##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937195_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 847699 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.861076868086432 33.0 31.0 34.0 30.0 34.0 2 31.96366280955858 33.0 31.0 34.0 30.0 34.0 3 31.99580157579518 34.0 31.0 34.0 30.0 34.0 4 35.61959256764489 37.0 35.0 37.0 33.0 37.0 5 35.636081911150065 37.0 35.0 37.0 33.0 37.0 6 35.68688650098679 37.0 35.0 37.0 35.0 37.0 7 35.63299355077687 37.0 35.0 37.0 33.0 37.0 8 35.61870428064679 37.0 35.0 37.0 33.0 37.0 9 37.232800793677946 39.0 37.0 39.0 34.0 39.0 10-11 37.12263079229774 39.0 37.0 39.0 34.0 39.0 12-13 36.9839306168817 39.0 37.0 39.0 33.0 39.0 14-15 38.10292627453849 40.0 38.0 41.0 33.0 41.0 16-17 38.0369895446379 40.0 38.0 41.0 33.0 41.0 18-19 37.98999467971532 40.0 38.0 41.0 33.0 41.0 20-21 37.88385971907481 40.0 38.0 41.0 33.0 41.0 22-23 37.94953456356561 40.0 38.0 41.0 33.0 41.0 24-25 37.92541456342404 40.0 38.0 41.0 33.0 41.0 26-27 37.83687842028833 40.0 38.0 41.0 33.0 41.0 28-29 37.6929328688603 40.0 37.0 41.0 33.0 41.0 30-31 37.42581505935479 39.5 37.0 41.0 32.0 41.0 32-33 37.170286858896844 39.0 36.0 41.0 31.5 41.0 34-35 37.17741202950576 39.5 36.0 41.0 32.0 41.0 36-37 37.04605644220413 39.5 36.0 41.0 31.0 41.0 38-39 36.89609342467078 39.0 36.0 41.0 31.0 41.0 40-41 36.65116273582958 39.0 35.5 41.0 30.5 41.0 42-43 36.355620922048985 39.0 35.0 41.0 30.0 41.0 44-45 36.07005906577689 39.0 35.0 41.0 29.5 41.0 46-47 35.602578863488105 39.0 35.0 40.0 27.5 41.0 48-49 35.208581701759705 39.0 35.0 40.0 26.0 41.0 50-51 34.22847909458428 37.5 34.0 39.5 24.5 40.5 52-53 34.312522487345156 38.0 34.0 39.5 24.0 40.5 54-55 34.601302467031346 38.0 34.0 40.0 24.0 41.0 56-57 34.3462850610889 38.0 34.0 40.0 23.0 41.0 58-59 34.066739491258105 38.0 34.0 40.0 22.5 41.0 60-61 33.73057299819865 37.5 33.0 40.0 22.0 41.0 62-63 33.34583148027778 37.0 33.0 40.0 20.5 41.0 64-65 32.910551976586035 36.5 32.0 39.0 18.5 41.0 66-67 32.470964929768705 36.0 32.0 39.0 16.5 40.5 68-69 31.97789014732824 35.0 31.0 39.0 12.0 40.0 70-71 31.515086722999555 35.0 31.0 38.0 8.0 40.0 72-73 31.020174024034475 35.0 30.5 37.5 7.0 39.5 74-75 30.468408008031155 34.5 30.0 37.0 2.0 39.0 76-77 29.95316733887854 34.0 29.0 36.0 2.0 39.0 78-79 29.410436369513235 34.0 29.0 36.0 2.0 38.5 80-81 28.841359963855098 34.0 28.5 35.0 2.0 37.0 82-83 28.295550661260663 34.0 27.5 35.0 2.0 37.0 84-85 27.72004390709438 33.0 26.0 35.0 2.0 36.0 86-87 27.12255529380122 33.0 25.5 35.0 2.0 36.0 88-89 26.576502980421118 32.5 25.0 35.0 2.0 35.0 90-91 25.997998700010264 32.0 23.5 35.0 2.0 35.0 92-93 25.32147200834258 32.0 20.0 34.0 2.0 35.0 94-95 24.5211100874249 31.0 15.5 34.0 2.0 35.0 96-97 23.634082380656345 31.0 2.0 34.0 2.0 35.0 98-99 22.43172163704334 30.5 2.0 34.0 2.0 35.0 100-101 19.32139709967807 26.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.016211461855064613 1101 2 0.13249659197353836 1101 3 0.16544004389261602 1101 4 0.04529900738052106 1101 5 0.08026945091843629 1101 6 0.11661377864520972 1101 7 0.049695793494244356 1101 8 0.14198384510704898 1101 9 0.10994044552810323 1101 10-11 0.17526237512007015 1101 12-13 0.2643301136397511 1101 14-15 0.3272377659292047 1101 16-17 0.3430696613039004 1101 18-19 0.30675213194281525 1101 20-21 0.26688853095842546 1101 22-23 0.2853498613701362 1101 24-25 0.20110082519975236 1101 26-27 0.16389604830622062 1101 28-29 0.18897375293096985 1101 30-31 0.2764265334751599 1101 32-33 0.11802973282082974 1101 34-35 0.03345025992489781 1101 36-37 0.02112263339175513 1101 38-39 0.09288973535369394 1101 40-41 0.0773196005079484 1101 42-43 0.2589706123963822 1101 44-45 0.15484370453727792 1101 46-47 0.20802121500223336 1101 48-49 0.1667653613679434 1101 50-51 0.3028018988021799 1101 52-53 -0.05932834569842527 1101 54-55 0.10373039175829746 1101 56-57 0.1730876611653187 1101 58-59 0.03201753332957935 1101 60-61 0.13085922326843757 1101 62-63 0.3039546375591087 1101 64-65 0.1464759116069061 1101 66-67 0.08762288849599287 1101 68-69 0.1148676240203379 1101 70-71 0.09373499181918632 1101 72-73 0.036667578568973624 1101 74-75 0.1834453558080611 1101 76-77 0.30355742650484174 1101 78-79 0.2301752120728615 1101 80-81 0.32776517145560646 1101 82-83 0.21448329172249103 1101 84-85 0.4334975154925722 1101 86-87 0.6326306764510008 1101 88-89 0.7798007795562789 1101 90-91 0.6184999650995664 1101 92-93 1.0210425440261446 1101 94-95 1.152097579822879 1101 96-97 1.0104616712543226 1101 98-99 1.3677045310251401 1101 100-101 1.6318513402970076 1102 1 0.16741711490822553 1102 2 0.19978700414737105 1102 3 0.17257460721241102 1102 4 0.14140774565247227 1102 5 0.10764365154393118 1102 6 -0.0018599411886128792 1102 7 -0.02575903309399763 1102 8 -0.006238859462804669 1102 9 0.21134139310780142 1102 10-11 0.17165643757630988 1102 12-13 0.21236128924540054 1102 14-15 0.39477834731369654 1102 16-17 0.43393651585274995 1102 18-19 0.4699740488078632 1102 20-21 0.3415753453635091 1102 22-23 0.309692127859428 1102 24-25 0.1373857176051203 1102 26-27 0.2975396498092806 1102 28-29 0.2981598496494442 1102 30-31 0.49535960190628714 1102 32-33 0.5178008608141056 1102 34-35 0.4670394514480236 1102 36-37 0.4936368412524672 1102 38-39 0.31964733816375457 1102 40-41 0.5500066658116012 1102 42-43 0.4553643831100942 1102 44-45 0.640441835051206 1102 46-47 0.6189201320539937 1102 48-49 0.7897946406472229 1102 50-51 0.6303492486131361 1102 52-53 0.6822767632965778 1102 54-55 0.7471612254417224 1102 56-57 0.7602435508633079 1102 58-59 0.8641000206096052 1102 60-61 0.8262851381729774 1102 62-63 0.8368223498309604 1102 64-65 0.5119621880387797 1102 66-67 0.7451106061359098 1102 68-69 0.9665603141197714 1102 70-71 1.142294804870577 1102 72-73 1.0729297150577963 1102 74-75 1.0630745802397463 1102 76-77 1.3204288964203563 1102 78-79 1.0560041984426505 1102 80-81 1.4596816572419122 1102 82-83 1.3847836114722938 1102 84-85 1.3676169490452956 1102 86-87 1.4665759397194549 1102 88-89 1.4568089891907867 1102 90-91 1.22727251679634 1102 92-93 1.2801697087556434 1102 94-95 1.5544506291156956 1102 96-97 1.4065947700266719 1102 98-99 1.8418824006045647 1102 100-101 1.8945735022257715 1103 1 0.29231322824857386 1103 2 0.1618732430607146 1103 3 0.11040168786741589 1103 4 0.15169252495824992 1103 5 0.22189462872782428 1103 6 0.14426313853141437 1103 7 0.1857136252357634 1103 8 0.15206817755980495 1103 9 0.1496554424799399 1103 10-11 0.13079732736554917 1103 12-13 0.17809573546458068 1103 14-15 0.2708626643238361 1103 16-17 0.10524642923724059 1103 18-19 0.14326592487108059 1103 20-21 0.2708098630047573 1103 22-23 0.13698380264224141 1103 24-25 0.237507297461498 1103 26-27 0.16415323688578098 1103 28-29 -0.040584517745720916 1103 30-31 0.15308714615550656 1103 32-33 0.046210615771450136 1103 34-35 0.34105415141036843 1103 36-37 0.1323709823045789 1103 38-39 0.16924299180563906 1103 40-41 0.530746241434592 1103 42-43 0.1246038678322634 1103 44-45 0.15993032311073563 1103 46-47 0.00373092847510037 1103 48-49 0.2816492772387065 1103 50-51 0.28346195265302754 1103 52-53 0.19979708612437008 1103 54-55 0.42303160086213865 1103 56-57 0.41528627425209663 1103 58-59 0.3259078757396594 1103 60-61 0.1773595534858714 1103 62-63 0.348102168548742 1103 64-65 0.2651128756386072 1103 66-67 0.5670001746166946 1103 68-69 0.7085350696614761 1103 70-71 0.5818376386389339 1103 72-73 0.6551965869006153 1103 74-75 0.8064672263628268 1103 76-77 0.7575936996200632 1103 78-79 0.8016831626926582 1103 80-81 0.742965969057721 1103 82-83 0.8813764536814439 1103 84-85 0.9849447569069198 1103 86-87 1.0663363511106283 1103 88-89 0.9045499413120233 1103 90-91 0.5968484969020444 1103 92-93 1.1346309914961736 1103 94-95 0.9259907163804897 1103 96-97 1.0491637897261796 1103 98-99 1.1351552524232282 1103 100-101 1.1415705284701438 1104 1 0.2588677288668464 1104 2 0.25392342996464734 1104 3 0.14744491029020423 1104 4 0.17758902424048273 1104 5 0.16795720956555016 1104 6 0.2848461650959351 1104 7 0.35835280825948246 1104 8 0.20811448425281043 1104 9 0.34431624382166603 1104 10-11 0.3266316082473395 1104 12-13 0.3823442466290672 1104 14-15 0.49136133538713267 1104 16-17 0.33584270963578433 1104 18-19 0.3294867344564807 1104 20-21 0.40737143470337855 1104 22-23 0.4320610515300487 1104 24-25 0.47307465394343495 1104 26-27 0.5046213139783262 1104 28-29 0.4803844334602658 1104 30-31 0.38567061333006336 1104 32-33 0.5795974855925081 1104 34-35 0.6223019305936717 1104 36-37 0.7078538766860945 1104 38-39 0.7568328904653256 1104 40-41 0.7138916640648887 1104 42-43 0.4983708704048553 1104 44-45 0.5634758125431958 1104 46-47 0.5539729086618692 1104 48-49 0.6629830464443316 1104 50-51 0.6818835738136784 1104 52-53 0.5329400214399769 1104 54-55 0.6626843717307125 1104 56-57 0.8108563667802002 1104 58-59 0.8369648778858547 1104 60-61 0.8380427477664725 1104 62-63 0.6166877392808701 1104 64-65 0.6172615602044615 1104 66-67 0.6852933082068162 1104 68-69 0.5942109796633197 1104 70-71 0.5804394124496461 1104 72-73 0.792263616478337 1104 74-75 0.7989368811235451 1104 76-77 0.8980274873028797 1104 78-79 0.7972486359220419 1104 80-81 0.8031785278649473 1104 82-83 0.8268207591521168 1104 84-85 0.9971334872393669 1104 86-87 1.0003159124904428 1104 88-89 1.041539686468866 1104 90-91 1.2047579799669883 1104 92-93 1.3584253545304108 1104 94-95 1.425673583756872 1104 96-97 1.4750533600603326 1104 98-99 1.5901250848492872 1104 100-101 1.2707296739709442 1105 1 0.1139455433019414 1105 2 -0.1587206367436913 1105 3 0.0364887586913305 1105 4 0.010018409599929612 1105 5 0.11081491896390361 1105 6 0.06108316061459362 1105 7 0.11363438868283993 1105 8 0.12321545133865897 1105 9 0.050241698329351436 1105 10-11 0.21423691409461298 1105 12-13 0.39062991181454976 1105 14-15 0.3715317320981697 1105 16-17 0.43036538922462597 1105 18-19 0.40398218229786664 1105 20-21 0.42521795491285275 1105 22-23 0.5400938504891286 1105 24-25 0.34806450341343265 1105 26-27 0.4483788796640553 1105 28-29 0.35371526142247944 1105 30-31 0.28122874140236576 1105 32-33 0.44195960362570474 1105 34-35 0.4972795050041441 1105 36-37 0.5722206063647306 1105 38-39 0.6263220969110606 1105 40-41 0.5425469407352921 1105 42-43 0.44571039288615566 1105 44-45 0.38244465088822466 1105 46-47 0.13648633096735097 1105 48-49 0.23168608253866552 1105 50-51 0.10695424982953483 1105 52-53 0.22690484678476963 1105 54-55 0.4845227413006512 1105 56-57 0.6494402781429329 1105 58-59 0.3904255948626414 1105 60-61 0.38643008821430413 1105 62-63 0.4242431328476002 1105 64-65 0.499648077279069 1105 66-67 0.6984871087352076 1105 68-69 0.6001171717698917 1105 70-71 0.7846019795611774 1105 72-73 0.9350943207457192 1105 74-75 0.8295395487772552 1105 76-77 0.6660142264616447 1105 78-79 0.7382334545061049 1105 80-81 0.5927674833329171 1105 82-83 0.7661824371716399 1105 84-85 0.821013293633353 1105 86-87 1.1955360987314236 1105 88-89 1.0779456245761274 1105 90-91 0.9422695232441249 1105 92-93 1.1884587426923474 1105 94-95 1.4686750057753066 1105 96-97 1.6702499404175946 1105 98-99 1.532268008088618 1105 100-101 1.7560350113556744 1106 1 0.047012476368873735 1106 2 -0.5615129663652922 1106 3 -0.07718618425190371 1106 4 0.10153664745962487 1106 5 -0.08131002925860287 1106 6 -0.05287605090559566 1106 7 -0.04843118801687751 1106 8 0.11302239236511014 1106 9 0.09624447603945185 1106 10-11 -0.18122128624163736 1106 12-13 -0.2584567738575103 1106 14-15 -0.23612889995271047 1106 16-17 -0.10911951611393533 1106 18-19 -0.04413474142881313 1106 20-21 0.0685015006354206 1106 22-23 -0.027955985853388654 1106 24-25 -0.024737491339777762 1106 26-27 0.04378753604832042 1106 28-29 0.058348027030852734 1106 30-31 0.2175241533075365 1106 32-33 0.43802857530442907 1106 34-35 0.31854360675604454 1106 36-37 0.32889888743325457 1106 38-39 0.4147003852893434 1106 40-41 0.35544136338581467 1106 42-43 0.2016333317359269 1106 44-45 0.14862156340659993 1106 46-47 0.2881233118726172 1106 48-49 0.4483312260130816 1106 50-51 0.20121364896697713 1106 52-53 0.17423394045532348 1106 54-55 0.25688452585755783 1106 56-57 0.3169841164184817 1106 58-59 0.11109110821107748 1106 60-61 0.2079581536448032 1106 62-63 0.30400564675645825 1106 64-65 0.44279030090910254 1106 66-67 0.5151785473778645 1106 68-69 0.5111208022857134 1106 70-71 0.5984263665562928 1106 72-73 0.4560936466230885 1106 74-75 0.43542147173234724 1106 76-77 0.8501268617937896 1106 78-79 0.9172617432904921 1106 80-81 0.6426160087912045 1106 82-83 0.7792384868130533 1106 84-85 0.7590102560205558 1106 86-87 0.8226325794864451 1106 88-89 0.8732560052523617 1106 90-91 0.8609118565206053 1106 92-93 0.9849427464934237 1106 94-95 1.066406542531233 1106 96-97 1.295583955995511 1106 98-99 1.0194353123290902 1106 100-101 0.9237088009806875 1107 1 -0.14234888690553404 1107 2 -0.007770137967106905 1107 3 -0.13894450369845046 1107 4 -0.15552619072727936 1107 5 -0.2314659885343957 1107 6 -0.2416430986334035 1107 7 -0.30538137815901223 1107 8 -0.32998352359945216 1107 9 -0.21120699790195374 1107 10-11 -0.20621944114435564 1107 12-13 -0.2086838179339665 1107 14-15 -0.23888683484214113 1107 16-17 -0.12651975461703557 1107 18-19 -0.0947292511961777 1107 20-21 -0.23206678932841385 1107 22-23 -0.13744600096377013 1107 24-25 -0.24094793342508325 1107 26-27 -0.1852628409341932 1107 28-29 -0.17428572310024748 1107 30-31 -0.23649990676106825 1107 32-33 -0.31341545609718935 1107 34-35 -0.3024714686598742 1107 36-37 -0.21651963889725323 1107 38-39 -0.17878583863166142 1107 40-41 -0.19449862239722648 1107 42-43 -0.24813923574608054 1107 44-45 -0.20121082407160173 1107 46-47 -0.2237765945129695 1107 48-49 -0.369068235606953 1107 50-51 -0.4634680814623593 1107 52-53 -0.1243960262552335 1107 54-55 -0.0829532609579573 1107 56-57 -0.16020659672133064 1107 58-59 -0.036574940833546066 1107 60-61 -0.017369011236972653 1107 62-63 -0.07714678158579602 1107 64-65 -0.12280193647529103 1107 66-67 0.04075353795816028 1107 68-69 0.14416008537802227 1107 70-71 0.21969587117680334 1107 72-73 0.38927781840747144 1107 74-75 0.2873281370006211 1107 76-77 0.13574015270930673 1107 78-79 0.25615755938673246 1107 80-81 0.4446040525607948 1107 82-83 0.5785657060766454 1107 84-85 0.30890945544255644 1107 86-87 0.23107302557269804 1107 88-89 0.17093234799763124 1107 90-91 0.029790697721818304 1107 92-93 0.23493400655891605 1107 94-95 0.34450931639222304 1107 96-97 0.5571022185742187 1107 98-99 1.0021749127781305 1107 100-101 0.8049136689299878 1108 1 0.11148782403006052 1108 2 0.1716127466870354 1108 3 -0.06820300971725146 1108 4 0.009001727167316176 1108 5 0.008881454198579775 1108 6 0.16389185634364623 1108 7 0.15496473974770453 1108 8 0.23489138602344184 1108 9 0.12788087331366427 1108 10-11 0.2531316476796093 1108 12-13 0.1503701715548047 1108 14-15 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1.7643762152620361 2316 90-91 1.580811831999199 2316 92-93 1.9926014085371406 2316 94-95 2.0502423745767153 2316 96-97 2.4650933948355167 2316 98-99 2.503052542702939 2316 100-101 3.385193087322264 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3217.0 3 537.0 4 544.0 5 570.0 6 651.0 7 784.0 8 984.0 9 1285.0 10 1581.0 11 2009.0 12 3021.0 13 4079.0 14 4524.0 15 5934.0 16 8375.0 17 11135.0 18 9264.0 19 6126.0 20 6019.0 21 6412.0 22 6895.0 23 7882.0 24 8911.0 25 10151.0 26 11802.0 27 13874.0 28 16234.0 29 19541.0 30 24044.0 31 29722.0 32 37722.0 33 48481.0 34 65350.0 35 89804.0 36 122526.0 37 150574.0 38 98338.0 39 8797.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.408401800554834 19.336208069372233 14.681974931828535 21.5734151982444 2 14.982095892225313 20.57009328111105 40.9516849255454 23.49612590111824 3 19.092421710640682 26.17686814180955 29.622534185259685 25.10817596229008 4 12.048863844657438 16.720256854478695 37.7021457755598 33.52873352530407 5 13.073937507538972 38.74948853248439 33.3307710683226 14.845802891654033 6 30.791611004054996 36.702448366770305 17.84352205421637 14.662418574958327 7 28.43131807396706 33.9313813229802 20.12666353771237 17.51063706534037 8 27.22041933405449 34.59429105117247 19.37637802722657 18.808911587546476 9 27.31894647730121 16.802873587680843 20.09950672285202 35.77867321216593 10-11 24.843311850359786 27.809523021230127 26.48643119940263 20.860733929007452 12-13 26.090845405558788 25.057890520852116 27.67749201178797 21.173772061801124 14-15 22.814835832276714 25.9712776679287 26.637347307318315 24.576539192476275 16-17 21.431260879716515 29.847942569874007 26.978918840639675 21.7418777097698 18-19 21.454508703931573 27.535854146054888 30.334977547201703 20.67465960281183 20-21 22.500927968375784 26.4248747538224 30.378197353457836 20.69599992434398 22-23 21.928540863344388 26.817980864479185 30.272256804646997 20.98122146752943 24-25 21.58907008221672 26.856141429306035 30.48650310011999 21.06828538835726 26-27 21.32740721477353 27.245330078696412 30.231479155646312 21.195783550883746 28-29 21.151626100120172 27.405575419965277 30.377769252731053 21.065029227183494 30-31 21.245465638168568 27.053054989335084 30.45500872583841 21.246470646657933 32-33 20.921685846041257 27.329943765328867 30.21598298949967 21.532387399130204 34-35 21.538546114886433 27.204484514875084 30.13764949565168 21.119319874586804 36-37 21.569257921916567 27.06688567650812 29.966539725284107 21.3973166762912 38-39 21.334640622534426 27.324353698558728 30.093537369736488 21.24746830917035 40-41 21.4060715948929 27.216803500551983 30.085350776206273 21.291774128348845 42-43 21.36064642615021 27.246323188250756 29.808728360700208 21.584302024898825 44-45 21.357105874731964 27.633628933687326 29.49387797560647 21.51538721597424 46-47 21.49154021118735 27.804344330901166 28.973653597892856 21.730461860018632 48-49 21.673389718695837 27.96643833511065 28.589069405069008 21.771102541124506 50-51 21.76218396879504 28.1869152469427 28.3928239944287 21.658076789833558 52-53 21.929853087515426 28.219294391995643 28.15277668557346 21.69807583491547 54-55 21.47475135590465 28.193713331461424 28.42874665642041 21.902788656213517 56-57 21.614925773601655 28.581492423700677 28.111471685579588 21.69211011711808 58-59 21.796122615045416 27.93940278556668 28.361628461497094 21.902846137890812 60-61 21.594258739655306 27.976352285701456 28.72639424361117 21.702994731032067 62-63 21.75389223255528 28.168274936055337 28.678118395733556 21.399714435655827 64-65 21.43424025226139 28.06975244842109 28.71967063247948 21.776336666838038 66-67 21.332156138991667 28.361814699446093 28.326297942451035 21.979731219111205 68-69 21.347802653693684 28.190176652885235 28.64599215664026 21.81602853678082 70-71 21.329937995087832 28.337698655654076 28.44194633490217 21.890417014355922 72-73 21.40309113603268 28.27168475896893 28.263706337348943 22.06151776764945 74-75 21.257779117391536 28.534482249132697 28.21480706606857 21.992931567407197 76-77 21.4124735575687 28.38269549371913 28.239186752275426 21.965644196436752 78-79 21.40233201859042 28.314810097178277 28.063249517241296 22.219608366990006 80-81 21.28337245946131 28.59948556825086 28.022936658226772 22.094205314061057 82-83 21.336226001628752 28.592146771987842 27.9592623104863 22.112364915897107 84-85 21.310209281766912 28.19885077032898 28.048283246585637 22.44265670131847 86-87 21.507187283014968 28.111923650814756 28.038292780215397 22.342596285954883 88-89 21.42664647832886 27.88910125255161 28.088443358592396 22.595808910527133 90-91 21.17054051450499 27.97444318300688 28.425842147472363 22.429174155015772 92-93 21.320624361345196 27.89177279639345 28.380238446886086 22.407364395375268 94-95 21.06608104466392 27.912350541101492 28.233725586606973 22.787842827627617 96-97 21.11528377672338 28.002723462201324 28.11720580343317 22.764786957642123 98-99 21.12546774308899 28.124458818807955 27.742403367572926 23.007670070530132 100-101 21.416494746664476 27.426494965319538 28.36251590420072 22.79449438381527 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1441.0 1 821.5 2 240.0 3 286.0 4 276.5 5 291.0 6 466.0 7 853.0 8 1374.5 9 1678.5 10 1572.0 11 1462.5 12 1514.0 13 1606.5 14 1700.5 15 1757.5 16 1820.0 17 1885.5 18 1979.5 19 2067.0 20 2222.5 21 2482.5 22 2839.0 23 3329.0 24 3821.0 25 4504.0 26 5522.5 27 6776.5 28 8066.5 29 9816.5 30 11890.0 31 13321.0 32 14999.5 33 16756.5 34 18393.5 35 19775.5 36 21387.0 37 23405.5 38 24515.0 39 24786.5 40 25460.5 41 27190.5 42 28315.5 43 29419.5 44 30921.0 45 31906.5 46 31341.0 47 30850.0 48 32447.5 49 32272.5 50 30695.0 51 29156.0 52 26817.0 53 25705.0 54 25123.5 55 23331.0 56 20901.5 57 18486.5 58 16681.5 59 14744.0 60 12276.0 61 10048.0 62 8030.0 63 6018.5 64 4236.0 65 3204.0 66 2463.0 67 1809.0 68 1322.5 69 916.0 70 647.5 71 482.0 72 371.5 73 304.0 74 225.0 75 172.0 76 144.5 77 99.5 78 59.0 79 37.0 80 22.0 81 11.0 82 11.0 83 9.5 84 5.0 85 5.0 86 4.5 87 2.5 88 1.5 89 1.5 90 1.5 91 1.0 92 0.0 93 1.0 94 3.0 95 3.5 96 1.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2835912275465702 2 0.24572401288664963 3 0.2460779120890788 4 0.24584197928745935 5 0.24690367689474682 6 0.21576054708098039 7 0.21658631188664845 8 0.216350379085029 9 0.2273212543603331 10-11 0.2348121208117504 12-13 0.22667243915587962 14-15 0.21723512709110193 16-17 0.21251647105871305 18-19 0.20077881417814575 20-21 0.2082106974291582 22-23 0.20443577260324713 24-25 0.2080927310283485 26-27 0.20366899099798397 28-29 0.21534766467814637 30-31 0.22826498556681088 32-33 0.2204202199129644 34-35 0.21882767350203314 36-37 0.2088595126336117 38-39 0.1974167717550687 40-41 0.1959421917449472 42-43 0.2070900166214659 44-45 0.20490763820648603 46-47 0.2141090174696443 48-49 0.21859174070041368 50-51 0.2283829519676206 52-53 0.2429518024676212 54-55 0.21959445510729633 56-57 0.19729880535425898 58-59 0.18727166128543268 60-61 0.1902798045060806 62-63 0.19523439334008888 64-65 0.1878614932894813 66-67 0.19104658611134376 68-69 0.19263913252227502 70-71 0.19311099812551388 72-73 0.19664999014980555 74-75 0.1986554189635708 76-77 0.20732594942308533 78-79 0.2099212102408992 80-81 0.2025483101902916 82-83 0.1948804941376597 84-85 0.1848533500688334 86-87 0.18715369488462297 88-89 0.19653202374899584 90-91 0.1961191413461618 92-93 0.19847846936235622 94-95 0.19429066213361113 96-97 0.2025483101902916 98-99 0.20626425181579783 100-101 0.19081065330972433 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 847699.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.156760324692804 #Duplication Level Percentage of deduplicated Percentage of total 1 81.00506874514042 49.54007574331817 2 11.045029931138 13.5095649655533 3 3.329482306909156 6.108610543468457 4 1.457775004773359 3.5661118629700894 5 0.7833184228366732 2.3952608521669396 6 0.5063751018801218 1.8580956444044703 7 0.3305417184995104 1.4150402458910645 8 0.22810548899633828 1.116015417543673 9 0.1938590368459403 1.0670211587846665 >10 1.016528508885803 11.418703711441337 >50 0.07320606787326364 3.0451799607669954 >100 0.029357668347013942 3.1900595223670543 >500 5.794276604541318E-4 0.26954922048180957 >1k 5.794276604541318E-4 0.8897959149384186 >5k 1.931425534847106E-4 0.6109152359035578 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5172 0.6101222249878789 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3741 0.44131230542916766 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2497 0.2945621028218743 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1295 0.1527664890485892 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 937 0.11053451755870894 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1796640080972138E-4 0.0 10-11 0.0 0.0 0.0 1.1796640080972138E-4 0.0 12-13 0.0 0.0 0.0 1.1796640080972138E-4 0.0 14-15 0.0 0.0 0.0 1.1796640080972138E-4 0.0 16-17 0.0 0.0 0.0 2.3593280161944276E-4 0.0 18-19 0.0 0.0 0.0 2.3593280161944276E-4 0.0 20-21 0.0 0.0 0.0 3.538992024291641E-4 0.0 22-23 0.0 0.0 0.0 4.718656032388855E-4 0.0 24-25 0.0 0.0 0.0 7.077984048583282E-4 0.0 26-27 0.0 0.0 0.0 9.43731206477771E-4 0.0 28-29 0.0 0.0 0.0 0.0021233952145749847 0.0 30-31 0.0 0.0 0.0 0.005013572034413159 0.0 32-33 0.0 0.0 0.0 0.009201379263158269 0.0 34-35 0.0 0.0 0.0 0.011914606481781859 0.0 36-37 0.0 0.0 0.0 0.016869195315790158 0.0 38-39 0.0 0.0 0.0 0.025067860172065793 0.0 40-41 0.0 0.0 0.0 0.03491805463967753 0.0 42-43 0.0 0.0 0.0 0.048720123534414926 0.0 44-45 0.0 0.0 0.0 0.06110659561943567 0.0 46-47 0.0 0.0 0.0 0.08711818699797924 0.0 48-49 0.0 0.0 0.0 0.12415963685223175 0.0 50-51 0.0 0.0 0.0 0.14521663939676702 0.0 52-53 0.0 0.0 0.0 0.18272995485425841 0.0 54-55 0.0 0.0 0.0 0.24820130730365375 0.0 56-57 0.0 0.0 0.0 0.34487477276722045 0.0 58-59 0.0 0.0 0.0 0.4459129950607468 0.0 60-61 0.0 0.0 0.0 0.588121491236866 0.0 62-63 0.0 0.0 0.0 0.7292682898056975 0.0 64-65 0.0 0.0 0.0 0.8635140539271604 0.0 66-67 0.0 0.0 0.0 1.0618155736883022 0.0 68-69 0.0 0.0 0.0 1.326178277902888 0.0 70-71 0.0 0.0 0.0 1.4824247757753637 0.0 72-73 0.0 0.0 0.0 1.698244306056749 1.1796640080972138E-4 74-75 0.0 0.0 0.0 1.9487459581761923 1.1796640080972138E-4 76-77 0.0 0.0 0.0 2.2036123671255954 1.1796640080972138E-4 78-79 0.0 0.0 0.0 2.480125610623582 1.1796640080972138E-4 80-81 0.0 0.0 0.0 2.909582292771373 1.1796640080972138E-4 82-83 0.0 0.0 0.0 3.3975503097207858 1.1796640080972138E-4 84-85 0.0 0.0 0.0 3.956062234354411 1.1796640080972138E-4 86-87 0.0 0.0 0.0 4.660321647188448 1.1796640080972138E-4 88-89 0.0 0.0 0.0 5.465619282316011 1.1796640080972138E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3015 0.0 45.41483 1 GTATCAA 5100 0.0 37.382362 1 TATCAAC 6530 0.0 29.24979 2 ATCAACG 6590 0.0 28.91138 3 TCAACGC 6705 0.0 28.486372 4 CAACGCA 6725 0.0 28.334356 5 AACGCAG 6890 0.0 27.650902 6 GTACATG 5675 0.0 26.6412 1 TACCTGG 615 0.0 26.267193 2 TACATGG 5710 0.0 26.211044 2 ATGGGAG 1565 0.0 25.50505 5 ACATGGG 5675 0.0 25.368023 3 CCTATAC 225 1.0186341E-10 25.340115 3 ACGCAGA 7770 0.0 24.39842 7 CGCAGAG 7805 0.0 24.228138 8 ATAACGC 100 0.001650711 23.75636 3 TATAACG 100 0.001650711 23.75636 2 GAGTACT 4000 0.0 22.97884 12-13 CATGGGA 3935 0.0 22.699848 4 GCAGAGT 8135 0.0 22.602856 9 >>END_MODULE