62-63 18.577678552856916 30.569580320185263 32.76941530482774 18.083325822130085 64-65 18.57067580028982 30.735566356280636 32.431896497930374 18.261861345499174 66-67 18.526232588453084 31.11953906634588 31.53498949587115 18.819238849329885 68-69 18.486018761830145 30.571244340597243 32.21127512497486 18.731461772597743 70-71 18.487544113874463 30.992449507380627 32.63955237850918 17.880454000235737 72-73 18.700122721505384 30.229426814302258 32.73911626649287 18.33133419769949 74-75 18.004770191847687 31.247668638068625 32.19220822442089 18.5553529456628 76-77 17.753503109638142 30.993212183402786 32.96292701190468 18.290357695054393 78-79 17.620728147598612 30.82729548149124 32.403400148375155 19.148576222534995 80-81 17.51859889481311 30.849898425420687 32.38946397742479 19.242038702341414 82-83 17.493593512530094 31.34325460656956 32.12167056787549 19.04148131302486 84-85 17.3166764428137 30.740227666357434 31.92868820747064 20.014407683358222 86-87 16.923642263345098 30.88248549182203 32.22375527806474 19.970116966768124 88-89 17.717934673331992 30.176386163670276 32.287750729742285 19.817928433255446 90-91 17.25213874932568 29.86080968077892 32.83515855659209 20.051893013303303 92-93 17.290435665285035 29.742195202786746 32.83755843284193 20.129810699086292 94-95 17.086092256065008 29.321426342829803 32.910025029640366 20.682456371464824 96-97 17.513329496841827 29.237670648759956 32.42184304127464 20.827156813123572 98-99 16.995195141060396 29.258817574828917 32.307372303766925 21.438614980343758 100-101 16.87277810482873 29.402897417175794 32.693191769965075 21.031132708030405 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1961.0 1 1306.5 2 817.5 3 985.5 4 783.0 5 740.0 6 1394.0 7 2706.0 8 4037.5 9 4401.5 10 3494.5 11 2586.5 12 2407.0 13 2357.5 14 2307.0 15 2248.5 16 2259.0 17 2414.0 18 2579.5 19 2813.5 20 3175.5 21 3545.0 22 3945.0 23 4378.0 24 4958.5 25 6193.0 26 8957.5 27 12320.0 28 13260.5 29 13956.0 30 16033.0 31 17997.0 32 21409.5 33 25728.0 34 30945.5 35 35129.0 36 39916.0 37 44161.5 38 41548.0 39 36856.0 40 32987.5 41 28941.5 42 25798.5 43 24503.0 44 23640.0 45 22535.5 46 20327.5 47 17765.0 48 18521.0 49 16686.0 50 11946.0 51 8799.0 52 6433.0 53 5171.0 54 4340.5 55 3708.5 56 3385.0 57 3030.5 58 2750.5 59 2632.0 60 2715.0 61 2627.0 62 1822.5 63 868.5 64 324.0 65 183.0 66 128.5 67 103.0 68 87.5 69 64.5 70 39.5 71 27.0 72 25.0 73 26.0 74 33.0 75 36.5 76 27.0 77 19.0 78 15.0 79 10.0 80 9.0 81 5.5 82 5.5 83 7.0 84 3.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07529692364226334 2 0.0 3 0.0 4 0.0 5 0.0024960306179755806 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.7733673533062006E-4 12-13 0.005338732155114437 14-15 0.0 16-17 0.0 18-19 0.0 20-21 6.933418383265501E-5 22-23 9.013443898245152E-4 24-25 5.546734706612401E-4 26-27 3.466709191632751E-4 28-29 0.0031200382724694757 30-31 3.466709191632751E-4 32-33 0.0 34-35 0.0 36-37 6.933418383265501E-5 38-39 4.853392868285851E-4 40-41 6.933418383265501E-5 42-43 0.010330793391065596 44-45 4.160051029959301E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.3866836766531003E-4 84-85 2.7733673533062006E-4 86-87 0.0 88-89 0.0 90-91 0.006378744912604261 92-93 0.0024960306179755806 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.007557426037759396 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 721145.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.86162558694993 #Duplication Level Percentage of deduplicated Percentage of total 1 75.46325984324753 24.043821306962666 2 12.161104494050326 7.749451162264114 3 4.189423872780475 4.004455645786837 4 1.9630150250187266 2.501793989948153 5 1.1102124907381221 1.7686587350926584 6 0.7264894586914404 1.3888281075415563 7 0.48913365650680996 1.090921539791703 8 0.375883571314772 0.9581009290813488 9 0.2862431460469079 0.8208154751559487 >10 2.4386510279568276 16.334995379067855 >50 0.4530104908564943 10.152871583236923 >100 0.32046459169008656 19.700196648374565 >500 0.017004243640698472 3.5649311152913956 >1k 0.004796068719171364 2.256456087042247 >5k 8.720124943947934E-4 2.1135087673619863 >10k+ 4.360062471973967E-4 1.5501935280000267 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11159 1.5474003147771946 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8949 1.2409432222368593 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6265 0.8687573234231672 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2822 0.39132213355150486 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2105 0.2918969139354776 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1888 0.2618058781521054 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1560 0.21632265355788363 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1243 0.17236478100798036 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1233 0.17097809733132727 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1161 0.16099397485942493 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1112 0.15419922484382476 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1086 0.15059384728452668 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1021 0.14158040338628156 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1012 0.14033238807729373 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 971 0.13464698500301603 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 961 0.13326030132636293 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 924 0.12812957172274647 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 881 0.12216683191313815 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 866 0.1200868063981585 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 863 0.11967080129516255 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 846 0.11731343904485228 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 835 0.11578808700053386 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 791 0.10968667882326023 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 790 0.10954801045559492 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.3866836766531003E-4 0.0 14-15 0.0 0.0 0.0 1.3866836766531003E-4 0.0 16-17 0.0 0.0 0.0 1.3866836766531003E-4 0.0 18-19 0.0 0.0 0.0 2.7733673533062006E-4 0.0 20-21 0.0 0.0 0.0 4.160051029959301E-4 0.0 22-23 0.0 0.0 0.0 9.706785736571702E-4 0.0 24-25 0.0 0.0 0.0 0.0012480153089877903 0.0 26-27 0.0 0.0 0.0 0.0024266964341429255 0.0 28-29 0.0 0.0 0.0 0.0054080663389470904 0.0 30-31 0.0 0.0 0.0 0.013728168398865693 0.0 32-33 0.0 0.0 0.0 0.019829576576139332 0.0 34-35 0.0 0.0 0.0 0.025306977098919083 0.0 36-37 0.0 0.0 0.0 0.033211074055841755 0.0 38-39 0.0 0.0 0.0 0.04534455622655638 0.0 40-41 0.0 0.0 0.0 0.06108341595656906 0.0 42-43 0.0 0.0 0.0 0.07904096956922671 0.0 44-45 0.0 0.0 0.0 0.08722240326148001 0.0 46-47 0.0 0.0 0.0 0.09873187777770073 0.0 48-49 0.0 0.0 0.0 0.12001747221432582 0.0 50-51 0.0 0.0 0.0 0.14899916105637562 0.0 52-53 0.0 0.0 0.0 0.20807188568179769 0.0 54-55 0.0 0.0 0.0 0.25265376588619487 0.0 56-57 0.0 0.0 0.0 0.3564470390836794 0.0 58-59 0.0 0.0 0.0 0.5125182868909859 0.0 60-61 0.0 0.0 0.0 0.6527813407844469 0.0 62-63 0.0 6.933418383265501E-5 0.0 0.7248888919704082 0.0 64-65 0.0 1.3866836766531003E-4 0.0 0.8501757621560158 0.0 66-67 0.0 1.3866836766531003E-4 0.0 1.0866746632092021 0.0 68-69 0.0 1.3866836766531003E-4 0.0 1.4313348910413302 0.0 70-71 0.0 1.3866836766531003E-4 0.0 1.7660803305853885 0.0 72-73 0.0 1.3866836766531003E-4 0.0 2.120378009970256 0.0 74-75 0.0 1.3866836766531003E-4 0.0 2.557876709954309 0.0 76-77 0.0 1.3866836766531003E-4 0.0 2.880558001511485 0.0 78-79 0.0 1.3866836766531003E-4 0.0 3.283597612130709 0.0 80-81 0.0 1.3866836766531003E-4 0.0 3.8137267817151894 0.0 82-83 0.0 1.3866836766531003E-4 0.0 4.255038861810038 0.0 84-85 0.0 1.3866836766531003E-4 0.0 4.770399850238163 0.0 86-87 0.0 1.3866836766531003E-4 0.0 5.461245657946737 0.0 88-89 0.0 1.3866836766531003E-4 0.0 6.36591808859522 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGCG 85 3.6389793E-7 39.11656 5 CATTCCG 245 0.0 36.835716 9 GTACAGT 55 0.002579341 34.5445 6 GGTATCA 6020 0.0 31.341267 1 GGCATTC 320 0.0 31.171011 7 GTATCAA 8960 0.0 29.915318 1 ATAAGGT 80 4.4927277E-4 29.686676 3 TTAGGCA 345 0.0 27.535467 4 GTCGGGA 190 3.947207E-10 27.499237 2 TAGGCAT 335 0.0 26.93955 5 AGGGGTC 145 7.882627E-7 26.20617 5 GTACATG 5095 0.0 25.464844 1 GGGGTCC 150 1.0594504E-6 25.332628 6 TAGGGCG 75 1.0606818E-6 25.332628 30-31 TAAGGCC 95 0.0012263099 24.999308 3 CATGGGT 605 0.0 24.338167 4 ACATGGG 5095 0.0 24.052326 3 TACATGG 5355 0.0 23.948917 2 TATCAAC 11175 0.0 23.929983 2 CATGGGG 1810 0.0 23.880552 4 >>END_MODULE