FastQCFastQC Report
Thu 26 May 2016
SRR937195_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937195_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847699
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51720.6101222249878789No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37410.44131230542916766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24970.2945621028218743No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12950.1527664890485892No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9370.11053451755870894No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30150.045.414831
GTATCAA51000.037.3823621
TATCAAC65300.029.249792
ATCAACG65900.028.911383
TCAACGC67050.028.4863724
CAACGCA67250.028.3343565
AACGCAG68900.027.6509026
GTACATG56750.026.64121
TACCTGG6150.026.2671932
TACATGG57100.026.2110442
ATGGGAG15650.025.505055
ACATGGG56750.025.3680233
CCTATAC2251.0186341E-1025.3401153
ACGCAGA77700.024.398427
CGCAGAG78050.024.2281388
ATAACGC1000.00165071123.756363
TATAACG1000.00165071123.756362
GAGTACT40000.022.9788412-13
CATGGGA39350.022.6998484
GCAGAGT81350.022.6028569
Produced by FastQC (version 0.11.5)